CN106290537A - The method of L-type Tryptophan concentration in detection solution - Google Patents
The method of L-type Tryptophan concentration in detection solution Download PDFInfo
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/308—Electrodes, e.g. test electrodes; Half-cells at least partially made of carbon
Abstract
The invention discloses and a kind of detect the method for L-type Tryptophan concentration in solution, three-electrode system is used by differential pulse voltammetry, the L-type tryptophan in solution to be measured to be detected, linear equation according to L-type tryptophan obtains the concentration of L-type tryptophan in solution to be measured, and the working electrode in three-electrode system is the modified glassy carbon electrode that glass-carbon electrode obtains after amination graphene quantum dot and beta cyclodextrin are modified.The method of the present invention can quickly detect L-type Tryptophan concentration, and highly sensitive, accuracy is high.The present invention builds relevant sensing interface as three-electrode system sensor for detecting, use current potential to carry out tryptophan configuration and carry out qualitative, use out peak point current to carry out quantitatively, improve the detection susceptiveness of L-type tryptophan and accuracy, minimum can detect 8.5 × 10‑7The L-type tryptophan of mol/L.The method of the present invention greatly reduces testing cost, simple to operation.
Description
Technical field
The present invention relates to heterogeneous amino acid detection techniques field.It is more particularly related to one is based on amino
The electrochemical sensing that functionalized graphene quantum dot and beta cyclodextrin are modified utilizes L-type tryptophan in differential pulse voltammetry detection solution
The method of concentration.
Technical background
Tryptophan is the precursor substance that auxin biosynthesis is important in plant, and its structure is similar to heteroauxing,
Higher plant generally exists.Tryptophan synthetic auxin can be passed through.This product is important nutrient.May participate in animal body
The renewal of plasma proteins, and riboflavin can be promoted to play a role, additionally aid the synthesis of nicotinic acid and haemachrome, can dramatically increase
Pregnant animal tire son's internal antibody, has promotion Lactation to lactational milk cattle and sow.When poultry lack tryptophan, raw
Long stagnation, weight loss, Fat Accumulation reduces, sire atrophy of testis.In the control agent being pharmaceutically used as pellagra.
Owing to the outer rim of beta cyclodextrin is hydrophilic, inner chamber is hydrophobic, thus it can provide a hydrophobic combination as enzyme
Position, as the various suitable object of main body envelope, such as organic molecule, inorganic ions and gas molecule etc..Its inner chamber is hydrophobic
And the characteristic of external hydrophilic make its can according to Van der Waals force, hydrophobic interaction power, the intermolecular matching effect of Subjective and Objective etc. with
Many organic and inorganic molecule forms clathrate and molecular assembled system, and the research becoming chemistry interested with chemical research person is right
As.The molecular recognition that this selective tetra-inclusion complex is the most usually said, its result is to form Subjective and Objective inclusion complex.Beta cyclodextrin
It is the preferable host molecule being similar to enzyme found so far, and itself just has the characteristic of catalator.Therefore, catalysis,
In the fields such as separation, food and medicine, beta cyclodextrin receives to be paid attention to greatly and extensively applies.
The method that the aminoacid of existing detection various configuration has cyclic voltammetric and AC impedance, these methods are to it
Middle a kind of index as according to carry out simultaneously qualitative and quantitative analysis, such as cyclic voltammetric simply using the size of current signal as
Distinguishing the aminoacid of various configuration, AC impedance is using resistance size as differentiation, and its accuracy is relatively low, and sensitivity is the lowest, inspection
Go out limit for height.
Summary of the invention
It is an object of the invention to solve at least the above or defect, and the advantage that at least will be described later is provided.
It is a still further object of the present invention to provide and a kind of detect the method for L-type Tryptophan concentration in solution, it can be quick
And the content of L-type tryptophan in detection by quantitative solution, establish a kind of simple, quickly and sensitivity and the high L-type color of accuracy
Propylhomoserin detection method.
It is a still further object of the present invention to provide the preparation method of working electrode, use through amination graphene quantum dot
With beta cyclodextrin modified glassy carbon electrode as working electrode, the method for preparation work electrode is simple.
