CN106244471A - The fungal bacterial strain of a kind of resistance to heavy metal Hg and application thereof - Google Patents
The fungal bacterial strain of a kind of resistance to heavy metal Hg and application thereof Download PDFInfo
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- CN106244471A CN106244471A CN201610876134.9A CN201610876134A CN106244471A CN 106244471 A CN106244471 A CN 106244471A CN 201610876134 A CN201610876134 A CN 201610876134A CN 106244471 A CN106244471 A CN 106244471A
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- bacterial strain
- heavy metal
- strain
- hydrargyrum
- aureobasidium pullulans
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- 230000001580 bacterial effect Effects 0.000 title claims abstract description 44
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 32
- 230000002538 fungal effect Effects 0.000 title description 4
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims abstract description 39
- 229920001218 Pullulan Polymers 0.000 claims abstract description 20
- 235000019423 pullulan Nutrition 0.000 claims abstract description 20
- 241000894006 Bacteria Species 0.000 claims abstract description 16
- 241000223678 Aureobasidium pullulans Species 0.000 claims abstract description 7
- 238000001179 sorption measurement Methods 0.000 abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 10
- 238000004321 preservation Methods 0.000 abstract description 6
- 239000002689 soil Substances 0.000 abstract description 4
- 231100000219 mutagenic Toxicity 0.000 abstract 1
- 230000003505 mutagenic effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 11
- 238000000855 fermentation Methods 0.000 description 9
- 230000004151 fermentation Effects 0.000 description 9
- 229910052753 mercury Inorganic materials 0.000 description 8
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 6
- 238000012216 screening Methods 0.000 description 6
- 230000035772 mutation Effects 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 239000000356 contaminant Substances 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 230000035479 physiological effects, processes and functions Effects 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
- 230000009514 concussion Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000002962 chemical mutagen Substances 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 208000008763 Mercury poisoning Diseases 0.000 description 1
- 206010027439 Metal poisoning Diseases 0.000 description 1
- 208000030527 Minamata disease Diseases 0.000 description 1
- 208000016285 Movement disease Diseases 0.000 description 1
- VZUNGTLZRAYYDE-UHFFFAOYSA-N N-methyl-N'-nitro-N-nitrosoguanidine Chemical compound O=NN(C)C(=N)N[N+]([O-])=O VZUNGTLZRAYYDE-UHFFFAOYSA-N 0.000 description 1
- 208000009507 Nervous System Mercury Poisoning Diseases 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 description 1
- 241000283898 Ovis Species 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 206010044565 Tremor Diseases 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000009388 chemical precipitation Methods 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- HAWPXGHAZFHHAD-UHFFFAOYSA-N mechlorethamine Chemical compound ClCCN(C)CCCl HAWPXGHAZFHHAD-UHFFFAOYSA-N 0.000 description 1
- 229960004961 mechlorethamine Drugs 0.000 description 1
- 229960002523 mercuric chloride Drugs 0.000 description 1
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 1
- -1 mercury ions Chemical class 0.000 description 1
- BQPIGGFYSBELGY-UHFFFAOYSA-N mercury(2+) Chemical compound [Hg+2] BQPIGGFYSBELGY-UHFFFAOYSA-N 0.000 description 1
- 238000005272 metallurgy Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000003387 muscular Effects 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 235000010288 sodium nitrite Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
Abstract
The invention discloses a strain Aureobasidium pullulans bacteria strainAureobasidium pullulans F134M, this bacterial strain is separated, screen, identify, mutagenic obtained, has been preserved in Guangdong Province's Culture Collection preservation, and deposit number is: GDMCC No:60070.The aureobasidium pullulans of the present invention can tolerate and Adsorption of Heavy Metals hydrargyrum, may be used for the improvement of water body and the soil etc. of heavy metal Hg pollution.
Description
Technical field
The invention belongs to biological technical field or technical field of environmental management, relate to administer what heavy metal Hg polluted
Bacterial strain, and obtain method and the application in heavy metal Hg pollution control of this bacterial strain of this bacterial strain.
Background technology
Mercury and mercuric compounds is the important raw material of industry, is widely used in metallurgy, chemical industry, electronics, paint, plastics, system
The field such as medicine, pesticide, but after a large amount of use mercury and mercuric compounds, mercurous material can enter in environment by all means, draws
Play serious problem of environmental pollution.Hydrargyrum heavy metal salt is difficult to remove, and therefore can exist for a long time in natural environment, and can lead to
Cross food chain enrichment and jeopardize human health, such as " minamata disease " that occur at Japan's Minamata.Chronic mercury poisoning there will be headache, spirit
Blunt, numb limbs and tense tendons and the symptom such as pain, dizziness, movement disorder, muscular tremor.
