CN106214731A - Blueberry raw anthocyanidin biological extraction process - Google Patents
Blueberry raw anthocyanidin biological extraction process Download PDFInfo
- Publication number
- CN106214731A CN106214731A CN201610762644.3A CN201610762644A CN106214731A CN 106214731 A CN106214731 A CN 106214731A CN 201610762644 A CN201610762644 A CN 201610762644A CN 106214731 A CN106214731 A CN 106214731A
- Authority
- CN
- China
- Prior art keywords
- filtrate
- extraction process
- biological extraction
- blueberry raw
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/45—Ericaceae or Vacciniaceae (Heath or Blueberry family), e.g. blueberry, cranberry or bilberry
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to technical field of biological extraction, be specifically related to a kind of blueberry raw anthocyanidin biological extraction process.Blueberry raw anthocyanidin biological extraction process, step is: mixed with blue berry berry marc powder by acidic ethanol, heating and refluxing extraction, filter and collect filtrate and residue, residue mixes with the acidic ethanol solution of identical solid-liquid ratio with same concentration again, reflux, extract, again, filters and collects filtrate, merges the filtrate of twice；Filtrate low temperature is placed, then filters, concentration is evaporated under reduced pressure；Then pass through microporous filter membrane and carry out hyperfiltration treatment, to remove antibacterial and high molecular weight protein；Finally by column chromatography purification, drying and other steps.The present invention proposes the biological extraction process of a kind of blueberry raw anthocyanidin, and the method, according to different product categories, can extract the procyanidin that purity is 25～51%, and this technology is current domestic state-of-the-art blueberry raw anthocyanidin extractive technique.And, compared with external similar technique, also there is lower-cost advantage.
Description
Technical field
The present invention relates to technical field of biological extraction, be specifically related to a kind of blueberry raw anthocyanidin biological extraction process.
Background technology
Containing abundant procyanidin nutritional labeling in Blueberry, procyanidin not only has good nourishing healthy and makees
With, also have and prevent that cranial nerve is aging, heart tonifying, anticancer vessel softening, strengthen the functions such as human organism's immunity.Effect of procyanidin
Value expression exists:
(1) antioxidation and removing free radical: OPC are owing to there being the hydroxylic moiety of electron rich in its molecular structure, are excellent
Hydrogen or the donor of neutron, therefore have superpower oxidation resistance, can effectively remove the free radical in human body.Procyanidin exists
Vivo and vitro has antioxidant activity.
(2) protection cardio-cerebrovascular: procyanidin is obvious to the protective effect of cerebral ischemia reperfusion injury；People can be suppressed
The propagation of class skin capillary endothelial cell, affects angiogenesis；Having clear and definite antiplatelet aggregative activity, antithrombotic is made
With substantially；Reduce plasma triglyceride free fatty apolipoprotein B, improve Atherosclerosis Risk index after the meal；Have
Stronger adjustment blood fat, promotes reverse cholesterol transport and accelerates lipid and Cholesterol Excretion, improving the effect of lipid metabolic disorder.
(3) anti-curing cancers: procyanidin has antitumor action, to cervical cancer, many cancers such as carcinoma of prostate, colon cancer
There are prevention and the effect for the treatment of.Additionally, the cancers such as skin carcinoma, oral cancer, hepatocarcinoma are still had certain effect by procyanidin.
(4) prevent with treatment diabetes: procyanidin can improve the oxidised form of diabetic disease states, to insulin sensitivity
The ILA of cell line and antioxidant activity.
(5) antiinflammatory action: procyanidin as antiinflammatory, thus plays antiinflammatory in the macrophage that endotoxin stimulates
Effect.
(6) gastric ulcer injury protection effect: procyanidin can suppress the generation of stress gastric ulcer, suppression gastric mucosa
Secretion acid function, reduces pepsic activity simultaneously.
(7) the liver protecting effect: low dose of (50,100mg L-1) procyanidin can suppress ethanol and tetrachloro-methane induction
Hepatocyte injury and stimulate normal liver cell propagation.
(8) radiation resistance: procyanidin can reduce the Fas protein expression integration of roentgenization skin, increases Bc1-2
Protein expression integration, is allowed to close to normal skin.
