CN106119079A - Clearance flow cyto-mechanics biology experimental installation between one - Google Patents
Clearance flow cyto-mechanics biology experimental installation between one Download PDFInfo
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- CN106119079A CN106119079A CN201610524616.8A CN201610524616A CN106119079A CN 106119079 A CN106119079 A CN 106119079A CN 201610524616 A CN201610524616 A CN 201610524616A CN 106119079 A CN106119079 A CN 106119079A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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Abstract
The present invention relates to cyto-mechanics biology techniques field, it is specifically related to clearance flow cyto-mechanics biology experimental installation between one, it includes experimental provision body, described experimental provision body includes platen, the described platen surface left and right sides is correspondingly arranged on left through hole and right through hole, platen surface corner is provided with four table top screws, twists and be rotary with four table top bolts in four table top screws, and the bottom of table top bolt is provided with a spike cap；Experiment box A and experiment box B it is respectively arranged with on the left through hole of described platen and right through hole；It has simple in construction, low cost, workable, clearance flow shearing force between varying strength is loaded by syringe pump, research transparent cup inner cell is to the response of clearance flow shearing force between simulation, thus simulates people's in-vivo tissue gap stream, identical or different cell type applies clearance flow shearing force between controlled size and loads, between research cell pair, response and the correlation molecule of clearance flow shearing force are machine-processed, represent the Developing trend of new technology of cyto-mechanics field of biology.
Description
[technical field]
The present invention relates to cyto-mechanics biology techniques field, be specifically related to clearance flow cyto-mechanics biological experiment dress between one
Put.
[background technology]
Cytobiology is with cell as object of study, from the integral level of cell, sub-microscopic level, three layers of molecular water equality
Secondary, with dynamic viewpoint, the research 26S Proteasome Structure and Function of cell and crganelle, the life cycle of cell and various vital movement rule
Subject.Cyto-mechanics inquires into mechanical environment mainly by the combination of cytobiology with mechanics principle method biology
(stimulation) impact on cell, the mechanical signal impression of research cell and response mechanism and the mechanical process of participation body thereof and life
The molecular mechanism of thing process such as growth, reconstruction, adaptations and reparation etc., thus develop medicable or have diagnostic significance
New technique, promote the development of biomedical basic and clinic studies, promote human health.
Intracellular fluid is intracellular liquid.Space between tissue, cell is referred to as interstice, wherein for tissue fluid institute
It is full of.Tissue fluid is the environment that tissue, cell are directly residing.Tissue, cell are by cell membrane and tissue fluid generation mass exchange.
Then mass exchange is carried out by capillary wall between tissue fluid and blood.
The circulation of people's in-vivo tissue gap stream is the mechanical environment (stimulation) that body be can not ignore.Between simulation people's in-vivo tissue
Clearance flow environment (stimulates), identical or different cell type applies clearance flow shearing force between controlled size and loads, study cell pair
Between the response of clearance flow shearing force and correlation molecule mechanism be the Developing trend of new technology in cell biomechanics field.But, currently
People for applying clearance flow shearing force between controlled size how to identical or different cell type load, and the most preferably study
Cell, for a response of clearance flow shearing force and relevant molecular mechanism, lacks applicable gap flow field simulation device.
[summary of the invention]
Present invention aims to defect and the deficiency of prior art, it is provided that a kind of simple in construction, reasonable in design, user
Clearance flow cyto-mechanics biology experimental installation between one just, it uses the platen of a band through hole, arranges arc on platen
Box, is provided with transparent cup, arc box is provided with the inflow tube of band syringe pump, platen is provided with outflow tube in arc box
Road；It has a simple in construction, and low cost is workable, loads clearance flow shearing force between varying strength, research by syringe pump
Transparent cup inner cell is to the response of clearance flow shearing force between simulation, thus simulates people's in-vivo tissue gap stream, to identical or different
Cell type applies clearance flow shearing force between controlled size and loads, and studies response and the correlation molecule of clearance flow shearing force between cell pair
Mechanism, represents the Developing trend of new technology of cyto-mechanics field of biology.
