CN106083248A - A kind of method utilizing energy grass biogas residue ridge-up bed method culturing edible fungus - Google Patents

A kind of method utilizing energy grass biogas residue ridge-up bed method culturing edible fungus Download PDF

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Publication number
CN106083248A
CN106083248A CN201610414528.2A CN201610414528A CN106083248A CN 106083248 A CN106083248 A CN 106083248A CN 201610414528 A CN201610414528 A CN 201610414528A CN 106083248 A CN106083248 A CN 106083248A
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energy grass
grass
edible
biogas residue
energy
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刘斌
肖正
林满红
范锦琳
杨丽秋
刘欣怡
林占熺
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Fujian Agriculture and Forestry University
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Fujian Agriculture and Forestry University
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mushroom Cultivation (AREA)

Abstract

It is an object of the invention to utilize energy grass biogas residue and fresh energy grass to use ridge-up bed method culturing edible fungus for planting material, realize the efficient utilization of energy grass biogas residue and energy grass, reduce the discharge of energy grass biogas fermentation garbage, improve the economic benefit of biogas engineering, widen the raw material sources of edible fungi simultaneously.The present invention had both taken full advantage of the advantage of energy grass biogas residue height lignocellulose, take full advantage of again the feature of fresh energy grass rich in nutrition content, the more conventional raw material of productivity making edible fungi improves 20% ~ 30%, production cycle shortens 3 ~ 5 days, and the economic benefit of energy grass biogas engineering is greatly improved.Energy grass biogas residue and fresh energy grass raw material are sufficient, are beneficial to realize the large-scale production of edible fungi.

