CN106135632A - A kind of method of normal temperature degreasing preparation white odourless silkworm chrysalis protein - Google Patents
A kind of method of normal temperature degreasing preparation white odourless silkworm chrysalis protein Download PDFInfo
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- CN106135632A CN106135632A CN201610469290.3A CN201610469290A CN106135632A CN 106135632 A CN106135632 A CN 106135632A CN 201610469290 A CN201610469290 A CN 201610469290A CN 106135632 A CN106135632 A CN 106135632A
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000005238 degreasing Methods 0.000 title claims abstract description 14
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 10
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 10
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 241000382353 Pupa Species 0.000 claims abstract description 42
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 15
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 14
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 13
- 230000001877 deodorizing effect Effects 0.000 claims abstract description 12
- 238000000227 grinding Methods 0.000 claims abstract description 8
- 239000002253 acid Substances 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims description 24
- 239000000843 powder Substances 0.000 claims description 24
- 239000013049 sediment Substances 0.000 claims description 24
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 238000003756 stirring Methods 0.000 claims description 18
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 12
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 12
- 238000005119 centrifugation Methods 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 230000002255 enzymatic effect Effects 0.000 claims description 12
- 239000000706 filtrate Substances 0.000 claims description 12
- 239000000725 suspension Substances 0.000 claims description 12
- 238000002137 ultrasound extraction Methods 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 11
- 102000004882 Lipase Human genes 0.000 claims description 6
- 108090001060 Lipase Proteins 0.000 claims description 6
- 239000004367 Lipase Substances 0.000 claims description 6
- 238000007599 discharging Methods 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 6
- 235000012907 honey Nutrition 0.000 claims description 6
- 239000005457 ice water Substances 0.000 claims description 6
- 238000002386 leaching Methods 0.000 claims description 6
- 235000019421 lipase Nutrition 0.000 claims description 6
- 238000004806 packaging method and process Methods 0.000 claims description 6
- 239000000047 product Substances 0.000 claims description 6
- 238000010298 pulverizing process Methods 0.000 claims description 6
- 239000000376 reactant Substances 0.000 claims description 6
- 238000001291 vacuum drying Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 230000015572 biosynthetic process Effects 0.000 claims description 5
- 241000256844 Apis mellifera Species 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 2
- 239000005864 Sulphur Substances 0.000 claims description 2
- 239000003599 detergent Substances 0.000 claims description 2
- 239000002893 slag Substances 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 230000000050 nutritive effect Effects 0.000 abstract description 6
- 239000002781 deodorant agent Substances 0.000 abstract description 5
- 235000019750 Crude protein Nutrition 0.000 abstract description 3
- 102000004190 Enzymes Human genes 0.000 abstract description 3
- 108090000790 Enzymes Proteins 0.000 abstract description 3
- 238000004458 analytical method Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- CMXPERZAMAQXSF-UHFFFAOYSA-M sodium;1,4-bis(2-ethylhexoxy)-1,4-dioxobutane-2-sulfonate;1,8-dihydroxyanthracene-9,10-dione Chemical compound [Na+].O=C1C2=CC=CC(O)=C2C(=O)C2=C1C=CC=C2O.CCCCC(CC)COC(=O)CC(S([O-])(=O)=O)C(=O)OCC(CC)CCCC CMXPERZAMAQXSF-UHFFFAOYSA-M 0.000 abstract description 3
- 238000003763 carbonization Methods 0.000 abstract description 2
- 238000004090 dissolution Methods 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 239000004094 surface-active agent Substances 0.000 abstract 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 10
- 235000013305 food Nutrition 0.000 description 9
- 150000001413 amino acids Chemical class 0.000 description 8
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 5
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 5
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 5
- 239000011575 calcium Substances 0.000 description 5
- 229910052791 calcium Inorganic materials 0.000 description 5
- 229910052742 iron Inorganic materials 0.000 description 5
- 239000011777 magnesium Substances 0.000 description 5
- 229910052749 magnesium Inorganic materials 0.000 description 5
- 239000011701 zinc Substances 0.000 description 5
- 229910052725 zinc Inorganic materials 0.000 description 5
- 230000003026 anti-oxygenic effect Effects 0.000 description 4
- 239000000470 constituent Substances 0.000 description 4
- 235000013373 food additive Nutrition 0.000 description 4
- 239000002778 food additive Substances 0.000 description 4
- 239000013589 supplement Substances 0.000 description 4
- 238000009413 insulation Methods 0.000 description 3
