Content of the invention
It is an object of the present invention to provide a kind of implantation methods reducing Oryza sativa L. heavy metal chromium content, at animals and plants enrichment
Reason, leaching process, adsorption treatment, microbial treatments, change water to process and rice field management, both can reduce the huge sum of money at each position of Oryza glutinosa
Belong to chromium content, reduce soil in heavy metal chromium content, again can remediating heavy metal chromium-polluted soil, be suitable for soil be subject to a huge sum of money
Belong to the regional rice cultivation application of pollution of chromium.
In order to realize according to object of the present invention and further advantage, there is provided a kind of reduction Oryza sativa L. heavy metal chromium content
Implantation methods, comprise the following steps:
S1, animals and plants enrichment are processed: conventionally carry out accelerating germination of rice seed nursery, then plant rice seedling
In rice field, 3~5 days after rice seedling plantation, plant Herba Oenanthes Javanicae seedling in rice field near the edge of ridge, plantation print on ridge
Degree Caulis et Folium Brassicae junceae seedling, sprayed an auxin every 7~10 days to Herba Oenanthes Javanicae seedling and Indian mustard seedling, at paddy field water bottom
Cultivation hydriopsis cumingii;Before described Herba Oenanthes Javanicae seedling, Indian mustard seedling cultivation, concentration is 0.05mg/ml vitamin b12Solution
Its root is soaked 10~15min;
S2, leaching are processed: 30~35 days before plantation rice shoot, according to mass ratio 2.2~3.2:2.5~2.8:1.8~2.5:1
Fatty acid methyl ester APEO, fatty acid methyl ester ethoxylate sulfonate, dodecylbenzene sodium sulfonate and rhamnose
Fat compounds and makes leaching agent, soil in the 20~30cm thickness of rice field is taken out and carries out leaching process using leaching agent and water to it,
Then the paddy soil after the completion of leaching is reverted to recuperation in rice field;Described leaching process in leaching agent, water, paddy soil
Weight is than for 1:800~1000:800~1200;
S3, adsorption treatment: 5~7 days after rice seedling plantation, the crosslinking that mass ratio is 3~4:2~3:2.5~3.5:4
Cationic starch chelating agen granule, strongly acidic styrene type cation exchange resin granule, chitosan derivative composition granule, activated carbon
The mixture of granule loads nylon wire and makes chromium absorbent packet, the bottom in the rice seedling space in embedment rice field, every mu of rice field embedment
200~250 chromium absorbent packets, took out chromium absorbent packet and carried out desorbing and cleaning every 7~10 days, then again imbedded rice field;
S4, microbial treatments: from the beginning of rice ear sprouting period, uniformly throw in 0.2 in 8~10 days to every mu paddy field water~
The microalgae living body of 0.4kg, the cattle colostrumss of 10~20kg and 50~100g microbial bacteria, stop to rice harves;
S5, change water to process: be completed to rice ear sprouting period from rice seedling plantation, every 5~7 days, the water in rice field carried out
Once change;During from rice ear sprouting period to rice harves, before throwing in microalgae living body, cattle colostrumss and microbial bacteria every time, right
Water in rice field is carried out once with changing;
S6, rice field management: 6~8 days after rice seedling plantation, spray 1500~1800 times of 20% Acetochlor wettable powder
Liquid carries out weeding, and after 7~9 days, repeat spraying is once;40~60 Rana nigromaculatas, 30~50 Bufo siccuss are put in every mu of rice field;
Rice blast occurs during seedling growth, immediately can with 40% 300~500 times of liquid of sulfur ring azole suspending agent or 75% tricyclazole
1800~2200 times of liquid of WP are sprayed.
Preferably, in s1 leaching process method be: using volume be 200l, mixing speed be 200~300r/min
Stirring reactor leaching process is carried out to paddy soil.