Description

A kind of method utilizing energy grass biogas residue ridge-up bed method culturing edible fungus
Technical field
The invention belongs to utilization of agricultural resources utilization field, relate to cultivating method for edible fungi, be specifically related to one and utilize energy grass Biogas residue uses the method for ridge-up bed method culturing edible fungus.
Background technology
Energy grass includes annual or perennial, high wood fibre cellulose content, high yield, the draft energy of high photosynthesis efficiency Plant, is the huge energy-source plant of potentiality to be exploited, it has also become one of primary raw material of biogas engineering.Energy grass biogas is sent out at present Biogas residue after ferment is directly discharged in environment, or simple development becomes fertilizer, and economic benefit is the highest.
Edible fungi is cultivated at present with Caulis et Folium Oryzae, waste cotton, cotton seed hulls, cotton skin, corn cob, wheat straw, rice husk, bean stalk powder, bagasse Being main Deng conventional agricultural raw material, these raw materials are because wood fibre cellulose content is low, raw material output is low, it is difficult to meet batch production, scale The demand that metaplasia is produced.
The comprehensive cyclic utilization method of a CN 103752588 A(energy grass) to relate to the cultivation of energy grass biogas residue edible The method of bacterium, but the method is only with energy grass biogas residue as primary raw material, and energy grass biogas residue nutritional labeling is not enough, it is difficult to significantly carry The productivity of high edible fungi.
Summary of the invention
It is an object of the invention to utilize energy grass biogas residue and fresh energy grass to use the cultivation of ridge-up bed method edible for planting material Bacterium, it is achieved energy grass biogas residue and the efficient utilization of energy grass, reduces the discharge of energy grass biogas fermentation garbage, improves biogas work The economic benefit of journey, widens the raw material sources of edible fungi simultaneously.
For achieving the above object, the present invention adopts the following technical scheme that
A kind of method utilizing energy grass biogas residue ridge-up bed method culturing edible fungus, comprises the following steps:
(1) trophophase of gathering is the fresh energy grass of 3 ~ 8 months, is directly crushed to 0.5 ~ 5 centimetre and obtains energy grass coarse powder, then will The energy grass coarse powder of 30 ~ 50% is dried to moisture 10 ~ 30wt%, then be crushed to less than 0.5 centimetre energy grass fine powder;
(2) by step (1) gained energy grass coarse powder after ensiling or not ensiling, biogas fermentation is carried out, 33 ~ 42 DEG C of fermentations 25 ~ Fermenting 12 ~ 16 days in 35 days or temperature 50 C ~ 58 DEG C, obtains biogas residue and biogas liquid mixture;
(3) the biogas residue and biogas liquid mixture obtained in step (2) is passed through solid-liquid separation, obtain energy grass biogas residue and energy marsh Liquid;
(4) the energy grass biogas residue that step (3) separates is dried to moisture content 60 ~ 85wt%, is piled into 1 ~ 3 meter of high stacking, middle Every 30 ~ 50 centimetres of jacks, pore size 3 ~ 8 centimetres, cover with plastic foil, open plastic sheeting every day 1 ~ 3 time, each 10 ~ 60 minutes, pile up 10 ~ 30 days time;
(5) the energy grass biogas slurry that step (3) separates is carried out aeration, by weight, by Sal 5 ~ 10 parts, glucose or sucrose 5 ~ 10 parts, 1 ~ 3 part of carbamide, potassium dihydrogen phosphate 0.2 ~ 0.8 part add in 1600 ~ 2000 parts of clear water, boil 30 ~ 60 minutes, after cooling Seal and preserve, add the biogas slurry 100 ~ 150 parts after aeration before using, regulate pH 6.8 ~ 8.5, obtain edible mushroom cultivation nutrition liquid;
(6) by weight, by step (4) through accumulation after energy grass biogas residue 35 ~ 55 parts, step (1) gained energy grass fine powder 40 ~ 65 parts, Testa Tritici or 0 ~ 16 part of Testa oryzae, Gypsum Fibrosum powder or 2.5 ~ 3 parts of mix homogeneously of pulverized limestone, the edible fungi prepared by step (5) Cultivating nutrient solution regulation water content 55 ~ 70wt%, is 6.0 ~ 9.2 with lime water regulation pH, obtains culture medium of edible fungus;