- HFQQZARZPUDIFP-UHFFFAOYSA-M sodium;2-dodecylbenzenesulfonate Chemical compound [Na+].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O HFQQZARZPUDIFP-UHFFFAOYSA-M 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 238000003916 acid precipitation Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 210000000085 cashmere Anatomy 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000009940 knitting Methods 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- -1 receive Chemical compound 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000009965 tatting Methods 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/02—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from meat
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses the method for a kind of normal temperature degreasing preparation white odourless silkworm chrysalis protein, belong to pupa albumen preparing technical field.The present invention is to have the wormcast of good deodoriging properties and tealeaf residue as raw material, prepare absorbing and deodorizing agent through high temperature carbonization, the normal temperature degreasing under the effect of surfactant and biology enzyme by silkworm chrysalis liquid nitrogen grinding again, obtain crude protein with alkali-soluble acid analysis, integrated treatment through non-polar solven and deodorant after refines, and obtains pupa albumen.The invention has the beneficial effects as follows: preparation process of the present invention is simple, carries out degreasing at normal temperatures, maintains structure and the BA of protein, and the pupa albumen dissolubility after degreasing is good, is of high nutritive value；This pupa albumen dissolution rate reaches more than 94.6%, and yield reaches more than 76.3%, of light color, without obvious bad smell.
Description
Technical field
The present invention relates to the method for a kind of normal temperature degreasing preparation white odourless silkworm chrysalis protein, belong to pupa albumen technology of preparing
Field.
Background technology
Pupa albumen, through the popularization of several years, receives and in many ways pays close attention to, and particularly fiber has smooth such as real silk, soft
The soft feel like cashmere, 18 kinds of amino acid that pupa albumen is contained can take good care of pro-skin, and it is ventilative etc. excellent to resist ultraviolet, moisture absorption
Point, at present in the fields such as the knitting fabric of middle and high end, underwear, socks, tatting shirt, family's textile fabric, filler, non-woven fabrics
Just tool impact.The protein content of silkworm chrysalis more than 50%, the significantly larger than essential amino acid kind in normal food, and protein
Class is complete, and silkworm chrysalis protein is made up of 18 kinds of amino acid, and wherein 8 kinds of amino acid contents of needed by human are very high, are the 2 of pork
Times, 4 times of egg, 10 times of milk, the amino acid nutrient equilibrium of 8 kinds of needed by human, ratio is suitable, meets the United Nations's grain farmer's group
Knit the requirement with the World Health Organization；Silkworm chrysalis also contain potassium, receive, calcium, magnesium, iron, copper, manganese, zinc, phosphorus, selenium and other trace elements, dimension is raw
Element A, E, B1, B2, carrotene etc.；The content of the unrighted acid in silkworm chrysalis is very abundant, accounts for the 72.5% of total fat.Mesh
The extracting method of front pupa albumen: organic solvent extraction, direct acid-precipitation method, alkali-soluble acid analysis method.But owing to pupa albumen is ball
Albumen, water-soluble poor, need in process of production to heat silkworm chrysalis, high temperature extracts for a long time, causes silkworm chrysalis oil to be heated
Oxidation, pupa albumen yield is low and bad smell is dense.
Content of the invention
The technical problem to be solved: be globulin for current pupa albumen, water-soluble poor, conventional method
Extracting pupa albumen all needs at high temperature to carry out degreasing improving its dissolubility, and long high-temp extracting degreasing can destroy egg
The structure of white matter and biologically active, cause the drawback that pupa albumen yield is low and bad smell is dense, provides a kind of good to have
The wormcast of deodoriging properties and tealeaf residue are raw material, prepare absorbing and deodorizing agent through high temperature carbonization, then by silkworm chrysalis liquid nitrogen grinding at table
Under the effect of face activating agent and biology enzyme, normal temperature degreasing, obtains crude protein with alkali-soluble acid analysis, after through non-polar solven and deodorizing
The integrated treatment of agent refines, and obtains the method for pupa albumen.Normal temperature degreasing of the present invention maintains the structure of protein and biology is lived
Property, solving conventional high-temperature degreasing causes the low problem of albumen yield, makes wherein bound fat trip by ultrasonication crude protein
Separate out, under the comprehensive function of non-polar solven and deodorant, remove free-fat, silkworm chrysalis egg in compensate for again in the past preparing
The dense deficiency of white bad smell, is a kind of efficient preparation method.
