CN106173501A - Improve the feedstuff of lecithin content in egg - Google Patents
Improve the feedstuff of lecithin content in egg Download PDFInfo
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- CN106173501A CN106173501A CN201610588863.4A CN201610588863A CN106173501A CN 106173501 A CN106173501 A CN 106173501A CN 201610588863 A CN201610588863 A CN 201610588863A CN 106173501 A CN106173501 A CN 106173501A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention relates to a kind of improve the feedstuff of lecithin content in egg, this feedstuff includes Semen Maydis, bean cake, fish flour, rapeseed meal, cottonseed meal, wheat bran, meat meal tankage, bone meal, calcium hydrogen phosphate, stone powder, eugenic element, Sargassum powder, Sal, compound premix for laying period of layers or includes Egg Production of Laying Hens chicken mixed feed, eugenic plain, Sargassum powder.After feedstuff of the present invention is by chicken food, its egg produced is rich in lecithin, this egg is used for the multiple crowd of cardiovascular and cerebrovascular disease and general population, can reduce the multiple crowd of cardiovascular and cerebrovascular disease and suffer from the risk of cardiovascular and cerebrovascular disease, make general population's skin and hair the most healthy glossy.
Description
Technical field
The invention belongs to field of fodder, improve the feedstuff of lecithin in egg particularly to a kind of.
Background technology
Lecithin refers to the general name of various phospholipid, including phosphatidylcholine (phosphatidylchline, PC), phosphatidyl
Ethanolamine (phosphatidyl ethanolamine, PE), phosphatidylinositols (osphatidylinositol, PI) etc..Ovum phosphorus
Fat has multiple benefit to human body, specifically includes: first, and foster brain protects brain function.The foster brain of lecithin protects brain function and mainly shows
At lecithin to arteriocerebral protective effect, this protective effect is because lecithin and has stronger emulsification and oils and fats
Decomposition, can also remove endovascular peroxide simultaneously.In addition, lecithin also helps disappearing of atherosis speckle
Dissipate.Second, it is provided that the exogenous choline of needed by human body, improve brain vigor.This is owing to the activity of human brain be unable to do without neurocyte, and
The signal transmission of neurocyte is main using acetylcholine as mediator.Acetylcholine is synthesized by acetyl and choline.Human body every day is all
Substantial amounts of acetyl, then the choline synthesis of acetyl choline provided with lecithin can be provided.As can be seen here, lecithin can improve note
Recall power and level of intelligence has scientific basis.The choline of needed by human body has 90 percent both to be from lecithin.3rd, protect
Skin cosmetology.Lecithin, as a kind of natural cosmetic material, has suppression alopecia, poliosis blackening, skin care, light speckle wrinkle removing
The effect of stricture of vagina.It is primarily due in lecithin the Major Nutrient source that contained inositol is hair, if lacking lecithin, will
Make hair slowly bleach, even come off.In addition, the molecular structure that lecithin is special makes it have good hydrophilic and parent
Oiliness, so that skin keeps gloss beautiful, fine and smooth lubrication.4th, healthy heart.It has been found that lecithin can make in blood plasma
Cholesterol levels reduces.Therefore it can effectively reduce the sickness rate of cholesterol, hyperlipidemia and coronary heart disease.
According to source difference, lecithin is divided into Ovum Gallus domesticus Flavus lecithin and soybean lecithin, and phospholipid fraction contained by both is different.Egg
In Huang, lecithin content is the highest, reaches 8% the 10% of dry gross mass, and remote to the phosphatidylcholine content that human body is useful
Far above soybean phospholipid, reach 70% 80%.Egg is the animal food that people often eat, China's year per capita consumption chicken
Egg, more than 20 kilograms, far surpasses world average level.Therefore if able to develop the egg of a kind of high lecithin, people are edible
Reduce, also by taking in of lecithin high in egg, the danger that cardiovascular and cerebrovascular disease occurs while egg, reach skin protection hair caring
Effect, will have the most positive meaning.
