CN106248764B - Real-time visual sharing and integrated imaging nucleic acid electrophoresis device and operation method thereof - Google Patents
Real-time visual sharing and integrated imaging nucleic acid electrophoresis device and operation method thereof Download PDFInfo
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- CN106248764B CN106248764B CN201610631255.7A CN201610631255A CN106248764B CN 106248764 B CN106248764 B CN 106248764B CN 201610631255 A CN201610631255 A CN 201610631255A CN 106248764 B CN106248764 B CN 106248764B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
Abstract
The invention discloses a real-time visual sharing and integrated imaging nucleic acid electrophoresis device, which comprises a base, a top cover and a nucleic acid electrophoresis device, wherein the base is provided with a plurality of through holes; the base is a cavity box body, the nucleic acid electrophoresis device is arranged on the base, and the top cover is arranged above the nucleic acid electrophoresis device; the invention adopts a double-light-source design, mainly adopts a blue-light cold light source detection system without toxic nucleic acid dye, is attached with an ultraviolet light source detection system, and takes care of the current situation that a part of laboratories still use EB dye.
Description
Technical Field
The invention relates to a real-time visual sharing and integrated imaging nucleic acid electrophoresis device, and also relates to an operation method of the real-time visual sharing and integrated imaging nucleic acid electrophoresis device, belonging to the field of molecular biology experiments.
Background
In the conventional experiments in the field of biological research and medical research, such as verification of extraction in plasmids, display of PCR results, and effect evaluation of early sample preparation work of genome sequencing, gel electrophoresis of nucleic acid is the most direct and effective method for providing information such as nucleic acid purity, concentration, fragment size and the like for experimenters, and along with the development of biomedical technology, the gel electrophoresis of DNA and RNA puts forward higher and higher demands on the usability and universality of the gel electrophoresis of DNA and RNA.
The currently widely used nucleic acid development technology is mainly to observe the position of nucleic acid migration in gel and recover nucleic acid by exciting brominated pyridine (EB) embedded in a nucleic acid sample by an ultraviolet light source. The current technology mainly has the following defects: (1) EB is a highly toxic substance, has strong mutagenesis effect, is easy to cause cells to cancerate, has strong volatility and is difficult to degrade, requires a laboratory using EB to set up a special EB pollution area, and simultaneously, laboratory personnel performing nucleic acid electrophoresis and development experiments need to be protected very carefully, otherwise, the laboratory personnel and even the whole laboratory are harmed; (2) the ultraviolet light source has lower illumination intensity. The service life is short, the damage is easy, and the ultraviolet light source also has the harmfulness of DNA damage, so that the damage to experimenters is easy to generate; (3) EB develops needs the professional equipment of ultraviolet formation of image, not only the price is higher but also makes electrophoresis process and development process separate from the technology, has reduced the controllability of electrophoresis experiment, has wasted valuable experimental time. In recent years, various countries around the world are dedicated to research on the possibility of EB substitutes, such as fluorescent nucleic acid dyes like genefinder, sypro green, etc., which employ a visible light source to excite the fluorescent nucleic acid dye and generate a visible light detection source to achieve the purpose of detecting nucleic acid on the premise of ensuring sensitivity. The emergence of these fluorescent nucleic acid dyes has brought an urgent need for the development of fluorescent nucleic acid electrophoresis devices based on non-toxic, low-toxicity fluorescent nucleic acid dyes, and the use of the currently available nucleic acid electrophoresis devices in the market is greatly limited because they cannot be reused.
In addition, the user needs to permanently retain the image of the nucleic acid electrophoresis, which is usually completed by another independent gel imaging device. In operation, the gel containing nucleic acid needs to be transferred from the nucleic acid electrophoresis apparatus to the gel imaging apparatus to complete the imaging process; moreover, another independent computer is generally needed to store the shot gel image; and finally, the images in the computer are downloaded through storage elements such as a U disk and the like, and then are uploaded to the computer of the terminal user, so that the operation is complicated, and the workload is large.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the defects of the prior art and provide a nucleic acid electrophoresis device which can perform real-time imaging, real-time visualization, recovery operation and remote imaging sharing. However, in the market and most laboratories at the present stage, the nucleic acid electrophoresis system containing the EB dye is used all the time, the blue light source system is completely updated in a short time, the consumption is high, and particularly, the popularization difficulty of the laboratory is high. In order to gradually realize the upgrade from the EB dye to the non-toxic blue dye, the invention adopts a double-light-source design, mainly adopts a blue light cold light source detection system of the non-toxic nucleic acid dye, is attached with an ultraviolet light source detection system, and takes care of the current situation that a part of laboratories still use the EB dye.
