CN106119147A - A kind of Methylotrophic bacillus cereus and biological control agent thereof and application - Google Patents
A kind of Methylotrophic bacillus cereus and biological control agent thereof and application Download PDFInfo
- Publication number
- CN106119147A CN106119147A CN201610395263.6A CN201610395263A CN106119147A CN 106119147 A CN106119147 A CN 106119147A CN 201610395263 A CN201610395263 A CN 201610395263A CN 106119147 A CN106119147 A CN 106119147A
- Authority
- CN
- China
- Prior art keywords
- biological control
- zdc
- control agent
- methylotrophic
- methylotrophic bacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Environmental Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention discloses a kind of Methylotrophic bacillus cereus and biological control agent thereof and application, belongs to Biological control plant disease technical field.Bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCC No.12218.Experiment shows, the Methylotrophic Bacillus strain ZDC 03 of the present invention has that fast growth, sporulation quantity be big, high-output stress-resistance and the feature such as can surely grow by rapid, high volume, therefore has a good application prospect.The biological control agent prepared by this Methylotrophic bacillus cereus, can not only prevent and treat efficiently by the microbial plant disease of Botrytis cinerea, moreover it is possible to be effectively improved crop yield, is the biological control agent of a kind of great application prospect.The biological control agent of this strain can prevent and treat the microbial plant disease of Botrytis cinerea.
Description
Technical field
The present invention relates to Biological control plant disease technical field, particularly to a kind of Methylotrophic bacillus cereus and
Biological control agent and application.
Background technology
Botrytis cinerea Pseudomonas weak parasitic plant pathogenic fungi, can infect 200 various plants, not only infect at trophophase,
Can also endanger in storage period.The characteristic that the pathogen of Botrytis cinerea has that breeding is fast, host range is wide, pathogenicity is strong etc., is increasingly becoming
If the disease that vegetable at protected field produces.The pathogen of Botrytis cinerea mainly causes rotten, droop, plant seedlings and the canker transplanted
And the necrosis etc. of cane.Before fruit maturation, plant infection the pathogen of Botrytis cinerea can cause fruit tissue softening to be rotted, and has a strong impact on
Fruit growth, makes agricultural production impaired.Infecting the pathogen of Botrytis cinerea in storage transport after fruit maturation can cause fruit the most rotten
Rotten, cause huge economic loss.Therefore, the microbial gray mold of Botrytis cinerea become and have a strong impact on plant growing and adopt
One of the most fresh-keeping Major Diseases.
Preventing and treating to gray mold for many years relies primarily on chemical pesticide, but the environmental pollution brought along with chemical agent and residual
Allowance problem, long-term prescription causes the appearance of drug-fast strain in addition, makes preventing and treating extremely difficult.And cultivate the disease-resistant variety cycle
Long, efficiency is low, and available germ plasm resource is limited.
Biological control is to person poultry safety, free from environmental pollution, is not likely to produce the research of Drug resistance, correlation theory and technology day by day
Concerned.The Methylotrophic bacillus cereus of the present invention has that fast growth, sporulation quantity be big, high-output stress-resistance and energy
Enough rapid, high volumes such as grow surely at the feature, have a good application prospect.
Summary of the invention
The invention provides a kind of Methylotrophic bacillus cereus and biological control agent thereof and application, solve due to chemistry
The use of medicament and the environmental pollution that brings and residual quantity problem.
For solving above-mentioned technical problem, the technical solution used in the present invention is:
The bacterial strain that the present invention provides is Methylotrophic bacillus cereus (Bacillus methylotrophicus) ZDC-
03, it is to separate to obtain from the soil of red island, Qingdao of Shandong province Coastal beach, is preserved in China on March 16th, 2016 micro-
(being called for short CGMCC, address is BeiChen West Road, Chaoyang District, BeiJing City 1 at biological inoculum preservation administration committee's common micro-organisms center
Institute 3, Institute of Microorganism, Academia Sinica), preserving number is CGMCC No.12218.It has a following biological characteristics: bacterium
Body elongated rod shape, size is (3.7～5.2) μ m (13～35) μm, can move, and produces spore, and Gram’s staining is positive.At LB
Cultivating 48h for 25 DEG C in culture medium, bacterium colony is canescence, and circular, edge protuberance, opaque, colony diameter size is 1～1.5mm.
