CN106093110A - A kind of automatic measuring thermal for measuring microalgae growth curve - Google Patents
A kind of automatic measuring thermal for measuring microalgae growth curve Download PDFInfo
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- CN106093110A CN106093110A CN201610403227.XA CN201610403227A CN106093110A CN 106093110 A CN106093110 A CN 106093110A CN 201610403227 A CN201610403227 A CN 201610403227A CN 106093110 A CN106093110 A CN 106093110A
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- calorstat
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- measuring thermal
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N25/00—Investigating or analyzing materials by the use of thermal means
- G01N25/20—Investigating or analyzing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity
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- Investigating Or Analyzing Materials Using Thermal Means (AREA)
Abstract
A kind of automatic measuring thermal for measuring microalgae growth curve belongs to automatic measuring thermal technology field, this automatic measuring thermal includes Calorimetry system and computer control system, LED light source is added, it is provided that the condition needed for microalgae growth, it is ensured that the verity of experiment in the ampoule bottle of monitoring sample；Under the protection of calorstat, create a metastable test environment, it is to avoid the loss of heat during monitoring, and variation of ambient temperature grows brought unfavorable factor to microalgae, substantially increases the degree of accuracy of mensuration microalgae growth curve heat effect；The low cost of the present invention, certainty of measurement is high, and simple in construction, easy to operate.
Description
Technical field
The invention belongs to automatic measuring thermal technology field, particularly relate to a kind of automatic measuring for measuring microalgae growth curve
Thermal.
Background technology
Calorimetric Techniques is important Physical Chemistry Experiment method and experimental technique, for the assertive evidence energy of measurement of species, logical
Cross the thermal change data that measure in course of reaction can reacting substance energy variation before and after physical-chemical reaction, and calculate
Go out corresponding thermodynamic parameter, and then deduce the thermodynamic state of this system.
Intelligent sensor technology be one just in flourish Modern Transducer Technology, it relates to micromechanics and microelectronics skill
Art, signal processing technology, computer technology, Circuits and Systems, nerual network technique, sensing technology and fuzzy control theory etc. are many
Plant subject, be an integrated technology.
Microalgae (micro-algae) is a kind of aquatic rudimentary plant that can effectively utilize luminous energy and carbon dioxide, its kind
Various, widely distributed, have that Biomass is big, growth cycle is short, be prone to cultivate and lipid content high, be considered by the world
The living resources of the most potential a kind of petroleum replacing, its pilot scale culture is to solve current energy source shortage and problem of environmental pollution
Effective means.
All life process is all along with heat effect.These heat effects can be followed the tracks of by calorimetry accurately, and characterizes
Its process.At present, calorimetry is widely used to the research fields such as pharmacology, biochemistry, cytology, but for aquatic microalgae
The research of growth curve heat effect but rarely has application.The work of current all calorimeters employed both at home and abroad or calorimeter assembly is former
Reason is all based on the static test of tradition calorimeter, and i.e. measured sample is the material prepared, including solid,
Gas or liquid, such as solid rubber, gases methane or liquid ethanol, put into set environment such as oxygen atmosphere exhausted by the material tested
The hermetic container of heat carries out heating or burning, then measures the heat that material is released or absorbed, and record in this course
Thermal change finally deduct heating electric power and be the heat that this material is released in this condition or absorbed from the heat of record
Amount, finally gives the thermodynamic data of tested material.Such as the patent No. 87212029, entitled Isothermal Automatic Calorimeter, though
So improving to some extent on tradition calorimeter, but its structure still suffers from deficiency, operation is complicated, strict to operating environment requirements, for
Microalgae Organism Samples cannot implement heat monitoring.The heat effect of the chemical reaction in organism is the least, if energy
Measure heat effect produced by biochemical reaction by a kind of method, just biosystem can be carried out static tracking and measuring.
