CN106226536A - A kind of position phase variation test method of Salmonella H antigen - Google Patents
A kind of position phase variation test method of Salmonella H antigen Download PDFInfo
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Abstract
The invention discloses the position phase variation test method of a kind of Salmonella H antigen, it carries out the agglutination reaction of serum of O antigen to Salmonella strains to be checked, the situation of hiving off according to O antigen is examined in the 1st phase and the 2nd phase H factor, when only detecting a phase and bacterial strain to be checked is not single-phase bacterium, it is defined as aimed strain, afterwards known phase H factor serum and aimed strain are added to sterile vegetative meat soup, after cultivation, centrifugal collection bacterial sediment, obtains bacteria suspension for treating the slide agglutination inspection of phase-detecting H antigen after washing bacterium.After we's tagmeme phase variation, the slide agglutination speed of H antigen is fast, phenomenon substantially, disturb little, reproducible, success rate is high, easy and simple to handle, there is higher feasibility and practicality.
Description
Technical field
The present invention relates to the position phase variation test method of a kind of Salmonella H antigen.
Background technology
Salmonella is zoonosis pathogen significant on public hygienics, its correct serotype
In infectious disease control significant.Detect Salmonella serogroup 2500 kinds at present, existing more than 290 serotypes of China
Report.The Serotype Identification of Salmonella is more complicated, and its serotype is together decided on by O antigen, the 1st phase and the 2nd phase H antigen etc.
(some only has a phase H factor), and the mensuration of H antigen is the Main Basis of Salmonella serotype.H antigen is positioned at flagellum
On, it is in the nature the proteantigen of instability, has stronger specificity, heat, be dried, the factor such as freezing or Ethanol Treatment
Make flagella structural impaired, cause H antigenicity to weaken, thus appraisal is brought difficulty.Such as, experimenter is had to find in freezer
The salmonella strain of the sample separation that the resting period is the longest, reaches the passage number needed for H antigenicity is restored the most.Daily qualification work
Work is frequently encountered by the flagellum dysplasia of Salmonella and the indefinite situation of typing, in standard GB/T 4789.4-2010
In recommend the traditional position phase variation method of simple flat band method, glass-tube method and voltage regulator tube method 3 kinds to induce another phase H factor.Other
Scientific worker also discloses that the modification method that some phase variation are tested, such as nutrient broth-Nutrient agar method, coubling dilution
Deng.Wherein simple flat band method is widely used because it is simple and convenient, but its distinct disadvantage to be success rate low；Glass-tube method and voltage regulator tube method
Material be there are certain requirements, such as capillary tube and little voltage regulator tube, and operation complexity.In addition said method uses Nutrient agar or semisolid
Soft agar is as the substrate of strain growth, and the mobility of the existence suppression culture medium of agar, this exists with the kinetic characteristic of flagellum
Antagonism, thus hinder the antigenic recovery of H.Additionally, Salmonella can break through low concentration agar, diffuse to inside culture medium
Growth, causes thalline picking difficulty, and therefore when carrying out the slide agglutination test of H antigen, culture medium is inevitably with thalline
Entering in serum drop, and cannot be completely dispersed, small solid medium granule is easily obscured with serum agglutination granule, thus
The judgement of severe jamming coagulation result.It addition, the agglutinator of H antigen is cotton-shaped, it is difficult to observe, experiment generally requires picking relatively
Many thalline are to strengthen coagulation effect, and this is conflicting with the purpose reducing solid medium interference.In view of the foregoing, at present
Position phase variation test method have that interference is big, success rate is low, operate the shortcoming such as many complicated, time-consuming, it is impossible to meet increasing
Inspection work demand.
