CN106349315A - Momordica grosvenori extraction method - Google Patents
Momordica grosvenori extraction method Download PDFInfo
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- CN106349315A CN106349315A CN201510411312.6A CN201510411312A CN106349315A CN 106349315 A CN106349315 A CN 106349315A CN 201510411312 A CN201510411312 A CN 201510411312A CN 106349315 A CN106349315 A CN 106349315A
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- specific density
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- momordica grosvenori
- acetone
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- 238000000605 extraction Methods 0.000 title abstract description 10
- 241001409321 Siraitia grosvenorii Species 0.000 title abstract 6
- 235000011171 Thladiantha grosvenorii Nutrition 0.000 title abstract 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 26
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000004382 Amylase Substances 0.000 claims abstract description 10
- 108010065511 Amylases Proteins 0.000 claims abstract description 10
- 102000013142 Amylases Human genes 0.000 claims abstract description 10
- 235000019418 amylase Nutrition 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000001816 cooling Methods 0.000 claims abstract description 5
- 238000009928 pasteurization Methods 0.000 claims abstract description 5
- 238000012216 screening Methods 0.000 claims abstract description 5
- 230000008961 swelling Effects 0.000 claims abstract description 5
- 239000000843 powder Substances 0.000 claims abstract description 3
- 235000015203 fruit juice Nutrition 0.000 claims description 12
- 108010059892 Cellulase Proteins 0.000 claims description 8
- 229940106157 cellulase Drugs 0.000 claims description 8
- 235000013399 edible fruits Nutrition 0.000 claims description 7
- 239000000284 extract Substances 0.000 claims description 6
- 238000001556 precipitation Methods 0.000 claims description 6
- -1 add pectase 3ml Proteins 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- 230000001376 precipitating effect Effects 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 239000012141 concentrate Substances 0.000 claims description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 abstract 3
- GHBNZZJYBXQAHG-KUVSNLSMSA-N (2r,3r,4s,5s,6r)-2-[[(2r,3s,4s,5r,6r)-6-[[(3s,8s,9r,10r,11r,13r,14s,17r)-17-[(2r,5r)-5-[(2s,3r,4s,5s,6r)-4,5-dihydroxy-3-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-[[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@H](CC[C@@H](C)[C@@H]1[C@]2(C[C@@H](O)[C@@]3(C)[C@H]4C(C([C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]6[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O6)O)O5)O)CC4)(C)C)=CC[C@H]3[C@]2(C)CC1)C)C(C)(C)O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O GHBNZZJYBXQAHG-KUVSNLSMSA-N 0.000 abstract 2
- 108010059820 Polygalacturonase Proteins 0.000 abstract 2
- 229920002678 cellulose Polymers 0.000 abstract 2
- 239000001913 cellulose Substances 0.000 abstract 2
- 108010093305 exopolygalacturonase Proteins 0.000 abstract 2
- TVJXHJAWHUMLLG-UHFFFAOYSA-N mogroside V Natural products CC(CCC(OC1OC(COC2OC(CO)C(O)C(O)C2OC3OC(CO)C(O)C(O)C3O)C(O)C(O)C1O)C(C)(C)O)C4CCC5(C)C6CC=C7C(CCC(OC8OC(COC9OC(CO)C(O)C(O)C9O)C(O)C(O)C8O)C7(C)C)C6(C)C(O)CC45C TVJXHJAWHUMLLG-UHFFFAOYSA-N 0.000 abstract 2
- 239000002244 precipitate Substances 0.000 abstract 2
- 238000009835 boiling Methods 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 230000003068 static effect Effects 0.000 abstract 1
- TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 description 12
- PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 description 12
- PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 description 12
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 3
- 229930195725 Mannitol Natural products 0.000 description 3
- 235000010355 mannitol Nutrition 0.000 description 3
- 239000000594 mannitol Substances 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 230000000954 anitussive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Seasonings (AREA)
- Jellies, Jams, And Syrups (AREA)
- Medicines Containing Plant Substances (AREA)