In order to realize according to object of the present invention and further advantage, it is provided that L-type tryptophan in a kind of detection solution
The method of concentration, wherein, including: build the linear equation of L-type tryptophan in advance, use by working electrode, reference electrode and auxiliary
The three-electrode system helping electrode to constitute carries out detection by differential pulse voltammetry to the L-type tryptophan in solution to be measured and obtains phase
Answering parameter, the linear equation that relevant parameter substitutes into described L-type tryptophan obtains the concentration of L-type tryptophan in solution to be measured；
Wherein, described working electrode is that glass-carbon electrode obtains after amination graphene quantum dot and beta cyclodextrin are modified
Modified glassy carbon electrode.
Preferably, in described detection solution in the method for L-type Tryptophan concentration, the preparation of described modified glassy carbon electrode
Method includes:
Reference electrode, auxiliary electrode and glass-carbon electrode are immersed containing 1.5～2.5mg/mL amination graphene quantum dots
With in the mixed solution of 1.5～2.5mg/mL beta cyclodextrins, take out after utilizing cyclic voltammetric method to scan described in being drying to obtain
Modified glassy carbon electrode；
Wherein, sweep parameter is set to: initial potential 0V, maximum potential 1V, minimum point position 0V, final current potential 0V, scanning
Speed 0.1V/s, scanning times 100 times, sensitivity 10-4A/V, waiting time are 2s.
Preferably, in described detection solution in the method for L-type Tryptophan concentration, the line of L-type tryptophan is built in advance
Property equation comprises the following steps:
Step one, compound concentration are the standard solution of at least 4 parts of L-type tryptophans of 7.0～30.0 μm ol/L；
Step 2, use described three-electrode system, utilize differential pulse voltammetry that every part of standard solution is detected,
To the differential pulse voltammetry curve of every part of standard solution, record the differential pulse voltammetry of every part of standard solution the most respectively
The peak value of current intensity in curve；
Step 3, using the peak value of current intensity corresponding to the differential pulse voltammetry curve of every part of standard solution of gained as
Vertical coordinate, draws standard curve with the concentration of every part of standard solution for abscissa and is calculated the linear equation of L-type tryptophan.
Preferably, in described detection solution in the method for L-type Tryptophan concentration, described three-electrode system is used, profit
With differential pulse voltammetry, the solution to be measured containing unknown concentration L-type tryptophan is detected, obtain the difference of solution to be measured
Pulse Voltammetry curve, finds out the peak parameters of current intensity corresponding to the differential pulse voltammetry curve of solution to be measured, and by described
The linear equation of the L-type tryptophan described in peak value substitution, i.e. obtains the concentration of L-type tryptophan in solution to be measured after calculating.
Preferably, in described detection solution in the method for L-type Tryptophan concentration, L-type tryptophan in described step one
Standard solution collocation method be: being 6.5～7.5 to pH, concentration is 10mmol/L phosphate buffer solution and concentration is
The L-type tryptophan solution mixing of 0.01mol/L, preparing L-type Tryptophan concentration the most respectively is 7.0 × 10-6mol/L、10.0×
10-6mol/L、15.0×10-5mol/L、3.0×10-54 standard solution of mol/L.
Preferably, in described detection solution in the method for L-type Tryptophan concentration, described step 2 specifically includes following
Step:
1) described three-electrode system is respectively implanted in described 4 standard solution, under 20～30 DEG C of room temperatures, stirs 1min,
Static 1min；
2) scanning differential pulse collection of illustrative plates, arranging scanning initial potential is-0.1V, and termination current potential is 0.6V, and current potential increment is
0.004V, Sample Width 0.0167s, amplitude is 0.05V, pulse width 0.05V, sensitivity 10-4A/V, the waiting time is 2s；
3) measure and record the peak value of current intensity of described 4 standard solution respectively, set up described 4 standard solution
Differential pulse voltammetry curve.
Preferably, in described detection solution in the method for L-type Tryptophan concentration, described amination graphene quantum dot
Obtain after being concentrated by the amination rotated evaporimeter of graphene quantum dot that concentration is 1mg/mL.
Preferably, in described detection solution in the method for L-type Tryptophan concentration, before described modified glassy carbon electrode uses
Through pretreatment, pretreatment comprises the following steps:
Modified glassy carbon electrode is placed on the polishing cloth of the polishing powder containing granularity 0.3 μm and is polished to minute surface, subsequently will
Glass-carbon electrode is sequentially placed into methanol, concentration is the H of 0.5mol/L2SO4Difference ultrasonic 25～35s in solution and ultra-pure water, and often
Described modified glassy carbon electrode is obtained with ultrapure water 0.5～1.5min after secondary ultrasonic end.
