CN106337055A - Application of nitrate radical transporter gene OsNRT1.8 in rice breeding - Google Patents
Application of nitrate radical transporter gene OsNRT1.8 in rice breeding Download PDFInfo
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Abstract
The invention discloses application of a nitrate radical transporter gene OsNRT1.8 in rice breeding and belongs to the field of plant genetic engineering. The protein encoding amino acid sequence of the gene OsNRT1.8 is shown as SEQ ID NO.1, and a cDNA sequence is shown as SEQ ID NO.2. By establishing rice gene OsNRT1.8 overexpression plant and a gene OsNRT1.8 interference plant, it is found that normal rice tiller number and spike number of each plant can be increased by improving gene OsNRT1.8 expression. Therefore, the gene OsNRT1.8 can be used for increasing rice yield in rice breeding. The gene OsNRT1.8 has an important application value on the aspects of elaboration about nitrogen influence on plant growth and development and improvement of rice plant type.
Description
Technical field
The invention belongs to plant genetic engineering field is and in particular to nitrate anion transporter geneosnrt1.8In Oryza sativa L. selection-breeding
Application.
Background technology
Rice in China cultivated area accounts for the 20% of the total cultivated area of world crop, but amount of application of nitrogen fertilizer accounts for the world always applies
The 37% of consumption；Nineteen ninety-five China's nitrogen fertilizer production amount and usage amount reach No. 1 in the world, but nitrogenous fertilizer service efficiency is relatively low, nitrogenous fertilizer
Amount of application increase by 20 times relatively before 50 years, by this trend it is contemplated that to the year two thousand fifty, it will turn over 3 times again.The excessive meeting of nitrogen application
Lead to the ecological pollution problem such as body eutrophication [Xu Guohua, Fan Xiaorong. Oryza sativa L. nitre transporter geneosnrt1.1aWithosnrt1.1bFunctional study. Agricultural University Of Nanjing, 2011:4-6].Denitrification, water and soil are passed through in more nitrogen nutrition
Run off, naturally volatilize, microorganism using etc. approach wasted.
If the absorption efficiency of nitrogen is improved 1%, it is equivalent to save more than ten hundred million dollars of spending every year.From China
National conditions are analyzed, and the potentiality that expansion cultivated area is increased the total yield are very limited, and the only way out is raw on limited soil
The more Oryza glutinosa of output, that is, increase the yield per unit area.In traditions of the past farming, by selecting nitrogen use efficiency higher
Crop, to improve the utilization ratio of nitrogen；But compared with the breeding on molecular level, this process seems slow and poorly efficient [Zhang Hong
Journey, Dai Qigen. the genotypic difference that Nitrogen in Rice utilizes and physiological Mechanism research. Yangzhou University, 2008:10-13].Improve
Nitrogen use efficiency is it is necessary to find breach from the molecule absorption mechanism of nitrogen.Nitrate anion transporter gene family is divided into low-affinity nitre
Acid group transporter gene and high-affinity nitrate anion transporter gene two class [Zhou Shiyi. the saccharide and aminoacid high parent to Oryza sativa L. induction type
Impact with power nitrate transport system. the Central China University of Science and Technology, 2009:15-16].By nitrogen assimilation, by nitrate nitrogen and ammonium
State nitrogen absorbs and is converted into aminoacid, and the referred to as first kind of nitrogen absorbs.Increased by the transport of nitrogen is made with seed nutritional material, increase
Plus plumpness, the referred to as Equations of The Second Kind of nitrogen absorbs, that is, recycling [kant s, the bi y, steven j, et al. of nitrogen
understanding plant response to nitrogen limitation for the improvement of
crop nitrogen use efficiency . journal of experimental, 2011, 62(4): 1499-
1509].Increase nitrogen absorption and accumulation amount or N transformation amount, can increase production.Therefore, in agricultural modernization construction, by molecule
Breeding technique is improving the utilization ratio to nitrogenous fertilizer for the Oryza sativa L., it is possible to reduce nitrogenous fertilizer pollution is moreover it is possible to increase yield.
