CN106119325A - A kind of preparation method with the casein hydrolysate increasing Lean mass and strength effect - Google Patents
A kind of preparation method with the casein hydrolysate increasing Lean mass and strength effect Download PDFInfo
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- CN106119325A CN106119325A CN201610463117.2A CN201610463117A CN106119325A CN 106119325 A CN106119325 A CN 106119325A CN 201610463117 A CN201610463117 A CN 201610463117A CN 106119325 A CN106119325 A CN 106119325A
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
A kind of preparation method with the casein hydrolysate increasing Lean mass and strength effect, relates to the preparation method of a kind of casein hydrolysate.The present invention is to solve the edible peptide shortage in the market for " muscle decay disease ", the problem the most effectively increasing the albumen of Lean mass and strength.Method: one, be placed in distilled water by casein powder, stir, prepares casein suspension, regulates pH value, dissolves, add Alcalase alkaline protease, stir enzymolysis, hydrolyzed solution enzyme denaturing, be taken out to room temperature, add calcium chloride solution and ethanol, static, centrifugal, obtain supernatant；Two, supernatant is through using the mode of rotary evaporation to concentrate, and is dried, obtains casein hydrolysate powder in freezer dryer.Present invention casein hydrolysate increases Lean mass, and in the casein hydrolysate obtained, leucine content is more, and is prone to be digested and assimilated by organism.The present invention is used for preparing casein hydrolysate.
Description
Technical field
The present invention relates to the preparation method of a kind of casein hydrolysate.
Background technology
Showing according to correlational study, the whole nation tends to aging population day, especially Germany, such developed country of the U.S.,
China, such developing country of India, it is contemplated that the year two thousand fifty whole world population of more than 60 years old will more than 2,000,000,000 people, and wherein 500,000,000
Population is then in China.Show 50 years old according to domestic and international correlational study that again suffering from muscle decay disease crowd is 1%～2%；60～70 years old
Suffering from muscle decay disease crowd is 5%～13%；Within more than 80 years old, suffer from muscle decay disease crowd then more than 50%.They are tangible 20～30 years old years old
Time muscle quality relaxation phenomenon the most slowly start, and all one's life with people can be continued.Tool statistics is in U.S.'s muscle decay
The medical care expenses of the complication that disease causes is sufficiently expensive, and therefore regulation and improvement research to muscle decay are the most necessary.
The most single, both at home and abroad all based on lactalbumin to having the albumen research increasing Lean mass and strength at present
Object of study, the utilization of this high-quality protein is wanted to become a kind of trend.But abroad have and study individually display, lactalbumin not by
Proving the optimal albumen promoting muscle synthesis, old people takes in the lactalbumin effect with other albumen without significant difference,
Even below other albumen, lactalbumin is expensive relative to other albumen simultaneously.
Big for current muscle decay disease patient groups's ratio, cause not only to individual and family and have a strong impact on, more can give
Society, health delivery system bring heavy financial burden, but domestic correlational study is less.
Summary of the invention
The present invention is to solve the edible peptide shortage in the market for " muscle decay disease ", the most effectively increase flesh
The problem of the albumen of meat content and strength, it is provided that a kind of system with the casein hydrolysate increasing Lean mass and strength effect
Preparation Method.
The present invention has the preparation method of the casein hydrolysate increasing Lean mass and strength effect, enters according to the following steps
OK:
One, casein powder is placed in distilled water, stirs, prepare casein suspension, be 4mol/L by concentration
NaOH regulation solution ph to 7～8, dissolve in 50～60 DEG C of water-baths, when suspension without obvious granule and pH value without bright
During aobvious change, in casein suspension, add Alcalase alkaline protease, be 2mol/L by concentration in thermostat water bath
NaOH maintain casein hydrolyzate pH value to 7～8, and be slowly stirred enzymolysis 4h, the hydrolyzed solution obtained is in 100 DEG C of boiling water baths
Enzyme denaturing 10min, is taken out to room temperature, and (w/v) calcium chloride solution is 1.0% to final volume concentration to add 10%, (the v/ that adds 95% after dissolving
V) ethanol to final volume mark is 50%, and then static 1h is centrifuged 10min in 4000r/min, obtains supernatant；
Two, supernatant is through using the mode of rotary evaporation to concentrate, and is dried 24h, obtains casein in freezer dryer
Hydrolysate powder.
