CN106290816B - Chemiluminescence immune assay device - Google Patents
Chemiluminescence immune assay device Download PDFInfo
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- CN106290816B CN106290816B CN201610534057.9A CN201610534057A CN106290816B CN 106290816 B CN106290816 B CN 106290816B CN 201610534057 A CN201610534057 A CN 201610534057A CN 106290816 B CN106290816 B CN 106290816B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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Abstract
The present invention relates to a kind of chemiluminescence immune assay devices, belong to the field of medical instrument technology.The chemiluminescence immune assay device of the present invention, including sample response analysis system and single electron counter, sample response analysis system is equipped with postposition fixed plate, sliding axle is equipped on front side of postposition fixed plate, sample luminescence-producing reaction drip irrigation emitter is equipped on front side of sliding axle, grumeleuse reaction liquid drip irrigation emitter is equipped on the right side of sample luminescence-producing reaction drip irrigation emitter, detection operation platform is equipped on the downside of postposition fixed plate, it detects operation platform and is equipped with serum placement detection case, serum places detection case and is internally provided with rack for test tube fixation socket, and rack for test tube, which is fixed, is equipped with rack for test tube on front side of socket.The present invention is multiple functional, easy to use, and when carrying out chemiluminescence immune assay, operation flexibly, can analyze sero-immunity ability comprehensively, network analysis data, greatly alleviate the work load of medical staff.
Description
Technical field
The invention belongs to the field of medical instrument technology, and in particular to a kind of chemiluminescence immune assay device.
Background technology
Chemiluminescence immune assay has extremely important effect during disease control, passes through chemiluminescence immunoassay point
Analysis can be apparent from the immune state of patient's body, where understanding in time and pinpointing the problems, present immunoassay dress
It puts majority to analyze by semi-automatic realize, take great energy arduously, and inevitably cause analysis result mistake there are human error, and then
Make treatment generate deviation, if things go on like this, the state of an illness of patient not but not improve, patient health can be endangered instead, also to medicine
Inhibition is played in development.
The content of the invention
Multiple functional the object of the present invention is to provide one kind during chemiluminescence immune assay is carried out, safety is high
The chemiluminescence immune assay device of effect.
The technical scheme is that：Chemiluminescence immune assay device, including sample response analysis system and single electron
Counter, sample response analysis system are equipped with postposition fixed plate, sliding axle are equipped on front side of postposition fixed plate, is set on front side of sliding axle
There is sample luminescence-producing reaction drip irrigation emitter, grumeleuse reaction liquid drip irrigation emitter, postposition fixed plate are equipped on the right side of sample luminescence-producing reaction drip irrigation emitter
Downside is equipped with detection operation platform, and detection operation platform is equipped with serum and places detection case, and serum is placed inside detection case
Socket is fixed equipped with rack for test tube, rack for test tube is fixed and rack for test tube is equipped on front side of socket, and rack for test tube placing groove, blood are equipped on the downside of rack for test tube
Clear place is equipped with test tube coding on front side of detection case, seal glass is equipped on front side of test tube coding, rotation axis is equipped on the downside of seal glass,
Serum, which is placed, is equipped with grumeleuse reaction chamber on the right side of detection case, grumeleuse reaction chamber is internally provided with test tube and assigns lattice, and test tube is assigned inside lattice
Equipped with plastics reaction tube, test tube, which is assigned to be equipped on front side of lattice, ranks coding, and printer box, printer box are equipped on the right side of grumeleuse reaction chamber
Upside is equipped with printing paper socket, and printing paper delivery is equipped on front side of printer box, is equipped on the downside of printing paper delivery and sets button, set
Instrument switch is equipped on the right side of button, low temperature controlling machine box are equipped on the downside of detection operation platform, are equipped on the right side of low temperature controlling machine box
