CN106236756B - A kind of chlorophyll ointment and preparation method thereof that mosquito control is bitten - Google Patents
A kind of chlorophyll ointment and preparation method thereof that mosquito control is bitten Download PDFInfo
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Abstract
The invention belongs to field of medicaments, and in particular to a kind of chlorophyll ointment and preparation method thereof that mosquito control is bitten.The chlorophyll ointment that mosquito control provided by the invention is bitten mainly is made of chlorophyll, Nuciferine, syringic acid, oil phase substrate, emulsifier, penetration enhancer, preservative and water.The chlorophyll ointment that mosquito control provided by the invention is bitten has the effect of significant anti-inflammatory anti-itch, mosquito repellent；And the chlorophyll ointment percutaneous absorbtion rate that the mosquito control is bitten is high, drugloading rate and skin permeation rate are high, the wound that bite by mosquitos can quickly and effectively be treated is a kind of ointment that ideal mosquito control is bitten, is conducive to the promotion and application for the chlorophyll ointment that the mosquito control is bitten.
Description
Technical field
The invention belongs to field of medicaments, and in particular to a kind of chlorophyll ointment and preparation method thereof that mosquito control is bitten.
Background technique
Chlorophyll is the green pigment in photosynthetic membrane, it is the main component that light is captured in photosynthesis.Chlorophyll
It is the nitrogen on magnesium and four pyrrole rings in conjunction with using porphyrin as the general name of the green pigment of skeleton, it is several shares a, b, c, d and e etc..
In recent years, green food receives people's extensive concern, and some physiological functions for the chlorophyll being present in green plants
Start to have attracted much attention.
The structure and ferroheme of chlorophyll are extremely similar, are easily absorbed by the body, and play the role of assigning living cells, in medicine, food
There is extensive use in product and daily chemical industry, since chlorophyll is natural product, has no toxic side effect, long-term consumption is safe and reliable, and
Chlorophyll also has improvement constipation, reduces cholesterol, anti-aging, toxin expelling anti-inflammatory, deodorization, the anti-mutation of anticancer, anti-anemia action and liver protection
Etc. functions, have palingenesis to skin histology, can be used for treating scald, chronic ulcer can inhibit staphylococcus and streptococcus raw
It is long, there is certain curative effect to gingivitis, halitosis, tympanitis etc., there is biggish medical value.
Chinese patent CN103417401B discloses a kind of chlorophyll toothpaste, the chlorophyll toothpaste have heat-clearing, anti-inflammatory,
Dispel halitosis, except bacterial plaque, antiallergy, cleaning tooth, the protection multi-efficiencies such as gum, can total care oral cavity, protect oral cavity and tooth
Tooth health.A kind of chlorophyll hair growth liquor of Chinese patent application 201310582887.5, the chlorophyll hair growth liquor is for alopecia areata, general
Bald, whole alopecia symptom has regeneration and anti-off effect, especially for alopecia seborrheica and heredity are early, mid-term is dilute, alopecia has obviously
Effect.Chinese patent application 201410426768.5 discloses a kind of clean face cream of chlorophyll, and the clean face cream foam of chlorophyll is thin
It is greasy, mild non-stimulated, it enables skin salubrious delicate, mellow and full not tight, pale flexible after face cleaning, is a kind of ideal clean face
Cream.
The mosquito in summer brings very big harm, and drone sound hovers in one's ear, disturbs one's sleep, and mosquito
The diseases such as malaria, filariasis, Japanese Type-B encephalitis and dengue fever can also be propagated.In face of the attack of mosquito, people are except progress ring
Border regulation and physical isolation are outer, and hygienic insecticide and culicifuge are the important channels of the mosquito of prevention and treatment.
Currently, Chinese patent application 201510655157.2 discloses a kind of lotion that mosquito control is bitten and its preparation side
Method, the lotion contain the following substances: Sweet Almond Oil, safflower oil, rose corn oil, Purple Perilla Seed Oil, citronella oil, Radix zanthoxyli mention
Liquid, Asian puccoon is taken to impregnate oil, peppermint extracting solution, xanthan gum, native fibroin, D-lactic acid sodium, ethylparaben, 75% ethyl alcohol
Solution, compound emulsifying agent AC-402,1,2- propylene glycol, APG type emulsifier SMONTANOV L, distilled water.The lotion being prepared
Property is mild, highly-safe, can supplement nutrition, the moisture of skin needs, prevent skin water shortage, achievees the effect that nourish and cure,
Skin blood can be accelerated to recycle, boosted metabolism, repair bite by mosquitos bring skin problem in time, it is effectively antipruritic.