In step one, the concentration of casein suspension is 60-70mg/mL.
The volume of the Alcalase alkaline protease added in step one is the 0.5%～1% of casein suspension volume.
Alcalase alkaline protease in step one is bought from sigma company.
Beneficial effects of the present invention:
The present invention passes through Alcalase hydrolysis by novo casein, obtains producing phosphopeptide caseinate byproduct in process
Thing casein hydrolysate (casein non-phosphate peptides), and adequately and reasonably utilized, process skill not only for milk protein preparation
Art level and then widen its application category and there is important theory and more practical value, also to increasing Lean mass and the function of strength
Property a kind of new raw material of food development.
Increase Lean mass by the casein peptide that the inventive method is prepared and strength effect finds, raise after 50d
Feed the rat muscle group cross-sectional area of casein hydrolysate group more than non-fed casein hydrolysate group (blank group), trunk and lower limb
Root muscle group sectional area increment is maximum, more than blank group 1.71cm2, therefore casein hydrolysate group muscle cross sections amasss
Higher than blank group.
By feeding rat 10g/kg/d casein hydrolysate, after 50d, use CT scan technology, observe its muscle group horizontal
The muscle increment of sectional area and every rat 50d understands, and trunk and the lower limb root muscle group of fed casein hydrolysate group cut
Area, back muscle group sectional area, survey muscle group sectional area increment in omoplate and be all remarkably higher than blank group.
Present invention casein hydrolysate increases Lean mass, and in the casein hydrolysate obtained, leucine content is more,
And be prone to be digested and assimilated by organism.Digestion rate is fast compared with casein, and (leucine is to take in the raising of leucic usability
Albumen promotes the principal element of muscle synthesis).
Accompanying drawing explanation
Fig. 1 is the blank trunk organized with casein hydrolysate group and lower limb root muscle group cross-sectional area CT scan figure；
Fig. 2 is muscle group cross-sectional area CT scan figure around the lumbar vertebra of blank group and casein hydrolysate group；
Fig. 3 is muscle group cross-sectional area CT scan figure inside the omoplate of blank group and casein hydrolysate group.
Detailed description of the invention
Technical solution of the present invention is not limited to act detailed description of the invention set forth below, also includes between each detailed description of the invention
Combination in any.
Detailed description of the invention one: present embodiment has the system of the casein hydrolysate increasing Lean mass and strength effect
Preparation Method, sequentially includes the following steps:
One, casein powder is placed in distilled water, stirs, prepare casein suspension, be 4mol/L by concentration
NaOH regulation solution ph to 7～8, dissolve in 50～60 DEG C of water-baths, when suspension without obvious granule and pH value without bright
During aobvious change, in casein suspension, add Alcalase alkaline protease, be 2mol/L by concentration in thermostat water bath
NaOH maintain casein hydrolyzate pH value to 7～8, and be slowly stirred enzymolysis 4h, the hydrolyzed solution obtained is in 100 DEG C of boiling water baths
Enzyme denaturing 10min, is taken out to room temperature, and (w/v) calcium chloride solution is 1.0% to final volume concentration to add 10%, (the v/ that adds 95% after dissolving
V) ethanol to final volume mark is 50%, and then static 1h is centrifuged 10min in 4000r/min, obtains supernatant；
Two, supernatant is through using the mode of rotary evaporation to concentrate, and is dried 24h, obtains casein in freezer dryer
Hydrolysate powder.
Detailed description of the invention two: present embodiment is unlike detailed description of the invention one: in step one, casein is suspended
The concentration of liquid is 60-70mg/mL.Other is identical with detailed description of the invention one.
Detailed description of the invention three: present embodiment is unlike detailed description of the invention one: in step one, casein is suspended
The concentration of liquid is 65mg/mL.Other is identical with detailed description of the invention one.
