CN106190109A - Chemiluminescence intensifier and chemiluminescence immune detection reagent kit - Google Patents
Chemiluminescence intensifier and chemiluminescence immune detection reagent kit Download PDFInfo
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Abstract
The invention discloses a kind of chemiluminescence intensifier and chemiluminescence immune detection reagent kit, chemiluminescence intensifier is specially betaines compound, can be used for strengthening the luminous intensity of acridine luminescent substance.Including this chemiluminescence intensifier and the chemiluminescence immune detection reagent kit of acridine luminescent substance, luminous signal is greatly enhanced, detection sensitivity high, can be widely applied to the fields such as clinical diagnosis, scientific research, environmental health detection.
Description
Technical field
The present invention relates to technical field of analysis and detection, particularly relate to a kind of chemiluminescence intensifier and chemiluminescence immunoassay inspection
Test agent box.
Background technology
Chemiluminescence immune assay (Chemiluminescence Immunoassay, CLIA) is by chemiluminescence or life
Thing luminescence system combines with immunoreation, for detecting a kind of novel markings immunoassay of trace antigen or antibody.
Chemiluminescence immune assay is the immunoassay technology of main flow in current world wide, have highly sensitive, detection range width,
Response time is short, full automatic working, favorable reproducibility, the advantage such as pollution-free, be to continue to put immune technology, enzyme immune technology, fluorescence immunoassay skill
The supersensitive Micrometry of one grown up after art.Chemiluminescent principle is when ground state molecule absorptionization
Learn in reaction the energy jump of release to excited state, be in generation when the molecule of excited state returns to ground state with the form of light radiation
Light.Chemiluminescence is combined by chemiluminescence immune assay with immune analysis method, combines chemiluminescent high sensitivity
High selectivity with immunoassay.
In chemiluminescence immune assay, conventional luminescent substance includes luminol, different luminol, 3-(2-spiral Buddha's warrior attendant
Alkane)-4-methoxyl group-4-(3-phosphorus oxygen acyl)-phenyl-1,2-dioxane disodium salt (being abbreviated as AMPPD), tris (bipyridine) ruthenium and
Acridine luminescent substance.Wherein, different luminol, tris (bipyridine) ruthenium and acridine luminescent substance directly can be marked as tracer molecule
Note, belongs to flash type chemiluminescent substance.Luminol and AMPPD rely on peroxidase and alkali phosphatase as spike respectively
Molecular marker, belongs to enzymatic light emitting-type chemiluminescent substance.The luminescence-producing reaction of acridine class luminescent substance has and is not required to catalyst,
As long as can be carried out in alkaline environment, and luminescence-producing reaction is rapid, and background luminescence is low, and signal to noise ratio is high.Luminescence-producing reaction interference because of
Element is few, and reagent stability is good, and chemical luminous system is simple, and exciting liquid low cost and other advantages, is preferable chemiluminescent substance.
Traditional acridine Full-automatic chemiluminescence system mainly uses hydrogen peroxide and acid solution and alkaline solution
Coordinating and start reagent as luminous, some adds reinforcing agent Triton X-100 (Triton in luminescence starts reagent
X-100) and tween 20 (Tween 20) is to strengthen luminescence, but traditional reinforcing agent strengthens the effect of luminescence not after adding
Substantially.In sum, the luminous intensity of traditional acridine luminescent substance is more weak.
Summary of the invention
Based on this, it is necessary to provide the chemiluminescence intensifier of a kind of luminous intensity that can strengthen acridine luminescent substance
And chemiluminescence immune detection reagent kit.
A kind of chemiluminescence intensifier, for strengthening the luminous intensity of acridine luminescent substance, described chemiluminescent enhancement
Agent includes betaines compound.
In one embodiment, the structural formula of described betaines compound is:
Wherein, R-For anionic group, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or
C2～C10Alkynyl, R2For C1～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1
～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.
In one embodiment, the structural formula of described betaines compound is:
Wherein, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2
For C1～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1~
C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.
In one embodiment, the structural formula of described betaines compound is:
Wherein, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2
For C1～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1~
C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.
In one embodiment, the structural formula of described betaines compound is:
Wherein, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2
For C1～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1~
C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.
In one embodiment, the structural formula of described betaines compound is:
Wherein, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2
For C1～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1~
C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.
In one embodiment, the structural formula of described betaines compound is:
Wherein, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2
For C1～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1~
C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.