Chinese patent application 201510723104.X discloses a kind of ointment and preparation method thereof that mosquito control is bitten, institute
Stating ointment includes that following parts by weight prepare raw material: folium artemisiae argyi, the root of Dahurain angelica, purslane, lotus leaf, glycerol, atoleine, stearic acid, three
Ethanol amine and water.The mild muting sensitive of the ointment that the mosquito control being prepared is bitten, Quick itch stopping detumescence, can prevent and treat mosquito
It bites.
However, the ointment absorptivity that current mosquito control bites paste is lower, need to use for a long time, therapeutic effect
It is unobvious.Therefore, it is high to research and develop out a kind of transdermal absorption factor, treats mosquito wound significant effect and mosquito repellent time is lasting
The ointment that mosquito control is bitten is the problem of current urgent need to resolve.
Summary of the invention
In order to overcome defect existing for the external preparation that mosquito control is bitten in the prior art, it is an object of the invention to mention
For a kind of chlorophyll ointment and preparation method thereof that mosquito control is bitten, to solve the above problem.
The present invention provides a kind of chlorophyll ointment that mosquito control is bitten, including following components and its weight percent:
Chlorophyll 10-20%, Nuciferine 8-12%, syringic acid 4-8%, oil phase substrate 12-24%, emulsifier 6-10%,
Penetration enhancer 2-6%, preservative 0.1-0.2% and water 30-45%.
Further, the chlorophyll ointment that the mosquito control is bitten includes following components and its weight percent:
Chlorophyll 16%, Nuciferine 10%, syringic acid 6%, oil phase substrate 20%, emulsifier 8%, penetration enhancer 4%,
Preservative 0.12% and water 35.9%.
Further, the oil phase substrate is by beeswax, lecithin and isopropyl myristate by weight 1-3:4-6:
0.5-2 composition.
Further, the oil phase substrate is made of beeswax, lecithin and isopropyl myristate by weight 2:5:1.
Further, the emulsifier is made of tristerin and triethanolamine by weight 2-4:1-3.
Further, the emulsifier is made of tristerin and triethanolamine by weight 3:2.
Further, the penetration enhancer is made of urea and menthol by weight 2-4:3-5.
Further, the penetration enhancer is made of urea and menthol by weight 3:4.
Further, the preservative is ethyl-para-hydroxybenzoate.
In addition, the present invention also provides a kind of preparation method of chlorophyll ointment that mosquito control is bitten, including following step
It is rapid:
S1 takes oil phase substrate to be heated to 75-85 DEG C of dissolution, is subsequently added into Nuciferine and syringic acid, stirring to being completely dissolved,
Obtain oil phase liquid；
Emulsifier is added to the water by S2 is heated to 75-85 DEG C, stirs to being completely dissolved, obtains water phase liquid；
The oil phase liquid that step S1 is obtained is added in the water phase liquid that step S2 is obtained and mixes by S3, is cooled to 30-40 DEG C, is added
Chlorophyll, penetration enhancer and preservative are 6-8 in pH value, and revolving speed stirs 30-60min under conditions of being 1200-1500rpm,
To obtain the final product.
The chlorophyll ointment Determination of Chlorophyll that mosquito control provided by the invention is bitten is purchased from the limited public affairs of Haining Feng Ming chlorophyll
Department, article No. 65963-40-8.Entitled 3, the 5- dimethoxy-4 '-hydroxybenzoic acid of chemistry of syringic acid, No. CAS is 530-57-
4；The molecular formula of Nuciferine is C19H21NO2, No. CAS is 475-83-2.The molecular formula of lecithin is C42H84O9PN, No. CAS is
8002-43-5；Beeswax is purchased from Xi'an Zao Lutang rehabilitation Pharmaceuticals Ltd, and No. CAS of tristerin is 123-94-4, meat
No. CAS of isopropyl myristate is 110-27-0.
