CN106350449B - A kind of detection and screening system for cord blood stem cell - Google Patents
A kind of detection and screening system for cord blood stem cell Download PDFInfo
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- CN106350449B CN106350449B CN201610576302.2A CN201610576302A CN106350449B CN 106350449 B CN106350449 B CN 106350449B CN 201610576302 A CN201610576302 A CN 201610576302A CN 106350449 B CN106350449 B CN 106350449B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5073—Stem cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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Abstract
The invention discloses a kind of detection for cord blood stem cell and screening system, which includes：Collector isolates and purifies kit, cell cultivation instrument, refrigerated centrifuge, Hematology analyzer, information collection apparatus, printing equipment.The present invention by collector, isolate and purify kit, cell cultivation instrument, refrigerated centrifuge, Hematology analyzer acquired Cord blood made to obtain simply and accurately detection and screening while, also optimize detecting step and process, so that the Cord blood detection after acquisition is more rapidly, efficiently and in time, significantly reduce testing cost, not only the wasting of resources is avoided, but also reduces detection total time.
Description
Technical field
The present invention originally belongs to footprint detection technique field more particularly to a kind of detection and screening for cord blood stem cell
System.
Background technology
Stem cell (Stem Cells, SC) is that a kind of pluripotency with the of self-replication capacity (self-renewing) is thin
Born of the same parents are the cells of original and non-specialization.Under certain condition, it can be divided into multiple functions cell, have various groups of regeneration
Knit the potential function of organ and human body.Stem cell can be divided via mitosis with differentiation there are in all multicellular tissues
At a variety of specialized cells, and more stem cells can be provided using self-renewing.The source of stem cell has very much, including
Cord blood and marrow.For mammal, stem cell be divided into embryonic stem cell (Embryonic Stem Cell, ESC) at
Somatic stem cell (Adult Stem Cell, ASC) two major classes, embryonic stem cell are derived from the inner cell mass in blastaea；It is thin into soma
Born of the same parents then come from miscellaneous tissue.
The blood being retained in after baby due in placenta and umbilical cord is the important sources of stem cell.Hematopoietic Stem in Cord blood is thin
Born of the same parents can be used for treating a variety of diseases in the blood system and disease of immune system.Cord blood can not only effectively treat tens kinds of difficulties
The property controlled disease and a variety of incurable diseases, and the kinds of Diseases that it can be treated also are being constantly increasing.The umbilical cord stored self
Blood is once need in use, being not required to distribution type, and cell activity is strong, the danger of no immunological rejection, and transplanting success is high, and cure rate is high,
Medical expense is low.
So one kind can after guarantee period umbilical cord detaches with puerpera and baby, can in time, quickly, it is simple and accurately obtain
The system of detection and screening is particularly necessary.
Invention content
The embodiment of the present invention is designed to provide a kind of detection for cord blood stem cell and screening system, it is intended to carry
For one kind can after guarantee period umbilical cord detaches with puerpera and baby, can in time, quickly, it is simple and accurately obtain detection and screening
System.
The embodiment of the present invention is achieved in that a kind of detection for cord blood stem cell and screening method include：
Step 1: contained Cord blood in umbilical cord is acquired, dispensed and sampled, and will acquisition information and working condition biography
It send to center control and processing equipment；
Step 2: the cord blood stem cell information to acquisition carries out radio frequency identification RFID pre-configuration；
Step 3: analyzing preconfigured radio frequency identification RFID, the unique mark UID of the RFID is determined, it will
The parameter information of the UID and the carrier of the RFID that determine are combined, and generate anti-counterfeiting information；
Step 4: carrying out stem cell population, competence for added value, micro organism quantity to the cord blood stem cell for generating anti-counterfeiting information
Detection；
Step 5: carrying out epidemiology detection and the detection of HLA tissue matchings to the cord blood stem cell for generating anti-counterfeiting information；
Step 6: the part cell after step 5 and step 5 detection is carried out primary separation, culture amplification, induction
Break up or dedifferente processing, and the cellular informatics that record that treated；
Step 7: to carrying out cell Sterility testing through step 6 treated cell and biological nature detects, it will monitoring letter
Breath is signed according to preconfigured private key, generates anti-counterfeiting information signature；
Step 8: Anti-fake electronic label is written in anti-counterfeiting information signature and the parameter information, interrupt control unit will
Including the Anti-fake electronic label of cellular informatics is uploaded to remote server.
