CN106135678A - A kind of feed additive and its preparation method and application - Google Patents
A kind of feed additive and its preparation method and application Download PDFInfo
- Publication number
- CN106135678A CN106135678A CN201610687445.0A CN201610687445A CN106135678A CN 106135678 A CN106135678 A CN 106135678A CN 201610687445 A CN201610687445 A CN 201610687445A CN 106135678 A CN106135678 A CN 106135678A
- Authority
- CN
- China
- Prior art keywords
- feed additive
- microcapsule
- biologically active
- chitosan
- plant extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
The invention belongs to feed additive field, be specifically related to a kind of feed additive and its preparation method and application.Feed additive of the present invention is made up of the raw material of preparing of following percentage by weight meter: Chinese mugwort end 30～40%, salt alga powder 20～35%, plant extract 15～20% and biologically active peptide microcapsule 15～25%, wherein the raw material of preparing of plant extract is: Origanum majorana, Cortex Cinnamomi and Fructus Mume, biologically active peptide microcapsule by capsule-core and capsule material with (0.2～0.6): the weight ratio of 1 forms, described capsule-core by phosphopeptide caseinate and antibacterial peptide with (1～4): the weight ratio of (0.5～2) forms, described capsule material by chitosan and sodium alginate with 1:(4～6) weight ratio form.Feed additive of the present invention can promote growth of animal, improve efficiency of feed utilization, strengthens animal immunizing power, reduces disease infection rate, and preventing and treating livestock and poultry class, steady quality, can preserve for a long time, be not susceptible to go mouldy effectively.
Description
Technical field
The invention belongs to feed additive field, be specifically related to a kind of feed additive and its preparation method and application.
Background technology
Along with developing rapidly of China's livestock breeding industry, people to the demand of animal product from incipient quantity
Demand has turned to quality, the demand of safety.In livestock products, harmful substance residual is the subject matter affecting livestock product safety,
Feed additive is to cause one of harmful substance principal element of exceeding standard of residual.
Feed additive as the important raw material of livestock breeding industry, to the production performance of animal, strengthens resistance against diseases, raising
Food utilization efficiency, improve animal product quality and increase economic efficiency etc. and play an important role.But, currently raise
Feed additives also exists more problem in using, first, the easy mildew of feed additive, rotten, and can not effectively preserve
Its bioactive ingredients contained, the especially composition such as microbial ecological agent, feeding enzyme；Secondly, some feed additive is animal
Body absorption is poor, especially with the additive of bioactive ingredients, is lost work by after pepsin hydrolysis the most in vivo
Property；Finally, feed additive often excess, over range, use chemicals over time limit, cause the pathogenic bacteria of drug-resistant in animal body
Constantly occurring with variation pathogen, animal immunizing power reduces, and easily catches an illness and the state of an illness is complicated and changeable, and breeding environment deteriorates, finally
Cause a series of food-safety problem.
Chinese mugwort end removes what the materials such as the mesophyll of fine hair, petiole, vein obtained after crushed during preparing Folium Artemisiae Argyi for Folium Artemisiae Argyi
Powder, Chinese mugwort end has the medical value that Folium Artemisiae Argyi is general, has that warming the meridian for stopping bleeding, dispersing cold for relieving pain, antitussive and antiasthmatic, leak-stopping be antiabortive and dampness
Effect such as antipruritic, although Folium Artemisiae Argyi is widely used in additive for farm animal feed, but about Chinese mugwort end in additive for farm animal feed
Application relevant report less.Fully the using value at exploitation Chinese mugwort end, is advantageously implemented the one-object-many-purposes of Folium Artemisiae Argyi, is prepared by Folium Artemisiae Argyi
During produce Chinese mugwort end turn waste into wealth.
Chinese patent application 201410246729.7 discloses a kind of feed additive and its preparation method and application, described
Feed additive be made up of the raw material of following parts by weight: 40～50 parts of Ampelopsis grossedentata, 40～50 parts of Folium Pini, 10～20 parts auxiliary
Material, wherein said adjuvant be ammonium dihydrogen phosphate and Chinese medicine slag with (1～3): the weight part ratio composition of (10～13), raising of gained
Feed additives has antioxidation, bacteriostasis and sterilization, enhancing immunity, improves production performance, effect of promotion poultry fast-growth, and shows
Write and reduce solid-liquid ratio, there is preferable economic benefit.
Summary of the invention
For solving technical problem present in prior art, it is an object of the invention to provide a kind of feed additive and
Preparation method and application, described feed additive can promote growth of animal, improve efficiency of feed utilization, strengthens animal immunizing power, fall
Low disease infection rate, preventing and treating livestock and poultry class, and steady quality effectively, can preserve for a long time, be not susceptible to go mouldy.