The chlorophyll ointment that mosquito control provided by the invention is bitten has significant anti-inflammatory anti-itch effect.It is sent out through test
Existing, the ear method of double differences of the swollen mouse of caused by dimethylbenzene xylene can be significantly reduced in the chlorophyll ointment that mosquito control provided by the invention is bitten,
Anti-inflammatory effects are better than Xiao Yan Zhi Tong Oint, meanwhile, use the cavy for the chlorophyll ointment that mosquito control provided by the invention is bitten
Antipruritic valve be greater than 83.6, antipruritic effect is better than the therapeutic effect of Xiao Yan Zhi Tong Oint, illustrates prevention and treatment mosquito provided by the invention
Mutually coordinated anti-inflammatory anti-itch effect of chlorophyll ointment each component of sting.
Further, it is found through experiment that, the chlorophyll ointment that mosquito control provided by the invention is bitten has significant drive
Mosquito effect, the mosquito repellent time that is averaged are 6 hours, and the chlorophyll ointment bitten of the mosquito control is to skin safe, non-stimulated,
It will not cause allergic reaction, many people is suitble to use.
The chlorophyll ointment transdermal absorption factor with higher that mosquito control provided by the invention is bitten.It is found through experiment that
The percutaneous absorption rate for the chlorophyll ointment that mosquito control provided by the invention is bitten is greater than 4.10 μ g/cm2, illustrate the present invention by
Beeswax, lecithin and isopropyl myristate press the oil phase substrate of certain weight ratio composition, by tristerin and three ethyl alcohol
Amine presses the emulsifier of certain weight ratio composition, can be effective by the penetration enhancer that certain weight ratio forms by urea and menthol
The skin permeation rate for improving ointment, is more advantageous to the healing of bite by mosquitos wound.
In short, the chlorophyll ointment that mosquito control provided by the invention is bitten, has the advantage that compared with prior art
1) the chlorophyll Ointment that mosquito control provided by the invention is bitten is clear, and toxic side effect is small, drugloading rate
Height has the effect of significant anti-inflammatory anti-itch, mosquito repellent；
2) the chlorophyll ointment percutaneous absorbtion rate that mosquito control provided by the invention is bitten is high, is a kind of ideal prevention and treatment mosquito
The chlorophyll ointment of sting is conducive to the promotion and application for the chlorophyll ointment that the mosquito control is bitten.
Specific embodiment
The present invention is further described below by way of specific embodiment, the present invention is not limited only to following embodiment.In this hair
In bright range or the contents of the present invention are not being departed from, in spirit and scope, the change that carries out to the present invention is combined or replaced
It changes, will be apparent to the person skilled in the art, and be included within the scope of the present invention.
Embodiment 1, a kind of chlorophyll ointment that mosquito control is bitten
The chlorophyll ointment that the mosquito control is bitten is made of following components and its weight percent:
Chlorophyll 10%, Nuciferine 8%, syringic acid 4%, oil phase substrate 24%, emulsifier 10%, penetration enhancer 6%,
Ethyl-para-hydroxybenzoate 0.1% and water 37.9%；The oil phase substrate is by beeswax, lecithin and isopropyl myristate by weight
Amount is formed than 1:6:0.5；The emulsifier is made of tristerin and triethanolamine by weight 2:3；The infiltration promotees
It is made of into agent urea and menthol by weight 2:5.
Preparation method:
S1 takes oil phase substrate to be heated to 75 DEG C of dissolutions, is subsequently added into Nuciferine and syringic acid, stirs to being completely dissolved, obtains oily
Phase liquid；
Emulsifier is added to the water by S2 is heated to 75 DEG C, stirs to being completely dissolved, obtains water phase liquid；
The oil phase liquid that step S1 is obtained is added in the water phase liquid that step S2 is obtained and mixes by S3, is cooled to 30 DEG C, and leaf is added
Green element, penetration enhancer and preservative are 6 in pH value, under conditions of revolving speed is 1200rpm stirring 60min to get.
Embodiment 2, a kind of chlorophyll ointment that mosquito control is bitten
The chlorophyll ointment that the mosquito control is bitten is made of following components and its weight percent:
Chlorophyll 16%, Nuciferine 10%, syringic acid 6%, oil phase substrate 20%, emulsifier 8%, penetration enhancer 4%,
Ethyl-para-hydroxybenzoate 0.12% and water 35.9%；The oil phase substrate is pressed by beeswax, lecithin and isopropyl myristate
Weight ratio 2:5:1 composition；The emulsifier is made of tristerin and triethanolamine by weight 3:2；The infiltration promotees
It is made of into agent urea and menthol by weight 3:4.
