CN106171968B - The adjusting method of the radiosity of laser irradiation seed - Google Patents
The adjusting method of the radiosity of laser irradiation seed Download PDFInfo
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- CN106171968B CN106171968B CN201610832348.6A CN201610832348A CN106171968B CN 106171968 B CN106171968 B CN 106171968B CN 201610832348 A CN201610832348 A CN 201610832348A CN 106171968 B CN106171968 B CN 106171968B
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- 238000000034 method Methods 0.000 title claims abstract description 20
- 230000005855 radiation Effects 0.000 claims abstract description 25
- 230000003287 optical effect Effects 0.000 claims description 2
- 230000009466 transformation Effects 0.000 claims description 2
- 239000011541 reaction mixture Substances 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 5
- 238000009331 sowing Methods 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 abstract description 2
- 230000003750 conditioning effect Effects 0.000 abstract 1
- 230000035772 mutation Effects 0.000 description 5
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000010287 polarization Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241000220225 Malus Species 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Radiation-Therapy Devices (AREA)
Abstract
The adjusting method of the radiosity of laser irradiation seed of the invention is related to a kind of method of test or processing seed, rhizome or the like before sowing or planting, and the specific operation method is as follows: 1) conditioning instrumentation；2) irradiation seed facula area is sought；3) radiosity is adjusted.The condenser lens is thin lens, and the reflecting mirror is 45 ° of inclinations.The adjusting method of the radiosity of laser irradiation seed of the invention, its used equipment is simple, at low cost, and has the advantages that laser radiation power density is flexibly adjustable, it can satisfy in seed irradiation experiment for the adjustable of laser irradiation dose, improve the efficiency of experiment.
Description
Technical Field
The present invention relates to a method for testing or treating seeds, rhizomes or the like before sowing or planting, and more particularly to a method for adjusting the radiation power density of laser-irradiated seeds.
Background
Compared with the traditional ultraviolet mutation breeding, the laser mutation breeding has the advantages of high efficiency, stability, high selectivity, low reversion rate, high directional mutation rate, light radiation damage, contemporary mutation, no pollution and the like, and the laser can also promote the yield of crops and improve the photosynthetic efficiency of plants. Common lasers used for laser mutation breeding are CO2 and He-Ne lasers. Because an important uncertain factor in the laser seed treatment technology is the dose problem of laser irradiation, in order to effectively perform experiments, the radiation power density of the laser needs to be adjustable, so that different experimental requirements can be met.
Disclosure of Invention
The invention aims to overcome the problems and provides a method for adjusting the radiation power density of a laser irradiation seed.
The invention relates to a method for adjusting the radiation power density of a laser irradiation seed, which comprises the following specific operation methods:
1) adjusting the instrument: adjusting the centers of the He-Ne laser, the focusing lens, the double polarizing film, the beam expander and the reflector to the same height, and measuring the distance between the instruments;
2) obtaining the area of the light spot of the irradiated seed: obtaining the area of the irradiation seed light spot according to the parameters of the He-Ne laser and the data measured in the step 1;
3) adjusting the radiation power density: and adjusting the multiple of the beam expander, adjusting the radiation power density or adjusting the double-polarizing film by changing the spot area of the irradiated seeds, and adjusting the radiation power density by changing the emergent light intensity of the double-polarizing film.
As a further improvement of the invention, the focusing lens is a thin lens.
As a further development of the invention, the mirror is tilted by 45 °.
The method for adjusting the radiation power density of the laser irradiation seeds has the advantages of simple equipment and low cost, has the advantage of flexible and adjustable laser radiation power density, can meet the requirement of adjusting the laser irradiation dose in a seed irradiation experiment, and improves the experiment efficiency.
Drawings
FIG. 1 is a schematic diagram of a method of adjusting the radiation power density of a laser irradiated seed according to the present invention.