Power lead exports, and power lead outlet is internally provided with power lead, and attaching plug, low temperature control machine are equipped on the downside of power lead
Bottom support feet is equipped on the downside of case；
Single electron counter is equipped with photomultiplier, and being equipped with photoelectron on the left of photomultiplier excites cathode, photoelectron
It excites to be equipped on the right side of cathode and doubles pole, double ultra-Right side equipped with anode, anode is equipped with signal output device, signal output device right side
Equipped with output lead, coil is equipped on the downside of output lead, connecting wire is equipped on the downside of coil, is equipped on the left of connecting wire and puts at a high speed
Big device, high speed amplifier left side are equipped with comparator connecting wire, high-speed comparator, high ratio are equipped on the left of comparator connecting wire
Compared with output of pulse signal line is equipped on the left of device, high-speed frequency divider is equipped on the left of output of pulse signal line, is set on the left of high-speed frequency divider
There is digit pulse output line, high-voltage conducting wires are equipped on the downside of photomultiplier, high-voltage conducting wires are equipped with resistance；
Sample luminescence-producing reaction drip irrigation emitter, which is equipped with, slides dropper case, slides dropper case and is internally provided with back-and-forth motion axis, front and rear
Liquid-drop machine is equipped on front side of shifting axle, is equipped on the upside of liquid-drop machine and is tightly connected plug, side beyond the Great Wall is tightly connected and is equipped with catheter, catheter
Downside is equipped with connecting pole, and rubber stopper is equipped on the downside of connecting pole, and drug is equipped on the downside of rubber stopper and exports tank, drug output tank is equipped with
Scale, drug output tank are internally provided with draft tube, are equipped with test solution storage box on the right side of drug output tank, test solution storage box is internally provided with
Test solution pipe is concentrated, wide opening tube head is equipped on the downside of liquid-drop machine, thin mouth tube head is equipped on the downside of wide opening tube head, slides and be equipped on front side of dropper case
Embedded groove, slides and collection spool is equipped on rear side of dropper case, and collection spool is internally provided with computer controller connector；
Low temperature controlling machine box are equipped with sealed cabinet, and sealed cabinet is internally provided with evaporator, and evaporator is internally provided with evaporation
It manages, steam nozzle is equipped on the right side of evaporator, steam delivery line is equipped on the right side of steam nozzle, steam delivery line downside is equipped with condensation
Device, condenser are internally provided with communicating pipe, and ingress pipe is equipped on the left of condenser, are equipped with compressor on the left of ingress pipe, inside compressor
Equipped with compression turbine, compression turbine interior is equipped with impeller, and circulation pipe is equipped on the left of compressor.
Preferably, the postposition fixed plate outer sheath is equipped with preposition slide-bar.
Preferably, the detection operation platform is equipped with data display equipment.
Preferably, the serum, which places detection case, is internally provided with refrigeration lamp.
Preferably, the serum, which is placed, is equipped with dust excluding plate on the downside of detection case.
Preferably, glass plate is equipped on front side of the grumeleuse reaction chamber.
Preferably, the liquid-drop machine is equipped with flow control device.
Preferably, device for drying and filtering is equipped on the upside of the condenser.
The beneficial effects of the invention are as follows：The present invention is multiple functional, easy to use, when carrying out chemiluminescence immune assay,
Operation is flexible, can analyze sero-immunity ability comprehensively, network analysis data, greatly alleviates the work load of medical staff.
Description of the drawings
Attached drawing 1 is overall structure diagram of the invention.
Attached drawing 2 is single electron counter structure schematic diagram of the invention.
Attached drawing 3 is sample luminescence-producing reaction drip irrigation emitter structure diagram of the invention.
Attached drawing 4 is low temperature controlling machine box structure diagram of the invention.
