CN106118877B - Antioxidant for cottonseed oil - Google Patents
Antioxidant for cottonseed oil Download PDFInfo
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- CN106118877B CN106118877B CN201610555407.XA CN201610555407A CN106118877B CN 106118877 B CN106118877 B CN 106118877B CN 201610555407 A CN201610555407 A CN 201610555407A CN 106118877 B CN106118877 B CN 106118877B
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
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Abstract
The invention discloses an antioxidant for cottonseed oil, which is prepared from the following components in parts by weight: 18-25 parts of grape seed oil, 11-15 parts of hawthorn seed oil and 2-3 parts of citric acid; wherein the average particle size of oil drops of the grape seed oil and the hawthorn seed oil is less than 150 nm. The antioxidant provided by the invention is mainly prepared from natural grape seed oil and hawthorn seed oil, and a small amount of citric acid is added, so that the three components are cooperated to be safe and healthy, and the antioxidant effect is good. The grape seed oil and the hawthorn seed oil are micronized, the average grain diameter of oil drops is less than 150nm, the viscosity is greatly reduced, the fluidity is enhanced, and the oil drops are more beneficial to contact with cottonseed oil, so that the grape seed oil and the hawthorn seed oil are uniformly mixed, and a better antioxidant effect is achieved. The citric acid is added, so that the dissolution of the grape seed oil and the hawthorn seed oil in the cottonseed oil can be promoted, and the antioxidation of the grape seed oil and the hawthorn seed oil can be fully exerted.
Description
Technical Field
The invention relates to an antioxidant for cottonseed oil. Belongs to the technical field of cottonseed oil.
Background
Food antioxidants are food additives that prevent or retard the oxidative deterioration of food, improve the stability of food, and prolong shelf life. The oxidation not only can deteriorate the grease in the food, but also can fade, discolor and destroy vitamins and the like of the food, thereby reducing the sensory quality and the nutritional value of the food, and even generating harmful substances to cause food poisoning.
Like all foods, the edible oil also needs to be preserved, and the main components of the edible oil are glycerin and fatty acid, which are easily oxidized to generate rancidity phenomenon, influence the taste, destroy the nutritional value and generate harmful components for human bodies. After the antioxidant is added, the oxidation of the grease can be delayed, the rancidity phenomenon is avoided, and the edible oil can be stored for a long time.
The antioxidant of the edible oil has two basic requirements, namely, good antioxidant effect is required, and other properties of the edible oil cannot be influenced. The conventional antioxidants commonly used in edible oils include TBHQ (tert-butylhydroquinone), BHA (butylhydroxyanisole), BHT (2, 6-di-tert-butyl-4-methylphenol), and the like, which are synthetic antioxidants.
The cottonseed oil has the unsaturated fatty acid content of about 75%, and the unsaturated fatty acid is shown to reduce the risk of cardiovascular and cerebrovascular diseases such as coronary heart disease in some human body dietary experiments relative to the saturated fatty acid, but the molecular structure of the unsaturated fatty acid contains one or more double bonds, so that the unsaturated fatty acid is unstable in chemical properties and easy to oxidize. The oil with serious oxidation has the phenomena of deepening color, increasing acid value and peroxide value, generating unpleasant odor and the like.
The aforementioned synthetic antioxidants are also widely used in cottonseed oil and are generally safe and reliable for use within the nationally regulated ranges and dosages. However, as the living standard and health consciousness of people improve, more and more people tend to eat natural foods rather than artificially synthesized foods. Moreover, in recent years, with the development of medical science, the safety of some synthetic antioxidants has been questioned, such as TBHQ has been banned in japan and BHT has also been banned in the united states. The prior artificially synthesized antioxidant has the problems of toxicity and safety, the antioxidant effect is not ideal, and especially the improvement space of the antioxidant effect after unsealing is limited.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide an antioxidant for cottonseed oil.
In order to achieve the purpose, the invention adopts the following technical scheme:
an antioxidant for cottonseed oil is prepared from the following components in parts by weight: 18-25 parts of grape seed oil, 11-15 parts of hawthorn seed oil and 2-3 parts of citric acid; wherein the average particle size of oil drops of the grape seed oil and the hawthorn seed oil is less than 150 nm.