Summary of the invention
For disadvantages mentioned above, success rate is high, reproducible, it is low to disturb, operation letter to it is an object of the invention to provide one
The position phase variation test method of Salmonella H antigen the most efficiently, for reaching above-mentioned purpose, the technical solution used in the present invention
It is:
The position phase variation test method of a kind of Salmonella H antigen, comprises the following steps:
A. Salmonella strains to be checked is carried out the agglutination reaction of serum of O antigen, is examined according to the situation of hiving off of O antigen
1st phase and the 2nd phase H factor, when only detecting a phase and bacterial strain to be checked is not single-phase bacterium, be defined as aimed strain；
B. take known phase H factor serum in sterile vegetative meat soup (serum is 1:200 ~ 1 with the ratio of the volume of nutrient broth:
800), mixing, vaccination target bacterial strain, cultivate 18 h ~ 24 h in 36 DEG C ± 1 DEG C, it is thus achieved that culture；
C. being transferred in sterile centrifugation tube by culture, under the conditions of 5000 ~ 8000r/min, centrifugal 5 ~ 10min, moves and abandons supernatant,
Retain the bacterial sediment bottom centrifuge tube；
D. in the centrifuge tube containing bacterial sediment, add the physiological saline solution (ratio of physiological saline solution and the volume of culture
For 1:1 ~ 1:3), with pipettor pressure-vaccum gently, until thalline suspends completely, under the conditions of 5000 ~ 8000 r/min, again it is centrifuged 5
~ 10 min, move and abandon supernatant, complete to wash bacterium step；Wash bacterium step can be repeated 1 times or repeatedly；
E. in the centrifuge tube completing to wash bacterium step, add the physiological saline solution (ratio of bacterial sediment and the volume of sterile physiological salt
For 1:2 ~ 1:5), mix with pipettor pressure-vaccum gently, obtain bacteria suspension；
F. take 10 ~ 15 μ L and treat that phase-detecting H factor serum drips on clean slide, take 1 ~ 2 μ L bacteria suspension and add to serum drop
In, mix and tilt to shake slide, within 1 min, observing agglutination phenomenon；Simultaneously at another region physiological saline solution of slide
Replace treating phase-detecting H factor serum, as compareing after mixing with bacteria suspension.
Compared with prior art, the invention has the beneficial effects as follows: use the nutrient broth of liquid to replace the cultivation containing agar
Base as the substrate of strain growth, eliminates agar to the interference of slide agglutination and the antagonism of flagellum growth promoter.Liquid
The composition of culture medium is uniform, and the nutriment that microorganism can be fully contacted and utilize in culture medium, flagellum is as the motion of Salmonella
Organ, can fully play motion effect and significantly be induced growth in liquid medium within, more conducively H is antigenic extensive
Multiple.During agglutination test, higher H antigenicity makes agglutination phenomenon become apparent from, and does not has the interference of solid medium granule, and coagulation is tied
Fruit is easier to observe and judge.
The present invention collects bacterial sediment by centrifugal, it is easier to obtain more thalline to realize strengthening the mesh of coagulation effect
, being suspended in bacteria suspension of thalline relative distribution used during agglutination test, it is easier to diffusion uniformly, makes coagulation in serum
Speed faster, phenomenon become apparent from, disturb less.Additionally, the experimental condition that specify that each step quantitative in method and each component
Dosage, make the repeatability of phase variation test more preferably, have higher success rate.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage become apparent from, below in conjunction with embodiment, the present invention is carried out
Further describe in detail, it will be appreciated that described embodiment is only in order to explain the present invention.Every skill based on the present invention
Simplification that art scheme is made or equivalent variations, belong to protection scope of the present invention.
Embodiment 1:
(Salmonella inspection examined by the 2015 blind samples of state food pharmaceuticals administration food safety sampling observation detection Cheng Jian mechanism of general bureau
Test), totally 10 parts of samples, numbered CODE1 ~ 10, if detection Salmonella need to carry out serotype to isolated strains.Through inspection
Surveying, in 10 parts of samples, CODE1, CODE4, CODE5, CODE6 totally 4 parts of samples are that Salmonella is positive.Enter the most according to the following steps
Row follow-up test:
A. 4 Salmonella strains to be checked are carried out the agglutination reaction of serum of O antigen, according to the situation of hiving off of O antigen successively
Checking the 1st phase and the 2nd phase H factor, qualification result is CODE1(O 7:H r), CODE4(O 4:H i), CODE5(O 4:H f,
G, s), CODE6(O 19:H i), wherein the bacterial strain to be checked of CODE5 is single-phase bacterium, so determining that CODE1, CODE4, CODE6 are common
The Salmonella positive strain of 3 samples is aimed strain.