Nrt1/ptr family (nrt1/ptr family, npf) is the small molecule referring to mediate 2-3 amino acid residue
The material such as peptide and nitrate anion carries out albumen [rentsch d, schmidt s, the tegeder m. of transdermal delivery
transporters for uptake and allocation of organic nitrogen compounds in
plants. febs let, 2007, 581: 2281-2289].During nrt1/ptr family member take part in Seed Development
The accumulation of protein and micromolecule polypeptide form transhipment after protein degradation in sprouting [martre p, porter j r,
jamieson p d, et al. modeling grain nitrogen accumulation and protein
composition to understand the sink/source regulations of nitrogen
remobilization for wheat. plant physiol, 2003, 133: 1959-1967].At present with regard to npf family
The report of race member research is seldom, according to the present inventionosnrt1.8Gene is a nitrate anion fortune of Oryza sativa L. npf gene family
Defeated homologous geness.Present invention discover thatosnrt1.8Gene pairss rice tillering has extremely important effect, can be applicable to plant plant type
Improvement is so that increasing production of rice.
Content of the invention
It is an object of the invention to solution problems of the prior art, provide Oryza sativa L. npf gene family memberosnrt1.8Application in Oryza sativa L. selection-breeding for the gene.
The purpose of the present invention is achieved through the following technical solutions:
The present invention is with the npf gene family member of Oryza sativa L.osnrt1.8Gene is object, spends clone in 11 from Oryza sativa L.osnrt1.8Cdna sequence.By buildingosnrt1.8Gene overexpression vector, using Agrobacteriumeha105The heredity of mediation
Method for transformation, overexpression vector is imported in normal japonica rice variety and spends in 11, obtainosnrt1.8Gene overexpression plant, its point
Tiller number significantly improves compared with spending 11 in comparison wild type.By rnai technique constructionosnrt1.8Gene interference expression vector,
Spend during interference expression vector is imported in 11, obtainosnrt1.8The interference plant that gene expression amount declines, disturbs dividing of plant
Tiller number with spend 11 compared with significantly reduce；osnrt1.8The tiller number of mutant homozygous plants with spend 11 compared with also significantly drop
Low.These results indicate that by improvingosnrt1.8The expression of gene, can make normal rice tillering number increase, thus carrying
High spike number and rice yield.osnrt1.8Gene is in terms of illustrating Nitrogen effect plant growing and growth course and in Oryza sativa L. strain
Type improvement aspect has important using value.
Based on present invention discover thatosnrt1.8The function of gene,osnrt1.8Gene can be used in Oryza sativa L. selection-breeding.Described
Oryza sativa L. selection-breeding be improve rice tillering number, thus improving spike number and rice yield.Specifically can be by improvingosnrt1.8Gene
Expression so that rice tillering number and panicle number per hill is increased, reach the purpose improving rice yield.
osnrt1.8Gene can also be used for improving the yield of other plant, is such as made by transgenicosnrt1.8Gene is being planted
In thing, (excess) expression, to improve the branch quantity of plant, and then so that the yield of plant is improved.Described plant refers to list
Cotyledon plant or dicotyledon；As: Semen Tritici aestivi, Fructus Lycopersici esculenti, turfgrass or Herba Medicaginiss etc..
Describedosnrt1.8The aminoacid sequence of the osnrt1.8 albumen of gene code is as shown in seq id no.1；Institute
Stateosnrt1.8The cdna sequence of gene is preferably as shown in seq id no.2.
It is construed as, on the premise of not affecting osnrt1.8 protein active (i.e. not in the active center of albumen), this
Skilled person can carry out various replacements, interpolation and/or disappearance one or several to the aminoacid sequence shown in seq id no.1
Individual aminoacid obtains the aminoacid sequence with equal function.Therefore, osnrt1.8 albumen also includes ammonia shown in seq id no.1
Base acid sequence is substituted, replaces and/or increases the protein with same isoreactivity that one or several aminoacid obtain.Additionally,
It should be understood that considering the degeneracy of codon and the preferences of different plant species codon, those skilled in the art can basis
Need using the codon being suitable for particular species expression.
Advantages of the present invention and effect:
(1) present invention clonesosnrt1.8Tillering Ability in Rice is made to strengthen after gene overexpression, explanationosnrt1.8Gene pairss
Improve rice yield more apparent, therefore, improved by technique for gene engineeringosnrt1.8The expression of gene can improve plant and produce
Amount.It is not only does this facilitate and cultivates high-yield rice by under the conditions of normally applying nitrogen, the product of plant can also be carried out by molecular breeding
Plant improvement.