Another objective of the invention is to provide a method for operating a real-time visual sharing and integral imaging nucleic acid electrophoresis device.
In order to solve the technical problems, the invention provides a real-time visual sharing and integrated imaging nucleic acid electrophoresis device, which comprises a base, a machine top cover and a nucleic acid electrophoresis device, wherein the base is a box body with a cavity, the nucleic acid electrophoresis device is arranged at the top of the base, and the machine top cover is arranged above the nucleic acid electrophoresis device; the nucleic acid electrophoresis device comprises an electrophoresis tank and an observation filter plate, wherein the observation filter plate is arranged above the electrophoresis tank, and further comprises an imaging LED excitation light source, an imaging light source optical filter, a lens optical filter and an imaging lens, the imaging LED excitation light source and the imaging ultraviolet excitation light source are arranged on the lower surface of the top cover, the imaging light source optical filter is arranged between the imaging LED excitation light source and the electrophoresis tank, and the ultraviolet glass is arranged between the imaging ultraviolet excitation light source and the electrophoresis tank; the imaging lens is arranged at the bottom of the base, and the lens optical filter is arranged above the imaging lens; the centers of the imaging LED excitation light source, the imaging light source optical filter, the observation filter plate, the electrophoresis tank, the lens optical filter and the imaging lens are on the same axis, and the centers of the imaging ultraviolet excitation light source, the ultraviolet glass, the observation filter plate, the electrophoresis tank, the lens optical filter and the imaging lens are in coaxial relation by switching of a circuit switch and moving of a machine top cover/the electrophoresis tank.
The LED excitation light source images and observes the filter plate to ensure that only the fluorescence of nucleic acid can penetrate (the penetration wavelength of the filter plate can be changed according to different dyes used), so that the aim of observing nucleic acid electrophoresis in real time is fulfilled; and in order to ensure the unicity of the light source, the imaging LED light source optical filter is close to the LED wavelength peak value through the wavelength peak value, and the white diffusion plate is used for enabling light to be more uniformly diffused, so that the phenomenon that the center of a lamp bead is too bright like a bulb and the gap of the lamp bead is dark is prevented. So that the nucleic acid electrophoresis can be clearly imaged. Through experimental observation and test, set up each light source and light filter in the axis of electrophoresis tank, formation of image LED excitation light source, formation of image light source light filter and camera lens light filter and imaging lens use mutually supporting, can make nucleic acid electrophoresis's formation of image more clear, it is more convenient to use.
The imaging and observation filter plate of the imaging ultraviolet excitation light source is an orange filter plate, the observation filter plate ensures that only the fluorescence of nucleic acid can penetrate, the filter plate can specifically filter the wavelength of less than 500nm, and only the fluorescence of 500nm-550nm emitted by the nucleic acid can penetrate, so that the purpose of observing the nucleic acid electrophoresis in real time is achieved. The ultraviolet-transmitting quartz glass is arranged above the lamp tube, unnecessary stray light is filtered, and only the required excitation light source is reserved to pass through, so that the imaging background is cleaner, and the imaging quality is improved.
The technical scheme of the invention is further defined as further comprising a central processing unit and a touch display screen, wherein the central processing unit is in data connection with the imaging lens and the touch display screen, receives the image collected by the imaging lens and transmits the image to the touch display screen.
Further, the wireless transmitting device is in communication connection with the central processing unit and is Wifi, Bluetooth or 3G.
Further, the imaging lens is a CCD imaging lens or a CMOS imaging lens.
Furthermore, the imaging LED excitation light source and the imaging ultraviolet excitation light source are connected with different power switches.