Summary of the invention
It is an object of the invention to provide and a kind of improve the feedstuff of lecithin in egg, after this feedstuff is by chicken food, it is raw
The egg produced is rich in lecithin, and this egg is used for the multiple crowd of cardiovascular and cerebrovascular disease and general population, can reduce cardiovascular and cerebrovascular vessel
The multiple crowd of disease suffers from the risk of cardiovascular and cerebrovascular disease, makes general population's skin and hair the most healthy glossy.
For achieving the above object, first technical scheme of the present invention is,
Improving the feedstuff of lecithin content in egg, the mass parts of each component is: Semen Maydis 500 690 parts；Bean cake 50 290
Part；Fish flour 0 40 parts；Rapeseed meal 0 60 parts；Cottonseed meal 0 60 parts；0 50 parts of wheat bran；Meat meal tankage 0 60 parts；Bone meal 0 30 parts；Phosphorus
Acid hydrogen calcium 0 15 parts；Stone powder 20 90 parts；Eugenic element 0.5 4 parts；Sargassum powder 5 50 parts；Sal 03 parts；Laying cycle of laying hens is combined
Premix material 5 100 parts.
Preferably technical scheme is that the mass parts of each component of this feedstuff is: Semen Maydis 620 parts, bean cake 190 parts, 49 parts of wheat bran,
Stone powder 80 parts, compound premix for laying period of layers 50 parts, eugenic element 1 part, Sargassum powder 10 parts.
Another preferable technical scheme is, this feedstuff each constituent mass part is: Semen Maydis 605 parts, bean cake 120 parts, Semen Allii Tuberosi
The dregs of rice 40 parts, cottonseed meal 40 parts, fish flour 30 parts, 24 parts of wheat bran, stone powder 70 parts, compound premix for laying period of layers 50 parts, eugenic element 1
Part, Sargassum powder 20 parts.
Second technical scheme of the present invention is
Improve the feedstuff of lecithin content in egg, improve the feedstuff of lecithin content, the mass parts of each component in egg
For: Egg Production of Laying Hens chicken mixed feed 946 994.5 parts, eugenic element 0.5 4 parts, Sargassum powder 5 50 parts.
Preferably technical scheme is that the mass parts of each component is: Egg Production of Laying Hens chicken mixed feed 978 parts, eugenic element 2 parts,
Sargassum powder 20 parts.
Another preferable technical scheme is, the mass parts of each component is: Egg Production of Laying Hens chicken mixed feed 986 parts, eugenic
Element 4 parts, Sargassum powder 10 parts.
Described lecithin uses eugenic element and Sargassum powder to obtain as additive, and lecithin includes phosphatidylcholine, phospholipid
Acyl ethanolamine, phosphatidylinositols.
Compared with prior art, the invention have the benefit that the present invention passes through interpolation in feedstuff eugenic plain and extra large
Algae powder, as the additive of raising lecithin, obtains the present invention and improves the egg feedstuff of lecithin in egg, and this feedstuff is for laying hen
Search for food.Applicant passes through verification experimental verification, and the egg produced after 7 days is rich in lecithin egg, and in this egg, lecithin contains
Amount improves 15% 30%.
The present invention can be effectively increased lecithin content, improves the nutritional labeling of egg, is of value to eater healthy, special
It is not suitable for Patients with Cardiovascular/Cerebrovascular Diseases to eat.
Mass fraction of the present invention be gram or kilogram.
Described compound premix for laying period of layers uses commercially available prod, and described product need to meet condition described in table 1:
Table 1 compound premix for laying period of layers index:
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, to the present invention
Further describe.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not used to limit this
Invention.
The preparation of material
1. eugenic element: purchased from winning development in science and technology company limited of Chongqing City.
2. Sargassum powder: purchased from Binzhou high official position feed corporation,Ltd.
3. Semen Maydis, bean cake, fish flour, rapeseed meal, cottonseed meal, wheat bran, meat meal tankage, bone meal, calcium hydrogen phosphate, stone powder, Sal, egg
Chicken in egg-laying period compound premix all uses commercially available prod.