Preferably, the feed is prepared from the following components in parts by weight: 20-22 parts of grape seed oil, 12-14 parts of hawthorn seed oil and 2-3 parts of citric acid.
Further preferably, the composition is prepared from the following components in parts by weight: 21 parts of grape seed oil, 13 parts of hawthorn seed oil and 3 parts of citric acid.
Even more preferably, the citric acid has an average particle size of less than 200 nm.
The grape seed oil is extracted by the following method: pulverizing grape seed, sieving with 100 mesh sieve, extracting with petroleum ether as extraction solvent, mixing petroleum ether and grape seed powder uniformly, extracting with 500W ultrasonic wave at 40 deg.C for 50 min, filtering, collecting filtrate, removing petroleum ether by rotary evaporation, and micronizing.
The mass volume ratio of the grape seed powder to the petroleum ether is 1 g: 12 mL.
Preferably, the micronizing treatment is performed by using an ultrahigh pressure homogenizer or a nano collider.
The hawthorn seed oil is extracted by the following method: pulverizing semen crataegi, sieving with 100 mesh sieve, extracting with petroleum ether as extraction solvent, mixing petroleum ether and semen crataegi oil uniformly, extracting with ultrasonic wave at 40 deg.C and 200W for 30 min, filtering, collecting filtrate, removing petroleum ether by rotary evaporation, and micronizing.
The mass volume ratio of the hawthorn seed powder to the petroleum ether is 1 g: 10 mL.
Preferably, the micronizing treatment is performed by using an ultrahigh pressure homogenizer or a nano collider.
The preparation method of the antioxidant for cottonseed oil comprises the following specific steps: adding citric acid into grape seed oil, stirring for dissolving, adding hawthorn seed oil, and stirring for mixing.
The use method of the antioxidant for cottonseed oil is that the antioxidant is added into cottonseed oil and stirred evenly; wherein the mass-to-volume ratio of the antioxidant to the cottonseed oil is 0.01 g: 100 mL.
The invention has the beneficial effects that:
the antioxidant provided by the invention is mainly prepared from natural grape seed oil and hawthorn seed oil, and a small amount of citric acid is added, so that the three components are cooperated to be safe and healthy, and the antioxidant effect is good. The antioxidant effect of the composition can be mainly derived from polyphenol compounds contained in grape seed oil and hawthorn seed oil. The polyphenol compound is a general term for plant components with a plurality of phenolic hydroxyl groups in the molecular structure, comprises phenolic acid and flavonoid compounds, is an excellent donor of hydrogen or electrons, and has obvious scavenging effect on superoxide anions and hydroxyl radical energy free radicals which can generate peroxidation to cause structural and functional damage. Phenolic compounds are excellent hydrogen proton or electron donors and can form stable phenolic radical intermediates, converting peroxides or other radicals into stable hydroperoxides, thereby preventing chain reactions from occurring, i.e., chain inhibiting providers. And because of the resonance delocalization effect and no position suitable for molecular oxygen attack, the material can not initiate new free radicals or be quickly oxidized due to chain reaction, and is relatively stable. It shows excellent antioxidant activity due to its high reactivity of hydrogen substitution and its ability to phagocytose free radicals.
The grape seed oil and the hawthorn seed oil are micronized, the average grain diameter of oil drops is less than 150nm, the viscosity is greatly reduced, the fluidity is enhanced, and the oil drops are more beneficial to contact with cottonseed oil, so that the grape seed oil and the hawthorn seed oil are uniformly mixed, and a better antioxidant effect is achieved. However, the grape seed oil and the hawthorn seed oil after the micronization treatment have extremely large specific surface area and relatively high specific surface energy and are in an extremely thermodynamically unstable state, and particles are easily coagulated and agglomerated in the subsequent treatment process to form secondary particles, so that the particle size of the particles is increased, and finally the specific functions of the micronized oil drops are lost in use. According to the invention, citric acid is added, so that on one hand, the citric acid can be combined with trace heavy metals in a complexing manner, and on the other hand, the citric acid can promote the dissolution of the grape seed oil and the hawthorn seed oil in the cottonseed oil (possibly playing a role similar to a stabilizer, inhibiting the agglomeration of oil drops of the grape seed oil and the hawthorn seed oil, and keeping the oil drops in a micronized state), so that the grape seed oil and the hawthorn seed oil can fully exert the antioxidation effect.