Further, the method for generating anti-counterfeiting information signature is that cipher key store central controller generates random number rkeyid, utilization is ellipse
Circular curve cryptographic algorithm calculates its corresponding public key rPKid, rPKid=rkeyid× G, G are the basic point of elliptic curve, by rPKidWith
User identifier UID is externally sent, and key generation system generates random number rkeyKMC, its phase is calculated using elliptic curve cryptography
The public key rPK answeredKMC, wherein rPKKMC=rkeyKMC× G, and remember γid=rPKid+rPKKMC, specially：
A, identity private key key is calculated using user identifier UIDidWith mark public key Rid, specially：
B, it is just that the size of generation private key matrix and public key matrix, private key matrix and public key matrix, which is m × h, m and h,
Integer；
Step 3: the hashed value of user identifier UID is calculated using hashing algorithm using user identifier UID, by the hash
Value is divided into m sections, as private key matrix and public key matrix column mapping value map [i], i=0, and 1,2......m-1；
It calculates：
ri,map[i]For an element in private key matrix；
Xi,map[i]For an element in public key matrix；
N is the rank of elliptic curve.
A kind of detection and screening system for cord blood stem cell, the system include：Collector isolates and purifies reagent
Box, cell cultivation instrument, refrigerated centrifuge, Hematology analyzer, information collection apparatus, printing equipment；
The collector is connect with kit is isolated and purified, and the cell cultivation instrument isolates and purifies reagent with described
Box connects, and the refrigerated centrifuge is arranged two, is connected respectively at cell cultivation instrument, the Hematology analyzer and institute
The refrigerated centrifuge connection stated, the information collection apparatus are connect with the Hematology analyzer, printing dress
It sets and is connect with the information collection apparatus.
Further, the collector uses the closed collection Cord Blood of Neonates of tetrad blood taking bag.
Further, described isolate and purify has separating liquid inside kit, separating liquid and bleeding of the umbilicus 4:1 proportional arrangement；
Further, the refrigerated centrifuge, which is divided into, prepares centrifuge and work centrifuge.
Further, the full cytoanalyze can detect the packet cell in bleeding of the umbilicus, red blood cell, hemoglobin, lymph
After cell percentages, monocyte percentage and extraction stem cell, mononuclearcell number；
Further, the result after full cytoanalyze and stem cell viability examination is uploaded to by the information collection apparatus
Server and it is sent to printing equipment；Described information collection device is provided with central control and processing equipment；The collector is adopted
Information is acquired with fibre optical sensor；The fibre optical sensor includes that light source generates part, gas chamber, signal control portion,
Light source generates part and is made of laser, single mode bidirectional optical fiber coupler, optical splitter, fiber grating, and signal control portion is by light
Electric explorer, lock-in amplifier, data collecting card, wavelengt demodulator are constituted, and laser sends out broadband light, and broadband light is double through single mode
Fiber grating is reached after to fiber coupler and obtains narrowband emergent light, and narrowband emergent light is divided into two-way through optical splitter, passes through all the way
Photodetector in signal control portion is reached after gas chamber, the output signal of photodetector reaches after lock-in amplifier amplifies
Data collecting card；Another way obtains centre wavelength value through wavelengt demodulator, and centre wavelength value is acquired by the data of signal control part
Card acquisition.
Data collecting card information collecting method is：
First, echo signal x (t) is acquired within the independent sampling period with awareness apparatus, A/D modes pair is used in combination
Signal carries out digital quantization；Then, dimensionality reduction is carried out to the signal x (i) after quantization；Finally, the signal after dimensionality reduction is reconstructed；
Wherein t is sampling instant, and i is the signal sequence after quantization；
Signal after described pair of quantization carries out dimensionality reduction, specifically passes through finite impulse response filter to the signal after quantization
Difference equation
Wherein h (0) ..., h (L-1) are filter coefficient, design the compressed sensing signal acquisition frame based on filtering, structure
Make following Teoplitz calculation matrix：
Then observeI=1 ..., M, wherein b1,…,bLRegard filter coefficient as；Submatrix ΦFT
Singular value be gram matrix G (ΦF, T) and=Φ 'FTΦFTThe arithmetic root of characteristic value, all eigenvalue λs of verification G (Φ F, T)
i∈(1-δK,1+δK), i=1 ..., T, then ΦFMeet RIP, and by solving following l1Optimization problem reconstructs original signal：
Original signal, that is, BP algorithm are reconstructed by linear programming method；
For actual compression signal Φ is then changed such as the acquisition of voice or picture signalFFor following form：
If signal has sparsity on transformation basic matrix Ψ, by solving following l1Optimization problem, accurate weight
Structure goes out original signal：
Wherein Φ is uncorrelated to Ψ, and Ξ is known as CS matrixes.