4. the present embodiment compound feed for laying hen: provided by the honest company limited in Chongqing, item number: 324.
5. the present embodiment compound premix for laying period of layers: purchased from great Bei agriculture group, commercial disignation 865 50.
Embodiment 1
Matched group daily ration composition (mass parts): Semen Maydis 630 parts, bean cake 190 parts, 50 parts of wheat bran, stone powder 80 parts, Egg Production of Laying Hens
Phase compound premix 50 parts.
Test group daily ration (in raising egg of the present invention, the feedstuff of lecithin content, lower same) composition (mass parts): beautiful
620 parts of rice, bean cake 190 parts, 49 parts of wheat bran, stone powder 80 parts, compound premix for laying period of layers 50 parts, eugenic element 1 part, Sargassum powder
10 parts.
Above-mentioned each component fully mixes, and respectively obtains matched group daily ration and test group daily ration.Matched group uses matched group day
Grain is fed, and test group uses test group daily ration to feed:
1. test:
Choose the Roman powder laying hen 144 that 60 week old are healthy, body weight is close, laying rate is close, be divided into matched group and examination
Test group (quantity of two groups of chickens is identical).Use on semi open model hen house, in upper, under, lower four layers of full ladder raise in cages, 9, every cage
Chicken.Being fed by special messenger, free choice feeding, drinking-water, gravity-flow ventilation, every day, light application time was 16h.Artificial lighting, force ventilation, every day
Clear excrement, periodically sterilizes, observes chicken group's health status every day.Conscientiously carrying out every record, immune programme for children is carried out according to a conventional method.
2. process and test period
Matched group and test group, each process 8 repetition, 9 laying hens of each repetition.Experimental period is 28 days, and the phase of raising is 7 in advance
My god, formal test 21 days.
3. lecithin content measures
Within 8th day, the 19th day, the 28th day, take egg and measure lecithin content in egg.Each each process takes 16 pieces of eggs
(2 pieces/repeat), matched group and test group take 96 pieces of eggs for three times altogether.
Assay method:
(1) lecithin extraction:
Take Fresh Egg, remove Ovum Gallus domesticus album, egg yolk is stirred, take 4 5g in 100mL centrifuge tube, add 90mL
95% ethanol solution, is sufficiently stirred for, and 4 000rpm/min are centrifuged 10min, is collected in by ethanol solution in 100mL volumetric flask, with
Dehydrated alcohol is settled to 100mL.
(2) treatments of the sample:
Accurately absorption 10mL above-mentioned egg yolk ethanol solution is in kjeldahl flask, and ethanol is evaporated by heating.Add after cooling
3mL sulphuric acid, 3mL perchloric acid nitric acid digestion liquid, be placed on electric furnace digestion.It is brownish black at the beginning of liquid in Ping, treats that solution becomes colorless
Or during micro-strip clear yellow liquid, i.e. digestion is completely.After solution cools down, add 20mL water, cooling, be transferred to 100mL capacity
In Ping, repeatedly washing kjeldahl flask with water, washing liquid merges to be poured in volumetric flask, is settled to 100mL.This solution is Specimen Determination
Liquid.Take the sulphuric acid commensurability with digesting sample, perchloric acid nitric acid digestion liquid, digest as stated above, as blank solution.
(3) standard regressive method is formulated:
Accurately draw phosphorus titer 0,1.0,2.0,4.0,6.0,8.0,10.0mL, be respectively placed in 20mL tool plug test tube,
Add 10mL vanadium ammonium molybdate developer, shake up, add water to 20mL (i.e. phosphorus concentration be respectively 0,2.5,5.0,10.0,15.0,
20.0,25.0 μ g/mL), mixing, stand 10～15min under 25 DEG C of environment, with water as reference, use 1cm cuvette, at visible point
The absorbance of each solution is surveyed under light photometer 400nm wavelength.With the absorbance measured, phosphorus content is done standard regressive method.