For solving above-mentioned technical problem, the present invention uses the technical scheme as described below to be:
(1) weigh 400～500g wormcast and 100～200g tealeaf residue mixes, add the honeybee of both gross masses 1/3
Honey, stirring is mixed to form thickness dough, puts into dough in retort, carbonizes under nitrogen protection at 300～400 DEG C
2～3h, discharging, absorbing and deodorizing agent must be made by oneself, standby；
(2) put into 1～2kg fresh silkworm chrysalis in liquid nitrogen grinding machine and pulverize, silkworm chrysalis powder is loaded in enzymatic vessel, simultaneously to
Wherein adding the distilled water of silkworm chrysalis powder volume 3～4 times, stir formation suspension, adds 3～5g detergent alkylate sulphur
Acid sodium and the lipase of suspension gross mass 5～8%, be placed in ultrasonator, ultrasonic mixing 15～20min；
(3) after ultrasonic mixing, enzymatic vessel is put in insulating box, at 35～45 DEG C, be incubated enzymolysis 5～7h, by enzymolysis product
Proceed in centrifuge, with 7000～8000r/min rotating speed centrifugation 10～15min, remove supernatant, obtain lower sediment,
Degreased pupa powder end；
(4) above-mentioned degreased pupa powder end being dispersed in 8～10 times of mass concentrations of its volume is 2～3% sodium hydroxide solutions
In, move in high pressure homogenizer after mixing, process 30～40min with the pressure homogeneous of 3～5MPa, then homogenizing fluid is put into 40～
In 50 DEG C of water-baths, stirring and leaching 20～30min, filters and removes filter residue, obtain filtrate；
(5) be the 1mol/L hydrochloric acid above-mentioned filtrate pH to 4.0～5.0 of regulation by concentration, and put into 4～6 DEG C of ice-water baths stand heavy
Form sediment 2～3h, filters to obtain sediment, is 1:4 by solid-to-liquid ratio, loads in ultrasonic extraction instrument, simultaneously after mixing sediment and acetone
Weigh the standby self-control absorbing and deodorizing agent of the quality such as sediment, put in ultrasonic extraction instrument after gauze parcel, with 300～400W
Power ultrasonic oscillating reactions 40～60min；
(6) after reaction terminates, by reactant horizontal centrifuge centrifugation 10～15min, remove supernatant and obtain filter residue, will filter
Slag is placed in vacuum drying and is dried, and puts into pulverizing in pulverizer, crosses 200 mesh standard sieves, packaging, obtains pupa albumen.
The application process of the present invention: the pupa albumen preparing the present invention adds to food as food additives, adds
Dosage is food gross mass 3～5%, can effectively supplement amino acid, improves the antioxygenic property of human body, moreover it is possible to effectively facilitate enteron aisle
To zinc, iron, magnesium, the absorption of calcium constituent, this pupa albumen dissolubility is good, is of high nutritive value, and is worthy to be popularized and uses.
The present invention is compared with additive method, and Advantageous Effects is:
(1) preparation process of the present invention is simple, carries out degreasing at normal temperatures, maintains structure and the BA of protein, de-
Pupa albumen dissolubility after fat is good, is of high nutritive value；
(2) this pupa albumen dissolution rate reaches more than 94.6%, and yield reaches more than 76.3%, of light color, without obvious bad smell.