Preferably, in s3, the aperture of nylon wire is less than Cross-linking Cationic Starch chelating agen granule, strongly acidic styrene system
The particle diameter of smallest particles in cation exchange resin granule, chitosan derivative composition granule, activated carbon granule.
Preferably, in s3, chromium absorbent packet carries out desorbing and the method for cleaning is: by the cross-linked cationic in chromium absorbent packet
Starch chelating agen granule, strongly acidic styrene type cation exchange resin granule, chitosan derivative composition granule, activated carbon granule take
Go out, soak 2~4h with the hydrochloric acid solution of 1mol/l, then use the acetum of 0.5mol/l to soak 4~6h, more clear with distilled water
Wash 3~5 times, use new nylon wire instead and again pack.
Preferably, in s4 microalgae living body include Chlorococcum that mass ratio is 0.8~1:1.2~1.5:0.5:1.3:1.8,
Shank algae, chlorella, boat-shaped algae and spirulina plalensiss.
Preferably, in s4, microbial bacteria includes mass ratio is the Sphaerotilus natans of 1~2:1.5~2.5:0.8:1.2, product
Protein candidiasis, bacillus subtilises and Candida lipolytica bacterium.
The present invention at least includes following beneficial effect:
Firstth, concentration is 0.05mg/ml vitamin b12The root of Herba Oenanthes Javanicae seedling, Indian mustard seedling is soaked by solution
Bubble, then plants Herba Oenanthes Javanicae seedling in rice field near the edge of ridge, plants Indian mustard seedling, every 7~10 days on ridge
Spray an auxin;Herba Oenanthes Javanicae seedling, Indian mustard seedling pass through vitamin b12After solution leaching root, root growth is developed
Vigorous so as to start, with regard to heavy metal chromium, there is very strong enrichment from Seedling Stage, then it is strengthened by auxin
Growth, accelerates to heavy metal-polluted soil chrome uptake, thus effectively reducing the heavy metal chromium content of paddy soil;Support at paddy field water bottom
Grow hydriopsis cumingii, also can effectively absorb heavy metal chromium in paddy field water, reduce rice field heavy metal chromium content further;
Secondth, fatty acid methyl ester APEO, fatty acid methyl ester ethoxylate sulfonate, dodecylbenzene sodium sulfonate
And this several surfactant compound of rhamnolipid make leaching agent, wherein fatty acid methyl ester APEO, fatty acid methyl
Ester ethoxylate sulfonates and rhamnolipid are all the surfactants of environmental type, by the complexation of surfactant
Effect, the heavy metal in paddy soil is separated, and reaches effective purpose reducing heavy metal-polluted soil chromium content;
3rd, Cross-linking Cationic Starch chelating agen granule, strongly acidic styrene type cation exchange resin granule, shitosan
Derivative particles, activated carbon granule all have the ability of good Adsorption of Heavy Metals chromium;Strongly acidic styrene's cation exchanges
Resin particle can carry out with heavy metal chromium ion chelating, exchange reaction, adsorb heavy metal chromium in soil, have reversible simultaneously
Property, can be reused by regeneration;Activated carbon also can active adsorption heavy metal chromium, also can reuse well after cleaning;
4th, Chlorococcum, shank algae, chlorella, boat-shaped algae and these algae of spirulina plalensiss have stronger absorption
The ability of heavy metal chromium, due to there being one layer of emplastic outside the cell wall of these algae, this kind of material is because having containing alduronic acid
There is the ability of very big combination heavy metal chromium ion, algae adsorbent also has low cost, good selective, very simultaneously
It is properly applied to the high rice field of content of beary metal;
5th, Sphaerotilus natans, Candida utilis, bacillus subtilises and Candida lipolytica bacterium are to weight in water
The impact of metal is mainly reflected in biological adsorption and enrichment;Sphaerotilus natans heavy metal chromium has very strong degraded and absorption
Ability, can also gather beta-hydroxy-butanoic acid simultaneously, can improve water quality of paddy field;Candida utilis, bacillus subtilises and
Candida lipolytica bacterium all has the ability of preferable Adsorption of Heavy Metals chromium, metal chromium ions can with its cell surface on the moon from
The cell wall heavy metal chromium that son interacts and fixed has preferable absorbability.