Further, described that contained Cord blood in umbilical cord is acquired, dispensed and sampled, and will acquisition information and work shape
State is sent to acquisition information in center control and processing equipment and is sent in center control and processing equipment by wireless routing, nothing
Circuit includes by the communication means communicated：
A, user equipment sends hypertext transfer protocol http request to wireless routing device, obtains certificate server and returns
Portal certification pages redirect response；N number of TD-SCDMA modules, N >=2 are configured in the wireless routing device；
User equipment obtains hot spot signal in the wireless signal coverage area of wireless routing device, carries out network connection；
The wireless routing device issues IP address to the user equipment；The IP address is the source address of the user equipment；Institute
It states user equipment and sends http request to the wireless routing device, the http request includes the source address；It is described wireless
The http request is forwarded to gateway server by routing device；The http request is forwarded to certification by the gateway server
Server；Portal certification pages are redirected response and return to the gateway server by the certificate server；The gateway clothes
Portal certification pages are redirected response and return to the wireless routing device by business device；The wireless routing device recognizes Portal
Card page jump response returns to the user equipment；
B, the user equipment to the wireless routing device send http logging requests, the http logging requests via
Gateway server is forwarded to the certificate server, is authenticated to the http logging requests by the certificate server；
After certification passes through, the certificate server carries out charging to the internet business of the user equipment；
The user equipment sends http logging requests to the wireless routing device；The http logging requests carry
Account and encrypted message；The http logging requests are forwarded to the gateway server by the wireless routing device；The net
It closes server and the http logging requests is forwarded to the certificate server；The certificate server is according to the account and close
Code information, is authenticated the http logging requests；After the authentication has been successful, the certificate server is to the gateway server
Return authentication successful respond, and start to carry out charging to the internet business of the user equipment；
The certification successful respond is returned to the wireless routing device by the gateway server, and records account certification
Information；The account authentication information includes the account and authentication state information of the user equipment；The wireless routing device will
The certification successful respond returns to the user equipment, and records the account authentication information；
C, the user equipment sends network access request to the wireless routing device；The wireless routing device according to
The resource load situation of N number of TD-SCDMA modules, a TD- for selecting resource load minimum by load balancing operation
SCDMA modules provide bandwidth resources for the network access request；The minimum TD-SCDMA modules of the resource load will be described
Network access request is forwarded to the destination address server to be accessed, and the network that the destination address server is returned is visited
Ask that response sends the user equipment to；
The user equipment initiates network access request to the wireless routing device；The network access request includes institute
State source address and the destination address to be accessed；The account authentication information of the wireless routing device inquiry local record, is verified
The account authentication state of the user equipment；If account authentication state is pass flag, the wireless routing device is in
The resource load situation for the N number of TD-SCDMA modules set, a TD- for selecting resource load minimum by load balancing operation
SCDMA modules provide bandwidth resources for the network access request；The minimum TD-SCDMA modules of the resource load will be described
Network access request is forwarded to the destination address server to be accessed；The server of the destination address is to the wireless routing
Equipment returns to network and accesses response；Network access response is returned to the user equipment by the wireless routing device；Institute
It states wireless routing device and sends heartbeat request to the gateway server；The heartbeat request is transmitted to by the gateway server
The certificate server；The certificate server returns to heartbeat response to the gateway server；The gateway server according to
The account authentication information of user equipment described in the content update of the heartbeat response；
D, the user equipment to the wireless routing device send http ask offline, the http offline request via
The gateway server is forwarded to the certificate server, and the certificate server stops the internet business to the user equipment
Carry out charging；
The user equipment sends http to the wireless routing device and asks offline；The wireless routing device will be described
Request is forwarded to the gateway server to http offline；By the http, request is forwarded to and described recognizes the gateway server offline
Demonstrate,prove server；The certificate server carries out processed offline to the user equipment；The certificate server takes to the gateway
Business device returns to offline successful respond, and stops carrying out charging to the internet business of the user equipment；The gateway server will
The offline successful respond returns to the wireless routing device, and deletes the account authentication information of local record；It is described wireless
The offline successful respond is returned to the user equipment by routing device, and deletes the account authentication information of local record.
Further, the printing equipment can print bar code and analysis report.
The present invention by collector, isolate and purify kit, cell cultivation instrument, refrigerated centrifuge, Hematology analyzer
While so that the Cord blood acquired obtains simply and accurately detecting with screening, detecting step and process are also optimized, is made
Cord blood detection after must acquiring significantly reduces testing cost, not only avoids the wasting of resources, but also subtract more rapidly, efficiently and in time
Detection total time is lacked；The setting of fibre optical sensor ensure that the accuracy of acquisition information；
Wireless routing communication means of the present invention enhances information preservation and locating function, can be realized a little by mobile client
To the data transmission of point without any setting, reach the remote control function of digital display circuit.
Description of the drawings
Fig. 1 is the structural schematic diagram of the detection for cord blood stem cell and screening system provided in an embodiment of the present invention；
In figure：1, collector；2, kit is isolated and purified；3, cell cultivation instrument；4, refrigerated centrifuge；5, whole blood cells point
Analyzer；6, information collection apparatus；7, printing equipment.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.