Detailed Description
The present invention will be further illustrated by the following examples, which are intended to be merely illustrative and not limitative.
Example 1:
the antioxidant for cottonseed oil is prepared from the following components: 18kg of grape seed oil, 11kg of hawthorn seed oil and 2kg of citric acid; wherein the average particle size of oil drops of the grape seed oil and the hawthorn seed oil is less than 150 nm.
The grape seed oil is extracted by the following method: crushing grape seeds, sieving with a 100-mesh sieve, taking petroleum ether as an extraction solvent, uniformly mixing the petroleum ether and grape seed powder, performing ultrasonic extraction at 40 ℃ and 500W for 50 minutes, filtering, taking filtrate, removing the petroleum ether by rotary evaporation, and performing micronization treatment by adopting an ultrahigh-pressure homogenizer or a nano collider. The mass volume ratio of the grape seed powder to the petroleum ether is 1 g: 12 mL.
The hawthorn seed oil is extracted by the following method: pulverizing semen crataegi, sieving with 100 mesh sieve, taking petroleum ether as extraction solvent, mixing petroleum ether and semen crataegi oil uniformly, extracting with ultrasonic wave at 40 deg.C and 200W for 30 min, filtering, collecting filtrate, removing petroleum ether by rotary evaporation, and micronizing with ultra-high pressure homogenizer or nanometer collider. The mass volume ratio of the hawthorn seed powder to the petroleum ether is 1 g: 10 mL.
The preparation method of the antioxidant for cottonseed oil comprises the following specific steps: adding citric acid into grape seed oil, stirring for dissolving, adding hawthorn seed oil, and stirring for mixing.
The use method of the antioxidant for cottonseed oil is that the antioxidant is added into cottonseed oil and stirred evenly; wherein the mass-to-volume ratio of the antioxidant to the cottonseed oil is 0.01 g: 100 mL.
Example 2:
the antioxidant for cottonseed oil is prepared from the following components: 25kg of grape seed oil, 15kg of hawthorn seed oil and 3kg of citric acid; wherein the average particle size of oil drops of the grape seed oil and the hawthorn seed oil is less than 150 nm.
The grape seed oil is extracted by the following method: crushing grape seeds, sieving with a 100-mesh sieve, taking petroleum ether as an extraction solvent, uniformly mixing the petroleum ether and grape seed powder, performing ultrasonic extraction at 40 ℃ and 500W for 50 minutes, filtering, taking filtrate, removing the petroleum ether by rotary evaporation, and performing micronization treatment by adopting an ultrahigh-pressure homogenizer or a nano collider. The mass volume ratio of the grape seed powder to the petroleum ether is 1 g: 12 mL.
The hawthorn seed oil is extracted by the following method: pulverizing semen crataegi, sieving with 100 mesh sieve, taking petroleum ether as extraction solvent, mixing petroleum ether and semen crataegi oil uniformly, extracting with ultrasonic wave at 40 deg.C and 200W for 30 min, filtering, collecting filtrate, removing petroleum ether by rotary evaporation, and micronizing with ultra-high pressure homogenizer or nanometer collider. The mass volume ratio of the hawthorn seed powder to the petroleum ether is 1 g: 10 mL.
The preparation method of the antioxidant for cottonseed oil comprises the following specific steps: adding citric acid into grape seed oil, stirring for dissolving, adding hawthorn seed oil, and stirring for mixing.
The use method of the antioxidant for cottonseed oil is that the antioxidant is added into cottonseed oil and stirred evenly; wherein the mass-to-volume ratio of the antioxidant to the cottonseed oil is 0.01 g: 100 mL.
Example 3:
the antioxidant for cottonseed oil is prepared from the following components: 20kg of grape seed oil, 12kg of hawthorn seed oil and 2kg of citric acid; wherein the average particle size of oil drops of the grape seed oil and the hawthorn seed oil is less than 150 nm.