Preparation method:
S1 takes oil phase substrate to be heated to 80 DEG C of dissolutions, is subsequently added into Nuciferine and syringic acid, stirs to being completely dissolved, obtains oily
Phase liquid；
Emulsifier is added to the water by S2 is heated to 80 DEG C, stirs to being completely dissolved, obtains water phase liquid；
The oil phase liquid that step S1 is obtained is added in the water phase liquid that step S2 is obtained and mixes by S3, is cooled to 35 DEG C, and leaf is added
Green element, penetration enhancer and preservative are 7 in pH value, under conditions of revolving speed is 1400rpm stirring 40min to get.
Embodiment 3, a kind of chlorophyll ointment that mosquito control is bitten
The chlorophyll ointment that the mosquito control is bitten is made of following components and its weight percent:
Chlorophyll 20%, Nuciferine 12%, syringic acid 8%, oil phase substrate 12%, emulsifier 6%, penetration enhancer 2%,
Ethyl-para-hydroxybenzoate 0.16% and water 39.84%；The oil phase substrate is pressed by beeswax, lecithin and isopropyl myristate
Weight ratio 3:4:2 composition；The emulsifier is made of tristerin and triethanolamine by weight 4:1；The infiltration promotees
It is made of into agent urea and menthol by weight 4:3.
Preparation method:
S1 takes oil phase substrate to be heated to 85 DEG C of dissolutions, is subsequently added into Nuciferine and syringic acid, stirs to being completely dissolved, obtains oily
Phase liquid；
Emulsifier is added to the water by S2 is heated to 85 DEG C, stirs to being completely dissolved, obtains water phase liquid；
The oil phase liquid that step S1 is obtained is added in the water phase liquid that step S2 is obtained and mixes by S3, is cooled to 40 DEG C, and leaf is added
Green element, penetration enhancer and preservative are 8 in pH value, under conditions of revolving speed is 1500rpm stirring 30min to get.
Comparative example 1, a kind of chlorophyll ointment that mosquito control is bitten
The chlorophyll ointment that the mosquito control is bitten is made of following components and its weight percent:
Chlorophyll 22%, Nuciferine 10%, oil phase substrate 20%, emulsifier 8%, penetration enhancer 4%, para hydroxybenzene first
Acetoacetic ester 0.12% and water 35.9%；The oil phase substrate is by beeswax, lecithin and isopropyl myristate by weight 2:5:1
Composition；The emulsifier is made of tristerin and triethanolamine by weight 3:2；The penetration enhancer by urea and
Menthol is formed by weight 3:4.
Preparation method is similar to Example 2.
The difference from example 2 is that not adding syringic acid, increase the parts by weight of chlorophyll.
Comparative example 2, a kind of chlorophyll ointment that mosquito control is bitten
The chlorophyll ointment that the mosquito control is bitten includes following components and its weight percent:
Chlorophyll 16%, Nuciferine 10%, gallic acid 6%, oil phase substrate 20%, emulsifier 8%, penetration enhancer
4%, ethyl-para-hydroxybenzoate 0.12% and water 35.9%；The oil phase substrate is by beeswax, lecithin and myristic acid isopropyl
Ester is formed by weight 2:5:1；The emulsifier is made of tristerin and triethanolamine by weight 3:2；The infiltration
Saturating promotor is made of urea and menthol by weight 3:4.
Preparation method is similar to Example 2.
The difference from example 2 is that syringic acid is replaced with gallic acid.
Comparative example 3, a kind of chlorophyll ointment that mosquito control is bitten
The chlorophyll ointment that the mosquito control is bitten is made of following components and its weight percent:
Chlorophyll 16%, Nuciferine 10%, syringic acid 6%, oil phase substrate 20%, emulsifier 8%, penetration enhancer 4%,
Ethyl-para-hydroxybenzoate 0.12% and water 35.9%；The oil phase substrate is by beeswax and isopropyl myristate by weight 1:
1 composition；The emulsifier is made of tristerin and triethanolamine by weight 3:2；The penetration enhancer is by urea
It is formed with menthol by weight 3:4.
Preparation method is similar to Example 2.
The difference from example 2 is that the oil phase substrate is by beeswax and isopropyl myristate by weight 1:1 group
At.Comparative example 4, a kind of chlorophyll ointment that mosquito control is bitten
The chlorophyll ointment that the mosquito control is bitten includes following components and its weight percent:
Chlorophyll 16%, Nuciferine 10%, syringic acid 6%, oil phase substrate 20%, emulsifier 8%, penetration enhancer 4%,
Ethyl-para-hydroxybenzoate 0.12% and water 35.9%；The oil phase substrate is pressed by beeswax, lecithin and isopropyl myristate
Weight ratio 1:1:1 composition；The emulsifier is made of tristerin and triethanolamine by weight 3:2；The infiltration promotees
It is made of into agent urea and menthol by weight 3:4.