Theoretical according to this, Microcalorimetric method hence sets up, and the ratio that the TA company of the current U.S. does in this field is more prominent, also
Have Germany C80calorimeter be all highly sophisticated device, but be all involve great expense, operate complexity, monitoring have locality, and
And current all of micro calorimeter is all airtight, there is no light source, be not suitable for the growth for monitoring microalgae.
Summary of the invention
The present invention is to solve the problem that prior art exists, it is provided that a kind of automatic measuring measuring microalgae growth curve
Thermal, heat effect produced in microalgae growth course can be monitored, and be provided microalgae by this device in real time
Conditions of existence needed for growth, does not cause any destruction to sample itself, under the support of multichannel recording instrument without paper, by temperature
The small on-the-spot thermal change Real-time Collection of sensor senses is in computer system, by the help of corresponding software, and can be quick
Comparison calculate, Dynamic Announce variation tendency on electronic curtain, and achieve the test merit such as data real-time storage, resource-sharing
Energy.It addition, under the protection of calorstat, create a metastable test environment, it is to avoid heat during monitoring
Run off, and variation of ambient temperature grows brought unfavorable factor to microalgae, substantially increase mensuration microalgae growth curve
The degree of accuracy of heat effect.
For achieving the above object, concrete technical scheme is as follows:
A kind of automatic measuring thermal for measuring microalgae growth curve, this automatic measuring thermal include Calorimetry system and
Computer control system, described Calorimetry system includes speed governing cyclotron oscillation device 3, calorstat 4, temperature probe 5, temperature controller 6, temperature
Degree sensor 7, ampoule bottle 8, LED battery lamp 9, low-pressure heating line 10；Described computer control system includes computer 1 and nothing
Paper monitor 2.
Described calorstat 4 is placed on the platform of speed governing cyclotron oscillation device 3, places ampoule bottle 8, LED battery in calorstat 4
Lamp 9 is fixed on the inwall of ampoule bottle 8 bottle mouth position.
Temperature probe 5 within calorstat 4 monitors constant temperature the temperature inside the box, and temperature probe 5 is passed by low-pressure heating line 10
The aperture at calorstat 4 top, is connected with temperature controller 6.
Low-pressure heating line 10 it is wound around, for heated constant temperature case on calorstat 4 inwall；Temperature controller 6 controls low-pressure heating line 10
Switch, maintains the temperature constant in calorstat.
The sound end of temperature sensor 7 is inserted in the testing sample in ampoule bottle 8, the other end of temperature sensor 7 with
Recording instrument without paper 2 is connected；Recording instrument without paper 2 is connected with computer 1.
Temperature sensor monitors to temperature change signal be transferred to recording instrument without paper, thus in computer control system
Show in the form of images.Recording instrument without paper includes 4 separate passages, can monitor simultaneously many kinds of parameters or
Same sample is implemented long-time on-line monitoring.
Beneficial effects of the present invention:
1) present invention uses temperature sensor to carry out the heat effect in tracking and monitoring microalgae growth course, with conventional amounts hot charging
Put and compare, both overcome a difficult problem for the long-time monitoring to biopsy samples, saved again the integrity of testing sample, and react
Device provides the primary condition needed for microalgae growth, it is ensured that the life that testing sample can be healthy during measuring
Long, it is provided that the verity of data and reliability.
2) Calorimetric Techniques is applied in the monitoring of microalgae growth curve by the present invention, and application amount thermal technology monitors microalgae
The growing state of class, provides foundation for understanding microalgae Ecological niche information.
3) comparing other calorimeter assemblies, the present invention adds LED light source first in the ampoule bottle of monitoring sample, it is provided that microalgae
Condition needed for class growth, it is ensured that the verity of experiment.
4) low cost of the present invention, certainty of measurement is high, and simple in construction, easy to operate.
Accompanying drawing explanation
Fig. 1 is the block diagram of automatic measuring thermal.
Fig. 2 a is the top view of polystyrene foam calorstat.
