CN106070014B - A kind of individuation evaluation method of the animal learning memory based on water maze - Google Patents
A kind of individuation evaluation method of the animal learning memory based on water maze Download PDFInfo
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- CN106070014B CN106070014B CN201610428498.0A CN201610428498A CN106070014B CN 106070014 B CN106070014 B CN 106070014B CN 201610428498 A CN201610428498 A CN 201610428498A CN 106070014 B CN106070014 B CN 106070014B
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- 241001465754 Metazoa Species 0.000 title claims abstract description 61
- 238000011156 evaluation Methods 0.000 title claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 20
- 238000011534 incubation Methods 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 17
- 238000002474 experimental method Methods 0.000 claims abstract description 10
- 238000012347 Morris Water Maze Methods 0.000 claims abstract description 9
- 230000019771 cognition Effects 0.000 claims abstract description 8
- 230000002159 abnormal effect Effects 0.000 claims abstract description 5
- 238000012360 testing method Methods 0.000 claims abstract description 4
- 241000700157 Rattus norvegicus Species 0.000 claims description 9
- 238000012549 training Methods 0.000 claims description 9
- 230000009182 swimming Effects 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 3
- 208000019901 Anxiety disease Diseases 0.000 claims description 2
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- 230000009194 climbing Effects 0.000 abstract description 2
- 238000007796 conventional method Methods 0.000 abstract description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 14
- 208000010877 cognitive disease Diseases 0.000 description 12
- 230000003920 cognitive function Effects 0.000 description 12
- 238000011160 research Methods 0.000 description 9
- 238000007619 statistical method Methods 0.000 description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 201000010099 disease Diseases 0.000 description 6
- STECJAGHUSJQJN-GAUPFVANSA-N Hyoscine Natural products C1([C@H](CO)C(=O)OC2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-GAUPFVANSA-N 0.000 description 5
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- 229960002646 scopolamine Drugs 0.000 description 5
- 208000028698 Cognitive impairment Diseases 0.000 description 4
- 238000009811 bilateral tubal ligation Methods 0.000 description 4
- 241000700159 Rattus Species 0.000 description 3
- 206010012289 Dementia Diseases 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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Abstract
The individuation evaluation method for the animal learning memory based on water maze that the invention discloses a kind of, using Morris water maze laboratory as tool, using intact animal and abnormal animal is recognized as object, more batches big/mouse are analyzed to time (incubation period) data for climbing up platform from entering water, determine the abnormal animal individual evaluation method of cognition.Compared with the method for traditional comparison among groups for emphasizing sample size, the present invention obviates the way taken a part for the whole of " mean+SD " form simple in conventional method, but animal pattern is separated by the otherness of cognition, studies one by one.This method can more objectively reflect the otherness of animal cognition level, closer to the time of day of animal.This method is easy to operate, feasible, and more batches of animals repeat to test the reliability and science for also ensuring method with the confirmatory experiment of model.
Description
Technical field
The individuation evaluation method for the animal learning memory based on water maze that the present invention relates to a kind of
Background technique
Morris water maze is the detection device for evaluating big/learning and memory of little mouse function generally acknowledged at present, it is to exclude gas
Taste interference, environmental disturbances, experimental period is short, is widely used without a variety of advantages such as fasting.But there is also numbers simultaneously for it
According to the disadvantages of lengthy and jumbled, processing is cumbersome.Traditional statistical method is mostly used to the data processing method of Morris water maze at present,
I.e. under the premise of defaulting the generation cognitive impairment of blank control group 0%, compared with the control group by model group, compare the significant of the two
Sex differernce.It is 100% that this way, which is equal to default cognitive impairment disease incidence, although meeting mathematical statistics rule, is not inconsistent
Close actual conditions.Clinically the disease incidence of the cognitive disorder as caused by diabetes is usually 30%-50%, even blank control
Group is also likely to be present the cognitive disorder within 10%.Simultaneously because the disease incidence of traditional evaluation method default animal cognition obstacle
It is 100%, is lack of pertinence when if allowing for using drug interventional therapy, similarly includes a part when evaluating drug effect
Normal animal itself.Therefore traditional data analysing method there is scientific insufficient and can not comment animal progress individual
The disadvantages of valence.In view of the above problems, establishing the evaluation criterion of a cognitive impairment, the animal that individuation analyzes cognitive impairment is ten
Divide necessity.