A kind of chemiluminescence immune detection reagent kit, including the chemiluminescence intensifier described in any of the above-described item and acridine
Class luminescent substance.
In one embodiment, described acridine luminescent substance is acridinium ester or acridinium salt.
In one embodiment, chemiluminescence preexciting liquid and exciting liquid, described chemiluminescence preexciting liquid are also included
Including HNO3And H2O2Solution, exciting liquid includes NaOH solution.
Betaines compound is the compound with quatemay ammonium inner salt structure or referred to as ammonium structure, is commonly used for osmotic pressure and adjusts
Joint agent, cleaning agent or antibacterial etc..Inventors be surprised to learn that betaines compound has and strengthen sending out of acridine luminescent substance
The effect of light intensity, can be used for preparing chemiluminescence intensifier.Experiment shows, this betaines compound can be remarkably reinforced acridine
The luminous intensity of class luminescent substance, and the multiple strengthened is higher than traditional reinforcing agent Triton X-100 and Tween 20.
Accompanying drawing explanation
Fig. 1 adds containing myristamide propyl group hydroxypropyl for using iFlash 3000 Full-automatic chemiluminescence apparatus to measure
The intensity profile of light emission figure of acridinium ester after the chemiluminescence intensifier of sulfobetaines；
Fig. 2 for use use iFlash 3000 Full-automatic chemiluminescence apparatus measure without reinforcing agent be separately added into chemistry
The luminescence of acridinium salt under the conditions of luminescence enhancer and Architect Trigger Solution (Abbott) commodity luminescent solution
Intensity.
Detailed description of the invention
Understandable for enabling the above-mentioned purpose of the present invention, feature and advantage to become apparent from, real with concrete below in conjunction with the accompanying drawings
Execute example the detailed description of the invention of the present invention is described in detail.Elaborate a lot of detail in the following description so that
Fully understand the present invention.But the present invention can implement to be much different from alternate manner described here, art technology
Personnel can do similar improvement in the case of intension of the present invention, and therefore the present invention is not embodied as by following public
Restriction.
The chemiluminescence intensifier of one embodiment, this chemiluminescence intensifier includes betaines compound, this chemistry
Luminescence enhancer can be used for strengthening the luminous intensity of acridine luminescent substance.Certainly, chemiluminescence intensifier can also include molten
Solve the solvent of betaines compound or acceptable impurity etc..
Betaines compound is the compound with quatemay ammonium inner salt structure or referred to as ammonium structure.Acridine luminescent substance
It is the class chemical substance that can be used as chemiluminescent labels, such as acridinium ester or acridinium salt etc..
Concrete, the structural formula of betaines compound is:
Wherein, R-For anionic group, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or
C2～C10Alkynyl, R2For C1～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1
～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.
R-For anionic group, form betaines compound with quatemay ammonium inner salt respective outer side edges.
In one embodiment, betaines compound is compounds I, and the structural formula of compounds I is:
Wherein, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2
For C1～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1~
C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.I.e. in present embodiment ,-R-For-COO-。
In another embodiment, betaines compound is compound ii, and the structural formula of compound ii is:
Wherein, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2
For C1～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1~
C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.I.e. in present embodiment ,-R-For
In another embodiment, betaines compound is compound III, and the structural formula of compound III is:
Wherein, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2
For C1～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1~
C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.I.e. in present embodiment ,-R-For-CH2-SO3 -。
In a further embodiment, betaines compound is compounds Ⅳ, and the structural formula of compounds Ⅳ is:
Wherein, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2
For C1～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1~
C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.I.e. in present embodiment ,-R-For-CH2-CH2-SO3 -。
In a further embodiment, betaines compound is compound V, and the structural formula of compound V is:
Wherein, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2
For C1～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1~
C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.I.e. in present embodiment ,-R-For-CH (OH)-CH2-SO3 -。
In this article, for special instruction, the C in each structural formula5～C20Alkyl can be straight chained alkyl or branched alkyl.
C2～C10Thiazolinyl can be straight-chain alkenyl or branched-chain alkenyl.C2～C10Alkynyl can be straight-chain alkynyl or branch alkynyl.C1
～C10Alkyl can be straight chained alkyl or branched alkyl.C1～C10Alkoxyl can be straight chained alkyl or branched alkyl.