(7) step (6) gained culture medium of edible fungus is built heap fermentation, the sterilization of mushroom bed, make the bed sowing, earthing, routinely technology control Temperature processed, humidity and illumination, cultivate hypha of edible fungus and fruiting;
(8), after edible mushroom 4 ~ 7 days, when cap basically forms, plant to planting material surface sprinkling step (5) gained edible fungi Cultivation nutrient fluid, it is 0.2 ~ 0.4 kilogram that every square metre of planting material sprays nutritional solution consumption;
(9) fruit body of edible fungi growth, ripe, gather;
(10) after fruit body of edible fungi is gathered, every 4 ~ 8 days, next tide mushroom walks to culture medium of edible fungus surface sprinkling after growing again Suddenly the edible mushroom cultivation nutrition liquid that prepared by (5), every square metre of culture medium of edible fungus sprays nutritional solution 0.5 ~ 0.8 kilogram, uses clear water Regulation planting material water content is to 55 ~ 70wt%;
(11) the second tide mushroom can be obtained according to step (8) ~ (9), continue to repeat step (10), (8) and (9), the three to five tide can be obtained Mushroom.
Described energy grass is hybrid pennisetum, thin stem hybridization wolf tail, purple grassiness, dwarf elephant grass, red grassiness, grassiness, herbage Sugarcane, Jujun grasses, Pennisetum hydridum, Taiwan Herba Hedyotis cantonensis, Sorghum propinquum, sugar grass, Arundo donax, phragmites communis, Plantula Neyraudiae Reynaudianae, Radix sacchari arundinacei, those special grass, arabian cron, U.S.s Continent Herba penniseti, Gui Mu, Nan Mu, platform agriculture, Fujian are herded, imperatoria, Caulis Miscanthis floriduli, Miscanthus sacchariflorus (Maxim) Benth et Hook f, Dicranopteris amplachinget Chiu, Arundinella hirta (Thunb.) Tanaka, Plantula Neyraudiae Reynaudianae, silagecorn, perfume (or spice) One or more in thatch, Spartina alterniflora, Value of Spartina Anglica, switchgrass, S. spontaneum.
Described edible fungi includes in Agaricus bisporus, Volvariella volvacea (Bull.Ex Franch.) Singer., Coprinus comatus, Caulis Bambusae In Taeniam, Brazilian mushroom, Stropharia rugoso-annulata, Pleurotus ostreatus Kind.
The beneficial effects of the present invention is: the present invention had both taken full advantage of the advantage of energy grass biogas residue height lignocellulose, Taking full advantage of again the feature of fresh energy grass rich in nutrition content so that the more conventional raw material of productivity of edible fungi improves 20% ~ 30%, the production cycle shortens 3 ~ 5 days, and the economic benefit of energy grass biogas engineering is greatly improved.Energy grass biogas residue and fresh energy Grassland, source material abundance, is beneficial to realize the large-scale production of edible fungi.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be further described, but the present invention is not limited only to these embodiments.
Embodiment 1
A kind of method utilizing energy grass biogas residue ridge-up bed method culturing edible fungus, comprises the following steps:
(1) trophophase of gathering is the fresh energy grass of 3 months, is directly crushed to 0.5 centimetre and obtains energy grass coarse powder, then by 30% Energy grass coarse powder be dried to moisture 20wt%, then be crushed to 0.2 centimetre energy grass fine powder;
(2) by step (1) gained energy grass coarse powder after ensiling, carrying out biogas fermentation, 33 DEG C ferment 25 days, obtain biogas residue natural pond Liquid mixture;
(3) the biogas residue and biogas liquid mixture obtained in step (2) is passed through solid-liquid separation, obtain energy grass biogas residue and energy marsh Liquid;
(4) the energy grass biogas residue that step (3) separates is dried to moisture content 60wt%, is piled into 1 meter of high stacking, middle every 30 centimetres of jacks, pore size 3 centimetres, cover with plastic foil, open plastic sheeting every day 1 time, each 30 minutes, pile up the time 20 days;