It please refers to Fig.1：
A kind of detection and screening method for cord blood stem cell include：
Step 1: contained Cord blood in umbilical cord is acquired, dispensed and sampled, and will acquisition information and working condition biography
It send to center control and processing equipment；
Step 2: the cord blood stem cell information to acquisition carries out radio frequency identification RFID pre-configuration；
Step 3: analyzing preconfigured radio frequency identification RFID, the unique mark UID of the RFID is determined, it will
The parameter information of the UID and the carrier of the RFID that determine are combined, and generate anti-counterfeiting information；
Step 4: carrying out stem cell population, competence for added value, micro organism quantity to the cord blood stem cell for generating anti-counterfeiting information
Detection；
Step 5: carrying out epidemiology detection and the detection of HLA tissue matchings to the cord blood stem cell for generating anti-counterfeiting information；
Step 6: the part cell after step 5 and step 5 detection is carried out primary separation, culture amplification, induction
Break up or dedifferente processing, and the cellular informatics that record that treated；
Step 7: to carrying out cell Sterility testing through step 6 treated cell and biological nature detects, it will monitoring letter
Breath is signed according to preconfigured private key, generates anti-counterfeiting information signature；
Step 8: Anti-fake electronic label is written in anti-counterfeiting information signature and the parameter information, interrupt control unit will
Including the Anti-fake electronic label of cellular informatics is uploaded to remote server.
Further, the method for generating anti-counterfeiting information signature is that cipher key store central controller generates random number rkeyid, utilization is ellipse
Circular curve cryptographic algorithm calculates its corresponding public key rPKid, rPKid=rkeyid× G, G are the basic point of elliptic curve, by rPKidWith
User identifier UID is externally sent, and key generation system generates random number rkeyKMC, its phase is calculated using elliptic curve cryptography
The public key rPK answeredKMC, wherein rPKKMC=rkeyKMC× G, and remember γid=rPKid+rPKKMC, specially：
Step 1: calculating identity private key key using user identifier UIDidWith mark public key Rid, specially：
Step 2: generating private key matrix and public key matrix, the size of private key matrix and public key matrix is m × h, m and h equal
For positive integer；
Step 3: the hashed value of user identifier UID is calculated using hashing algorithm using user identifier UID, by the hash
Value is divided into m sections, as private key matrix and public key matrix column mapping value map [i], i=0, and 1,2......m-1；
It calculates：
ri,map[i]For an element in private key matrix；
Xi,map[i]For an element in public key matrix；
N is the rank of elliptic curve.
A kind of detection and screening system for cord blood stem cell, the system include：Collector 1 isolates and purifies reagent
Box 2, cell cultivation instrument 3, refrigerated centrifuge 4, Hematology analyzer 5, information collection apparatus 6, printing equipment 7；
The collector 1 is connect with kit 2 is isolated and purified, and the cell cultivation instrument 3 is isolated and purified with described
Kit 2 connects, and the refrigerated centrifuge 4 is arranged two, is connected respectively at cell cultivation instrument 3, the whole blood cells point
Analyzer 5 is connect with the refrigerated centrifuge 4, and the information collection apparatus 6 is connect with the Hematology analyzer 5,
The printing equipment 7 is connect with the information collection apparatus 6.
Further, the collector 1 uses the closed collection Cord Blood of Neonates of tetrad blood taking bag.
Further, described isolate and purify has separating liquid inside kit 2, separating liquid and bleeding of the umbilicus 4:1 proportional arrangement.
Further, the refrigerated centrifuge 4 divides to prepare centrifuge and work centrifuge.
Further, the full cytoanalyze can detect the packet cell in bleeding of the umbilicus, red blood cell, hemoglobin, lymph
After cell percentages, monocyte percentage and extraction stem cell, mononuclearcell number.
Further, the information collection apparatus 6 uploads the result after full cytoanalyze and stem cell viability examination
To server and it is sent to printing equipment 7；Described information collection device is provided with central control and processing equipment；The collector
Information is acquired using fibre optical sensor；The fibre optical sensor includes that light source generates part, gas chamber, signal control part
Point, light source generate part is made of laser, single mode bidirectional optical fiber coupler, optical splitter, fiber grating, signal control portion by
Photodetector, lock-in amplifier, data collecting card, wavelengt demodulator are constituted, and laser sends out broadband light, and broadband light is through single mode
Fiber grating is reached after bidirectional optical fiber coupler and obtains narrowband emergent light, and narrowband emergent light is divided into two-way through optical splitter, wears all the way
Photodetector in signal control portion is reached after crossing gas chamber, the output signal of photodetector passes after lock-in amplifier amplifies
To data collecting card；Another way obtains centre wavelength value through wavelengt demodulator, and centre wavelength value is adopted by the data of signal control part
Truck acquires.