(2)osnrt1.8The successful clone of gene, further demonstrate that important work in nitrogen absorption process for the npf family
With having important meaning to the biological function illustrating npf family, in addition to further appreciating that plant nitrogen metabolism approach, improving
Nitrogen absorption efficiency has great impetus.
(3) although being cloned at present the gene that some improve plant products, to the molecular mechanism of plant yield-increasing still
Unclear.And present invention cloneosnrt1.8Gene can improve the yield of Oryza sativa L., to the key factor determining plant yield-increasing
There is great impetus.
Brief description
Fig. 1 be spend 11 in comparison,osnrt1.83 strains of gene overexpression plant,osnrt1.8Gene disturbs plant 3
Strain andosnrt1.8The whole strain phenotypic map of gene mutation body.
Fig. 2 be spend 11 in comparison,osnrt1.83 strains of gene overexpression plant,osnrt1.8Gene disturbs plant 3
Strain andosnrt1.8The statistics block diagram of gene mutation body tiller number, data carries out variable analyses using spss software
(anova), using duncan ' s, significance difference analysis, different group lower case (a, b, c) tables are carried out on 0.05 level
Show significant difference.
Fig. 3 be spend 11 in comparison,osnrt1.83 strains of gene overexpression plant,osnrt1.8Gene disturbs plant 3
Strain andosnrt1.8In gene mutation bodyosnrt1.8Gene relative expression's statistics of variables block diagram, data adopts spss software
Carry out variable analyses (anova), using duncan ' s, significance difference analysis, different group small letters are carried out on 0.05 level
Female (a, b, c) represents significant difference.
Specific embodiment
With reference to embodiment, the present invention will be further described in detail, but embodiments of the present invention not limited to this.
If not specializing, the conventional meanses that the technological means used by following embodiments are well known to those skilled in the art；Used
Experimental technique is conventional method, and can according to have described that recombinant technique (referring to molecular cloning, laboratory manual, second edition,
CSH Press, Cold SpringHarbor, New York) complete；Material used, reagent etc., all commercially obtain.
Embodiment 1osnrt1.8The structure of gene overexpression plant
Extract the rna spending 11 in Oryza sativa L., and its reverse transcription become cdna, using primer pair:
F1:5'-agatctGtcatggacgccggagacgccat-3'(bglIi),
R1:5'-cttaagGacgtctcacgagagcacggtct-3'(aflIi)；
Expanded by pcrosnrt1.8After the cdna of gene, pass throughbglIi andaflIt is connected into pcambia-1301 after ii enzyme action to carry
Body (pcambia-1301 carrier is purchased from cambia company), constructsosnrt1.8The overexpression vector of geneosnrt1.8-
p1301.Using Agrobacteriumeha105The genetic transforming method of mediation, overexpression vector is imported in normal rice varieties and spends 11
In.
The all transgenic plants obtaining are transplanted in the basket with soil, periodically waters, fertilising, treat that seedling grows tall about
During 10cm, plant in big Tanaka, after Seedling is grown up, extract genome dna and by pcr, transfer-gen plant is detected, with detection is drawn
Thing to for:
F2:5'-gatgttggcgacctcgtatt-3',
R2:5'-tcgttatgtttatcggcacttt-3'.
If amplifying the fragment of 517bp, illustrate that transfer-gen plant is positive plant.Positive plant individual plant sowing is simultaneously planted
Plant, until t2 generation identifies the transfer-gen plant of homozygosis, that is, obtainosnrt1.8Gene overexpression plant.osnrt1.8Gene surpasses
The tiller number of expression plant spends 11 plant far more than in comparison, and significant difference, as shown in Fig. 1,2.
Takeosnrt1.8Gene overexpression plant leaf, extracts rna and its reverse transcription is become cdna, fixed by real-time fluorescence
Amount pcr detectionosnrt1.8The expression of gene, result shows (Fig. 3) overexpression plantosnrt1.8The expression of gene with right
According in spend 11 to compare notable rising.Real time fluorescent quantitative pcr the primer to for:
F3:5'-tacgccgtcgtggaggcgttca-3',
R3:5'-tcacgagagcacggtcttgagct-3'.