The administering method of mercury pollution mainly have chemical precipitation method, metal deoxidization, active carbon adsorption, ion exchange,
Filtration, electrolysis, Pilus Caprae seu Ovis absorption process, microbial method etc..Wherein, microbial method is the most emerging method, has without two
The advantages such as secondary pollution, applied widely, low cost.
Chinese patent CN 105502686 A usesE.coliDimercurion in sewage is changed into hydrargyri subchloridum sink
Deposit is at the bottom, thus reduces the mercury content in water, and the hydrargyrum concentration that can administer is 200 ug/L；Chinese patent CN
104560736 A use fungusFusarium oxysporumAs hydrargyrum in adsorbent sewage, and then reduce in water body
Mercury content, the hydrargyrum concentration that can administer is 50 mg/ml.At present, the hydrargyrum concentration in the sewage that microbial method is administered is the most relatively low, therefore
The microorganism finding a kind of high resistance to hydrargyrum performance is significant for the removal of high concentration mercury ion.
Summary of the invention
The technical purpose of the present invention is tolerance the aureobasidium pullulans bacterium of Adsorption of Heavy Metals hydrargyrum providing a strain novel
Strain, is used for removing heavy metal Hg.
Concrete, technical scheme is as follows:
The Aureobasidium pullulans bacteria strain of the one resistance to heavy metal Hg of strain, wherein, this strain classification is namedAureobasidium pullulans F134M, is preserved in Guangdong Province's Culture Collection preservation, and deposit number is: GDMCC NO:60070.
Wherein, the bacterial strain of the present invention is through original strainAureobasidium pullulans F13 is lured by chemistry
Become and obtain.
Method of the present invention, wherein, original strainAureobasidium pullulans F13 screening, identify
Concrete operation step is:
(1) carry out gradient dilution after being dissolved in physiological saline solution by the soil from Xinjiang region heavy metal pollution, choose
10-2、10-3、10-4Diluent is respectively coated on the isolation medium flat board containing heavy metal, each gradient 3 repetition, puts 28 DEG C
Cultivate.The streak inoculation respectively of picking shape, size, color etc. are different after growing bacterium colony bacterium colony in corresponding flat board, until
Fall without miscellaneous bacteria.
(2) the F13 bacterial strain determined after cultivating screening cultivates 6-7d in 28 DEG C, observes form, and the bacterium colony initial stage is white, viscous
Thick, the later stage transfers brownish black to, protruding.F13 bacterial strain is carried out system Physiology and biochemistry qualification, through physiology with reference to " Fungal identification handbook "
Biochemistry detection determines that F13 bacterial strain isAureobasidiumBelong to bacterium.
(3) the LSU rDNA sequence of F13 bacterial strain is obtained by PCR, through sequencing and BLAST homology alignment and evolution
Analyze, result show F13 bacterial strain withAureobasidium pullulans var. subglaciale EXF-2480LSU
The homology of rDNA is 100%, therefore by F13 Strain Designation isAureobasidium pullulans F13。
Method of the present invention, wherein, mutation original strainAureobasidium pullulans F13 obtainsAureobasidium pullulans The concrete operation step of F134M is:
(1) by starting strainAureobasidium pullulans F13 carries out chemomorphosis, obtains mutant, wherein, changes
Learn the condition of mutation be the concentration of chemical mutagen be 0.05-0.2 mol/L, mutation time is 20-60min.
(2) mutant strain of step (1) gained is coated contain respectively high concentration heavy metal Hg selectivity solid training
Support base flat board, cultivate, obtain the aureobasidium pullulans of enduring high-concentration heavy metal Hg.
(3) investigating the resistant stability of the bacterial strain of the heavy metal tolerance of step (2) gained, a final selected strain resists
The bacterial strain that property is stable, namedAureobasidium pullulans F134M。
(4) wherein, chemical mutagen includes N-methyl-N '-nitro-N nitrosoguanidine, chlormethine, nitrous acid etc..
Bacterial strain of the present invention is resistant to heavy metal Hg, and the highest tolerable concentration to hydrargyrum is 700 mg/L.
The bacterial strain heavy metal hydrargyrum of the present invention has adsorption, and as hydrargyrum concentration≤200 mg/L, adsorption rate all can reach
To 99%.