- Non-Alcoholic Beverages (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention provides a momordica grosvenori extraction method which is characterized by comprising the following steps: (1) screening fresh momordica grosvenori or dry momordica grosvenori, swelling the momordica grosvenori, spirally squeezing the momordica grosvenori to obtain juice, performing pasteurization, then adding pectinase, amylase and cellulose, adding 3 ml of the pectinase, 6 ml of the amylase and 8 ml of the cellulose into each liter of juice, and preserving the heat at the temperature of 30 to 40 DEG C for 2 hours for enzymolysis; (2) concentrating the juice obtained in the step (1) in a vacuum concentrator until the specific density is 1.2; (3) adding 90 to 95 percent ethanol to enable the alcohol content to be 65%, conducting static settlement, extracting a supernate, and concentrating to be free of alcohol taste until the specific density is 1.2; (4) adding pure water into the liquid obtained in the step (3), adding a proper amount of activated carbon, boiling for 20 minutes, cooling and filtering the liquid to obtain water clarified effluent, and concentrating the effluent until the specific density is 1.2; (5) adding acetone into the liquid obtained in the step (4), enabling the acetone concentration (V/V) to be 50 to 75 percent, and separating out precipitates; (6) adding pure water into the precipitates obtained in the step (5), performing high-vacuum concentration to remove the acetone to obtain paste, and finally drying the paste to obtain powder. By the adoption of the method for extracting the mogroside V, the extracted mogroside V is high in yield, high in purity and high in quality.
Description
Technical field
The present invention relates to a kind of Fructus Momordicae extracting method.
Background technology
At present to Fructus Momordicae using being mainly used in extracting Momordia grosvenori aglycone v, Momordia grosvenori aglycone v is used for sweeting agent, and the Mannitol being used for antitussive action is not utilized well.Momordia grosvenori aglycone v content highest in Lo Han Guo fruit, and Momordia grosvenori aglycone v and Mannitol also have certain content in fruit, draw materials conveniently, widely distributed, low price.Thus, carrying out comprehensive deep processing to Fructus Momordicae means a great, and explores and extracts Momordia grosvenori aglycone v and Mannitol from Lo Han Guo fruit simultaneously, and its meaning is quite long-range.
But the extraction process of current Momordia grosvenori aglycone v is complicated, and the purity of obtained Momordia grosvenori aglycone v is not high.
Content of the invention
The present invention proposes a kind of Fructus Momordicae extracting method, this extracting method process is simple, extracts Momordia grosvenori aglycone v purity height, can reach more than 70%, and quality is good.
The technical scheme is that and be achieved in that: a kind of Fructus Momordicae extraction process, comprise the steps:
(1) screening fresh Fructus Momordicae or dry fruit swelling, screw press goes out fruit juice, and carry out pasteurization, it is subsequently adding pectase, amylase and cellulase, pectase 3ml, amylase 6ml, cellulase 8ml is added in every liter of fruit juice liquid, it is incubated at 30-40 DEG C and is digested for 2 hours, the activity of wherein enzyme is 20u/mg；
(2) fruit juice obtaining step (1) is concentrated into specific density in vacuum concentrator is 1.2；
(3) adding 90%-95% ethanol to make alcohol content is 65%, staticly settles, and extracts supernatant, is concentrated into no alcohol taste, and specific density is 1.2；
(4) liquid that step (3) obtains is added pure water, and add proper amount of active carbon, boil 20min, cooling down simultaneously filters, and obtains water precipitating clear liquid, being concentrated into specific density is 1.2.
(5) add acetone in the liquid that step (4) obtains, make acetone concentration (v/v) reach 50-75%, separate out precipitation；
(6) precipitation that step (5) obtains is added pure water, and carry out fine vacuum and concentrate to remove acetone, obtain stiff paste, be finally dried and obtain powder.
The invention has the beneficial effects as follows: carry out the extraction of Momordia grosvenori aglycone v using the method, the Momordia grosvenori aglycone v purity of extraction is high, and quality is good.
