CN106138955A - The application in exhausted premenstrual syndrome medicine is treated in preparation of the Fructus Alpiniae Oxyphyllae total flavones - Google Patents
The application in exhausted premenstrual syndrome medicine is treated in preparation of the Fructus Alpiniae Oxyphyllae total flavones Download PDFInfo
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
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- A61K2236/30—Extraction of the material
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- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
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- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The present invention relates to the application in exhausted premenstrual syndrome medicine is treated in preparation of the Fructus Alpiniae Oxyphyllae total flavones, can effectively solve Fructus Alpiniae Oxyphyllae total flavones as sole active agent application problem in preparing exhausted premenstrual syndrome medicine, this Fructus Alpiniae Oxyphyllae total flavones is that Fructus Alpiniae Oxyphyllae is ground into coarse powder, first uses petroleum ether reflux, extract, defat, Fructus Alpiniae Oxyphyllae medicinal residues volatilize petroleum ether, add soak with ethanol, reflux, extract, filter, decompression recycling ethanol, to without alcohol taste, adds distilled water dispersion, obtains medicinal liquid；AB 8 type macroporous adsorbent resin on medicinal liquid, first uses distilled water eluting, discards water elution liquid；Again with mass concentration 10% ethanol elution, discard eluent；Again with mass concentration 80% ethanol elution, collecting 80% ethanol elution, decompression recycling ethanol is to without alcohol taste, lyophilization, pulverizing, obtain Fructus Alpiniae Oxyphyllae total flavones, preparation method of the present invention is simple, easily operation, production efficiency is high, is effective to preparation and treats the medicine of exhausted premenstrual syndrome.
Description
Technical field
The present invention relates to medicine, a kind of Fructus Alpiniae Oxyphyllae total flavones answering in exhausted premenstrual syndrome medicine is treated in preparation
With.
Background technology
Fructus Alpiniae Oxyphyllae, for the fruit of zingiberaceous plant Fructus Alpiniae Oxyphyllae Alpinia oxyphylla Miq., has and warming spleen and stopping diarrha takes the photograph saliva, warm
Effect of kidney reducing urination controlling nocturnal emission with astringent drugs, is usually used in Deficiency and coldness of spleen and stomach, vomiting, has loose bowels, cold type of pain in abdomen, the many salivas of mouth, enuresis due to deficiency of the kidney, and frequent micturition is lost
Essence, nebulousurine.
There is a series of bodies caused by gonadal hormone fluctuation or minimizing and spirit in premenstrual syndrome absolutely before referring to postmenopausal women
Mental symptoms.Menopause (menopause) is divided into natural menopause and artificial menopause.In natural menopause refers to ovary, follicle physiological is exhausted
Caused menopause；Artificial menopause refers to both sides ovary underwent operative excision or by the menopause caused by radiotherapy.Artificial menopause patient is more
Exhausted premenstrual syndrome easily occurs, and clinical symptoms shows themselves in that menoxenia, often occur such as cardiopalmus, dizziness, have a headache, have a sleepless night, tinnitus etc.
Dysautonomia shape, difficult concentrating, and anxious state of mind are big.Show as exciting irritability, anxious or emotion
Low, depressed, can not the emotional symptoms such as self-contr ol, hypomnesis the most more typically, affects the healthy of patient and raw
Bioplasm amount.
Though the multi-medicament treating exhausted premenstrual syndrome is had existing, but total so far there are no the Fructus Alpiniae Oxyphyllae extracted from Fructus Alpiniae Oxyphyllae
Flavone application in exhausted premenstrual syndrome medicine is treated in preparation.
Summary of the invention
For above-mentioned situation, for overcoming the defect of prior art, it is the most yellow that the purpose of the present invention is just to provide a kind of Fructus Alpiniae Oxyphyllae
Ketone application in exhausted premenstrual syndrome medicine is treated in preparation, can effectively solve Fructus Alpiniae Oxyphyllae total flavones and exist as sole active agent
Prepare the application problem in exhausted premenstrual syndrome medicine.