Detailed Description
The method for adjusting the radiation power density of the laser irradiation seed of the invention is further explained with reference to the attached drawings:
the method for adjusting the radiation power density of the laser irradiation seeds is characterized by comprising the following specific operation methods:
1) adjusting the instrument: adjusting the centers of a He-Ne laser 1, a focusing lens 2, a double polarizer 3, a beam expander 4 and a reflector 5 to the same height, and measuring the distance between the instruments;
as shown in fig. 1, a gaussian beam emitted by a He-Ne laser is focused by a focusing lens, then passes through a double-polarizing film, is amplified by a beam expander and collimated by a collimator lens in sequence, and is reflected to a seed irradiation area on an operation table by a 45-degree reflector; the double polarizing plates are composed of two coaxial polarizing plates capable of rotating relatively, a first polarizing plate (polarizing plate) is adjacent to the focusing lens, and a second polarizing plate (analyzer plate) is adjacent to the beam expander; after adjusting the instrument, the distance between the He-Ne laser and the focusing lens, and the distance Z between the focusing lens and the first polarizing plate were measured1，
2) Obtaining the area of the light spot of the irradiated seed: and (3) obtaining the area of the irradiation seed light spot according to the parameters of the He-Ne laser and the data measured in the step (1), wherein the formula derivation process is as follows:
according to the prior measurement or the parameters of the He-Ne laser in the product specification, the facula of the Gaussian beam emitted by the laser at the output end of the He-Ne laser and the beam waist radius of the emitted Gaussian beam can be obtained, and according to the characteristics of the Gaussian beam, the section radius of the Gaussian beam is related to the beam waist radius and the distance between the section and the beam waist, and the formula is as follows:
in the above formula (1), ω (Z) is the radius of the cross section at a distance Z from the beam waist of the Gaussian beam, ω0The radius of the beam waist of the Gaussian beam emitted by the laser, Z is the distance between the beam waist of the Gaussian beam and the section, and lambda is the wavelength of the laser; by the formula(1) And the reversibility of the optical path, obtain the distance between the beam waist of the Gaussian beam emitted by the laser and the He-Ne laser;
subtracting the distance between the beam waist of the Gaussian beam emitted by the laser and the He-Ne laser from the distance between the He-Ne laser and the focusing lens measured in the step 1 to obtain the distance Z between the beam waist of the Gaussian beam emitted by the laser and the incident surface of the focusing lens0；
Let the radius of the light spot of the Gaussian beam emitted by the laser on the incident surface of the focusing lens be omega1Is a reaction of Z0Substituting equation (1) yields:
by solving the formula (2), the spot radius omega of the Gaussian beam emitted by the laser on the incident surface of the focusing lens can be obtained1；
According to the characteristics of the Gaussian beam, when the Gaussian beam still exits as the Gaussian beam after passing through the focusing lens, the beam waist radius of the Gaussian beam exiting from the focusing lens is set to be omega2Then, the beam waist radii of the gaussian beams at both sides of the focusing lens satisfy the following transformation relationship:
solving the formula (3), wherein f is the known focal length of the focusing lens, and the beam waist radius omega of the Gaussian beam emitted by the focusing lens can be obtained2；
Then, according to the reversibility of the light path, the spot radius of the Gaussian beam emitted by the focusing lens on the emergent surface of the focusing lens is set to be omega3The distance between the waist of the Gaussian beam emitted by the focusing lens and the emitting surface of the focusing lens is Z2The following formula is derived from formula (1):
because the radius of the cross section of the light beam before and after the two sides of the thin lens is equal according to the Gaussian beam characteristics when the focusing lens is a thin lens, the radius of the light spot of the incident surface of the focusing lens (the radius of the light spot of the Gaussian beam emitted by the laser on the incident surface of the focusing lens) is equal to the radius of the light spot of the emergent surface of the focusing lens (the radius of the light spot of the Gaussian beam emitted by the focusing lens on the emergent surface of the focusing lens), namely omega1＝ω3Substituting the distance into formula (4), and finally obtaining the distance Z between the beam waist of the Gaussian beam emitted by the focusing lens and the emitting surface of the focusing lens2；