Detailed description of the invention four: present embodiment is unlike one of detailed description of the invention one to three: use in step one
Concentration is that the NaOH regulation solution ph of 4mol/L is to 7.6.Other is identical with one of detailed description of the invention one to three.
Detailed description of the invention five: present embodiment is unlike one of detailed description of the invention one to four: in step one in
55 DEG C of water-baths dissolve.Other is identical with one of detailed description of the invention one to four.
Detailed description of the invention six: present embodiment is unlike one of detailed description of the invention one to five: add in step one
The volume of the Alcalase alkaline protease entered is the 0.5%～1% of casein suspension volume.Other and detailed description of the invention
One of one to five identical.
Detailed description of the invention seven: present embodiment is unlike one of detailed description of the invention one to five: add in step one
The volume of the Alcalase alkaline protease entered is the 0.7%～0.8% of casein suspension volume.Other and specific embodiment party
One of formula one to five is identical.
Detailed description of the invention eight: present embodiment is unlike one of detailed description of the invention one to seven: use in step one
Concentration is that the NaOH of 2mol/L maintains casein hydrolyzate pH value to 7.6.Other is identical with one of detailed description of the invention one to seven.
For checking beneficial effects of the present invention, carry out tests below:
Embodiment 1:
The present embodiment increases the preparation method of the casein hydrolysate of Lean mass and strength, sequentially includes the following steps:
One, 6.5g casein powder is placed in 100mL distilled water, stirs, prepare casein suspension, use concentration
Regulate solution ph to 7.6 for the NaOH of 4mol/L, dissolve in 55 DEG C of water-baths, when solution without obvious granule and pH value without bright
During aobvious change, in casein suspension, add Alcalase alkaline protease, be 2mol/L by concentration in thermostat water bath
NaOH maintain casein hydrolyzate pH value to 7.6 and be slowly stirred enzymolysis 4h, hydrolyzed solution is enzyme denaturing in 100 DEG C of boiling water baths
10min, is taken out to room temperature, (w/v) calcium chloride solution that adds 10%, is 1.0% to final volume concentration, adds 95% (v/v) after dissolving
Ethanol to final volume mark is 50%, static 1h.Hydrolyzed solution 4000r is centrifuged 10min, obtains supernatant.
Two, supernatant is through using the mode of rotary evaporation to concentrate, and is dried 24h, obtains casein in freezer dryer
Hydrolysate powder.
The volume of the Alcalase alkaline protease added in step one is the 0.5% of casein suspension volume.
Alcalase alkaline protease in step one is bought from sigma company.
The casein hydrolysate preparing present embodiment carries out following performance measurement:
1. the mensuration of casein hydrolysate yield:
Use dry weight algoscopy that the quality of casein hydrolysate is measured, calculate productivity as follows.
Result: casein hydrolysate yield is 83.63%.
2. increasing Lean mass and the mensuration of strength, concrete grammar is as follows:
CT Scanning Technology detection is used to feed 0,15,25,35,50 days muscle cross sections of rat casein hydrolysate
Long-pending.After detection method is rat anesthesia, the prostrate cross-section Spiral CT scan of row body, Scan slice thickness 0.8cm, rebuilds thickness 0.5cm.
According to the bony landmark of rat, every rat selects identical contrast aspect, every rat to select 6 aspects (to include: ramus of ischium water
Flat, crista iliaca upper limb level, psoas major upper limb level, clavicle total length level, upper limb root aspect (left and right)), then at selected body
In layer CT image, draw a circle to approve specific muscle group with closed curve, measure its sectional area.
Before 0d-blank group and 0d-casein hydrolysate group refer to not start to do experiment, two groups of random assortment, it is labeled as
Blank group and casein hydrolysate group.50d-blank group: every day only feeds national standard Rodent Diet；50d-casein hydrolysis
Thing group: also need to supplement the casein hydrolysis of 10g/kg/d to every rat while feeding national standard Rodent Diet every day
Thing, continues 50d.