The technical scheme that the present invention solves is, Fructus Alpiniae Oxyphyllae total flavones is as sole active position (composition) in preparation treatment absolutely
Application in premenstrual syndrome medicine, this Fructus Alpiniae Oxyphyllae total flavones is that Fructus Alpiniae Oxyphyllae is ground into coarse powder, first uses petroleum ether reflux, extract,
Defat, Fructus Alpiniae Oxyphyllae medicinal residues volatilize petroleum ether, add soak with ethanol, reflux, extract, filter, and decompression recycling ethanol, to without alcohol taste, adds steaming
Distilled water is disperseed, and obtains medicinal liquid；AB-8 type macroporous adsorbent resin on medicinal liquid, first uses distilled water eluting, discards water elution liquid；Use quality again
Concentration 10% ethanol elution, discards eluent；Again with mass concentration 80% ethanol elution, collect 80% ethanol elution, decompression
Reclaim ethanol extremely without alcohol taste, lyophilization, pulverize, obtain Fructus Alpiniae Oxyphyllae total flavones.
Fructus Alpiniae Oxyphyllae total flavones prepared by the present invention is treated in exhausted premenstrual syndrome medicine in preparation as sole active agent
Application, abundant raw material, preparation method is simple, easily operates, and production efficiency is high, is effective to preparation and treats exhausted premenstrual syndrome
Medicine, has opened up the new application of Fructus Alpiniae Oxyphyllae, is the innovation on Chinese medicine, has significant economic and social benefit.
Detailed description of the invention
Below in conjunction with concrete condition, the detailed description of the invention of the present invention is elaborated.
The present invention in being embodied as, Fructus Alpiniae Oxyphyllae total flavones as sole active position (composition) preparation treatment menopause before
Application in syndrome medicine, this Fructus Alpiniae Oxyphyllae total flavones is the coarse powder that Fructus Alpiniae Oxyphyllae was ground into 20-30 mesh sieve, first adds coarse powder 10
Petroleum ether reflux, extract, 1h of times weight, defat, Fructus Alpiniae Oxyphyllae medicinal residues volatilize petroleum ether, add coarse powder 10 times of weight, mass concentrations
80% soak with ethanol 0.5h, reflux, extract, 1h, filters, obtains filtrate for the first time；Medicinal residues add coarse powder 10 times of weight, mass concentrations again
80% alcohol reflux 1h, filters, and obtains second time filtrate, merges twice filtrate, and decompression recycling ethanol, to without alcohol taste, adds distillation
Moisture is dissipated to be equivalent to, containing crude drug amount 0.3g/ml, obtain medicinal liquid；AB-8 type macroporous adsorbent resin on medicinal liquid, first steams with 2 times of column volumes
Distilled water eluting, discards water elution liquid；Again with mass concentration 10% ethanol elution of 4 times of column volumes, discard eluent；Again with 6 times
Mass concentration 80% ethanol elution of column volume, collects 80% ethanol elution, and decompression recycling ethanol is to without alcohol taste, freezing dry
Dry, pulverize, obtain Fructus Alpiniae Oxyphyllae total flavones.
The present invention is through test, and Fructus Alpiniae Oxyphyllae total flavones, as sole active agent, is combined before can be effectively used for preparation treatment menopause
The medicine of simulator sickness, relevant testing data is as follows:
1 experiment material
1.1 medicine and reagent
Fructus Alpiniae Oxyphyllae total flavones of the present invention, mass content > 52%.Geng Nian An Capsules is (Radix Rehmanniae, Radix Rehmanniae Preparata, Rhizoma Alismatis, Radix Ophiopogonis, profound
Ginseng, Cortex Moutan, Poria, Concha Margaritifera, Rhizoma Curculiginis, Fructus Schisandrae Chinensis, Magnetitum, Caulis Polygoni Multiflori, Ramulus Uncariae Cum Uncis, Fructus Tritici Levis, Radix Polygoni Multiflori Preparata), nourishing YIN for suppressing the hyperactive YANG,
Relieving restlessness is calmed the nerves；For climacteric hectic fever sweating, vertigo and tinnitus, irritated insomnia.Specification: every dress 0.3g；Usage and dosage: mouth
Clothes, one time 3,3 times on the one), Shanxi Yellow River Traditional Chinese Medicine Co., Ltd..Rat E2 detection kit, R&D company；Injection penicillium sp
Element sodium, Huabei Pharmaceutic Co., Ltd, specification: 4,000,000 units；Formalin (analytical pure), Yantai City's chemical industry in pairs is limited
Company；0.9% sodium chloride injection, Cologne, Henan pharmaceutcal corporation, Ltd；Chloral hydrate, the accurate chemical research of Tianjin recovery
Institute；Ethanol, big disinfectant preparation company limited of Xinxiang City three.
