CN106248464B - Cell climbing sheet efficient dyeing device - Google Patents
Cell climbing sheet efficient dyeing device Download PDFInfo
- Publication number
- CN106248464B CN106248464B CN201610800499.3A CN201610800499A CN106248464B CN 106248464 B CN106248464 B CN 106248464B CN 201610800499 A CN201610800499 A CN 201610800499A CN 106248464 B CN106248464 B CN 106248464B
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- cell
- staining
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- dyeing
- slide
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- 230000009194 climbing Effects 0.000 title claims abstract description 28
- 238000004043 dyeing Methods 0.000 title claims abstract description 26
- 238000010186 staining Methods 0.000 claims abstract description 25
- 238000007789 sealing Methods 0.000 claims abstract description 15
- 238000002474 experimental method Methods 0.000 abstract description 8
- 238000001514 detection method Methods 0.000 abstract description 4
- 239000012192 staining solution Substances 0.000 abstract description 3
- 239000012530 fluid Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003125 immunofluorescent labeling Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 230000004938 stress stimulation Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to the technical field of cell biology, in particular to a cell slide high-efficiency dyeing device which comprises a porous dyeing plate and a cell slide assembly platform; the porous dyeing plate comprises a plurality of dyeing through holes and a sealing ring groove, a sealing ring is arranged in the sealing ring groove, and four table-board screw holes are formed in four corners of the porous dyeing plate; cell climbing piece assembly platform includes that the cell climbing piece is placed the platform, and there is a microscope observation hole at cell climbing piece placement platform center, and cell climbing piece assembly platform four corners is provided with four mesa screws. Different staining solutions or labeled antibodies can be added into a plurality of staining through holes of the porous staining plate to respectively label corresponding areas on the cell slide, and after staining is finished, the staining condition can be detected through a microscope observation hole. The cell slide staining device has the advantages of simple structure, low cost and strong operability, and can stain a plurality of areas of a large-size cell slide respectively, thereby improving the utilization rate of cell samples, improving the result detection efficiency and accuracy, effectively improving the experiment efficiency and reducing the experiment cost.
Description
[ technical field ] A method for producing a semiconductor device
The invention relates to the technical field of cell biology, in particular to a cell climbing sheet efficient dyeing device.
[ background of the invention ]
In cell biology research, a cell climbing method is often used for pretreating cells, then immunofluorescence staining is carried out, cell life phenomena are observed, and cell life activity rules are researched. Under specific conditions, such as when a parallel flat plate flow cavity is used for applying fluid shear stress stimulation to cells, the used slide has large size (75 mm multiplied by 25 mm), antibody marking and washing are required to be carried out on the whole slide during dyeing, adherent cells are easy to fall off in the operation process, and low experiment efficiency and high experiment cost are caused. By using the small-sized climbing piece, the small-sized climbing piece is easy to shake or wash away under the action of the fluid shearing force, and the problem cannot be solved.
Meanwhile, the cell slide is large in size, if only one staining marker is carried out, the sample can be greatly wasted, multiple staining markers are simultaneously and respectively realized on the large-size slide, and the utilization rate of the cell sample and the detection efficiency of an experimental result can be improved.
At present, researchers lack an effective technical means suitable for large-size cell slide multi-staining labeling (non-co-staining).
[ summary of the invention ]
The invention aims to provide the efficient cell slide staining device which is simple in structure, reasonable in design and convenient to use, and aims to overcome the defects and shortcomings of the prior art.
The invention relates to a cell slide high-efficiency dyeing device, which comprises a porous dyeing plate and a cell slide assembly platform;
the porous dyeing plate comprises a plurality of dyeing through holes and a sealing ring groove, a sealing ring is arranged in the sealing ring groove, and four table-board screw holes are formed in four corners of the porous dyeing plate;
cell climbing piece assembly platform includes that the cell climbing piece is placed the platform, and there is a microscope observation hole at cell climbing piece placement platform center, and cell climbing piece assembly platform four corners is provided with four mesa screws.
In the present invention, the cell slide 31 is placed on the cell slide placing table of the cell slide assembling platform 2.
In the invention, the cell climbing piece assembly platform provided with the cell climbing piece is tightly attached to the porous dyeing plate provided with the sealing ring through the table-board screw hole by using the fixing bolt, so that the connection is firm.
In the invention, different staining solutions or labeled antibodies can be added into a plurality of staining through holes of the porous staining plate to respectively label corresponding areas on the cell slide, and after staining is finished, the staining condition can be detected through a microscope observation hole.
After adopting above structure, the beneficial effect of the invention is: the efficient staining device for the cell slide has the advantages of simple structure, low cost and strong operability, and can stain a plurality of areas of a large-size cell slide respectively, so that the utilization rate of cell samples is improved, the result detection efficiency and accuracy are improved, the experiment efficiency is effectively improved, and the experiment cost is reduced.
[ description of the drawings ]
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, and are not to be considered limiting of the invention, in which:
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the structure of a porous dyeing plate according to the present invention;
FIG. 3 is a schematic structural diagram of the cell slide assembling platform of the present invention.
Description of reference numerals:
1. a porous staining plate; 2. a cell slide assembly platform; 3. a cell slide placing table; 31. cell climbing sheets; 4. dyeing the through holes; 5. observing the hole through a microscope; 6. a seal ring groove; 7. and a screw hole.