Data collecting card information collecting method is：
First, echo signal x (t) is acquired within the independent sampling period with awareness apparatus, A/D modes pair is used in combination
Signal carries out digital quantization；Then, dimensionality reduction is carried out to the signal x (i) after quantization；Finally, the signal after dimensionality reduction is reconstructed；
Wherein t is sampling instant, and i is the signal sequence after quantization；
Signal after described pair of quantization carries out dimensionality reduction, specifically passes through finite impulse response filter to the signal after quantization
Difference equation
Wherein h (0) ..., h (L-1) are filter coefficient, design the compressed sensing signal acquisition frame based on filtering, structure
Make following Teoplitz calculation matrix：
Then observeI=1 ..., M, wherein b1,…,bLRegard filter coefficient as；Submatrix ΦFT
Singular value be gram matrix G (ΦF, T) and=Φ 'FTΦFTThe arithmetic root of characteristic value, all eigenvalue λs of verification G (Φ F, T)
i∈(1-δK,1+δK), i=1 ..., T, then ΦFMeet RIP, and by solving following l1Optimization problem reconstructs original signal：
Original signal, that is, BP algorithm are reconstructed by linear programming method；
For actual compression signal Φ is then changed such as the acquisition of voice or picture signalFFor following form：
If signal has sparsity on transformation basic matrix Ψ, by solving following l1Optimization problem, accurate weight
Structure goes out original signal：
Wherein Φ is uncorrelated to Ψ, and Ξ is known as CS matrixes.
Further, described that contained Cord blood in umbilical cord is acquired, dispensed and sampled, and will acquisition information and work shape
State is sent to acquisition information in center control and processing equipment and is sent in center control and processing equipment by wireless routing, nothing
Circuit includes by the communication means communicated：
A, user equipment sends hypertext transfer protocol http request to wireless routing device, obtains certificate server and returns
Portal certification pages redirect response；N number of TD-SCDMA modules, N >=2 are configured in the wireless routing device；
User equipment obtains hot spot signal in the wireless signal coverage area of wireless routing device, carries out network connection；
The wireless routing device issues IP address to the user equipment；The IP address is the source address of the user equipment；Institute
It states user equipment and sends http request to the wireless routing device, the http request includes the source address；It is described wireless
The http request is forwarded to gateway server by routing device；The http request is forwarded to certification by the gateway server
Server；Portal certification pages are redirected response and return to the gateway server by the certificate server；The gateway clothes
Portal certification pages are redirected response and return to the wireless routing device by business device；The wireless routing device recognizes Portal
Card page jump response returns to the user equipment；
B, the user equipment to the wireless routing device send http logging requests, the http logging requests via
Gateway server is forwarded to the certificate server, is authenticated to the http logging requests by the certificate server；
After certification passes through, the certificate server carries out charging to the internet business of the user equipment；
The user equipment sends http logging requests to the wireless routing device；The http logging requests carry
Account and encrypted message；The http logging requests are forwarded to the gateway server by the wireless routing device；The net
It closes server and the http logging requests is forwarded to the certificate server；The certificate server is according to the account and close
Code information, is authenticated the http logging requests；After the authentication has been successful, the certificate server is to the gateway server
Return authentication successful respond, and start to carry out charging to the internet business of the user equipment；
The certification successful respond is returned to the wireless routing device by the gateway server, and records account certification
Information；The account authentication information includes the account and authentication state information of the user equipment；The wireless routing device will
The certification successful respond returns to the user equipment, and records the account authentication information；
C, the user equipment sends network access request to the wireless routing device；The wireless routing device according to
The resource load situation of N number of TD-SCDMA modules, a TD- for selecting resource load minimum by load balancing operation
SCDMA modules provide bandwidth resources for the network access request；The minimum TD-SCDMA modules of the resource load will be described
Network access request is forwarded to the destination address server to be accessed, and the network that the destination address server is returned is visited
Ask that response sends the user equipment to；
The user equipment initiates network access request to the wireless routing device；The network access request includes institute
State source address and the destination address to be accessed；The account authentication information of the wireless routing device inquiry local record, is verified
The account authentication state of the user equipment；If account authentication state is pass flag, the wireless routing device is in
The resource load situation for the N number of TD-SCDMA modules set, a TD- for selecting resource load minimum by load balancing operation
SCDMA modules provide bandwidth resources for the network access request；The minimum TD-SCDMA modules of the resource load will be described
Network access request is forwarded to the destination address server to be accessed；The server of the destination address is to the wireless routing
Equipment returns to network and accesses response；Network access response is returned to the user equipment by the wireless routing device；Institute
It states wireless routing device and sends heartbeat request to the gateway server；The heartbeat request is transmitted to by the gateway server
The certificate server；The certificate server returns to heartbeat response to the gateway server；The gateway server according to
The account authentication information of user equipment described in the content update of the heartbeat response；
D, the user equipment to the wireless routing device send http ask offline, the http offline request via
The gateway server is forwarded to the certificate server, and the certificate server stops the internet business to the user equipment
Carry out charging；
The user equipment sends http to the wireless routing device and asks offline；The wireless routing device will be described
Request is forwarded to the gateway server to http offline；By the http, request is forwarded to and described recognizes the gateway server offline
Demonstrate,prove server；The certificate server carries out processed offline to the user equipment；The certificate server takes to the gateway
Business device returns to offline successful respond, and stops carrying out charging to the internet business of the user equipment；The gateway server will
The offline successful respond returns to the wireless routing device, and deletes the account authentication information of local record；It is described wireless
The offline successful respond is returned to the user equipment by routing device, and deletes the account authentication information of local record.
