CN106124753B - Electrochemical luminescence buffer solution and cleaning fluid - Google Patents
Electrochemical luminescence buffer solution and cleaning fluid Download PDFInfo
- Publication number
- CN106124753B CN106124753B CN201610538476.XA CN201610538476A CN106124753B CN 106124753 B CN106124753 B CN 106124753B CN 201610538476 A CN201610538476 A CN 201610538476A CN 106124753 B CN106124753 B CN 106124753B
- Authority
- CN
- China
- Prior art keywords
- cleaning fluid
- proclin
- buffer solution
- tpa
- tripropyl amine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
Abstract
The present invention relates to a kind of electrochemical luminescence buffer solution and cleaning fluid suitable for Roche automatic lmunoassays analyzer, greatly reduce the dosage of the larger tripropyl amine (TPA) of toxicity in buffer formulation, and add the material of enhanced chemiluminescence, reduce the toxicity of original-pack reagent, reduce the operational risk of docimaster, preparation of reagents is simple, as a result accurately；The effective cleaning composition increased to compositions such as coagula and fibrins is cleaned in formula of liquid, and adds the preservatives of Proclin 300, further safeguards electrode.Cleaning fluid cleaning performance is good, holding time length.The present invention has extremely strong industrial application value.
Description
Technical field
The present invention relates to the chemiluminescent buffer and cleaning fluid available for Roche automatic lmunoassays analyzer, belongs to medical treatment
Apparatus external diagnosis reagent field.
Background technology
Electrogenerated chemiluminescence (ECL), also referred to as electrochemical luminescence, it is a kind of novel to be that chemiluminescence is combined with electrochemistry
Research method.It is to be electrochemically reacted by applying certain voltage to electrode, certain between product or in product and system
Chemically reacted between kind component, electron transition is produced excitation state material to excitation state, excitation state material returns to ground state production
Hair tonic light；Either luminescent substance provides energy using electrode and directly carries out redox reaction, generates unstable intermediate state
Material, intermediate state material can decompose generation light radiation rapidly.
Roche companies of Switzerland Cobas e411/e601 Electrogenerated chemiluminescent immunoassay systems, it is to apply advanced electrochemistry
The Full-automatic desk type analyzer of luminous (ECL) technology.Thyreoidine, hormone, myocardial damage and tumor-marker, infection are applied
Disease, anaemia, Bone m etabolism etc. widely determine project.Its electrochemiluminescsystem system is tris (bipyridine) ruthenium ([Ru (bpy) 32+])-three
Propylamine (TPrA) system, its matching used Procell and Cleancell dosage is larger, and supporting original-pack reagent is particularly
Procell is expensive and toxicity is larger.
Chemiluminescent buffer Procell is tripropyl amine (TPA) cleaning fluid, for safeguarding electrode, transporting reactant mixture, cleaning
The coated particulate of Streptavidin, produce reaction signal；Chemiluminescence cleaning fluid Cleancell, for pipeline after measuring every time
The cleaning of system and measuring cell and electrodes maintenance.Roche Diagnistics import registration certificate presentation of information Procell is formulated：Phosphate
Buffer solution 300mmol/L, tripropyl amine (TPA) 180mmol/L, Qu Gou Ji≤0.1%；Preservative, pH6.8；Cleancell is formulated：
KOH 176mmol/L (equivalent to pH value 13.2)；Cleaning agent≤1%.Tris (bipyridine) ruthenium ([Ru (bpy) 32+])-tripropyl amine (TPA)
(TPrA) system is a kind of common ECL systems, and wherein tris (bipyridine) ruthenium is electrochemical luminescence agent, and it and electron donor TPrA exist
Electrode anode surface loses electronics and the oxidation reaction, [Ru (bpy) 3 of divalent occurs simultaneously2+] label is oxidized to the [Ru of trivalent
(bpy)33+] label, TPrA is oxidized to radical cation TPrA+*, TPrA+*It is very unstable, spontaneously lose one
Proton and form free radical TPrA*, it is strong reductant, by [Ru (bpy) 3 of the electronics to trivalent3+], become and excite
The Ru (bpy) 3 of state2+, and TPrA itself is oxidized to oxidation product.The Ru (bpy) 3 of excitation state2+Launch a wavelength during decay
620nm photon, re-form the Ru (bpy) 3 of ground state2+.The progress that this process is gone round and begun again in electrode surface, produce many
Photon so that optical signal strengthens.