A kind of Methylotrophic Bacillus strain ZDC-03, the 16S rRNA gene sequence of this Methylotrophic bacillus cereus
It is classified as shown in SEQ-1.
The present invention to provide Methylotrophic Bacillus strain ZDC-03 microbial Biological control Botrytis cinerea
Application in plant disease.
The present invention also provides a kind of biological control agent, including the Methylotrophic bacillus cereus described in claim 1
(Bacillus methylotrophicus) bacterial strain ZDC-03.
The present invention a kind of method providing biological control agent, comprise the following steps:
(1) the Methylotrophic Bacillus strain ZDC-03 NA being saved in test tube slant or LB culture medium are carried out
Activation, is placed in 28-30 DEG C of constant incubator and cultivates 2-3d；Described NA culture medium prescription is beef extract 3g, peptone 10g,
Sodium chloride 5g, agar 15-20g, add water to 1000mL, pH and be adjusted to 7.0-7.2；Described LB culture medium prescription is tryptone 10g,
Yeast extract 5g, sodium chloride 10g, agar 15-20g, distilled water 1000mL, pH are adjusted to 7.0-7.2；
(2) spore of the Methylotrophic Bacillus strain ZDC-03 of step (1) is transplanted to NA or LB liquid culture
In base, 28-30 DEG C of shaking table shaken cultivation 24h obtains seed liquor；Described NA liquid culture based formulas is beef extract 3g, albumen
Peptone 10g, sodium chloride 5g, add water to 1000mL, pH and be adjusted to 7.0-7.2；Described LB liquid culture based formulas is: tryptone 10g,
Yeast extract 5g, sodium chloride 10g, distilled water 1000mL, pH are adjusted to 7.0-7.2；
(3) by the volume ratio of 3.0%, seed liquor is inoculated in bulk fermentation culture medium, cultivation temperature 28-30 DEG C, cultivates
Time 36h, ventilating ratio 1:0.8；Fermentation liquid is obtained after having fermented；Described bulk fermentation culture medium prescription (weight ratio) is yellow
Semen Glycines powder 3%, Semen Maydis powder 3%, NaCl 0.1%, CaCO3 0.2%, remaining is water；
(4) the Methylotrophic fermentation of bacillus liquid of step (3) is mixed homogeneously with substrate, be dried；Described
Ferment liquid is 1:1 with the mass ratio of fermentation liquid substrate.
The present invention also provides biological control agent application in the preventing and treating microbial plant disease of Botrytis cinerea.The party
Method is to carry out spraying treatment in growing process；Described spraying is bacteria suspension or the fermentation of this Methylotrophic bacillus cereus
Liquid or metabolite or the biological control agent of preparation.
Beneficial effects of the present invention:
Experiment shows, the Methylotrophic Bacillus strain ZDC-03 of the present invention has fast growth, sporulation quantity
Greatly, high-output stress-resistance and the feature such as can surely grow by rapid, high volume, therefore have a good application prospect.By this Methylotrophic bud
Biological control agent prepared by spore bacillus, can not only prevent and treat efficiently by the microbial plant disease of Botrytis cinerea, moreover it is possible to have
Effect improves crop yield, is the biological control agent of a kind of great application prospect.
Detailed description of the invention
Below technical scheme is clearly and completely described, it is clear that described content is only this
Bright a part of embodiment rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art are not having
Have and make the every other embodiment obtained under creative work premise, broadly fall into the scope of protection of the invention.