Further, R1For C5～C20Alkylamidoalkyl, C10～C20Alkyl, R2For C1～C5Alkyl, C1～C5's
Alkoxyl, C2～C5Thiazolinyl or C2～C5Alkynyl.R3For C1～C5Alkyl, C1～C5Alkoxyl, C2～C5Thiazolinyl or
C2～C5Alkynyl.
Concrete, alkylamidoalkyl refers to the group that alkyl is connected with amide groups, and its structural formula can be such as C5H11-
CONH-、C11H24-CONH-、C13H27-CONH-or C20H27-CONH-etc..Further, the amide groups in alkylamidoalkyl is also
C can be connected1～C10Alkyl, its structural formula can be such as C13H27-CONH-(CH2)3-or C11H24-CONH-(CH2)10-
Deng.
Concrete, R1Can be C5、C6、C7、C8、C9、C10、C11、C12、C13、C14、C15、C16、C17、C18、C19Or C20Alkane
Base.R1Can also be C2、C3、C4、C5、C6、C7、C8、C9Or C10Thiazolinyl.Or, R1Can also be C2、C3、C4、C5、C6、C7、
C8、C9Or C10Alkynyl.
Concrete, R2Can be C1、C2、C3、C4、C5、C6、C7、C8、C9Or C10Alkyl.R2Can be C1、C2、C3、C4、
C5、C6、C7、C8、C9Or C10Alkoxyl.R2Can also be C2、C3、C4、C5、C6、C7、C8、C9、C10Thiazolinyl.Or, R2Also may be used
Think C2、C3、C4、C5、C6、C7、C8、C9Or C10Alkynyl.
Concrete, R3Can be C1、C2、C3、C4、C5、C6、C7、C8、C9Or C10Alkyl.R3Can be C1、C2、C3、C4、
C5、C6、C7、C8、C9Or C10Alkoxyl.R3Can also be C2、C3、C4、C5、C6、C7、C8、C9、C10Thiazolinyl.Or, R3Also may be used
Think C2、C3、C4、C5、C6、C7、C8、C9Or C10Alkynyl.
Further, R1、R2And R3Can be the same or different.Work as R1、R2And R3When being respectively selected from alkenyl or alkynyl, alkene
Base or alkynyl can optional positions in corresponding group.
Inventors be surprised to learn that betaines compound has the effect of the luminous intensity strengthening acridine luminescent substance, can
For preparing chemiluminescence intensifier.Betaines compound has the character of amphoteric surfactant, simple in construction, composition list
One, difference between batch is very easy to control, and effectively reduces the different shadow of the chemiluminescent enhancement effect brought due to batch wise differences
Ring.By this chemiluminescence intensifier, can effectively strengthen the luminous intensity of acridine luminescent substance, luminous signal is remarkably reinforced,
And favorable reproducibility, can be widely applied to the fields such as clinical diagnosis, scientific research, environmental health detection.Further, on having
The luminous intensity of acridine luminescent substance can be strengthened more than at least twice by the compound stating formula, and the multiple of enhancing is high
In traditional reinforcing agent Triton X-100 and Tween 20.
A kind of chemiluminescence immune detection reagent kit, including above-mentioned chemiluminescence intensifier and acridine luminescent substance.
General, betaines compound dissolution can be made chemiluminescence intensifier in a solvent.Solvent can be water,
Acid solution or alkaline solution etc..In present embodiment, betaines compound dissolution is made chemiluminescent enhancement in water
Agent.
Concrete, acridine luminescent substance can be acridinium ester.Such as NSP-DMAE-NHS acridinium ester, NSP-DMAE-
HEG-NHS acridinium ester etc..
The chemiluminescence reaction procedural style of the acridine luminescent substance of one embodiment is as follows:
Wherein, the acridine compound of representation shown in formula 1, the R in formula 1～formula 6 can be H, alkyl or alkyl sulphur
Bases etc., Ar can be the group containing phenyl.Concrete luminescence process is: the acridine compound shown in formula 1 is in acid condition
By 9 carbon atoms of hydrogen peroxide addition acridine ring, obtain the compound shown in formula 2, generated the most in the basic conditions
Negative oxygen ion, obtains the compound shown in formula 3.Afterwards by intramolecular attack carbonyl, leaving group is left away, and generates in instability
Mesosome 1,2-dioxetanes alkanone (compound shown in formula 6), then 1,2-dioxetanes alkanone open loop generates acridone,
Discharge photon simultaneously.