Because light caused by the irreversibility of TprA autoxidations and interaction between the two is weaker, the system is led to
Often the use of large excess of TPrA (is typically TPrA：Ru(bpy)32+=100000 μm of ol/L：1 μm of ol/L), i.e. 100mmol/L,
And original-pack reagent adds 180mmol/L TPrA, beyond usual amounts, and TPrA toxicity is identified as high toxicity, rat oral LD50
=72mg/kg, its steam or mist have strong impulse to eye, mucous membrane, the upper respiratory tract and skin, for the healthy shadow of docimaster
Sound is larger.In addition, full automatic biochemical apparatus is after a number of uses, because cleaning performance is bad, from measuring cell to pipeline, easily by
The pollution of reaction solution, long-term protein precipitation, causes blank absorbency to increase, and alarms.
It can be seen that chemiluminescent buffer and cleaning fluid of the further research available for Roche automatic lmunoassays analyzer,
The concentration of tripropyl amine (TPA) is especially reduced, and ensures the uniformity of testing result, the buffer solution of stability and accuracy, and have
The cleaning fluid of more excellent cleaning performance, it is still technical problem urgently to be resolved hurrily, this is also exactly what the present invention was accomplished
Leaned on where basic with power.
The content of the invention
As described above, in order to further study available for Roche automatic lmunoassays analyzer chemiluminescent buffer and
Cleaning fluid, especially reduces the concentration of tripropyl amine (TPA), and ensures the uniformity of testing result, the buffer solution of stability and accuracy,
And the cleaning fluid with more excellent cleaning performance, the present inventor conduct in-depth research to this, are paying a large amount of wounds
After the property made work, so as to complete the present invention.
The present invention is with regard to TPrA and Ru (bpy) 32+Interaction caused by light is weaker sets about, Procell formulas are changed
Enter, substantially reduce TPrA dosages, while N is added in prescription, [just] butylaminos of N- bis- ethanol, sodium chloride, PEG 300, third
The materials such as diureide, paracresol, enhanced chemiluminescence, reduce the chemiluminescence caused by tripropyl amine (TPA) concentration reduces and weaken, meanwhile,
Ensure the uniformity and stability with original-pack reagent.
The present invention adds the compositions such as urea, thiocarbamide Trixton X-100, MES in Cleancell, increases it to condensing
The cleaning performance of the composition such as block and fibrin, and the preservatives of Proclin 300 are added, further safeguard electrode.
Develop and improve the Procell being formulated and Cleancell replacements original-pack Procell and Cleancell, to reduce poison
Property, cleaning performance is improved, further guard electrode reduces cost, has larger profit margin.
Specifically, the present invention relates to following four aspects, more specifically, one side, the present invention relates to one kind
Matching used electrochemical luminescence buffer solution and cleaning fluid, buffer composition include：Sodium dihydrogen phosphate, tripropyl amine (TPA), sodium chloride, grass
Acid, Tween 20, Proclin 300；Cleaning fluid component includes：Potassium hydroxide, Trixton X-100, MES, Proclin
300。
Preferably, its component of buffer solution therein and content are：250~350mmol/L of potassium dihydrogen phosphate, tripropyl amine (TPA) 60~
90mmol/L, 1~10mmol/L of sodium chloride, oxalic acid 3~10mmol/L, Tween 20 0.05~0.2% (w%), Proclin
300 0.05~0.2% (w%), pH value 6.6~7.0；Cleaning fluid component and content therein are：Potassium hydroxide 150-
200mmol/L, Trixton X-100 0.1~1% (w%), MES 0.1~1% (w%), Proclin 300 0.1~
0.5% (w%).