Part is embodied by the further advantage of the present invention, target and feature by description below, and part also will be by this
Invention research and practice and be understood by the person skilled in the art.
Specific embodiment
With reference to embodiment, the present invention is elaborated, to make those of ordinary skill in the art refer to after this specification
Can implement according to this.
<embodiment 1>
A kind of implantation methods reducing Oryza sativa L. heavy metal chromium content, comprise the following steps:
S1, animals and plants enrichment are processed: conventionally carry out accelerating germination of rice seed nursery, then plant rice seedling
In rice field, rice seedling is planted latter 3 days, plants Herba Oenanthes Javanicae seedling in rice field near the edge of ridge, plants India on ridge
Caulis et Folium Brassicae junceae seedling, sprayed an auxin every 7 days to Herba Oenanthes Javanicae seedling and Indian mustard seedling, cultivated three at paddy field water bottom
Angle sail freshwater mussel;Before described Herba Oenanthes Javanicae seedling, Indian mustard seedling cultivation, concentration is 0.05mg/ml vitamin b12Solution is by its root
10min is soaked in portion;The method that described leaching is processed is: using volume be 200l, mixing speed be 200r/min stirring-type anti-
Device is answered to carry out leaching process to paddy soil;
S2, leaching are processed: first 30 days of plantation rice shoot, according to the fatty acid methyl ester polyoxy second of mass ratio 2.2:2.5:1.8:1
Alkene ether, fatty acid methyl ester ethoxylate sulfonate, dodecylbenzene sodium sulfonate and rhamnolipid compound and make leaching agent, will
In the 20cm thickness of rice field, soil takes out and carries out leaching process using leaching agent and water to it, then by the paddy soils after the completion of leaching
Earth reverts to recuperation in rice field;Described leaching process in leaching agent, water, the weight ratio of paddy soil for 1:800:800;
S3, adsorption treatment: rice seedling is planted latter 5 days, by the Cross-linking Cationic Starch chelating for 3:2:2.5:4 for the mass ratio
Agent granule, strongly acidic styrene type cation exchange resin granule, chitosan derivative composition granule, the mixture dress of activated carbon granule
Enter nylon wire and make chromium absorbent packet, the bottom in the rice seedling space in embedment rice field, 200 chromium absorbent packets are imbedded in every mu of rice field,
Every 7 days take out chromium absorbent packet, by the Cross-linking Cationic Starch chelating agen granule in chromium absorbent packet, strongly acidic styrene system sun from
Sub-exchange resin granule, chitosan derivative composition granule, activated carbon granule take out, and soak 2h with the hydrochloric acid solution of 1mol/l, then
Soak 4h with the acetum of 0.5mol/l, then cleaned with distilled water 3 times, use new nylon wire instead and again pack, then again bury
Enter rice field;The aperture of described nylon wire is less than Cross-linking Cationic Starch chelating agen granule, strongly acidic styrene's cation exchanges
The particle diameter of smallest particles in resin particle, chitosan derivative composition granule, activated carbon granule;
S4, microbial treatments: from the beginning of rice ear sprouting period, uniformly throw in the micro- of 0.2kg in 8 days to every mu paddy field water
Algae live body, the cattle colostrumss of 10kg and 50g microbial bacteria, stop to rice harves;Described microalgae living body includes mass ratio
The Chlorococcum of 0.8:1.2:0.5:1.3:1.8, shank algae, chlorella, boat-shaped algae and spirulina plalensiss;Described microbial bacteria includes
Mass ratio is Sphaerotilus natans, Candida utilis, bacillus subtilises and the Candida lipolytica of 1:1.5:0.8:1.2
Bacterium;
S5, change water to process: be completed to rice ear sprouting period from rice seedling plantation, every 5 days, one carried out to the water in rice field
Secondary replacing;During from rice ear sprouting period to rice harves, before throwing in microalgae living body, cattle colostrumss and microbial bacteria every time, to rice
The water of Tanaka is carried out once with changing;
S6, rice field management: rice seedling is planted latter 6 days, sprays 1500 times of liquid of 20% Acetochlor wettable powder and is removed
Grass, after 7 days, repeat spraying is once;40 Rana nigromaculatas, 30 Bufo siccuss are put in every mu of rice field;Occur during seedling growth
Rice blast, is sprayed with 40% 300 times of liquid of sulfur ring azole suspending agent or 1800 times of liquid of 75% tricyclazole wettable powder immediately
Mist.
