CN106134769A - A kind of manufacture method of Boletus edulis Bull ex Franch original seed - Google Patents
A kind of manufacture method of Boletus edulis Bull ex Franch original seed Download PDFInfo
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- CN106134769A CN106134769A CN201610573329.6A CN201610573329A CN106134769A CN 106134769 A CN106134769 A CN 106134769A CN 201610573329 A CN201610573329 A CN 201610573329A CN 106134769 A CN106134769 A CN 106134769A
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- franch
- boletus edulis
- edulis bull
- bull
- sporophore
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
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Abstract
The invention discloses the manufacture method of a kind of Boletus edulis Bull ex Franch original seed, it is by fresh Folium Pini, peeled potatoes, Boletus edulis Bull ex Franch Producing Area Soil, tryptone, carbonyl diamine, beef extract, vitamin B1, dipotassium hydrogen phosphate, ferrous sulfate, calcium chloride, glucose, chloromycetin, granular active carbon, graininess maturation Boletus edulis Bull ex Franch sporophore, gibberellins etc. make specific Boletus edulis Bull ex Franch pedigree seed culture medium, obtain through medium sterilization, inoculation, spawn culture.The present invention compared with prior art has the most prominent feature, and the manufacture method of Boletus edulis Bull ex Franch original seed of the present invention is simple, and strain is pure；Production cost can be made to reduce by 10 15%；Strain pollution can reduce by more than 25%；Mycelial biomass (136.42mg/kg) improves more than 25mg/kg than traditional method, and the infection intensity (75%) of forest is improved more than 20% than traditional method by strain.
Description
Technical field
The present invention relates to edible and medical fungi strain manufacture technology field, be specifically related to the making side of a kind of Boletus edulis Bull ex Franch original seed
Method.
Background technology
Boletus edulis Bull ex Franch (Boletus edulis) is also known as big foot mushroom, Suillus albidipes (Peck) Sing..Sporophore is medium to large-scale.Cap is flat
Hemispherical or the most open and the most flat, does not glues, smooth, and edge is pure, yellowish-brown, drabon color or russet.Bacterial context white, thick, injured rear constant
Color.Tube initial stage white, after in light color, straight raw or the most twin, or the peripheral recesses at handle.The mouth of pipe is circular, and base portion slightly expands light
Brown or khaki, interior reality.
Boletus edulis Bull ex Franch is grown in the mountain area of 600-1500m, belongs to high temperature modification bacterium, and mycelial growth thermophilic is 23-28 DEG C, typically
Being grown on the Coniferous forest of High aititude and the mixed forest of broad-leaf forest the 7-9 month, mycelial growth requires there is scattered light, the ground of i.e. seven cloudy three sun
Side, early stage arid 7, August the alternate time fruiting of weather many, increment is big.
Boletus edulis Bull ex Franch is the most nutritious, it is most important that after the cooking, abnormal taste is delicious, gets used to eating outside meat
The most delicious, it is the rarest delicious food.With burning fry, then become dish mouthfeel happy, delicious flavour, the Baoshang of use,
Then bacterium fragrance spread four, fragrant strongly fragrant smooth, makes perfect taste, abundant nutrition integrate with medical value.
Boletus edulis Bull ex Franch contains the necessary 8 kinds of aminoacid of abundant polysaccharide, protein and human body, is world-renowned high-quality
Wild edible fungus, especially more praises highly with the Western European countries and likes.Boletus edulis Bull ex Franch have clearing heat and relieving fidgetness, wind-evil dispelling and cold-evil expelling, nourshing blood and promoting blood circulation,
Effect that qi-restoratives is refreshed oneself, has stronger active anticancer and influenza, the effect of preventing cold, can treat lumbago and skelalgia, Shou Zuma
Wood and infertility.