Setting the distance Z between the beam waist of the Gaussian beam emitted by the focusing lens and the first polaroid3Then, the following formula is given:
Z3＝Z1-Z2 (5)
solving the formula (5), the distance Z between the beam waist of the Gaussian beam emitted from the focusing lens and the first polaroid can be obtained3；
The radius of a light spot of a Gaussian beam emitted by a focusing lens on a first polaroid is set to be omega4And the omega4And omega2Correlation, substituting into equation (1), yields the following equation:
solving the formula (6), the spot radius omega of the Gaussian beam emitted by the focusing lens on the first polaroid can be obtained4Because the distance between the first polarizing film and the second polarizing film is small, the Gaussian beam is regarded as the spot radius unchanged after passing through the double polarizing films, and as can be known from fig. 1, the area of the irradiation seed spot finally reflected to the operation platform and the spot half of the Gaussian beam emitted by the focusing lens on the first polarizing filmDiameter omega4And the multiple of the beam expander is related, the multiple of the beam expander is set as n, and the area of the light spot of the irradiation seed is as follows:
A＝π(nω4)2 (7)
3) adjusting the radiation power density: and adjusting the multiple of the beam expander, adjusting the radiation power density or adjusting the double-polarizing film by changing the spot area of the irradiated seeds, and adjusting the radiation power density by changing the emergent light intensity of the double-polarizing film.
The radiation power density is inversely proportional to the irradiation seed light spot area, and if the multiple of the beam expanding lens is changed according to the formula (7) under the condition that other conditions are not changed, the irradiation seed light spot area is changed, so that the radiation power density is changed;
moreover, the incident light intensity and the emergent light intensity on the two sides of the double-polarizer satisfy the Malus law:
in the above formula (8), I is the intensity of emergent light, I0The power is proportional to the light intensity, and then according to formula (7), under the condition that other conditions are not changed, the included angle theta between the polarization direction of the first polaroid and the polarization direction of the second polaroid is changed, so that the power is changed by changing the emergent light intensity, and further the radiation power density is changed.
The two methods for changing the radiation power density can be used independently or cooperatively.
Claims (1)
1. The method for adjusting the radiation power density of the laser irradiation seeds is characterized by comprising the following specific operation methods:
1) adjusting the instrument: adjusting the centers of the He-Ne laser, the focusing lens, the double polarizing film, the beam expander and the reflector to the same height, and measuring the distance between the instruments; the Gaussian beam emitted by the He-Ne laser passes through the double polarizing film after being focused by the focusing lens, is amplified by the beam expander and is reflected to a seed irradiation area on the operating platform by the 45-degree reflecting mirror; the double polaroids are composed of two polaroids which are coaxial and rotate relatively, a first polaroid is adjacent to the focusing lens, and a second polaroid is adjacent to the beam expander;
2) obtaining the area of the light spot of the irradiated seed: obtaining the area of the irradiation seed light spot according to the parameters of the He-Ne laser and the data measured in the step (1);
according to the parameters of the He-Ne laser, obtaining the facula of the Gaussian beam emitted by the laser at the output end of the He-Ne laser and the beam waist radius of the emitted Gaussian beam, wherein according to the characteristics of the Gaussian beam, the section radius of the Gaussian beam is related to the beam waist radius and the distance between the section and the beam waist, and the formula is as follows:
（1）