The grape seed oil is extracted by the following method: crushing grape seeds, sieving with a 100-mesh sieve, taking petroleum ether as an extraction solvent, uniformly mixing the petroleum ether and grape seed powder, performing ultrasonic extraction at 40 ℃ and 500W for 50 minutes, filtering, taking filtrate, removing the petroleum ether by rotary evaporation, and performing micronization treatment by adopting an ultrahigh-pressure homogenizer or a nano collider. The mass volume ratio of the grape seed powder to the petroleum ether is 1 g: 12 mL.
The hawthorn seed oil is extracted by the following method: pulverizing semen crataegi, sieving with 100 mesh sieve, taking petroleum ether as extraction solvent, mixing petroleum ether and semen crataegi oil uniformly, extracting with ultrasonic wave at 40 deg.C and 200W for 30 min, filtering, collecting filtrate, removing petroleum ether by rotary evaporation, and micronizing with ultra-high pressure homogenizer or nanometer collider. The mass volume ratio of the hawthorn seed powder to the petroleum ether is 1 g: 10 mL.
The preparation method of the antioxidant for cottonseed oil comprises the following specific steps: adding citric acid into grape seed oil, stirring for dissolving, adding hawthorn seed oil, and stirring for mixing.
The use method of the antioxidant for cottonseed oil is that the antioxidant is added into cottonseed oil and stirred evenly; wherein the mass-to-volume ratio of the antioxidant to the cottonseed oil is 0.01 g: 100 mL.
Example 4:
the antioxidant for cottonseed oil is prepared from the following components: 22kg of grape seed oil, 14kg of hawthorn seed oil and 3kg of citric acid; wherein the average particle size of oil drops of the grape seed oil and the hawthorn seed oil is less than 150 nm.
The grape seed oil is extracted by the following method: crushing grape seeds, sieving with a 100-mesh sieve, taking petroleum ether as an extraction solvent, uniformly mixing the petroleum ether and grape seed powder, performing ultrasonic extraction at 40 ℃ and 500W for 50 minutes, filtering, taking filtrate, removing the petroleum ether by rotary evaporation, and performing micronization treatment by adopting an ultrahigh-pressure homogenizer or a nano collider. The mass volume ratio of the grape seed powder to the petroleum ether is 1 g: 12 mL.
The hawthorn seed oil is extracted by the following method: pulverizing semen crataegi, sieving with 100 mesh sieve, taking petroleum ether as extraction solvent, mixing petroleum ether and semen crataegi oil uniformly, extracting with ultrasonic wave at 40 deg.C and 200W for 30 min, filtering, collecting filtrate, removing petroleum ether by rotary evaporation, and micronizing with ultra-high pressure homogenizer or nanometer collider. The mass volume ratio of the hawthorn seed powder to the petroleum ether is 1 g: 10 mL.
The preparation method of the antioxidant for cottonseed oil comprises the following specific steps: adding citric acid into grape seed oil, stirring for dissolving, adding hawthorn seed oil, and stirring for mixing.
The use method of the antioxidant for cottonseed oil is that the antioxidant is added into cottonseed oil and stirred evenly; wherein the mass-to-volume ratio of the antioxidant to the cottonseed oil is 0.01 g: 100 mL.
Example 5:
the antioxidant for cottonseed oil is prepared from the following components: 21kg of grape seed oil, 13kg of hawthorn seed oil and 3kg of citric acid; wherein the average particle size of oil drops of the grape seed oil and the hawthorn seed oil is less than 150 nm.
The grape seed oil is extracted by the following method: crushing grape seeds, sieving with a 100-mesh sieve, taking petroleum ether as an extraction solvent, uniformly mixing the petroleum ether and grape seed powder, performing ultrasonic extraction at 40 ℃ and 500W for 50 minutes, filtering, taking filtrate, removing the petroleum ether by rotary evaporation, and performing micronization treatment by adopting an ultrahigh-pressure homogenizer or a nano collider. The mass volume ratio of the grape seed powder to the petroleum ether is 1 g: 12 mL.
The hawthorn seed oil is extracted by the following method: pulverizing semen crataegi, sieving with 100 mesh sieve, taking petroleum ether as extraction solvent, mixing petroleum ether and semen crataegi oil uniformly, extracting with ultrasonic wave at 40 deg.C and 200W for 30 min, filtering, collecting filtrate, removing petroleum ether by rotary evaporation, and micronizing with ultra-high pressure homogenizer or nanometer collider. The mass volume ratio of the hawthorn seed powder to the petroleum ether is 1 g: 10 mL.