Case study on implementation 1
1, the isolation and purification of Methylotrophic bacillus cereus (Bacillus methylotrophicus) ZDC-03
Methylotrophic bacillus cereus (Bacillus methylotrophicus) ZDC-03 of the present invention uses flat board to draw
Collimation method separates and obtains, and concrete separation method is: weigh 1g soil sample in 99mL sterilized water, be placed in shaking table vibration 5-10min, make
Soil sample is sufficiently mixed with water, i.e. obtains 10-2Soil dilution liquid.Take 1ml above-mentioned soil dilution liquid in 9ml sterilized water, shake up i.e.
Obtain 10-3Soil dilution liquid.Identical method configuration 10-4、10-5、10-6Soil dilution liquid.Variable concentrations is dipped with inoculating loop
Soil dilution liquid be repeated 3 times in flat lining out, each concentration.Culture dish is placed in the calorstat of 28-30 DEG C cultivation 24h,
The bacterium colony of the different shape in culture medium is rule in blank NA culture dish, purification Bacillus strain, number respectively
Preserve.
2, identification of strains
(1) microbiologic properties: thalline elongated rod shape, size is (3.7～5.2) μ m (13～35) μm, can move, and produces bud
Spore, Gram’s staining is positive.Cultivating 48h for 25 DEG C in LB culture medium, bacterium colony is canescence, circular, and edge protuberance is impermeable
Bright, colony diameter size is 1～1.5mm.(2) molecular biological characteristic
16S rRNA gene sequencing result (SEQ-1) of this bacterial strain is as follows:
ATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAAC
TCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAGACATAAAAGGTGGCTTCGGCTACCAC
TTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACG GCTCACCAAGGCGACGATGCGTAGCCGACCTG
AGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAA
TGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGA
ACAAGTGCCGTTCAAATAGGGCGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCG
CGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATG
TGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCA
CGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTG
AGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTA
GGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACT
CAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGG
TCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGT
CAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGG
GCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTG
GGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCT
CAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGA
ATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTT
TTAGGAGCCAGCC
Compare according to Gen-Bank sequence homology, bacterial strain ZDC-03 and Bacillus methylotrophicus sequence phase
It is 100% like property, therefore bacterial strain ZDC-03 is accredited as Methylotrophic bacillus cereus (Bacillus methylotrophicus).
Graw mold of tomato room effect is verified by embodiment 2 Methylotrophic Bacillus strain ZDC-03
1, experimental technique
Use flat board opposite culture method, the botrytis cinerea (Botrytis cinerea) bacterium cake (8mm) for examination is moved
To flat board central authorities, at surrounding 2cm, inoculate ZDC-03 bacterial strain, be placed on 28-30 DEG C of constant incubator and cultivate, not inoculate spore
The culture dish of bacillus compares, and each process is repeated 3 times.In time compareing mycelia and cover with flat board, decussation method is used to measure bacterium
Fall diameter, antibacterial band diameter, calculates meansigma methods and relative inhibition.
2, result
As can be seen from Table 1, botrytis cinerea mycelial growth is had relatively by Methylotrophic Bacillus strain ZDC-03
Strong inhibitory action, a diameter of 1.45cm of antibacterial band, mycelial growth inhibition rate is 87.38%.
Table 1 Methylotrophic Bacillus strain ZDC-03 is to botrytis cinerea Indoor antibacterial determination of activity
Graw mold of tomato field efficacy is verified by example 3 Methylotrophic bacillus cereus ZDC-03 fermentation liquid
1.1 reagent agent
Methylotrophic bacillus cereus ZDC-03 fermentation liquid；50% procymidone wettable powder (commercially available).
1.2 for studying thing and controlling object:
For studying thing: Fructus Lycopersici esculenti, kind is that powder despot No. 3 is won in agriculture
Controlling object: gray mold
1.3 experimental field situations
Experimental field it is located at Shandong Shouguang City Fructus Lycopersici esculenti protecting field, prevented in Shouguang City's Fructus Lycopersici esculenti protecting field booth in 2015
Control graw mold of tomato test.The cultivation condition of all experimental plots (soil types, fertilising quantity, water) uniformity, and
Meet local agricultural production actual.