<embodiment 2>
A kind of implantation methods reducing Oryza sativa L. heavy metal chromium content, comprise the following steps:
S1, animals and plants enrichment are processed: conventionally carry out accelerating germination of rice seed nursery, then plant rice seedling
In rice field, rice seedling is planted latter 5 days, plants Herba Oenanthes Javanicae seedling in rice field near the edge of ridge, plants India on ridge
Caulis et Folium Brassicae junceae seedling, sprayed an auxin every 10 days to Herba Oenanthes Javanicae seedling and Indian mustard seedling, in the cultivation of paddy field water bottom
Hydriopsis cumingii;Before described Herba Oenanthes Javanicae seedling, Indian mustard seedling cultivation, concentration is 0.05mg/ml vitamin b12Solution by its
Root soaks 15min;The method that described leaching is processed is: using volume be 200l, mixing speed be the stirring-type of 300r/min
Reactor carries out leaching process to paddy soil;
S2, leaching are processed: first 35 days of plantation rice shoot, according to the fatty acid methyl ester polyoxy second of mass ratio 3.2:2.8:2.5:1
Alkene ether, fatty acid methyl ester ethoxylate sulfonate, dodecylbenzene sodium sulfonate and rhamnolipid compound and make leaching agent, will
In the 30cm thickness of rice field, soil takes out and carries out leaching process using leaching agent and water to it, then by the paddy soils after the completion of leaching
Earth reverts to recuperation in rice field;Described leaching process in leaching agent, water, the weight ratio of paddy soil for 1:1000:1200;
S3, adsorption treatment: rice seedling is planted latter 7 days, by the Cross-linking Cationic Starch chelating for 4:3:3.5:4 for the mass ratio
Agent granule, strongly acidic styrene type cation exchange resin granule, chitosan derivative composition granule, the mixture dress of activated carbon granule
Enter nylon wire and make chromium absorbent packet, the bottom in the rice seedling space in embedment rice field, 250 chromium absorbent packets are imbedded in every mu of rice field,
Took out chromium absorbent packet every 10 days, the Cross-linking Cationic Starch chelating agen granule in chromium absorbent packet, strongly acidic styrene are sun
Ion-exchange resin particles, chitosan derivative composition granule, activated carbon granule take out, and soak 4h with the hydrochloric acid solution of 1mol/l, so
The acetum using 0.5mol/l afterwards soaks 6h, then is cleaned with distilled water 5 times, uses new nylon wire instead and again packs, then again
Embedment rice field;The aperture of described nylon wire is less than Cross-linking Cationic Starch chelating agen granule, strongly acidic styrene's cation is handed over
Change resin particle, chitosan derivative composition granule, in activated carbon granule smallest particles particle diameter;
S4, microbial treatments: from the beginning of rice ear sprouting period, uniformly throw in 0.4kg's in 10 days to every mu paddy field water
Microalgae living body, the cattle colostrumss of 20kg and 100g microbial bacteria, stop to rice harves;Described microalgae living body includes mass ratio
Chlorococcum for 1:1.5:0.5:1.3:1.8, shank algae, chlorella, boat-shaped algae and spirulina plalensiss;Described microbial bacteria includes
Mass ratio is Sphaerotilus natans, Candida utilis, bacillus subtilises and the Candida lipolytica of 2:2.5:0.8:1.2
Bacterium;
S5, change water to process: be completed to rice ear sprouting period from rice seedling plantation, every 7 days, one carried out to the water in rice field
Secondary replacing;During from rice ear sprouting period to rice harves, before throwing in microalgae living body, cattle colostrumss and microbial bacteria every time, to rice
The water of Tanaka is carried out once with changing;
S6, rice field management: rice seedling is planted latter 8 days, sprays 1800 times of liquid of 20% Acetochlor wettable powder and is removed
Grass, after 9 days, repeat spraying is once;60 Rana nigromaculatas, 50 Bufo siccuss are put in every mu of rice field;Occur during seedling growth
Rice blast, is sprayed with 40% 500 times of liquid of sulfur ring azole suspending agent or 2200 times of liquid of 75% tricyclazole wettable powder immediately
Mist.