The preparation method of the antioxidant for cottonseed oil comprises the following specific steps: adding citric acid into grape seed oil, stirring for dissolving, adding hawthorn seed oil, and stirring for mixing.
The use method of the antioxidant for cottonseed oil is that the antioxidant is added into cottonseed oil and stirred evenly; wherein the mass-to-volume ratio of the antioxidant to the cottonseed oil is 0.01 g: 100 mL.
Example 6:
the antioxidant for cottonseed oil is prepared from the following components: 21kg of grape seed oil, 13kg of hawthorn seed oil and 3kg of citric acid; wherein the average particle size of oil drops of the grape seed oil and the hawthorn seed oil is less than 150nm, and the average particle size of the citric acid is less than 200 nm.
The grape seed oil is extracted by the following method: crushing grape seeds, sieving with a 100-mesh sieve, taking petroleum ether as an extraction solvent, uniformly mixing the petroleum ether and grape seed powder, performing ultrasonic extraction at 40 ℃ and 500W for 50 minutes, filtering, taking filtrate, removing the petroleum ether by rotary evaporation, and performing micronization treatment by adopting an ultrahigh-pressure homogenizer or a nano collider. The mass volume ratio of the grape seed powder to the petroleum ether is 1 g: 12 mL.
The hawthorn seed oil is extracted by the following method: pulverizing semen crataegi, sieving with 100 mesh sieve, taking petroleum ether as extraction solvent, mixing petroleum ether and semen crataegi oil uniformly, extracting with ultrasonic wave at 40 deg.C and 200W for 30 min, filtering, collecting filtrate, removing petroleum ether by rotary evaporation, and micronizing with ultra-high pressure homogenizer or nanometer collider. The mass volume ratio of the hawthorn seed powder to the petroleum ether is 1 g: 10 mL.
The preparation method of the antioxidant for cottonseed oil comprises the following specific steps: adding citric acid into grape seed oil, stirring for dissolving, adding hawthorn seed oil, and stirring for mixing.
The use method of the antioxidant for cottonseed oil is that the antioxidant is added into cottonseed oil and stirred evenly; wherein the mass-to-volume ratio of the antioxidant to the cottonseed oil is 0.01 g: 100 mL.
Comparative example 1:
an antioxidant for cottonseed oil is prepared from the following components: 21kg of grape seed oil, 13kg of hawthorn seed oil and 3kg of citric acid.
The grape seed oil is extracted by the following method: crushing grape seeds, sieving with a 100-mesh sieve, taking petroleum ether as an extraction solvent, uniformly mixing the petroleum ether and the grape seed powder, performing ultrasonic extraction at 40 ℃ and 500W for 50 minutes, filtering, taking filtrate, and performing rotary evaporation to remove the petroleum ether to obtain the grape seed powder. The mass volume ratio of the grape seed powder to the petroleum ether is 1 g: 12 mL.
The hawthorn seed oil is extracted by the following method: pulverizing semen crataegi, sieving with 100 mesh sieve, extracting with petroleum ether as extraction solvent, mixing petroleum ether and semen crataegi oil uniformly, extracting with ultrasonic wave at 40 deg.C and 200W for 30 min, filtering, collecting filtrate, and removing petroleum ether by rotary evaporation. The mass volume ratio of the hawthorn seed powder to the petroleum ether is 1 g: 10 mL.
The preparation method of the antioxidant for cottonseed oil comprises the following specific steps: adding citric acid into grape seed oil, stirring for dissolving, adding hawthorn seed oil, and stirring for mixing.
The use method of the antioxidant for cottonseed oil is that the antioxidant is added into cottonseed oil and stirred evenly; wherein the mass-to-volume ratio of the antioxidant to the cottonseed oil is 0.01 g: 100 mL.
Comparative example 2:
an antioxidant for cottonseed oil is prepared from the following components: 21kg of grape seed oil and 13kg of hawthorn seed oil; wherein the average particle size of oil drops of the grape seed oil and the hawthorn seed oil is less than 150 nm.