Blank group and the trunk of casein hydrolysate group and lower limb root muscle group cross-sectional area CT scan figure as it is shown in figure 1,
In Fig. 1, A is 0d-blank group, and B is 0d-casein hydrolysate group, and C is 50d-blank group, and D is 50d-casein hydrolysate group.Empty
Muscle group cross-sectional area CT scan figure around the lumbar vertebra of white group and casein hydrolysate group as in figure 2 it is shown, in Fig. 2 A be 0d-blank
Group, B is 0d-casein hydrolysate group, and C is 50d-blank group, and D is 50d-casein hydrolysate group.Blank group and casein water
Solving muscle group cross-sectional area CT scan figure inside the omoplate of thing group as it is shown on figure 3, A is 0d-blank group in Fig. 3, B is 0d-casein
Hydrolysate group, C is 50d-blank group, and D is 50d-casein hydrolysate group.
It is evaluated when casein hydrolysate increases Lean mass and strength, fed casein hydrolysate rat
Trunk and lower limb root muscle group, around lumbar vertebra, muscle group and back muscle group sectional area increment are above blank group.Wherein trunk
And lower limb root muscle group sectional area increment is maximum, more than blank group 1.71cm2。
Table 1 rat muscle cross-sectional area increment
Claims (8)
1. a preparation method with the casein hydrolysate increasing Lean mass and strength effect, it is characterised in that the method
Sequentially include the following steps:
One, casein powder is placed in distilled water, stirs, prepare casein suspension, be 4mol/L's by concentration
NaOH regulation solution ph, to 7～8, is dissolved, when suspension without obvious granule and pH value without substantially in 50～60 DEG C of water-baths
During change, in casein suspension, add Alcalase alkaline protease, be 2mol/L's by concentration in thermostat water bath
NaOH maintenance casein hydrolyzate pH value is to 7～8, and stirs enzymolysis 4h, and the hydrolyzed solution obtained is enzyme denaturing in 100 DEG C of boiling water baths
10min, is taken out to room temperature, and (w/v) calcium chloride solution is 1.0% to final volume concentration to add 10%, (v/v) second that adds 95% after dissolving
Alcohol to final volume mark is 50%, and then static 1h is centrifuged 10min in 4000r/min, obtains supernatant；
Two, supernatant is through using the mode of rotary evaporation to concentrate, and is dried 24h, obtains casein hydrolysis in freezer dryer
Thing powder.
A kind of preparation side with the casein hydrolysate increasing Lean mass and strength effect the most according to claim 1
Method, it is characterised in that in step one, the concentration of casein suspension is 60-70mg/mL.
A kind of preparation side with the casein hydrolysate increasing Lean mass and strength effect the most according to claim 1
Method, it is characterised in that in step one, the concentration of casein suspension is 65mg/mL.
A kind of preparation side with the casein hydrolysate increasing Lean mass and strength effect the most according to claim 1
Method, it is characterised in that by the NaOH regulation solution ph that concentration is 4mol/L to 7.6 in step one.
A kind of preparation side with the casein hydrolysate increasing Lean mass and strength effect the most according to claim 1
Method, it is characterised in that dissolve in 55 DEG C of water-baths in step one.
A kind of preparation side with the casein hydrolysate increasing Lean mass and strength effect the most according to claim 1
Method, it is characterised in that the volume of the Alcalase alkaline protease added in step one is the 0.5% of casein suspension volume
～1%.
A kind of preparation side with the casein hydrolysate increasing Lean mass and strength effect the most according to claim 1
Method, it is characterised in that the volume of the Alcalase alkaline protease added in step one is the 0.7% of casein suspension volume
～0.8%.
A kind of preparation side with the casein hydrolysate increasing Lean mass and strength effect the most according to claim 1
Method, it is characterised in that maintain casein hydrolyzate pH value to 7.6 with the NaOH that concentration is 2mol/L in step one.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107397230A (en) * | 2017-08-21 | 2017-11-28 | 杭州特悘衡康生物科技有限公司 | Alimentation composition, the formula food for promoting Post operation muscle recovery and protein synthesis and its application |
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