1.4 EXPERIMENTAL DESIGN and arrangement
This EXPERIMENTAL DESIGN Methylotrophic bacillus cereus ZDC-03 fermentation liquid 400 times, 600 times, 800 times, 50% is rotten mould
Profit wettable powder 800 times and clear water comparison totally 5 process, each community 10m2, be repeated 3 times, Gong15Ge community, each community with
Machine arranges.
1.5 test surveys and computational methods
7 days investigation investigation state of an illness radixes, whole test survey 2 times before laxative, after dispenser.Investigation method uses and takes out at random
Sample method is investigated.Every random 5 strains in community, 2 branch numberings of often selecting good strains in the field for seed are listed, all from fully expanded leaves several 5 after medicine prodrug
Sheet leaf is investigated, and every community investigates 50 altogether.With Duncan duncan's new multiple range method, test data is carried out statistical analysis.In dispenser
Period, observe each process and Fructus Lycopersici esculenti is produced with or without poisoning.
Stage division:
0 grade: without scab；
1 grade: lesion area accounts for whole leaf area less than 5%；
3 grades: lesion area accounts for whole leaf area 6%～10%；
5 grades: lesion area accounts for whole leaf area 11%～20%；
7 grades: lesion area accounts for whole leaf area 21%～50%
9 grades: lesion area accounts for more than the 51% of whole leaf area.
By following equation calculating disease index and prevention effect:
2. result
2.1 reagent agents prevention effect to graw mold of tomato
The table 2 Methylotrophic bacillus cereus ZDC-03 fermentation liquid field control effectiveness test to graw mold of tomato
Result of the test shows, after dispenser 7 days, anti-when Methylotrophic bacillus cereus ZDC-03 fermentation liquid dilutes 400 times
Control effect best, be 76.46%；When ZDC-03 fermentation liquid dilutes 600 times and 800 times there is not significant difference in preventive effect, respectively
It is 73.62%, 72.85%；During 50% procymidone wettable powder dilution agent 800 times, preventive effect is worst, and only 67.08%, the lowest
Process in Methylotrophic bacillus cereus ZDC-03 fermentation liquid.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Within god and principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (6)
1. Methylotrophic bacillus cereus (Bacillus methylotrophicus) bacterial strain ZDC-03, this bacterial strain preservation
In China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.12218.
A kind of Methylotrophic Bacillus strain ZDC-03 the most according to claim 1, this Methylotrophic spore bar
The 16S rRNA gene order of bacterium is shown in SEQ-1.
Methylotrophic Bacillus strain ZDC-03 the most according to claim 1 causes at Biological control the pathogen of Botrytis cinerea
Plant disease in application.
4. a biological control agent, including the Methylotrophic bacillus cereus described in claim 1
(Bacillusmethylotrophicus) bacterial strain ZDC-03.
5. the method preparing biological control agent described in claim 4, is characterized in that, comprise the following steps:
(1) the Methylotrophic Bacillus strain ZDC-03 NA being saved in test tube slant or LB culture medium are lived
Change, be placed in 28-30 DEG C of constant incubator and cultivate 2-3d；Described NA culture medium prescription is beef extract 3g, peptone 10g, chlorine
Change sodium 5g, agar 15-20g, add water to 1000mL, pH and be adjusted to 7.0-7.2；Described LB culture medium prescription is tryptone 10g, ferment
Female extract 5g, sodium chloride 10g, agar 15-20g, distilled water 1000mL, pH are adjusted to 7.0-7.2；
(2) spore of the Methylotrophic Bacillus strain ZDC-03 of step (1) is transplanted in NA or LB fluid medium,
28-30 DEG C of shaking table shaken cultivation 24h obtains seed liquor；Described NA liquid culture based formulas is beef extract 3g, peptone
10g, sodium chloride 5g, add water to 1000mL, pH and be adjusted to 7.0-7.2；Described LB liquid culture based formulas is: tryptone 10g, ferment
Female extract 5g, sodium chloride 10g, distilled water 1000mL, pH are adjusted to 7.0-7.2；
(3) by the volume ratio of 3.0%, seed liquor is inoculated in bulk fermentation culture medium, cultivation temperature 28-30 DEG C, incubation time
36h, ventilating ratio 1:0.8；Fermentation liquid is obtained after having fermented；Described bulk fermentation culture medium prescription (weight ratio) is analysis for soybean powder
3%, Semen Maydis powder 3%, NaCl 0.1%, CaCO3 0.2%, remaining is water；
(4) the Methylotrophic fermentation of bacillus liquid of step (3) is mixed homogeneously with substrate, be dried；Described fermentation liquid
It is 1:1 with the mass ratio of fermentation liquid substrate.
