CN106198363A - A kind of FCM analysis chip - Google Patents
A kind of FCM analysis chip Download PDFInfo
- Publication number
- CN106198363A CN106198363A CN201610822592.4A CN201610822592A CN106198363A CN 106198363 A CN106198363 A CN 106198363A CN 201610822592 A CN201610822592 A CN 201610822592A CN 106198363 A CN106198363 A CN 106198363A
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- CN
- China
- Prior art keywords
- diaphragm seal
- chip
- hole
- analysis chip
- pool
- Prior art date
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- Granted
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- 238000004458 analytical method Methods 0.000 title claims abstract description 17
- 239000007788 liquid Substances 0.000 claims abstract description 50
- 238000001514 detection method Methods 0.000 claims abstract description 19
- 239000002699 waste material Substances 0.000 claims abstract description 16
- 239000012530 fluid Substances 0.000 claims abstract description 12
- 238000007689 inspection Methods 0.000 claims description 5
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 210000004276 hyalin Anatomy 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 238000009738 saturating Methods 0.000 claims description 4
- 230000005484 gravity Effects 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000003792 electrolyte Substances 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000008151 electrolyte solution Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
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- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of FCM analysis chip, be compounded with strippable first protecting film, the first diaphragm seal, micro-fluid chip and the second diaphragm seal the most successively；At least four electrode hole, a well and a venthole are set on described first diaphragm seal；On described micro-fluid chip, the direction that uses according to chip is from top to bottom provided with the liquid storage tank of connection, addition pool, sense channel and waste liquid pool, and is positioned at least two detection hole at sense channel two ends；Corresponding liquid storage tank, two detection holes and the porch of waste liquid pool are distinguished in the position that arranges of described four electrode holes；Described well position correspondence addition pool is set；Described venthole position correspondence waste liquid pool is set.The present invention utilizes gravity principle to the power providing liquid to flow, and therefore can need not additional driving, and chip volume is little, low cost, the device miniaturization used, energy-saving, arranges electrode hole simultaneously and carries out sample detecting, it is ensured that the precision of testing result.
Description
Technical field
The present invention relates to a kind of FCM analysis chip.
Background technology
Streaming detection of particulates is the technology that the fine-grained particles being in liquid carries out quantitative analysis and sorting one by one, and it can
To measure particle size, shape, concentration, activity etc., have relatively broad at subjects such as hematology, immunology, molecular biology
Application.The Coulter principle that it is used in the detection refers to: when the granule suspended in the electrolytic solution passes through aperture with electrolyte,
Replace the electrolyte of same volume, cause two resistance between electrode inside and outside aperture to occur moment to become in the circuit of constant current design
Changing, produce potential pulse, size and the number of times of pulse signal are directly proportional to size and the number of granule.It is that streaming is micro-that sample focuses on
The key technology of grain detection, is all the chip using and sample liquid being realized by External Force Acting focusing in current detection, this kind of
Integrated chip detecting electrode is in chip, and volume is relatively big, takes out negative pressure and provides power, and need in use for chip during use
Adding buffer, how buffer adds, and adds the use that how much also can affect chip.
Summary of the invention
It is an object of the invention to provide one and need not external force driving, small and exquisite FCM analysis chip easily.
The technical scheme realizing the object of the invention is a kind of FCM analysis chip, is compounded with peelable the most successively
From the first protecting film, the first diaphragm seal, micro-fluid chip and the second diaphragm seal；On described first diaphragm seal, at least four is set
Electrode hole, a well and a venthole；On described micro-fluid chip, the use direction according to chip is from top to bottom arranged
There are the liquid storage tank of connection, addition pool, sense channel and waste liquid pool, and are positioned at least two detection hole at sense channel two ends；
Corresponding liquid storage tank, two detection holes and the porch of waste liquid pool are distinguished in the position that arranges of described four electrode holes；Described sample-adding
Hole position correspondence addition pool is set；Described venthole position correspondence waste liquid pool is set.
Described each electrode hole is the dash line of at least one stroke of saturating first diaphragm seal；Preset buffering in described liquid storage tank
Liquid.
Described electrode hole arranges six；Described detection hole arranges four, and sense channel two ends respectively arrange two；Described first
On diaphragm seal, corresponding four detection hole location respectively set an electrode hole.
On described first diaphragm seal, balance pipe is set between minimum electrode hole and the venthole in position.
Described venthole is non-circular hole；Or described venthole and balance pipe are non-circular hole.
The degree of depth of liquid storage tank, addition pool, sense channel and waste liquid pool on described micro-fluid chip is different.
Described first diaphragm seal is opaque coating.
Opaque second protecting film also it is compounded with bottom described second diaphragm seal.
Described first protecting film and the second diaphragm seal are hyaline membrane.
The electrode hole that on described first diaphragm seal, position is the highest is simultaneously as air inlet.