General, in chemiluminescence immune detection reagent kit, chemiluminescence intensifier and acridine luminescent substance are point
Opening is put, and remixes during use.
In present embodiment, chemiluminescence immune detection reagent kit also includes chemiluminescence preexciting liquid and exciting liquid, changes
Learn luminous preexciting liquid and include HNO3And H2O2Solution, exciting liquid is NaOH solution.
Concrete, HNO3The concentration of solution is 0.05mol/L～0.3mol/L, H2O2The volumn concentration of solution is
0.1%～1%.The concentration of NaOH solution is 0.05mol/L～0.5mol/L.
In present embodiment, HNO3The concentration of solution is 0.1mol/L, H2O2The volumn concentration of solution is 0.5%.
The concentration of NaOH solution is 0.25mol/L.
Chemiluminescence preexciting liquid and basis exciting liquid, as luminous startup reagent, coordinate with chemiluminescence intensifier,
Improve the luminous intensity of pyridine class luminescent substance further.
Concrete, chemiluminescence intensifier can be added in the exciting liquid of basis and prepare chemi-excitation liquid, thus improve pyridine class
The luminous intensity of luminescent substance.
Above-mentioned chemiluminescence immune detection reagent kit, including chemiluminescence intensifier and acridine luminescent substance.By Radix Betae
Alkaloid compound, as chemiluminescence intensifier, can effectively strengthen the luminous intensity of acridine luminescent substance, and the multiple of enhancing is high
In traditional reinforcing agent Triton X-100 and Tween 20, and favorable reproducibility.And betaines compound has both sexes
The character of surfactant, simple in construction, composition are single, and difference between batch is very easy to control, and effectively reduces due to batch wise differences
The different impact of the chemiluminescent enhancement effect brought, improves chemiluminescence immune detection reagent kit detection sensitivity, this examination
Agent box can be widely applied to the fields such as clinical diagnosis, scientific research, environmental health detection.
It it is below embodiment part.
In following example, if no special instructions, the experimental technique of unreceipted actual conditions, generally according to normal condition,
Experiment material betaines compound used is purchased from Henan Titaning Chemical Technology Co., Ltd..Tween20 (sigma company, goods
Number V900548) and Triton X-100 (sigma company, article No. V900502).Acridinium ester (NSP-DMAE-NHS) and acridinium salt
(NSP-SA-NHS) purchase to step in Shanghai and open up chemical industry of rivering bend.Detecting chemiluminescent instrument is that iFlash 3000 full-automatic chemical is sent out
Light instrument.
Embodiment 1～embodiment 13
The impact on the luminous intensity of acridine luminescent substance of the betaines compound
1) configuration preexciting liquid
With ultra-pure water configuration containing HNO3And H2O2Preexciting liquid, wherein HNO3Final concentration of in preexciting liquid
0.1mol/L, H2O2Solution volumn concentration in preexciting liquid is 0.5%.
2) configuration basis exciting liquid
With the ultra-pure water configuration basic exciting liquid containing NaOH, the NaOH final concentration of 0.25mol/ in the exciting liquid of basis
L。
3) configuration chemi-excitation liquid
Being separately added into chemiluminescence intensifier in the exciting liquid of basis, chemiluminescence intensifier includes betaines respectively
Compound Z1～Z13, obtains chemi-excitation liquid.The original content of each betaines compound Z1～Z13 in chemiluminescence intensifier
Being 10g/L, chemiluminescence intensifier addition is 2 μ L～30 μ L.Be separately added into 10 μ L in the present embodiment contains Z1 respectively
～the chemiluminescence intensifier of Z13 obtains chemi-excitation liquid in the exciting liquid of basis.The glycine betaine that chemiluminescence intensifier includes
The structural formula of compounds Z1～Z13 is as follows:
Wherein, R-For anionic group, specifically having following Y1～Y5 tetra-class, Y1～Y5 concrete structure is as follows:
R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2For C1
～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1～C10
Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.Concrete structure such as table corresponding for betaines compound Z1～Z13
Shown in 1.
Table 1: the title of betaines compound Z1～Z13 and structural formula
4) mensuration of chemiluminescence signal
Acridine solution with 10 μ L, concentration as 1pg/mL is as sample, and in the present embodiment, acridine solution is NSP-DMAE-HEG-
NHS acridinium ester, its structural formula is as follows:
In sample, add 100 μ L preexciting liquid, then be separately added into 100 μ L each chemi-excitation liquid.Chemi-excitation liquid divides
Do not contain in the exciting liquid of basis and betaines compound Z1～Z13.Chemiluminescence detector is measured chemiluminescence letter
Number, and calculate the intensification factor of each luminescent solution signal value and basic luminescence liquid signal value.The luminosity curve of embodiment 5 correspondence such as figure
Shown in 1.The luminous intensity result of embodiment 1～13 is as shown in table 2.
