CN106177943A - A kind of adjuvant for infectious bronchitis vaccines - Google Patents
A kind of adjuvant for infectious bronchitis vaccines Download PDFInfo
- Publication number
- CN106177943A CN106177943A CN201610684311.3A CN201610684311A CN106177943A CN 106177943 A CN106177943 A CN 106177943A CN 201610684311 A CN201610684311 A CN 201610684311A CN 106177943 A CN106177943 A CN 106177943A
- Authority
- CN
- China
- Prior art keywords
- adjuvant
- infectious bronchitis
- radix astragali
- astragali extract
- alumen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55505—Inorganic adjuvants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55511—Organic adjuvants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55588—Adjuvants of undefined constitution
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/20011—Coronaviridae
- C12N2770/20034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
Abstract
The invention discloses a kind of adjuvant for infectious bronchitis vaccines, Alumen, vitamin E, Radix Astragali extract, lanoline are organically combined by the present invention, coordinate mutually, prepare the immunity composite adjuvant for infectious bronchitis vaccines, play the sharpest edges of adjuvant, share immune effect preferably with antigen, toxicity is little, low cost.Radix Astragali extract therein can enhancing body specific humoral immunity and promote the growth of central immune organ, thus strengthen the immunologic function of chicken.
Description
Technical field
The present invention relates to vaccine adjuvant technical field, particularly relate to a kind of assistant for infectious bronchitis vaccines
Agent.
Background technology
Adjuvant is nonspecific immunity strengthening agent, when being used together with antigen or being previously implanted body, and can enhancing body
Immunne response or change type of immune response to antigen.It is the physics by changing antigen that adjuvant strengthens the mechanism of immunne response
Shape, extends antigen retention time in body；Stimulate the mononuclear phagocyte submission ability to antigen；Lymphocyte is stimulated to divide
Change, increase and expand immunne response ability.
Adjuvant is initially to be described as a kind of material with specific antigen use in conjunction by Gaston Ramon
, and with the specific antigen of adjuvant use in conjunction now produced immunne response when being used alone than it much higher.One
For as, adjuvant itself does not provide immunity, and only when preparation a kind of with immunogen composition, they can improve by vaccine
The range of the immunne response that induction produces and effect.Existing immunological adjuvant, because weak effect or virulence are big, especially lacks
Specific aim, does not has one to obtain promising result.Therefore it is an object of the invention to provide a kind of for infectious bronchitis of chicken
The immunologic adjuvant of vaccine.
Summary of the invention
The purpose of the present invention is aiming at the defect of above-mentioned existence and provides a kind of for infectious bronchitis vaccines
Adjuvant, the present invention by Alumen, vitamin E, Radix Astragali extract, lanoline organically combine, coordinate mutually, prepare for chicken pass
The immunity composite adjuvant of metachromia bronchitis vaccine, plays the sharpest edges of adjuvant, share immune effect preferably with antigen, toxicity
Little, low cost.Radix Astragali extract therein can enhancing body specific humoral immunity and promote central immune organ growth, from
And strengthen the immunologic function of chicken.
A kind of adjuvant technical scheme for infectious bronchitis vaccines of the present invention is, including Alumen, vitamin
E, lanoline, Radix Astragali extract.
Described a kind of adjuvant for infectious bronchitis vaccines, the following component including by weight: Alumen
6-15, vitamin e1-5, lanoline 2-6, Radix Astragali extract 0.1-2.
Described a kind of adjuvant for infectious bronchitis vaccines, the following component including by weight: Alumen
8-10, vitamin E2-4, lanoline 3-5, Radix Astragali extract 0.5-1.5.
Described a kind of adjuvant for infectious bronchitis vaccines, the following component including by weight: Alumen
9, vitamin E 3, lanoline 4, Radix Astragali extract 1.
Described Radix Astragali extract contains astragaloside by weight: 0.5%-40%, astragalus polysaccharides: 20-70%.
Described Radix Astragali extract contains astragaloside by weight: 32%, astragalus polysaccharides: 50%.
Described a kind of adjuvant for infectious bronchitis vaccines, preparation method is:
(1) Alumen is configured to the aluminum potassium sulfate solution of 10-20%；
(2) vitamin E, Radix Astragali extract, lanoline are added in aluminum potassium sulfate solution, mix homogeneously, adjust pH to be helped to 6.5
Agent.