In figure：
1：Sample response analysis system, 2：Single electron counter, 3：Postposition fixed plate, 4：Sliding axle, 5：Sample shines instead
Answer drip irrigation emitter, 6：Grumeleuse reaction liquid drip irrigation emitter, 7：Detection operation platform, 8：Serum placement detection case, 9：Rack for test tube is fixed
Socket, 10：Rack for test tube, 11：Rack for test tube placing groove, 12：Test tube coding, 13：Seal glass, 14：Rotation axis, 15：Grumeleuse reacts
Case, 16：Test tube assigns lattice, and 17：Plastics reaction tube, 18：Rank coding, 19：Printer box, 20：Printing paper socket, 21：Printing
Paper delivery, 22：Setting button, 23：Instrument switch, 24：Low temperature controlling machine box, 25：Power lead exports, and 26：Power lead,
27：Attaching plug, 28：Bottom support feet, 29：Photomultiplier, 30：Photoelectron excitation cathode, 31：Double pole, 32：Anode,
33：Signal output device, 34：Output lead, 35：Coil, 36：Connecting wire, 37：High speed amplifier, 38：Comparator connection is led
Line, 39：High-speed comparator, 40：Output of pulse signal line, 41：High-speed frequency divider, 42：Digit pulse output line, 43：High pressure is led
Line, 44：Resistance, 45：Slip dropper case, 46：Back-and-forth motion axis, 47：Liquid-drop machine, 48：It is tightly connected and fills in, 49：Catheter, 50：
Connecting pole, 51：Rubber stopper, 52：Drug output tank, 53：Scale, 54：Draft tube, 55：Test solution storage box, 56：Test solution pipe is concentrated,
57：Wide opening tube head, 58：Thin mouth tube head, 59：Embedded groove, 60：Collection spool, 61：Computer controller connector, 62：Sealed cabinet,
63：Evaporator, 64：Evaporation tube, 65：Steam nozzle, 66：Steam delivery line, 67：Condenser, 68：Communicating pipe, 69：Ingress pipe,
70：Compressor, 71：Compression turbine, 72：Impeller, 73：Circulation pipe, 74：Preposition slide-bar, 75：Data display equipment, 76：Refrigeration lamp,
77：Dust excluding plate, 78：Glass plate, 79：Flow control device, 80：Device for drying and filtering.
Specific embodiment
With reference to the accompanying drawings, the chemiluminescence immune assay device of the present invention is described in detail.
As shown in Figure 1, the chemiluminescence immune assay device of the present invention, including sample response analysis system 1 and single electron
Counter 2, sample response analysis system 1 are equipped with postposition fixed plate 3, and 3 front side of postposition fixed plate is equipped with sliding axle 4, sliding axle 4
Front side is equipped with sample luminescence-producing reaction drip irrigation emitter 5, and 5 right side of sample luminescence-producing reaction drip irrigation emitter is equipped with grumeleuse reaction liquid drip irrigation emitter 6, after
It puts 3 downside of fixed plate and is equipped with detection operation platform 7, detection operation platform 7 is equipped with serum and places detection case 8, and serum is put
It puts detection case 8 and is internally provided with rack for test tube fixation socket 9, rack for test tube fixes 9 front side of socket and is equipped with rack for test tube 10,10 downside of rack for test tube
Equipped with rack for test tube placing groove 11, serum places 8 front side of detection case and is equipped with test tube coding 12, and 12 front side of test tube coding is equipped with sealing glass
Glass 13,13 downside of seal glass are equipped with rotation axis 14, and serum places 8 right side of detection case and is equipped with grumeleuse reaction chamber 15, grumeleuse reaction
Case 15 is internally provided with test tube and assigns lattice 16, and test tube assigns lattice 16 to be internally provided with plastics reaction tube 17, and test tube assigns 16 front side of lattice to set
Have and rank coding 18,15 right side of grumeleuse reaction chamber is equipped with printer box 19, and 19 upside of printer box is equipped with printing paper socket 20, beats
It prints 19 front side of cabinet and is equipped with printing paper delivery 21,21 downside of printing paper delivery, which is equipped with, sets button 22, and 22 right side of button is set to set
There is instrument switch 23,7 downside of detection operation platform is equipped with low temperature controlling machine box 24, and 24 right side of low temperature controlling machine box is equipped with power supply
Wire outlet 25, power lead outlet 25 are internally provided with power lead 26, and 26 downside of power lead is equipped with attaching plug 27, low temperature
24 downside of controlling machine box is equipped with bottom support feet 28；