(9) enzyme inhibition: procyanidin has inhibitory action to multiple enzyme, it can effectively suppress external pancreatic lipase
Activity, its effect with the degree of polymerization increase and strengthen.
(10) other effects: procyanidin also has mutation, antiallergic, anti HIV-1 virus, antidepressant, antifatigue effect,
Treatment acute respiratory distress syndrome, delays the decline of audition, promotes hair growth and analgesic activity.
At present, blueberry raw anthocyanidin carrying out separation and Extraction and deep processing is always industrial hot spot, external Producer is existing
Utilizing the research that blueberry raw anthocyanidin pharmacy, fine chemical product research and development etc. are relevant, this measure can be substantially improved value of the product and profit
Profit return rate, widens the development space of blueberry industry simultaneously.
Summary of the invention
For technical problem present in prior art, it is an object of the invention to provide a kind of blueberry raw anthocyanidin biological
Extracting method, to achieve these goals, the technical solution adopted in the present invention is:
A kind of blueberry raw anthocyanidin biological extraction process, step is as follows:
1) extract: by concentration be 35～75%, pH value be ethanol and the blue berry berry marc powder of 2.5～5.5 with 3～9:
1 part by weight mixing, heating and refluxing extraction 1～2h, filter and collect filtrate and residue, residue again with identical solid-liquid ratio with
Mix with the acidic ethanol solution of concentration, reflux, extract, 1～2h again, filter and collect filtrate, merge the filtrate of twice；
2) remove impurity: by step 1) gained merging filtrate 0～4 DEG C of low temperature put 15～20h, then filter；
3) concentrate: by step 2) gained filtrate is evaporated under reduced pressure at 55 DEG C concentrate 10～12h；
4) ultrafiltration: by step 3) gained concentrated solution surpassed by microporous filter membrane (aperture may select 0.40um and 0.20um)
Filter processes, to remove antibacterial and high molecular weight protein；
5) column chromatography purification: first filtrate is flow through macroporous adsorbent resin, then with the speed of per hour 1～3 times of column volume
Flow through resin with the distilled water of 2～5 times of column volumes with identical speed, wash away major part impurity, then with the 60 of 1～3 column volumes
～the ethanol solution of 90% flows through resin with identical speed, the anthocyanin that resin adsorbs is eluted, collect eluent；
6) it is dried: eluent is evaporated under reduced pressure at 55 DEG C concentration and removes ethanol further, finally in 40～55 DEG C of vacuum
Environment is dried.
The blueberry raw anthocyanidin biological extraction process of the present invention, its beneficial effect shows:
The present invention proposes the biological extraction process of a kind of blueberry raw anthocyanidin, the method according to different product categories,
Can extract the procyanidin that purity is 25～51%, this technology is that current domestic state-of-the-art blueberry raw anthocyanidin extracts skill
Art.And, compared with external similar technique, also there is lower-cost advantage.
Accompanying drawing explanation
Fig. 1 is the flow chart of the blueberry raw anthocyanidin biological extraction process of the present invention.
Detailed description of the invention
For further describing the blueberry raw anthocyanidin biological extraction process of the present invention, below in conjunction with the accompanying drawings it is made further
Explanation.
Embodiment 1
Referring to Fig. 1, a kind of blueberry raw anthocyanidin biological extraction process, step is as follows:
1) extract: by concentration be 60%, pH value be that ethanol and the blue berry berry marc powder of 4.5 are with the part by weight of 5:1
Mixing, heating and refluxing extraction 1.5h, filter and collect filtrate and residue, residue is again with identical solid-liquid ratio and the acidifying with concentration
Ethanol solution mixes, and reflux, extract, 1.5h again filters and collects filtrate, merges the filtrate of twice.
2) remove impurity: by step 1) 2 DEG C of low temperature of gained merging filtrate put 18h, then filter.
3) concentrate: by step 2) gained filtrate is evaporated under reduced pressure at 55 DEG C concentrate 11h.
4) ultrafiltration: by step 3) gained concentrated solution carries out hyperfiltration treatment by microporous filter membrane, to remove antibacterial and macromole
Albumen.
