CN106338574B - A kind of artificial dirt and test piece of defiling - Google Patents
A kind of artificial dirt and test piece of defiling Download PDFInfo
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- CN106338574B CN106338574B CN201510393870.4A CN201510393870A CN106338574B CN 106338574 B CN106338574 B CN 106338574B CN 201510393870 A CN201510393870 A CN 201510393870A CN 106338574 B CN106338574 B CN 106338574B
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- artificial dirt
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Abstract
The present invention relates to a kind of artificial dirts and test piece of defiling, the artificial dirt is made of albumin, immunoglobulin, protamine, ferrodoxins, cholesterol, triglycerides, oleic acid, phosphatide, bilirubin, Photosensitive surfactant and crosslinking agent, above-mentioned composition is dissolved, it stirs evenly, obtain artificial dirt, the dirt being commonly encountered in the artificial dirt analog Biochemical Analyzer detection;The test piece of defiling is to be coated with the test piece of above-mentioned artificial dirt to form through aging process, and the test piece absorbance variation of defiling is small, can be used to the cleaning performance for evaluating biochemical cleaning solution.
Description
Technical field
The invention belongs to a kind of artificial dirts and test piece of defiling, when specifically one kind is similar to using Biochemical Analyzer
One kind of the dirt and the detergency for detecting Biochemical Analyzer cleaning solution that encounter is defiled test piece.
Background technique
The unusual daily cleaning solution of biochemical cleaning solution and industry cleaning link liquid, should have wetting power is strong, cleaning effect is good, point
The features such as dissipating function admirable.However direct cleaning is mostly used there is no the respective standard for evaluating biochemical cleaning solution both at home and abroad at present
Biochemical Analyzer judges cleaning effect by comparing the method for repeated detection result collimation.This method not only needs to examine
Multiple projects are surveyed, also need to measure a large amount of sample, and the unstability of sample, it is unreliable to may cause testing result.In view of clear
It washes effect and directly determines detection effect, therefore be highly desirable to formulate the test method of biochemical cleaning solution cleaning effect.In order to make
The test method is accurate and effective, it is therefore necessary to develop it is a kind of can true simulation using common in Biochemical Analyzer, hold
Easily cause the artificial dirt of cross contamination.Dirt is mainly derived from sample (whole blood, blood plasma or serum) in biochemical analysis, followed by
Reagent is finally the dust fallen into.The project that cross contamination often occurs in clinical examination has triglycerides, cholesterol, gallbladder red
Element, lipoprotein etc..Therefore dirt may include albumen, lipid and insoluble solid.Prepared artificial dirt and practical dirt
Want similar, the effect cleaned to it can be reflected in the cleaning ability for dividing in instrument using biochemistry and encountering dirt.Dirt is in test piece of defiling
Upper to clean by cleaning solution, removal falls off uniformly, and data reproduction and collimation will be got well, the quantitative reflection cleaning effect of energy.And
It does not find also to meet above-mentioned requirements artificial dirt at present.