6. the application in the preventing and treating microbial plant disease of Botrytis cinerea of the biological control agent described in claim 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610395263.6A CN106119147A (en) | 2016-06-03 | 2016-06-03 | A kind of Methylotrophic bacillus cereus and biological control agent thereof and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610395263.6A CN106119147A (en) | 2016-06-03 | 2016-06-03 | A kind of Methylotrophic bacillus cereus and biological control agent thereof and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106119147A true CN106119147A (en) | 2016-11-16 |
Family
ID=57270057
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610395263.6A Pending CN106119147A (en) | 2016-06-03 | 2016-06-03 | A kind of Methylotrophic bacillus cereus and biological control agent thereof and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106119147A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929445A (en) * | 2017-03-15 | 2017-07-07 | 青岛秾贝尔生物工程有限公司 | One plant of methylotrophy bacillus KACC13105 and its application |
| CN107058183A (en) * | 2017-05-17 | 2017-08-18 | 山东省葡萄研究院 | A kind of Methylotrophic bacillus and its biocontrol agent and application |
| CN108315282A (en) * | 2018-04-17 | 2018-07-24 | 山东省林业科学研究院 | One plant of Methylotrophic bacillus and its application in preventing Chinese wax brown spot |
| CN109055257A (en) * | 2018-07-27 | 2018-12-21 | 山西省农业科学院农产品贮藏保鲜研究所 | It is a kind of produce volatility antibacterial material antagonistic bacterium and its application |
| CN111057659A (en) * | 2019-10-14 | 2020-04-24 | 陕西恒田生物农业有限公司 | Preparation and application of bacillus methylotrophicus LW-6 microbial inoculum for biologically preventing and treating shrimp diseases |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194415A (en) * | 2013-04-24 | 2013-07-10 | 牛赡光 | Bacillus subtilis and application thereof |
| CN105557756A (en) * | 2016-02-05 | 2016-05-11 | 中国农业科学院植物保护研究所 | Plant bacteriostasis method adopting bacillus methylotrophicus strain NKG-1 |
-
2016
- 2016-06-03 CN CN201610395263.6A patent/CN106119147A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194415A (en) * | 2013-04-24 | 2013-07-10 | 牛赡光 | Bacillus subtilis and application thereof |
| CN105557756A (en) * | 2016-02-05 | 2016-05-11 | 中国农业科学院植物保护研究所 | Plant bacteriostasis method adopting bacillus methylotrophicus strain NKG-1 |
Non-Patent Citations (2)
| Title |
|---|
| HONGYING SHAN等: "biocontrol of rice blast by the phenaminomethylacetic acid producer of bacillus methylotrophicus strain BC79", 《CROP PROTECTION》 * |
| 刘伟等: "番茄灰霉病拮抗芽孢杆菌LW-6-1的筛选、鉴定及抑菌活性研究", 《西北农林科技大学学报（自然科学版）》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929445A (en) * | 2017-03-15 | 2017-07-07 | 青岛秾贝尔生物工程有限公司 | One plant of methylotrophy bacillus KACC13105 and its application |
| CN106929445B (en) * | 2017-03-15 | 2020-06-05 | 青岛秾贝尔生物工程有限公司 | Bacillus methylotrophicus KACC13105 and application thereof |
| CN107058183A (en) * | 2017-05-17 | 2017-08-18 | 山东省葡萄研究院 | A kind of Methylotrophic bacillus and its biocontrol agent and application |
| CN107058183B (en) * | 2017-05-17 | 2021-03-12 | 山东省葡萄研究院 | Bacillus methylotrophicus, and biocontrol microbial inoculum and application thereof |