Further, the printing equipment 7 can print bar code and analysis report.
It after umbilical cord is detached with puerpera and baby, is acquired with harvester, is placed on after the completion of acquisition and isolates and purifies examination
In agent box 2, after standing sedimentation layering in 30 minutes, centrifuge tube is first placed on preparation centrifugation by Aspirate supernatant as in centrifuge tube
In machine, refrigerated centrifuge 4 is centrifuged after five minutes with 2500r/min, abandons supernatant, and single cell suspension is supported with physiological saline, then
Centrifuge tube is placed in work centrifuge, after refrigerated centrifuge 4 is centrifuged 30 minutes with 1800r/min and stem cell is obtained, will do
Cell is put into cell cultivation instrument 3 and carries out cell culture, and cell after culture, which is put into full cytoanalyze, again later carries out cell mirror
It is fixed, analysis result, information collection apparatus 6 by the result after full cytoanalyze and stem cell viability examination be uploaded to server and
It is sent to printing equipment 7, printing equipment 7 can print bar code and analysis report.
The present invention by collector 1, isolate and purify kit 2, cell cultivation instrument 3, refrigerated centrifuge 4, whole blood cells point
While analyzer 5 is so that acquired Cord blood obtains simply and accurately detecting with screening, detecting step and mistake are also optimized
Journey so that the Cord blood detection after acquisition significantly reduces testing cost, both avoids the wasting of resources more rapidly, efficiently and in time,
Reduce detection total time again.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement etc., should all be included in the protection scope of the present invention made by within refreshing and principle.
Claims (8)
1. a kind of detection and screening system for cord blood stem cell, which is characterized in that the system is with the following method to navel
Band hemocytoblast is detected and screening：
Step 1: contained Cord blood in umbilical cord is acquired, dispenses and is sampled, and acquisition information and working condition are sent to
Center control and processing equipment；
Step 2: the cord blood stem cell information to acquisition carries out radio frequency identification RFID pre-configuration；
Step 3: analyzing preconfigured radio frequency identification RFID, determines the unique mark UID of the RFID, will determine
The parameter information of the UID and the carrier of the RFID that go out are combined, and generate anti-counterfeiting information；
It is examined Step 4: carrying out stem cell population, competence for added value, micro organism quantity to the cord blood stem cell for generating anti-counterfeiting information
It surveys；
Step 5: carrying out epidemiology detection and the detection of HLA tissue matchings to the cord blood stem cell for generating anti-counterfeiting information；
Step 6: the part cell after step 5 and step 5 detection is carried out primary separation, culture expands, induction is broken up
Or processing is dedifferented, and record treated cellular informatics；
Step 7: to carrying out cell Sterility testing through step 6 treated cell and biological nature detects, by monitoring information root
It signs according to preconfigured private key, generates anti-counterfeiting information signature；
Step 8: Anti-fake electronic label is written with the parameter information in anti-counterfeiting information signature, interrupt control unit will include
The Anti-fake electronic label of cellular informatics is uploaded to remote server；
Generating the method that anti-counterfeiting information is signed is：Cipher key store central controller generates random number rkeyid, utilize elliptic curve cipher
Algorithm calculates its corresponding public key rPKid, rPKid=rkeyid× G, G are the basic point of elliptic curve, by rPKidAnd user identifier
UID is externally sent, and key generation system generates random number rkeyKMC, its corresponding public key is calculated using elliptic curve cryptography
rPKKMC, wherein rPKKMC=rkeyKMC× G, and remember γid=rPKid+rPKKMC, specially：
A, identity private key key is calculated using user identifier UIDidWith mark public key Rid, specially：
B, it is positive integer that the size of generation private key matrix and public key matrix, private key matrix and public key matrix, which is m × h, m and h,；
C, the hashed value for calculating user identifier UID using hashing algorithm using user identifier UID, is divided into m sections by the hashed value,
As private key matrix and public key matrix column mapping value map [i], i=0,1,2......m-1；
It calculates：
ri,map[i]For an element in private key matrix；
Xi,map[i]For an element in public key matrix；
N is the rank of elliptic curve；
The system includes：Collector isolates and purifies kit, cell cultivation instrument, refrigerated centrifuge, Hematology analyzer, letter
Cease collection device, printing equipment；
The collector is connect with kit is isolated and purified, and the cell cultivation instrument isolates and purifies kit company with described
Connect, the refrigerated centrifuge be arranged two, respectively at cell cultivation instrument connect, the Hematology analyzer with it is described
Refrigerated centrifuge connects, and the information collection apparatus connect with the Hematology analyzer, the printing equipment and
The information collection apparatus connection.