Second aspect, the present invention relates to a kind of matching used electrochemical luminescence buffer solution and cleaning fluid, buffer solution group
Dividing includes：Sodium dihydrogen phosphate, tripropyl amine (TPA), sodium chloride, PEG 300, Tween 20, Proclin 300, paracresol；Cleaning fluid group
Dividing includes：Potassium hydroxide, Trixton X-100, MES, urea, Proclin 300.
Preferably, its component of buffer solution therein and content are：250~350mmol/L of potassium dihydrogen phosphate, tripropyl amine (TPA) 20~
30mmol/L, sodium chloride 1~10mmol/L, PEG300 1~10mmol/L, Tween 20 0.05~0.2% (w%),
Proclin 3000.05~0.2% (w%), 0.1~1mmol/L of paracresol, pH value 6.6~7.0；Cleaning fluid component therein
It is with content：Potassium hydroxide 150-200mmol/L, Trixton X-100 0.1~1% (w%), MES/AES 0.1~1%
(w%), urea 0.1~1% (w%), Proclin300 0.1~0.5% (w%).
3rd aspect, the present invention relates to a kind of matching used electrochemical luminescence buffer solution and cleaning fluid, buffer solution group
Dividing includes：Sodium dihydrogen phosphate, tripropyl amine (TPA), N, N- bis- [just] butylamino ethanol, sodium chloride, PEG 300, malonyl urea, Tween
20, Proclin 300, paracresol, polyvinylpyrrolidone, methylcellulose；Cleaning fluid component includes：Potassium hydroxide,
Trixton X-100, MES, thiocarbamide, Proclin 300.
Preferably, its component of buffer solution therein and content are：250~350mmol/L of potassium dihydrogen phosphate, tripropyl amine (TPA) 1~
10mmol/L, N, N- bis- [just] butylamino 1~3mmol/L of ethanol, sodium chloride 1~10mmol/L, PEG 300 1~
10mmol/L, malonyl urea 1~5mmol/L, Tween 20 0.05~0.2% (w%), Proclin 300 0.05~0.2%
(w%), 0.1~1mmol/L of paracresol, 0.1~1mmol/L of polyvinylpyrrolidone, methylcellulose 0.1~1mmol/L, pH
Value 6.6~7.0；Cleaning fluid component and content therein are：Potassium hydroxide 150-200mmol/L, Trixton X-100 0.1~
1% (w%), MES0.1~1% (w%), thiocarbamide 0.1~1% (w%), Proclin 300 0.1~0.5% (w%).
4th aspect is as follows the present invention relates to the compound method of above-mentioned buffer solution and cleaning fluid：
First, the preparation of chemiluminescent buffer：Composition in addition to tripropyl amine (TPA) is weighed to add in a small amount of water respectively and stirred
Mixing makes dissolving, add tripropyl amine (TPA) stirring standing after it is complete it is molten after be transferred in volumetric flask, constant volume, shake up, adjust pH value, produce.
2nd, the preparation of cleaning fluid：Appropriate amount is weighed according to the composition in formula, be dissolved in water mixing, lets cool, is transferred to
Volumetric flask, constant volume, shake up, produce.
Embodiment
Below by specific embodiment, the present invention is described in detail, but the purposes of these exemplary embodiments and
Purpose is only used for enumerating the present invention, not forms any type of any restriction to the real protection scope of the present invention, more non-to incite somebody to action
Protection scope of the present invention is confined to this.
Embodiment 1
A, the component of chemiluminescent buffer (Procell) and preparation：
Potassium dihydrogen phosphate 270mmol/L
Tripropyl amine (TPA) 60mmol/L
Sodium chloride 2mmol/L
Oxalic acid 5mmol/L
Tween 20 0.05% (w%)
Proclin 300 0.05% (w%)
PH value is adjusted to 6.6 with NaOH
Other compositions in addition to tripropyl amine (TPA) are weighed to add in a small amount of water respectively and are stirred to dissolve, tripropyl amine (TPA) is added and stirs
Mix standing after it is complete it is molten after move in volumetric flask, constant volume, shake up and produce.