Boletus edulis Bull ex Franch is the treasure in feast, is again the tonic non-defective unit having long enjoyed a good reputation.Boletus edulis Bull ex Franch sporophore band
Having the more fungus of kind and the antibacterial of substantial amounts, owing to being restricted by spawn culture technology, Boletus edulis Bull ex Franch is introduced a fine variety tame and docile
Chemical industry never obtains breakthrough, and its exploitation depend on wild resource more.Boletus edulis Bull ex Franch resource is caused to be opened
Sending out utilization and lie substantially in the state of " growth, freely gathers, spontaneous trade naturally ", every collecting season, the people adopting bacterium are plunderred
Gathering Boletus edulis Bull ex Franch with taking formula by force, seriously compromise Boletus edulis Bull ex Franch and rely in existence, the natural environment of growth, Boletus edulis Bull ex Franch produces
Amount reduces year by year, even faces the condition of extinction in imminent danger in some region.
Summary of the invention
The technical problem to be solved be overcome existing Boletus edulis Bull ex Franch strain pollution rate height, growth cycle length,
The problem of complex process, it is provided that a kind of new Boletus edulis Bull ex Franch original seed manufacture method so that the Boletus edulis Bull ex Franch original seed work made
Skill is easy, strain is pure, pollute less, simple to operate, the cycle is short (6～8d), and cost reduces (10-15%).
Technical scheme is as follows:
The manufacture method of a kind of Boletus edulis Bull ex Franch original seed, the method is to add a little in Boletus edulis Bull ex Franch pedigree seed culture medium
Activated carbon and graininess Boletus edulis Bull ex Franch sporophore, add gibberellins, incubation in shaking table after medium sterilization cools down
In, activated carbon can adsorb a large amount of miscellaneous bacteria, and gibberellins can accelerate Boletus edulis Bull ex Franch cell division, mature cell longitudinal tensile strain, internode
Cell elongation, so that mycelium apposition growth is around graininess Boletus edulis Bull ex Franch sporophore, thus shortens incubation time；Should
Method comprises the steps: culture medium making, medium sterilization, inoculation, spawn culture.
Wherein:
Culture medium makes: calculates by weight, fresh Folium Pini 150-200 part is cleaned, peeled potatoes 150-200 part
It is cut into bulk, Boletus edulis Bull ex Franch Producing Area Soil 100-150 part, fresh Folium Pini, block Rhizoma Solani tuber osi and Producing Area Soil are added 1000 parts
In water, boil 30～40min, filter off fresh Folium Pini, Rhizoma Solani tuber osi residue and undissolvable soil particle with gauze and obtain filtrate, filtrate
Middle addition tryptone 6-10 part, carbonyl diamine 0.52-0.60 part, beef extract 0.45-0.55 part, vitamin
B10.000028-0.000035 part, dipotassium hydrogen phosphate 0.65-0.75 part, ferrous sulfate 0.50-0.60 part, calcium chloride 0.55-
0.65 part, glucose 10-15 part, chloromycetin 0.010-0.015 part, granular active carbon 10-15 part, graininess maturation delicious food Hepar Bovis seu Bubali
Mushroom entity 10-15 part, adds water and is settled to 1000 parts, and control ph is 5.5-6.5.
Medium sterilization is: culture medium be sub-packed in triangular flask, every bottle of 150-200ml, with on tampon lid, at 110-
With the high pressure steam sterilization 25-35min that pressure is 0.1～0.15Mpa at 130 DEG C, after cooling, add gibberellins 0.005-0.008
Part, it being subsequently placed in 24～28 DEG C of constant incubators and cultivate 36-48h, the fluid medium of asepsis growth is retained standby.
Inoculation is: put in superclean bench by sterile growth media and inoculating tool, uviol lamp sterilizing 30-40min
After, Boletus edulis Bull ex Franch sporophore entirety is wiped one time gently, then by alcohol burner with the ethanol of 75% in superclean bench
Lighting, vaccinating lancet puts alcohol burner flame envelope calcination 1-2s, before the Boletus edulis Bull ex Franch sporophore sprouting 1-2d is placed in alcohol burner flame envelope
At the 5-6cm of top, it is divided into 2-3 with the vaccinating lancet after calcination and distinguishes standby, then take the Boletus edulis Bull ex Franch of cap and stem joint
Sporophore tissue 5-10g, puts in the culture medium of asepsis growth gently.