Preparation method is similar to Example 2.
The difference from example 2 is that the oil phase substrate by beeswax, lecithin and isopropyl myristate by weight
1:1:1 composition.
Comparative example 5, a kind of chlorophyll ointment that mosquito control is bitten
The chlorophyll ointment that the mosquito control is bitten includes following components and its weight percent:
Chlorophyll 16%, Nuciferine 10%, syringic acid 6%, oil phase substrate 20%, emulsifier 8%, penetration enhancer 4%,
Ethyl-para-hydroxybenzoate 0.12% and water 35.9%；The oil phase substrate is pressed by beeswax, lecithin and isopropyl myristate
Weight ratio 2:5:1 composition；The emulsifier is made of tristerin and triethanolamine by weight 3:2；The infiltration promotees
It is made of into agent urea and menthol by weight 3:4.
Preparation method is similar to Example 2.
The difference from example 2 is that the emulsifier is by tristerin and triethanolamine by weight 1:1 group
At.Comparative example 6, a kind of chlorophyll ointment that mosquito control is bitten
The chlorophyll ointment that the mosquito control is bitten is made of following components and its weight percent:
Chlorophyll 16%, Nuciferine 10%, syringic acid 6%, oil phase substrate 20%, emulsifier 8%, penetration enhancer 4%,
Ethyl-para-hydroxybenzoate 0.12% and water 35.9%；The oil phase substrate is pressed by beeswax, lecithin and isopropyl myristate
Weight ratio 2:5:1 composition；The emulsifier is made of tristerin and triethanolamine by weight 3:2；The infiltration promotees
It is made of into agent urea and menthol by weight 3:4.
Preparation method is similar to Example 2.
The difference from example 2 is that the penetration enhancer is made of urea and menthol by weight 1:1.
The anti-inflammatory for the chlorophyll ointment that test example one, mosquito control are bitten is tested
1,60 Kunming mouses, half male and half female, weight 25-30g, by Yunnan University of Traditional Chinese Medicine's reality subjects: are chosen
Center offer is provided.
2, test material: it is soft that embodiment 1, embodiment 2, comparative example 1 and comparative example 2 prepare the chlorophyll that mosquito control is bitten
Cream；Xiao Yan Zhi Tong Oint is purchased from Guangdong Hengjian Pharmaceutical Co., Ltd., national drug standard Z44023482.
3, test method:
60 mouse are randomly divided into 6 groups, respectively model group, Xiao Yan Zhi Tong Oint group, example 1 group, embodiment 2
Group, 1 group of comparative example and 2 groups of comparative example, then uniformly apply 50 μ l of dimethylbenzene in each group mouse right ear, left ear is opposed by every group 10
According to, cause it is scorching after after 1h, coating, each group dosage is as follows:
Model group: the physiological saline of equivalent is taken to be applied to the affected part of mouse ear；
Xiao Yan Zhi Tong Oint group: the Xiao Yan Zhi Tong Oint of 1g/kg is taken to be applied to the affected part of mouse ear；
Example 1 group: the chlorophyll ointment that the mosquito control for taking the embodiment 1 of 1g/kg to prepare is bitten is applied to mouse ear
The affected part in portion；
2 groups of embodiment: the chlorophyll ointment that the mosquito control for taking the embodiment 2 of 1g/kg to prepare is bitten is applied to mouse ear
The affected part in portion；
1 group of comparative example: the chlorophyll ointment that the mosquito control for taking the comparative example 1 of 1g/kg to prepare is bitten is applied to mouse ear
The affected part in portion；
2 groups of comparative example: the chlorophyll ointment that the mosquito control for taking the comparative example 2 of 1g/kg to prepare is bitten is applied to mouse ear
The affected part in portion；
Above-mentioned ointment is applied respectively 3 times, interval 30min is applied 1 time.30min takes off vertebra and puts to death after last coating, cuts two along auricle
Ear lays round auricle at the same position of left and right ear respectively with 8mm punch, weighing, calculates the ear method of double differences, the ear method of double differences=auris dextra circle
Auricle weight-left ear circle auricle weight.