As shown in Fig. 2, single electron counter 2 is equipped with photomultiplier 29,29 left side of photomultiplier is equipped with photoelectron
Cathode 30 is excited, 30 right side of photoelectron excitation cathode, which is equipped with, doubles pole 31, doubles 31 right side of pole and is equipped with anode 32, is set on anode 32
There is signal output device 33,33 right side of signal output device is equipped with output lead 34, and 34 downside of output lead is equipped with coil 35, coil 35
Downside is equipped with connecting wire 36, and 36 left side of connecting wire is equipped with high speed amplifier 37, and 37 left side of high speed amplifier is equipped with comparator
Connecting wire 38,38 left side of comparator connecting wire are equipped with high-speed comparator 39, and 39 left side of high-speed comparator is equipped with pulse signal
Output line 40,40 left side of output of pulse signal line are equipped with high-speed frequency divider 41, and it is defeated that 41 left side of high-speed frequency divider is equipped with digit pulse
Outlet 42,29 downside of photomultiplier are equipped with high-voltage conducting wires 43, and high-voltage conducting wires 43 are equipped with resistance 44；
As shown in figure 3, sample luminescence-producing reaction drip irrigation emitter 5, which is equipped with, slides dropper case 45, slide dropper case 45 and be internally provided with
Axis 46 is moved back and forth, 46 front side of axis is moved back and forth and is equipped with liquid-drop machine 47,47 upside of liquid-drop machine, which is equipped with, is tightly connected plug 48, and sealing connects
It connects 48 upside of plug and is equipped with catheter 49,49 downside of catheter is equipped with connecting pole 50, and 50 downside of connecting pole is equipped with rubber stopper 51, rubber
51 downside of plug is equipped with drug and exports tank 52, and drug output tank 52 is equipped with scale 53, and drug output tank 52 is internally provided with draft tube
54,52 right side of drug output tank is equipped with test solution storage box 55, and test solution storage box 55 is internally provided with concentration test solution pipe 56, liquid-drop machine 47
Downside is equipped with wide opening tube head 57, and 57 downside of wide opening tube head is equipped with thin mouth tube head 58, slides 45 front side of dropper case and is equipped with embedded groove 59,
It slides 45 rear side of dropper case and is equipped with collection spool 60, collection spool 60 is internally provided with computer controller connector 61；
As shown in figure 4, low temperature controlling machine box 24 are equipped with sealed cabinet 62, sealed cabinet 62 is internally provided with evaporator 63,
Evaporator 63 is internally provided with evaporation tube 64, and 63 right side of evaporator is equipped with steam nozzle 65, and 65 right side of steam nozzle is led equipped with steam
Outlet pipe 66,66 downside of steam delivery line are equipped with condenser 67, and condenser 67 is internally provided with communicating pipe 68, and 67 left side of condenser is equipped with
Ingress pipe 69,69 left side of ingress pipe are equipped with compressor 70, and compressor 70 is internally provided with compression turbine 71, compresses and set inside turbine 71
There is impeller 72,70 left side of compressor is equipped with circulation pipe 73.
When carrying out chemiluminescence immune assay, the attaching plug 27 in sample response analysis system 1 is connected with power supply,
Low temperature controlling machine box 24 is kept to open instrument switch 23 in operating status, serum is kept to place detection case 8 and grumeleuse reaction chamber
15 inside will add in sample serum, be placed in rack for test tube 10, be fixed on rack for test tube placing groove 11 in low-temperature condition in test tube
It is interior, remember the serum sample corresponding to test tube coding 12, the chemical reagent for needing trickle irrigation is inputted in computer, computer passes through computer
Controller connector 61 controls the trickle irrigation speed of liquid-drop machine 47 and the chemical reagent of trickle irrigation, and luminescence-producing reaction occurs in serum,
It is counted by single electron counter 2, photoelectron is continuously increased in 29 self-energy of photomultiplier and number, and reaching can count
The level of calculation, immunocompetence is drawn by the analysis of single electron counter 2, and analysis result is printed by printer box 19.