The invention relates to an operation method of a real-time visual sharing and integral imaging nucleic acid electrophoresis device, which is characterized in that: the method comprises the following steps:
step one, adding fluorescent nucleic acid dye (such as gene color) into melted gel when preparing the gel according to requirements, putting the prepared solidified agarose gel into an electrophoresis tank (5), adding a small amount of electrophoresis liquid, and adding nucleic acid to be analyzed into gel pores;
step two, when the machine top cover 16 is opened, the device is in an observation and recovery state, at the moment, the imaging LED excitation light source (7) is in an open state, the light source of the imaging LED excitation light source (7) passes through the diffusion plate (6) to excite the fluorescent nucleic acid dye combined with the nucleic acid in the electrophoresis tank (5) to emit fluorescence, and through the observation filter plate (4), a user can directly observe the nucleic acid to carry out directional electrophoresis under the action of a fixed electric field, so that the purpose of observing the nucleic acid electrophoresis in real time is achieved, the observation filter plate (4) is lifted, and the user can cut the nucleic acid in the gel, so that the purpose of recovering the target nucleic acid is achieved;
step three, when the observation filter plate (4) is taken off and the top cover (16) is closed, the device is in a blue light imaging, data transmission and sharing state; at the moment, the imaging LED excitation light source (7) and the imaging lens (10) are in an opening state; the user can observe and take pictures of the directional swimming of the nucleic acid in the fixed electric field through the touch display screen (1), and can share the nucleic acid electrophoresis result with collaborators, project units, user groups, guides or related personnel in the global scope by using modern network means such as WeChat groups, QQ groups or cloud storage of image videos and the like at any time;
step four, when the electrophoresis tank (5) is pushed inwards to the bottom and the top cover (16) is pushed to the bottom, the device is in an ultraviolet imaging state, a data transmission state and a sharing state; at the moment, the imaging ultraviolet excitation light source (14) and the imaging lens (10) are in an opening state; the user can observe and take pictures of the directional swimming of the nucleic acid in the fixed electric field through the touch display screen (1), and can share the nucleic acid electrophoresis result with collaborators, project units, user groups, guides or related personnel in the global scope by using modern network means such as WeChat groups, QQ groups or cloud storage of image videos and the like at any time.
The invention has the beneficial effects that: the invention provides a nucleic acid electrophoresis device which can image in real time, is visible in real time, can carry out recovery operation and imaging remote sharing, and adopts a double-light-source design. During imaging, firstly, a fluorescence staining agent combined with nucleic acid is made to emit light by a bottom LED matrix excitation light source, and the real-time imaging purpose of nucleic acid electrophoresis is achieved through an imaging filter plate and a shooting lens; during observation and recovery, the fluorescence staining agent combined with the nucleic acid emits light by the bottom LED matrix excitation light source, and the purposes of real-time observation and nucleic acid recovery are achieved through the observation filter plate; secondly, the fluorescent staining agent combined with the nucleic acid is made to emit light by the bottom imaging ultraviolet excitation light source, and the real-time imaging of the nucleic acid electrophoresis is achieved through the imaging filter plate and the shooting lens; during observation and recovery, the fluorescent staining agent combined with the nucleic acid emits light through the bottom imaging ultraviolet excitation light source, and the purposes of real-time observation and nucleic acid recovery are achieved through the observation filter plate. The purposes of wireless transmission of electrophoresis image results and multi-user global instant sharing are achieved by built-in WiFi or Bluetooth or 3G functions and modern network means such as WeChat groups or QQ groups or cloud storage of image videos. The invention adopts double light sources to observe and recover the fluorescence nucleic acid electrophoresis in real time, and the fluorescence nucleic acid electrophoresis imaging and instant nucleic acid electrophoresis result are shared globally, thereby not only meeting the gradual upgrade and update of the EB dye to the nontoxic blue dye, but also taking care of the current situation that a part of people still use the EB dye, effectively combining the advanced technologies of fluorescence nucleic acid electrophoresis, traditional nucleic acid electrophoresis, instant imaging, image wireless transmission, data global sharing and the like, filling the domestic market blank, and being widely applied to the fields of biology, medicine, agriculture and the like which need molecular biology experiments.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
FIG. 2 is a schematic diagram of the blue light cold light source detection system using non-toxic nucleic acid dye according to the present invention.
FIG. 3 is a schematic diagram of the structure of the detection system using the UV light source according to the present invention.
In the figure: 1-a touch display screen; 2-a lens filter; 3-a cooling fan; 4-observation filter plate; 5-an electrophoresis tank; 6-a diffusion plate; 7-imaging LED excitation light source; 8-LED light source switch; 9-ultraviolet light source switch; 10-an imaging lens; 11-an electrophoretic switch; 12-a metal contact; 13-ultraviolet glass; 14-imaging ultraviolet excitation light source; 15-a base; 16-a machine top cover; 17-sliding track.