4, test result:
Test result is as shown in table 1.
The influence that the chlorophyll ointment that 1 mosquito control of table is bitten swells to mouse auricular concha caused by dimethylbenzene xylene
| Group | The ear method of double differences (mg) |
| Control group | 8.12±1.30 |
| Xiao Yan Zhi Tong Oint group | 5.70±0.42** |
| Example 1 group | 4.83±0.36** |
| 2 groups of embodiment | 4.08±0.35** |
| 1 group of comparative example | 7.28±0.98 |
| 2 groups of comparative example | 7.85±1.04 |
Note: compared with model group group**P < 0.01.
As shown in Table 1, compared with model group, example 1 group and 2 groups of embodiment can be extremely significant reduce the ear weight of mouse
Difference, anti-inflammatory effects are better than Xiao Yan Zhi Tong Oint, and 1 group of comparative example and 2 groups of comparative example of the ear method of double differences are compared with the control group without system
Meter learns meaning, and the chlorophyll ointment compatibility for illustrating that mosquito control provided by the invention is bitten is reasonable, and can cooperate with enhances its anti-inflammatory
Effect.
The antipruritic test for the chlorophyll ointment that test example two, mosquito control are bitten
1,60 cavys, weight 250-300g, half male and half female, by Zhongshan University's Experimental Animal Center subjects: are chosen
It provides.
2, test material: it is soft that embodiment 1, embodiment 2, comparative example 1 and comparative example 2 prepare the chlorophyll that mosquito control is bitten
Cream；Xiao Yan Zhi Tong Oint is purchased from Guangdong Hengjian Pharmaceutical Co., Ltd., national drug standard Z44023482.
3, test method:
60 cavys are randomly divided into 6 groups, every group 10, before test for 24 hours, to instep shaving behind each group cavy right side, press this
Group dosage is instep shaving coating 1 time after animal is right.The same day is tested, is abraded at right metapedes shaving with coarse sandpaper, area 1cm2, office
Portion's coating 1 time again starts after 10min at surface of a wound Chu Di 0.01% histamine phosphate 0.05ml/, and each group dose is as follows:
Model group: the physiological saline of equivalent is taken to be applied to the affected part of mouse dorsal portion；
Xiao Yan Zhi Tong Oint group: the Xiao Yan Zhi Tong Oint of 2g/kg is taken to be applied to the affected part of mouse dorsal portion；
Example 1 group: the chlorophyll ointment that the mosquito control for taking the embodiment 1 of 0.1g/kg to prepare is bitten is applied to mouse
The affected part of dorsal portion；
2 groups of embodiment: the chlorophyll ointment that the mosquito control for taking the embodiment 2 of 0.1g/kg to prepare is bitten is applied to mouse
The affected part of dorsal portion；
1 group of comparative example: the chlorophyll ointment that the mosquito control for taking the comparative example 1 of 0.1g/kg to prepare is bitten is applied to mouse
The affected part of dorsal portion；
2 groups of comparative example: the chlorophyll ointment that the mosquito control for taking the comparative example 2 of 0.1g/kg to prepare is bitten is applied to mouse
The affected part of dorsal portion；
Hereafter 0.02%, 0.03%, 0.04% enrichment degree coating is successively used every 3min, is every time 0.05ml/.Directly
Histamine phosphate's total amount (μ g) to be administered is itch-threshold when later licking right metapedes to cavy, records and the cause for comparing each group is itched
Threshold.
4, test result
Test result is as shown in table 2.
The antipruritic test for the chlorophyll ointment that 2 mosquito control of table is bitten
| Group | Antipruritic valve (histamine phosphate total amount μ g) |
| Model group | 32.4±11.5 |
| Xiao Yan Zhi Tong Oint group | 72.5±11.56** |
| Example 1 group | 83.6±30.43** |
| 2 groups of embodiment | 90.6±30.14** |
| 1 group of comparative example | 48.8±10.32 |
| 2 groups of comparative example | 40.6±13.65 |
Note: compared with model group group**P < 0.01.
As shown in Table 2, the chlorophyll that 2 groups of mosquito controls being prepared of 1 group of the embodiment of the present invention and embodiment are bitten is soft
The antipruritic valve of cream is greater than 83.6, and antipruritic effect is better than the therapeutic effect of Xiao Yan Zhi Tong Oint, and 1 group of comparative example and comparative example 2
The antipruritic effect for the chlorophyll ointment that the mosquito control that group is prepared is bitten is not statistically significant compared with model group, illustrates this
Mutually coordinated antipruritic effect of chlorophyll ointment each component that the mosquito control that invention provides is bitten.