5) column chromatography purification: first filtrate is flow through macroporous adsorbent resin with the speed of per hour 2 times of column volumes, then with 3
The distilled water of times column volume flows through resin with identical speed, washes away major part impurity, then the ethanol with the 70% of 1 column volume is molten
Liquid flows through resin with identical speed, is eluted by the anthocyanin that resin adsorbs, and collects eluent.
6) it is dried: eluent is evaporated under reduced pressure at 55 DEG C concentration and removes ethanol further, finally 50 DEG C of vacuum environments
In be dried, obtain blueberry raw anthocyanidin, in extract, anthocyanin content is 48%.
Embodiment 2
A kind of blueberry raw anthocyanidin biological extraction process, step is as follows:
1) extract: by concentration be 70%, pH value be 3 ethanol mix with the part by weight of 8:1 with blue berry berry marc powder
Closing, heating and refluxing extraction 1.2h, filter and collect filtrate and residue, residue is again with identical solid-liquid ratio and the acidifying second with concentration
Alcoholic solution mixes, and reflux, extract, 1.2h again filters and collects filtrate, merges the filtrate of twice.
2) remove impurity: by step 1) 3 DEG C of low temperature of gained merging filtrate put 16h, then filter.
3) concentrate: by step 2) gained filtrate is evaporated under reduced pressure at 55 DEG C concentrate 12h.
4) ultrafiltration: by step 3) gained concentrated solution carries out hyperfiltration treatment by microporous filter membrane, to remove antibacterial and macromole
Albumen.
5) column chromatography purification: first filtrate is flow through macroporous adsorbent resin with the speed of per hour 1 times of column volume, then with 5
The distilled water of times column volume flows through resin with identical speed, washes away major part impurity, then the ethanol with the 80% of 1 column volume is molten
Liquid flows through resin with identical speed, is eluted by the anthocyanin that resin adsorbs, and collects eluent.
6) it is dried: eluent is evaporated under reduced pressure at 55 DEG C concentration and removes ethanol further, finally 45 DEG C of vacuum environments
In be dried, obtain blueberry raw anthocyanidin, in extract, anthocyanin content is 35%.
Embodiment 3
A kind of blueberry raw anthocyanidin biological extraction process, step is as follows:
1) extract: by concentration be 40%, pH value be 5 ethanol mix with the part by weight of 4:1 with blue berry berry marc powder
Closing, heating and refluxing extraction 2h, filter and collect filtrate and residue, residue is again with identical solid-liquid ratio and the acidic ethanol with concentration
Solution mixes, and reflux, extract, 2h again filters and collects filtrate, merges the filtrate of twice.
2) remove impurity: by step 1) 1 DEG C of low temperature of gained merging filtrate puts 19h, then filters.
3) concentrate: by step 2) gained filtrate is evaporated under reduced pressure at 55 DEG C concentrate 10h.
4) ultrafiltration: by step 3) gained concentrated solution carries out hyperfiltration treatment by microporous filter membrane, to remove antibacterial and macromole
Albumen.
5) column chromatography purification: first filtrate is flow through macroporous adsorbent resin with the speed of per hour 3 times of column volumes, then with 2
The distilled water of times column volume flows through resin with identical speed, washes away major part impurity, then the ethanol with the 65% of 3 column volumes is molten
Liquid flows through resin with identical speed, is eluted by the anthocyanin that resin adsorbs, and collects eluent.
6) it is dried: eluent is evaporated under reduced pressure at 55 DEG C concentration and removes ethanol further, finally in 40～55 DEG C of vacuum
Being dried in environment, obtain blueberry raw anthocyanidin, in extract, anthocyanin content is 38%.
Above content is only the design example to the present invention and explanation, affiliated those skilled in the art
Described specific embodiment is made various amendment or supplements or use similar mode to substitute, without departing from invention
Design or surmount scope defined in the claims, protection scope of the present invention all should be belonged to.