Embodiment 2osnrt1.8Gene disturb plant structure andosnrt1.8The acquisition of gene mutation strain
Extract the rna spending 11 in Oryza sativa L., and its reverse transcription become cdna, using primer pair:
F4:5'-ggtaccAgcgcgtccaacgtcacgac-3'(kpnI),
R4:5'-ggatccGcgctgtcgtcgtcgaactg-3'(bamhI)；
F5:5'-actagtAgcgcgtccaacgtcacgac-3'(speI),
R5:5'-gagctcGcgctgtcgtcgtcgaactg-3'(sacI)；
Each pcr amplifiesosnrt1.8After the cdna fragment of gene, connected after above-mentioned corresponding digestion with restriction enzyme
Enter ptck303 carrier, constructosnrt1.8The interference expression vector of geneosnrt1.8-ptck303.Using Agrobacteriumeha105The genetic transforming method of mediation, interference expression vector is imported in normal japonica rice variety and spends in 11.
The all transgenic plants obtaining are transplanted in the basket with soil, periodically waters, fertilising, treat that seedling grows tall about
During 10cm, plant in big Tanaka, after Seedling is grown up, extract genome dna and by pcr, transfer-gen plant is detected, with detection is drawn
Thing to for:
F2:5'-gatgttggcgacctcgtatt-3',
R2:5'-tcgttatgtttatcggcacttt-3'.
If amplifying the fragment of 517bp, illustrate that transfer-gen plant is positive plant.Positive plant individual plant sowing is simultaneously planted
Plant, until t2 generation identifies the transfer-gen plant of homozygosis, that is, obtainosnrt1.8Gene disturbs plant.osnrt1.8Gene disturbs
The tiller number of plant spends 11 plant far fewer than in comparison, and significant difference, as shown in Fig. 1,2.
Hua Zhong Agriculture University's mutant library (http://rmd.ncpgr.cn/) is commercially availableosnrt1.8Mutation
The seed of body homozygous plants, is transplanted in the basket with soil, is periodically watered, fertilising, when seedling grows tall about 10cm, plant in
Big Tanaka, after Seedling is grown up, can get mutant plants, and the tiller number of this mutant plants spends 11 plant far fewer than in comparison,
Significant difference, as shown in Fig. 1,2.
Takeosnrt1.8Gene interference plant and mutant plants blade, extract rna and its reverse transcription are become cdna, pass through
Real time fluorescent quantitative pcr detectsosnrt1.8The expression of gene, result shows (Fig. 3) plantosnrt1.8The expression of gene
Amount significantly reduces compared with spending 11 in comparison.Real time fluorescent quantitative pcr the primer is with embodiment 1.
The above results show, by improvingosnrt1.8The expression of gene, can increase the tiller number of Oryza sativa L., and then improves
Spike number and rice yield.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not subject to above-described embodiment
Limit, other any spirit without departing from the present invention and the change made under principle, modification, replacement, combine, simplify,
All should be equivalent substitute mode, be included within protection scope of the present invention.