In step (1), Alumen is configured to the aluminum potassium sulfate solution of 12%.
Antigen is added in adjuvant according to the weight ratio of 1:1, according to 2500rpm, emulsifying 30 minutes.
The invention have the benefit that Alumen, vitamin E, Radix Astragali extract, lanoline are organically combined, mutually by the present invention
Coordinate mutually, prepare the immunity composite adjuvant for infectious bronchitis vaccines, play the sharpest edges of adjuvant, with antigen
Share immune effect preferable, toxicity is little, low cost.Radix Astragali extract therein can enhancing body specific humoral immunity and promotion
The growth of central immune organ, thus strengthen the immunologic function of chicken；Radix Astragali extract can improve intestinal structure, strengthens small intestinal and inhales
Receipts, disease-resistant function, thus promote the growth promoter of chicken, improve intestinal immunity.
The present invention each composition low cost, effective simultaneously, the adjuvant being combined into and infectious bronchitis vaccines antigen
In conjunction with, form optimal formula, i.e. form immunostimulating complex, preferably play immunological adjuvant effect.Apply this adjuvant and
It is used alone adsorbed onto alum adjuvant or uses existing Freund adjuvant, be respectively configured the immunity of infectious bronchitis vaccines live vaccine
Animal, applies its antibody titer of adjuvant group of the present invention obviously higher than being used alone adsorbed onto alum adjuvant or using existing Freund to help
Agent experimental group, exceeds about 10 antibody titers.
Detailed description of the invention:
In order to be more fully understood that the present invention, describe technical scheme in detail with instantiation below, but the present invention
It is not limited thereto.
Embodiment 1
Described a kind of adjuvant for infectious bronchitis vaccines, the following component including by weight: Alumen 9, dimension
Raw element E3, lanoline 4, Radix Astragali extract 1.
Described Radix Astragali extract contains astragaloside by weight: 32%, astragalus polysaccharides: 50%.
Preparation method is:
(1) Alumen is configured to the aluminum potassium sulfate solution of 12%；
(2) vitamin E, Radix Astragali extract, lanoline are added in aluminum potassium sulfate solution, mix homogeneously, adjust pH to be helped to 6.5
Agent.
Antigen is added in adjuvant according to the weight ratio of 1:1, according to 2500rpm, emulsifying 30 minutes, obtains vaccine.
According to national basic veterinary drug standard, verify its result:
Safety verification: with Beijing Cold boiled chicken 10 of 28-35 age in days, every collunarium, eye dripping vaccine 0.05ml.Observe 3 weeks interior 10
All there is not any untoward reaction in chicken.
Efficacy test: immune group healthy Beijing Cold boiled chicken 10 at 1 to 2 monthly age, every collunarium, eye dripping vaccine
0.05ml；Healthy Beijing Cold boiled chicken 5 of matched group the same terms, does not carry out immunity.
After immunity 3 weeks, attacking all chickens with infectious bronchitis virus IBV, every chicken intratracheal injection 0.5ml is sick
Toxogen liquid, observes about 10 days.
All there is obvious respiratory symptom in comparison chicken, shows as serious moist sound, and another spiritedness is depressed, subtract food, draw
The phenomenon of yellow loose stool.
All there is not moist sound in immunity chicken, and protective rate is 100%.
Embodiment 2
Described a kind of adjuvant for infectious bronchitis vaccines, the following component including by weight: Alumen 10, dimension
Raw element E2, lanoline 5, Radix Astragali extract 1.5.
Described Radix Astragali extract contains astragaloside by weight: 40%, astragalus polysaccharides: 20%.
Preparation method is:
(1) Alumen is configured to the aluminum potassium sulfate solution of 15%；
(2) vitamin E, Radix Astragali extract, lanoline are added in aluminum potassium sulfate solution, mix homogeneously, adjust pH to be helped to 6.5
Agent.
Antigen is added in adjuvant according to the weight ratio of 1:1, according to 2500rpm, emulsifying 30 minutes, obtains vaccine.
According to national basic veterinary drug standard, verify its result:
Safety verification: with Beijing Cold boiled chicken 10 of 28-35 age in days, every collunarium, eye dripping vaccine 0.05ml.Observe 3 weeks interior 10
All there is not any untoward reaction in chicken.