2. as described in claim 1 for the detection of cord blood stem cell and screening system, which is characterized in that the separation
There are separating liquid, separating liquid and bleeding of the umbilicus 4 inside purification kit:1 proportional arrangement.
3. as described in claim 1 for the detection of cord blood stem cell and screening system, which is characterized in that the low temperature
Centrifuge, which is divided into, prepares centrifuge and work centrifuge.
4. as described in claim 1 for the detection of cord blood stem cell and screening system, which is characterized in that the whole blood
Cytoanalyze can detect the packet cell in bleeding of the umbilicus, red blood cell, hemoglobin, cent lymphocytes, monocyte percentage
Than and extraction stem cell after, mononuclearcell number.
5. as described in claim 1 for the detection of cord blood stem cell and screening system, which is characterized in that the information
Result after Hematology analyzer and stem cell viability examination is uploaded to server and is sent to printing equipment by collection device,
Described information collection device is provided with central control and processing equipment；
The collector, which is provided with, is acquired information using fibre optical sensor；The fibre optical sensor includes light source generating unit
Divide, gas chamber, signal control portion, light source generates part by laser, single mode bidirectional optical fiber coupler, optical splitter, fiber grating
It constitutes, signal control portion is made of photodetector, lock-in amplifier, data collecting card, wavelengt demodulator, and laser is sent out
Broadband light, broadband light reach fiber grating after single mode bidirectional optical fiber coupler and obtain narrowband emergent light, and narrowband emergent light is through dividing
Light device is divided into two-way, and photodetector in signal control portion, the output signal of photodetector are reached after passing through gas chamber all the way
Data collecting card is reached after lock-in amplifier amplifies；Another way obtains centre wavelength value, centre wavelength value through wavelengt demodulator
It is acquired by the data collecting card of signal control part.
6. as described in claim 1 for the detection of cord blood stem cell and screening system, which is characterized in that data collecting card
Information collecting method is：
First, echo signal x (t) is acquired within the independent sampling period with awareness apparatus, is used in combination A/D modes to signal
Carry out digital quantization；Then, dimensionality reduction is carried out to the signal x (i) after quantization；Finally, the signal after dimensionality reduction is reconstructed；Wherein
T is sampling instant, and i is the signal sequence after quantization；
Signal after described pair of quantization carries out dimensionality reduction, the difference for specifically passing through finite impulse response filter to the signal after quantization
Divide equation
Wherein h (0) ..., h (L-1) are filter coefficient, design the compressed sensing signal acquisition frame based on filtering,
Construct following Teoplitz calculation matrix：
Then observeWherein b1,…,bLRegard filter coefficient as；Submatrix ΦFTIt is strange
Different value is gram matrix G (ΦF, T) and=Φ 'FTΦFTThe arithmetic root of characteristic value, all eigenvalue λ i ∈ of verification G (Φ F, T)
(1-δK,1+δK), i=1 ..., T, then ΦFMeet RIP, and by solving following l1Optimization problem reconstructs original signal：
Original signal, that is, BP algorithm are reconstructed by linear programming method；
For actual compression signal Φ is then changed such as the acquisition of voice or picture signalFFor following form：
If signal has sparsity on transformation basic matrix Ψ, by solving following l1Optimization problem, Accurate Reconstruction go out original
Signal：
Wherein Φ is uncorrelated to Ψ, and Ξ is known as CS matrixes.