Clearance flow cyto-mechanics biology experimental installation between one of the present invention, it includes experimental provision body, described
Experimental provision body includes that platen, the described platen surface left and right sides are correspondingly arranged on left through hole and right through hole, platen surface four
Angle is provided with four table top screws, twists and be rotary with four table top bolts in four table top screws, and the bottom of table top bolt is provided with props up
Spike cap；
Experiment box A and experiment box B it is respectively arranged with on the left through hole of described platen and right through hole；
Described experiment box A includes arc back box A, arc back box A inner bottom plating A is provided with through hole A, through hole A logical with a left side for platen
Hole is corresponding, is provided with clear cell cup A in arc back box A, and arc back box A is provided with the arc lid A matched, arc
Lid A is fixed in arc back box A by bolt A；Described arc lid A surface middle part is provided with intermediate throughholes A, arc lid
Convex dish A it is provided with in the middle part of A bottom surface；It is provided with inflow tube A, this inflow tube A in intermediate throughholes A of described arc lid A surface
Inlet tube end be provided with syringe pump A；
Described experiment box B includes arc back box B, arc back box B inner bottom plating B is provided with through hole B, through hole B logical with the right side of platen
Hole is corresponding, is provided with clear cell cup B in arc back box B, and arc back box B is provided with the arc lid B matched, arc
Lid B is fixed in arc back box B by bolt B；It is provided with intermediate throughholes B, arc lid in the middle part of described arc lid B surface
Convex dish B it is provided with in the middle part of B bottom surface；It is provided with inflow tube B, this inflow tube B in intermediate throughholes B of described arc lid B7 surface
Inlet tube end be provided with syringe pump B；
Effluent conduit A and effluent conduit B it is respectively arranged with on the left through hole of described platen and right through hole；Described clear cell cup A
Be respectively arranged with cell A and cell B in clear cell cup B.
Further, described clear cell cup A includes that the rim of a cup surrounding of cup A, cup A is evenly arranged with three lug A.
Further, described clear cell cup B includes that the rim of a cup surrounding of cup B, cup B is evenly arranged with three lug B.
Further, the through hole A surrounding of the bottom surface of described arc back box A is provided with sealing annular groove, and this sealing annular groove sets
It is equipped with waterstop sealing circle.
After using said structure, present invention have the beneficial effect that clearance flow cyto-mechanics biology between one of the present invention
Experimental provision, it has a simple in construction, low cost, workable, loads clearance flow between varying strength by syringe pump and shears
Power, research transparent cup inner cell is to the response of clearance flow shearing force between simulation, thus simulates people's in-vivo tissue gap stream, to identical
Or dissimilar cell applies clearance flow shearing force between controlled size and loads, the response of clearance flow shearing force and phase between research cell pair
Close molecular mechanism, represent the Developing trend of new technology of cyto-mechanics field of biology.
[accompanying drawing explanation]
Accompanying drawing described herein is used to provide a further understanding of the present invention, does not constitutes the part of the application, but not
Constitute inappropriate limitation of the present invention, in the accompanying drawings:
Fig. 1 is present configuration schematic diagram；
Fig. 2 is the structural representation of the removing arc lid A of the present invention；
Fig. 3 is the structural representation of the platen of the present invention；
Fig. 4 is the structural representation of the arc lid A of the present invention；
Fig. 5 is the structural representation of arc back box A of the present invention.
Description of reference numerals:
1, experimental provision body；2, platen；21, left through hole；22, right through hole；3, arc back box A；31, base plate A；32, through hole A；
33, sealing annular groove；4, arc back box B；5, table top bolt；6, inflow tube B；7, arc lid B；8, inflow tube A；9, bolt
A；10, arc lid A；101, intermediate throughholes A；102, convex dish A；11, clear cell cup A.
[detailed description of the invention]
Only the present invention, illustrative examples therein and explanation is described in detail below in conjunction with accompanying drawing and specific embodiment
It is used for explaining the present invention, but not as a limitation of the invention.