Summary of the invention
The purpose of the present invention is to provide a kind of artificial dirt and the preparation methods for test piece of defiling, and are prepared by this method
The running practical dirt of artificial dirt analog Biochemical Analyzer, test piece of defiling are used to carry out the performance evaluation of cleaning solution, and
And the artificial dirt in test piece of defiling falls off uniformly when cleaned, can more true, objectively reflect biochemical cleaning solution
Cleaning performance.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of artificial dirt contains the mass ratio of various components in the dirt are as follows:
Albumin 10-50
Immunoglobulin 1-20
Protamine 1-5
Ferrodoxins 1-5
Cholesterol 1-20
Triglycerides 1-10
Oleic acid 1-10
Phosphatidase 0 .01-10
Bilirubin 0.01-5
Photosensitive surfactant is appropriate
Appropriate crosslinking agent
It is another object of the present invention to what is be achieved through the following technical solutions:
The preparation step of above-mentioned artificial dirt is as follows:
A. the albumin, immunoglobulin, protamine, ferredoxin, bilirubin is weighed by said ratio to use
Deionized water dissolving, and appropriate above-mentioned surfactant is added and obtains solution 1, by cholesterol, phosphatide, triglycerides, oleic acid second
Alcohol dissolves to obtain solution 2, room temperature or 37 DEG C of stirrings, and solution 2 is injected into solution 1, so that it is uniformly dispersed, obtains homogeneous latex emulsion;
B. crosslinking agent is added into above-mentioned emulsion, stirs evenly, obtains artificial dirt;
The preparation step of above-mentioned test piece is as follows:
A. test piece is cleaned, carries out drying and processing and cooling treatment, measures absorbance later, the test piece can be quartz
Glass, hard glass, simple glass and organic glass;
B. the artificial dirt is smeared in the test piece, obtains the test piece for being coated with artificial dirt;Measurement is inhaled later
Luminosity, the absorbance variation CV between test piece of defiling are not more than 10%, CV of each test piece in the absorbance difference for front and back of defiling
No more than 10%;
C. by the test piece for being coated with artificial dirt, left at room temperature over night, so as to the crosslinking of albumen and lipid;
D. it carries out the artificial dirt test piece to be dried under reduced pressure processing, carries out ultraviolet light irradiation during this period three times;?
In the irradiation of ultraviolet light three times, irradiation ultraviolet wavelength used is between 200-365nm.
Specific embodiment:
Embodiment 1:
A kind of artificial dirt, the weight (g) in the dirt containing each component compare are as follows:
Albumin 5
Immunoglobulins 2
Protamine 0.5
Ferrodoxins 0.5
Cholesterol 0.2
Phosphatidase 0 .5
Triglycerides 3
Oleic acid 0.01
Ethyl alcohol 30
Bilirubin 0.01
Azobenzene surfactant 10
EDAC 0.1
Embodiment 2:
A kind of preparation method of artificial dirt, preparation step are as follows:
The albumin, immunoglobulin, essence are weighed by the quality proportioning of above-mentioned component in the beaker that specification is 100mL
Albumen, ferrodoxins, bilirubin are added deionized water dissolving, add azobenzene surfactant, obtain solution 1;
Cholesterol, phosphatide, triglycerides, oleic acid are weighed, is dissolved with ethyl alcohol, solution 1 is added while stirring, stirs evenly, is obtained artificial
Dirt.
Embodiment 3:
A kind of preparation for test piece of defiling, preparation step are as follows:
The shape of the test piece can be cuboid, lower cube, and material is simple glass, it is characterised in that test piece extinction
The CV of degree is not more than 10%.The artificial dirt is smeared in the test piece of clean dry, obtains the test piece for being coated with artificial dirt,
It is characterized in that the CV of test piece absorbance no more than 10%;In above-mentioned smearing, using spreader uniformly by artificial dirt brushing
At the middle part of test piece single side;And it is No. 1-24 that this, which is coated with test piece number of artificial dirt,;
The test piece for being coated with artificial dirt is subjected to cross-linking reaction, described is specially coated with being placed on for artificial dirt
On dry porcelain plate, it is put into 37 DEG C of drying boxes overnight;
The artificial dirt test piece is dried under reduced pressure and ultraviolet treatment with irradiation.The temperature of drying box is 45 DEG C,
Drying time is 4.5 hours, is irradiated respectively with the ultraviolet light of wavelength 254nm, 312nm and 365nm during drying, each wavelength is purple
The irradiation time of outside line is 1.5 hours.