1.3 experimental apparatus
TDZ4-WS table-type low-speed centrifuge, Hunan Xiang Yi Laboratory Instruments development corporation, Ltd.；BIORAD-680 enzyme mark
Instrument, 680Microplate Reader, Bio-Rad Laboratories；BX61 electric microscope, OLYMPUS company of Japan；
Manual liquid transfering device, Thermo Fisher Scientific Inc. of the U.S., model JZ19600；HHS-21-8 type electric-heated thermostatic water bath, on
Medical Equipment Plant of Hai Boxun Industrial Co., Ltd.；BCD-248T type refrigerator, Xin Fei Electrical Appliances Co., Ltd；JM1000 electronic scale, remaining
Yao Jiming weighs calibration equipment company limited；AL204 electronic balance, Mettler-Toledo Instrument (Shanghai) Co., Ltd..
1.4 laboratory animal
Cleaning grade mice, Kunming kind, female, 18-22g, Lukang Medical Co., Ltd., Shandong, the animal quality certification number
37005400000020；Laboratory quality certification SYXK (Henan) 2010-001.
2 experimental techniques
2.1 modeling and administration
Modeling method: take Kunming mouse 70, female, 22～25g, take 60 mices, lumbar injection penta bar ratio at random
Appropriate sodium 50mg/kg anesthesia after abdomen position fix, then from mouse back most end rib, midaxillary line and outside spinal column about 1cm
Infall cropping, cuts skin and dorsal muscles about 0.5～1cm after sterilization, the liparitosis that in the otch visual field, a visible milky is shinny,
Ovary i.e. embeds wherein.With pincet clamp gently liparitosis pull-out otch outside, fractionation of fatty group, the most visible one thin-line-shaped
The irregular ovary in yellowish red color.First fallopian tube under ovary (including fat) fine rule is ligatured during clip, then extract ovary, art
After take advantage of a situation cornua uteri put back in abdominal cavity, suture muscles and skin, extract bilateral ovaries with method.Separately take 10 mices for blank right
According to group, perform an operation by upper method, but do not excise ovary.Careful postoperative is raised, and the prevention of intramuscular injection penicillin is infected, continuous 3d.Hands
The mice that starts after art 5d to perform an operation is by only carrying out rat vagina plate coating checking, and every day 1 time, continuous 5d, to determine that ovary is the completeest
Full excision.Smear presents the animal emotionally reacted and is discarded, and selects 60 castration animals to be completely uniformly divided into 5 groups at random.
It is respectively model group, Geng Nian An Capsules group, large and small dosage Fructus Alpiniae Oxyphyllae total flavones group, singly performs an operation the most ovariectomized for blank
Matched group.
Medication: be dissolved in the distilled water boiled by CMC-Na, makes the solution of 0.5% as solvent, by following
Each medicine suspendible is wherein.Take the large and small dosage of Fructus Alpiniae Oxyphyllae total flavones: 20mg mL-1、10mg·mL-1, by 400mg kg-1、
200mg·kg-1Gavage；Geng Nian An Capsules: 33.75mg mL-1, by 675mg kg-1Gavage (is equivalent to the 15 of quantity
Times) gavage；Model group and blank group give the CMC-Na solution of same volume, and administration volume is 0.2mL/10g, every day 1 time, continuously
3 weeks.
2.2 observation item and detection method
2h (fasting 15 hours) after each group mice last gavage, socket of the eye venous blood sampling, separate serum, use radioimmunology to survey
Determine E in serum2, the content of LH, FSH；Dissect mice, extract thymus, spleen, uterine cancer cell, weigh its weight in wet base and calculate internal organs
Index (organ index=internal organs weight in wet base mg/ rat body weight g)；Uterus is fixed in 10% formalin solution, paraffin embedding,
Section, HE dyes, the tectology change that light Microscopic observation is respectively organized.