Summary of the invention
The present invention is directed to determine that the abnormal animal individual evaluation method of cognition, this method are a kind of dynamic based on water maze
The individuation evaluation method of object learning and memory, this method are as follows:
Learning and memory experiment to animal is carried out using Morris water maze, with animal from water is entered to the time for climbing up platform
As the index of evaluation school grade, as incubation period carries out data processing to incubation period, from training the 6th time to the 10th coming half year
Between, if there are continuous 2 incubation periods and is more than or equal to 90s in animal, and without there are continuous 2 incubation periods lower than Normal group the
The case where 3 trained average latencies, then it is assumed that it is abnormal that cognition occurs in animal；
Learning and memory detecting step of its Morris water maze to animal are as follows:
Step 1: on the day before formal experiment, all animals free swimming in the water maze for not being laid flat platform and marker
2min avoids animal stress reaction to adapt to environment in water；
Step 2: formal experiment starts, it is trained phase test first, when training period, puts animal before experiment flat
Station 10s helps animal to establish impression；
Step 3: in different quadrants by animal into the water, animal swimming time is 120s, incubation period is recorded, if dynamic
Object does not find platform in 120s, then incubation period is denoted as 120s, and platform then is climbed up in animal guidance allows it to stop on platform
10s is stayed, to reduce animal anxiety；
Step 4: 1-4 hours time of every animal interval repeats above-mentioned experimentation, and guarantee every batch of animal interval
Time consistency.
Preferably, the animal is healthy adult Wistar rat or healthy adult ICR mouse.
Beneficial effect
The present invention obviates in conventional method the way taken a part for the whole of simple " mean+SD " form, can be
Cognitive function exception and normal animal are distinguished in each Group Animals.The present invention can more objectively reflect animal cognition level
Otherness, closer to animal time of day.This method is easy to operate, feasible, and more batches of animals repeat to test and be respectively adopted
Hyoscine and bilateral ligation establish dull-witted and cognitive disorder model confirmatory experiment and also ensure the reliable of method
Property and science.
Specific embodiment
Below with reference to embodiment, the present invention is described in more detail, but the present invention is not limited to these Examples.
Embodiment 1
The individuation evaluation method of the animal learning memory based on water maze of this implementation is to recognize normal ICR individual mice
Know functional study, research process is as follows:
20 weight 20 ± 2g, 4-5 week old, half male and half female are chosen, ICR mouse carries out the research of Morris water maze laboratory.
According to traditional statistical method, then this group is blank control group, then this group cognitive disorder mouse occurs and is defaulted as 0%.But it adopts
It is found with this study on evaluation way, it is 94.8s that trained 3rd time, which is ICR mouse incubation period, during 6th time to the 10th time trained,
Cognitive function exception mouse is 2, and cognitive function disease incidence is 10.0% (the results are shown in Table 1), compared with traditional statistical method, this
One data more meet practical rule, it is believed that evaluation method success.
The normal ICR mouse of table 1 trains incubation period result (s) every time
Embodiment 2
The individuation evaluation method of the animal learning memory based on water maze of this implementation is of normal Wistar rats
Body Cognitive Function Research, research process are as follows:
20 weight 200 ± 20g, 6-8 week old, half male and half female are chosen, Wistar rat carries out Morris water maze laboratory
Research.According to traditional statistical method, this group is blank control group, then this group cognitive disorder rat occurs and is defaulted as 0%.But
It is found using this study on evaluation way, it is 58.0s that training the 3rd time, which is Wistar rat incubation period, from training the 6th time to the 10th time
Period, cognitive function exception mouse are 1, and cognitive function disease incidence is 5.0% (the results are shown in Table 2), with traditional statistical method phase
Than this data more meets practical rule, it is believed that evaluation method success.
2 normal Wistar rats of table train incubation period result (s) every time
Embodiment 3
The individuation evaluation method of the animal learning memory based on water maze of this implementation is to inject the mouse of hyoscine
Cognitive function research, research process are as follows:
Cognitive disorder model is established using animal intraperitoneal injection hyoscine.20 weight 20 ± 2g, 4-5 week old are chosen,
Half male and half female, hyoscine solution 1mg/kg is injected intraperitoneally in 20min before water maze laboratory, establishes mouse Model of Dementia.According to
Traditional statistical method, this group are model control group, then this group cognitive disorder mouse occurs and is defaulted as 100%.But it uses and originally comments
The discovery of valence technique study, from during training the 6th time to the 10th time, cognitive function exception mouse is 16, cognitive function disease incidence
For 80.0% (the results are shown in Table 3), compared with traditional statistical method, this data more meets practical rule, it is believed that evaluation method
Success.