Detailed Description
As shown in FIGS. 1-3, the present invention relates to a real-time visual sharing and integral imaging nucleic acid electrophoresis apparatus, which comprises a base 15, a top cover 16 and a nucleic acid electrophoresis apparatus; the base 15 is a cavity box body, the nucleic acid electrophoresis device is arranged on the base 15, and the top cover 16 is arranged above the nucleic acid electrophoresis device; the nucleic acid electrophoresis device comprises an electrophoresis tank 5 and an observation filter plate 4, wherein the observation filter plate 4 is arranged above the electrophoresis tank 5, the nucleic acid electrophoresis device also comprises an imaging LED excitation light source 7, a diffusion plate 6, an imaging ultraviolet excitation light source 14, ultraviolet glass 13, a lens filter 2, an imaging lens 10 and a sliding guide rail 17, the imaging LED excitation light source 7 is arranged on the lower surface of the base 15, and the diffusion plate 6 is arranged below the electrophoresis tank 5; the imaging ultraviolet excitation light source 14 is arranged on the upper surface of the base 15, the ultraviolet glass 13 is arranged on the upper surface of the imaging ultraviolet excitation light source 14, the imaging lens 10 is arranged above the electrophoresis tank 5, the lens optical filter 2 is arranged below the imaging lens 10, the sliding guide rail 17 is arranged below the top cover 16, the electrophoresis tank 5 can slide along the sliding guide rail 17, and the electrophoresis tank 5 is communicated with a power supply through the metal contact 12. The distance between the diffusion plate 6 and the imaging LED excitation light source 7 is 10-15cm, and the distance between the imaging lens 10 and the electrophoresis tank 5 can be selected according to the size of the electrophoresis colloid and the requirements of the overall dimension of the product.
As shown in fig. 2, when the blue-light cold-light detection system using non-toxic nucleic acid dye is used, the centers of the imaging LED excitation light source 7, the diffusion plate 6, the observation filter plate 4, the electrophoresis tank 5, the lens filter 2 and the imaging lens 10 are on the same axis.
As shown in fig. 3, when the uv light source detection system is used, the centers of the imaging uv excitation light source 14, the uv glass 13, the observation filter plate 4, the electrophoresis tank 5, the lens filter 2, and the imaging lens 10 are on the same axis.
The invention further comprises a central processing unit and a touch display screen 1, wherein the central processing unit is in data connection with the imaging lens 10 and the touch display screen 1, receives the image collected by the imaging lens 10 and transmits the image to the touch display screen 1. The wireless transmitting device is in communication connection with the central processing unit and is Wifi, Bluetooth or 3G. The imaging lens 10 is a CCD imaging lens or a CMOS imaging lens. The imaging excitation light source can be an imaging LED excitation light source 7 or/and an imaging ultraviolet excitation light source 14, the two light sources can be arranged side by side at the same time and switched by a circuit switch, and the coaxial relation between the imaging lens and the electrophoresis tank 5 and the positions of the light sources is kept by moving the position of the top cover 16/the electrophoresis tank 5.
The base 15 of the invention is a box body with a cavity, the box body is rectangular, and the top end of the box body is provided with a top cover 16.
The nucleic acid electrophoresis device is arranged at the top of the base 15 and is a slot-in type micro nucleic acid electrophoresis device which comprises an electrophoresis slot 5 and an observation filter plate 4, wherein the observation filter plate 4 is arranged above the electrophoresis slot 5. The micro nucleic acid electrophoresis device can be a nucleic acid electrophoresis device commonly used in the art, and the structure of the micro nucleic acid electrophoresis device is not described in detail in the present invention.
The real-time visual sharing and integral imaging nucleic acid electrophoresis device also comprises a real-time imaging part, a real-time observation part and an imaging sharing part, and the three parts are described in detail below.
The real-time imaging part comprises two parts: the device comprises an imaging LED excitation light source 7, a diffusion plate 6, a lens filter 2 and an imaging lens 10. The imaging LED excitation light source 7 is arranged on the base 15, the lens filter 2 is arranged between the imaging LED excitation light source 7 and the imaging lens 10, and the diffusion plate 6 is arranged above the LED excitation light source 7. The imaging lens 10 is arranged at the top of the top cover 16, and the lens filter 2 is arranged below the imaging lens 10; the centers of the imaging LED excitation light source 7, the diffusion plate 6, the lens filter 2 and the imaging lens 10 are on the same axis. The imaging lens 10 is a CCD imaging lens or a CMOS imaging lens. The imaging device comprises an imaging ultraviolet excitation light source 14, ultraviolet glass 13, a lens filter 2 and an imaging lens 10. The imaging ultraviolet excitation light source 14 is arranged on the base 15, the lens filter 2 is arranged between the imaging ultraviolet excitation light source 14 and the imaging lens 10, and the ultraviolet glass 13 is arranged above the imaging ultraviolet excitation light source 14. The imaging lens 10 is arranged at the top of the top cover 16, and the lens filter 2 is arranged below the imaging lens 10; the centers of the imaging ultraviolet excitation light source 14, the ultraviolet glass 13, the lens filter 2 and the imaging lens 10 are on the same axis. The imaging lens 10 is a CCD imaging lens or a CMOS imaging lens.