(5) the energy grass biogas slurry that step (3) separates is carried out aeration, by weight, by Sal 5 parts, glucose 5 parts, carbamide 1 Part, potassium dihydrogen phosphate 0.2 part add in 1600 parts of clear water, boil 30 minutes, seal and preserve after cooling, before using after addition aeration Biogas slurry 100 parts, regulate pH 6.8, obtain cultivating nutrient solution;
(6) by weight, by step (4) through accumulation after energy grass biogas residue 35 parts, step (1) gained energy grass fine powder 40 Part, 5 parts of Testa Tritici, 2.5 parts of mix homogeneously of Gypsum Fibrosum powder, the edible mushroom cultivation nutrition liquid regulation water content prepared by step (5) 55wt%, is 6.0 with lime water regulation pH, obtains culture medium of edible fungus;
(7) step (6) gained culture medium of edible fungus is built heap fermentation, the sterilization of mushroom bed, make the bed sowing, earthing, routinely technology control Temperature processed, humidity and illumination, cultivate hypha of edible fungus and fruiting;
(8) after edible mushroom 4 days, when cap basically forms, to planting material surface sprinkling step (5) gained edible fungus culturing Nutritional solution, it is 0.2 kilogram that every square metre of planting material sprays nutritional solution consumption;
(9) fruit body of edible fungi growth, ripe, gather;
(10) after fruit body of edible fungi is gathered, every 4 days, next tide mushroom is made to planting material surface sprinkling step (5) after growing again Standby edible mushroom cultivation nutrition liquid, every square metre of planting material is sprayed nutritional solution 0.5 kilogram, is arrived by clear water regulation planting material water content 55wt%;
(11) the second tide mushroom can be obtained according to step (8) ~ (9), continue to repeat step (10), (8) and (9), the three to five tide can be obtained Mushroom.
Described energy grass is hybrid pennisetum.
Described edible fungi is Agaricus bisporus.
Embodiment 2
A kind of method utilizing energy grass biogas residue ridge-up bed method culturing edible fungus, comprises the following steps:
(1) trophophase of gathering is the fresh energy grass of 5 months, is directly crushed to 3 centimetres and obtains energy grass coarse powder, then by 40% Energy grass coarse powder is dried to moisture 15wt%, then be crushed to less than 0.5 centimetre energy grass fine powder;
(2) step (1) gained energy grass coarse powder carrying out biogas fermentation, 40 DEG C ferment 30 days, obtain biogas residue and biogas liquid mixture;
(3) the biogas residue and biogas liquid mixture obtained in step (2) is passed through solid-liquid separation, obtain energy grass biogas residue and energy marsh Liquid;
(4) the energy grass biogas residue that step (3) separates is dried to moisture content 70wt%, is piled into 2 meters of high stackings, middle every 40 centimetres of jacks, pore size 5 centimetres, cover with plastic foil, open plastic sheeting every day 2 times, each 60 minutes, pile up the time 25 days;
(5) the energy grass biogas slurry that step (3) separates is carried out aeration, by weight, by Sal 8 parts, sucrose 8 parts, 2 parts of carbamide, Potassium dihydrogen phosphate 0.6 part adds in 1800 parts of clear water, boils 45 minutes, seals and preserves, after adding aeration before using after cooling Biogas slurry 130 parts, regulates pH 8.0, obtains cultivating nutrient solution;
(6) by weight, by step (4) through accumulation after energy grass biogas residue 40 parts, step (1) gained energy grass fine powder 50 Part, 2.5 parts of mix homogeneously of pulverized limestone, edible mushroom cultivation nutrition liquid regulation water content 60wt% prepared by step (5), use Calx Water regulation pH is 7.0, obtains culture medium of edible fungus;
(7) step (6) gained culture medium of edible fungus is built heap fermentation, the sterilization of mushroom bed, make the bed sowing, earthing, routinely technology control Temperature processed, humidity and illumination, cultivate hypha of edible fungus and fruiting;