The present invention is by following technical solution to be attained in that
A kind of feed additive is made up of the raw material of preparing of following percentage by weight meter: Chinese mugwort end 30～40%, salt alga powder 20
～35%, plant extract 15～20% and biologically active peptide microcapsule 15～25%.
Preferably, described feed additive is made up of the raw material of preparing of following percentage by weight meter: Chinese mugwort end 40%, salt alga powder
25%, plant extract 15% and biologically active peptide microcapsule 20%.
Further, described plant extract is made up of the raw material of preparing of following percentage by weight meter: Origanum majorana 40～
50%, Cortex Cinnamomi 25～35% and Fructus Mume 20～30%.
Preferably, described plant extract is made up of the raw material of preparing of following percentage by weight meter: Origanum majorana 45%, Cortex Cinnamomi
30% and Fructus Mume 25%.
Further, described biologically active peptide microcapsule by capsule-core and capsule material with (0.2～0.6): the weight ratio of 1 forms, institute
State capsule-core by phosphopeptide caseinate and antibacterial peptide with (1～4): the weight ratio of (0.5～2) forms, described capsule material by chitosan and
Sodium alginate is with 1:(4～6) weight ratio composition.
Preferably, described biologically active peptide microcapsule is made up of with the weight ratio of 0.45:1 with capsule material capsule-core, described capsule-core by
Phosphopeptide caseinate and antibacterial peptide form with the weight ratio of 3:1, described capsule material by chitosan and sodium alginate with the weight ratio of 1:5
Composition.
Further, the preparation method of described biologically active peptide microcapsule comprises the following steps:
S1: take chitosan, is the ratio of 1:4 in solid-liquid ratio, adds the acetic acid that volumetric concentration is 1% molten in chitosan
Liquid, after soaking swelling 60min, 45 DEG C of heating for dissolving, add distilled water, make the chitosan solution that mass body volume concentrations is 1%；
S2: take sodium alginate soluble in water, stirring extremely dissolving, make the sodium alginate soln that mass body volume concentrations is 1%,
Then adding phosphopeptide caseinate, antibacterial peptide in sodium alginate soln, stirring, to being completely dissolved, obtains solution A, standby；
S3: solution A be slowly dropped into in the chitosan solution of 1%, is diluted with water to 2 times under 600rpm stirring by 10 DEG C
Volume, regulates pH to 5～6, and 10 DEG C, 600rpm stirring is lower coagulates reaction 20～30min again, obtains mixed liquor, adds in mixed liquor
Enter its volume 0.1～0.2 times of mass body volume concentrations is the glutaraldehyde solution of 10%, react 1h at 50 DEG C, obtain microcapsule suspension, will
Microcapsule suspension vacuum decompression is dried, and crosses 120 mesh sieves, obtains microcapsule.
Further, the preparation method of described plant extract comprises the following steps:
S1: take Origanum majorana, Cortex Cinnamomi crushed after being dried, adds Fructus Mume, adds water soaking 1h in the ratio that solid-liquid ratio is 1:10,
Reflux, extract, 2 times, each 3h, filters and retains filtering residue, merging filtrate, obtain Aqueous extracts；
S2: adding the ethanol that volume fraction is 60% of filtering residue gross weight 8 times amount in the filtering residue in step S1, backflow carries
Take 2 times, each 2h, filter, merging filtrate, obtain alcohol extract；
S3: Aqueous extracts and alcohol extract are merged, drying under reduced pressure, pulverize, cross 80 mesh sieves, obtain plant extract.
Additionally, the present invention also provides for the preparation method of a kind of described feed additive, comprise the following steps:
Taking Chinese mugwort end, salt alga powder, plant extract mixing, pulverize, cross 100 mesh sieves, add biologically active peptide microcapsule, stirring is all
Even, obtain feed additive, wherein plant extract and biologically active peptide microcapsule are prepared from by above-mentioned preparation method.
Feed additive of the present invention is for preparing the application of animal feed, and described animal feed can be application
In the feedstuff of poultry, such as chicken, duck, goose etc.；Can also be the feedstuff being applied to domestic animal, such as pig, cattle, sheep etc..
Further, the consumption of described feed additive is 3-6kg/t feedstuff.
Compared with prior art, present invention have an advantage that
(1) feed additive of the present invention has preferable bacteriostasis antibiosis, enhancing immunity effect, can replace adding in feedstuff
The chemicals added, reduces chemicals residual, and can be effectively reduced Animal diseases infection rate, effectively prevent and treat livestock and poultry
Class.