Preferably, in described detection solution in the method for L-type Tryptophan concentration, reference electrode is Ag/AgCl electrode,
Auxiliary electrode is platinum electrode.
The method have the advantages that
First, the method for the invention uses amination graphene quantum dot and beta cyclodextrin modified glassy carbon electrode to prepare work
Make electrode, build relevant sensing interface and be used for detecting as three-electrode system sensor, use current potential to carry out tryptophan configuration
Carry out qualitative, use out peak point current to carry out quantitatively, improve susceptiveness and the accuracy of detection L-type tryptophan, minimum permissible
Detect 8.5 × 10-7The L-type tryptophan of mol/L.
Secondly, the Electrochemical Detection L-type color ammonia that the method for the present invention is repaiied as amination graphene quantum dot and beta cyclodextrin
A kind of method of acid, greatly reduces testing cost, simple to operation；
Finally, the present invention one step quickly detects L-type tryptophan, after prepared by three-electrode system sensor, it is only necessary to several points
Clock just can realize a step and detect L-type tryptophan.
Accompanying drawing explanation
Fig. 1 is the differential pulse voltammetry curve of the L-type tryptophan standards solution of variable concentrations in embodiments of the invention 1
Figure；
Fig. 2 is the canonical plotting of L-type tryptophan in embodiments of the invention 1.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is elaborated, after making those of ordinary skill in the art refer to this specification
Can implement according to this.
It should be noted that experimental technique described in following embodiment, if no special instructions, it is conventional method, institute
State reagent and material, if no special instructions, the most commercially obtain.
The term definition that the present invention relates to:
Unless otherwise defined, all technology the most used herein and scientific terminology all have with of the art
Those of ordinary skill is generally understood identical implication.Although can use and described herein in the practice or test of the present invention
Similar or equivalent any method, device and material, but presently describe method for optimizing, device and material.
" working electrode ", also known as Electrode, refers to that studied reaction occurs on this electrode.In general, to work
The basic demand of electrode is: working electrode can be solid, it is also possible to be liquid, and the solid material that can conduct electricity miscellaneous is equal
Can serve as electrode.Reaction that the electrochemical reaction studied will not be occurred because of electrode self and be affected, and can be
Bigger potential areas is measured；Electrode must not react with solvent or electrolyte component；Electrode area should not be too
Greatly, electrode surface should be preferably homogeneous smooth, and can carry out surface cleaning etc. by simple method.Use solid electrode
Time, in order to ensure the repeatability of experiment, it has to be noted that set up suitable electrode pre-treatment step, to ensure oxidoreduction, surface
Pattern and there is not the reproducible state of adsorbing contaminant.
A kind of detect the method for L-type Tryptophan concentration in solution, use three-electrode system by differential pulse voltammetry pair
L-type tryptophan in solution to be measured detects, and obtains L-type tryptophan in solution to be measured according to the linear equation of L-type tryptophan
Concentration, the working electrode in three-electrode system is glass-carbon electrode after amination graphene quantum dot and beta cyclodextrin are modified
The modified glassy carbon electrode arrived.Three-electrode system is made up of working electrode, reference electrode and auxiliary electrode.
The present invention is to use Differential Pulse Voltammetry to detect L-type tryptophan, and Differential Pulse Voltammetry is linearly being swept
Retouching on waveform, the continuous impulse that superposition one amplitude constant and pulsewidth are fixed, in scanning process, base current potential scans from initial potential
Terminate current potential, before potential pulse starts and at the end of carry out current sample, by the difference of the two sample rate current to current potential
Mapping, is DPV curve, is mainly used in electro chemical analysis, reduces the background current caused because of oxidation of impurities reduction reaction, has
Preferably detection sensitivity and lower detectable limit, relative to additive method, described Differential Pulse Voltammetry cost is relatively low, behaviour
Make simple.