sequence listing
<110>Wuhan Bioengineering Institute
<120>application in Oryza sativa L. selection-breeding for the nitrate anion transporter gene osnrt1.8
<130> 1
<160> 12
<170> patentin version 3.3
<210> 1
<211> 574
<212> prt
<213> oryza sativa
<400> 1
met asp ala gly asp ala met glu ser gly gln arg ala ala leu leu
1 5 10 15
pro glu ser his gly pro lys thr glu asp asp ser leu gln val pro
20 25 30
leu leu lys asp lys lys arg gly gly ser lys ala pro ala ile val
35 40 45
leu gly phe glu cys leu glu ser thr ala phe asn gly ile ser thr
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asn leu val val tyr leu glu thr val leu his gly ser asn leu ala
65 70 75 80
ser ala ser asn val thr thr trp tyr gly thr ser tyr leu thr pro
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ile phe gly ala ile val ala asp thr phe leu gly asn tyr asn thr
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ile leu ile ser leu ala val tyr leu leu gly met met leu val thr
115 120 125
phe ser ala phe leu pro ala thr ala ala leu cys ala ala gly ala
130 135 140
thr cys gly thr gly ala ala ala ala gln thr val ala phe val gly
145 150 155 160
leu tyr leu val ala val gly ser gly gly val arg ser ser leu leu
165 170 175
pro phe gly ala glu gln phe asp asp asp ser ala ala asp arg glu
180 185 190
arg lys ala ala phe phe ser trp phe tyr leu cys val asp phe gly
195 200 205
leu ile val ser gly val leu leu val trp ile gln gln asn val ser
210 215 220
trp gly leu gly phe gly ile ala thr ala cys ile ala val ala phe
225 230 235 240
ala ala phe val leu ala thr pro met tyr lys arg arg leu pro thr
245 250 255
gly thr pro leu lys ser leu ala gln val val val ala ala phe arg
260 265 270
lys val gly met lys leu pro ala asp ala glu leu leu tyr glu val
275 280 285
ser asp lys val asp ser gln pro lys ile ala his thr ser glu phe
290 295 300
thr phe leu asp lys ala ala val val ser glu ser asp leu glu glu
305 310 315 320
arg pro glu ala ala ser ser trp lys leu cys thr val thr gln val
325 330 335
glu glu leu lys ile leu leu arg leu leu pro ile trp ala thr ser
340 345 350
ile ile val ser ala ala tyr ser gln met ser thr thr phe ile gln
355 360 365
gln gly ser ala met asp met his ile phe ser val pro val pro ala
370 375 380
ala ser leu ser ser phe gln val leu cys val leu thr trp val ile
385 390 395 400
leu tyr ser lys val ile val pro ala leu arg gly phe ser ser ser
405 410 415
gly ala ala gly glu pro ser gln leu gln arg met gly ala gly arg
420 425 430
leu leu met ala leu ala met ala val ala ala leu val glu thr lys
435 440 445
arg leu asn ala ala ala ser gly glu ala ile asn ile ala trp gln
450 455 460
met pro gln tyr phe phe leu ala gly ala glu val phe cys tyr ile
465 470 475 480
ala gln leu glu phe phe phe gly glu ala pro asp thr met lys ser
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thr cys thr ser leu ala leu leu thr ile ala leu gly ser tyr leu
500 505 510
ser ser leu ile tyr ala val val glu ala phe thr ala thr ala gly
515 520 525
gly his gly trp ile ser asp asp leu asn gln gly his leu asp tyr
530 535 540
phe phe trp met leu ala ala met cys thr leu asn phe val val tyr
545 550 555 560
ser gly phe ala lys asn tyr lys leu lys thr val leu ser
565 570
<210> 2
<211> 1725
<212> dna
<213> oryza sativa
<400> 2
atggacgccg gagacgccat ggagagcgga cagcgcgcgg cgctcctgcc cgagagtcac 60
ggaccaaaga ctgaagatga tagcctgcaa gtgccactcc tcaaggataa gaaacgcggc 120
ggcagcaagg cacctgcaat agttctaggg ttcgaatgcc tggagagcac ggcgttcaat 180
ggcatctcga cgaacctggt ggtgtacctg gagaccgtcc tccatggcag caacctggcc 240
agcgcgtcca acgtcacgac gtggtacggc acgagctacc tcaccccgat cttcggcgcc 300
atcgtcgccg acacgttcct cggcaactac aacaccatcc tcatctccct cgccgtctac 360
ctcctcggga tgatgctggt cacgttctcg gcgttcctgc cggccacggc ggcgctgtgc 420
gcggcgggcg cgacgtgcgg caccggcgcg gccgcggcgc agaccgtcgc gttcgtcggg 480
ctgtacctcg tggctgtcgg gagcggcggg gtgcggtcgt cgctgctgcc gttcggcgcc 540