(8) after edible mushroom 6 days, when cap basically forms, to planting material surface sprinkling step (5) gained edible fungus culturing Nutritional solution, it is 0.3 kilogram that every square metre of planting material sprays nutritional solution consumption;
(9) fruit body of edible fungi growth, ripe, gather;
(10) after fruit body of edible fungi is gathered, every 5 days, next tide mushroom is made to planting material surface sprinkling step (5) after growing again Standby edible mushroom cultivation nutrition liquid, every square metre of planting material is sprayed nutritional solution 0.6 kilogram, is arrived by clear water regulation planting material water content 60wt%;
(11) the second tide mushroom can be obtained according to step (8) ~ (9), continue to repeat step (10), (8) and (9), the three to five tide can be obtained Mushroom.
Described energy grass is that thin stem hybridizes wolf tail.
Described edible fungi is Brazilian mushroom.
Embodiment 3
A kind of method utilizing energy grass biogas residue ridge-up bed method culturing edible fungus, comprises the following steps:
(1) trophophase of gathering is the fresh energy grass of 8 months, is directly crushed to 5 centimetres and obtains energy grass coarse powder, then by 50% Energy grass coarse powder is dried to moisture 10wt%, then be crushed to less than 0.5 centimetre energy grass fine powder;
(2) by step (1) gained energy grass coarse powder after ensiling, carrying out biogas fermentation, 52 DEG C ferment 16 days, obtain biogas residue Biogas slurry mixture;
(3) the biogas residue and biogas liquid mixture obtained in step (2) is passed through solid-liquid separation, obtain energy grass biogas residue and energy marsh Liquid;
(4) the energy grass biogas residue that step (3) separates is dried to moisture content 85wt%, is piled into 3 meters of high stackings, middle every 50 centimetres of jacks, pore size 8 centimetres, cover with plastic foil, open plastic sheeting every day 3 times, each 60 minutes, pile up the time 30 days;
(5) energy grass biogas slurry high pressure Kalvar process step (3) separated carries out aeration, by weight, by Sal 10 parts, sucrose 10 parts, 3 parts of carbamide, potassium dihydrogen phosphate 0.8 part add in 2000 parts of clear water, boil 60 minutes, seal after cooling and preserve, before using Add the biogas slurry 150 parts after aeration, regulate pH 8.5, obtain cultivating nutrient solution;
(6) by weight, by step (4) through accumulation after energy grass biogas residue 55 parts, step (1) gained energy grass fine powder 65 Part, 16 parts of Testa Tritici, 3 parts of mix homogeneously of pulverized limestone, edible mushroom cultivation nutrition liquid regulation water content 70wt% prepared by step (5), It is 9.2 with lime water regulation pH, obtains culture medium of edible fungus;
(7) step (6) gained culture medium of edible fungus is built heap fermentation, the sterilization of mushroom bed, make the bed sowing, earthing, routinely technology control Temperature processed, humidity and illumination, cultivate hypha of edible fungus and fruiting;
(8) after edible mushroom 7 days, when cap basically forms, to planting material surface sprinkling step (5) gained edible fungus culturing Nutritional solution, it is 0.4 kilogram that every square metre of planting material sprays nutritional solution consumption;
(9) fruit body of edible fungi growth, ripe, gather;
(10) after fruit body of edible fungi is gathered, every 8 days, next tide mushroom is made to planting material surface sprinkling step (5) after growing again Standby edible mushroom cultivation nutrition liquid, every square metre of planting material is sprayed nutritional solution 0.8 kilogram, is arrived by clear water regulation planting material water content 70wt%;
(11) the second tide mushroom can be obtained according to step (8) ~ (9), continue to repeat step (10), (8) and (9), the three to five tide can be obtained Mushroom.
Described energy grass is sugar grass.
Described edible fungi is Coprinus comatus.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with Modify, all should belong to the covering scope of the present invention.