After have employed technique scheme, the present invention has positive effect: (1) present invention utilizes gravity principle to provide
The power of liquid flowing, therefore can need not additional driving, and chip volume is little, low cost, the device miniaturization used, joint
Energyization, arranges electrode hole simultaneously and carries out sample detecting, it is ensured that the precision of testing result.
(2) electrode hole of the present invention use penetrate but not hollow out draw hole, it is simple to electrode is set during use, can hinder again simultaneously
Gear liquid, it is to avoid buffer spills, such structure just can the most preset buffer, conveniently detection.
(3) balance pipe of the present invention is positioned at above venthole, supplementing as venthole, even if going out in transport or use
Produce bubble at pore, remain to make the smooth and easy circulation of liquid.
(4) prior art all uses the venthole of circle, but the venthole liquid of circle the most inside flows, conductor liquid
Body environment disturbance, the present invention arranges non-circular balance pipe and venthole, and liquid is difficult to rock, and is conducive to stablizing liquid environment.
(5) first protecting film of the present invention can form an airtight environment for chip, it is to avoid liquid is in transport and storage
Total dislocation, relative liquid surface ambient stable, there is no liquid before use to waste liquid pool, after tearing, under gravity, liquid is
Flow down；Meanwhile, in the first protecting film can also protect chip, liquid is clean.
(6) the first diaphragm seal and second protecting film of the present invention uses opaque coating, is avoided that liquid light goes bad.
(7) second protecting film of the present invention is hyaline membrane, facilitates quality inspection.
(8) degree of depth in each pond of the chip of the present invention is different, is conducive to providing in use different driving forces.
Accompanying drawing explanation
It is clearly understood to make present disclosure be easier to, below according to specific embodiment and combine accompanying drawing, right
The present invention is described in further detail, wherein
Fig. 1 is the structural representation of the present invention.
Fig. 2 is the structural representation of first diaphragm seal of the present invention.
Fig. 3 is the structural representation of the micro-fluid chip of the present invention.
Accompanying drawing is numbered:
First protecting film 1, electrode hole the 21, first electrode hole 21-1, the second electrode hole 21-2, the 3rd electrode hole 21-3,
Four electrode hole 21-4, the 5th electrode hole 21-5, the 6th electrode hole 21-6, well 22, venthole 23, balance pipe 24, first are close
Sealer 2, micro-fluid chip 3, liquid storage tank 31, addition pool 32, waste liquid pool 33, detection hole the 34, second diaphragm seal the 4, second protecting film
5。
Detailed description of the invention
(embodiment 1)
See Fig. 1, Fig. 2 and Fig. 3, a kind of FCM analysis chip of the present embodiment, it is compounded with peelable the most successively
From first protecting film the 1, first diaphragm seal 2, micro-fluid chip the 3, second diaphragm seal 4 and the second protecting film 5；First diaphragm seal 2
On 21, well 22 of six electrode holes and a venthole 23 are set；According to the use direction of chip on micro-fluid chip 3
From top to bottom it is provided with the liquid storage tank 31 of connection, addition pool 32, sense channel and waste liquid pool 33, and is positioned at sense channel two ends
Two couples totally four detection hole 34；The position that arranges of six electrode holes 21 is respectively as follows: the first electrode hole 21-of corresponding liquid storage tank 31
1, corresponding four the detections second electrode hole 21-2 in hole 34, the 3rd electrode hole 21-3, the 5th electrode hole 21-5, the 6th electrode holes
4th electrode hole 21-4 of the porch of 21-6 and corresponding waste liquid pool 33；Four electrode holes 21 can certainly be only set, inspection
Survey passage two ends each one also possible, such detection data precision can be more slightly worse.Well 22 position pair is set
Answer addition pool 32；Venthole position correspondence waste liquid pool 33 is set.Each electrode hole 21 is at least one stroke of saturating first diaphragm seal 2
Dash line, M shape as shown in Figure 2 or other shape can be divided into；Preset buffer in liquid storage tank 31.First is close
In sealer 2, balance pipe 24 is set between minimum electrode hole 21 and the venthole 23 in position.Venthole 23 is non-circular hole；Or
Venthole 23 and balance pipe 24 are non-circular hole.Liquid storage tank 31 on micro-fluid chip 3, addition pool 32, sense channel and useless
The degree of depth of liquid pool 33 is different.First diaphragm seal 2 is opaque coating.Also it is compounded with opaque second bottom second diaphragm seal 4 to protect
Cuticula 5.First protecting film 1 and the second diaphragm seal 4 are hyaline membrane.The first electrode hole 21-that on first diaphragm seal 2, position is the highest
1 simultaneously as air inlet.
During use, tear the first protecting film 1, each electrode is inserted from electrode hole 21, draw saturating line owing to having, therefore
Electrode inserts very convenient, from well addition, tissue liquid to be detected can be connected detecting instrument and detect.Giving birth to
During product, the second protecting film 5 of first not fitting, owing to the second diaphragm seal 4 is transparent, quality inspection can easily be carried out, quality inspection is passed through,
Fit again the second protecting film 5.