gagcagttcg acgacgacag cgcggcggat agggagcgca aggcggcctt cttcagctgg 600
ttctacctct gcgtcgactt cggcctgatc gtctccggcg tgctcctcgt ctggatccag 660
cagaacgtca gctggggcct cggcttcggc atcgccaccg cgtgcatcgc ggtcgcgttc 720
gccgcgttcg tgctcgccac gcccatgtac aagcgccggt tgccgacggg cacgccgctc 780
aagagcctcg cccaggtcgt cgtcgccgcc ttcaggaagg tcggcatgaa gctccccgcc 840
gacgccgagc tcctgtacga ggtcagcgac aaggtcgact cccagcccaa gatcgcgcac 900
accagcgagt tcacgtttct tgacaaggcg gccgtcgtct cggagtcgga cctggaggag 960
aggccggagg cggcgagctc gtggaagctc tgcaccgtga cgcaggtgga ggagctcaag 1020
atccttctgc gtctgctccc catctgggcg accagcatca tcgtgtccgc ggcatactcg 1080
cagatgagca ccaccttcat ccagcaaggc agcgccatgg acatgcacat cttctcggtg 1140
ccggtgccgg cggcgtcgtt aagctccttc caggttctct gcgtcctgac atgggtgatc 1200
ctctacagca aggtgatcgt gccggcgctg aggggcttct cctcctccgg agccgccggc 1260
gagccgtcgc agctgcagcg catgggcgcc gggcgcctcc tcatggcgct cgccatggcg 1320
gtggccgcgc tcgtggagac gaagcggctc aacgccgcgg cgagcggcga ggcgatcaac 1380
atcgcgtggc agatgccgca gtacttcttc ctcgccggcg cggaggtgtt ctgctacatc 1440
gcgcagctgg agttcttctt cggcgaggcg ccggacacca tgaagagcac gtgcacgtcg 1500
ctggcgctgc tcaccatcgc gctcgggagc tacctgagct cgctcatcta cgccgtcgtg 1560
gaggcgttca cggcgacggc gggcggccac gggtggatct ccgacgacct caaccagggc 1620
cacctcgact acttcttctg gatgctggcg gccatgtgca cgctcaactt cgtcgtgtac 1680
agcgggttcg ccaagaacta caagctcaag accgtgctct cgtga 1725
<210> 3
<211> 29
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<213> artificial
<220>
<223>primer f1
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agatctgtca tggacgccgg agacgccat 29
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<213> artificial
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gatgttggcg acctcgtatt 20
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<220>
<223>primer r2
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<210> 7
<211> 22
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<213> artificial
<220>
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tacgccgtcg tggaggcgtt ca 22
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tcacgagagc acggtcttga gct 23
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<220>
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ggtaccagcg cgtccaacgt cacgac 26
<210> 10
<211> 26
<212> dna
<213> artificial
<220>
<223>primer r4
<400> 10
ggatccgcgc tgtcgtcgtc gaactg 26
<210> 11
<211> 26
<212> dna
<213> artificial
<220>
<223>primer f5
<400> 11
actagtagcg cgtccaacgt cacgac 26
<210> 12
<211> 26
<212> dna
<213> artificial
<220>
<223>primer r5
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gagctcgcgc tgtcgtcgtc gaactg 26
Claims (7)
1.osnrt1.8Application in Oryza sativa L. selection-breeding for the gene it is characterised in that: described Oryza sativa L. selection-breeding is to improve rice tillering
Number.
2. according to claim 1 application it is characterised in that: by improveosnrt1.8The expression of gene makes rice tillering
Number increases.
3.osnrt1.8Application in improving plant products for the gene.
4. according to claim 3 application it is characterised in that: described plant refers to monocotyledon or dicotyledon.
5. the application according to claim 3 or 4 it is characterised in that: described plant include Semen Tritici aestivi, Fructus Lycopersici esculenti, turfgrass or
Herba Medicaginiss.
6. the application according to any one of claim 1-3 it is characterised in that: describedosnrt1.8Gene code
The aminoacid sequence of osnrt1.8 albumen is as shown in seq id no.1；Or osnrt1.8 albumen is amino shown in seq id no.1
Acid sequence is substituted, replaces and/or increases the protein with same isoreactivity that one or several aminoacid obtain.
7. according to claim 6 application it is characterised in that: describedosnrt1.8The cdna sequence of gene such as seq id
Shown in no.2.