[ detailed description ] embodiments
The present invention will now be described in detail with reference to the drawings and specific embodiments, wherein the exemplary embodiments and descriptions are provided only for the purpose of illustrating the present invention and are not to be construed as limiting the present invention.
As shown in fig. 1-3, the efficient staining apparatus for cell slide according to the present embodiment comprises a porous staining plate 1 and a cell slide assembly platform 2;
the porous dyeing plate 1 comprises a plurality of dyeing through holes 4 and a sealing ring groove 6, a sealing ring is placed in the sealing ring groove 6, and four table-board screw holes 7 are formed in four corners of the porous dyeing plate 1;
cell climbing piece assembly platform 2 includes that cell climbing piece places platform 3, and cell climbing piece places platform 3 center and has microscope observation hole 5, and 2 four corners of cell climbing piece assembly platform are provided with four mesa screws 7.
In the present invention, the cell slide 31 is placed on the cell slide placing table 3 of the cell slide assembling platform 2.
In the invention, the cell climbing piece assembly platform 2 provided with the cell climbing piece 31 is tightly attached to the porous dyeing plate 1 provided with the sealing ring through the table-board screw hole 7 by using a fixing bolt, so that the connection is firm.
In the invention, different staining solutions or labeled antibodies can be added into a plurality of staining through holes 4 of the porous staining plate 1 to respectively label corresponding areas on the cell slide 31, and after staining is finished, the staining condition can be detected through a microscope observation hole 5.
The efficient staining device for the cell slide has the advantages of being simple in structure, low in cost and strong in operability, and can be used for staining a plurality of areas of a large-size cell slide respectively, so that the utilization rate of cell samples is improved, the result detection efficiency and accuracy are improved, the experiment efficiency is effectively improved, and the experiment cost is reduced.
The above description is only a preferred embodiment of the present invention, and all equivalent changes or modifications of the structure, characteristics and principles described in the present invention are included in the scope of the present invention.
Claims (1)
1. The utility model provides a high-efficient dyeing apparatus of cell slide which characterized in that: it comprises a porous staining plate and a cell slide assembling platform;
the porous dyeing plate comprises a plurality of dyeing through holes and a sealing ring groove, a sealing ring is arranged in the sealing ring groove, and four table-board screw holes are formed in four corners of the porous dyeing plate;
the cell slide assembling platform comprises a cell slide placing table, a microscope observation hole is formed in the center of the cell slide placing table, and four table-board screw holes are formed in four corners of the cell slide assembling platform; a cell climbing sheet is placed on a cell climbing sheet placing table of the cell climbing sheet assembling platform; the cell climbing piece assembly platform provided with the cell climbing piece is tightly attached to the porous dyeing plate provided with the sealing ring through the table-board screw hole by utilizing the fixing bolt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610800499.3A CN106248464B (en) | 2016-09-02 | 2016-09-02 | Cell climbing sheet efficient dyeing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610800499.3A CN106248464B (en) | 2016-09-02 | 2016-09-02 | Cell climbing sheet efficient dyeing device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106248464A CN106248464A (en) | 2016-12-21 |
| CN106248464B true CN106248464B (en) | 2020-06-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610800499.3A Active CN106248464B (en) | 2016-09-02 | 2016-09-02 | Cell climbing sheet efficient dyeing device |
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| Country | Link |
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| CN (1) | CN106248464B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108088726B (en) * | 2018-02-08 | 2020-12-22 | 爱威科技股份有限公司 | Multifunctional optional dyeing device |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100261159A1 (en) * | 2000-10-10 | 2010-10-14 | Robert Hess | Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof |
| CN1920559A (en) * | 2005-08-24 | 2007-02-28 | 赵翀 | Cellular biological technique, reagent kits and preparation device |
| CN201737950U (en) * | 2010-07-29 | 2011-02-09 | 上海中医药大学 | Special experimental apparatus for cell growing on cover glass |
| CN102539740A (en) * | 2010-12-29 | 2012-07-04 | 河北省健海生物芯片技术有限责任公司 | Protein chip and method for detecting 80 kinds of autoantibodies simultaneously |
| CN202201884U (en) * | 2011-08-02 | 2012-04-25 | 王开道 | Kit for cell biotechnology |
| CN202322856U (en) * | 2011-11-14 | 2012-07-11 | 江苏百奥特医疗仪器科技有限公司 | Cell culture device for high-flux high-resolution immunofluorescent staining |
| CN202322855U (en) * | 2011-11-14 | 2012-07-11 | 江苏百奥特医疗仪器科技有限公司 | Cell culture device for immunofluorescence staining |
| CN203324275U (en) * | 2013-05-13 | 2013-12-04 | 湖南光琇高新生命科技有限公司 | Immunochemical staining water-blocking device, cell culture dish and cell culture plate |
| CN104101701B (en) * | 2014-07-15 | 2015-10-28 | 上海中医药大学附属曙光医院 | High efficiency cell creep plate ImmunohistochemistryMethods Methods |
| CN203999635U (en) * | 2014-07-17 | 2014-12-10 | 天津医科大学总医院 | Detachable cell climbing sheet culturing plate |
| CN106092703B (en) * | 2014-09-26 | 2018-07-17 | 南通大学 | Application of the easy to operate groupization pen in the detection of cell climbing sheet immunohistochemistry |
| CN205038401U (en) * | 2015-09-02 | 2016-02-17 | 李宏 | Special slide glass convenient to under a microscope observes cell slide |
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| CN106248464A (en) | 2016-12-21 |
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