The assay method of estradiol
Measuring principle: this experiment uses radioimmunoassay method.First calibration object or sample, label and antibody are pressed journey
Sequence regulation is sequentially added in test tube, makes labelling and non-labeled antigen and finite quantity antibody compete with one another for combining, after question response balance,
Add separating medium, centrifugation, make free antigen separate with antigen antibody complex, measure sedimentary radioactive intensity.With
E2 content increases, and its radioactive intensity reduces the most accordingly.
Calibrating reagent:125I-E2, 1 bottle (red liquid)；
Antibody, 1 bottle (blue liquid)；
Calibration object, 7 bottles (liquid), concentration is 0,10,50,100,250,750,2000pg/mL；
Separating medium, 1 bottle (suspension) is with front shaking up；
Quality controlled serum, 2 bottles (liquid), containing two dosage low, high.
Operation sequence: round bottom polystyrene tube is numbered (by multiple pipe operation), according to the form below procedure operation
Sample-adding mensuration flow sheet unit: μ L
Data process: directly given related parameter, calibration curve and sample concentration with the program that γ calculating instrument is prepared in advance.
Experimental result
3.1 impacts on climacteric model mice organ index
Fructus Alpiniae Oxyphyllae total flavones, on enclosing Syndrome Model mouse thymus, spleen, the impact of uterus index before menopause, the results are shown in Table
1。
Table 1 Fructus Alpiniae Oxyphyllae total flavones is on enclosing Syndrome Model mouse thymus, spleen, the impact of uterus index before menopause
Note: compare with model group, * P < 0.05, * * P < 0.01
Can be seen that from upper table, with blank group ratio, the thymus index of model group mice, index and spleen index and uterus index are equal
Significantly reduce (P < 0.01), illustrate that extracing ovary causes climacteric model mice thymus, spleen and the atrophy in uterus.With model
Group ratio, Fructus Alpiniae Oxyphyllae total flavones large and small dosage group and Geng Nian An Capsules group be all remarkably improved model mice thymus index (P <
0.01), being remarkably improved model mice index and spleen index (P < 0.01), Fructus Alpiniae Oxyphyllae total flavones heavy dose group and Geng Nian An Capsules group are equal
It is remarkably improved model mice uterus index (P < 0.01).
3.2 impacts on climacteric model mice E2 level
Fructus Alpiniae Oxyphyllae total flavones is to enclosing Syndrome Model mice serum E before menopause2The impact of level, the results are shown in Table 2.
Table 2 Fructus Alpiniae Oxyphyllae total flavones is to enclosing Syndrome Model mice serum E before menopause2The impact of level
Note: compare with model group, * P < 0.05, * * P < 0.01
Can be seen that from upper table, with blank group ratio, in model group mice serum, E2 level is remarkably decreased (P < 0.01), says
Bright excision ovary causes the sex hormone level in climacteric model mice serum disorderly, and climacteric mouse model is copied into
Merit.With model group ratio, Fructus Alpiniae Oxyphyllae total flavones large and small dosage group and Geng Nian An Capsules group are all remarkably improved model mice serum E2
Level (P < 0.01).
3.3 impacts on climacteric model mice uterus histomorphology
Naive mice Uterine mucosa layer, muscle layer, placenta percreta are normal, and epithelial cell in mucous layer, mucous glands are the most normal.Mould
Type group Mouse Uterus mucous layer, muscle layer, the obvious atrophy of placenta percreta, in mucous layer, all there is atrophy and subtract in epithelial cell, mucous glands
Few.Geng Nian An Capsules group Mouse Uterus mucous layer, muscle layer, placenta percreta obtain a certain degree of recovery, epithelial cell in mucous layer,
Mucous glands is the most necessarily recovered.Fructus Alpiniae Oxyphyllae total flavones heavy dose group Mouse Uterus mucous layer, muscle layer and placenta percreta are the most obvious to be obtained
To recovering, in mucous layer, epithelial cell, mucous glands are substantially restored.Fructus Alpiniae Oxyphyllae total flavones small dose group Mouse Uterus mucosa
Layer, muscle layer and placenta percreta slightly recover, and in mucous layer, epithelial cell, mucous glands slightly recover.
Use micrometer to measure endometrium and the widest part of muscle layer thickness and the narrowest place, seek mean, with method of counting, meter
Calculate body of gland quantity in inner membrance, seek mean, survey the results are shown in Table 3.