(2) raw materials of feed additives of the present invention is easy to get, and safety is good, can promote growth of animal, improve food conversion ratio
And production performance, reduce the cost of livestock and poultry breeding industry, increase economic efficiency.
(3) the feed additive stable in properties of the present invention, can keep the activity of biologically active peptide effectively, gives full play to raw
The effect antibacterial, sorbefacient of thing bioactive peptide, and can preserve for a long time, it is not susceptible to go mouldy.
(4) feed additive of the present invention is using Chinese mugwort end as primary raw material, makes full use of generation in Folium Artemisiae Argyi production process
Residue, it is achieved the cyclic utilization of waste, has important environment protection significance.
Detailed description of the invention
Further describe the present invention below by way of detailed description of the invention, but the present invention is not limited only to following example.
Prepared by embodiment 1 biologically active peptide microcapsule
Biologically active peptide microcapsule of the present invention is made up of the raw material prepared of following group respectively:
A group preparation method:
S1: take 1g chitosan, is the ratio of 1:4 in solid-liquid ratio, adds the acetic acid that volumetric concentration is 1% molten in chitosan
Liquid, after soaking swelling 60min, 45 DEG C of heating for dissolving, add distilled water, make the chitosan solution that mass body volume concentrations is 1%；
S2: take 4g sodium alginate soluble in water, stirring, to dissolving, makes the sodium alginate that mass body volume concentrations is 1% molten
Liquid, then adds phosphopeptide caseinate 0.5g, antibacterial peptide 0.5g in sodium alginate soln, and stirring, to being completely dissolved, obtains solution
A, standby；
S3: solution A be slowly dropped into in the chitosan solution of 1%, is diluted with water to 2 times under 600rpm stirring by 10 DEG C
Volume, regulates pH to 5.5, and 10 DEG C, 600rpm stirring is lower coagulates reaction 25min again, obtains mixed liquor, adds its body in mixed liquor
Long-pending 0.1 times of mass body volume concentrations is the glutaraldehyde solution of 10%, reacts 1h, obtain microcapsule suspension, by microcapsule suspension at 50 DEG C
Vacuum decompression is dried, and crosses 120 mesh sieves, obtains microcapsule.
The preparation method of B-E group microcapsule is with reference to A group.
Embodiment 2 biologically active peptide microcapsule quality testing
The microcapsule preparing A-E group in the embodiment of the present invention 1 carries out mode of appearance, mean diameter, drug loading and envelop rate
Measuring, result see table.
Table 1 present invention strengthens the microcapsule quality measurements of danshensu intestinal absorption
| Group | Microcapsule form | Mean diameter (μm) | Drug loading (%) | Envelop rate (%) |
| A | Single capsule, smooth surface | 93.52 | 62.25 | 94.02 |
| B | Single capsule, smooth surface | 91.76 | 64.82 | 95.87 |
| C | Single capsule, smooth surface | 92.84 | 62.56 | 92.95 |
| D | Single capsule, smooth surface | 93.35 | 62.67 | 93.24 |
| E | Single capsule, smooth surface | 92.47 | 62.83 | 93.69 |
Result shows, the microcapsule encystation that B group prepares is high, smooth surface, and mean diameter is the narrowest, drug loading and envelop rate
The highest, show the microcapsule better quality of B group, therefore, prepare in raw material as feed additive of the present invention using the microcapsule that B group prepares
Biologically active peptide microcapsule.
The preparation of embodiment 3 feed additive
The feed additive of the embodiment of the present invention 3 is made up of the raw material of preparing of following percentage by weight meter:
Chinese mugwort end 40%, salt alga powder 25%, plant extract 15% and biologically active peptide microcapsule 20%, wherein said plant carries
Take thing to be made up of the raw material of preparing of following percentage by weight meter: Origanum majorana 45%, Cortex Cinnamomi 30% and Fructus Mume 25%.
Preparation method:
(1) prepared by plant extract:
S1: take Origanum majorana, Cortex Cinnamomi crushed after being dried, adds Fructus Mume, adds water soaking 1h in the ratio that solid-liquid ratio is 1:10,
Reflux, extract, 2 times, each 3h, filters and retains filtering residue, merging filtrate, obtain Aqueous extracts；
S2: adding the ethanol that volume fraction is 60% of filtering residue gross weight 8 times amount in the filtering residue in step S1, backflow carries
Take 2 times, each 2h, filter, merging filtrate, obtain alcohol extract；
S3: Aqueous extracts and alcohol extract are merged, drying under reduced pressure, pulverize, cross 80 mesh sieves, obtain plant extract；
(2) prepared by feed additive:
Taking Chinese mugwort end, salt alga powder, plant extract mixing, pulverize, cross 100 mesh sieves, add biologically active peptide microcapsule, stirring is all
Even, obtain feed additive.