The safety detection for the chlorophyll ointment that test example three, mosquito control are bitten
Prevent according to the requirement of " new drug toxicologic study guideline " made from embodiment 1, embodiment 2 and embodiment 3
The chlorophyll ointment for controlling bite by mosquitos carries out skin irritation, anaphylaxis evaluation, the results are shown in Table 3.
The antiphlogistic and analgesic ointment safety detection result of the present invention of table 3
| Group | Phenomenon | Conclusion |
| Embodiment 1 | Without erythema, without oedema | It is non-stimulated, without sensitization |
| Embodiment 2 | Without erythema, without oedema | It is non-stimulated, without sensitization |
| Embodiment 3 | Without erythema, without oedema | It is non-stimulated, without sensitization |
As shown in Table 3, the chlorophyll ointment that mosquito control provided by the invention is bitten, will not to skin safe, non-stimulated
It causes allergic reaction, broad masses is suitble to use.
The repelling effect for the chlorophyll ointment that test example four, mosquito control are bitten is tested:
1, object is chosen: laggard at laboratory rearing emergence 2-3 days using the field-collected strains Culex quinquefasciatus worm of collection in worksite
Row measurement.
2, test material: embodiment 2 prepares the chlorophyll ointment that mosquito control is bitten.
3, test method: randomly selecting 200 mosquitoes, and being put into length, width and height is to choose 8 tests in the mosquito cage of 40cm
Person, wherein male 4, select a hand to smear the embodiment of the present invention 2 and prepare the chlorophyll ointment that mosquito control is bitten by women 4,
As test group；Another hand is as a control group.After smearing two hours, puts his hand into mosquito cage 2 minutes, seen whether mosquito
It sucks blood, the measurement of per half an hour is primary later, until stopping observation when the hand for being coated with essential oil has bite by mosquitos, terminate test,
Record effective protecting time.
4, test result:
The effective protecting time of 8 experimenters is respectively as follows: 6,7,6,5,5.5,7,6.5,5 hours, is computed and learns, this hair
The mosquito repellent for the chlorophyll ointment that the mosquito control of bright offer is bitten, average effective guard time can reach 6 hours, illustrate the present invention
The chlorophyll ointment repelling effect that the mosquito control of offer is bitten is significant.
Test example five, skin permeation test in vitro
1, subjects: embodiment 1, embodiment 2, embodiment 3, comparative example 3, comparative example 4, comparative example 5 and comparative example 6 are made
The chlorophyll ointment that standby mosquito control is bitten.
2, test method: using the Franz two-chamber osmotic device of improvement, with isolated mouse skin for transdermal barrier, with leaf
Green element is index, using external diffusion cell method, carries out vitro permeation assay.3g embodiment 1, embodiment 2, embodiment are weighed respectively
3, bite 3 parts of chlorophyll ointment of mosquito control prepared by comparative example 3, comparative example 4, comparative example 5 and comparative example 6, are uniformly smeared
Penetrating absorption is carried out after isolated mouse skin, respectively after administration 1,2,4,6,8,10,12,14h respectively take acceptable solution 1mL, adopt
With the accumulation infiltration capacity of high effective liquid chromatography for measuring chlorophyll, 1ml is supplemented into reception tank containing 2% Tween 80 after sampling
PBS liquid.Its calculation formula for accumulating infiltration capacity is as follows:
Wherein: Cn is the drug concentration that n-th of sample point measures；A is infiltrating area；Ci is i-th (i < n -1) a sampling
The drug concentration that point measures.To accumulate the average value of infiltration capacity respectively to time t respectively by zero level, level-one and Higuchi equation
It is fitted, the slope of gained equation is percutaneous rate constant J.Wherein diffusion pool area is 2.92cm2, receiving liquid volume is
7ml, receiving liquid temperature are 37 ± 0.5 DEG C, magnon revolving speed 200rmin-1, receiving liquid is the PBS liquid containing 2% Tween-80.
3, test result:
Test result is as shown in table 4, and with zero-order transdermal transfer, then the slope of Q-t straight line is percutaneous absorption rate.