As Figure 1-Figure 5, clearance flow cyto-mechanics biology experimental installation between the one described in this detailed description of the invention, it
Including experimental provision body 1, described experimental provision body 1 includes that platen 2, the left and right sides, described platen 2 surface are correspondingly arranged on
Left through hole 21 and right through hole 22, corner, platen 2 surface is provided with four table top screws, twists and be rotary with four platforms in four table top screws
Face bolt 5, the bottom of table top bolt 5 is provided with a spike cap；
Experiment box A and experiment box B it is respectively arranged with on the left through hole 21 of described platen 2 and right through hole 22；
Described experiment box A includes arc back box A3, and arc back box A3 inner bottom plating A31 is provided with through hole A32, through hole A32 and platform
The left through hole 21 of plate 2 is corresponding, is provided with clear cell cup A11 in arc back box A3, arc back box A3 is provided with and matches
Arc lid A10, arc lid A10 be fixed in arc back box A3 by bolt A9；Described arc lid A10 surface middle part
Being provided with intermediate throughholes A101, arc lid A10 is provided with convex dish A102 in the middle part of bottom surface；In the middle of described arc lid A10 surface
Being provided with inflow tube A8 on through hole A101, the inlet tube end of this inflow tube A8 is provided with syringe pump A；
Described experiment box B includes arc back box B4, and arc back box B4 inner bottom plating B is provided with the right side of through hole B, through hole B and platen 2
Through hole 22 is corresponding, is provided with clear cell cup B in arc back box B4, and arc back box B4 is provided with the arc lid matched
B7, arc lid B7 are fixed in arc back box B4 by bolt B；Described arc lid B7 surface middle part is provided with intermediate throughholes
B, arc lid B7 are provided with convex dish B in the middle part of bottom surface；It is provided with inflow tube in intermediate throughholes B of described arc lid B7 surface
B6, the inlet tube end of this inflow tube B6 is provided with syringe pump B；
Effluent conduit A and effluent conduit B it is respectively arranged with on the left through hole 21 of described platen 2 and right through hole 22；Described transparent carefully
It is respectively arranged with cell A and cell B in born of the same parents cup A11 and clear cell cup B.
One as the present invention is preferred, and described clear cell cup A11 includes that the rim of a cup surrounding of cup A, cup A uniformly sets
It is equipped with three lug A.
One as the present invention is preferred, and described clear cell cup B includes that the rim of a cup surrounding of cup B, cup B is uniformly arranged
There are three lug B.
One as the present invention is preferred, and the through hole A32 surrounding of the bottom surface of described arc back box A3 is provided with sealing annular groove
33, this sealing annular groove 33 is provided with waterstop sealing circle.
In the present invention, the left through hole 21 of platen 2 and right through hole 22 are laid experiment box A and experiment box B respectively, tests box A
It is the experiment box body identical with dimensional structure with experiment box B.Clear cell cup A11 and transparent in experiment box A and experiment box B
It is separately added into cell A and cell B in cell cup B, is then come by the inflow tube A8 on experiment box A by control syringe pump A
Load clearance flow shearing force between different sizes；The same syringe pump B that controls is added by the inflow tube B6 on experiment box B
Carry clearance flow shearing force between different sizes；Then recycling effluent conduit A and effluent conduit B flows out, and forms circulation；To identical
Cell or different cell apply clearance flow shearing force between different size and load, and follow-up cell after having stimulated is carried out cell divide
Sub-Mechanism Study, thus realize simulating people's in-vivo tissue gap stream, the response of clearance flow shearing force and relevant point between research cell pair
The purpose of handset.
In the present invention, the rim of a cup surrounding of clear cell cup A11 is evenly arranged with three lug A, the rim of a cup of clear cell cup B
Surrounding is evenly arranged with three lug B, and the function of lug A and lug B is used to increase cup stability.
In the present invention, the through hole A32 surrounding of the bottom surface of arc back box A3 is provided with sealing annular groove 33, and sealing annular groove 33 sets
It is equipped with waterstop sealing circle so that the design has leak-stopping drip-preventing function.
In the present invention, clear cell cup A11 uses biological semi-permeable film preparation to form.
Clearance flow cyto-mechanics biology experimental installation between one of the present invention, it has a simple in construction, low cost, can
Strong operability, loads clearance flow shearing force between varying strength by syringe pump, and clearance flow between simulation is cut by research transparent cup inner cell
The response of shear force, thus simulate people's in-vivo tissue gap stream, identical or different cell type is applied the gap of controlled size
Stream shearing force loads, the response of clearance flow shearing force and correlation molecule mechanism between research cell pair, represents cyto-mechanics biology
The Developing trend of new technology in field.
The above is only the better embodiment of the present invention, thus all according to the structure described in present patent application scope,
The equivalence that feature and principle are done changes or modifies, in the range of being all included in present patent application.