The preparation of embodiment 4 feed additive
The feed additive of the embodiment of the present invention 4 is made up of the raw material of preparing of following percentage by weight meter:
Chinese mugwort end 30%, salt alga powder 25%, plant extract 20% and biologically active peptide microcapsule 25%, wherein said plant carries
Take thing to be made up of the raw material of preparing of following percentage by weight meter: Origanum majorana 50%, Cortex Cinnamomi 25% and Fructus Mume 25%.
Preparation method is with embodiment 3.
The preparation of embodiment 5 feed additive
The feed additive of the embodiment of the present invention 5 is made up of the raw material of preparing of following percentage by weight meter:
Chinese mugwort end 40%, salt alga powder 30%, plant extract 15% and biologically active peptide microcapsule 15%, wherein said plant carries
Take thing to be made up of the raw material of preparing of following percentage by weight meter: Origanum majorana 40%, Cortex Cinnamomi 35% and Fructus Mume 25%.
Preparation method is with embodiment 3.
The preparation of comparative example 1 feed additive
The raw material of preparing of comparative example 1 feed additive of the present invention forms substantially the same manner as Example 3 with percentage ratio, distinguishes and exists
In, described biologically active peptide is not made microcapsule and is added directly in feed additive, particularly as follows:
Chinese mugwort end 40%, salt alga powder 25%, plant extract 15% and biologically active peptide 20%, wherein said plant extract
It is made up of the raw material of preparing of following percentage by weight meter: Origanum majorana 45%, Cortex Cinnamomi 30% and Fructus Mume 25%, described biologically active peptide
It is made up of with the weight ratio of 3:1 phosphopeptide caseinate and antibacterial peptide.
Preparation method:
(1) prepared by plant extract:
S1: take Origanum majorana, Cortex Cinnamomi crushed after being dried, adds Fructus Mume, adds water soaking 1h in the ratio that solid-liquid ratio is 1:10,
Reflux, extract, 2 times, each 3h, filters and retains filtering residue, merging filtrate, obtain Aqueous extracts；
S2: adding the ethanol that volume fraction is 60% of filtering residue gross weight 8 times amount in the filtering residue in step S1, backflow carries
Take 2 times, each 2h, filter, merging filtrate, obtain alcohol extract；
S3: Aqueous extracts and alcohol extract are merged, drying under reduced pressure, pulverize, cross 80 mesh sieves, obtain plant extract；
(2) prepared by feed additive:
Take Chinese mugwort end, salt alga powder, plant extract mixing, pulverize, cross 100 mesh sieves, add biologically active peptide, stir,
Obtain feed additive.
The preparation of comparative example 2 feed additive
Comparative example 2 feed additive of the present invention to prepare raw material substantially the same manner as Example 3, difference is, without planting
Thing extract, and increase the amount of other each components accordingly, it is specially
Chinese mugwort end 40%, salt alga powder 35% and biologically active peptide microcapsule 25%, wherein said plant extract is by following weight
The raw material of preparing of percentages forms: Origanum majorana 45%, Cortex Cinnamomi 30% and Fructus Mume 25%.
Preparation method:
Take Chinese mugwort end, salt alga powder mixing, pulverize, cross 100 mesh sieves, add biologically active peptide microcapsule, stir, obtain feedstuff
Additive.
Test example one, the mould proof test of feed additive of the present invention
Take the feed additive 200g that embodiment of the present invention 3-5 and comparative example 1-2 prepare respectively, after packing,
Being placed in relative humidity is 75%, and temperature is in the constant-temperature moisture-keeping case of 30 ± 1 DEG C, and routine observation colony growth situation is mould to occur
Become bacterium colony and be judged to the mildew-resistant holding time, and according to GB/T13092-2006 standard test total number of molds, result see table:
The mould proof test result of table 2 feed additive of the present invention
| Group | Mold colony time/d occurs | Total number of molds (cfu/g) |
| Embodiment 3 | 18 | 1.7×103 |
| Embodiment 4 | 18 | 1.8×103 |
| Embodiment 5 | 18 | 1.8×103 |
| Comparative example 1 | 6 | 3.8×105 |
| Comparative example 2 | 9 | 2.0×105 |
Result shows, the feed additive that embodiment of the present invention 3-5 prepares growth under the conditions of accelerated test, to mycete
Having certain inhibition, can extend the time that mycete occurs, and significantly reduce total number of molds, effect is better than comparative example 1 He
Comparative example 2.