Fig. 2 b is the sectional view of polystyrene foam calorstat.
Fig. 3 is the structural representation of glass ampoule bottles.
Fig. 4 is the profile of glass ampoule bottles.
In figure:
1 computer；2 recording instrument without paper；3 speed governing cyclotron oscillation devices；4 calorstats；5 temperature probes；6 temperature controllers；7 temperature pass
Sensor；8 ampoule bottles；9LED battery lamp；10 low-pressure heating lines；11 caping；12 aluminum foil linings；13 internal layer silicagel pad；14 vials
Body；15 button cells；16LED lamp bead.
Detailed description of the invention
A kind of automatic measuring thermal for measuring microalgae growth curve, this automatic measuring thermal include Calorimetry system and
Computer control system, described Calorimetry system includes speed governing cyclotron oscillation device 3, calorstat 4, temperature probe 5, temperature controller 6, temperature
Degree sensor 7, ampoule bottle 8, LED battery lamp 9, low-pressure heating line 10；Described computer control system includes computer 1 and nothing
Paper monitor 2.
Described calorstat 4 is placed on the platform of speed governing cyclotron oscillation device 3, wall thickness 5cm in calorstat 4, and inwall is polyphenyl
Ethylene foam material, calorstat 4 bottom interior wall leaves the hole of 4 ampoule bottle 8 sizes, for placing 4 ampoule bottles 8.Peace
Small jar bottle 8 has volume to be tetra-kinds of different sizes of 5ml, 10ml, 15ml and 20ml, is respectively placed in different polystyrene foams, peace
Small jar bottle 8 is by glass body 14, internal layer silicagel pad 13, aluminum foil lining 12 and caping 11 4 part composition.LED battery lamp 9 wavelength model
Enclose 400-700nm, PUR be fixed on the inwall of ampoule bottle 8 bottle mouth position.
Temperature probe 5 within calorstat 4 monitors constant temperature the temperature inside the box, and temperature probe 5 is passed by low-pressure heating line 10
The aperture at calorstat 4 top, is connected with temperature controller 6.
Low-pressure heating line 10 it is wound around, for heated constant temperature case on calorstat 4 inwall；Temperature controller 6 controls low-pressure heating line 10
Switch, maintains the temperature constant in calorstat.
The sound end of temperature sensor 7 is inserted in the testing sample in ampoule bottle 8, and at the bottom of ampoule bottle 8 bottles, temperature passes
The other end of sensor 7 is connected with recording instrument without paper 2；Recording instrument without paper 2 is connected with computer 1.
Claims (10)
1. the automatic measuring thermal being used for measuring microalgae growth curve, it is characterised in that: this automatic measuring thermal includes
Calorimetry system and computer control system, described Calorimetry system includes that speed governing cyclotron oscillation device (3), calorstat (4), temperature are visited
Head (5), temperature controller (6), temperature sensor (7), ampoule bottle (8), LED battery lamp (9), low-pressure heating line (10)；Described meter
Calculation machine control system includes computer (1) and recording instrument without paper (2)；
Described calorstat (4) is placed on the platform of speed governing cyclotron oscillation device (3), places ampoule bottle (8), LED in calorstat (4)
Battery lamp (9) is fixed on the inwall of ampoule bottle (8) bottle mouth position；
Temperature probe (5) monitoring constant temperature the temperature inside the box that calorstat (4) is internal, temperature probe (5) passes through low-pressure heating line (10)
Through the aperture at calorstat (4) top, it is connected with temperature controller (6)；
Low-pressure heating line (10) it is wound around, for heated constant temperature case on calorstat (4) inwall；Temperature controller (6) controls low-pressure heating line
(10) switch, maintains the temperature constant in calorstat；
The sound end of temperature sensor (7) is inserted in the testing sample in ampoule bottle (8), the other end of temperature sensor (7)
It is connected with recording instrument without paper (2)；Recording instrument without paper (2) is connected with computer (1).