Claims (1)
1. a blueberry raw anthocyanidin biological extraction process, it is characterised in that step is as follows:
1) extract: by concentration be 35～75%, pH value be that ethanol and the blue berry berry marc powder of 2.5～5.5 are with 3～9:1
Part by weight mix, heating and refluxing extraction 1～2h, filter and collect filtrate and residue, residue again with identical solid-liquid ratio with
The acidic ethanol solution mixing of concentration, reflux, extract, 1～2h again, filter and collect filtrate, merge the filtrate of twice；
2) remove impurity: by step 1) gained merging filtrate 0～4 DEG C of low temperature put 15～20h, then filter；
3) concentrate: by step 2) gained filtrate is evaporated under reduced pressure at 55 DEG C concentrate 10～12h；
4) ultrafiltration: by step 3) gained concentrated solution carries out hyperfiltration treatment by microporous filter membrane, to remove antibacterial and high molecular weight protein；
5) column chromatography purification: first filtrate is flow through macroporous adsorbent resin with the speed of per hour 1～3 times of column volume, then with 2～
The distilled water of 5 times of column volumes flows through resin with identical speed, washes away major part impurity, then with the 60～90% of 1～3 column volumes
Ethanol solution flow through resin with identical speed, the anthocyanin that resin adsorbs is eluted, collect eluent；
6) it is dried: eluent is evaporated under reduced pressure at 55 DEG C concentration and removes ethanol further, finally 40～55 DEG C of vacuum environments
In be dried.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610762644.3A CN106214731A (en) | 2016-08-29 | 2016-08-29 | Blueberry raw anthocyanidin biological extraction process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610762644.3A CN106214731A (en) | 2016-08-29 | 2016-08-29 | Blueberry raw anthocyanidin biological extraction process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106214731A true CN106214731A (en) | 2016-12-14 |
Family
ID=58071303
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610762644.3A Pending CN106214731A (en) | 2016-08-29 | 2016-08-29 | Blueberry raw anthocyanidin biological extraction process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106214731A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109096236A (en) * | 2018-08-13 | 2018-12-28 | 安徽兆龙生物科技有限公司 | Blueberry procyanidine biological extraction process |
| CN112778260A (en) * | 2020-12-31 | 2021-05-11 | 齐鲁工业大学 | Blueberry anthocyanin-rich extract and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004026325A2 (en) * | 2002-09-18 | 2004-04-01 | Interhealth Nutraceuticals Incorporated | Composition of berry extracts that prevents or inhibits angiogenesis and helicobacter pylori |
| CN1517343A (en) * | 2003-01-13 | 2004-08-04 | 大兴安岭华野生物工程有限公司 | Extraction method of huckleberry anthocyanidin |
| CN101265252A (en) * | 2008-04-28 | 2008-09-17 | 吉林大学 | Vaccinium uliginosum cyanidin and separation and purification method thereof |
| CN101525325A (en) * | 2008-03-06 | 2009-09-09 | 陕西嘉禾植物化工有限责任公司 | Method for extracting anthocyanin from cowberry |
| CN103289430A (en) * | 2013-06-27 | 2013-09-11 | 安徽省林锦记食品工业有限公司 | Method for extracting and purifying anthocyanins from blueberry pomaces |
-
2016
- 2016-08-29 CN CN201610762644.3A patent/CN106214731A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004026325A2 (en) * | 2002-09-18 | 2004-04-01 | Interhealth Nutraceuticals Incorporated | Composition of berry extracts that prevents or inhibits angiogenesis and helicobacter pylori |
| CN1517343A (en) * | 2003-01-13 | 2004-08-04 | 大兴安岭华野生物工程有限公司 | Extraction method of huckleberry anthocyanidin |
| CN101525325A (en) * | 2008-03-06 | 2009-09-09 | 陕西嘉禾植物化工有限责任公司 | Method for extracting anthocyanin from cowberry |
| CN101265252A (en) * | 2008-04-28 | 2008-09-17 | 吉林大学 | Vaccinium uliginosum cyanidin and separation and purification method thereof |
| CN103289430A (en) * | 2013-06-27 | 2013-09-11 | 安徽省林锦记食品工业有限公司 | Method for extracting and purifying anthocyanins from blueberry pomaces |
Non-Patent Citations (6)
