CN106190864A

CN106190864A - A kind of Fusarium 1,281 2

Info

Publication number: CN106190864A
Application number: CN201610592696.0A
Authority: CN
Inventors: 吉民; 李黎; 陈峻青
Original assignee: Southeast University
Current assignee: Southeast University
Priority date: 2015-02-06
Filing date: 2015-02-06
Publication date: 2016-12-07
Also published as: CN104722772A

Abstract

The invention discloses a kind of method utilizing Fusarium 1,281 2 and synthesis Ag/Au composite nanoparticle thereof, said method comprising the steps of: 1 Fusarium 1,281 2 carried out fermentation culture after collect mycelium, it is washed with deionized removal remaining medium, afterwards mycelium is resuspended in sterile deionized water and cultivates, culture fluid is filtered and removes mycelium, obtain the culture supernatant of Fusarium 1,281 2；2 take Fusarium 1,281 2 culture supernatant that obtains in step 1 as response matrix, add silver nitrate solution and chlorauric acid solution carries out reduction reaction, finally centrifugal obtain Ag/Au composite nanoparticle product；Wherein, described Fusarium 1,281 2 deposit number is CCTCC M2014658.The synthetic method environmental protection of the present invention, is the simplest a kind of synthetic method.

Description

A kind of Fusarium 1281-2

Technical field

The application is Application No. 201510064689.9, invention entitled: " utilize Fusarium 1281-2 to synthesize Ag/ The method of Au composite nanoparticle " divisional application, the present invention relates to microorganism and field of nanometer material technology, relate to a kind of fusarium Bacterium (Fusarium sp) 1281-2 strain and the application in field of nanometer material technology thereof.

Background technology

Along with the fast development of nanotechnology, nano material is because of the quantum effect of its uniqueness, small-size effect and big Specific surface area and show distinctive character.Wherein, Nanoalloy material block conjunction owing to its grain size and structure are different from Gold copper-base alloy and show the character of the most excellent uniqueness at aspects such as electricity, magnetic, corrosion stability, catalysis, it has also become nanometer in recent years The research emphasis of Material Field.Alloy material in nano material is a kind of secondary aggregation crystal or noncrystal, wherein for the first time Cohesion is formed nano-particle by metallic atom exactly, and under conditions of keeping fresh surfaces, force together formation by nano-particle Block condensed solids.The pattern of dispersibility that Ag/Au composite nanoparticle is good owing to Nano silver grain has and more rule, Make crystal seed with silver (Ag) nanoparticle, prepare Ag/Au composite nanoparticle.

Ag/Au composite nanoparticle, due to the optical property of its uniqueness, the most also receives much concern, and it has Interesting electricity and structural behaviour, have a wide range of applications at biomedical sector.At present, the preparation Ag/Au of most study is multiple The method closing nanoparticle is chemical reduction method, but this method generally pollutes the environment.Therefore, find preferably, to ring The Ag/Au composite nanoparticle preparation method of border close friend is particularly important.Recently, microorganism system is by as a kind of novelty Method is incorporated in the preparation of inorganic nanoparticles, such as: antibacterial and yeast are used for toxic metals of degrading, biologic artifact It is used for synthesizing various inorganic material.

The Microbe synthesis method of Ag/Au composite nanoparticle, compared with traditional physics and chemical synthesis process, has Lot of advantages, as extensive in material source, cheap, reaction condition is gentle controlled, be a kind of cleaning, nontoxic, environmental friendliness, can The synthetic method of sustainable development, is increasingly becoming the study hotspot in composite nanoparticle synthesis field.Up to now, it has been found that include Multiple species in prokaryote and eukaryote have synthesis Ag/Au composite nanoparticle ability, as single celled antibacterial, Cellulous fungus, is respectively provided with in the intracellular or ability of extracellular synthesis Ag/Au composite nanoparticle.With antibacterial and yeast Comparing, the advantage of fungus is to secrete substantial amounts of enzyme, and separation process is simple, and therefore this field causes what scholars studied Interest.

Summary of the invention

Solved by the invention technical problem is that: providing one to utilize Fusarium 1281-2, this bacterium can apply to green The Ag/Au composite nanoparticle synthesis that colour circle is protected, utilizes synthesis Ag/Au composite nano-granule outside Fusarium culture supernatant vacuole Son, reaction condition is gentle and simple and efficient.

In order to solve above-mentioned technical problem, the technical solution adopted in the present invention is: provide one to utilize Fusarium (Fusarium speciales) 1281-2, preserving number is: CCTCC NO:M2014658.It is preserved in Chinese Typical Representative culture Preservation center, preservation address is: Wuhan, China, Wuhan University；The preservation time is December in 2014 23.