B. known phase H factor serum is taken to (serum is 1:200 with the ratio of the volume of nutrient broth) in sterile vegetative meat soup,
Mixing, vaccination target bacterial strain, cultivate 18 h in 36 DEG C ± 1 DEG C, it is thus achieved that culture；
C. culture is transferred in sterile centrifugation tube, centrifugal 10 min under the conditions of 5000 r/min, moves and abandon supernatant, retain from
Bacterial sediment bottom heart pipe；
D. in the centrifuge tube containing bacterial sediment, add the physiological saline solution (ratio of physiological saline solution and the volume of culture
For 1:1), with pipettor pressure-vaccum gently, until thalline suspends completely, centrifugal 10 min under the conditions of 5000 r/min, move again
Abandon supernatant, complete to wash bacterium step；
E. in the centrifuge tube completing to wash bacterium step, add the physiological saline solution (ratio of bacterial sediment and the volume of sterile physiological salt
For 1:2), mix with pipettor pressure-vaccum gently, obtain bacteria suspension；
F. take 10 μ L and treat that phase-detecting H factor serum drips on clean slide, take 1 μ L bacteria suspension and add to serum drop, mixed
Even and tilt to shake slide, observe agglutination phenomenon within 1 min；Replace treating at another region physiological saline solution of slide simultaneously
Phase-detecting H factor serum, as compareing after mixing with bacteria suspension；H antigen position phase variation result of the test is CODE1(H 7), CODE4
(H 2), CODE6(H z6).
The complete qualification result of serotype is as shown in table 1, simultaneously with simple flat band method qualification result carried out repeatedly flat
Row checking, confirmatory experiment shows that the qualification result of two kinds of methods is identical, and completely the same with blind sample examination measurement result.
Embodiment 2:
This grade of food safety sampling observation of state food pharmaceuticals administration office of general bureau in 2016 detects Cheng Jian mechanism blind sample assessment mode,
Wherein Salmonella inspection totally 10 parts of samples, numbered CODE1 ~ 10, report detects or does not detects Salmonella.10 parts after testing
In sample, CODE2, CODE7, CODE8 totally 3 samples are that Salmonella is positive.Carry out follow-up test the most according to the following steps:
A. 3 Salmonella strains to be checked are carried out the agglutination reaction of serum of O antigen, according to the situation of hiving off of O antigen successively
Check the 1st phase and the 2nd phase H factor, check that result is CODE2(O 7:H c)；CODE7(O 4:H f, g, s), CODE8(O 7:H
C), wherein the bacterial strain to be checked of CODE7 is single-phase bacterium, so determining the Salmonella positive strain of CODE2, CODE8 totally 2 samples
For aimed strain.
B. known phase H factor serum is taken to (serum is 1:500 with the ratio of the volume of nutrient broth) in sterile vegetative meat soup,
Mixing, vaccination target bacterial strain, cultivate 20 h in 36 DEG C ± 1 DEG C, it is thus achieved that culture；
C. culture is transferred in sterile centrifugation tube, centrifugal 5 min under the conditions of 6000 r/min, moves and abandon supernatant, retain from
Bacterial sediment bottom heart pipe；
D. in the centrifuge tube containing bacterial sediment, add the physiological saline solution (ratio of physiological saline solution and the volume of culture
For 1:2), with pipettor pressure-vaccum gently, until thalline suspends completely, centrifugal 5 min under the conditions of 6000 r/min again, shifting is abandoned
Supernatant, completes to wash bacterium step；
E. in the centrifuge tube completing to wash bacterium step, add the physiological saline solution (ratio of bacterial sediment and the volume of sterile physiological salt
For 1:3), mix with pipettor pressure-vaccum gently, obtain bacteria suspension；
F. take 13 μ L and treat that phase-detecting H factor serum drips on clean slide, take 1 μ L bacteria suspension and add to serum drop, mixed
Even and tilt to shake slide, observe agglutination phenomenon within 1 min；Replace treating at another region physiological saline solution of slide simultaneously
Phase-detecting H factor serum, as compareing after mixing with bacteria suspension；H antigen position phase variation result of the test is CODE2(H 5), CODE8
(H 5).