Test example two, feed additive of the present invention are on pig growth performance and the impact of immunologic function
1. test method
Screening 60 body weight is about 10kg, healthy piglet, and is numbered, and is then randomly divided into 6 groups, is respectively
Blank group, embodiment 3-5 group and comparative example 1-2 group, often group 10.The piglet feed of blank group is without additive
Feedstuff, the piglet of embodiment 3-5 group takes food the feedstuff of feed additive prepared containing embodiment 3-5 respectively, comparative example 1-2 group
Piglet take food respectively the feedstuff of feed additive prepared containing comparative example 1-2.The freest diet of all pigs, drinking-water, continuously
Observe 2 weeks, record piglet feed consumption during testing and body weight evolution, during calculating feed-weight ratio, and viewing test
The diarrhea rate of piglet.After off-test, extraction piglet blood measures the change of related immune protein level.
2. result of the test
The impact on piglet growth performance of table 3 feed additive of the present invention
Note: compare with starting weight,**P ＜ 0.01；Compare with blank group,#P ＜ 0.05；Compare with comparative example 1 group,△P ＜
0.05；Compare with comparative example 2 groups,■P ＜ 0.05.
The impact on piglet immunological function of table 4 feed additive of the present invention
| Group | Albumin (g/L) | IgG(g/L) | IgM(g/L) | IgA(g/L) |
| Blank group | 39.25±2.46 | 4.05±0.78 | 0.53±0.09 | 0.72±0.06 |
| Embodiment 3 | 43.38±2.40#△■ | 5.16±0.80#△■ | 0.67±0.08#△■ | 0.85±0.08#△■ |
| Embodiment 4 | 43.35±2.37#△■ | 5.13±0.77#△■ | 0.65±0.09#△■ | 0.84±0.07#△■ |
| Embodiment 5 | 43.32±2.43#△■ | 5.10±0.78#△■ | 0.64±0.07#△■ | 0.84±0.06#△■ |
| Comparative example 1 | 40.27±2.25 | 4.32±0.65 | 0.56±0.05 | 0.75±0.07 |
| Comparative example 2 | 40.22±2.16 | 4.28±0.69 | 0.54±0.07 | 0.74±0.08 |
Note: compare with blank group,#P ＜ 0.05；Compare with comparative example 1 group,△P ＜ 0.05；With 2 groups of ratios of comparative example
Relatively,■P ＜ 0.05.
Result shows, after piglet eats the feedstuff 2 weeks of the feed additive prepared containing embodiment of the present invention 3-5, and its body weight
Compare with before test and dramatically increase (P ＜ 0.01)；Comparing with blank group, the feedstuff that embodiment of the present invention 3-5 prepares adds
Agent can dramatically increase the end weight of piglet, average daily gain (P ＜ 0.05), reduces feed-weight ratio (P ＜ 0.05) and diarrhea rate；With contrast
Example 1,2 compares, and the feed additive that embodiment of the present invention 3-5 prepares increases the end weight of piglet, average daily gain, reduces feed-weight ratio
It is respectively provided with significant difference (P ＜ 0.05), shows that feed additive of the present invention can significantly improve the production performance of piglet, promote son
The growth of pig.
Additionally, compare with blank group, the feed additive that embodiment of the present invention 3-5 prepares can dramatically increase piglet blood
Albumin in Qing, immunoglobulin IgG, the level (P ＜ 0.05) of IgM and IgA, its effect also compares with comparative example 1,2 groups
There is significant difference (P ＜ 0.05), show that feed additive of the present invention can be obviously enhanced the immunity of piglet, improve piglet and support
The ability of anti-disease.
Test example three, the feed additive of the present invention impact on chicken resistance against diseases
1. test method
Screen 300 healthy Guangdong Qingyuan's common chicken of 28 ages in days, be then randomly divided into 5 groups, respectively model group, the positive
Matched group, embodiment 3 groups and comparative example 1-2 group, often group 60.Model group feeds basal diet, does not do other and processes, positive right
Adding 50mg/kg chlortetracycline according to group at basal diet, embodiment 3 groups adds, at basal diet, the feedstuff that 0.2% embodiment 3 prepares
Additive, comparative example 1-2 group adds the feed additive that 0.2% comparative example 1,2 prepares, test chicken employing respectively at basal diet
Foster feeding manner, free choice feeding and drinking-water are put down in ground, often organize and take 30 chickens after 2 weeks, 0.5ml/ large intestine bar of lumbar injection
Bacterium solution, remaining 30 chickens, 0.5ml/ Salmonella of lumbar injection, observe 7 and record the incidence of chicken and dead feelings
Condition.
Wherein, morbidity criterion: spirit is depressed, feather is in disorder, sleeping ground, do not drink water, do not eat food；
Dead criterion: after morbidity death, cut open inspection, antibacterial culturing is that escherichia coli are positive or Salmonella is positive.