Claims (6)
1. a kind of artificial dirt, it is characterised in that: the artificial dirt is aoxidized by albumin, immunoglobulin, protamine, iron
Restore albumen, cholesterol, phosphatide, triglycerides, oleic acid, bilirubin, surfactant and crosslinking agent composition, above each raw material weight
Measure portion rate are as follows:
2. a kind of preparation method of artificial dirt as described in claim 1, it is characterised in that:
A. by said ratio weigh the albumin, immunoglobulin, protamine, ferredoxin, bilirubin spend from
Sub- water dissolution, and appropriate above-mentioned surfactant is added and obtains solution 1, cholesterol, phosphatide, triglycerides, oleic acid ethyl alcohol is molten
Solution 2, room temperature or 37 DEG C of stirrings are solved, solution 2 is injected into solution 1, so that it is uniformly dispersed, obtains homogeneous microemulsion liquid;
B. crosslinking agent is added into above-mentioned emulsion, stirs evenly, obtains artificial dirt.
3. a kind of preparation method for test piece of defiling, it is characterised in that:
A. test piece prepares: test piece being cleaned, drying and processing and cooling treatment is carried out, surveys initial absorbance later, it is ensured that absorbance
CV be not more than 10%;
B. artificial dirt described in claim 1 is smeared in the test piece, obtains the test piece for being coated with artificial dirt;Respectively it is coated with
The CV of the absorbance of the test piece of artificial dirt is not more than 10%;
C. the test piece for being coated with artificial dirt described in B is carried out being dried under reduced pressure processing, obtains artificial dirt test piece;
D. the artificial dirt test piece is subjected to ultraviolet light irradiation three times again;Obtain the artificial dirt test piece of aging.
4. the preparation method of test piece according to claim 3 of defiling, it is characterised in that: irradiated in the ultraviolet light three times
In, while being irradiated with the ultraviolet light that wavelength is respectively 254nm, 312nm, 365nm.
5. the preparation method of test piece according to claim 3 of defiling, it is characterised in that: the absorbance CV between test piece of defiling
No more than 10%, each test piece is not more than 10% in the CV of the absorbance difference for front and back of defiling.
6. the preparation method of test piece according to claim 3 of defiling, it is characterised in that: the test piece can be used for biochemical cleaning
The performance evaluation of agent.
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CN201510393870.4A CN106338574B (en) | 2015-07-07 | 2015-07-07 | A kind of artificial dirt and test piece of defiling |
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CN201510393870.4A CN106338574B (en) | 2015-07-07 | 2015-07-07 | A kind of artificial dirt and test piece of defiling |
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CN106338574B true CN106338574B (en) | 2019-02-15 |
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CN111044337B (en) * | 2019-12-20 | 2022-07-12 | 广东好顺欧迪斯科技股份有限公司 | Artificial oil stain and preparation method and application thereof |
CN114062416A (en) * | 2021-12-03 | 2022-02-18 | 应急管理部天津消防研究所 | Smoke standard dirty cloth and method for evaluating decontamination performance of fire-extinguishing protective clothing product |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1225676A (en) * | 1996-05-17 | 1999-08-11 | 普罗格特-甘布尔公司 | Detergent composition |
CN102942998A (en) * | 2012-10-30 | 2013-02-27 | 湖南日用化学科学研究所有限公司 | Washing auxiliary agent for removing protein stains |
CN103197037A (en) * | 2013-04-28 | 2013-07-10 | 北京绿伞化学股份有限公司 | Artificial dirt, test piece, and preparation method for artificial dirt |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH115997A (en) * | 1997-06-18 | 1999-01-12 | Lion Corp | Liquid detergent composition |
US9125839B2 (en) * | 2010-03-12 | 2015-09-08 | The Nisshin Oillio Group, Ltd. | Composition for external use on skin, cosmetic, and cleaning agent |
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2015
- 2015-07-07 CN CN201510393870.4A patent/CN106338574B/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1225676A (en) * | 1996-05-17 | 1999-08-11 | 普罗格特-甘布尔公司 | Detergent composition |
CN102942998A (en) * | 2012-10-30 | 2013-02-27 | 湖南日用化学科学研究所有限公司 | Washing auxiliary agent for removing protein stains |
CN103197037A (en) * | 2013-04-28 | 2013-07-10 | 北京绿伞化学股份有限公司 | Artificial dirt, test piece, and preparation method for artificial dirt |
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