| Title |
|---|
| 云少君著: "《植物铁蛋白结构与功能研究》", 31 December 2014, 中国农业科学技术出版社 * |
| 安晓婷等: "大孔树脂纯化蓝莓果渣多酚及其组成分析", 《食品科学》 * |
| 张亚红等: "大孔树脂吸附及超滤膜技术提纯蓝莓花色苷中试研究", 《林业科技》 * |
| 曾青兰等主编: "《生物制药工艺》", 29 February 2012, 华中科技大学出版社 * |
| 王丹丹等: "蓝莓、紫薯与葡萄籽原花青素提取及其清除自由基能力的比较", 《辽东学院学报( 自然科学版)》 * |
| 赵美丽等: "蓝莓原花青素的提取与检测方法", 《江西化工》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109096236A (en) * | 2018-08-13 | 2018-12-28 | 安徽兆龙生物科技有限公司 | Blueberry procyanidine biological extraction process |
| CN112778260A (en) * | 2020-12-31 | 2021-05-11 | 齐鲁工业大学 | Blueberry anthocyanin-rich extract and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Vuong et al. | Extraction and isolation of catechins from tea | |
| Zhou et al. | Ganodermataceae: natural products and their related pharmacological functions | |
| CN104546980B (en) | A kind of bee pollen extract and its preparation and application | |
| EP2033528A2 (en) | Lingonberry extract, the preparing method and use thereof | |
| KR101438543B1 (en) | Preparation method of oxyresveratrol, t-resveratrol, and moracin having anti-inflammatory and anti-aging function from Mulberry twig extract | |
| CN106220748B (en) | A kind of sea asparagus general flavone and polysaccharide combined extraction method | |
| CN106214731A (en) | Blueberry raw anthocyanidin biological extraction process | |
| JP6302102B2 (en) | A compound isolated from MONASCUS PURPUREUS, its preparation and use | |
| CN105753722A (en) | Method for simultaneously extracting synephrine and flavonoids from immature bitter oranges | |
| CN105902584B (en) | Moringa oleifera leaf flavone with anticomplementary and hypoglycemic activities and preparation method and application thereof | |
| KR20110052940A (en) | A process for preparing an extract fraction enriched with ginsenoside rg3 from ginseng radix | |
| CN108355115A (en) | A kind of method of continuous extraction purification ginger polyphenol | |
| CN103103227A (en) | Preparation technology for tea polyphenol oxidized polymer with alpha-glucosidase inhibition effect | |
| Cheng et al. | State-of-the-art review of theabrownins: from preparation, structural characterization to health-promoting benefits | |
| Ko et al. | Polyphenol mixtures of Euphorbia supina the inhibit invasion and metastasis of highly metastatic breast cancer MDA-MB-231 cells | |
| CN108685796B (en) | Litchi rind total phenol extract mainly containing polymer polyphenol and application thereof | |
| CN102630943A (en) | Spirulina gamma-linolenic acid extractive and preparation method thereof | |
| CN101565422A (en) | Method for extracting and refining sanggenon C and application thereof | |
| CN102219782B (en) | Method for extracting and separating viterxin and isovitexin from natural product | |
| WO2014025169A1 (en) | Method for enhancing polyphenol and lactobacillus content of apples using fermented product of psidium guajava, and mixed concentrated solution of apples using said method | |
| CN108913499A (en) | A kind of tartary buckwheat extract and preparation method thereof, application and health liquor | |
| KR0168485B1 (en) | The production of ginseng steamed red essence | |
| US20130272974A1 (en) | Hydroxytyrosol Product, Method of Making, and Uses Thereof | |
| CN105560173B (en) | A kind of high stability astragalus injection and preparation method thereof | |
| CN1687092A (en) | General glycoside extractive of xanthium and preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 234000, room 1, building 0301, C District, two Huai River Xincheng, Suzhou, Anhui Applicant after: SHENLI BLUEBERRY GROUP CO., LTD. Address before: 234000 Anhui Province dawn Yongqiao District of Suzhou City Road No. 999 Building No. 2 (Tuscany town) Applicant before: SHENLI BLUEBERRY GROUP CO., LTD. |
|
| CB02 | Change of applicant information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Xu Huali Inventor after: Zhang Xiaobin Inventor before: Xu Huali |
|
| CB03 | Change of inventor or designer information | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161214 |
|
| RJ01 | Rejection of invention patent application after publication |