7. as described in claim 1 for the detection of cord blood stem cell and screening system, which is characterized in that described in umbilical cord
Contained Cord blood is acquired, dispenses and samples, and acquisition information and working condition are sent to central control and processing equipment
Middle acquisition information is sent to by wireless routing in center control and processing equipment, and the communication means of wireless routing communication includes：
A, user equipment sends hypertext transfer protocol http request to wireless routing device, obtains what certificate server returned
Portal certification pages redirect response；N number of TD-SCDMA modules, N >=2 are configured in the wireless routing device；
User equipment obtains hot spot signal in the wireless signal coverage area of wireless routing device, carries out network connection；It is described
Wireless routing device issues IP address to the user equipment；The IP address is the source address of the user equipment；The use
Family equipment sends http request to the wireless routing device, and the http request includes the source address；The wireless routing
The http request is forwarded to gateway server by equipment；The http request is forwarded to authentication service by the gateway server
Device；Portal certification pages are redirected response and return to the gateway server by the certificate server；The gateway server
Portal certification pages are redirected into response and return to the wireless routing device；The wireless routing device is by Portal authentication pages
Face redirects response and returns to the user equipment；
B, the user equipment sends http logging requests to the wireless routing device, and the http logging requests are via gateway
Server is forwarded to the certificate server, is authenticated to the http logging requests by the certificate server；In certification
By rear, the certificate server carries out charging to the internet business of the user equipment；
The user equipment sends http logging requests to the wireless routing device；The http logging requests carry account
And encrypted message；The http logging requests are forwarded to the gateway server by the wireless routing device；The gateway clothes
The http logging requests are forwarded to the certificate server by business device；The certificate server is according to the account and message in cipher
Breath, is authenticated the http logging requests；After the authentication has been successful, the certificate server is returned to the gateway server
Certification successful respond, and start to carry out charging to the internet business of the user equipment；
The certification successful respond is returned to the wireless routing device by the gateway server, and records account certification letter
Breath；The account authentication information includes the account and authentication state information of the user equipment；The wireless routing device is by institute
It states certification successful respond and returns to the user equipment, and record the account authentication information；
C, the user equipment sends network access request to the wireless routing device；The wireless routing device is according to
The resource load situation of N number of TD-SCDMA modules, a TD-SCDMA for selecting resource load minimum by load balancing operation
Module provides bandwidth resources for the network access request；The minimum TD-SCDMA modules of the resource load visit the network
Ask that request is forwarded to the destination address server to be accessed, and the network that the destination address server is returned accesses response
Send the user equipment to；
The user equipment initiates network access request to the wireless routing device；The network access request includes the source
Address and the destination address to be accessed；The account authentication information of wireless routing device inquiry local record, described in verification
The account authentication state of user equipment；If account authentication state is pass flag, the wireless routing device is according to built-in N
The resource load situation of a TD-SCDMA modules, a TD-SCDMA mould for selecting resource load minimum by load balancing operation
Block provides bandwidth resources for the network access request；The minimum TD-SCDMA modules of the resource load access the network
Request is forwarded to the destination address server to be accessed；The server of the destination address is returned to the wireless routing device
Network accesses response；Network access response is returned to the user equipment by the wireless routing device；The no circuit
Heartbeat request is sent from equipment to the gateway server；The heartbeat request is transmitted to the certification by the gateway server
Server；The certificate server returns to heartbeat response to the gateway server；The gateway server is according to the heartbeat
The account authentication information of user equipment described in the content update of response；
D, the user equipment sends http to the wireless routing device and asks offline, and the http is asked via described offline
Gateway server is forwarded to the certificate server, and the certificate server stops carrying out the internet business of the user equipment
Charging；
The user equipment sends http to the wireless routing device and asks offline；The wireless routing device is by the http
Offline request is forwarded to the gateway server；By the http, request is forwarded to the certification clothes to the gateway server offline
Business device；The certificate server carries out processed offline to the user equipment；The certificate server is to the gateway server
Offline successful respond is returned, and stops carrying out charging to the internet business of the user equipment；The gateway server will be described
Offline successful respond returns to the wireless routing device, and deletes the account authentication information of local record；The wireless routing
The offline successful respond is returned to the user equipment by equipment, and deletes the account authentication information of local record.
8. as described in claim 1 for the detection of cord blood stem cell and screening system, which is characterized in that the printing
Device can print bar code and analysis report.
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| CN109287617A (en) * | 2018-10-23 | 2019-02-01 | 广东省心血管病研究所 | A kind of cord blood stem cell preservation system |
| KR102030785B1 (en) * | 2019-04-26 | 2019-10-10 | 주식회사그린존시큐리티 | An apparatus for obfuscating data of IoT devices using pseudorandom number and a method therefor |
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| CN201673518U (en) * | 2010-05-28 | 2010-12-15 | 绍兴怡和信息***技术有限公司 | Blood bank management system based on RFID |
| CN105190638A (en) * | 2013-03-14 | 2015-12-23 | 柯惠有限合伙公司 | Rfid secure authentication |
| CN105303211A (en) * | 2015-11-09 | 2016-02-03 | 秦卫东 | DNA blood sample library creating system and method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201673518U (en) * | 2010-05-28 | 2010-12-15 | 绍兴怡和信息***技术有限公司 | Blood bank management system based on RFID |
| CN105190638A (en) * | 2013-03-14 | 2015-12-23 | 柯惠有限合伙公司 | Rfid secure authentication |
| CN105303211A (en) * | 2015-11-09 | 2016-02-03 | 秦卫东 | DNA blood sample library creating system and method |
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