The real-time observation and recovery part comprises an LED excitation light source 7 and an observation filter plate 4, the observation LED excitation light source is arranged on the base 15, and the observation filter plate 4 is arranged above the electrophoresis tank 5. An imaging ultraviolet excitation light source 14 and an observation filter plate 4, wherein the imaging ultraviolet excitation light source is arranged on the base 15, and the observation filter plate 4 is arranged above the electrophoresis tank 5.
The imaging sharing part comprises a central processing unit and a touch display screen 1, wherein the central processing unit is in data connection with the imaging lens 10 and the touch display screen 1, receives an image collected by the imaging lens 10 and transmits the image to the touch display screen 1. The touch display screen 1 is arranged at the top end of the top cover 16, so that the touch display screen is convenient to observe and use.
Meanwhile, the imaging sharing part further comprises a wireless transmitting device in communication connection with the central processing unit, and the wireless transmitting device is Wifi, Bluetooth or 3G. The purposes of wireless transmission of electrophoresis results and multi-user global instant sharing are achieved by using modern network means such as WeChat groups or QQ groups or cloud storage of image videos.
In addition, the imaging LED excitation light source 7 and the imaging ultraviolet excitation light source 14 are connected with different power switches to be controlled independently, and the use is convenient.
The operation method of the real-time visible and imaging shared nucleic acid electrophoresis device comprises the following steps:
(1) adding fluorescent nucleic acid dye (such as gene color) into molten gel during preparation of gel, placing the prepared solidified agarose gel into an electrophoresis tank, adding a small amount of electrophoresis solution, and adding nucleic acid to be analyzed into gel pores.
(2) When the bonnet 16 is opened, the apparatus is in a viewing and retrieval position. At this moment, the imaging LED excitation light source 7 is in an open state, the light source of the LED light source 7 passes through the diffusion plate 6, the fluorescent nucleic acid dye combined with the nucleic acid in the electrophoresis tank 5 is excited to emit fluorescence, and a user can directly observe the nucleic acid to perform directional electrophoresis under the action of a fixed electric field through the observation filter plate 4, so that the purpose of observing the nucleic acid electrophoresis in real time is achieved. The observation filter plate 4 is lifted, and the user can cut the nucleic acid in the gel, so that the purpose of recovering the target nucleic acid is achieved.
(3) When the viewing filter 4 is removed and the top cover 16 is closed, the device is in blue imaging, data transmission and sharing mode. At this time, the imaging LED excitation light source 7 and the imaging lens 10 are in an on state. The user can observe and photograph the directional swimming of the nucleic acid in the fixed electric field by touching the display screen 1, and can share the nucleic acid electrophoresis result with collaborators, project units, user groups, guides or related personnel in the global scope by using modern network means such as WeChat group or QQ group or cloud storage of image video and the like at any time.
(4) When the electrophoresis tank 5 is pushed inwards to the bottom and the top cover is pushed together to the bottom position, the device is in the ultraviolet imaging, data transmission and sharing state. At this time, the imaging ultraviolet excitation light source 14 and the imaging lens 10 are in an on state. The user can observe and photograph the directional swimming of the nucleic acid in the fixed electric field by touching the display screen 1, and can share the nucleic acid electrophoresis result with collaborators, project units, user groups, guides or related personnel in the global scope by using modern network means such as WeChat group or QQ group or cloud storage of image video and the like at any time.
In addition to the above embodiments, the present invention may have other embodiments. All technical solutions formed by adopting equivalent substitutions or equivalent transformations fall within the protection scope of the claims of the present invention.