Spawn culture is: the culture medium inoculated is placed in 22-26 DEG C, trains in the spawn culture shaking table of 120-140r/min
Support 6～8d, treat that covering with white hypha in culture medium can carry out next step inoculation cultivation.
The present invention compared with prior art has the most prominent feature, the manufacture method of Boletus edulis Bull ex Franch original seed of the present invention
Simply, strain is pure；Production cost can be made to reduce 10-15%；Strain pollution can reduce by more than 25%；Mycelial biomass
(136.42mg/kg) more than 25mg/kg is improved than traditional method, the strain comparable traditional method of infection intensity (75%) to forest
Improve more than 20%.The method of the present invention can realize the scale of Boletus edulis Bull ex Franch original seed, industrialization produces, and opens one for peasant
Bar road of getting wealth.
Detailed description of the invention
In order to deepen to understand the present invention, below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1:
1, the manufacture method of a kind of Boletus edulis Bull ex Franch original seed, it is characterised in that: comprise the following steps
(1) culture medium makes: calculate by weight, is cleaned by 150 parts of fresh Folium Pini, and peeled potatoes 150 parts is cut into block
Shape, Boletus edulis Bull ex Franch Producing Area Soil 100 parts, fresh Folium Pini, block Rhizoma Solani tuber osi and Producing Area Soil are added in 1000 parts of water, boils 30
～40min, filter off fresh Folium Pini, Rhizoma Solani tuber osi residue and undissolvable soil particle with gauze and obtain filtrate, filtrate adds pancreas egg
White peptone 6 parts, carbonyl diamine 0.52 part, beef extract 0.45 part, vitamin B10.000028 part, dipotassium hydrogen phosphate 0.65 part, sulfur
Ferrous 0.50 part of acid, 0.55 part of calcium chloride, glucose 10 parts, 0.010 part of chloromycetin, granular active carbon 10 parts, graininess is ripe
Boletus edulis Bull ex Franch sporophore 10 parts, adds water and is settled to 1000 parts, and control ph is 5.5-6.5.
(2) medium sterilization: culture medium be sub-packed in triangular flask, every bottle of 150ml, with on tampon lid, at 110-130 DEG C
Lower pressure is the high pressure steam sterilization 25min of 0.1～0.15Mpa, adds gibberellins 0.005 part, be subsequently placed in 24 after cooling
DEG C constant incubator cultivates 36h, and the fluid medium of asepsis growth is retained standby；
(3) inoculation: sterile growth media and inoculating tool are put in superclean bench, after uviol lamp sterilizing 30min,
Boletus edulis Bull ex Franch sporophore entirety is wiped gently one time, then by alcohol burner point by superclean bench with the ethanol of 75%
Combustion, vaccinating lancet is put alcohol burner flame envelope calcination 1s, the Boletus edulis Bull ex Franch sporophore sprouting 1d is placed in alcohol burner flame envelope front upper place 5cm
Place, is divided into 2 with the vaccinating lancet after calcination and distinguishes standby, then take the Boletus edulis Bull ex Franch sporophore tissue of cap and stem joint
5g, puts in the culture medium of asepsis growth gently；
(4) spawn culture: the culture medium inoculated is placed in 26 DEG C, cultivates in the spawn culture shaking table of 120-140r/min
6d, treats that covering with white hypha in culture medium can carry out next step inoculation cultivation.