B, the component of cleaning fluid (Cleancell) and preparation：
Potassium hydroxide 150mmol/L
Trixton X-100 0.5%
MES 0.5%
Proclin 300 0.3%
Appropriate amount is weighed according to the composition in formula, be dissolved in water mixing, lets cool, and is transferred to volumetric flask, constant volume, shakes up
Produce.
Embodiment 2
A, the component of buffer solution (Procell) and preparation：
Potassium dihydrogen phosphate 350mmol/L
Tripropyl amine (TPA) 25mmol/L
Sodium chloride 5mmol/L
PEG 300 5mmol/L
Tween 20 0.1% (w%)
Proclin 300 0.1% (w%)
Paracresol 0.2mmol/L
PH value is adjusted to 7.0 with NaOH
Other compositions in addition to tripropyl amine (TPA) are weighed to add in a small amount of water respectively and are stirred to dissolve, tripropyl amine (TPA) is added and stirs
Mix standing after it is complete it is molten after move in volumetric flask, constant volume, shake up and produce.
B, the component of cleaning fluid (Cleancell) and preparation：
Potassium hydroxide 200mmol/L
Trixton X-100 0.2%
AES 0.8%
Urea 0.1%
Proclin 300 0.5%
Appropriate amount is weighed according to the composition in formula, be dissolved in water mixing, lets cool, and is transferred to volumetric flask, constant volume, shakes up
Produce.
Embodiment 3
A, the component of buffer solution (Procell) and preparation：
Potassium dihydrogen phosphate 300mmol/L
Tripropyl amine (TPA) 5mmol/L
N, N- bis- [just] butylamino ethanol 3mmol/L
Sodium chloride 5mmol/L
PEG 300 3mmol/L
Malonyl urea 5mmol/L
Tween 20 0.1% (w%)
Proclin 300 0.05% (w%)
Paracresol 0.1mmol/L
Polyvinylpyrrolidone 0.1mmol/L
Methylcellulose 0.1mmol/L
PH value is adjusted to 6.8 with NaOH
Other compositions in addition to tripropyl amine (TPA) are weighed to add in a small amount of water respectively and are stirred to dissolve, tripropyl amine (TPA) is added and stirs
Mix standing after it is complete it is molten after move in volumetric flask, constant volume, shake up and produce.
B, the component of cleaning fluid (Cleancell) and preparation：
Potassium hydroxide 180mmol/L
Trixton X-100 0.8%
MES 0.2%
Thiocarbamide 0.5%
Proclin 300 0.5%
Appropriate amount is weighed according to the composition in formula, be dissolved in water mixing, lets cool, and is transferred to volumetric flask, constant volume, shakes up
Produce.
Embodiment 4
First, Accuracy Verification experiment is determined
1st, the Procell and Cleancell configured embodiment 1 is placed in the Elecsys2010 electrochemical luminescences of Roche
Immunoassay system carries out checking test on corresponding position, takes 30 patients serums to be tested, contrast and original-pack supporting examination
The correlation of agent Procell, Cleancell testing result.
As a result show, Procell, Cleancell of autogamy embodiment 1 and original-pack Procell, Cleancell performance one
Cause property is good.
2nd, the Procell and Cleancell configured embodiment 2 is placed in the Elecsys2010 electrochemical luminescences of Roche
Immunoassay system carries out checking test on corresponding position, takes 30 patients serums to be tested, contrast and original-pack supporting examination
The correlation of agent Procell, Cleancell testing result.
As a result show, autogamy embodiment 2Procell, Cleancell is consistent with original-pack Procell, Cleancell performance
Property is good.
3rd, the Procell and Cleancell configured embodiment 3 is placed in the Elecsys2010 electrochemical luminescences of Roche
Immunoassay system carries out checking test on corresponding position, takes 30 patients serums to be tested, contrast and original-pack supporting examination
The correlation of agent Procell, Cleancell testing result.