wherein,is a section radius, omega, at a distance Z from the waist of the Gaussian beam0The radius of the beam waist of the Gaussian beam emitted by the laser, Z is the distance between the beam waist of the Gaussian beam and the section, and lambda is the wavelength of the laser; obtaining the distance between the beam waist of the Gaussian beam emitted by the laser and the He-Ne laser through the formula (1) and the reversibility of the optical path;
subtracting the distance between the waist of the Gaussian beam emitted by the laser and the He-Ne laser from the measured distance between the He-Ne laser and the focusing lens to obtain the distance Z between the waist of the Gaussian beam emitted by the laser and the incident surface of the focusing lens0；
The radius of a light spot of a Gaussian beam emitted by the laser on the incident surface of the focusing lens is omega1Is a reaction of Z0Substituting equation (1) yields:
(2)
according to the characteristics of the Gaussian beam, when the Gaussian beam still exits as the Gaussian beam after passing through the focusing lens, the focusing lens exits the Gaussian beamWaist radius of omega2Then, the beam waist radii of the gaussian beams at both sides of the focusing lens satisfy the following transformation relationship:
（3）
in the formula (3), the reaction mixture is,fcalculating the radius omega of the beam waist of the Gaussian beam emitted by the focusing lens for the known focal length of the focusing lens2；
According to the reversibility of the light path, the radius of a light spot of the Gaussian beam emitted by the focusing lens on the emergent surface of the focusing lens is omega3The distance between the waist of the Gaussian beam emitted by the focusing lens and the emitting surface of the focusing lens is Z2The following formula is obtained according to formula (1):
（4）
when the focusing lens is a thin lens, ω1＝ω3Calculating the distance Z between the waist of the Gaussian beam emitted by the focusing lens and the exit surface of the focusing lens2；
Distance Z between beam waist of Gaussian beam emitted by focusing lens and first polaroid3The following formula is given:
Z3＝Z1-Z2 (5)
in the formula (5), Z1Calculating the distance Z between the beam waist of the Gaussian beam emitted by the focusing lens and the first polaroid for the distance between the focusing lens and the first polaroid3；
The radius of a light spot of a Gaussian beam emitted by a focusing lens on a first polaroid is set to be omega4And the omega4And omega2Correlation, substituting into equation (1), yields the following equation:
according to the formula, the exit of the focusing lens can be obtainedLight spot radius omega of Gaussian beam on first polaroid 4 And setting the multiple of the beam expanding lens as n, wherein the area of the light spot of the irradiation seed is as follows:
3) adjusting the radiation power density: and adjusting the multiple of the beam expanding lens, and adjusting the radiation power density by changing the area of the light spot of the irradiation seed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610832348.6A CN106171968B (en) | 2016-09-19 | 2016-09-19 | The adjusting method of the radiosity of laser irradiation seed |
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| CN201610832348.6A CN106171968B (en) | 2016-09-19 | 2016-09-19 | The adjusting method of the radiosity of laser irradiation seed |
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| CN106171968A CN106171968A (en) | 2016-12-07 |
| CN106171968B true CN106171968B (en) | 2019-07-12 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1456044A (en) * | 2002-12-18 | 2003-11-19 | 东北林业大学 | Process for overcoming self-incompatibility of plant by laser |
| EP1568264A1 (en) * | 2004-02-25 | 2005-08-31 | Takii & Company, Limited | Method for improving germination of hard seed by laser beam irradiation and germination improved seed |
| CN202738406U (en) * | 2012-04-22 | 2013-02-20 | 任国祚 | Laser seed treatment instrument |
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2016
- 2016-09-19 CN CN201610832348.6A patent/CN106171968B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1456044A (en) * | 2002-12-18 | 2003-11-19 | 东北林业大学 | Process for overcoming self-incompatibility of plant by laser |
| EP1568264A1 (en) * | 2004-02-25 | 2005-08-31 | Takii & Company, Limited | Method for improving germination of hard seed by laser beam irradiation and germination improved seed |
| CN202738406U (en) * | 2012-04-22 | 2013-02-20 | 任国祚 | Laser seed treatment instrument |
Non-Patent Citations (1)
| Title |
|---|
| 氦-氖激光处理蕃茄种子最适剂量的研究;李玉滨等;《中国激光》;19900401;第17卷(第3期);第190页第3段 |
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