Particular embodiments described above, has been carried out the purpose of the present invention, technical scheme and beneficial effect the most in detail
Describe in detail bright, be it should be understood that the specific embodiment that the foregoing is only the present invention, be not limited to the present invention, all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. done, should be included in the guarantor of the present invention
Within the scope of protecting.
Claims (10)
1. a FCM analysis chip, it is characterised in that: it is compounded with strippable first protecting film the most successively
(1), the first diaphragm seal (2), micro-fluid chip (3) and the second diaphragm seal (4)；Described first diaphragm seal (2) is upper arranges at least four
Individual electrode hole (21), a well (22) and a venthole (23)；According to the use of chip on described micro-fluid chip (3)
Direction is from top to bottom provided with the liquid storage tank (31) of connection, addition pool (32), sense channel and waste liquid pool (33), and is positioned at inspection
Survey at least two detection hole (34) at passage two ends；Corresponding liquid storage tank is distinguished in the position that arranges of described four electrode holes (21)
(31), two detections hole (34) and the porch of waste liquid pool (33)；Described well (22) position correspondence addition pool is set
(32)；Described venthole position correspondence waste liquid pool (33) is set.
A kind of FCM analysis chip the most according to claim 1, it is characterised in that: described each electrode hole (21) is
The dash line of at least one stroke of saturating first diaphragm seal (2)；Preset buffer in described liquid storage tank (31).
A kind of FCM analysis chip the most according to claim 2, it is characterised in that: described electrode hole (21) arranges six
Individual；Described detection hole (34) arranges four, and sense channel two ends respectively arrange two；Upper corresponding four of described first diaphragm seal (2)
Detection hole (34) position respectively sets an electrode hole (21).
A kind of FCM analysis chip the most according to claim 2, it is characterised in that: on described first diaphragm seal (2)
Between electrode hole (21) and venthole (23) that position is minimum, balance pipe (24) is set.
A kind of FCM analysis chip the most according to claim 4, it is characterised in that: described venthole (23) is not rounded
Shape hole；Or described venthole (23) and balance pipe (24) are non-circular hole.
6. according to a kind of FCM analysis chip one of claim 1 to 5 Suo Shu, it is characterised in that: described microfluid core
Liquid storage tank (31) on sheet (3), addition pool (32), sense channel are different with the degree of depth of waste liquid pool (33).
A kind of FCM analysis chip the most according to claim 6, it is characterised in that: described first diaphragm seal (2) is
Opaque coating.
A kind of FCM analysis chip the most according to claim 7, it is characterised in that: described second diaphragm seal (4) end
Portion is also compounded with opaque second protecting film (5).
A kind of FCM analysis chip the most according to claim 7, it is characterised in that: described first protecting film (1) and
Second diaphragm seal (4) is hyaline membrane.
A kind of FCM analysis chip the most according to claim 9, it is characterised in that: on described first diaphragm seal (2)
The highest electrode hole (21) in position is simultaneously as air inlet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610822592.4A CN106198363B (en) | 2016-09-13 | 2016-09-13 | Flow cytometry detection chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610822592.4A CN106198363B (en) | 2016-09-13 | 2016-09-13 | Flow cytometry detection chip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106198363A true CN106198363A (en) | 2016-12-07 |
| CN106198363B CN106198363B (en) | 2020-08-07 |
Family
ID=58066614
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610822592.4A Active CN106198363B (en) | 2016-09-13 | 2016-09-13 | Flow cytometry detection chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106198363B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107213930A (en) * | 2017-07-27 | 2017-09-29 | 深圳中科芯海智能科技有限公司 | A kind of micro-fluidic chip and particle analysis method for particle analysis |
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| CN105510191A (en) * | 2015-11-19 | 2016-04-20 | 江苏卓微生物科技有限公司 | Flow-type particle detection method |
| CN206074412U (en) * | 2016-09-13 | 2017-04-05 | 江苏卓微生物科技有限公司 | A kind of FCM analysis chip |
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2016
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| CN102759466A (en) * | 2004-09-15 | 2012-10-31 | 英特基因有限公司 | Microfluidic devices |
| CN101678356A (en) * | 2007-04-06 | 2010-03-24 | 加利福尼亚技术学院 | Microfluidic device |
| CN102149812A (en) * | 2008-02-21 | 2011-08-10 | 埃凡特拉生物科技公司 | Assays based on liquid flow over arrays |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107213930A (en) * | 2017-07-27 | 2017-09-29 | 深圳中科芯海智能科技有限公司 | A kind of micro-fluidic chip and particle analysis method for particle analysis |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN106198363B (en) | 2020-08-07 |
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Denomination of invention: A Flow Cytometric Detection Chip Effective date of registration: 20230811 Granted publication date: 20200807 Pledgee: China Construction Bank Corporation Changzhou Xinbei sub branch Pledgor: JIANGSU JIMBIO TECHNOLOGY Co.,Ltd. Registration number: Y2023980051702 |