The method of L-type Tryptophan concentration in described detection solution, comprises the following steps:
Step one, preparing described working electrode and build three-electrode system, compound concentration is that 7.0～30.0 μm ol/L are many
The standard solution of part L-type tryptophan；
Step 2, use described three-electrode system, utilize differential pulse voltammetry to every part of standard of preparation in step one
Solution detects, and obtains the differential pulse voltammetry curve of every part of standard solution, records every part of standard the most respectively molten
The peak value of current intensity in the differential pulse voltammetry curve of liquid；
Step 3, the current intensity corresponding to differential pulse voltammetry curve of every part of standard solution to obtain in step 2
Peak value, as vertical coordinate, is drawn standard curve with the concentration of every part of standard solution for abscissa and calculates linear equation；
Step 4, use described three-electrode system, utilize differential pulse voltammetry to containing unknown concentration L-type tryptophan
Solution to be measured detects, and obtains the differential pulse voltammetry curve of solution to be measured, by the differential pulse voltammetry curve of solution to be measured
The peak value of corresponding current intensity substitutes in the linear equation obtained in step 3, is calculated L-type tryptophan in solution to be measured
Concentration.
Embodiment 1:
Preparation work electrode: reference electrode, auxiliary electrode and glass-carbon electrode are immersed containing 2mg/mL amination Graphene
In the mixed solution of quantum dot and 2mg/mL beta cyclodextrin, take out after utilizing cyclic voltammetric method to scan described in being drying to obtain
Modified glassy carbon electrode；
Wherein, sweep parameter is set to: initial potential 0V, maximum potential 1V, minimum point position 0V, final current potential 0V, scanning
Speed 0.1V/s, scanning times 100 times, sensitivity 10-4A/V, waiting time are 2s.
Working electrode pretreatment: modified glassy carbon electrode is placed on the polishing cloth upthrow of polishing powder containing granularity 0.3 μm
Glass-carbon electrode, to minute surface, is sequentially placed into methanol, concentration is the H of 0.5mol/L by light subsequently2SO4Solution and ultra-pure water surpass respectively
Sound 30s, and obtain described modified glassy carbon electrode with ultrapure water 1min after each ultrasonic end.
A kind of detect the method for L-type Tryptophan concentration in solution, comprise the following steps:
Step one, preparing described working electrode and build three-electrode system, compound concentration is the 4 of 7.0～30.0 μm ol/L
The standard solution of part L-type tryptophan；
Wherein, the working electrode i.e. preparation method of modified glassy carbon electrode is: after reference electrode, auxiliary electrode and pretreatment
Glass-carbon electrode immerse in the mixed solution containing 2mg/mL amination graphene quantum dot and 2mg/mL beta cyclodextrin, utilization follows
Ring voltammetric method takes out after having scanned and is drying to obtain modified glassy carbon electrode, and sweep parameter is set to: initial potential 0V, the highest electricity
Position 1V, minimum point position 0V, final current potential 0V, sweep speed 0.1V/s, scanning times 100 times, sensitivity 10-4A/V, waiting time
For 2s；
Wherein, in described step one, the standard solution collocation method of L-type tryptophan is: add dense in phosphate buffer solution
Degree is the L-type tryptophan standards solution of 0.01mol/L, prepares L-type Tryptophan concentration respectively and is followed successively by 7.0 × 10-6Mol/L's is molten
Liquid a, 15.0 × 10-5The solution b of mol/L, 1.0 × 10-5The solution c of mol/L, 3.0 × 10-5The solution d of mol/L amounts to 4 marks
Quasi-solution；The pH of wherein said phosphate buffer solution is 7.0, and its concentration is 10mmol/L；
Step 2, use described three-electrode system, utilize differential pulse voltammetry to every part of standard of preparation in step one
Solution detects, and obtains the differential pulse voltammetry curve of every part of standard solution, records every part of standard the most respectively molten
The peak value of current intensity in the differential pulse voltammetry curve of liquid；
Wherein, described step 2 specifically includes following steps:
Step 2.1, described three-electrode system is respectively implanted in described 4 standard solution, is stirred at room temperature 2min, quiet
Only 2min；
Step 2.2, scanning differential pulse collection of illustrative plates, arranging scanning initial potential is-0.1V, and termination current potential is 0.6V, current potential
Increment is 0.004V, Sample Width 0.0167s, and amplitude is 0.05V, pulse width 0.05V, sensitivity 10-4A/V, the waiting time
For 2s；
Step 2.3, measure and record the peak value of current intensity of described 4 standard solution respectively, set up described 4 standards
The differential pulse voltammetry curve of solution, Fig. 1 show the differential pulse voltammetry curve of 4 standard solution.