3 outer sheath of postposition fixed plate is equipped with preposition slide-bar 74.It in this way, can be with fixed sample luminescence-producing reaction trickle irrigation
Device 5, while it can be aided in horizontally slip.
The detection operation platform 7 is equipped with data display equipment 75.In this way, it can show seroreaction in test tube
Situation, to determine the reaction time.
The serum places detection case 8 and is internally provided with refrigeration lamp 76.In this way, serum can be kept to place detection case 8
Low-temperature condition.
The serum places 8 downside of detection case and is equipped with dust excluding plate 77.In this way, it can be shut after use dust-proof
Plate 77 completely cuts off external dust.
15 front side of grumeleuse reaction chamber is equipped with glass plate 78.In this way, it can prevent liquid from spilling.
The liquid-drop machine 47 is equipped with flow control device 79.In this way, chemical reagent discharge can be controlled.
67 upside of condenser is equipped with device for drying and filtering 80.In this way, it can be made not to be deposited on cold with impurity screening
In condenser 67.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the technical principles of the invention, several improvement and replacement can also be made, these improve and replace
Also it should be regarded as protection scope of the present invention.
Claims (8)
1. chemiluminescence immune assay device, including sample response analysis system (1) and single electron counter (2), feature exists
In：
Sample response analysis system (1) is equipped with postposition fixed plate (3), and sliding axle (4) is equipped on front side of postposition fixed plate (3), sliding
Sample luminescence-producing reaction drip irrigation emitter (5) is equipped on front side of moving axis (4), grumeleuse reaction solution is equipped on the right side of sample luminescence-producing reaction drip irrigation emitter (5)
Body drip irrigation emitter (6), postposition fixed plate (3) downside are equipped with detection operation platform (7), and detection operation platform (7) is equipped with blood
Clear to place detection case (8), serum places detection case (8) and is internally provided with rack for test tube fixation socket (9), and rack for test tube fixes socket (9)
Front side is equipped with rack for test tube (10), and rack for test tube placing groove (11) is equipped on the downside of rack for test tube (10), and serum is placed to be set on front side of detection case (8)
There is test tube coding (12), seal glass (13) is equipped on front side of test tube coding (12), rotation axis is equipped on the downside of seal glass (13)
(14), serum, which is placed, is equipped with grumeleuse reaction chamber (15) on the right side of detection case (8), grumeleuse reaction chamber (15) is internally provided with test tube and assigns lattice
(16), test tube assigns lattice (16) to be internally provided with plastics reaction tube (17), and test tube, which is assigned to be equipped on front side of lattice (16), ranks coding (18),
Printer box (19) is equipped on the right side of grumeleuse reaction chamber (15), printing paper socket (20), printer are equipped on the upside of printer box (19)
Printing paper delivery (21) is equipped on front side of case (19), is equipped on the downside of printing paper delivery (21) and button (22) is set, button (22) is set
Right side is equipped with instrument switch (23), and low temperature controlling machine box (24), low temperature controlling machine box are equipped on the downside of detection operation platform (7)
(24) right side is equipped with power lead and exports (25), and power lead outlet (25) is internally provided with power lead (26), power lead
(26) downside is equipped with attaching plug (27), and bottom support feet (28) is equipped on the downside of low temperature controlling machine box (24)；
Single electron counter (2) is equipped with photomultiplier (29), and being equipped with photoelectron on the left of photomultiplier (29) excites cathode
(30), it is equipped on the right side of photoelectron excitation cathode (30) and doubles pole (31), is doubled and anode (32), anode (32) are equipped on the right side of pole (31)
Signal output device (33) is equipped with, output lead (34) is equipped on the right side of signal output device (33), is equipped on the downside of output lead (34)