Table 3 Fructus Alpiniae Oxyphyllae total flavones is to enclosing the shadow of body of gland in Syndrome Model endometrium of mouse before menopause, muscle layer and inner membrance
Ring
Note: compare with model group, * P < 0.05, * * P < 0.01
Can be seen that from upper table, with blank group ratio, model group endometrium of mouse thickness, muscle layer thickness substantially reduce (P
< 0.01), body of gland number significantly reduces (P < 0.01), illustrates that mice makes metratrophia after climacteric model, and body of gland reduces.With
Model group ratio, Fructus Alpiniae Oxyphyllae total flavones heavy dose group and Geng Nian An Capsules group all can dramatically increase model mice endometrium thickness (P
< 0.01), small dose group can substantially increase model mice endometrium thickness (P < 0.05)；Fructus Alpiniae Oxyphyllae total flavones heavy dose group and
Geng Nian An Capsules group all can dramatically increase model mice myometrium thickness (P < 0.01), can dramatically increase model mice intrauterine
Body of gland quantity (P < 0.01) in film.
Application this product makes the medicine (capsule) for treating exhausted premenstrual syndrome, and the most exhausted through 58 examples of clinical definite
Premenstrual syndrome patient clinical is applied, and achieves significant therapeutic effect.Patient daily 3 times, each 2-3 grain, every contains
Fructus Alpiniae Oxyphyllae total flavones 0.5g, every day 2 times, monthly 1 course for the treatment of, is used in conjunction 2 courses for the treatment of.In addition to 1 people, clinical symptom disappearance or clinical condition
Shape has clear improvement or improver 57 people, and effective percentage is up to 98.3%.Getting well of effect was not expected, be treatment menopause before combine
Innovation on simulator sickness medicine, has opened up the new application of Fructus Alpiniae Oxyphyllae, and economic and social benefit is notable.
Claims (1)
1. Fructus Alpiniae Oxyphyllae total flavones is preparing the application treated in exhausted premenstrual syndrome medicine, this benefit as sole active position
Intelligence core total flavones is the coarse powder that Fructus Alpiniae Oxyphyllae was ground into 20-30 mesh sieve, first adds the petroleum ether reflux, extract, of 10 times of weight of coarse powder
1h, defat, Fructus Alpiniae Oxyphyllae medicinal residues volatilize petroleum ether, add 10 times of weight of coarse powder, mass concentration 80% soak with ethanol 0.5h, reflux, extract,
1h, filters, and obtains filtrate for the first time；Medicinal residues add 10 times of weight of coarse powder, mass concentration 80% alcohol reflux 1h again, filter, and obtain the
Secondary filtrate, merges twice filtrate, and decompression recycling ethanol, to without alcohol taste, adds distilled water and is dispersed to be equivalent to containing crude drug amount 0.3g/
Ml, obtains medicinal liquid；AB-8 type macroporous adsorbent resin on medicinal liquid, first with 2 times of column volume distilled water eluting, discards water elution liquid；Again with 4
Mass concentration 10% ethanol elution of times column volume, discards eluent；Again with mass concentration 80% ethanol elution of 6 times of column volumes,
Collecting 80% ethanol elution, decompression recycling ethanol, to without alcohol taste, lyophilization, is pulverized, is obtained Fructus Alpiniae Oxyphyllae total flavones.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112007121A (en) * | 2020-09-16 | 2020-12-01 | 长春中医药大学附属医院(吉林省中医院) | Oral granule for treating menopause syndrome and preparation process thereof |
| CN113416608A (en) * | 2021-06-29 | 2021-09-21 | 中国热带农业科学院热带作物品种资源研究所 | Extraction method of intelligence-developing essential oil for inhibiting listeria monocytogenes and intelligence-developing essential oil |
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| CN104083473A (en) * | 2014-07-24 | 2014-10-08 | 河南中医学院 | Application of raspberry extract in preparation of drug for treating perimenopausal syndrome |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112007121A (en) * | 2020-09-16 | 2020-12-01 | 长春中医药大学附属医院(吉林省中医院) | Oral granule for treating menopause syndrome and preparation process thereof |
| CN113416608A (en) * | 2021-06-29 | 2021-09-21 | 中国热带农业科学院热带作物品种资源研究所 | Extraction method of intelligence-developing essential oil for inhibiting listeria monocytogenes and intelligence-developing essential oil |
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Application publication date: 20161123 |