<embodiment 3>
A kind of implantation methods reducing Oryza sativa L. heavy metal chromium content, comprise the following steps:
S1, animals and plants enrichment are processed: conventionally carry out accelerating germination of rice seed nursery, then plant rice seedling
In rice field, rice seedling is planted latter 4 days, plants Herba Oenanthes Javanicae seedling in rice field near the edge of ridge, plants India on ridge
Caulis et Folium Brassicae junceae seedling, sprayed an auxin every 8 days to Herba Oenanthes Javanicae seedling and Indian mustard seedling, cultivated three at paddy field water bottom
Angle sail freshwater mussel;Before described Herba Oenanthes Javanicae seedling, Indian mustard seedling cultivation, concentration is 0.05mg/ml vitamin b12Solution is by its root
12min is soaked in portion;The method that described leaching is processed is: using volume be 200l, mixing speed be 250r/min stirring-type anti-
Device is answered to carry out leaching process to paddy soil;
S2, leaching are processed: first 32 days of plantation rice shoot, according to the fatty acid methyl ester polyoxy second of mass ratio 2.7:2.6:2.1:1
Alkene ether, fatty acid methyl ester ethoxylate sulfonate, dodecylbenzene sodium sulfonate and rhamnolipid compound and make leaching agent, will
In the 25cm thickness of rice field, soil takes out and carries out leaching process using leaching agent and water to it, then by the paddy soils after the completion of leaching
Earth reverts to recuperation in rice field;Described leaching process in leaching agent, water, the weight ratio of paddy soil for 1:900:1000;
S3, adsorption treatment: rice seedling is planted latter 6 days, by the Cross-linking Cationic Starch chela for 3.5:2.5:3:4 for the mass ratio
Mixture granule, strongly acidic styrene type cation exchange resin granule, chitosan derivative composition granule, the mixture of activated carbon granule
Load nylon wire and make chromium absorbent packet, the bottom in the rice seedling space in embedment rice field, 225 chromium absorption are imbedded in every mu of rice field
Bag, took out chromium absorbent packet every 8 days, by the Cross-linking Cationic Starch chelating agen granule in chromium absorbent packet, strongly acidic styrene system
Cation exchange resin granule, chitosan derivative composition granule, activated carbon granule take out, and soak 3h with the hydrochloric acid solution of 1mol/l,
Then the acetum using 0.5mol/l soaks 5h, then is cleaned with distilled water 4 times, uses new nylon wire instead and again packs, Ran Houchong
New embedment rice field;The aperture of described nylon wire is less than Cross-linking Cationic Starch chelating agen granule, strongly acidic styrene's cation
The particle diameter of smallest particles in exchanger resin granule, chitosan derivative composition granule, activated carbon granule;
S4, microbial treatments: from the beginning of rice ear sprouting period, uniformly throw in the micro- of 0.3kg in 9 days to every mu paddy field water
Algae live body, the cattle colostrumss of 15kg and 80g microbial bacteria, stop to rice harves;Described microalgae living body includes mass ratio
The Chlorococcum of 0.9:1.3:0.5:1.3:1.8, shank algae, chlorella, boat-shaped algae and spirulina plalensiss;Described microbial bacteria includes
Mass ratio is Sphaerotilus natans, Candida utilis, bacillus subtilises and the Candida lipolytica of 1.5:2:0.8:1.2
Bacterium;