As a result show, autogamy embodiment 3Procell, Cleancell is consistent with original-pack Procell, Cleancell performance
Property is good.
It in summary it can be seen, show Self-made reagent in the case where greatly reducing tripropyl amine (TPA) concentration, while remain to complete
Immunoassay on fully-automatic analyzer, meet the goldstandard with original-pack reagent uniformity, and result is accurately and reliably, it is seen that this
Procell, Cleancell of the improvement formula of invention have quite excellent safety and reliability.
Embodiment 5
2nd, Cleancell cleaning performances checking test
The cleaning of pipe-line system and measuring cell and electrodes maintenance after Cleancell effect mainly measures every time, this
Invention improves formula, to improve its cleaning action to pipeline and measuring cell, to therein on the basis of the formula of original-pack reagent
Reaction solution is mainly that the albumen remained is cleaned, and first reacts a large amount of blank serums and thyroid-stimulating hormone kit, then will
Above reaction solution is sub-packed in blank cuvette, is stood 30min, is outwelled, and is existed using autogamy Cleancell and imported reagents
Cuvette cleaning (respectively carrying out 20 groups of parallel tests) is carried out in the case of identical addition, the water of equivalent is added after cleaning, is shaken
It is even, under protein maximum absorption wavelength 280nm, absorbance is tested, observes result.
1. the Cleancell configured in embodiment 1 is tested according to above verification method, as a result see the table below：
2. the Cleancell configured in embodiment 2 is tested according to above verification method, as a result see the table below：
3. the Cleancell configured in embodiment 3 is tested according to above verification method, as a result see the table below：
It in summary it can be seen, autogamy embodiment 1-3, improve the Cleancell of formula compared with imported reagents
Cleancell albumen cleaning performance is more excellent, therefore the Cleancell of the improvement formula of the present invention, cleaning action enhancing.So as to
So that the matching used electrochemical luminescence buffer solution of the present invention and cleaning fluid will have more actual industrial application value.
It should be appreciated that the purposes of these embodiments is merely to illustrate the present invention and is not intended to the protection model of the limitation present invention
Enclose.In addition, it should also be understood that, after the technology contents of the present invention have been read, those skilled in the art can make each to the present invention
Kind change, modification and/or variation, all these equivalent form of values equally fall within the guarantor that the application appended claims are limited
Within the scope of shield.
Claims (2)
1. a kind of matching used electrochemical luminescence buffer solution and cleaning fluid, it is characterised in that chemiluminescent buffer therein
Its component and content are：250 ~ 350mmol/L of potassium dihydrogen phosphate, tripropyl amine (TPA) 1 ~ 10mmol/L, N, N- bis- [just] butyl ammonia
Base 1 ~ 3mmol/L of ethanol, sodium chloride 1 ~ 10mmol/L, PEG 300 1 ~ 10mmol/L, 1 ~ 5mmol/L of malonyl urea,
Tween 20 0.05~0.2% （w%）, Proclin 300 0.05 ~ 0.2%（w%）, the mmol/L of paracresol 0.1 ~ 1, polyethylene
The mmol/L of pyrrolidones 0.1 ~ 1, the mmol/L of methylcellulose 0.1 ~ 1, pH value 6.6 ~ 7.0；Cleaning fluid component therein and
Content is：Potassium hydroxide 150-200mmol/L, Trixton X-100 0.1 ~ 1%（w%）, MES 0.1 ~ 1%（w%）, thiocarbamide
0.1~1% （w%）, Proclin 300 0.1 ~ 0.5%（w%）.