(4) sample determination:
Accurately pipette samples measures liquid 2mL and commensurability blank solution, is respectively placed in 20mL tool plug test tube, by standard regression
The method of equation measures phosphorus content.Lecithin content is calculated further according to below equation.Lecithin content=[(25 × X × 500)/m
×106] × 100%.In formula, X is sample phosphorus content (μ g) gone out according to regression equation calculation, and m is egg yolk sample quality (g),
500 is diluted sample multiple (100/10 × 100/2), and 25 are converted into the coefficient of phospholipid for phosphorus.
The lecithin content contrast table of the egg of the present embodiment and common egg:
| Group | Feed the 8th day | Feed the 19th day | Feed the 28th day | Meansigma methods |
| Matched group | 4.38% ± 0.72%Aa | 5.44% ± 1.63%Aa | 4.90% ± 0.90%Aa | 4.91% ± 1.22%Aa |
| Test group | 5.77% ± 0.90%Bb | 5.94% ± 1.29%Aa | 6.00% ± 1.24%Ab | 5.90% ± 1.12%Bb |
Note: in table, the same letter of mark represents that difference is not notable (P>0.05), different letter representation significant differences (P<
0.05), lower case represents significant level (P=0.05), and capitalization represents pole significant level (P=0.01).Numerical value in table
The form being " means standard deviation " represents.
Test group, compared with matched group, the most significantly improves the content of lecithin, and average lecithin content improves
20.1%.
Embodiment 2
Matched group daily ration composition (mass parts): Semen Maydis 620 parts, bean cake 120 parts, rapeseed meal 40 parts, cottonseed meal 40 parts, fish flour
30 parts, 30 parts of wheat bran, stone powder 70 parts, compound premix for laying period of layers 50 parts.
Test group daily ration composition (mass parts): Semen Maydis 605 parts, bean cake 120 parts, rapeseed meal 40 parts, cottonseed meal 40 parts, fish flour
30 parts, 24 parts of wheat bran, stone powder 70 parts, compound premix for laying period of layers 50 parts, eugenic element 1 part, Sargassum powder 20 parts.
Above-mentioned each component fully mixes, and respectively obtains lecithin in matched group daily ration and raising egg of the present invention and contains
The test group daily ration of amount.Matched group uses matched group daily ration to feed, and test group uses test group daily ration to feed:
1. test
The Roman powder laying hen 144 that 60 week old are healthy, body weight is close, laying rate is close is chosen in test, is divided into matched group
With test group (quantity of two groups of chickens is identical).Use on semi open model hen house, in upper, under, lower four layers of full ladder raise in cages, every cage
9 chickens.Being fed by special messenger, free choice feeding, drinking-water, gravity-flow ventilation, every day, light application time was 16h.Artificial lighting, force ventilation,
Every day, clear excrement, periodically sterilized, and observed chicken group's health status every day.Conscientiously carrying out every record, immune programme for children enters according to a conventional method
OK.
2. process and test period
Matched group and test group, each process 8 repetition, 9 laying hens of each repetition.Experimental period is 28 days, and the phase of raising is 7 in advance
My god, formal test 21 days.
3. lecithin content measures
Within 8th day, the 19th day, the 28th day, take egg and measure lecithin content in egg.Each each process takes 16 pieces of eggs
(2 pieces/repeat), matched group and test group take 96 pieces of eggs for three times altogether.
Assay method:
(1) lecithin extraction:
Take Fresh Egg, remove Ovum Gallus domesticus album, egg yolk is stirred, take 4 5g in 100mL centrifuge tube, add 90mL
95% ethanol solution, is sufficiently stirred for, and 4 000rpm/min are centrifuged 10min, is collected in by ethanol solution in 100mL volumetric flask, with
Dehydrated alcohol is settled to 100mL.