Specific embodiment
Technical scheme in the embodiment of the present invention will be clearly and completely described below it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained under the premise of not making creative work, broadly fall into the scope of protection of the invention.
Embodiment 1:
A kind of Fructus Momordicae extraction process, comprises the steps:
(1) screening fresh Fructus Momordicae or dry fruit swelling, screw press goes out fruit juice, and carry out pasteurization, it is subsequently adding pectase, amylase and cellulase, pectase 3ml, amylase 6ml, cellulase 8ml is added in every liter of fruit juice liquid, it is incubated at 30-40 DEG C and is digested for 2 hours, the activity of wherein enzyme is 20u/mg；
(2) fruit juice that step one obtains being concentrated in vacuum concentrator specific density is 1.2；
(3) adding 90% ethanol to make alcohol content is 65%, staticly settles, and extracts supernatant, is concentrated into no alcohol taste, and specific density is 1.2；
(4) liquid that step (3) obtains is added pure water, and add proper amount of active carbon, boil 20min, cooling down simultaneously filters, and obtains water precipitating clear liquid, being concentrated into specific density is 1.2
(5) add acetone in the liquid that step (4) obtains, make acetone concentration (v/v) reach 75%, separate out precipitation.
Embodiment 1:
A kind of Fructus Momordicae extraction process, comprises the steps:
(1) screening fresh Fructus Momordicae or dry fruit swelling, screw press goes out fruit juice, and carries out pasteurization, is subsequently adding pectase, amylase and cellulase, add pectase 3ml, amylase 6ml, cellulase 8ml in every liter of fruit juice liquid, be incubated at 30-40 DEG C and digested for 2 hours；
(2) fruit juice that step one obtains being concentrated in vacuum concentrator specific density is 1.2；
(3) adding 90% ethanol to make alcohol content is 65%, staticly settles, and extracts supernatant, is concentrated into no alcohol taste, and specific density is 1.2；
(4) liquid that step (3) obtains is added pure water, and add proper amount of active carbon, boil 20min, cooling down simultaneously filters, and obtains water precipitating clear liquid, being concentrated into specific density is 1.2
(5) add acetone in the liquid that step (4) obtains, make acetone concentration (v/v) reach 65%, separate out precipitation.
Carry out the extraction of Momordia grosvenori aglycone v using the method, the Momordia grosvenori aglycone v purity of extraction is high, and quality is good.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all any modification, equivalent substitution and improvement within the spirit and principles in the present invention, made etc., should be included within the scope of the present invention.
Claims (1)
1. a kind of Fructus Momordicae extracting method is it is characterised in that comprise the steps:
(1) screening fresh Fructus Momordicae or dry fruit swelling, screw press goes out fruit juice, and carries out pasteurization, is subsequently adding pectase, amylase and cellulase, add pectase 3ml, amylase 6ml, cellulase 8ml in every liter of fruit juice liquid, be incubated at 30-40 DEG C and digested for 2 hours；
(2) fruit juice obtaining step (1) is concentrated into specific density in vacuum concentrator is 1.2；
(3) adding 90%-95% ethanol to make alcohol content is 65%, staticly settles, and extracts supernatant, is concentrated into no alcohol taste, and specific density is 1.15；
(4) liquid that step (3) obtains is added pure water, and add proper amount of active carbon, boil 20min, cooling down simultaneously filters, and obtains water precipitating clear liquid, being concentrated into specific density is 1.2
(5) add acetone in the liquid that step (4) obtains, make acetone concentration (v/v) reach 50-75%, separate out precipitation；
(6) precipitation that step (5) obtains is added pure water, and carry out fine vacuum and concentrate to remove acetone, obtain stiff paste, be finally dried and obtain powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510411312.6A CN106349315B (en) | 2015-07-14 | 2015-07-14 | A kind of Momordica grosvenori extracting method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510411312.6A CN106349315B (en) | 2015-07-14 | 2015-07-14 | A kind of Momordica grosvenori extracting method |