Protective rate=(test number-death toll)/test number × 100%
2. result of the test
Table 5 feed additive of the present invention is on the impact of resistance against diseases after chicken inoculation escherichia coli
| Group | Test number of elements | Morbidity number | Sickness rate (%) | Death toll | Mortality rate (%) | Protective rate (%) |
| Model group | 30 | 30 | 100 | 28 | 93.3 | 0.07 |
| Embodiment 1 group | 30 | 2 | 0.07 | 0 | 0 | 100 |
| Comparative example 1 group | 30 | 18 | 60 | 9 | 30 | 70 |
| Comparative example 2 groups | 30 | 20 | 66.7 | 12 | 40 | 60 |
| Positive controls | 30 | 4 | 13.3 | 1 | 0.03 | 96.7 |
Table 6 feed additive of the present invention is on the impact of resistance against diseases after chicken inoculation Salmonella
| Group | Test number of elements | Morbidity number | Sickness rate (%) | Death toll | Mortality rate (%) | Protective rate (%) |
| Model group | 30 | 30 | 100 | 30 | 100 | 0 |
| Embodiment 1 group | 30 | 3 | 0.1 | 0 | 0 | 100 |
| Comparative example 1 group | 30 | 20 | 66.7 | 11 | 36.7 | 63.3 |
| Comparative example 2 groups | 30 | 22 | 73.3 | 12 | 40 | 60 |
| Positive controls | 30 | 5 | 16.7 | 2 | 0.07 | 93.3 |
Result shows, the feed additive that the embodiment of the present invention 3 prepares can significantly reduce chicken inoculation escherichia coli or sramana
Sickness rate after Salmonella and mortality rate, reach 100% to the protective rate of chicken defence escherichia coli or Salmonella infection, and effect is excellent
In comparative example 1 and comparative example 2 groups and the best with the effectiveness comparison of positive controls, result above shows, the embodiment of the present invention 3
The feed additive prepared can be effectively reduced disease infection rate, prevents and treats livestock and poultry class.
Below it is only the preferred embodiment of the present invention, it is noted that it is right that above-mentioned preferred implementation is not construed as
The restriction of the present invention, protection scope of the present invention should be as the criterion with claim limited range.For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, it is also possible to make some improvements and modifications, these change
Enter and retouch and also should be regarded as protection scope of the present invention.
Claims (10)
1. a feed additive, it is characterised in that described feed additive is prepared raw material group by following percentage by weight meter
Become: Chinese mugwort end 30～40%, salt alga powder 20～35%, plant extract 15～20% and biologically active peptide microcapsule 15～25%.
Feed additive the most according to claim 1, it is characterised in that described feed additive is by following percentage by weight
The raw material of preparing of meter forms: Chinese mugwort end 40%, salt alga powder 25%, plant extract 15% and biologically active peptide microcapsule 20%.
Feed additive the most according to claim 1 and 2, it is characterised in that described plant extract is by following weight hundred
Proportion by subtraction meter prepare raw material composition: Origanum majorana 40～50%, Cortex Cinnamomi 25～35% and Fructus Mume 20～30%.
Feed additive the most according to claim 3, it is characterised in that described plant extract is by following percentage by weight
The raw material of preparing of meter forms: Origanum majorana 45%, Cortex Cinnamomi 30% and Fructus Mume 25%.
Feed additive the most according to claim 1 and 2, it is characterised in that described biologically active peptide microcapsule by capsule-core with
Capsule material is with (0.2～0.6): the weight ratio of 1 composition, described capsule-core by phosphopeptide caseinate and antibacterial peptide with (1～4): (0.5～
2) weight ratio composition, described capsule material by chitosan and sodium alginate with 1:(4～6) weight ratio form.
Feed additive the most according to claim 5, it is characterised in that described biologically active peptide microcapsule is by capsule-core and capsule material
Forming with the weight ratio of 0.45:1, described capsule-core is made up of with the weight ratio of 3:1 phosphopeptide caseinate and antibacterial peptide, described capsule material
It is made up of with the weight ratio of 1:5 chitosan and sodium alginate.