Table 2: experiment contrast group and the luminous intensity of embodiment 1～13
Wherein, experiment contrast group is except being not added with chemiluminescence intensifier, and remaining is identical with embodiment 1～13.Permissible from table 2
Find out, add the chemiluminescence intensifier containing betaines compound Z1～Z13 and can effectively strengthen the luminescence of acridinium ester
Intensity.Intensification factor is more than 4 times.
Comparative example 1～comparative example 2
For the checking betaines compound impact on the luminous intensity of acridinium ester further.Use Tween 20 He
Triton X-100 is as a comparison case.Comparative example configures preexciting liquid and step and the embodiment 1 of configuration basis exciting liquid～
13 is identical.Except for the difference that, during configuration chemi-excitation liquid, use respectively by the Tween that original content is 10g/L 20 He of 10 μ L
Triton X-100 adds the chemi-excitation liquid obtaining comparative example in the exciting liquid of basis.It is right to measure on employing chemiluminescence detector
The chemiluminescence signal of ratio, and calculate the intensification factor of each luminescent solution signal value and basic luminescence liquid signal value, result such as table 3
Shown in.
Table 3: experiment contrast group and the luminous intensity of comparative example 1 and 2
The result of contrast table 2 and table 3 understands, and uses betaines compound can play enhancing acridine compound chemistry and sends out
Light action, and reinforced effects is better than Tween 20 and Triton X-100.And the chemiluminescence of the present embodiment 1～13 is the most luminous
Signal stabilization, luminous efficiency is high.
Embodiment 14
For comparing betaines compound further to acridine compound the enhancement of chemiluminescence, selection
Architect Trigger Solution (Abbott company, article No.: 6C55-60) commodity luminescent solution is as comparison.This enforcement
The sample liquid of example be 10 μ L concentration be acridinium salt (NSP-SA-NHS) solution of 1pg/mL.Preexciting liquid and basis exciting liquid and reality
Execute example 1～13 identical.It is added to the chemiluminescence intensifier containing Z5 of 10 μ L in the exciting liquid of basis obtain chemi-excitation liquid.To
Sample adds 100 μ L preexciting liquid, then is separately added into the chemi-excitation liquid corresponding containing reinforcing agent Z5 or the 100 μ L of 100 μ L
Architect Trigger Solution (Abbott) commodity luminescent solution.Measure respectively without reinforcing agent, have containing Z5's
Acridinium salt under the conditions of chemiluminescence intensifier and Architect Trigger Solution (Abbott) commodity luminescent solution
Luminous intensity.Result as in figure 2 it is shown, with commodity luminescent solution Architect Trigger Solution (Abbott) luminous signal
Contrast.Under reinforcing agent effect of the present invention, the luminous signal of acridine is greatly enhanced, and strengthens luminescent solution performance close
Architect Trigger Solution(Abbott)。
From embodiment 1～14 and contrast 1 result knowable to, acridine compound is had well by betaines compound
The effect of enhanced chemiluminescence, can improve the detection sensitivity of diagnostic kit.
All data, image, instrument, reagent and step herein are interpreted as illustrative and not restrictive.Although
Combined above-mentioned specific embodiments and described the present invention, many amendments and other change for those skilled in the art be aobvious and
It is clear to.All this amendments and other change can fall within the spirit and scope of the present inventions.
Embodiment described above only have expressed the several embodiments of the present invention, and it describes more concrete and detailed, but also
Therefore the restriction to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that, for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, it is also possible to make some deformation and improvement, these broadly fall into the guarantor of the present invention
Protect scope.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. a chemiluminescence intensifier, for strengthening the luminous intensity of acridine luminescent substance, it is characterised in that described chemistry
Luminescence enhancer includes betaines compound.
Chemiluminescence intensifier the most according to claim 1, it is characterised in that the structural formula of described betaines compound
For:
Wherein, R-For anionic group, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2~
C10Alkynyl, R2For C1～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1~
C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.