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| Publication Number | Publication Date |
|---|---|
| CN106349315A true CN106349315A (en) | 2017-01-25 |
| CN106349315B CN106349315B (en) | 2017-12-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510411312.6A Active CN106349315B (en) | 2015-07-14 | 2015-07-14 | A kind of Momordica grosvenori extracting method |
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| Country | Link |
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| CN (1) | CN106349315B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108433054A (en) * | 2018-03-09 | 2018-08-24 | 合浦穗香食品有限公司 | A kind of pastry food additive and application |
| CN108635442A (en) * | 2018-05-18 | 2018-10-12 | 双柏燚天商贸有限公司 | A kind of finger citron sliced formula and processing technology |
| CN108651662A (en) * | 2018-05-18 | 2018-10-16 | 双柏燚天商贸有限公司 | A kind of fingered citron instant tea formula and processing technology |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101200753A (en) * | 2007-08-09 | 2008-06-18 | 桂林惠通生物科技有限公司 | Fructus momordicae extract with mogroside V content being more than or equal to 40% and preparation method thereof |
| CN102675398A (en) * | 2012-05-27 | 2012-09-19 | 唐忠海 | Method for extracting momordica grosvenori glycoside V and farnesol from grosvenor momordica fruits |
| CN102742906A (en) * | 2012-07-18 | 2012-10-24 | 何伟平 | Momordica grosvenori drink with low glycemic index and anti-fatigue action |
-
2015
- 2015-07-14 CN CN201510411312.6A patent/CN106349315B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101200753A (en) * | 2007-08-09 | 2008-06-18 | 桂林惠通生物科技有限公司 | Fructus momordicae extract with mogroside V content being more than or equal to 40% and preparation method thereof |
| CN102675398A (en) * | 2012-05-27 | 2012-09-19 | 唐忠海 | Method for extracting momordica grosvenori glycoside V and farnesol from grosvenor momordica fruits |
| CN102742906A (en) * | 2012-07-18 | 2012-10-24 | 何伟平 | Momordica grosvenori drink with low glycemic index and anti-fatigue action |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108433054A (en) * | 2018-03-09 | 2018-08-24 | 合浦穗香食品有限公司 | A kind of pastry food additive and application |
| CN108635442A (en) * | 2018-05-18 | 2018-10-12 | 双柏燚天商贸有限公司 | A kind of finger citron sliced formula and processing technology |
| CN108651662A (en) * | 2018-05-18 | 2018-10-16 | 双柏燚天商贸有限公司 | A kind of fingered citron instant tea formula and processing technology |
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| Publication number | Publication date |
|---|---|
| CN106349315B (en) | 2017-12-01 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: An extraction method of Siraitia grosvenorii Effective date of registration: 20201207 Granted publication date: 20171201 Pledgee: Xiushan Industrial Development Investment Co.,Ltd. Pledgor: CHONGQING WANWU CHUNSHENG PHARMACEUTICAL Co.,Ltd. Registration number: Y2020500000022 |
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Date of cancellation: 20220222 Granted publication date: 20171201 Pledgee: Xiushan Industrial Development Investment Co.,Ltd. Pledgor: CHONGQING WANWU CHUNSHENG PHARMACEUTICAL Co.,Ltd. Registration number: Y2020500000022 |
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Effective date of registration: 20220309 Address after: 409900 No. 6, Wuyang Avenue, Wuyang street, Qianjiang District, Chongqing Patentee after: CHONGQING GUOTAI KANGNING PHARMACEUTICAL Co.,Ltd. Address before: 409900 LU1, park, Wuyang street, Xiushan Tujia and Miao Autonomous County, Chongqing Patentee before: CHONGQING WANWU CHUNSHENG PHARMACEUTICAL Co.,Ltd. |
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Address after: 409900 No. 6, Wuyang Avenue, Wuyang street, Xiushan County, Chongqing Patentee after: CHONGQING GUOTAI KANGNING PHARMACEUTICAL Co.,Ltd. Address before: 409900 No. 6, Wuyang Avenue, Wuyang street, Qianjiang District, Chongqing Patentee before: CHONGQING GUOTAI KANGNING PHARMACEUTICAL Co.,Ltd. |