Detailed description of the invention
First weigh 400～500g wormcast and 100～200g tealeaf residue mixes, add both gross masses 1/3
Honey, stirring be mixed to form thickness dough, dough is put in retort, under nitrogen protection at 300～400 DEG C
Charing 2～3h, discharging, absorbing and deodorizing agent must be made by oneself, standby；Then 1～2kg fresh silkworm chrysalis is put in liquid nitrogen grinding machine and grind
Cheng Fen, loads silkworm chrysalis powder in enzymatic vessel, is added thereto to the distilled water of silkworm chrysalis powder volume 3～4 times simultaneously, stirs
Form suspension, add 3～5g neopelex and the lipase of suspension gross mass 5～8%, be placed in Under Ultrasonic Vibration
Swing in device, ultrasonic mixing 15～20min；After ultrasonic mixing, enzymatic vessel is put in insulating box, at 35～45 DEG C, be incubated enzyme
Solve 5～7h, enzymolysis product is proceeded in centrifuge, with 7000～8000r/min rotating speed centrifugation 10～15min, in removal
Clear liquid, obtains lower sediment, obtains degreased pupa powder end；Again above-mentioned degreased pupa powder end is dispersed in 8～10 times of matter of its volume
Amount concentration is in 2～3% sodium hydroxide solutions, moves in high pressure homogenizer after mixing, processes 30 with the pressure homogeneous of 3～5MPa
～40min, then homogenizing fluid is put in 40～50 DEG C of water-baths, stirring and leaching 20～30min, filters and removes filter residue, obtain filtrate；
It is the 1mol/L hydrochloric acid above-mentioned filtrate pH to 4.0～5.0 of regulation by concentration, and put in 4～6 DEG C of ice-water baths and staticly settle 2～3h,
Filter to obtain sediment, be 1:4 by solid-to-liquid ratio, load in ultrasonic extraction instrument after sediment and acetone are mixed, weigh precipitation simultaneously
The standby self-control absorbing and deodorizing agent of the quality such as thing, puts in ultrasonic extraction instrument, with 300～400W power ultrasonic after gauze parcel
Oscillating reactions 40～60min；After reaction terminates, by reactant horizontal centrifuge centrifugation 10～15min, remove supernatant
Obtain filter residue, filter residue is placed in vacuum drying and is dried, and put into pulverizing in pulverizer, cross 200 mesh standard sieves, packaging, obtain silkworm
Pupa albumen.
Example 1
First weigh 400g wormcast and 100g tealeaf residue mixes, add the honey of both gross masses 1/3, stirring mixing
Form thickness dough, dough is put in retort, at 300 DEG C, carbonize 2h, discharging under nitrogen protection, suction must be made by oneself
Attached deodorant, standby；Then put into 1kg fresh silkworm chrysalis in liquid nitrogen grinding machine and pulverize, silkworm chrysalis powder is loaded enzymatic vessel
In, it is added thereto to the distilled water of silkworm chrysalis powder volume 3 times simultaneously, stir formation suspension, adds 3g dodecyl
Benzene sulfonic acid sodium salt and the lipase of suspension gross mass 5%, be placed in ultrasonator, ultrasonic mixing 15min；After ultrasonic mixing,
Putting into enzymatic vessel in insulating box, at 35 DEG C, enzymolysis product is proceeded to, in centrifuge, turn with 7000r/min by insulation enzymolysis 5h
Speed centrifugation 10min, removes supernatant, obtains lower sediment, obtains degreased pupa powder end；Again by above-mentioned degreased pupa powder end uniformly
Being scattered in 8 times of mass concentrations of its volume is in 2% sodium hydroxide solution, moves in high pressure homogenizer, with the pressure of 3MPa after mixing
Homogeneous processes 30min, then puts into homogenizing fluid in 40 DEG C of water-baths, stirring and leaching 20min, filters and removes filter residue, obtains filtrate；With
Concentration is the 1mol/L hydrochloric acid above-mentioned filtrate pH to 4.0 of regulation, and puts in 4 DEG C of ice-water baths and staticly settle 2h, filters to obtain sediment,
It is 1:4 by solid-to-liquid ratio, load in ultrasonic extraction instrument after sediment and acetone are mixed, weigh the standby of the quality such as sediment simultaneously
Self-control absorbing and deodorizing agent, puts in ultrasonic extraction instrument, with 300W power ultrasonic oscillating reactions 40min after gauze parcel；Reaction
After end, by reactant horizontal centrifuge centrifugation 10min, remove supernatant and obtain filter residue, filter residue is placed in vacuum drying
Middle drying, and put into pulverizing in pulverizer, cross 200 mesh standard sieves, packaging, obtain pupa albumen.
The pupa albumen preparing the present invention adds to food as food additives, and addition is food gross mass
3%, can effectively supplement amino acid, improve the antioxygenic property of human body, moreover it is possible to effectively facilitate enteron aisle to zinc, iron, magnesium, calcium constituent
Absorbing, this pupa albumen dissolubility is good, is of high nutritive value, and is worthy to be popularized and uses.