The most visible mycelia has every, branch.Conidiophore branch or not branch, have two kinds of forms: little Type conidium oval, has 1 ~ 2 barrier film；Macroconidium sickleshaped or long cylindricality, have more tabula；Rhizoma Solani tuber osi Well-grown on glucose agar medium, bacterium colony just white, fade to grass green, mycelia is sparse, matrix white.

The invention has the beneficial effects as follows: compared with prior art, advantages of the present invention is as follows: fusarium provided by the present invention Mycete 1281-2, utilizes Fusarium 1281-2 can carry out outside born of the same parents and synthesizes Ag/Au composite nanoparticle, and the Ag/Au of synthesis is combined Nano particle diameter is about 25 ± 2nm, and Ag/Au composite nano-granule molecular dispersivity is higher, independently can disperse, often in water Temperature is lower stable, is a kind of efficient, safety, simple Ag/Au composite nanoparticle synthetic method.

Accompanying drawing explanation

Fig. 1 is that the transmission electron microscope of the Ag/Au composite nanoparticle utilizing Fusarium 1281-2 culture supernatant to synthesize is swept Tracing

Fig. 2 is the scanning electron microscope (SEM) photograph of the Ag/Au composite nanoparticle utilizing Fusarium 1281-2 culture supernatant to synthesize

Fig. 3 is that the EDS of the Ag/Au composite nanoparticle utilizing Fusarium 1281-2 culture supernatant to synthesize can spectrogram

Detailed description of the invention

Below in conjunction with Figure of description, the present invention is further illustrated.

Embodiment 1 utilizes the method for Fusarium 1281-2 synthesis Ag/Au composite nanoparticle

Take frozen Fusarium 1281-2 at potato glucose (PDA) solid medium (containing Rhizoma Solani tuber osi 200 g/L, Fructus Vitis viniferae Sugar 20g/L, agar 15g/L) upper streak inoculation, cultivate 72 h at 28 DEG C, connect mycelium inoculation in 100 mLPDA liquid cultures Base (containing Rhizoma Solani tuber osi 200g/L, glucose 20g/L), 28 DEG C, cultivate 48 h under 150 rpm, collect mycelium.Use deionized water Washing 3 times, by mycelium suspended in deionized water, its concentration is 200g/L, 28 DEG C, cultivate 48h-72h under 120 rpm, Filter and remove mycelium.Collect culture supernatant and add silver nitrate solution and chlorauric acid solution, the concentration of both inorganic salts it Preferably 1mmol/L, in 28 DEG C, place under 120 rpm, it is a little that period takes solution every 6h, and centrifugal collection is combined containing Ag/Au The precipitation of nanoparticle, is washed with deionized 3 times to be suspended into debita spissitudo or dried with deionized water after going the removal of impurity Preserve.

The sign of embodiment 2 Ag/Au composite nanoparticle

Observe the culture supernatant achromaticity and clarification finding the Fusarium 1281-2 before adding silver nitrate solution and chlorauric acid solution, Adding silver nitrate solution and chlorauric acid solution culture fluid after some hours is aubergine, color is along with Ag/Au composite nano-granule Son amount increase and deepen.The transmission electron microscope (TEM) of use resolution 200KV is observed above-mentioned culture supernatant reduction synthesis and is obtained The Ag/Au composite nanoparticle product arrived, display Ag/Au composite nanoparticle form is based on spherical, and particle diameter is that 25 ± 2nm is another The outer Ag/Au compound particle product also having the conformation of rules such as a small amount of triangle, hexagon, dispersibility is higher, it is simple to separate, such as figure Shown in 1.Equally, under scanning electron microscope, visible Ag/Au composite nanoparticle various shapes, multi-angular, and major part is in spherical, such as figure Shown in 2.Fig. 3 is the EDS energy spectrogram that Ag/Au composite nanoparticle is prepared in Fusarium 1281-2 reduction.Power spectrum elemental composition is divided Analysis display, particle prepared by the method for the invention is Ag/Au composite nanoparticle.

The ultimate principle of the present invention, principal character and advantages of the present invention have more than been shown and described.The technology of the industry Personnel, it should be appreciated that the present invention is not restricted to the described embodiments, simply illustrating this described in above-described embodiment and description The principle of invention, without departing from the spirit and scope of the present invention, the present invention also has various changes and modifications, and these become Change and improvement both falls within scope of the claimed invention.Claimed scope by appending claims and Equivalent defines.

Claims

1. a Fusarium, it is characterised in that this bacterium is entitled: Fusarium 1281-2, preserving number is: CCTCC M2014658。

2. being preserved in China typical culture collection center, the preservation time is December in 2014 29, according to claim 1 A kind of Fusarium, this bacterium visible mycelia under an optical microscope has every, branch, conidiophore branch or not branch, has two Kind form: microconidia oval, has 1～2 barrier film；Macroconidium sickleshaped or long cylindricality, have more horizontal stroke Every；Well-grown on potato dextrose agar, bacterium colony just white, fade to grass green, mycelia is sparse, and substrate is white Color.