Step 3, the electricity corresponding to differential pulse voltammetry curve of solution a, b, c, d standard solution to obtain in step 2
The peak value of intensity of flow is as vertical coordinate, and in solution a, b, c, d, the concentration of L-type tryptophan is drawn standard curve for abscissa and counts
Calculate linear equation；Such as, with the peak value of current intensity corresponding to the differential pulse voltammetry curve of solution a as vertical coordinate, with solution a
The concentration of middle L-type tryptophan is abscissa, it may be determined that a point on standard curve, by said method, is changed into by solution a
Solution b, c, d, determine the other three point on standard curve by solution b, c, d, draws standard curve as shown in Figure 2；
Being obtained linear equation by Fig. 2 standard curve: Y=-0.01318-0.00192X, in formula, Y is that current value (I), I are
The peak value of the current intensity that the differential pulse voltammetry curve of every part of standard solution is corresponding, unit is μ A；X is L-type in standard solution
Tryptophan concentration (c), unit is μm ol/L, coefficient R2It is 0.9963.The detection of L-type tryptophan is limited to by modified electrode
8.5×10-7mol/L；
Step 4, use described three-electrode system, utilize differential pulse voltammetry to containing unknown concentration L-type tryptophan
Solution to be measured detects, and obtains the differential pulse voltammetry curve of solution to be measured, calculates the dense of L-type tryptophan in liquid to be detected
Degree: the peak value of the current intensity of solution to be measured corresponding for the differential pulse voltammetry curve of solution to be measured is substituted into linear as Y value
In equation, it is calculated the concentration of L-type tryptophan in liquid to be detected.
Although embodiment of the present invention are disclosed as above, but it is not restricted in description and embodiment listed
Using, it can be applied to various applicable the field of the invention completely, for those skilled in the art, and can be easily
Realizing other amendment, therefore under the general concept limited without departing substantially from claim and equivalency range, the present invention does not limit
In specific details.
Claims (9)
1. detect a method for L-type Tryptophan concentration in solution, wherein, including: build the linear side of L-type tryptophan in advance
Journey, uses the three-electrode system being made up of working electrode, reference electrode and auxiliary electrode by differential pulse voltammetry to be measured
L-type tryptophan in solution carries out detection and obtains relevant parameter, and the linear equation that relevant parameter substitutes into described L-type tryptophan obtains
The concentration of L-type tryptophan in solution to be measured；
Wherein, described working electrode is the modification that glass-carbon electrode obtains after amination graphene quantum dot and beta cyclodextrin are modified
Glass-carbon electrode.
2. the method for L-type Tryptophan concentration in detection solution as claimed in claim 1, wherein, described modified glassy carbon electrode
Preparation method includes:
Reference electrode, auxiliary electrode and glass-carbon electrode are immersed containing 1.5～2.5mg/mL amination graphene quantum dots and 1.5
～in the mixed solution of 2.5mg/mL beta cyclodextrin, take out after utilizing cyclic voltammetric method to scan and be drying to obtain described modification
Glass-carbon electrode；
Wherein, sweep parameter is set to: initial potential 0V, maximum potential 1V, minimum point position 0V, final current potential 0V, sweep speed
0.1V/s, scanning times 100 times, sensitivity 10-4A/V, waiting time are 2s.
3. the method for L-type Tryptophan concentration in detection solution as claimed in claim 2, wherein, builds L-type tryptophan in advance
Linear equation comprises the following steps:
Step one, compound concentration are the standard solution of at least 4 parts of L-type tryptophans of 7.0～30.0 μm ol/L；
Step 2, use described three-electrode system, utilize differential pulse voltammetry that every part of standard solution is detected, obtain every
The differential pulse voltammetry curve of part standard solution, records the differential pulse voltammetry curve of every part of standard solution the most respectively
The peak value of middle current intensity；
Step 3, using the peak value of current intensity corresponding to the differential pulse voltammetry curve of every part of standard solution of gained as vertical seat
Mark, draws standard curve with the concentration of every part of standard solution for abscissa and is calculated the linear equation of L-type tryptophan.
4. the method for L-type Tryptophan concentration in detection solution as claimed in claim 3, wherein, uses described three-electrode system,
Utilize differential pulse voltammetry that the solution to be measured containing unknown concentration L-type tryptophan is detected, obtain the difference of solution to be measured
Sectors rushes volt-ampere curve, finds out the peak parameters of current intensity corresponding to the differential pulse voltammetry curve of solution to be measured, and by institute
State peak value substitute into described in the linear equation of L-type tryptophan, i.e. obtain the concentration of L-type tryptophan in solution to be measured after calculating.