The bacterial strain of the present invention application in water body and heavy metal pollution of soil are administered.
Concrete, the bacterial strain of resistance to heavy metal HgAureobasidium pullulans F134M is in terms of Adsorption of Heavy Metals hydrargyrum
Application process be:
WillAureobasidium pullulans F134M is inoculated in fermentation medium, at 28 DEG C, rotating speed 150-200rpm bar
Under part, 6-7d is cultivated in concussion, obtains fermentation liquid.Fermentation liquid is centrifuged, removes culture medium, it is thus achieved that thalline.Thalline is placed in and contains respectively
Have in the heavy metal-containing polluted water of hydrargyrum, adsorb 24-72h.
The beneficial effects of the present invention is:
The mutant that the present invention obtainsAureobasidium pullulans F134M, it is possible to heavy metal tolerance hydrargyrum is wherein, right
The highest tolerable concentration of hydrargyrum is 700 mg/L, improves 40% than original strain.
The mutant that the present invention obtainsAureobasidium pullulans F134M bacterial strain heavy metal hydrargyrum has absorption
Effect, as hydrargyrum concentration≤200 mg/L, adsorption rate all can reach 99.9%.
Therefore, the bacterial strain of the present invention can be used for, in the pollution control of heavy metal Hg, having effect more more preferable than existing bacterial strain.
The explanation of biomaterial: strain of the present invention, its Classification And Nomenclature isAureobasidium pullulans
F134M, has been preserved in Guangdong Province's Culture Collection (GDMCC), and deposit number is: GDMCC No:60070, preservation
Date is: on 08 29th, 2016, preservation address was: 5th floors, No. 59 building of compound, Xianlie Middle Road, Guangzhou City 100.
Detailed description of the invention
Embodiment 1: this example demonstrates that the culture medium that the present invention uses
Isolation medium: Rhizoma Solani tuber osi 200g/L, glucose 20g/L, agar powder 20g/L, mercuric chloride (0-900mg/L).
Fermentation medium: Rhizoma Solani tuber osi 200g/L, glucose 20g/L.
Embodiment 2: the screening of bacterial strain F13, separate and identify
Weigh that to carry out gradient after 1 g is dissolved in 100 ml physiological saline solution from the soil sample in heavy metal pollution district, Xinjiang dilute
Release, choose 10-2、10-3、10-4Diluent is respectively coated on the isolation medium flat board containing heavy metal Hg, 3 weights of each gradient
Multiple, put 28 DEG C of cultivations.The bacterium colony that picking shape, size, color etc. are different after growing bacterium colony streak inoculation respectively is in accordingly
Flat board, until falling without miscellaneous bacteria.The F13 bacterial strain determined after cultivating screening in 28 DEG C of cultivation 6-7d, observation form, bacterium colony initial stage is
White, thickness, yeast sample；Later stage transfers brownish black to, protruding, produces without obvious polysaccharide.Basis of microscopic observation has filamentous.Reference
" Fungal identification handbook " carries out system Physiology and biochemistry qualification to F13 bacterial strain, determines that F13 bacterial strain is through Physiology and biochemistry detectionAureobasidiumBelong to bacterium.
Obtained the LSU rDNA sequence of F13 bacterial strain by PCR, divide with evolving through sequencing and BLAST homology alignment
Analysis, result show F13 bacterial strain withAureobasidium pullulans var. subglaciale EXF-2480LSU rDNA
Homology be 100%, therefore by F13 Strain Designation beAureobasidium pullulans F13。
Embodiment 3: bacterial strainAureobasidium pullulans The mutation of F13
Take one, the aureobasidium pullulans F13 inclined-plane covering with spore, with normal saline eluting spore, make spore suspension, dilute
The concentration released to spore is 106Individual/ml, takes the above-mentioned spore suspension of 2ml and adds 1ml 0.1mol/L sodium nitrite, 28 DEG C of water
Bath 5min, adds 2ml acetate buffer solution (pH 4.5), adds 2ml 0.7mol/L Na after water-bath certain time2HPO4, terminate anti-
Should.
By the spore after mutation, carrying out serial dilutions, dilution factor is respectively 10-1、10-2.Take 200ul to coat and contain
Have in the screening culture medium of metal mercury ions of variable concentrations, cultivate 6-7 days for 28 DEG C.Period, observe the bacterium colony shape on flat board
State, and add up total plate count, the bacterium colony that picking tolerance hydrargyrum concentration is the highest, finally select bacterial strain F134M.