2. buffer solution and cleaning fluid as described in claim 1, it is characterised in that its component of buffer solution therein and content
For：Potassium dihydrogen phosphate 300mmol/L, tripropyl amine (TPA) 5mmol/L, N, N- bis- [just] butylamino ethanol 3mmol/L, chlorination
Sodium 5mmol/L, PEG 300 3mmol/L, malonyl urea 5mmol/L, Tween 20 0.1%（w%）, Proclin 300
0.05%（w%）, paracresol 0.1mmol/L, the mmol/L of polyvinylpyrrolidone 0.1, the mmol/L of methylcellulose 0.1,
PH values 6.8；Cleaning fluid component and content therein are：Potassium hydroxide 180mmol/L, Trixton X-100 0.8%（w%）,
MES 0.2%（w%）, thiocarbamide 0.5%（w%）, Proclin 300 0.5%（w%）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610538476.XA CN106124753B (en) | 2016-07-08 | 2016-07-08 | Electrochemical luminescence buffer solution and cleaning fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610538476.XA CN106124753B (en) | 2016-07-08 | 2016-07-08 | Electrochemical luminescence buffer solution and cleaning fluid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106124753A CN106124753A (en) | 2016-11-16 |
| CN106124753B true CN106124753B (en) | 2018-03-06 |
Family
ID=57283630
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610538476.XA Active CN106124753B (en) | 2016-07-08 | 2016-07-08 | Electrochemical luminescence buffer solution and cleaning fluid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106124753B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109517672A (en) * | 2017-09-20 | 2019-03-26 | 刘宏飞 | A kind of Roche Electrogenerated chemiluminescent immunoassay instrument cleaning solution and preparation method thereof |
| CN108303528B (en) * | 2018-01-19 | 2021-03-02 | 青岛大学附属医院 | Substrate for electrochemical luminescence analyzer and preparation method and application thereof |
| KR20210146884A (en) | 2019-01-03 | 2021-12-06 | 메소 스케일 테크놀러지즈, 엘엘시 | Compositions and methods for performing assay measurements |
| CN111537706B (en) * | 2020-05-26 | 2021-05-28 | 长春晨裕生物医疗科技有限公司 | Immune lotion for electrochemical luminescence immunoassay instrument |
| CN111896731B (en) * | 2020-06-24 | 2023-06-09 | 宁波瑞源生物科技有限公司 | Cleaning solution for chemiluminescent immunoassay |
| CN112924438A (en) * | 2021-01-26 | 2021-06-08 | 金华市鑫科医疗器械有限公司 | Chemiluminescent cleaning buffer solution |
| CN116286205B (en) * | 2022-12-23 | 2024-04-19 | 浙江鑫科医疗科技有限公司 | Tripropylamine buffer solution matched with Luo Cobas e801 chemiluminescent immunoassay analyzer and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990005302A1 (en) * | 1988-11-03 | 1990-05-17 | Igen, Inc. | Enhanced electrochemiluminescence |
| JPH0534345A (en) * | 1991-02-19 | 1993-02-09 | Tdk Corp | Measuring method of antigen-antibody utilizing chemiluminescence |
| US5811236A (en) * | 1986-04-30 | 1998-09-22 | Igen International, Inc. | Electrochemiluminescent rhenium moieties and methods for their use |
| CN102755166A (en) * | 2012-06-20 | 2012-10-31 | 浙江大学 | Method for directly showing latent fingerprints based on multiple electrochemistry lighting systems |
| CN103443614A (en) * | 2010-10-25 | 2013-12-11 | 霍夫曼-拉罗奇有限公司 | Use of signal enhancing compounds in electrochemiluminescence detection |
| CN104360051A (en) * | 2014-11-28 | 2015-02-18 | 天津华信康达科技有限公司 | Substrate for electrochemiluminescence immunoassay and application thereof |
| CN104568927A (en) * | 2015-01-21 | 2015-04-29 | 山东师范大学 | Electrochemical luminescence device and method |
-
2016
- 2016-07-08 CN CN201610538476.XA patent/CN106124753B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5811236A (en) * | 1986-04-30 | 1998-09-22 | Igen International, Inc. | Electrochemiluminescent rhenium moieties and methods for their use |