(2) treatments of the sample:
Accurately absorption 10mL above-mentioned egg yolk ethanol solution is in kjeldahl flask, and ethanol is evaporated by heating.Add after cooling
3mL sulphuric acid, 3mL perchloric acid nitric acid digestion liquid, be placed on electric furnace digestion.It is brownish black at the beginning of liquid in Ping, treats that solution becomes colorless
Or during micro-strip clear yellow liquid, i.e. digestion is completely.After solution cools down, add 20mL water, cooling, be transferred to 100mL capacity
In Ping, repeatedly washing kjeldahl flask with water, washing liquid merges to be poured in volumetric flask, is settled to 100mL.This solution is Specimen Determination
Liquid.Take the sulphuric acid commensurability with digesting sample, perchloric acid nitric acid digestion liquid, digest as stated above, as blank solution.
(3) standard regressive method is formulated:
Accurately draw phosphorus titer 0,1.0,2.0,4.0,6.0,8.0,10.0mL, be respectively placed in 20mL tool plug test tube,
Add 10mL vanadium ammonium molybdate developer, shake up, add water to 20mL (i.e. phosphorus concentration be respectively 0,2.5,5.0,10.0,15.0,
20.0,25.0 μ g/mL), mixing, stand 10～15min under 25 DEG C of environment, with water as reference, use 1cm cuvette, at visible point
The absorbance of each solution is surveyed under light photometer 400nm wavelength.With the absorbance measured, phosphorus content is done standard regressive method.
(4) sample determination:
Accurately pipette samples measures liquid 2mL and commensurability blank solution, is respectively placed in 20mL tool plug test tube, by standard regression
The method of equation measures phosphorus content.Lecithin content is calculated further according to below equation.Lecithin content=[(25 × X × 500)/m
×106] × 100%.In formula, X is sample phosphorus content (μ g) gone out according to regression equation calculation, and m is egg yolk sample quality (g),
500 is diluted sample multiple (100/10 × 100/2), and 25 are converted into the coefficient of phospholipid for phosphorus.
The lecithin content contrast table of the egg of the present embodiment and common egg:
| Group | Feed the 8th day | Feed the 19th day | Feed the 28th day | Meansigma methods |
| Matched group | 4.32% ± 0.79%Aa | 5.45% ± 1.60%Aa | 4.94% ± 0.93%Aa | 4.90% ± 1.20%Aa |
| Test group | 6.00% ± 1.43%Bb | 5.58% ± 1.28%Aa | 6.33% ± 0.81%Bb | 5.97% ± 1.21%Bb |
Note: in table, the same letter of mark represents that difference is not notable (P>0.05), different letter representation significant differences (P<
0.05), lower case represents significant level (P=0.05), and capitalization represents pole significant level (P=0.01).Numerical value in table
The form being " means standard deviation " represents.
Test group, compared with matched group, the most significantly improves the content of lecithin, and average lecithin content improves
21.8%.
Embodiment 3
Daily ration based on the honest company limited in Chongqing compound feed for laying hen (item number: 324) selected by feedstuff.
Improve the feedstuff of lecithin content in egg: in 978 parts of basal diets (mass parts), add 2 parts of eugenic element (matter
Amount part) and 20 parts of Sargassum powder (mass parts).Each component is fully mixed.
Matched group uses basal diet to feed, and test group uses the feedstuff of lecithin content in raising egg to carry out feeding
Support:
1. test
The Roman powder laying hen 144 that 60 week old are healthy, body weight is close, laying rate is close is chosen in test, is divided into matched group
With test group (quantity of two groups of chickens is identical).Use on semi open model hen house, in upper, under, lower four layers of full ladder raise in cages, every cage
9 chickens.Being fed by special messenger, free choice feeding, drinking-water, gravity-flow ventilation, every day, light application time was 16h.Artificial lighting, force ventilation,
Every day, clear excrement, periodically sterilized, and observed chicken group's health status every day.Conscientiously carrying out every record, immune programme for children enters according to a conventional method
OK.
2. process and test period
Matched group and test group, each process 8 repetition, 9 laying hens of each repetition.Experimental period is 28 days, and the phase of raising is 7 in advance
My god, formal test 21 days.Control group fed basal diet, test group adds 0.2% eugenic element+2% Sargassum powder.
3. lecithin content measures
Within 8th day, the 19th day, the 28th day, take egg and measure lecithin content in egg.Each each process takes 16 pieces of eggs
(2 pieces/repeat), matched group and test group take 96 pieces of eggs for three times altogether.
Assay method:
(1) lecithin extraction:
Take Fresh Egg, remove Ovum Gallus domesticus album, egg yolk is stirred, take 4 5g in 100mL centrifuge tube, add 90mL
95% ethanol solution, is sufficiently stirred for, and 4 000rpm/min are centrifuged 10min, is collected in by ethanol solution in 100mL volumetric flask, with
Dehydrated alcohol is settled to 100mL.
(2) treatments of the sample:
Accurately absorption 10mL above-mentioned egg yolk ethanol solution is in kjeldahl flask, and ethanol is evaporated by heating.Add after cooling
3mL sulphuric acid, 3mL perchloric acid nitric acid digestion liquid, be placed on electric furnace digestion.It is brownish black at the beginning of liquid in Ping, treats that solution becomes colorless
Or during micro-strip clear yellow liquid, i.e. digestion is completely.After solution cools down, add 20mL water, cooling, be transferred to 100mL capacity
In Ping, repeatedly washing kjeldahl flask with water, washing liquid merges to be poured in volumetric flask, is settled to 100mL.This solution is Specimen Determination
Liquid.Take the sulphuric acid commensurability with digesting sample, perchloric acid nitric acid digestion liquid, digest as stated above, as blank solution.
(3) standard regressive method is formulated:
Accurately draw phosphorus titer 0,1.0,2.0,4.0,6.0,8.0,10.0mL, be respectively placed in 20mL tool plug test tube,
Add 10mL vanadium ammonium molybdate developer, shake up, add water to 20mL (i.e. phosphorus concentration be respectively 0,2.5,5.0,10.0,15.0,
20.0,25.0 μ g/mL), mixing, stand 10～15min under 25 DEG C of environment, with water as reference, use 1cm cuvette, at visible point
The absorbance of each solution is surveyed under light photometer 400nm wavelength.With the absorbance measured, phosphorus content is done standard regressive method.
(4) sample determination:
Accurately pipette samples measures liquid 2mL and commensurability blank solution, is respectively placed in 20mL tool plug test tube, by standard regression
The method of equation measures phosphorus content.Lecithin content is calculated further according to below equation.Lecithin content=[(25 × X × 500)/m
×106] × 100%.In formula, X is sample phosphorus content (μ g) gone out according to regression equation calculation, and m is egg yolk sample quality (g),
500 is diluted sample multiple (100/10 × 100/2), and 25 are converted into the coefficient of phospholipid for phosphorus.
The lecithin content contrast table of the egg of the present embodiment and common egg:
| Group | Feed the 8th day | Feed the 19th day | Feed the 28th day | Meansigma methods |
| Matched group | 4.36% ± 0.72%Aa | 5.52% ± 1.63%Aa | 4.92% ± 0.90%Aa | 4.94% ± 1.22%Aa |
| Test group | 5.96% ± 1.30%Bb | 6.00% ± 1.21%Aa | 6.14% ± 0.79%Ab | 6.03% ± 1.10%Bb |
Note: in table, the same letter of mark represents that difference is not notable (P>0.05), different letter representation significant differences (P<
0.05), lower case represents significant level (P=0.05), and capitalization represents pole significant level (P=0.01).Numerical value in table
The form being " means standard deviation " represents.
Test group, compared with matched group, the most significantly improves the content of lecithin, and average lecithin content improves
22.1%.
Embodiment 4
Daily ration based on the honest company limited in Chongqing compound feed for laying hen (item number: 324) selected by feedstuff.
Improve the feedstuff of lecithin content in egg: in 986 parts of basal diets (mass parts), add 4 parts of eugenic element (matter
Amount part) and 10 parts of Sargassum powder (mass parts).Each component is fully mixed.
Matched group uses basal diet to feed, and test group uses the feedstuff of lecithin content in raising egg to carry out feeding
Support:
1. test
The Roman powder laying hen 144 that 60 week old are healthy, body weight is close, laying rate is close is chosen in test, is divided into matched group
With test group (quantity of two groups of chickens is identical).Use on semi open model hen house, in upper, under, lower four layers of full ladder raise in cages, every cage
9 chickens.Being fed by special messenger, free choice feeding, drinking-water, gravity-flow ventilation, every day, light application time was 16h.Artificial lighting, force ventilation,
Every day, clear excrement, periodically sterilized, and observed chicken group's health status every day.Conscientiously carrying out every record, immune programme for children enters according to a conventional method
OK.
2. process and test period
Matched group and test group, each process 8 repetition, 9 laying hens of each repetition.Experimental period is 28 days, and the phase of raising is 7 in advance
My god, formal test 21 days.
3. lecithin content measures
Within 8th day, the 19th day, the 28th day, take egg and measure lecithin content in egg.Each each process takes 16 pieces of eggs
(2 pieces/repeat), matched group and test group take 96 pieces of eggs for three times altogether.
Assay method:
(1) lecithin extraction:
Take Fresh Egg, remove Ovum Gallus domesticus album, egg yolk is stirred, take 4 5g in 100mL centrifuge tube, add 90mL
95% ethanol solution, is sufficiently stirred for, and 4 000rpm/min are centrifuged 10min, is collected in by ethanol solution in 100mL volumetric flask, with
Dehydrated alcohol is settled to 100mL.
(2) treatments of the sample:
Accurately absorption 10mL above-mentioned egg yolk ethanol solution is in kjeldahl flask, and ethanol is evaporated by heating.Add after cooling
3mL sulphuric acid, 3mL perchloric acid nitric acid digestion liquid, be placed on electric furnace digestion.It is brownish black at the beginning of liquid in Ping, treats that solution becomes colorless
Or during micro-strip clear yellow liquid, i.e. digestion is completely.After solution cools down, add 20mL water, cooling, be transferred to 100mL capacity
In Ping, repeatedly washing kjeldahl flask with water, washing liquid merges to be poured in volumetric flask, is settled to 100mL.This solution is Specimen Determination
Liquid.Take the sulphuric acid commensurability with digesting sample, perchloric acid nitric acid digestion liquid, digest as stated above, as blank solution.
(3) standard regressive method is formulated:
Accurately draw phosphorus titer 0,1.0,2.0,4.0,6.0,8.0,10.0mL, be respectively placed in 20mL tool plug test tube,
Add 10mL vanadium ammonium molybdate developer, shake up, add water to 20mL (i.e. phosphorus concentration be respectively 0,2.5,5.0,10.0,15.0,
20.0,25.0 μ g/mL), mixing, stand 10～15min under 25 DEG C of environment, with water as reference, use 1cm cuvette, at visible point
The absorbance of each solution is surveyed under light photometer 400nm wavelength.With the absorbance measured, phosphorus content is done standard regressive method.
(4) sample determination:
Accurately pipette samples measures liquid 2mL and commensurability blank solution, is respectively placed in 20mL tool plug test tube, by standard regression
The method of equation measures phosphorus content.Lecithin content is calculated further according to below equation.Lecithin content=[(25 × X × 500)/m
×106] × 100%.In formula, X is sample phosphorus content (μ g) gone out according to regression equation calculation, and m is egg yolk sample quality (g),
500 is diluted sample multiple (100/10 × 100/2), and 25 are converted into the coefficient of phospholipid for phosphorus.