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Cited By (8)
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| CN107012153A (en) * | 2017-03-30 | 2017-08-04 | 武汉生物工程学院 | Applications of the nitrogen nutrition transporter gene OsNPF8.1 in rice tillering number is improved |
| CN108034672A (en) * | 2017-12-19 | 2018-05-15 | 武汉生物工程学院 | Applications of the nitrate anion transporter gene OsNRT1.9b in rice selection and breeding |
| CN108034661A (en) * | 2017-12-19 | 2018-05-15 | 武汉生物工程学院 | Application of the OsNPF8.8b genes in rice yield and nutritional quality is improved |
| CN108070601A (en) * | 2017-12-19 | 2018-05-25 | 武汉生物工程学院 | Application of the OsNPF8.6b genes in rice yield is improved |
| CN108118062A (en) * | 2017-12-19 | 2018-06-05 | 武汉生物工程学院 | Applications of the nitrate anion transporter gene OsNRT1.9a in rice selection and breeding |
| CN108409844A (en) * | 2018-05-16 | 2018-08-17 | 中国科学院遗传与发育生物学研究所 | Applications of the protein TaNRT2.5 in regulating and controlling plant products |
| CN110982828A (en) * | 2020-01-02 | 2020-04-10 | 南京农业大学 | Nitrate transport protein gene specifically induced by rice arbuscular mycorrhiza and application thereof |
| CN111748560A (en) * | 2017-12-28 | 2020-10-09 | 南京农业大学 | Application of rice OsNRT2.1 gene in improving manganese content in rice grains |
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| CN107012153A (en) * | 2017-03-30 | 2017-08-04 | 武汉生物工程学院 | Applications of the nitrogen nutrition transporter gene OsNPF8.1 in rice tillering number is improved |
| CN107012153B (en) * | 2017-03-30 | 2020-05-29 | 武汉生物工程学院 | Application of nitrogen nutrition transport gene OsNPF8.1 in improving tillering number of rice |
| CN108034661B (en) * | 2017-12-19 | 2020-05-29 | 武汉生物工程学院 | Application of OsNPF8.8b gene in improving rice yield and nutrition quality |
| CN108070601A (en) * | 2017-12-19 | 2018-05-25 | 武汉生物工程学院 | Application of the OsNPF8.6b genes in rice yield is improved |
| CN108118062A (en) * | 2017-12-19 | 2018-06-05 | 武汉生物工程学院 | Applications of the nitrate anion transporter gene OsNRT1.9a in rice selection and breeding |
| CN108034672B (en) * | 2017-12-19 | 2020-05-29 | 武汉生物工程学院 | Application of nitrate transport gene OsNRT1.9b in rice breeding |
| CN108118062B (en) * | 2017-12-19 | 2020-05-29 | 武汉生物工程学院 | Application of nitrate transport gene OsNRT1.9a in rice breeding |
| CN108034661A (en) * | 2017-12-19 | 2018-05-15 | 武汉生物工程学院 | Application of the OsNPF8.8b genes in rice yield and nutritional quality is improved |
| CN108034672A (en) * | 2017-12-19 | 2018-05-15 | 武汉生物工程学院 | Applications of the nitrate anion transporter gene OsNRT1.9b in rice selection and breeding |
| CN108070601B (en) * | 2017-12-19 | 2020-07-07 | 武汉生物工程学院 | Application of OsNPF8.6b gene in increasing rice yield |
| CN111748560A (en) * | 2017-12-28 | 2020-10-09 | 南京农业大学 | Application of rice OsNRT2.1 gene in improving manganese content in rice grains |
| CN111748560B (en) * | 2017-12-28 | 2022-03-29 | 南京农业大学 | Application of rice OsNRT2.1 gene in improving manganese content in rice grains |
| CN108409844A (en) * | 2018-05-16 | 2018-08-17 | 中国科学院遗传与发育生物学研究所 | Applications of the protein TaNRT2.5 in regulating and controlling plant products |
| CN110982828A (en) * | 2020-01-02 | 2020-04-10 | 南京农业大学 | Nitrate transport protein gene specifically induced by rice arbuscular mycorrhiza and application thereof |
| CN110982828B (en) * | 2020-01-02 | 2022-08-30 | 南京农业大学 | Nitrate transport protein gene specifically induced by rice arbuscular mycorrhiza and application thereof |
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