| CN108315282A (en) * | 2018-04-17 | 2018-07-24 | 山东省林业科学研究院 | One plant of Methylotrophic bacillus and its application in preventing Chinese wax brown spot |
| CN109055257A (en) * | 2018-07-27 | 2018-12-21 | 山西省农业科学院农产品贮藏保鲜研究所 | It is a kind of produce volatility antibacterial material antagonistic bacterium and its application |
| CN111057659A (en) * | 2019-10-14 | 2020-04-24 | 陕西恒田生物农业有限公司 | Preparation and application of bacillus methylotrophicus LW-6 microbial inoculum for biologically preventing and treating shrimp diseases |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102433282B (en) | Bacillus subtilis NB12, as well as culture method and application thereof | |
| CN103509740B (en) | Pseudomonas chlororaphis for preventing and treating crop fusarium disease and applications thereof | |
| CN103215209B (en) | Bacillus amyloliquefaciens and application thereof | |
| CN102851243A (en) | Paenibacillus polymyxa and applications thereof | |
| CN102876615B (en) | Rhodopseudomonas palustris strains, application of rhodopseudomonas palustris strains, fungicide pesticide as well as preparation method and application of fungicide pesticide | |
| CN106119147A (en) | A kind of Methylotrophic bacillus cereus and biological control agent thereof and application | |
| CN106754489A (en) | Methylotrophic bacillus F7 and its application | |
| CN109266589A (en) | Jie meter La series bacillus and its application of one plant of inhibition anthrax-bacilus and sickle-like bacteria | |
| CN107090419B (en) | Bacillus amyloliquefaciens and application thereof | |
| CN104946567A (en) | Bacillus atrophaeus and application thereof | |
| CN109207412A (en) | A kind of resistance to bacterial wilt biocontrol bacterial strain and its application | |
| CN103074272A (en) | Bacillus methylotrophicus Sanju-04 and its application | |
| CN104651260A (en) | Brevibacillus brevis BBC-3 and application thereof as well as preparation method of microbial inoculum of brevibacillus brevis | |
| CN106119146A (en) | A kind of husky good fortune bacillus cereus and biological control agent thereof and application | |
| CN107267412A (en) | Methylotrophic bacillus and its application | |
| CN105647829A (en) | Bacillus amyloliquefaciens and application thereof to prevention and treatment of sugarcane red rot | |
| CN105746503A (en) | Wettable Bacillus methylotrophicus powder and preparation method and application thereof | |
| CN105199996A (en) | Bacillus amyloliquefaciens for controlling tomato gray mould and application of bacillus amyloliquefaciens | |
| CN106942276A (en) | Bacillus subtilis BAB 1 prevents and treats the application of cucurbits powdery mildew | |
| CN103667099B (en) | A kind of subtilis for preventing and treating crop fusarium disease and application thereof | |
| CN104988097B (en) | A kind of streptomycete ER1396 bacterial strains and purposes | |
| CN104673720B (en) | A kind of bacillus megaterium and its suspending agent for being used to prevent and treat downy mildew of garpe | |
| CN103555615B (en) | A kind of bacillus amyloliquefaciens and uses thereof | |
| CN105018393A (en) | Bacillus megaterium strain and application thereof | |
| CN102443559B (en) | Baclillus subtilis used for controlling cotton verticillium wilt and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161116 |