Coil (35), coil (35) downside are equipped with connecting wire (36), high speed amplifier (37) are equipped on the left of connecting wire (36), at a high speed
Comparator connecting wire (38) is equipped on the left of amplifier (37), high-speed comparator is equipped on the left of comparator connecting wire (38)
(39), output of pulse signal line (40) is equipped on the left of high-speed comparator (39), is equipped at a high speed on the left of output of pulse signal line (40)
Frequency divider (41), high-speed frequency divider (41) left side are equipped with digit pulse output line (42), height are equipped on the downside of photomultiplier (29)
Conducting wire (43) is pressed, high-voltage conducting wires (43) are equipped with resistance (44)；
Sample luminescence-producing reaction drip irrigation emitter (5), which is equipped with, slides dropper case (45), slides dropper case (45) and is internally provided with back-and-forth motion
Axis (46) moves back and forth and liquid-drop machine (47) is equipped on front side of axis (46), is equipped on the upside of liquid-drop machine (47) and is tightly connected plug (48), sealing
Catheter (49) is equipped on the upside of connection plug (48), connecting pole (50) is equipped on the downside of catheter (49), is equipped on the downside of connecting pole (50)
Rubber stopper (51), rubber stopper (51) downside are equipped with drug output tank (52), and drug output tank (52) is equipped with scale (53), drug
Output tank (52) is internally provided with draft tube (54), and test solution storage box (55), test solution storage box are equipped on the right side of drug output tank (52)
(55) concentration test solution pipe (56) is internally provided with, wide opening tube head (57) is equipped on the downside of liquid-drop machine (47), is set on the downside of wide opening tube head (57)
There is thin mouth tube head (58), slide and embedded groove (59) is equipped on front side of dropper case (45), slide and collection spool is equipped on rear side of dropper case (45)
(60), collect spool (60) and be internally provided with computer controller connector (61)；
Low temperature controlling machine box (24) are equipped with sealed cabinet (62), and sealed cabinet (62) is internally provided with evaporator (63), evaporator
(63) evaporation tube (64) is internally provided with, steam nozzle (65) is equipped on the right side of evaporator (63), is equipped with and steams on the right side of steam nozzle (65)
Vapour delivery line (66), steam delivery line (66) downside are equipped with condenser (67), and condenser (67) is internally provided with communicating pipe (68), cold
Ingress pipe (69) is equipped on the left of condenser (67), is equipped with compressor (70) on the left of ingress pipe (69), compressor (70) is internally provided with pressure
Contracting turbine (71), compression turbine (71) are internally provided with impeller (72), circulation pipe (73) are equipped on the left of compressor (70).
2. chemiluminescence immune assay device according to claim 1, it is characterised in that：The postposition fixed plate (3) is outside
Side is arranged with preposition slide-bar (74).
3. chemiluminescence immune assay device according to claim 1, it is characterised in that：The detection operation platform
(7) it is equipped with data display equipment (75).
4. chemiluminescence immune assay device according to claim 1, it is characterised in that：The serum places detection case
(8) it is internally provided with refrigeration lamp (76).
5. chemiluminescence immune assay device according to claim 1, it is characterised in that：The serum places detection case
(8) downside is equipped with dust excluding plate (77).
6. chemiluminescence immune assay device according to claim 1, it is characterised in that：Before the grumeleuse reaction chamber (15)
Side is equipped with glass plate (78).
7. chemiluminescence immune assay device according to claim 1, it is characterised in that：The liquid-drop machine (47) is equipped with
Flow control device (79).
8. according to claim 1-7 any one of them chemiluminescence immune assay devices, it is characterised in that：The condenser
(67) upside is equipped with device for drying and filtering (80).
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| CN105277726A (en) * | 2014-07-25 | 2016-01-27 | 广州瑞博奥生物科技有限公司 | Protein chip fully-automated high-throughput analysis method and protein chip fully-automated high-throughput analysis apparatus |
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