The complete qualification result of serotype is as shown in table 2, carries out parallel by simple flat band method to serotype result equally
Checking, the result shows that the qualification result of two kinds of methods is completely the same.
Embodiment 3:
One strain Salmonella typhimurium (strain number: CGMCC1.1174) is carried out the confirmatory experiment of serotype.Will bacterial strain
Follow-up test is carried out according to the following steps after activation:
A. Salmonella strains to be checked is carried out the agglutination reaction of serum of O antigen, is examined according to the situation of hiving off of O antigen
1st phase and the 2nd phase H factor, qualification result is O 4:H i, so being defined as aimed strain;
B. known phase H factor serum is taken to (serum is 1:800 with the ratio of the volume of nutrient broth) in sterile vegetative meat soup, mixed
Even, vaccination target bacterial strain, cultivate 24 h in 36 DEG C ± 1 DEG C, it is thus achieved that culture；
C. culture is transferred in sterile centrifugation tube, centrifugal 5 min under the conditions of 8000 r/min, moves and abandon supernatant, retain from
Bacterial sediment bottom heart pipe；
D. in the centrifuge tube containing bacterial sediment, add the physiological saline solution (ratio of physiological saline solution and the volume of culture
For 1:3), with pipettor pressure-vaccum gently, until thalline suspends completely, centrifugal 5 min under the conditions of 8000 r/min again, shifting is abandoned
Supernatant, completes to wash bacterium step；Wash bacterium step and repeat 1 time；
E. in the centrifuge tube completing to wash bacterium step, add the physiological saline solution (ratio of bacterial sediment and the volume of sterile physiological salt
For 1:5), mix with pipettor pressure-vaccum gently, obtain bacteria suspension；
F. take 15 μ L and treat that phase-detecting H factor serum drips on clean slide, take 2 μ L bacteria suspensions and add to serum drop, mixed
Even and tilt to shake slide, observe agglutination phenomenon within 1 min；Replace treating at another region physiological saline solution of slide simultaneously
Phase-detecting H factor serum, as compareing after mixing with bacteria suspension；
The slide agglutination of the 2nd phase H antigen of aimed strain checks that result is H 2, and the serotype meeting Salmonella typhimurium is special
Levy.
In the implementation process of above-mentioned 3 embodiments, when finding to use technical scheme H after position phase variation because of
Sub-serum agglutination speed faster, phenomenon become apparent from, disturb little, the repeatability of position phase variation test more preferably, have higher success rate.Reliably
Qualification result also illustrate that the position phase variation test method of Salmonella H antigen of the present invention has higher feasibility and practicality
Property.
It is familiar with one of ordinary skill in the art it should be appreciated that the experimental condition of technical solution of the present invention includes certain
Scope, such as: during training objective bacterial strain, serum is 1:200 ~ 1:800 with the ratio of the volume of nutrient broth, the cultivation of aimed strain
Time is 18 ~ 24 h, when culture is centrifuged centrifugal rotational speed be 5000 ~ 8000 r/min, centrifugation time be 5 ~ 10 min, wash bacterium
In step, physiological saline solution is 1:1 ~ 1:3 with the ratio of the volume of culture, washes bacterium step and can be repeated 1 times or repeatedly, make
During standby bacteria suspension, bacterial sediment is 1:2 ~ 1:5 with the ratio of the volume of sterile physiological salt, and on clean slide, the phase-detecting H factor is treated in dropping
During serum, the dripping quantity of serum is 10 ~ 15 μ L, and when adding bacteria suspension in serum drop, the addition of bacteria suspension is 1 ~ 2 μ L.