Automatic measuring thermal the most according to claim 1, it is characterised in that: described LED battery lamp wave-length coverage 400-
700nm。
Automatic measuring thermal the most according to claim 1 and 2, it is characterised in that: described LED battery lamp is solid by PUR
It is scheduled on the inwall of ampoule bottle (8) bottle mouth position.
Automatic measuring thermal the most according to claim 1 and 2, it is characterised in that: the sound end of temperature sensor (7) is higher than
At the bottom of ampoule bottle (8) bottle.
Automatic measuring thermal the most according to claim 3, it is characterised in that: the sound end of temperature sensor (7) is higher than peace
At the bottom of small jar bottle (8) bottle.
6. according to the automatic measuring thermal described in claim 1 or 2 or 5, it is characterised in that: stay in calorstat (4) bottom interior wall
There is the hole of 3-5 ampoule bottle (8) size, be used for placing ampoule bottle (8).
Automatic measuring thermal the most according to claim 3, it is characterised in that: leave 3-5 in calorstat (4) bottom interior wall
The hole of ampoule bottle (8) size, is used for placing ampoule bottle (8).
Automatic measuring thermal the most according to claim 4, it is characterised in that: leave 3-5 in calorstat (4) bottom interior wall
The hole of ampoule bottle (8) size, is used for placing ampoule bottle (8).
9. according to the automatic measuring thermal described in claim 1 or 2 or 5 or 7 or 8, it is characterised in that: in described calorstat (4)
Wall thickness 4-6cm, inwall is polystyrene foam.
Automatic measuring thermal the most according to claim 6, it is characterised in that: described calorstat (4) interior wall thickness 4-6cm, interior
Wall is polystyrene foam.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610403227.XA CN106093110A (en) | 2016-06-08 | 2016-06-08 | A kind of automatic measuring thermal for measuring microalgae growth curve |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610403227.XA CN106093110A (en) | 2016-06-08 | 2016-06-08 | A kind of automatic measuring thermal for measuring microalgae growth curve |
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| CN106093110A true CN106093110A (en) | 2016-11-09 |
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| CN201610403227.XA Pending CN106093110A (en) | 2016-06-08 | 2016-06-08 | A kind of automatic measuring thermal for measuring microalgae growth curve |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770438A (en) * | 2016-11-21 | 2017-05-31 | 大连理工大学 | A kind of assay method of microalgae growth curve and application |
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| CN101258401A (en) * | 2005-09-01 | 2008-09-03 | 精工电子纳米科技有限公司 | Heat flow flux type differential scanning calorimeter |
| CN102645448A (en) * | 2012-04-10 | 2012-08-22 | 西安科技大学 | Method for measuring growth cycle of puccinia striiformis by using microcalorimetric method |
| CN203274965U (en) * | 2013-06-07 | 2013-11-06 | 鹤壁市天信科技有限公司 | Four-barrel calorimeter |
| CN104807854A (en) * | 2009-07-07 | 2015-07-29 | 吴耿 | Heat system and operation method |
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2016
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Patent Citations (5)
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| CN101258401A (en) * | 2005-09-01 | 2008-09-03 | 精工电子纳米科技有限公司 | Heat flow flux type differential scanning calorimeter |
| WO2007101379A1 (en) * | 2006-03-07 | 2007-09-13 | Huawei Technologies Co., Ltd. | A method, router and system for multicast stream forwarding |
| CN104807854A (en) * | 2009-07-07 | 2015-07-29 | 吴耿 | Heat system and operation method |
| CN102645448A (en) * | 2012-04-10 | 2012-08-22 | 西安科技大学 | Method for measuring growth cycle of puccinia striiformis by using microcalorimetric method |
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| CN106770438A (en) * | 2016-11-21 | 2017-05-31 | 大连理工大学 | A kind of assay method of microalgae growth curve and application |
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Application publication date: 20161109 |
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