Efficacy test: immune group healthy Beijing Cold boiled chicken 10 at 1 to 2 monthly age, every collunarium, eye dripping vaccine
0.05ml；Healthy Beijing Cold boiled chicken 5 of matched group the same terms, does not carry out immunity.
After immunity 3 weeks, attacking all chickens with infectious bronchitis virus IBV, every chicken intratracheal injection 0.5ml is sick
Toxogen liquid, observes about 10 days.
All there is obvious respiratory symptom in comparison chicken, shows as serious moist sound, and another spiritedness is depressed, subtract food, draw
The phenomenon of yellow loose stool.
All there is not moist sound in immunity chicken, and protective rate is 100%.
Claims (8)
1. the adjuvant for infectious bronchitis vaccines, it is characterised in that include Alumen, vitamin E, lanoline,
Radix Astragali extract.
A kind of adjuvant for infectious bronchitis vaccines the most according to claim 1, it is characterised in that include by
The following component of weight ratio: Alumen 6-15, vitamin e1-5, lanoline 2-6, Radix Astragali extract 0.1-2.
A kind of adjuvant for infectious bronchitis vaccines the most according to claim 1, it is characterised in that include by
The following component of weight ratio: Alumen 8-10, vitamin E2-4, lanoline 3-5, Radix Astragali extract 0.5-1.5.
A kind of adjuvant for infectious bronchitis vaccines the most according to claim 1, it is characterised in that include by
The following component of weight ratio: Alumen 9, vitamin E 3, lanoline 4, Radix Astragali extract 1.
A kind of adjuvant for infectious bronchitis vaccines the most according to claim 1, it is characterised in that described
Radix Astragali extract contains astragaloside by weight: 0.5%-40%, astragalus polysaccharides: 20-70%.
A kind of adjuvant for infectious bronchitis vaccines the most according to claim 5, it is characterised in that described
Radix Astragali extract contains astragaloside by weight: 32%, astragalus polysaccharides: 50%.
A kind of adjuvant for infectious bronchitis vaccines the most according to claim 1, it is characterised in that preparation side
Method is:
(1) Alumen is configured to the aluminum potassium sulfate solution of 10-20%；
(2) vitamin E, Radix Astragali extract, lanoline are added in aluminum potassium sulfate solution, mix homogeneously, adjust pH to be helped to 6.5
Agent.
A kind of adjuvant for infectious bronchitis vaccines the most according to claim 7, it is characterised in that step
(1), in, Alumen is configured to the aluminum potassium sulfate solution of 12%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610684311.3A CN106177943A (en) | 2016-08-18 | 2016-08-18 | A kind of adjuvant for infectious bronchitis vaccines |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610684311.3A CN106177943A (en) | 2016-08-18 | 2016-08-18 | A kind of adjuvant for infectious bronchitis vaccines |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106177943A true CN106177943A (en) | 2016-12-07 |
Family
ID=57521782
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610684311.3A Pending CN106177943A (en) | 2016-08-18 | 2016-08-18 | A kind of adjuvant for infectious bronchitis vaccines |
Country Status (1)
| Country | Link |
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| CN (1) | CN106177943A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102245201A (en) * | 2008-10-12 | 2011-11-16 | 麻省理工学院 | Adjuvant incorporation in immunonanotherapeutics |
| CN104667272A (en) * | 2015-01-22 | 2015-06-03 | 宿迁恒瑞生物科技有限公司 | Animal immune adjuvant as well as preparation method and application method thereof |
-
2016
- 2016-08-18 CN CN201610684311.3A patent/CN106177943A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102245201A (en) * | 2008-10-12 | 2011-11-16 | 麻省理工学院 | Adjuvant incorporation in immunonanotherapeutics |
| CN104667272A (en) * | 2015-01-22 | 2015-06-03 | 宿迁恒瑞生物科技有限公司 | Animal immune adjuvant as well as preparation method and application method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 吕殿红等: ""复方免疫增强剂对鸡新城疫和传染性腔上囊病疫苗免疫效果的影响"", 《中国兽医科技》 * |
| 王永芬等: "《动物生物制品技术》", 31 August 2011 * |
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| RJ01 | Rejection of invention patent application after publication | ||
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Application publication date: 20161207 |