Claims (7)
1. A real-time visual sharing and integrated imaging nucleic acid electrophoresis device comprises a base (15), a top cover (16) and a nucleic acid electrophoresis device; the base (15) is a cavity box body, the nucleic acid electrophoresis device is arranged on the base (15), and the top cover (16) is arranged above the nucleic acid electrophoresis device; the method is characterized in that: the nucleic acid electrophoresis device comprises an electrophoresis tank (5) and an observation filter plate (4), wherein the observation filter plate (4) is arranged above the electrophoresis tank (5), the nucleic acid electrophoresis device also comprises an imaging LED excitation light source (7), a diffusion plate (6), an imaging ultraviolet excitation light source (14), ultraviolet glass (13), a lens filter (2), an imaging lens (10) and a sliding track (17), the imaging LED excitation light source (7) is arranged on the lower surface of a base (15), and the diffusion plate (6) is arranged below the electrophoresis tank (5); the imaging ultraviolet excitation light source (14) is arranged on the upper surface of the base (15), the ultraviolet glass (13) is arranged on the upper surface of the imaging ultraviolet excitation light source (14), the imaging lens (10) is arranged above the electrophoresis tank (5), and the lens optical filter (2) is arranged below the imaging lens (10); the sliding rail (17) is arranged below the top cover (16), the electrophoresis tank (5) can slide along the sliding rail (17), and the electrophoresis tank (5) is communicated with a power supply through a metal contact (12); the distance between the diffusion plate (6) and the imaging LED excitation light source (7) is 10-15cm, and different focal lengths and distances are selected according to the size of the electrophoresis colloid and the requirements of the product overall dimension between the imaging lens (10) and the electrophoresis tank (5).
2. The apparatus according to claim 1, wherein the apparatus comprises: when the blue light cold light source detection system using the non-toxic nucleic acid dye is used, the centers of the imaging LED excitation light source (7), the diffusion plate (6), the observation filter plate (4), the electrophoresis tank (5), the lens filter (2) and the imaging lens (10) are on the same axis.
3. The apparatus according to claim 1, wherein the apparatus comprises: when the ultraviolet light source detection system is used, the centers of the imaging ultraviolet excitation light source (14), the ultraviolet glass (13), the observation filter plate (4), the electrophoresis tank (5), the lens filter (2) and the imaging lens (10) are on the same axis.
4. The apparatus according to claim 1, wherein the apparatus comprises: the imaging lens is characterized by further comprising a central processing unit and a touch display screen (1), wherein the central processing unit is in data connection with the imaging lens (10) and the touch display screen, receives an image collected by the imaging lens (10), and transmits the image to the touch display screen (1).
5. The apparatus according to claim 4, wherein the apparatus comprises: the wireless transmitting device is in communication connection with the central processing unit and is Wifi, Bluetooth or 3G.
6. The apparatus according to claim 1, wherein the apparatus comprises: the imaging lens (10) is a CCD imaging lens or a CMOS imaging lens.
7. The apparatus according to claim 1, wherein the apparatus comprises: the imaging excitation light source is an imaging LED excitation light source (7) and an imaging ultraviolet excitation light source (14), the two light sources are arranged side by side at the same time and switched by a circuit switch, and the positions of the top cover (16) and the electrophoresis tank (5) are moved to keep the coaxial relation between the imaging lens and the electrophoresis tank (5) and the positions of the light sources.
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| DE59915204D1 (en) * | 1998-08-28 | 2010-10-28 | Febit Holding Gmbh | PROCESS FOR THE PRODUCTION OF BIOCHEMICAL REACTION CARRIER |
| CN2348410Y (en) * | 1999-01-27 | 1999-11-10 | 中国人民解放军北京军区总医院 | White light and ultraviolet transmission instrument for taking photos |
| CN2560943Y (en) * | 2002-08-27 | 2003-07-16 | 郭晏海 | Gel image analyser |
| CN101865876A (en) * | 2009-04-15 | 2010-10-20 | 上海培清科技有限公司 | Fully automatic molecular electrophoresis gel imaging analyser |
| US20130112562A1 (en) * | 2011-11-04 | 2013-05-09 | Salubris, Inc. | Real-time observable electrophoresis system which does not require any kind of light filter |
| CN103713033A (en) * | 2013-12-31 | 2014-04-09 | 南京山诺生物科技有限公司 | Real-time, visible and image-shared nucleic acid electrophoresis device |
| CN206339518U (en) * | 2016-08-03 | 2017-07-18 | 南京山诺生物科技有限公司 | The nucleic acid electrophoresis apptss that a kind of Real time visible is shared and one is imaged |
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