CN106367323A - Continuous fermentation device for slime producing bacteria - Google Patents
Continuous fermentation device for slime producing bacteria Download PDFInfo
- Publication number
- CN106367323A CN106367323A CN201610821238.XA CN201610821238A CN106367323A CN 106367323 A CN106367323 A CN 106367323A CN 201610821238 A CN201610821238 A CN 201610821238A CN 106367323 A CN106367323 A CN 106367323A
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- CN
- China
- Prior art keywords
- bacteria
- aid
- continuous
- transfusion bottle
- fermentation device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a continuous fermentation device for slime producing bacteria. The continuous fermentation device comprises a hanging bottle, an upper cap, a lower cap and a collection bottle, openings are formed in the upper portion and the lower portion of the hanging bottle, the lower cap is provided with a conical top, the bacteria and a liquid culture medium are separated by the aid of bottleneck barrier action and properties of gel in a solid culture medium, continuous stable nutrient substances are provided for the bacteria by the aid of gravity action, bacterial agents are automatically collected by the aid of slime producing characteristics of the bacteria, the conical bare solid culture medium is manufactured by the aid of the lower cap, so that bacterial strains are conveniently inoculated, the bacterial agents are conveniently collected, glass and resin materials are selected, high-pressure steam sterilization can be achieved, combination of shapes is designed, and sterilization and sterile operations are facilitated. The continuous fermentation device can be used for fermentation production of slime producing bacteria, continuous fermentation production of the bacterial agents is achieved, the culture media keeps fresh, and fermentation processes of the slime producing bacteria are saved, continuous and facilitated.
Description
Technical field
The present invention relates to microbial apparatus field, especially a kind of continuing fermentation device producing slime bacteria.
Background technology
The media such as agaropectin can allow medium nutrient content to pass through, and stops bacterial cell from passing through.Existing bacterial fermentation
Technology, by the way of antibacterial and medium liquid mix, affects because antibacterial itself secretes the factors such as mortifier, through one section
Exponential phase is put into decline phase, and itself inhibiting substances of nutrient substance and bacterial secretory mix, and cause to avoid
Remaining nutrient substance waste, bacteria growth is also suppressed.Some antibacterials produce mucus, for example can with legume altogether
The root nodule bacteria of growing nitrogen-fixing, root nodule bacteria and legume synbiosiss can increase soil fertility, reduce fertilizer application, improve grain product
Matter, environmental protection, thus increasingly paid attention to.Some bacterium colonies such as actinomycetes are combined closely with solid medium, and some antibacterials
Bacterium colony is not then combined with solid medium.Prior art is difficult to separate growth of microorganism with nutrition, causes nutrient substance
Waste and sweat continue and the expenditure of labor more.
Content of the invention
The purpose of the present invention is: in order to overcome the above-mentioned deficiency of prior art, the present invention provides a kind of product slime bacteria
Continuing fermentation device, for producing the continuing fermentation of slime bacteria.
The technical solution adopted for the present invention to solve the technical problems is: a kind of continuing fermentation device producing slime bacteria,
Including transfusion bottle (1), upper lid (2), lower cover (3) and receiving flask (4), transfusion bottle (1) is made up of glass, has opening up and down, and middle part is justified
Cylindricality, upper lid (2) is suitable for reading with transfusion bottle (1) to be engaged, and it is suitable for reading to screw the salable transfusion bottle of lid (2) (1), and lower cover (3) top is circular cone
Shape, hollow, resin is made, and is engaged with mouth under transfusion bottle (1), screws mouth under the salable transfusion bottle of lower cover (3) (1), screws lower cover (3),
Pour solid medium into, after cooled and solidified, unscrew lower cover (3), exposed tapered solid culture medium can be formed, be easy to inoculation thin
Bacterium, receiving flask (4) is uncovered beaker shape, and glass is made, and internal diameter is less than cylindrical outer diameter in the middle part of transfusion bottle (1), can shelve transfusion bottle (1)
And collect the bacterial slime microbial inoculum dripping.
The invention has the beneficial effects as follows: block solid medium using transfusion bottle (1) bottleneck, using lower cover (3) conical top
Make the exposed solid medium of taper, be easy to inoculated bacteria, split antibacterial and culture medium nutrition using solid medium gel (5)
Material, is continued using action of gravity and lentamente provides nutrient substance for antibacterial, produces mucus property using antibacterial and collects antibacterial bacterium
Agent, the present invention can make full use of culture medium nutrient substance, reduces the waste of nutrient substance, convenient collection antibacterial microbial inoculum, reduces labor
Fatigue resistance, realizes microbial inoculum continuous production and culture medium keeps fresh pollution-free, realizes the saving of microbial inoculum production process, continues and just
Profit.
Brief description
The present invention is further described with reference to the accompanying drawings and examples.