Claims (3)

1. the method utilizing energy grass biogas residue ridge-up bed method culturing edible fungus, it is characterised in that: comprise the following steps:
(1) trophophase of gathering is the fresh energy grass of 3 ~ 8 months, is directly crushed to 0.5 ~ 5 centimetre and obtains energy grass coarse powder, then will The energy grass coarse powder of 30 ~ 50% is dried to moisture 10 ~ 30wt%, then be crushed to less than 0.5 centimetre energy grass fine powder;
(2) by step (1) gained energy grass coarse powder after ensiling or not ensiling, biogas fermentation is carried out, 33 ~ 42 DEG C of fermentations 25 ~ 35 days or 50 DEG C ~ 58 DEG C ferment 12 ~ 16 days, obtain biogas residue and biogas liquid mixture;
(3) the biogas residue and biogas liquid mixture obtained in step (2) is passed through solid-liquid separation, obtain energy grass biogas residue and energy marsh Liquid;
(4) the energy grass biogas residue that step (3) separates is dried to moisture content 60 ~ 85wt%, is piled into 1 ~ 3 meter of high stacking, middle Every 30 ~ 50 centimetres of jacks, pore size 3 ~ 8 centimetres, cover with plastic foil, open plastic sheeting every day 1 ~ 3 time, each 10 ~ 60 minutes, pile up 15 ~ 30 days time;
(5) the energy grass biogas slurry that step (3) separates is carried out aeration, by weight, by Sal 5 ~ 10 parts, glucose or sucrose 5 ~ 10 parts, 1 ~ 3 part of carbamide, potassium dihydrogen phosphate 0.2 ~ 0.8 part add in 1600 ~ 2000 parts of clear water, boil 30 ~ 60 minutes, after cooling Seal and preserve, add the biogas slurry 100 ~ 150 parts after aeration before using, regulate pH 6.8 ~ 8.5, obtain edible mushroom cultivation nutrition liquid;
(6) by weight, by step (4) through accumulation after energy grass biogas residue 35 ~ 55 parts, step (1) gained energy grass fine powder 40 ~ 65 parts, Testa Tritici or 0 ~ 16 part of Testa oryzae, Gypsum Fibrosum powder or 2.5 ~ 3 parts of mix homogeneously of pulverized limestone, the edible fungi prepared by step (5) Cultivating nutrient solution regulation water content 55 ~ 70wt%, is 6.0 ~ 9.2 with lime water regulation pH, obtains culture medium of edible fungus;
(7) step (6) gained culture medium of edible fungus is built heap fermentation, the sterilization of mushroom bed, make the bed sowing, earthing, routinely technology control Temperature processed, humidity and illumination, cultivate hypha of edible fungus and fruiting;
(8), after edible mushroom 4 ~ 7 days, when cap basically forms, plant to planting material surface sprinkling step (5) gained edible fungi Cultivation nutrient fluid, it is 0.2 ~ 0.4 kilogram that every square metre of culture medium of edible fungus sprays edible mushroom cultivation nutrition liquid consumption;
(9) fruit body of edible fungi growth, ripe, gather;
(10) after fruit body of edible fungi is gathered, every 4 ~ 8 days, next tide mushroom walks to culture medium of edible fungus surface sprinkling after growing again Suddenly the edible mushroom cultivation nutrition liquid that prepared by (5), every square metre of culture medium of edible fungus sprays edible mushroom cultivation nutrition liquid 0.5 ~ 0.8 public affairs Jin, by clear water regulation culture medium of edible fungus water content to 55 ~ 70wt%;
(11) the second tide mushroom can be obtained according to step (8) ~ (9), continue to repeat step (10), (8) and (9), the three to five tide can be obtained Mushroom.
A kind of method utilizing energy grass biogas residue ridge-up bed method culturing edible fungus the most according to claim 1, it is characterised in that: Described energy grass is hybrid pennisetum, thin stem hybridization wolf tail, purple grassiness, dwarf elephant grass, red grassiness, grassiness, Sugarcane grass, huge bacterium Grass, Pennisetum hydridum, Taiwan Herba Hedyotis cantonensis, Sorghum propinquum, sugar grass, Arundo donax, phragmites communis, Plantula Neyraudiae Reynaudianae, Radix sacchari arundinacei, those special grass, arabian cron, America wolf tails Grass, Gui Mu, Nan Mu, platform agriculture, Fujian are herded, imperatoria, Caulis Miscanthis floriduli, Miscanthus sacchariflorus (Maxim) Benth et Hook f, Dicranopteris amplachinget Chiu, Arundinella hirta (Thunb.) Tanaka, Plantula Neyraudiae Reynaudianae, silagecorn, Herba Cymbopogonis Citrari, spend mutually One or more in rice grass, Value of Spartina Anglica, switchgrass, S. spontaneum.
A kind of method utilizing energy grass biogas residue ridge-up bed method culturing edible fungus the most according to claim 1, it is characterised in that: Described edible fungi is the one in Agaricus bisporus, Volvariella volvacea (Bull.Ex Franch.) Singer., Coprinus comatus, Caulis Bambusae In Taeniam, Brazilian mushroom, Stropharia rugoso-annulata, Pleurotus ostreatus.
CN201610414528.2A 2016-06-15 2016-06-15 A kind of method utilizing energy grass biogas residue ridge-up bed method culturing edible fungus Pending CN106083248A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106755126A (en) * 2017-01-20 2017-05-31 福建农林大学 A kind of method that utilization energy grass biogas residue prepares biological carbon
CN106811487A (en) * 2017-01-20 2017-06-09 福建农林大学 A kind of method of comprehensive utilization of energy grass and high protein kitchen garbage

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CN103752588A (en) * 2014-01-28 2014-04-30 福建农林大学 Comprehensive energy grass recycling method
CN103999694A (en) * 2014-06-09 2014-08-27 刘善勇 Circular production method for edible mushrooms

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
CN103752588A (en) * 2014-01-28 2014-04-30 福建农林大学 Comprehensive energy grass recycling method
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106755126A (en) * 2017-01-20 2017-05-31 福建农林大学 A kind of method that utilization energy grass biogas residue prepares biological carbon
CN106811487A (en) * 2017-01-20 2017-06-09 福建农林大学 A kind of method of comprehensive utilization of energy grass and high protein kitchen garbage

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