When using the experimental condition in above-mentioned scope, technical scheme also can reach embodiment 1, embodiment 2 or embodiment 3
Identical effect.
Above by description of listed embodiment, elaborate the basic ideas and basic principles of the present invention.Obviously, institute
The embodiment stated is only a part of embodiment of the present invention, and indefiniteness is exhaustive.Based on the embodiment in the present invention, ability
All other embodiments that territory those of ordinary skill is obtained on the premise of not paying creative work, broadly fall into the present invention
Protection domain.
Claims (1)
1. a position phase variation test method for Salmonella H antigen, comprises the following steps:
A. Salmonella strains to be checked is carried out the agglutination reaction of serum of O antigen, is examined according to the situation of hiving off of O antigen
1st phase and the 2nd phase H factor, when only detecting a phase and bacterial strain to be checked is not single-phase bacterium, be defined as aimed strain；
B. taking known phase H factor serum in sterile vegetative meat soup, serum is 1:200 ~ 1 with the ratio of the volume of nutrient broth:
800, mixing, vaccination target bacterial strain, cultivate 18 h ~ 24 h in 36 DEG C ± 1 DEG C, it is thus achieved that culture；
C. being transferred in sterile centrifugation tube by culture, under the conditions of 5000 ~ 8000 r/min, centrifugal 5 ~ 10 min, move and abandon
Clearly, the bacterial sediment bottom centrifuge tube is retained；
D adds physiological saline solution in the centrifuge tube containing bacterial sediment, the ratio of physiological saline solution and the volume of culture
For 1:1 ~ 1:3, with pipettor pressure-vaccum gently, until thalline suspends completely, again centrifugal 5 under the conditions of 5000 ~ 8000 r/min ~
10 min, move and abandon supernatant, complete to wash bacterium step；Wash bacterium step can be repeated 1 times or repeatedly；
E adds physiological saline solution in the centrifuge tube completing to wash bacterium step, the ratio of bacterial sediment and the volume of sterile physiological salt
For 1:2 ~ 1:5, mix with pipettor pressure-vaccum gently, obtain bacteria suspension；
F. take 10 ~ 15 μ L and treat that phase-detecting H factor serum drips on clean slide, take 1 ~ 2 μ L bacteria suspension and add to serum drop
In, mix and tilt to shake slide, within 1 min, observing agglutination phenomenon；Simultaneously at another region physiological saline solution of slide
Replace treating phase-detecting H factor serum, as compareing after mixing with bacteria suspension.
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993020231A1 (en) * | 1992-03-31 | 1993-10-14 | The Minister Of Agriculture, Fisheries And Food In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Method of testing for salmonella |
| RU2063769C1 (en) * | 1992-11-11 | 1996-07-20 | Государственный научно-исследовательский институт биосинтеза белковых веществ | Method of preparing specific salmonellosis polyvalent h-serum for accelerated salmonella detection in foodstuffs, fodder, environment object and clinical material |
| WO2001019960A1 (en) * | 1999-09-10 | 2001-03-22 | Women's And Children's Hospital | Recombinant microorganisms expressing an oligosaccharide receptor mimic |
| CN1570637A (en) * | 2003-07-25 | 2005-01-26 | 大连康基食品安全检测技术有限公司 | Fast detection device for salmonella |
| JP2010035552A (en) * | 2008-07-08 | 2010-02-18 | National Agriculture & Food Research Organization | Method for quickly identifying salmonella typhimurium, choleraesuis, enteritidis, dublin and gallinarum by multiply detecting specific gene |