5. the method for L-type Tryptophan concentration in detection solution as claimed in claim 4, wherein, L-type color ammonia in described step one
The standard solution collocation method of acid is: be 6.5～7.5 to pH, and concentration is 10mmol/L phosphate buffer solution and concentration is
The L-type tryptophan solution mixing of 0.01mol/L, preparing L-type Tryptophan concentration the most respectively is 7.0 × 10-6mol/L、10.0×
10-6mol/L、15.0×10-5mol/L、3.0×10-54 standard solution of mol/L.
6. the method for L-type Tryptophan concentration in detection solution as claimed in claim 5, wherein, described step 2 specifically includes
Following steps:
1) described three-electrode system is respectively implanted in described 4 standard solution, under 20～30 DEG C of room temperatures, stirs 1min, static
1min；
2) scanning differential pulse collection of illustrative plates, arranging scanning initial potential is-0.1V, and termination current potential is 0.6V, and current potential increment is
0.004V, Sample Width 0.0167s, amplitude is 0.05V, pulse width 0.05V, sensitivity 10-4A/V, the waiting time is 2s；
3) measure and record the peak value of current intensity of described 4 standard solution respectively, set up the difference of described 4 standard solution
Pulse Voltammetry curve.
7. the method for L-type Tryptophan concentration in detection solution as claimed in claim 1 or 2, wherein, described amination Graphene
Quantum dot obtains after being concentrated by the amination rotated evaporimeter of graphene quantum dot that concentration is 1mg/mL.
8. the method for L-type Tryptophan concentration in detection solution as claimed in claim 2, wherein, described modified glassy carbon electrode makes
With front through pretreatment, pretreatment comprises the following steps:
Modified glassy carbon electrode is placed on the polishing cloth of the polishing powder containing granularity 0.3 μm and is polished to minute surface, subsequently by glass carbon
Electrode is sequentially placed into methanol, concentration is the H of 0.5mol/L2SO4Difference ultrasonic 25～35s in solution and ultra-pure water, and super
Sound obtains the modified glassy carbon electrode of pretreatment with ultrapure water 0.5～1.5min after terminating.
9. the method for L-type Tryptophan concentration in detection solution as claimed in claim 2, wherein, reference electrode is Ag/AgCl electricity
Pole, auxiliary electrode is platinum electrode.
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| CN107764886A (en) * | 2017-10-16 | 2018-03-06 | 山西大学 | The preparation of three-dimensional grapheme nano composite material and the method for chiral Recognition tryptophan |
| CN108362815A (en) * | 2018-01-08 | 2018-08-03 | 哈尔滨理工大学 | A kind of novel L-Trp electrochemical sensor |
| WO2021103682A1 (en) * | 2019-11-25 | 2021-06-03 | 长沙理工大学 | Method for selectively detecting l-tryptophan by means of formaldehyde medium effect |
| CN112946049A (en) * | 2021-03-05 | 2021-06-11 | 西北师范大学 | Preparation and application of beta-cyclodextrin functionalized nitrogen-doped graphene quantum dot chiral composite material |
| CN115728281A (en) * | 2022-12-09 | 2023-03-03 | 山西大学 | Fluorescent probe for detecting L-tryptophan and preparation thereof |
| US11948284B2 (en) | 2020-07-03 | 2024-04-02 | Dalian University Of Technology | 3D modeling method for pore-filling hydrate sediment based on CT image |
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| CN107764886A (en) * | 2017-10-16 | 2018-03-06 | 山西大学 | The preparation of three-dimensional grapheme nano composite material and the method for chiral Recognition tryptophan |
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| CN108362815B (en) * | 2018-01-08 | 2020-11-06 | 哈尔滨理工大学 | Novel L-tryptophan electrochemical sensor |
| WO2021103682A1 (en) * | 2019-11-25 | 2021-06-03 | 长沙理工大学 | Method for selectively detecting l-tryptophan by means of formaldehyde medium effect |
| US11948284B2 (en) | 2020-07-03 | 2024-04-02 | Dalian University Of Technology | 3D modeling method for pore-filling hydrate sediment based on CT image |
| CN112946049A (en) * | 2021-03-05 | 2021-06-11 | 西北师范大学 | Preparation and application of beta-cyclodextrin functionalized nitrogen-doped graphene quantum dot chiral composite material |
| CN115728281A (en) * | 2022-12-09 | 2023-03-03 | 山西大学 | Fluorescent probe for detecting L-tryptophan and preparation thereof |
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