Feed additive the most according to claim 5, it is characterised in that the preparation method bag of described biologically active peptide microcapsule
Include following steps:
S1: take chitosan, is the ratio of 1:4 in solid-liquid ratio, and adding volumetric concentration in chitosan is the acetum of 1%, leaching
After steeping swelling 60min, 45 DEG C of heating for dissolving, add distilled water, make the chitosan solution that mass body volume concentrations is 1%；
S2: take sodium alginate soluble in water, stirring, to dissolving, makes the sodium alginate soln that mass body volume concentrations is 1%, then
Adding phosphopeptide caseinate, antibacterial peptide in sodium alginate soln, stirring, to being completely dissolved, obtains solution A, standby；
S3: solution A be slowly dropped into in the chitosan solution of 1%, is diluted with water to 2 times of volumes under 600rpm stirring by 10 DEG C,
Regulation pH to 5～6,10 DEG C, the lower multiple solidifying reaction 20～30min of 600rpm stirring, obtain mixed liquor, in mixed liquor, add its body
Long-pending 0.1～0.2 times of mass body volume concentrations is the glutaraldehyde solution of 10%, reacts 1h, obtain microcapsule suspension, mixed by microcapsule at 50 DEG C
Suspension vacuum decompression is dried, and crosses 120 mesh sieves, obtains microcapsule.
8. the method for the feed additive prepared as described in claim 1-7 is arbitrary, it is characterised in that include following step
Rapid:
(1) prepared by biologically active peptide microcapsule:
S1: take chitosan, is the ratio of 1:4 in solid-liquid ratio, and adding volumetric concentration in chitosan is the acetum of 1%, leaching
After steeping swelling 60min, 45 DEG C of heating for dissolving, add distilled water, make the chitosan solution that mass body volume concentrations is 1%；
S2: take sodium alginate soluble in water, stirring, to dissolving, makes the sodium alginate soln that mass body volume concentrations is 1%, then
Adding phosphopeptide caseinate, antibacterial peptide in sodium alginate soln, stirring, to being completely dissolved, obtains solution A, standby；
S3: solution A be slowly dropped into in the chitosan solution of 1%, is diluted with water to 2 times of volumes under 600rpm stirring by 10 DEG C,
Regulation pH to 5～6,10 DEG C, the lower multiple solidifying reaction 20～30min of 600rpm stirring, obtain mixed liquor, in mixed liquor, add its body
Long-pending 0.1～0.2 times of mass body volume concentrations is the glutaraldehyde solution of 10%, reacts 1h, obtain microcapsule suspension, mixed by microcapsule at 50 DEG C
Suspension vacuum decompression is dried, and crosses 120 mesh sieves, obtains microcapsule；
(2) prepared by plant extract:
S1: take Origanum majorana, Cortex Cinnamomi crushed after being dried, adds Fructus Mume, adds water soaking 1h, backflow in the ratio that solid-liquid ratio is 1:10
Extract 2 times, each 3h, filter and retain filtering residue, merging filtrate, obtain Aqueous extracts；
S2: add the ethanol that volume fraction is 60% of filtering residue gross weight 8 times amount, reflux, extract, 2 in the filtering residue in step S1
Secondary, each 2h, filters, merging filtrate, obtains alcohol extract；
S3: Aqueous extracts and alcohol extract are merged, drying under reduced pressure, pulverize, cross 80 mesh sieves, obtain plant extract；
(3) prepared by feed additive:
Take Chinese mugwort end, salt alga powder, plant extract mixing, pulverize, cross 100 mesh sieves, add biologically active peptide microcapsule, stir,
Obtain feed additive.
9. according to the arbitrary described feed additive of claim 1-8 for preparing the application of animal feed.
Feed additive the most according to claim 9 is for preparing the application of animal feed, it is characterised in that described in raise
The consumption of feed additives is 3-6kg/t feedstuff.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610687445.0A CN106135678A (en) | 2016-08-18 | 2016-08-18 | A kind of feed additive and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610687445.0A CN106135678A (en) | 2016-08-18 | 2016-08-18 | A kind of feed additive and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106135678A true CN106135678A (en) | 2016-11-23 |
Family
ID=57331101
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610687445.0A Pending CN106135678A (en) | 2016-08-18 | 2016-08-18 | A kind of feed additive and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106135678A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107970228A (en) * | 2017-11-02 | 2018-05-01 | 天津大学 | A kind of preparation method using chitosan-TPP-KGM as the nano-microcapsule of compound wall materials |
| CN112515050A (en) * | 2020-12-10 | 2021-03-19 | 广州市微生物研究所有限公司 | Antibacterial animal growth promoter containing recombinant human lysozyme and application thereof |
| CN114668074A (en) * | 2022-04-18 | 2022-06-28 | 成都蜀星饲料有限公司 | Feed additive and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101366444A (en) * | 2008-08-30 | 2009-02-18 | 山东阜丰生物科技开发有限公司 | Method for production of high protein salt algae feedstuff additive with glutamic acid wastewater |