Chemiluminescence intensifier the most according to claim 2, it is characterised in that the structural formula of described betaines compound
For:
Wherein, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2For C1
～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1～C10
Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.
Chemiluminescence intensifier the most according to claim 2, it is characterised in that the structural formula of described betaines compound
For:
Wherein, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2For C1
～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1～C10
Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.
Chemiluminescence intensifier the most according to claim 2, it is characterised in that the structural formula of described betaines compound
For:
Wherein, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2For C1
～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1～C10
Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.
Chemiluminescence intensifier the most according to claim 2, it is characterised in that the structural formula of described betaines compound
For:
Wherein, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2For C1
～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1～C10
Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.
Chemiluminescence intensifier the most according to claim 2, it is characterised in that the structural formula of described betaines compound
For:
Wherein, R1For C5～C20Alkylamidoalkyl, C5～C20Alkyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R2For C1
～C10Alkyl, C1～C10Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl, R3For C1～C10Alkyl, C1～C10
Alkoxyl, C2～C10Thiazolinyl or C2～C10Alkynyl.
8. a chemiluminescence immune detection reagent kit, it is characterised in that include the change as described in any one of claim 1～7
Learn luminescence enhancer and acridine luminescent substance.
Chemiluminescence immune detection reagent kit the most according to claim 8, it is characterised in that described acridine luminescent substance
For acridinium ester or acridinium salt.
Chemiluminescence immune detection reagent kit the most according to claim 8, it is characterised in that also include that chemiluminescence is pre-
Exciting liquid and exciting liquid, described chemiluminescence preexciting liquid includes HNO3And H2O2Solution, exciting liquid includes NaOH solution.
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| JPS5851187A (en) * | 1981-09-19 | 1983-03-25 | Ricoh Co Ltd | Diazo heat-sensitive recording material |
| US5215976A (en) * | 1991-10-28 | 1993-06-01 | Mona Industries, Inc. | Phospholipids useful as spermicidal agents |
| CN102391538A (en) * | 2011-08-26 | 2012-03-28 | 华东理工大学 | Pre-modified PVC (Polyvinyl Chloride) resin and modified PVC (Polyvinyl Chloride) resin as well as preparation method thereof and application thereof |
| CN104891570A (en) * | 2015-04-19 | 2015-09-09 | 宁波大学 | Liquid phase synthetic Zr<4+> doped bismuth fluoride lithium-ion battery positive electrode material and preparation method thereof |
| CN105623637A (en) * | 2014-11-10 | 2016-06-01 | 中国石油化工股份有限公司 | Alkali-free working fluid protection system and usage method thereof |
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2016
- 2016-07-01 CN CN201610519315.6A patent/CN106190109B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5851187A (en) * | 1981-09-19 | 1983-03-25 | Ricoh Co Ltd | Diazo heat-sensitive recording material |
| US5215976A (en) * | 1991-10-28 | 1993-06-01 | Mona Industries, Inc. | Phospholipids useful as spermicidal agents |
| CN102391538A (en) * | 2011-08-26 | 2012-03-28 | 华东理工大学 | Pre-modified PVC (Polyvinyl Chloride) resin and modified PVC (Polyvinyl Chloride) resin as well as preparation method thereof and application thereof |
| CN105623637A (en) * | 2014-11-10 | 2016-06-01 | 中国石油化工股份有限公司 | Alkali-free working fluid protection system and usage method thereof |
| CN104891570A (en) * | 2015-04-19 | 2015-09-09 | 宁波大学 | Liquid phase synthetic Zr<4+> doped bismuth fluoride lithium-ion battery positive electrode material and preparation method thereof |
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Address after: 518116 Yahui Long Biotechnology Plant, Baolong Street, Longgang District, Shenzhen City, Guangdong Province Applicant after: SHENZHEN YHLO BIOTECH Co.,Ltd. Address before: 518000 Guangdong, Shenzhen, Nanshan District Nanshan street, Xinghai Road, Li Shan Industrial Zone, 1-4 1-4 floors. Applicant before: SHENZHEN YHLO BIOTECH Co.,Ltd. |
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Application publication date: 20161207 Assignee: Hunan Yahuilong Biotechnology Co.,Ltd. Assignor: SHENZHEN YHLO BIOTECH Co.,Ltd. Contract record no.: X2022440020010 Denomination of invention: Chemiluminescence Enhancer and Chemiluminescence Immunoassay Kit Granted publication date: 20190125 License type: Common License Record date: 20220823 |