| CN101955993A (en) * | 2010-06-13 | 2011-01-26 | 中华人民共和国珠海出入境检验检疫局 | Compound gene chip for detecting salmonella serotype and detection method thereof |
| CN102031282A (en) * | 2010-11-19 | 2011-04-27 | 上海市疾病预防控制中心 | Kit of ready-to-use salmonella identification plate, preparation method and use method |
| WO2012058303A2 (en) * | 2010-10-26 | 2012-05-03 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention | Rapid salmonella serotyping assay |
| CN103614453A (en) * | 2013-05-16 | 2014-03-05 | 黑龙江中医药大学 | Gastrointestinal bacteria detection method for medical microbiology experiment |
| CN103725780A (en) * | 2013-12-18 | 2014-04-16 | 广东省微生物研究所 | Method for detecting and identifying salmonella 1, 4, [5], 12:i- |
| CN104059977A (en) * | 2014-06-25 | 2014-09-24 | 上海交通大学 | Salmonella serotype identification method and kit thereof |
| CN104087654A (en) * | 2013-04-01 | 2014-10-08 | 中国农业大学 | Multiple PCR identification kit of salmonella and five serotypes of salmonella |
-
2016
- 2016-08-01 CN CN201610618448.9A patent/CN106226536B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993020231A1 (en) * | 1992-03-31 | 1993-10-14 | The Minister Of Agriculture, Fisheries And Food In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Method of testing for salmonella |
| RU2063769C1 (en) * | 1992-11-11 | 1996-07-20 | Государственный научно-исследовательский институт биосинтеза белковых веществ | Method of preparing specific salmonellosis polyvalent h-serum for accelerated salmonella detection in foodstuffs, fodder, environment object and clinical material |
| WO2001019960A1 (en) * | 1999-09-10 | 2001-03-22 | Women's And Children's Hospital | Recombinant microorganisms expressing an oligosaccharide receptor mimic |
| CN1570637A (en) * | 2003-07-25 | 2005-01-26 | 大连康基食品安全检测技术有限公司 | Fast detection device for salmonella |
| JP2010035552A (en) * | 2008-07-08 | 2010-02-18 | National Agriculture & Food Research Organization | Method for quickly identifying salmonella typhimurium, choleraesuis, enteritidis, dublin and gallinarum by multiply detecting specific gene |
| CN101955993A (en) * | 2010-06-13 | 2011-01-26 | 中华人民共和国珠海出入境检验检疫局 | Compound gene chip for detecting salmonella serotype and detection method thereof |
| WO2012058303A2 (en) * | 2010-10-26 | 2012-05-03 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention | Rapid salmonella serotyping assay |
| CN102031282A (en) * | 2010-11-19 | 2011-04-27 | 上海市疾病预防控制中心 | Kit of ready-to-use salmonella identification plate, preparation method and use method |
| CN104087654A (en) * | 2013-04-01 | 2014-10-08 | 中国农业大学 | Multiple PCR identification kit of salmonella and five serotypes of salmonella |
| CN103614453A (en) * | 2013-05-16 | 2014-03-05 | 黑龙江中医药大学 | Gastrointestinal bacteria detection method for medical microbiology experiment |
| CN103725780A (en) * | 2013-12-18 | 2014-04-16 | 广东省微生物研究所 | Method for detecting and identifying salmonella 1, 4, [5], 12:i- |
| CN104059977A (en) * | 2014-06-25 | 2014-09-24 | 上海交通大学 | Salmonella serotype identification method and kit thereof |
Non-Patent Citations (4)
| Title |
|---|
| LISA BARCO,ET AL: "A Rapid and Sensitive Method to Identify and Differentiate Salmonella enterica Serotype Typhimurium and Salmonella enterica Serotype 4,[5],12:i:- by Combining Traditional Serotyping and Multiplex Polymerase Chain Reaction", 《FOODBORNE PATHOGENS AND DISEASE》 * |
| MARY V. JESUDASON,ET AL: "Vi-Specific Latex Agglutination for Early and Rapid Detection of Salmonella Serotype typhi in Blood Cultures", 《DIAGN MICROBIOL INFECT DIS》 * |
| 侯水平 等: "1种沙门菌位相变异实验的方法学研究", 《中国卫生检验杂志》 * |
| 邱亚群 等: "M肉汤法在沙门菌血清分型中的应用", 《热带医学杂志》 * |
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| CN106226536B (en) | 2017-11-10 |
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