By named for bacterial strain F134MAureobasidium pullulans F134M, was preserved in Guangdong Province before the applying date
Culture Collection, address: Xianlie Middle Road, Guangzhou City 100, preservation day it is JIUYUE in 2016 26, preserving number is
GDMCC No: 60070。
The resistance to heavy metal performance of embodiment 4:F134M bacterial strain
Original strain F13 and F134M bacterial strain are respectively coated in the sieve containing hydrargyrum 400,500,600,700,800,900 mg/L
Select on culture medium flat plate, 28 DEG C of cultivations.F13 is normal growth on the flat board reaching 500 mg/L containing hydrargyrum concentration；F134M bacterial strain
Normal growth on the flat board reaching 700 mg/L containing hydrargyrum concentration, its to hydrargyrum toleration is original strain 1.4 times.
The absorption property of embodiment 5:F134M heavy metal
Being inoculated in respectively in fermentation medium by F134M bacterial strain, under the conditions of rotating speed 150-200rpm, 6-7d is cultivated in concussion, is sent out
Ferment liquid.Fermentation liquid is centrifuged, removes culture medium, it is thus achieved that thalline.Take 1 g thalline be placed in contain 50 respectively, 100,150,200,
250, the mercury content in the 1L heavy metal Hg contaminant water of 300 mg/L, in detection contaminant water.When hydrargyrum concentration is less than 200 mg/L,
F134M to the adsorption rate of hydrargyrum more than 99%；When hydrargyrum concentration is 250 mg/ml, and F134M is 79.2% to the adsorption rate of hydrargyrum；When hydrargyrum is dense
Degree is 300 mg/ml, and F134M is 63.9% to the adsorption rate of hydrargyrum.
The computational methods of adsorption rate (R):
R(%)=(C0-Ct)/C0*100%
Wherein, C0For the initial concentration of metal ion in solution, CtAdding thalline absorption t hour is the metal ion in solution
Concentration.
Embodiment 6: the investigation of bacterial strain F134M stability
F134M bacterial strain is carried out Secondary Culture, preservation respectively.Take pass on the F134M bacterial strain of 1,10,20,50 times be respectively coated in
On screening culture medium flat board containing hydrargyrum 700 mg/L, 28 DEG C of cultivations.The F134M bacterial strain being passaged to 50 times all can be dense containing hydrargyrum
Degree reaches normal growth on the flat board of 700 mg/L.
Take respectively and pass on the strain of 1,10,20,50 times and be inoculated in respectively in fermentation medium, rotating speed 150-200rpm condition
6-7d is cultivated in lower concussion, obtains fermentation liquid.Fermentation liquid is centrifuged, removes culture medium, it is thus achieved that thalline.Take 1 g thalline be placed in containing
Mercury content in the 1L heavy metal Hg contaminant water of 200 mg/L, in detection contaminant water.It is passaged to the F134M bacterial strain of 50 times to hydrargyrum
Adsorption rate all can exceed that 99%.
Claims (4)
1. a strain Aureobasidium pullulans bacteria strain, it is characterised in that its classification is entitledAureobasidium pullulans
F134M, is preserved in Guangdong Province's Culture Collection, and deposit number is: GDMCC NO:60070.
2. bacterial strain as claimed in claim 1, it is characterised in that the adsorbable heavy metal Hg of this bacterial strain.
3. bacterial strain as claimed in claim 1, it is characterised in that this bacterial strain is 700 mg/L to the highest tolerable concentration of hydrargyrum.
4. bacterial strain application in heavy metal Hg pollution control as claimed in claim 1.
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| CN112264459A (en) * | 2020-09-30 | 2021-01-26 | 齐齐哈尔大学 | Application of aureobasidium pullulans JB16 in repairing heavy metal contaminated soil |
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| CN110551639A (en) * | 2019-09-09 | 2019-12-10 | 南京工业大学 | aureobasidium pullulans strain and application thereof in synthesis of 2, 5-dihydroxymethylfuran |
| CN112264459A (en) * | 2020-09-30 | 2021-01-26 | 齐齐哈尔大学 | Application of aureobasidium pullulans JB16 in repairing heavy metal contaminated soil |
| CN115322908A (en) * | 2022-06-01 | 2022-11-11 | 自然资源部第三海洋研究所 | Salt-tolerant aureobasidium pullulans and application thereof in water body dephosphorization |
| CN115322908B (en) * | 2022-06-01 | 2024-01-05 | 自然资源部第三海洋研究所 | Salt-tolerant aureobasidium pullulans and application thereof in dephosphorization of water body |
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