Fig. 1 is the structural representation of one embodiment of the invention.
In figure 1. transfusion bottle, 2. on cover, 3. lower cover, 4. receiving flask, 5. gel.
Specific embodiment
In Fig. 1, transfusion bottle (1) is to have open circles cylindricality vial shape up and down, and upper lid (2) is resinite, suitable for reading with transfusion bottle (1)
Occlusion, lower cover (3) is resinite, is engaged with mouth under transfusion bottle (1), and lower cover (3) top is cone, and receiving flask (4) is cylinder
Glass beaker shape, internal diameter is less than transfusion bottle (1) external diameter, is easy to shelve transfusion bottle (1).
The work process of the diagram embodiment of the present invention is: a. is by transfusion bottle (1), upper lid (2), lower cover (3), receiving flask (4)
With high pressure steam sterilization, before sterilizing, lower cover (3) is tightened, upper lid (2) is unscrewed；B. prepare solid medium from transfusion bottle with agar
(1) place suitable for reading pours transfusion bottle (1) into flooding bottleneck below transfusion bottle (1)；C. unscrew lower cover after solid medium cooled and solidified
(3), gel (5) seals mouth and cervical region under transfusion bottle (1), and forms exposed tapered solid culture medium；D. connect producing slime bacteria
Plant in exposed tapered solid media surface；E. transfusion bottle (1) is shelved on receiving flask (4), adds from (1) toward transfusion bottle suitable for reading
Enter fluid medium and screw lid (2)；F. bacterial cell with mucus constantly lower, continuous production, in transfusion bottle (1), protect by culture medium
Hold fresh pollution-free.
Claims (1)
1. a kind of continuing fermentation device producing slime bacteria, including transfusion bottle (1), upper lid (2), lower cover (3) and receiving flask (4), hangs
Bottle (1) is made up of glass, has opening up and down, and middle part is cylindrical, and upper lid (2) is suitable for reading with transfusion bottle (1) to be engaged, lower cover (3) top
For cone, hollow, resin is made, and is engaged with mouth under transfusion bottle (1), and receiving flask (4) is uncovered beaker shape, and glass is made, internal diameter
Less than cylindrical outer diameter in the middle part of transfusion bottle (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610821238.XA CN106367323A (en) | 2016-09-13 | 2016-09-13 | Continuous fermentation device for slime producing bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610821238.XA CN106367323A (en) | 2016-09-13 | 2016-09-13 | Continuous fermentation device for slime producing bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106367323A true CN106367323A (en) | 2017-02-01 |
Family
ID=57897526
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610821238.XA Pending CN106367323A (en) | 2016-09-13 | 2016-09-13 | Continuous fermentation device for slime producing bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106367323A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109913370A (en) * | 2019-03-01 | 2019-06-21 | 杨贵珍 | A kind of microorganism reclaimer |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101148641A (en) * | 2007-08-06 | 2008-03-26 | 中国科学院南海海洋研究所 | High-flux fast screening method for antibiotics generated bacterium and device thereof |
| US20110300609A1 (en) * | 2008-06-27 | 2011-12-08 | Postech Academy-Industry Foundation | Nucleic acid extraction apparatus |
| CN102703308A (en) * | 2012-06-13 | 2012-10-03 | 福建省农业科学院植物保护研究所 | Bacteria filtering cup and application thereof to bacteriostatic activity detection of biocontrol bacteria metabolin |
| CN105875202A (en) * | 2016-06-13 | 2016-08-24 | 蔡志闯 | Inoculation mechanism, strain containers and method for culturing edible mushrooms |
-
2016
- 2016-09-13 CN CN201610821238.XA patent/CN106367323A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101148641A (en) * | 2007-08-06 | 2008-03-26 | 中国科学院南海海洋研究所 | High-flux fast screening method for antibiotics generated bacterium and device thereof |
| US20110300609A1 (en) * | 2008-06-27 | 2011-12-08 | Postech Academy-Industry Foundation | Nucleic acid extraction apparatus |
| CN102703308A (en) * | 2012-06-13 | 2012-10-03 | 福建省农业科学院植物保护研究所 | Bacteria filtering cup and application thereof to bacteriostatic activity detection of biocontrol bacteria metabolin |
| CN105875202A (en) * | 2016-06-13 | 2016-08-24 | 蔡志闯 | Inoculation mechanism, strain containers and method for culturing edible mushrooms |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109913370A (en) * | 2019-03-01 | 2019-06-21 | 杨贵珍 | A kind of microorganism reclaimer |
| CN109913370B (en) * | 2019-03-01 | 2022-08-09 | 郭聃洋 | Microorganism recovery plant |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| AD01 | Patent right deemed abandoned | ||
| AD01 | Patent right deemed abandoned |
Effective date of abandoning: 20211001 |