S5, change water to process: be completed to rice ear sprouting period from rice seedling plantation, every 6 days, one carried out to the water in rice field
Secondary replacing;During from rice ear sprouting period to rice harves, before throwing in microalgae living body, cattle colostrumss and microbial bacteria every time, to rice
The water of Tanaka is carried out once with changing;
S6, rice field management: rice seedling is planted latter 7 days, sprays 1600 times of liquid of 20% Acetochlor wettable powder and is removed
Grass, after 8 days, repeat spraying is once;50 Rana nigromaculatas, 40 Bufo siccuss are put in every mu of rice field;Occur during seedling growth
Rice blast, is sprayed with 40% 400 times of liquid of sulfur ring azole suspending agent or 2000 times of liquid of 75% tricyclazole wettable powder immediately
Mist.
<contrast test>
In the higher region of heavy metal chromium content, choose 4 block size identical rice fields, wherein 3 pieces rice fields are according to embodiment 1
~3 method rice cultivation, remaining 1 piece of rice field uses traditional method rice cultivation as a comparison case 1, detects before rice cultivation
Paddy soil heavy metal chromium content, detects paddy soil heavy metal chromium content again after rice harves, detect each portion of Oryza sativa L. simultaneously
The heavy metal chromium content of position, result of the test is as follows:
Table 1 Oryza sativa L. and heavy metal chromium in soil content (mg/kg)
|
Soil before plantation |
Soil after plantation |
Root |
Stem and leaf |
Rice husk |
Rice |
Comparative example 1 |
367.48 |
320.61 |
10.12 |
6.24 |
3.63 |
3.44 |
Embodiment 1 |
367.49 |
140.12 |
3.32 |
1.56 |
0.46 |
0.32 |
Embodiment 2 |
367.52 |
139.83 |
3.23 |
1.37 |
0.43 |
0.28 |
Embodiment 3 |
367.53 |
139.42 |
3.12 |
1.24 |
0.41 |
0.24 |
As can be seen from Table 1, soil Cr content before plantation, is 367.49~367.53mg/kg in embodiment 1~3, comparative example
1 is 367.48mg/kg;Soil Cr content after plantation, is 139.42~140.12mg/kg in embodiment 1~3, and comparative example 1 is
320.61mg/kg;It can be seen that according to the method for embodiment 1~3, the heavy metal chromium that can significantly reduce paddy soil contains
Amount;In embodiment 1~3, root chromium content is 3.12~3.32mg/kg, less than the 10.12mg/kg of comparative example 1;Embodiment 1~3
Middle stem and leaf part chromium content is 1.24~1.56mg/kg, less than the 6.24mg/kg of comparative example 1;In embodiment 1~3, rice husk chromium contains
Measure as 0.41~0.46mg/kg, less than the 3.63mg/kg of comparative example 1;In embodiment 1~3 rice chromium content be 0.24~
0.32mg/kg, meets national standard, less than the 3.44mg/kg of comparative example 1;Have by the Oryza sativa L. of present invention plantation and both can reduce
The heavy metal chromium content at each position of Oryza glutinosa can reduce heavy metal chromium content and energy remediating heavy metal pollution of chromium soil in soil again
Earth, the regional rice cultivation being suitable for being subject to heavy metal chromium pollution in soil is applied.
Although embodiment of the present invention is disclosed as above, it is not restricted to listed in description and embodiment
With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily
Realize other modification, therefore under the general concept being limited without departing substantially from claim and equivalency range, the present invention does not limit
In specific details with shown here as the embodiment with description.