Embodiment 2:
1, the manufacture method of a kind of Boletus edulis Bull ex Franch original seed, it is characterised in that: comprise the following steps
(1) culture medium makes: calculate by weight, is cleaned by 200 parts of fresh Folium Pini, and peeled potatoes 200 parts is cut into block
Shape, Boletus edulis Bull ex Franch Producing Area Soil 150 parts, fresh Folium Pini, block Rhizoma Solani tuber osi and Producing Area Soil are added in 1000 parts of water, boils
40min, filters off fresh Folium Pini, Rhizoma Solani tuber osi residue and undissolvable soil particle with gauze and obtains filtrate, adds Trypsin in filtrate
Peptone 10 parts, carbonyl diamine 0.60 part, beef extract 0.55 part, vitamin B10.000035 part, dipotassium hydrogen phosphate 0.75 part, sulphuric acid
Ferrous 0.60 part, 0.65 part of calcium chloride, glucose 15 parts, 0.015 part of chloromycetin, granular active carbon 15 parts, graininess maturation is beautiful
Taste bolete sporophore 15 parts, adds water and is settled to 1000 parts, and control ph is 5.5-6.5.
(2) medium sterilization: culture medium be sub-packed in triangular flask, every bottle of 200ml, with on tampon lid, at 110-130 DEG C
Lower pressure is the high pressure steam sterilization 35min of 0.1～0.15Mpa, adds gibberellins 0.008 part, be subsequently placed in 28 after cooling
DEG C constant incubator cultivates 36-48h, and the fluid medium of asepsis growth is retained standby；
(3) inoculation: sterile growth media and inoculating tool are put in superclean bench, after uviol lamp sterilizing 40min,
Boletus edulis Bull ex Franch sporophore entirety is wiped gently one time, then by alcohol burner point by superclean bench with the ethanol of 75%
Combustion, vaccinating lancet is put alcohol burner flame envelope calcination 2s, the Boletus edulis Bull ex Franch sporophore sprouting 2d is placed in alcohol burner flame envelope front upper place 6cm
Place, is divided into 3 with the vaccinating lancet after calcination and distinguishes standby, then take the Boletus edulis Bull ex Franch sporophore tissue of cap and stem joint
10g, puts in the culture medium of asepsis growth gently；
(4) spawn culture: the culture medium inoculated is placed in 26 DEG C, cultivates in the spawn culture shaking table of 120-140r/min
8d, treats that covering with white hypha in culture medium can carry out next step inoculation cultivation.
The above, be only presently preferred embodiments of the present invention, non-the present invention makees any pro forma restriction, although
The present invention is disclosed above with preferred embodiment, but not to limit the present invention, any those skilled in the art,
In the range of technical solution of the present invention, when method and the technology contents of available the disclosure above are made a little change or repair
Decorations are the Equivalent embodiments of equivalent variations, as long as being an off the content of technical solution of the present invention, according to the technical spirit according to invention
Any simple modification, equivalent variations and the modification being made above example, still falls within the range of technical solution of the present invention.
Claims (5)
1. the manufacture method of a Boletus edulis Bull ex Franch original seed, it is characterised in that: the method is at Boletus edulis Bull ex Franch pedigree seed culture medium
The a little activated carbon of middle addition and graininess Boletus edulis Bull ex Franch sporophore, add gibberellins, at shaking table after medium sterilization cools down
In middle incubation, activated carbon can adsorb a large amount of miscellaneous bacteria, and gibberellins can accelerate Boletus edulis Bull ex Franch cell division, mature cell longitudinally
Elongation, internodal cell elongation, so that mycelium apposition growth is around graininess Boletus edulis Bull ex Franch sporophore, thus shorten training
The time of supporting；The method comprises the steps: culture medium making, medium sterilization, inoculation, spawn culture.
The manufacture method of Boletus edulis Bull ex Franch original seed the most according to claim 1, it is characterised in that described culture medium makes and is:
Calculating by weight, fresh Folium Pini 150-200 part cleaned, peeled potatoes 150-200 part is cut into bulk, and Boletus edulis Bull ex Franch produces
Ground soil 100-150 part, adds fresh Folium Pini, block Rhizoma Solani tuber osi and Producing Area Soil in 1000 parts of water, boils 30～40min, use
Gauze filters off fresh Folium Pini, Rhizoma Solani tuber osi residue and undissolvable soil particle and obtains filtrate, adds tryptone 6-10 part in filtrate,
Carbonyl diamine 0.52-0.60 part, beef extract 0.45-0.55 part, vitamin B10.000028-0.000035 part, phosphoric acid hydrogen two
Potassium 0.65-0.75 part, ferrous sulfate 0.50-0.60 part, calcium chloride 0.55-0.65 part, glucose 10-15 part, chloromycetin
0.010-0.015 part, granular active carbon 10-15 part, graininess maturation Boletus edulis Bull ex Franch sporophore 10-15 part, add water and be settled to
1000 parts, control ph is 5.5-6.5.