Example 2
First weigh 450g wormcast and 150g tealeaf residue mixes, add the honey of both gross masses 1/3, stirring mixing
Form thickness dough, dough is put in retort, at 350 DEG C, carbonize 3h, discharging under nitrogen protection, suction must be made by oneself
Attached deodorant, standby；Then put into 2kg fresh silkworm chrysalis in liquid nitrogen grinding machine and pulverize, silkworm chrysalis powder is loaded enzymatic vessel
In, it is added thereto to the distilled water of silkworm chrysalis powder volume 4 times simultaneously, stir formation suspension, adds 4g dodecyl
Benzene sulfonic acid sodium salt and the lipase of suspension gross mass 7%, be placed in ultrasonator, ultrasonic mixing 18min；After ultrasonic mixing,
Putting into enzymatic vessel in insulating box, at 40 DEG C, enzymolysis product is proceeded to, in centrifuge, turn with 7500r/min by insulation enzymolysis 6h
Speed centrifugation 13min, removes supernatant, obtains lower sediment, obtains degreased pupa powder end；Again by above-mentioned degreased pupa powder end uniformly
Being scattered in 9 times of mass concentrations of its volume is in 3% sodium hydroxide solution, moves in high pressure homogenizer, with the pressure of 4MPa after mixing
Homogeneous processes 35min, then puts into homogenizing fluid in 45 DEG C of water-baths, stirring and leaching 25min, filters and removes filter residue, obtains filtrate；With
Concentration is the 1mol/L hydrochloric acid above-mentioned filtrate pH to 4.5 of regulation, and puts in 5 DEG C of ice-water baths and staticly settle 3h, filters to obtain sediment,
It is 1:4 by solid-to-liquid ratio, load in ultrasonic extraction instrument after sediment and acetone are mixed, weigh the standby of the quality such as sediment simultaneously
Self-control absorbing and deodorizing agent, puts in ultrasonic extraction instrument, with 350W power ultrasonic oscillating reactions 50min after gauze parcel；Reaction
After end, by reactant horizontal centrifuge centrifugation 13min, remove supernatant and obtain filter residue, filter residue is placed in vacuum drying
Middle drying, and put into pulverizing in pulverizer, cross 200 mesh standard sieves, packaging, obtain pupa albumen.
The pupa albumen preparing the present invention adds to food as food additives, and addition is food gross mass
4%, can effectively supplement amino acid, improve the antioxygenic property of human body, moreover it is possible to effectively facilitate enteron aisle to zinc, iron, magnesium, calcium constituent
Absorbing, this pupa albumen dissolubility is good, is of high nutritive value, and is worthy to be popularized and uses.
Example 3
First weigh 500g wormcast and 200g tealeaf residue mixes, add the honey of both gross masses 1/3, stirring mixing
Form thickness dough, dough is put in retort, at 400 DEG C, carbonize 3h, discharging under nitrogen protection, suction must be made by oneself
Attached deodorant, standby；Then put into 2kg fresh silkworm chrysalis in liquid nitrogen grinding machine and pulverize, silkworm chrysalis powder is loaded enzymatic vessel
In, it is added thereto to the distilled water of silkworm chrysalis powder volume 4 times simultaneously, stir formation suspension, adds 5g dodecyl
Benzene sulfonic acid sodium salt and the lipase of suspension gross mass 8%, be placed in ultrasonator, ultrasonic mixing 20min；After ultrasonic mixing,
Putting into enzymatic vessel in insulating box, at 45 DEG C, enzymolysis product is proceeded to, in centrifuge, turn with 8000r/min by insulation enzymolysis 7h
Speed centrifugation 15min, removes supernatant, obtains lower sediment, obtains degreased pupa powder end；Again by above-mentioned degreased pupa powder end uniformly
Being scattered in 10 times of mass concentrations of its volume is in 3% sodium hydroxide solution, moves in high pressure homogenizer, with the pressure of 5MPa after mixing
Power homogeneous processes 40min, then puts into homogenizing fluid in 50 DEG C of water-baths, stirring and leaching 30min, filters and removes filter residue, obtains filtrate；
It is the 1mol/L hydrochloric acid above-mentioned filtrate pH to 5.0 of regulation by concentration, and puts in 6 DEG C of ice-water baths and staticly settle 3h, filter and to obtain precipitation
Thing, is 1:4 by solid-to-liquid ratio, loads in ultrasonic extraction instrument, weigh the quality such as sediment simultaneously after mixing sediment and acetone
Standby self-control absorbing and deodorizing agent, puts in ultrasonic extraction instrument, with 400W power ultrasonic oscillating reactions 60min after gauze parcel；
After reaction terminates, by reactant horizontal centrifuge centrifugation 15min, remove supernatant and obtain filter residue, filter residue is placed in vacuum
Drying is dried, and puts into pulverizing in pulverizer, cross 200 mesh standard sieves, packaging, obtain pupa albumen.