The skin permeation test in vitro for the chlorophyll ointment that 4 mosquito control of table is bitten
| Group | Percutaneous absorption rate (μ g/cm2) |
| Embodiment 1 | 4.10 |
| Embodiment 2 | 4.30 |
| Embodiment 3 | 4.12 |
| Comparative example 3 | 3.88 |
| Comparative example 4 | 3.98 |
| Comparative example 5 | 2.87 |
| Comparative example 6 | 3.35 |
As shown in Table 4, the Transdermal absorption for the chlorophyll ointment that the mosquito control that 1-3 of the embodiment of the present invention is prepared is bitten
Rate is greater than 4.10 μ g/cm2, and the Transdermal absorption for the chlorophyll ointment that the mosquito control that comparative example 2-6 is prepared is bitten is fast
Percutaneous absorption rate of the rate than the cream of embodiment of the present invention 1-3 is low, illustrates the present invention by beeswax, lecithin and nutmeg
Isopropyl propionate presses the oil phase substrate of certain weight ratio composition, is made of tristerin and triethanolamine by certain weight ratio
Emulsifier can effectively improve the skin permeation rate of ointment by urea and menthol, more by the penetration enhancer that certain weight ratio forms
Be conducive to the healing of bite by mosquitos wound.
Claims (9)
1. a kind of chlorophyll ointment that mosquito control is bitten, which is characterized in that including following components and its weight percent:
Chlorophyll 10-20%, Nuciferine 8-12%, syringic acid 4-8%, oil phase substrate 12-24%, emulsifier 6-10%, penetration enhancer
2-6%, preservative 0.1-0.2% and water 30-45%.
2. the chlorophyll ointment that mosquito control as described in claim 1 is bitten, which is characterized in that the oil phase substrate is by bee
Wax, lecithin and isopropyl myristate are formed by weight 1-3:4-6:0.5-2.
3. the chlorophyll ointment that mosquito control as claimed in claim 2 is bitten, which is characterized in that the oil phase substrate is by bee
Wax, lecithin and isopropyl myristate are formed by weight 2:5:1.
4. the chlorophyll ointment that mosquito control as described in claim 1 is bitten, which is characterized in that the emulsifier is by stearic acid
Glyceride and triethanolamine are formed by weight 2-4:1-3.
5. the chlorophyll ointment that mosquito control as claimed in claim 4 is bitten, which is characterized in that the emulsifier is by stearic acid
Glyceride and triethanolamine are formed by weight 3:2.
6. the chlorophyll ointment that mosquito control as described in claim 1 is bitten, which is characterized in that the penetration enhancer is by urinating
Element and menthol are formed by weight 2-4:3-5.
7. the chlorophyll ointment that mosquito control as claimed in claim 6 is bitten, which is characterized in that the penetration enhancer is by urinating
Element and menthol are formed by weight 3:4.
8. the chlorophyll ointment that mosquito control as described in claim 1 is bitten, which is characterized in that the preservative is to hydroxyl
Ethyl benzoate.
9. a kind of preparation method for the chlorophyll ointment that mosquito control a method as claimed in any one of claims 1-8 is bitten, feature exist
In, comprising the following steps:
S1 takes oil phase substrate to be heated to 75-85 DEG C of dissolution, is subsequently added into Nuciferine and syringic acid, stirs to being completely dissolved, obtains oily
Phase liquid；
Emulsifier is added to the water by S2 is heated to 75-85 DEG C, stirs to being completely dissolved, obtains water phase liquid；
The oil phase liquid that step S1 is obtained is added in the water phase liquid that step S2 is obtained and mixes by S3, is cooled to 30-40 DEG C, and it is green that leaf is added
Element, penetration enhancer and preservative are 6-8 in pH value, and revolving speed stirs 30-60min under conditions of being 1200-1500rpm, i.e.,
?.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102846485A (en) * | 2012-08-31 | 2013-01-02 | 广州市科玮生物技术有限公司 | Chlorophyll sun-screening repair cosmetic |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102846485A (en) * | 2012-08-31 | 2013-01-02 | 广州市科玮生物技术有限公司 | Chlorophyll sun-screening repair cosmetic |
Non-Patent Citations (2)
| Title |
|---|
| Chlorophyll derivatives can be an efficient weapon in the fight against dengue;Azizullah Azizullah等;《Parasitology Research》;20141015;第113卷;第4321-4326页 |
| 荷叶碱的药理作用研究进展;戴谆等;《中国药师》;20160630;第19卷(第5期);第988-991页 |
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