The lecithin content contrast table of the egg of the present embodiment and common egg:
| Group | Feed the 8th day | Feed the 19th day | Feed the 28th day | Meansigma methods |
| Matched group | 4.36% ± 0.72%Aa | 5.52% ± 1.63%Aa | 4.92% ± 0.90%Aa | 4.94% ± 1.22%Aa |
| Test group | 5.20% ± 1.26%Aa | 5.93% ± 0.83%Aa | 7.27% ± 3.65%Bb | 6.14% ± 2.393%Bb |
Note: in table, the same letter of mark represents that difference is not notable (P>0.05), different letter representation significant differences (P<
0.05), lower case represents significant level (P=0.05), and capitalization represents pole significant level (P=0.01).Numerical value in table
The form being " means standard deviation " represents.
Test group, compared with matched group, the most significantly improves the content of lecithin, and average lecithin content improves
24.3%.
Claims (8)
1. one kind is improved the feedstuff of lecithin content in egg, it is characterised in that the mass parts of each component of this feedstuff is:
Semen Maydis 500-690 part；Bean cake 50-290 part；Fish flour 0-40 part；Rapeseed meal 0-60 part；Cottonseed meal 0-60 part；Wheat bran 0-50
Part；Meat meal tankage 0-60 part；Bone meal 0-30 part；Calcium hydrogen phosphate 0-15 part；Stone powder 20-90 part；Eugenic element 0.5-4 part；Sargassum powder 5-
50 parts；Sal 0-3 part；Compound premix for laying period of layers 5-100 part.
Feedstuff the most according to claim 1, it is characterised in that: the mass parts of each component of this feedstuff is: Semen Maydis 620 parts, bean
The dregs of rice 190 parts, 49 parts of wheat bran, stone powder 80 parts, compound premix for laying period of layers 50 parts, eugenic element 1 part, Sargassum powder 10 parts.
Feedstuff the most according to claim 1, it is characterised in that: the mass parts of each component of this feedstuff is: Semen Maydis 605 parts, bean
The dregs of rice 120 parts, rapeseed meal 40 parts, cottonseed meal 40 parts, fish flour 30 parts, 24 parts of wheat bran, stone powder 70 parts, compound premix for laying period of layers
50 parts, eugenic element 1 part, Sargassum powder 20 parts.
4. one kind is improved the feedstuff of lecithin content in egg, it is characterised in that the mass parts of each component is: Egg Production of Laying Hens is joined by chicken
Close feedstuff 946-994.5 part, eugenic element 0.5-4 part, Sargassum powder 5-50 part.
Feedstuff the most according to claim 4, it is characterised in that the mass parts of each component is: Egg Production of Laying Hens chicken mixed feed
978 parts, eugenic element 2 parts, Sargassum powder 20 parts.
Feedstuff the most according to claim 4, it is characterised in that the mass parts of each component is: Egg Production of Laying Hens chicken mixed feed
986 parts, eugenic element 4 parts, Sargassum powder 10 parts.
7. according to the arbitrary described feedstuff of claim 1-6, it is characterised in that: described lecithin uses eugenic element and Sargassum powder to make
Obtain for additive.
8. according to the arbitrary described feedstuff of claim 1-6, it is characterised in that: described lecithin includes phosphatidylcholine, phospholipid
Acyl ethanolamine, phosphatidylinositols.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610588863.4A CN106173501A (en) | 2016-07-25 | 2016-07-25 | Improve the feedstuff of lecithin content in egg |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610588863.4A CN106173501A (en) | 2016-07-25 | 2016-07-25 | Improve the feedstuff of lecithin content in egg |
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| CN106173501A true CN106173501A (en) | 2016-12-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610588863.4A Pending CN106173501A (en) | 2016-07-25 | 2016-07-25 | Improve the feedstuff of lecithin content in egg |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106578635A (en) * | 2016-12-10 | 2017-04-26 | 王秀琴 | Laying hen feed for improving lecithin in eggs and method for producing eggs rich in lecithin |
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| CN105614013A (en) * | 2014-11-26 | 2016-06-01 | 天津君合心科技发展有限公司 | Laying hen feed |
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| CN106578635A (en) * | 2016-12-10 | 2017-04-26 | 王秀琴 | Laying hen feed for improving lecithin in eggs and method for producing eggs rich in lecithin |
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Application publication date: 20161207 |