The pupa albumen preparing the present invention adds to food as food additives, and addition is food gross mass
5%, can effectively supplement amino acid, improve the antioxygenic property of human body, moreover it is possible to effectively facilitate enteron aisle to zinc, iron, magnesium, calcium constituent
Absorbing, this pupa albumen dissolubility is good, is of high nutritive value, and is worthy to be popularized and uses.
Claims (1)
1. the method for a normal temperature degreasing preparation white odourless silkworm chrysalis protein, it is characterised in that concrete preparation process is:
(1) weigh 400～500g wormcast and 100～200g tealeaf residue mixes, add the honeybee of both gross masses 1/3
Honey, stirring is mixed to form thickness dough, puts into dough in retort, carbonizes under nitrogen protection at 300～400 DEG C
2～3h, discharging, absorbing and deodorizing agent must be made by oneself, standby；
(2) put into 1～2kg fresh silkworm chrysalis in liquid nitrogen grinding machine and pulverize, silkworm chrysalis powder is loaded in enzymatic vessel, simultaneously to
Wherein adding the distilled water of silkworm chrysalis powder volume 3～4 times, stir formation suspension, adds 3～5g detergent alkylate sulphur
Acid sodium and the lipase of suspension gross mass 5～8%, be placed in ultrasonator, ultrasonic mixing 15～20min；
(3) after ultrasonic mixing, enzymatic vessel is put in insulating box, at 35～45 DEG C, be incubated enzymolysis 5～7h, by enzymolysis product
Proceed in centrifuge, with 7000～8000r/min rotating speed centrifugation 10～15min, remove supernatant, obtain lower sediment,
Degreased pupa powder end；
(4) above-mentioned degreased pupa powder end being dispersed in 8～10 times of mass concentrations of its volume is 2～3% sodium hydroxide solutions
In, move in high pressure homogenizer after mixing, process 30～40min with the pressure homogeneous of 3～5MPa, then homogenizing fluid is put into 40～
In 50 DEG C of water-baths, stirring and leaching 20～30min, filters and removes filter residue, obtain filtrate；
(5) be the 1mol/L hydrochloric acid above-mentioned filtrate pH to 4.0～5.0 of regulation by concentration, and put into 4～6 DEG C of ice-water baths stand heavy
Form sediment 2～3h, filters to obtain sediment, is 1:4 by solid-to-liquid ratio, loads in ultrasonic extraction instrument, simultaneously after mixing sediment and acetone
Weigh the standby self-control absorbing and deodorizing agent of the quality such as sediment, put in ultrasonic extraction instrument after gauze parcel, with 300～400W
Power ultrasonic oscillating reactions 40～60min；
(6) after reaction terminates, by reactant horizontal centrifuge centrifugation 10～15min, remove supernatant and obtain filter residue, will filter
Slag is placed in vacuum drying and is dried, and puts into pulverizing in pulverizer, crosses 200 mesh standard sieves, packaging, obtains pupa albumen.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106616446A (en) * | 2017-01-20 | 2017-05-10 | 西南大学 | Meat product containing silkworm pupa protein and making method of meat product |
Citations (3)
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|---|---|---|---|---|
| CN103288946A (en) * | 2013-05-17 | 2013-09-11 | 广西大学 | Preparation method of white, low-fat and odorless silkworm pupa protein |
| CN104226250A (en) * | 2014-09-26 | 2014-12-24 | 四川农业大学 | Preparation method of heavy metal copper and cadmium biochar adsorbent and product thereof |
| CN104561204A (en) * | 2014-12-10 | 2015-04-29 | 内蒙古天奇生物科技有限公司 | Method for preparing cerebroprotein hydrolysate by using non-solvent degreasing |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103288946A (en) * | 2013-05-17 | 2013-09-11 | 广西大学 | Preparation method of white, low-fat and odorless silkworm pupa protein |
| CN104226250A (en) * | 2014-09-26 | 2014-12-24 | 四川农业大学 | Preparation method of heavy metal copper and cadmium biochar adsorbent and product thereof |
| CN104561204A (en) * | 2014-12-10 | 2015-04-29 | 内蒙古天奇生物科技有限公司 | Method for preparing cerebroprotein hydrolysate by using non-solvent degreasing |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106616446A (en) * | 2017-01-20 | 2017-05-10 | 西南大学 | Meat product containing silkworm pupa protein and making method of meat product |
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