3 hyoscine of table trains preclinical influence (s) to ICR mouse every time
Embodiment 4
The individuation evaluation method of the animal learning memory based on water maze of this implementation is bilateral ligation
Rat cognitive function research, research process are as follows:
Choose 20 weight 200 ± 20g, 6-8 week old, half male and half female Wistar rat, using bilateral ligation
Vascular cognitive impairment model is established, the evaluation of learning and memory can be carried out after model foundation 7 days.The present embodiment selects model to build
42nd day rat after vertical, the 3rd Wistar rat incubation period of training is 55.3s, and according to traditional statistical method, this group is mould
Type control group, then there is cognitive disorder mouse and is defaulted as 100% in this group.But this study on evaluation way is used to find, from training
During 6th time to the 10th time, cognitive function exception mouse is 11, and cognitive function disease incidence is 55.0% (the results are shown in Table 4), with
Traditional statistical method is compared, this data more meets practical rule, it is believed that evaluation method success.
4 bilateral ligation of table trains preclinical influence (s) to Wistar rat every time
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (2)
1. a kind of individuation evaluation method of the animal learning memory based on water maze, it is characterised in that this method is as follows:
Carried out using Morris water maze to the experiment of the learning and memory of animal, using animal from enter water to climb up time of platform as
The index of school grade, as incubation period are evaluated, data processing is carried out to incubation period, from the 6th time to the 10th time period of training, if
There are continuous 2 incubation periods more than or equal to 90s in animal, and without there is lower than Normal group the 3rd time instruction of continuous 2 incubation periods
The case where experienced average latency, then it is assumed that it is abnormal that cognition occurs in animal；
Learning and memory detecting step of its Morris water maze to animal are as follows:
Step 1: on the day before formal experiment, all animals free swimming 2min in the water maze for not being laid flat platform and marker,
To adapt to environment in water, animal stress reaction is avoided；
Step 2: formal experiment starts, it is trained phase test first, when training period, animal is put into platform station before experiment
10s helps animal to establish impression；
Step 3: in different quadrants by animal into the water, animal swimming time be 120s, record incubation period, if animal exists
Platform is not found in 120s, then incubation period is denoted as 120s, and platform then is climbed up in animal guidance allows it to stop on platform
10s, to reduce animal anxiety；
Step 4: 1-4 hours time of every animal interval repeats above-mentioned experimentation, and guarantee every batch of animal interval time
Unanimously.
2. the individuation evaluation method of animal learning memory according to claim 1, it is characterised in that: the animal is strong
Health adult Wistar rats or healthy adult ICR mouse.
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| CN107318691A (en) * | 2017-07-18 | 2017-11-07 | 中国科学技术大学 | A kind of training method of trapezoidal water maze visual discrimination task and reversion learning tasks |
| CN110101718A (en) * | 2019-05-14 | 2019-08-09 | 浙江理工大学 | A kind of research method of production and function based on silkworm chrysalis mix preparation |
| CN113940286A (en) * | 2021-09-30 | 2022-01-18 | 南通大学 | Method for training and improving space learning capacity of mouse |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499105A (en) * | 2011-10-06 | 2012-06-20 | 中国医学科学院医学生物学研究所 | Method for building a water maze spatial learning and memory model for tree shrews |
| CN203912910U (en) * | 2014-07-01 | 2014-11-05 | 苏州大学 | A kind of water maze |
| CN205124681U (en) * | 2015-10-30 | 2016-04-06 | 江苏大学 | Mouse water maze study memory test platform |
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| US20040229292A1 (en) * | 2002-11-26 | 2004-11-18 | Sebastiano Cavallaro | Use of FGF-18 in the diagnosis and treatment of memory disorders |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499105A (en) * | 2011-10-06 | 2012-06-20 | 中国医学科学院医学生物学研究所 | Method for building a water maze spatial learning and memory model for tree shrews |
| CN203912910U (en) * | 2014-07-01 | 2014-11-05 | 苏州大学 | A kind of water maze |
| CN205124681U (en) * | 2015-10-30 | 2016-04-06 | 江苏大学 | Mouse water maze study memory test platform |
Non-Patent Citations (1)
| Title |
|---|
| 基于Morris水迷宫学习记忆方法的研究;余群星;《中国优秀硕士学位论文全文数据库 医药卫生科技辑》;20150831(第8期);E071-25 |
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