| CN101773594A (en) * | 2010-01-07 | 2010-07-14 | 东北农业大学 | Composition for treating sheep diarrhea and preparation method thereof |
| CN102824332A (en) * | 2012-09-14 | 2012-12-19 | 东北农业大学 | Preparation method of antimicrobial peptide slow-release microcapsules |
-
2016
- 2016-08-18 CN CN201610687445.0A patent/CN106135678A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101366444A (en) * | 2008-08-30 | 2009-02-18 | 山东阜丰生物科技开发有限公司 | Method for production of high protein salt algae feedstuff additive with glutamic acid wastewater |
| CN101773594A (en) * | 2010-01-07 | 2010-07-14 | 东北农业大学 | Composition for treating sheep diarrhea and preparation method thereof |
| CN102824332A (en) * | 2012-09-14 | 2012-12-19 | 东北农业大学 | Preparation method of antimicrobial peptide slow-release microcapsules |
Non-Patent Citations (2)
| Title |
|---|
| 梅全喜: "《艾叶的研究与引用》", 31 October 2013 * |
| 祝衰菊等: "中草药饲料抗菌保健剂的研究与应用", 《畜牧业》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107970228A (en) * | 2017-11-02 | 2018-05-01 | 天津大学 | A kind of preparation method using chitosan-TPP-KGM as the nano-microcapsule of compound wall materials |
| CN112515050A (en) * | 2020-12-10 | 2021-03-19 | 广州市微生物研究所有限公司 | Antibacterial animal growth promoter containing recombinant human lysozyme and application thereof |
| CN112515050B (en) * | 2020-12-10 | 2024-04-12 | 广州市微生物研究所集团股份有限公司 | Antibacterial animal growth promoter containing recombinant human lysozyme and application thereof |
| CN114668074A (en) * | 2022-04-18 | 2022-06-28 | 成都蜀星饲料有限公司 | Feed additive and preparation method thereof |
| CN114668074B (en) * | 2022-04-18 | 2024-01-26 | 成都蜀星饲料有限公司 | Feed additive and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101664421B (en) | Traditional Chinese biological preparation for preventing and treating livestock diarrhea and preparation method and feedstuff thereof | |
| CN104161181B (en) | The feed additive of a kind of anti-pig bacterial disease, preparation method and application | |
| CN105410365B (en) | A kind of feed addictive of alternative antibiotic and its application | |
| CN103394053B (en) | Traditional Chinese medicine composition capable of preventing and treating piglet yellow and white scours as well as preparation and applications thereof | |
| CN104304679B (en) | A kind of B B-complex C, E immune polysaccharide microemulsion preparation and its preparation method and application | |
| CN103981129A (en) | Composite microbial preparation, and preparation method and application thereof | |
| CN106135678A (en) | A kind of feed additive and its preparation method and application | |
| CN104435069B (en) | It is a kind of improve pig immune function animal medicinal composition, using and preparation method thereof | |
| CN101715893B (en) | Pure natural piglet immunological enhancement feed additive and application thereof | |
| CN106135705A (en) | A kind of health pig feed additive and preparation and application thereof | |
| CN102304182B (en) | Preparation method and use of enterotoxigenic Escherichia coli egg yolk antibody powder | |
| CN111000033A (en) | Bacillus subtilis viable bacteria fermented traditional Chinese medicine preparation containing thyme oil and traditional Chinese medicine, preparation process and application | |
| CN107348222A (en) | A kind of preparation method of livestock culture feed | |
| CN104161191A (en) | Feed additive for plant oligosaccharide and preparation method thereof | |
| CN103404709B (en) | Feed additive produced from waste liquid and dregs in pharmaceutical factory and preparation method of feed additive | |
| CN106071303A (en) | A kind of egg feedstuff containing lactic acid bacteria | |
| CN110664896A (en) | Anti-stress sudden death prevention compound fermentation traditional Chinese medicine additive and preparation method and application thereof | |
| CN115211492B (en) | Stress-resistant growth-promoting stress-relieving medicine for livestock and poultry, and preparation method and application thereof | |
| CN104161207B (en) | The feed additive of a kind of anti-duck bacterial disease, preparation method and application | |
| CN108936025A (en) | A kind of boar microemulsion formulation and preparation method thereof | |
| KR102334386B1 (en) | Manufacturing method of functional feed for odor reduction | |
| CN103892048A (en) | Feed additive with functions of soothing nerves, enhancing immunity and promoting growth for piglet and preparation method and application of additive | |
| CN107889952A (en) | A kind of fattening pannage additive and its preparation method and application | |
| CN107509871A (en) | A kind of efficiently somatotrophic feedstuff for live pigs and preparation method thereof | |
| CN104757311B (en) | A kind of fish feed additive and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161123 |
|
| RJ01 | Rejection of invention patent application after publication |