| WO1990005302A1 (en) * | 1988-11-03 | 1990-05-17 | Igen, Inc. | Enhanced electrochemiluminescence |
| JPH0534345A (en) * | 1991-02-19 | 1993-02-09 | Tdk Corp | Measuring method of antigen-antibody utilizing chemiluminescence |
| CN103443614A (en) * | 2010-10-25 | 2013-12-11 | 霍夫曼-拉罗奇有限公司 | Use of signal enhancing compounds in electrochemiluminescence detection |
| CN102755166A (en) * | 2012-06-20 | 2012-10-31 | 浙江大学 | Method for directly showing latent fingerprints based on multiple electrochemistry lighting systems |
| CN104360051A (en) * | 2014-11-28 | 2015-02-18 | 天津华信康达科技有限公司 | Substrate for electrochemiluminescence immunoassay and application thereof |
| CN104568927A (en) * | 2015-01-21 | 2015-04-29 | 山东师范大学 | Electrochemical luminescence device and method |
Non-Patent Citations (2)
| Title |
|---|
| Roche 电化学发光免疫分析***发光缓冲液和清洗液配制及应用;肖静等;《检验医学与临床》;20060731;全文 * |
| Roche 电化学发光免疫分析***试剂Procell 及Cleancell 的配制及应用;周明欣;《临床医学工程》;20160531;全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106124753A (en) | 2016-11-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106124753B (en) | Electrochemical luminescence buffer solution and cleaning fluid | |
| Iwasaki et al. | New colorimetric determination of chloride using mercuric thiocyanate and ferric ion | |
| Bazelyansky et al. | Fractional diffusion-limited component of reactions catalyzed by acetylcholinesterase | |
| Pearson et al. | Mechanism of Substitution Reactions of Complex Ions. X. π-Bonding in Dissociation Reactions of Octahedral Complexes1, 2 | |
| Meot-Ner et al. | Internal and external solvation of polyfunctional ions | |
| CN109232589A (en) | A kind of fluorescence probe and its preparation and application | |
| JPS60501522A (en) | Reagent for whole blood dilution and hemolysis | |
| Salvato et al. | Acid-base titration of hemocyanin from Octopus vulgaris | |
| Creaser et al. | Outer-sphere electron-transfer reactions involving caged cobalt ions | |
| Richards et al. | Electrogenerated chemiluminescence. 57. Emission from sodium 9, 10-diphenylanthracene-2-sulfonate, thianthrenecarboxylic acids, and chlorpromazine in aqueous media | |
| McGarvey et al. | Detection of the EPR Spectra of NO• in Ruthenium (II) Complexes | |
| Hofstee | Nonlogarithmic linear titration curves | |
| Weaver et al. | Solvent isotope effects upon the thermodynamics of some transition-metal redox couples in aqueous media | |
| CN109517670A (en) | A kind of Abbott Laboratories' chemical illumination immunity analysis instrument cleaning solution and preparation method thereof | |
| CN112362432B (en) | Dissociation agent universally used in serum TT3 and TT4 and preparation method thereof | |
| Michalowski et al. | An unified quantitative approach to electrolytic systems | |
| CN105928912A (en) | Heparin detection method | |
| CN106226539B (en) | electrochemical luminescence cleaning buffer solution | |
| JPS6270205A (en) | Stabilized aqueous hydrogen peroxide solution | |
| CN103424395A (en) | Method for detecting medicine components in plasma | |
| Konteatis et al. | Stereoselectivity in lanthanide complexes of malic acid | |
| Ramesh et al. | Critical cation balance in B→ Z transition: role of Li+ | |
| Weit et al. | Contrasting reactivities of ligand-to-metal charge transfer excited states in ammine and methylamine complexes of cobalt (III) | |
| Kumooka et al. | Reversed micellar-mediated luminol chemiluminescence reaction with bis (acetylacetonato) oxovanadium (IV) | |
| Hung et al. | Oxidation of Thioglycolate by [Os (phen) 3] 3+: an unusual example of redox-mediated aromatic substitution |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |