CN106069756B - A kind of quick breeding method for tissue culture of white flower Ornithogalum caudatum - Google Patents
A kind of quick breeding method for tissue culture of white flower Ornithogalum caudatum Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
This application discloses a kind of quick breeding method for tissue culture of white flower Ornithogalum caudatum, chooses explant of the blade of white flower Ornithogalum caudatum as tissue culture；Seedling is formed by blade surface bulbil Fiber differentiation, or seedling is formed by Callus of Leaf Fiber differentiation, adventitious bud induction culture, culture of rootage；After seedling grows adventitious root, tissue-cultured seedling rooting culture is carried out, then normal culture.The quick breeding method for tissue culture of the application white flower Ornithogalum caudatum; solve the problems, such as low in the presence of regeneration difficulty, bulb reproduction coefficient using underground propagation by corm; with the high remarkable advantage of easy to operate, growth coefficient; do not limited by area, season, weather, the industrial massive production of high quality seedling can be achieved.
Description
Technical field
The application belongs to technical field of plant propagation, specifically, is related to a kind of tissue cultures of white flower Ornithogalum caudatum
Rapid propagation method.
Background technology
White flower Ornithogalum caudatum (Ornithogalum thyroides) is Liliaceae Ornithogalum
The perennial bulb flowers of (Ornithogalum spp), high 25~50 centimetres, tunicated bulb circle is smooth, and ornamental value is high.
Leaf base is grown thickly, lanceolar and grow, top tool long-tail, bottle green, stockless.Spend small-sized, raceme is close, pyramid, raw
In at the top of the scape of pumping, above little Hua more than 25, flower-shape cup-shaped, pattern is white, petal center redgreen striped from leafage.
Not only its flower and bulb have higher ornamental value, can be widely applied to afforestation and as Fresh Cutting flower,
And chemical composition analysis shows, wherein containing secondary metabolites such as steroline, cholestane glucosides, have potential medicinal
And economic value.
With the development of economic society and gardens cause, flowering bulb because with species is abundant, pattern is gorgeous, the florescence compared with
Long, cultivation easily, strong adaptability the features such as and advantage, using more and more in gardens, can be widely used as cut-flower, quilt and basin
Flower etc., also has higher medicinal and economic value.Ornithogalum caudatum's platymiscium has as the perennial flower bulb of Liliaceae
Kind more than 150, because it has higher ornamental value, and higher medical value, more and more attracted attention.
But no matter out of office survive of breeding of flowering bulb is in vitro, substantially using the bulb of underground, generally
There is regeneration is difficult, bulb propagation coefficient is low and the outstanding problems such as germplasm innovation is difficult, Ornithogalum caudatum's platymiscium is no exception,
As the bottleneck of its research and development of restriction.
The content of the invention
In view of this, the application is directed to has the problem of regeneration is difficult, bulb reproduction coefficient is low using underground propagation by corm,
A kind of quick breeding method for tissue culture of white flower Ornithogalum caudatum is provided, there is high notable of easy to operate, growth coefficient
Advantage, the industrial massive production of high quality seedling can be achieved.
In order to solve the above-mentioned technical problem, this application discloses a kind of tissue-culturing quick-propagation of white flower Ornithogalum caudatum
Method, choose explant of the blade of white flower Ornithogalum caudatum as tissue culture；Formed by blade surface bulbil Fiber differentiation
Seedling, or seedling is formed by Callus of Leaf Fiber differentiation, adventitious bud induction culture, culture of rootage；Treat that seedling is grown
After adventitious root, tissue-cultured seedling rooting culture is carried out, then normal culture.
Further, following steps are specifically included：
Step 1, the selection and processing of explant
The blade for choosing white flower Ornithogalum caudatum is parent material, and the blade collected is cut into 2~4cm leaf section, is made
For the explant of tissue culture；
Step 2, induced synthesis seedling is carried out to blade
Explant Jing Guo surface sterilization is inoculated on bulbil inducing culture MS+TDZ2.0mg/L and trained at illumination
Support, after blade surface gradually forms complete root system, root system, which is inserted in culture medium, turns into new seedling；
Or that the explant Jing Guo surface sterilization is inoculated in into callus inducing medium MS+2,4D2.0mg/L is enterprising
Row light culture, treat to form white or flaxen callus at paddle cutout, be then inoculated in adventitious bud induction culture base MS+
6BA2.0mg/L, it is placed at illumination and cultivates, callus gradually forms adventitious bud, then is transferred to root media MS+
On IBA1.0mg/L, after 20~30d, seedling of taking root turns into new seedling；
Step 3, tissue-cultured seedling rooting culture
After the adventitious root that seedling grows 3~5 2~3cm, hardening is carried out, tissue-cultured seedling is then transplanted to culture matrix
Middle carry out slow seedling, then normal culture.
Further, cultivation temperature is 25 DEG C.
Further, light application time is 12~14h/d, and intensity of illumination is 40~60u.Mol.m-2.s-1。
Further, bulbil induction, callus induction, adventitious bud inducing and culture of rootage, minimal medium used
It is MS culture mediums, dosage of sucrose is 20~40g/L, and coagulator is agar powder, dosage is 6~7g/L, medium pH 5.8.
Further, the hardening is specially：First in tissue culture chamber opening bottle cap hardening 3 days, be then transported to greenhouse or
Cool canopy, finishing scouring seedling 3 days.
Further, the culture matrix is by sandy loam and turfy soil in mass ratio 3:1 composition.
Further, within the water content 25% of the culture matrix, relative air humidity 80~90%.
Compared with prior art, the application can be obtained including following technique effect：
(1) the application is used as the explant of tissue culture by choosing the blade of white flower Ornithogalum caudatum；Pass through blade surface
Bulbil Fiber differentiation forms seedling, or is formed by Callus of Leaf Fiber differentiation, adventitious bud induction culture, culture of rootage
Seedling；After seedling grows adventitious root, tissue-cultured seedling rooting culture is carried out, then normal culture, is solved numerous using underground bulb
Grow in the presence of the problem of regeneration is difficult, bulb reproduction coefficient is low, there is remarkable advantage easy to operate, growth coefficient is high, not by ground
Area, season, weather limitation, the industrial massive production of high quality seedling can be achieved.
(2) blade is cut from maternal plant, using blade as explant, forms multiple bulbils on a blade, breeding coefficient is up to 3
~10, it is more than ten times of general modes of reproduction even tens times, and plant is damaged in itself less, maternal plant can be with continued growth
And utilization, realize sustainable production and development.
(3) high-efficiency regeneration system of white flower Ornithogalum caudatum is established, is the industrialization development of white flower Ornithogalum caudatum, establishes
The good technical support of the offer such as molecular breeding platform and Germplasm enhancement and system support；To improve white flower Ornithogalum caudatum
Genetic conversion system, establish the platform of molecular breeding, genetic improvement, further investigation utilization carried out to it from molecular level
Lay a good foundation.
(4) the application can also provide beneficial thinking and reference for the plant of other flowering bulbs and regeneration difficulty.
Certainly, any product for implementing the application it is not absolutely required to reach all the above technique effect simultaneously.
Brief description of the drawings
Accompanying drawing described herein is used for providing further understanding of the present application, forms the part of the application, this Shen
Schematic description and description please is used to explain the application, does not form the improper restriction to the application.In the accompanying drawings：
Fig. 1 is the tissue-culturing quick-propagation process comparison diagram of the embodiment of the present application white flower Ornithogalum caudatum；In figure, A is
White flower Ornithogalum caudatum's blade, B are that white flower Ornithogalum caudatum's blade surface forms multiple bulbils, C be blade surface bulbil by
Gradually sprout, D is that bulbil is taken root seedling, and E is that blade produces callus and callus differentiates adventitious bud, and F is white flower tiger eye
Evergreen kind of seedling rooting, G are that white flower Ornithogalum caudatum forms bud, and H is that white flower Ornithogalum caudatum is bloomed.
Embodiment
Describe presently filed embodiment in detail below in conjunction with embodiment, thereby to the application how application technology hand
Section can fully understand and implement according to this to solve technical problem and reach the implementation process of technical effect.
The quick breeding method for tissue culture of white flower Ornithogalum caudatum of the present invention, specifically includes following steps：
Step 1, the selection and processing of explant
The blade for choosing white flower Ornithogalum caudatum is parent material, and the blade collected is cut into 2~4cm leaf section, is made
For the explant of tissue culture；
Step 2, induced synthesis seedling is carried out to blade
Step 2-1, the leaf section Jing Guo surface sterilization is transferred in aseptic inoculation disk, and leaf section surface is blotted with aseptic filter paper
Moisture；
Specifically, described sterilize is specially：White flower Ornithogalum caudatum's leaf section is cleaned into 30min with liquid detergent solution, during which
Constantly it is gently mixed, then flowing water rinses 30~60min, by the above-mentioned white flower cleaned on sterilized superclean bench
Ornithogalum caudatum's leaf section is transferred in sterile beaker, with alcohol-pickled 30~60s that volumetric concentration is 75%, is during which constantly rocked
Beaker, alcohol is poured out, then 3~5min is soaked with the mercuric chloride of mass concentration 0.1%, constantly gently rocked during immersion, pour out liter
After mercury, with blade more than 3 time of the sterile water wash by sterilization.
Step 2-2, the leaf section after sterilization is kept flat or inserted bulbil inducing culture MS+TDZ2.0mg/L (TDZ：Thiophene benzene
It is grand) on carry out bulbil induction, as shown in Fig. 1 (A), 25 DEG C of cultivation temperature, light application time is 12~14h/d, and intensity of illumination is
40~60u.Mol.m-2.s-1；20d rear blades surface forms small particles, as shown in Fig. 1 (B), the gradual greening of small particles, after
After 20d, small particles becomes green ball, and as shown in Fig. 1 (C), green ball is slowly taken root, and eventually forms complete root system, such as Fig. 1 (D) institute
Show, root system, which is inserted in culture medium, turns into new seedling, i.e., " is taken root on leaf, land seedling "；
Or the leaf section after sterilization is inoculated in callus inducing medium MS+2,4D2.0mg/L (2,4D：2,4- bis-
Chlorophenoxyacetic acid) on be placed at dark carry out callus induction, 25 DEG C of temperature, white is formed after 20d at paddle cutout
Or flaxen callus；Then the callus induced is transferred to adventitious bud induction culture base MS+6BA2.0mg/L
(6BA：Benayl aminopurine) carry out adventitious bud induction, 25 DEG C of cultivation temperature, light application time is 12~14h/d, and intensity of illumination is
40~60u.Mol.m-2.s-1, callus gradually forms adventitious bud (blade) after 20d, as shown in Fig. 1 (E)；It will induce again
Adventitious bud be transferred to root media MS+IBA1.0mg/L (IBA：Indolebutyric acid) on carry out adventitious root induction, 20~30d
Afterwards, seedling of taking root turns into new seedling, as shown in Fig. 1 (F)；
Wherein, bulbil induction, callus induction, adventitious bud inducing and culture of rootage, minimal medium used are
MS culture mediums, dosage of sucrose are 20~40g/L, and coagulator is agar powder, dosage is 6~7g/L, medium pH 5.8.
Step 3, tissue-cultured seedling rooting culture
After the adventitious root that seedling grows 3~5 2~3cm, hardening is carried out, then cleans the agar on tissue-cultured seedling surface,
It is transplanted in culture matrix and carries out slow seedling, as shown in Fig. 1 (G), tissue-cultured seedling is transplanted in basin and forms bud, then normal culture,
As shown in Fig. 1 (H), the white flower Ornithogalum caudatum indefinite inflorescence of breeding opens one month successively from bottom to up.
The hardening is specially：First in tissue culture chamber opening bottle cap hardening 3d, greenhouse or cool canopy, then hardening are then transported to
3d。
Preferably, culture matrix is by sandy loam and turfy soil in mass ratio 3:1 composition, the water content 25% of culture matrix with
It is interior, relative air humidity 80~90%.
Embodiment 1
Step 1, the selection and processing of explant
The blade for choosing white flower Ornithogalum caudatum is parent material, the blade collected is cut into 3cm leaf section, as group
The explant of training；
Step 2, induced synthesis seedling is carried out to blade
Step 2-1, the leaf section Jing Guo surface sterilization is transferred in aseptic inoculation disk, and leaf section surface is blotted with aseptic filter paper
Moisture；
Step 2-2, the leaf section after sterilization is kept flat or inserted on bulbil inducing culture MS+TDZ2.0mg/L and carries out bulbil
Induction, 25 DEG C, light application time 14h/d, intensity of illumination 40u.Mol.m of cultivation temperature-2.s-1；Treat the gradual shape of blade surface
Into after complete root system, root system, which is inserted in culture medium, turns into new seedling；
Minimal medium used is MS culture mediums, and dosage of sucrose 30g/L, coagulator is agar powder, dosage 6.5g/
L, medium pH 5.8.
Step 3, tissue-cultured seedling rooting culture
After the adventitious root that seedling grows 3 3cm, hardening is carried out, then the agar on tissue-cultured seedling surface is cleaned, is transplanted to
Slow seedling is carried out in culture matrix, culture matrix is by sandy loam and turfy soil in mass ratio 3:1 composition, the water content of culture matrix
Within 25%, relative air humidity 80%, then normal culture.
Embodiment 2
Step 1, the selection and processing of explant
The blade for choosing white flower Ornithogalum caudatum is parent material, the blade collected is cut into 2cm leaf section, as group
The explant of training；
Step 2, induced synthesis seedling is carried out to blade
Step 2-1, the leaf section Jing Guo surface sterilization is transferred in aseptic inoculation disk, and leaf section surface is blotted with aseptic filter paper
Moisture；
Step 2-2, the leaf section after sterilization is kept flat or inserted on bulbil inducing culture MS+TDZ2.0mg/L and carries out bulbil
Induction, 25 DEG C, light application time 13h/d, intensity of illumination 50u.Mol.m of cultivation temperature-2.s-1；Treat the gradual shape of blade surface
Into after complete root system, root system, which is inserted in culture medium, turns into new seedling；
Minimal medium used is MS culture mediums, dosage of sucrose 20g/L, and coagulator is agar powder, dosage 6g/L,
Medium pH is 5.8.
Step 3, tissue-cultured seedling rooting culture
After the adventitious root that seedling grows 4 3cm, hardening is carried out, then the agar on tissue-cultured seedling surface is cleaned, is transplanted to
Slow seedling is carried out in culture matrix, culture matrix is by sandy loam and turfy soil in mass ratio 3:1 composition, the water content of culture matrix
Within 25%, relative air humidity 85%, then normal culture.
Embodiment 3
Step 1, the selection and processing of explant
The blade for choosing white flower Ornithogalum caudatum is parent material, the blade collected is cut into 4cm leaf section, as group
The explant of training；
Step 2, induced synthesis seedling is carried out to blade
Step 2-1, the leaf section Jing Guo surface sterilization is transferred in aseptic inoculation disk, and leaf section surface is blotted with aseptic filter paper
Moisture；
Step 2-2, the leaf section after sterilization is kept flat or inserted on bulbil inducing culture MS+TDZ2.0mg/L and carries out bulbil
Induction, 25 DEG C, light application time 12h/d, intensity of illumination 60u.Mol.m of cultivation temperature-2.s-1；Treat the gradual shape of blade surface
Into after complete root system, root system, which is inserted in culture medium, turns into new seedling；
Minimal medium used is MS culture mediums, dosage of sucrose 40g/L, and coagulator is agar powder, dosage 7g/L,
Medium pH is 5.8.
Step 3, tissue-cultured seedling rooting culture
After the adventitious root that seedling grows 5 2cm, hardening is carried out, then the agar on tissue-cultured seedling surface is cleaned, is transplanted to
Slow seedling is carried out in culture matrix, culture matrix is by sandy loam and turfy soil in mass ratio 3:1 composition, the water content of culture matrix
Within 25%, relative air humidity 90%, then normal culture.
Embodiment 4
Step 1, the selection and processing of explant
The blade for choosing white flower Ornithogalum caudatum is parent material, the blade collected is cut into 3cm leaf section, as group
The explant of training；
Step 2, induced synthesis seedling is carried out to blade
Step 2-1, the leaf section Jing Guo surface sterilization is transferred in aseptic inoculation disk, and leaf section surface is blotted with aseptic filter paper
Moisture；
Step 2-2, the leaf section after sterilization is inoculated on callus inducing medium MS+2,4D2.0mg/L be placed in it is black
Dark place carries out the induction of callus, 25 DEG C of temperature；Then the callus induced is transferred to adventitious bud induction culture base
MS+6BA2.0mg/L carries out the induction of adventitious bud, 25 DEG C, light application time 14h/d of cultivation temperature, and intensity of illumination is
40u.Mol.m-2.s-1；The adventitious bud induced is transferred on root media MS+IBA1.0mg/L again and carries out adventitious root
Induce, after 20~30d, seedling of taking root turns into new seedling；
Minimal medium used is MS culture mediums, and dosage of sucrose 30g/L, coagulator is agar powder, dosage is
6.5g/L, medium pH 5.8.
Step 3, tissue-cultured seedling rooting culture
After the adventitious root that seedling grows 3 3cm, hardening is carried out, then the agar on tissue-cultured seedling surface is cleaned, is transplanted to
Slow seedling is carried out in culture matrix, culture matrix is by sandy loam and turfy soil in mass ratio 3:1 composition, the water content of culture matrix
Within 25%, relative air humidity 80%, then normal culture.
Embodiment 5
Step 1, the selection and processing of explant
The blade for choosing white flower Ornithogalum caudatum is parent material, the blade collected is cut into 2cm leaf section, as group
The explant of training；
Step 2, induced synthesis seedling is carried out to blade
Step 2-1, the leaf section Jing Guo surface sterilization is transferred in aseptic inoculation disk, and leaf section surface is blotted with aseptic filter paper
Moisture；
Step 2-2, the leaf section after sterilization is inoculated on callus inducing medium MS+2,4D2.0mg/L be placed in it is black
Dark place carries out the induction of callus, 25 DEG C of temperature；Then the callus induced is transferred to adventitious bud induction culture base
MS+6BA2.0mg/L carries out the induction of adventitious bud, 25 DEG C, light application time 13h/d of cultivation temperature, and intensity of illumination is
50u.Mol.m-2.s-1；The adventitious bud induced is transferred on root media MS+IBA1.0mg/L again and carries out adventitious root
Induce, after 20~30d, seedling of taking root turns into new seedling；
Minimal medium used is MS culture mediums, and dosage of sucrose 20g/L, coagulator is agar powder, dosage 6g/
L, medium pH 5.8.
Step 3, tissue-cultured seedling rooting culture
After the adventitious root that seedling grows 4 3cm, hardening is carried out, then the agar on tissue-cultured seedling surface is cleaned, is transplanted to
Slow seedling is carried out in culture matrix, culture matrix is by sandy loam and turfy soil in mass ratio 3:1 composition, the water content of culture matrix
Within 25%, relative air humidity 85%, then normal culture.
Embodiment 6
Step 1, the selection and processing of explant
The blade for choosing white flower Ornithogalum caudatum is parent material, the blade collected is cut into 4cm leaf section, as group
The explant of training；
Step 2, induced synthesis seedling is carried out to blade
Step 2-1, the leaf section Jing Guo surface sterilization is transferred in aseptic inoculation disk, and leaf section surface is blotted with aseptic filter paper
Moisture；
Step 2-2, the leaf section after sterilization is inoculated on callus inducing medium MS+2,4D2.0mg/L be placed in it is black
Dark place carries out the induction of callus, 25 DEG C of temperature；Then the callus induced is transferred to adventitious bud induction culture base
MS+6BA2.0mg/L carries out the induction of adventitious bud, 25 DEG C, light application time 12h/d of cultivation temperature, and intensity of illumination is
60u.Mol.m-2.s-1；The adventitious bud induced is transferred on root media MS+IBA1.0mg/L again and carries out adventitious root
Induce, after 20~30d, seedling of taking root turns into new seedling；
Minimal medium used is MS culture mediums, and dosage of sucrose 40g/L, coagulator is agar powder, dosage 7g/
L, medium pH 5.8.
Step 3, tissue-cultured seedling rooting culture
After the adventitious root that seedling grows 5 2cm, hardening is carried out, then the agar on tissue-cultured seedling surface is cleaned, is transplanted to
Slow seedling is carried out in culture matrix, culture matrix is by sandy loam and turfy soil in mass ratio 3:1 composition, the water content of culture matrix
Within 25%, relative air humidity 90%, then normal culture.
The culture of white flower Ornithogalum caudatum's leaf tissue is bred using the above method, breeding coefficient is general numerous up to 3~10
More than ten times of mode even tens times are grown, and plant is damaged in itself less, maternal plant can with continued growth and utilization, realization
Continuous production and development, and do not limited by area, season, weather, the industrial massive production of high quality seedling can be achieved.
Some vocabulary has such as been used to censure special component or method among specification and claim.Art technology
Personnel are, it is to be appreciated that different regions may call same composition with different nouns.This specification and claims are not
In a manner of the difference of title is used as and distinguishes composition.As the "comprising" of the specification in the whole text and claim mentioned in is
One open language, therefore " include but be not limited to " should be construed to." substantially " refer in receivable error range, this area
Technical staff can solve the technical problem within a certain error range, basically reach the technique effect.Specification is follow-up
It is described as implementing the better embodiment of the application, so description is for the purpose of the rule for illustrating the application, not
To limit scope of the present application.The protection domain of the application is worked as to be defined depending on appended claims institute defender.
It should also be noted that, term " comprising ", "comprising" or its any other variant are intended to nonexcludability
Comprising, so that commodity or system including a series of elements not only include those key elements, but also including without clear and definite
The other element listed, or also include for this commodity or the intrinsic key element of system.In the feelings not limited more
Under condition, the key element that is limited by sentence "including a ...", it is not excluded that in the commodity including the key element or system also
Other identical element be present.
Some preferred embodiments of invention have shown and described in described above, but as previously described, it should be understood that invention is not
Form disclosed herein is confined to, is not to be taken as the exclusion to other embodiment, and available for various other combinations, modification
And environment, and can be carried out in the scope of the invention is set forth herein by the technology or knowledge of above-mentioned teaching or association area
Change., then all should be in power appended by invention and the change and change that those skilled in the art are carried out do not depart from the spirit and scope of invention
In the protection domain that profit requires.
Claims (7)
1. a kind of quick breeding method for tissue culture of white flower Ornithogalum caudatum, it is characterised in that choose white flower Ornithogalum caudatum
Explant of the blade as tissue culture；Seedling is formed by blade surface bulbil Fiber differentiation, or passes through blade callus group
Knit Fiber differentiation, adventitious bud induction culture, culture of rootage and form seedling；After seedling grows adventitious root, tissue-cultured seedling domestication is carried out
Transplanting, then normal culture；Specifically include following steps：
Step 1, the selection and processing of explant
The blade for choosing white flower Ornithogalum caudatum is parent material, the blade collected is cut into 2~4cm leaf section, as group
The explant of training；
Step 2, induced synthesis seedling is carried out to blade
Explant Jing Guo surface sterilization is inoculated on bulbil inducing culture MS+TDZ2.0mg/L and cultivated at illumination, is treated
After blade surface gradually forms complete root system, root system, which is inserted in culture medium, turns into new seedling；
Or the explant Jing Guo surface sterilization is inoculated on callus inducing medium MS+2,4D2.0mg/L and carried out secretly
Culture, treats to form white or flaxen callus at paddle cutout, is then inoculated in adventitious bud induction culture base MS+
6BA2.0mg/L, it is placed at illumination and cultivates, callus gradually forms adventitious bud, then is transferred to root media MS+
On IBA1.0mg/L, after 20~30d, seedling of taking root turns into new seedling；
Step 3, tissue-cultured seedling rooting culture
After the adventitious root that seedling grows 3~5 2~3cm, hardening is carried out, then is transplanted in culture matrix by tissue-cultured seedling
Row slow seedling, then normal culture.