The grape seed oil is extracted by the following method: crushing grape seeds, sieving with a 100-mesh sieve, taking petroleum ether as an extraction solvent, uniformly mixing the petroleum ether and grape seed powder, performing ultrasonic extraction at 40 ℃ and 500W for 50 minutes, filtering, taking filtrate, removing the petroleum ether by rotary evaporation, and performing micronization treatment by adopting an ultrahigh-pressure homogenizer or a nano collider. The mass volume ratio of the grape seed powder to the petroleum ether is 1 g: 12 mL.
The hawthorn seed oil is extracted by the following method: pulverizing semen crataegi, sieving with 100 mesh sieve, taking petroleum ether as extraction solvent, mixing petroleum ether and semen crataegi oil uniformly, extracting with ultrasonic wave at 40 deg.C and 200W for 30 min, filtering, collecting filtrate, removing petroleum ether by rotary evaporation, and micronizing with ultra-high pressure homogenizer or nanometer collider. The mass volume ratio of the hawthorn seed powder to the petroleum ether is 1 g: 10 mL.
The preparation method of the antioxidant for cottonseed oil comprises the following specific steps: mixing the citric acid and the hawthorn seed oil according to the formula amount, and uniformly stirring to obtain the hawthorn seed oil.
The use method of the antioxidant for cottonseed oil is that the antioxidant is added into cottonseed oil and stirred evenly; wherein the mass-to-volume ratio of the antioxidant to the cottonseed oil is 0.01 g: 100 mL.
Comparative example 3:
the grape seed oil is used as an antioxidant of cottonseed oil, the average particle size of oil drops is less than 150nm, and the mass-volume ratio of the grape seed oil to the cottonseed oil is 0.01 g: 100 mL.
The grape seed oil is extracted by the following method: crushing grape seeds, sieving with a 100-mesh sieve, taking petroleum ether as an extraction solvent, uniformly mixing the petroleum ether and grape seed powder, performing ultrasonic extraction at 40 ℃ and 500W for 50 minutes, filtering, taking filtrate, removing the petroleum ether by rotary evaporation, and performing micronization treatment by adopting an ultrahigh-pressure homogenizer or a nano collider. The mass volume ratio of the grape seed powder to the petroleum ether is 1 g: 12 mL.
Comparative example 4:
the hawthorn seed oil is used as an antioxidant of cottonseed oil, the average particle size of oil drops is less than 150nm, and the mass-volume ratio of the grape seed oil to the cottonseed oil is 0.01 g: 100 mL.
The hawthorn seed oil is extracted by the following method: pulverizing semen crataegi, sieving with 100 mesh sieve, taking petroleum ether as extraction solvent, mixing petroleum ether and semen crataegi oil uniformly, extracting with ultrasonic wave at 40 deg.C and 200W for 30 min, filtering, collecting filtrate, removing petroleum ether by rotary evaporation, and micronizing with ultra-high pressure homogenizer or nanometer collider. The mass volume ratio of the hawthorn seed powder to the petroleum ether is 1 g: 10 mL.
Test examples
The DPPH (1, 1-diphenyl-2-trinitrophenylhydrazine) method is used for measuring the oxidation resistance: diluting DPPH with methanol to make the concentration of DPPH 120 mu mol/L for later use; the antioxidants of examples 1 to 6 and comparative examples 1 to 4 were diluted with absolute ethanol respectively so that the mass ratio of the antioxidant to the absolute ethanol was 0.01 g: 100 mL.
Blank group: 2.0mL of absolute ethanol +2.0mL of 120. mu. mol/L DPPH methanol solution;
experimental groups: 2.0mL of ethanol solution prepared from the antioxidants of examples 1-6 and comparative examples 1-4 +2.0mL of 120. mu. mol/L DPPH methanol solution (two samples were taken randomly for comparative examples 1 and 2);
control group for each experimental group: 2.0mL of the antioxidants of examples 1-6 and comparative examples 1-4 +2.0mL of methanol (two samples were taken randomly for comparative example 1 and comparative example 2).
After the blank group, experimental group and control group reacted for 30 minutes, the color was compared at 517nm, and DPPH radical clearance rate was calculated according to the formula (DPPH radical clearance rate (C)/% (1- (a) /)1-A2)/A0) X 100, wherein C is DPPH radical clearance; a. the1The absorbance of each experimental group, namely the absorbance of a sample after removing DPPH free radicals; a. the2The absorbance of the control group reflects the contribution of the sample to the absorbance; a. the0Absorbance of the blank set, the absorbance of the DPPH radical itself at a specific wavelength. The test results are shown in Table 1.