Claims (4)
1. clearance flow cyto-mechanics biology experimental installation between a kind, it is characterised in that: it includes experimental provision body, described experiment
Device body includes that platen, the described platen surface left and right sides are correspondingly arranged on left through hole and right through hole, and platen surface corner sets
Being equipped with four table top screws, twist and be rotary with four table top bolts in four table top screws, the bottom of table top bolt is provided with a spike
Cap；
Experiment box A and experiment box B it is respectively arranged with on the left through hole of described platen and right through hole；
Described experiment box A includes arc back box A, arc back box A inner bottom plating A is provided with through hole A, through hole A logical with a left side for platen
Hole is corresponding, is provided with clear cell cup A in arc back box A, and arc back box A is provided with the arc lid A matched, arc
Lid A is fixed in arc back box A by bolt A；Described arc lid A surface middle part is provided with intermediate throughholes A, arc lid
Convex dish A it is provided with in the middle part of A bottom surface；It is provided with inflow tube A, this inflow tube A in intermediate throughholes A of described arc lid A surface
Inlet tube end be provided with syringe pump A；
Described experiment box B includes arc back box B, arc back box B inner bottom plating B is provided with through hole B, through hole B logical with the right side of platen
Hole is corresponding, is provided with clear cell cup B in arc back box B, and arc back box B is provided with the arc lid B matched, arc
Lid B is fixed in arc back box B by bolt B；It is provided with intermediate throughholes B, arc lid in the middle part of described arc lid B surface
Convex dish B it is provided with in the middle part of B bottom surface；It is provided with inflow tube B, this inflow tube B in intermediate throughholes B of described arc lid B7 surface
Inlet tube end be provided with syringe pump B；
Effluent conduit A and effluent conduit B it is respectively arranged with on the left through hole of described platen and right through hole；Described clear cell cup A
Be respectively arranged with cell A and cell B in clear cell cup B.
Clearance flow cyto-mechanics biology experimental installation between one the most according to claim 1, it is characterised in that: described transparent
Cell cup A includes that the rim of a cup surrounding of cup A, cup A is evenly arranged with three lug A.
Clearance flow cyto-mechanics biology experimental installation between one the most according to claim 1, it is characterised in that: described transparent
Cell cup B includes that the rim of a cup surrounding of cup B, cup B is evenly arranged with three lug B.
Clearance flow cyto-mechanics biology experimental installation between one the most according to claim 1, it is characterised in that: described arc
The through hole A surrounding of the bottom surface of back box A is provided with sealing annular groove, and this sealing annular groove is provided with waterstop sealing circle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610524616.8A CN106119079B (en) | 2016-07-06 | 2016-07-06 | Clearance flow cyto-mechanics biology experimental installation between one kind |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610524616.8A CN106119079B (en) | 2016-07-06 | 2016-07-06 | Clearance flow cyto-mechanics biology experimental installation between one kind |
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| Publication Number | Publication Date |
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| CN106119079A true CN106119079A (en) | 2016-11-16 |
| CN106119079B CN106119079B (en) | 2018-04-17 |
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| CN201610524616.8A Active CN106119079B (en) | 2016-07-06 | 2016-07-06 | Clearance flow cyto-mechanics biology experimental installation between one kind |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106554902A (en) * | 2016-09-02 | 2017-04-05 | 四川大学 | A kind of power chemical Coupling biology experimental installation |
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| CN201834910U (en) * | 2010-09-21 | 2011-05-18 | 重庆文理学院 | Two-way perfusion mechanical experiment device |
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| CN102559492A (en) * | 2011-12-15 | 2012-07-11 | 北京航空航天大学 | Cell culture device capable of loading shear force stimulation and electrical stimulation jointly in parallel direction |
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| CN205133611U (en) * | 2015-10-30 | 2016-04-06 | 同济大学 | Continuous shearing stress generating device of cell equipartition |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106554902A (en) * | 2016-09-02 | 2017-04-05 | 四川大学 | A kind of power chemical Coupling biology experimental installation |
| CN106554902B (en) * | 2016-09-02 | 2019-03-05 | 四川大学 | A kind of power-chemical Coupling biology experimental installation |
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| Publication number | Publication date |
|---|---|
| CN106119079B (en) | 2018-04-17 |
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