2. the quick breeding method for tissue culture of the white flower Ornithogalum caudatum as described in profit requires 1, it is characterised in that cultivation temperature
For 25 DEG C.
3. the quick breeding method for tissue culture of the white flower Ornithogalum caudatum as described in profit requires 1, it is characterised in that light application time
For 12~14h/d, intensity of illumination is 40~60u.Mol.m-2.s-1。
4. the quick breeding method for tissue culture of the white flower Ornithogalum caudatum as described in profit requires 1, it is characterised in that bulbil lures
Lead, callus induction, adventitious bud inducing and culture of rootage, minimal medium used are MS culture mediums, dosage of sucrose is
20~40g/L, coagulator is agar powder, dosage is 6~7g/L, medium pH 5.8.
5. the quick breeding method for tissue culture of the white flower Ornithogalum caudatum as described in profit requires 1, it is characterised in that the hardening
Specially：First in tissue culture chamber opening bottle cap hardening 3 days, greenhouse or cool canopy, finishing scouring seedling 3 days are then transported to.
6. the quick breeding method for tissue culture of the white flower Ornithogalum caudatum as described in profit requires 1, it is characterised in that the culture
Matrix is by sandy loam and turfy soil in mass ratio 3:1 composition.
7. the quick breeding method for tissue culture of the white flower Ornithogalum caudatum as described in profit requires 1 or 6, it is characterised in that described
Within the water content 25% of culture matrix, relative air humidity 80~90%.
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| CN106508684B (en) * | 2016-12-08 | 2018-08-03 | 云南省热带作物科学研究所 | A kind of South Africa Star of Bethlehem in-vitro culture method |
| CN109430059A (en) * | 2018-12-25 | 2019-03-08 | 云南省农业科学院花卉研究所 | A kind of cut-flower Ornithogalum caudatum in vitro culture quick-breeding method |
| CN111118027B (en) * | 2020-01-17 | 2022-07-29 | 四川天艺优境环境科技有限公司 | Ornithogalum caudatum ait homologous structure domain transcription factor OtPHD1 gene and application |
| CN112243631B (en) * | 2020-09-14 | 2022-03-22 | 云南省农业科学院花卉研究所 | Method for rapidly breaking dormancy of green flower lily seed bulbs |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103858773A (en) * | 2014-04-11 | 2014-06-18 | 北京农学院 | Method for rapid propagation in vitro of ornithogalum saundersiae by taking flower stalk as explant |
| CN103931498A (en) * | 2014-04-11 | 2014-07-23 | 北京农学院 | In-vitro rapid propagation method for ornithogalum caudatum by taking infant flowers as explants |
-
2016
- 2016-06-16 CN CN201610431551.2A patent/CN106069756B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103858773A (en) * | 2014-04-11 | 2014-06-18 | 北京农学院 | Method for rapid propagation in vitro of ornithogalum saundersiae by taking flower stalk as explant |
| CN103931498A (en) * | 2014-04-11 | 2014-07-23 | 北京农学院 | In-vitro rapid propagation method for ornithogalum caudatum by taking infant flowers as explants |
Non-Patent Citations (1)
| Title |
|---|
| 虎眼万年青离体快繁体系及无糖生根培养;屈云慧等;《中南林学院学》;20031031;第23卷(第5期);第57页第1.2-1.3节，第2.1节，第58页第2段 * |
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