The manufacture method of Boletus edulis Bull ex Franch original seed the most according to claim 1, it is characterised in that described medium sterilization is:
Culture medium is sub-packed in triangular flask, every bottle of 150-200ml, with on tampon lid, at 110-130 DEG C with pressure be 0.1～
The high pressure steam sterilization 25-35min of 0.15Mpa, adds gibberellins 0.005-0.008 part, is subsequently placed in 24～28 DEG C after cooling
Constant incubator cultivates 36-48h, and the fluid medium of asepsis growth is retained standby.
The manufacture method of Boletus edulis Bull ex Franch original seed the most according to claim 1, it is characterised in that described inoculation is: by aseptic
Growth medium and inoculating tool are put in superclean bench, after uviol lamp sterilizing 30-40min, use in superclean bench
Boletus edulis Bull ex Franch sporophore entirety is wiped one time by the ethanol of 75% gently, is then lighted by alcohol burner, and vaccinating lancet puts alcohol burner
Flame envelope calcination 1-2s, is placed at alcohol burner flame envelope front upper place 5-6cm, after calcination by the Boletus edulis Bull ex Franch sporophore sprouting 1-2d
Vaccinating lancet be divided into 2-3 and distinguish standby, then take the Boletus edulis Bull ex Franch sporophore tissue 5-10g of cap and stem joint, gently
Put in the culture medium of asepsis growth.
The manufacture method of Boletus edulis Bull ex Franch original seed the most according to claim 1, it is characterised in that described spawn culture is: will
The culture medium inoculated is placed in 22-26 DEG C, cultivates 6～8d in the spawn culture shaking table of 120-140r/min, treats in culture medium long
Full white hypha can carry out next step inoculation cultivation.
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| CN201610573329.6A CN106134769A (en) | 2016-07-20 | 2016-07-20 | A kind of manufacture method of Boletus edulis Bull ex Franch original seed |
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| CN201610573329.6A CN106134769A (en) | 2016-07-20 | 2016-07-20 | A kind of manufacture method of Boletus edulis Bull ex Franch original seed |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110506570A (en) * | 2019-08-15 | 2019-11-29 | 贵州大学 | A kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus and method promoting Growth of Masson Pine |
| CN110637680A (en) * | 2018-06-27 | 2020-01-03 | 贵州金蟾大山生物科技有限责任公司 | Production method of Dictyophora rubrovalvata mildew-removing high-germination-rate cultivation stock |
| CN110999722A (en) * | 2020-01-03 | 2020-04-14 | 沈阳农业大学 | Culture medium and culture method of dictyophora phalloidea |
| CN112005811A (en) * | 2020-09-22 | 2020-12-01 | 蒋亚涛 | Edible fungus cultivation material and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110637680A (en) * | 2018-06-27 | 2020-01-03 | 贵州金蟾大山生物科技有限责任公司 | Production method of Dictyophora rubrovalvata mildew-removing high-germination-rate cultivation stock |
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| CN110506570A (en) * | 2019-08-15 | 2019-11-29 | 贵州大学 | A kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus and method promoting Growth of Masson Pine |
| CN110999722A (en) * | 2020-01-03 | 2020-04-14 | 沈阳农业大学 | Culture medium and culture method of dictyophora phalloidea |
| CN112005811A (en) * | 2020-09-22 | 2020-12-01 | 蒋亚涛 | Edible fungus cultivation material and preparation method thereof |
| CN112005811B (en) * | 2020-09-22 | 2022-12-16 | 东宁市林达工贸有限公司 | Edible fungus cultivation material and preparation method thereof |
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