TABLE 1 comparison of DPPH radical scavenging rates of antioxidants of examples 1-6 and comparative examples 1-4
| DPPH radical scavenging ratio (%) | |
| Example 1 | 92 |
| Example 2 | 92 |
| Example 3 | 94 |
| Example 4 | 93 |
| Example 5 | 95 |
| Example 6 | 96 |
| Comparative example 1 sample 1 | 70 |
| Comparative example 1 sample 2 | 55 |
| Comparative example 2 sample 1 | 60 |
| Comparative example 2 sample 2 | 75 |
| Comparative example 3 | 80 |
| Comparative example 4 | 73 |
As can be seen from Table 1, the antioxidants of examples 1-6 have higher DPPH radical scavenging rate, which is far better than that of grape seed oil (comparative example 3) or hawthorn seed oil (comparative example 4) used alone. Comparative example 1 two samples were taken at random, the difference in DPPH free radical scavenging rate was large, indicating that the antioxidant was not uniformly distributed in cottonseed oil and that the antioxidant effect was significantly affected, indicating that the addition of citric acid promoted the uniform mixing of antioxidant and cottonseed oil. Comparative example 2 also has similar data performance, indicating that the average oil droplet size directly affects the uniform distribution of the resulting antioxidant in the cottonseed oil.
Although the present invention has been described with reference to the specific embodiments, it is not intended to limit the scope of the present invention, and various modifications and variations can be made by those skilled in the art without inventive changes based on the technical solution of the present invention.
Claims (8)
1. The antioxidant for cottonseed oil is characterized by being prepared from the following components in parts by weight: 18-25 parts of grape seed oil, 11-15 parts of hawthorn seed oil and 2-3 parts of citric acid; wherein the average grain diameter of oil drops of the grape seed oil and the hawthorn seed oil is less than 150 nm; the average grain diameter of the citric acid is less than 200 nm;
the preparation method comprises the following steps: adding citric acid into grape seed oil, stirring for dissolving, adding hawthorn seed oil, and stirring for mixing.
2. The antioxidant for cottonseed oil according to claim 1, which is prepared from the following components in parts by weight: 20-22 parts of grape seed oil, 12-14 parts of hawthorn seed oil and 2-3 parts of citric acid.
3. The antioxidant for cottonseed oil according to claim 1, which is prepared from the following components in parts by weight: 21 parts of grape seed oil, 13 parts of hawthorn seed oil and 3 parts of citric acid.
4. The antioxidant for cottonseed oil according to any one of claims 1 to 3, wherein the grapeseed oil is extracted by the following method: pulverizing grape seed, sieving with 100 mesh sieve, extracting with petroleum ether as extraction solvent, mixing petroleum ether and grape seed powder uniformly, extracting with 500W ultrasonic wave at 40 deg.C for 50 min, filtering, collecting filtrate, removing petroleum ether by rotary evaporation, and micronizing.
5. The antioxidant for cottonseed oil according to claim 4, wherein the mass volume ratio of the grape seed powder to the petroleum ether is 1 g: 12 mL.
6. The antioxidant for cottonseed oil according to any one of claims 1 to 3, wherein the hawthorn seed oil is extracted by the following method: pulverizing semen crataegi, sieving with 100 mesh sieve, extracting with petroleum ether as extraction solvent, mixing petroleum ether and semen crataegi powder uniformly, extracting with ultrasonic wave at 40 deg.C and 200W for 30 min, filtering, collecting filtrate, removing petroleum ether by rotary evaporation, and micronizing.
7. The antioxidant for cottonseed oil according to claim 6, wherein the mass volume ratio of hawthorn seed powder to petroleum ether is 1 g: 10 mL.
8. The use method of the antioxidant for cottonseed oil according to any one of claims 1 to 3, wherein the antioxidant is obtained by adding the antioxidant into cottonseed oil, stirring and mixing; wherein the mass-to-volume ratio of the antioxidant to the cottonseed oil is 0.01 g: 100 mL.
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