CN106066306A - A kind of photoacoustic microscope system - Google Patents
A kind of photoacoustic microscope system Download PDFInfo
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- CN106066306A CN106066306A CN201610502561.0A CN201610502561A CN106066306A CN 106066306 A CN106066306 A CN 106066306A CN 201610502561 A CN201610502561 A CN 201610502561A CN 106066306 A CN106066306 A CN 106066306A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/1702—Systems in which incident light is modified in accordance with the properties of the material investigated with opto-acoustic detection, e.g. for gases or analysing solids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/061—Sources
- G01N2201/06113—Coherent sources; lasers
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- Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)
Abstract
This application discloses a kind of photoacoustic microscope system, LASER Light Source is for emission pulse laser and forms line polarized light after polaroid, after shaping beam path alignment expands, it is incident on spatial light modulator surface, pulsed light after being modulated irradiates tissue, the photoacoustic signal being positioned at the tissue other end receives device reception photoacoustic signal, acoustical signal is converted to the signal of telecommunication, data acquisition and procession device gathers the described signal of telecommunication and preserves data, is obtained 3-D view and the cross sectional image of tissue by three-dimensional reconstruction.In the detailed description of the invention of the application, owing to light path introducing spatial light modulator, optics depth of focus can be dramatically increased, thus realize big depth of focus, high-resolution photoacoustic imaging.The application produces bessel beam without using axicon, the problem solving the loss of axicon luminous flux, laser irradiation without short twice interval eliminates the impact that bessel beam secondary lobe brings, simplify design and the component costs of experimental provision, it also avoid high-energy thermal relaxation effect simultaneously and irradiate the injury to biological tissue.
Description
Technical field
The application relates to photoacoustic imaging field, particularly relates to a kind of photoacoustic microscope system.
Background technology
Photoacoustic imaging based on the optical absorption characteristics light that organically blended excites and two kinds of means of acoustic detection.In biological tissue
Coloring matter is converted into heat energy after absorbing pulse laser, due to transient heat buoyancy effect, outwards discharges broadband ultrasonic wave (i.e. optoacoustic
Signal), the optical absorption characteristics of tissue can be measured by detection photoacoustic signal.The optical resolution optoacoustic developed rapidly in recent years
Lateral resolution is promoted to micron dimension by microscope, it is possible in body high-precision imaging blood microcirculqtory system, including at different levels
Main blood vessel, blood capillary, the most single red blood cell.By transition time of photoacoustic signal resolve residing for coloring matter deep
Degree position, optical resolution photoacoustic microscope only needs sample is carried out two dimensional surface scanning, can obtain the three-dimensional microcosmic of tissue
Structural images.But, traditional optical resolution photoacoustic microscope uses Gaussian-shaped beam, is limited by Rayleigh range, and optics gathers
Depth of focus degree is less.The object lens focus excitation beam that common optical resolution photoacoustic microscope uses numerical aperture to be 0.1, can be real
Now it is about the lateral resolution of 6 microns, but keeps the depth of focus of this resolution to be only 100 microns.When imaging region surpasses
Going out this depth bounds, the lateral resolution of system will reduce rapidly.
Existing optical resolution photoacoustic microscope uses object lens to realize the focusing of excitation beam, consequent Gauss light
Bundle has shorter depth of focus, causes imaging resolution only to keep consistent (generally less than 100 microns) in smaller depth region.
Although the bessel beam that cone prism produces has bigger depth of focus, but light beam secondary lobe has had a strong impact on resolution and figure picture element
Amount.The thermal relaxation effect of generation is excited, it is possible to weaken bessel beam to a certain extent by the continuous light of short time interval
The impact of secondary lobe.But, light beam secondary lobe invalid in the method is by large losses light energy, and repeatedly laser irradiation significantly prolongs
Grow imaging time, the photic damage of tissue can have been caused simultaneously.Additionally, the generation of short interval exciting light typically requires two laser
Device, adds complexity and the cost of imaging system.
Summary of the invention
The application to solve the technical problem that being for the deficiencies in the prior art, it is provided that a kind of photoacoustic microscope system.
The application to solve the technical problem that and to solve by the following technical programs:
A kind of photoacoustic microscope system, receives device and data including LASER Light Source, polaroid, shaping light path, photoacoustic signal
Gathering and processing means, also include spatial light modulator, described LASER Light Source is for emission pulse laser and through described polaroid
Rear formation line polarized light, after described shaping beam path alignment expands, is incident on described spatial light modulator surface, after being modulated
Pulsed light irradiate tissue, be positioned at the tissue other end photoacoustic signal receive device reception photoacoustic signal, described acoustical signal is turned
Being changed to the signal of telecommunication, described data acquisition and procession device gathers the described signal of telecommunication and preserves data, obtains group by three-dimensional reconstruction
The 3-D view knitted and cross sectional image.
Described data acquisition and procession device includes controller and data collecting card, and described controller controls described data acquisition
The electric impulse signal that truck exports using described LASER Light Source carries out data acquisition as synchronizing signal to the described signal of telecommunication.
Described controller is integrated on PC or graphics workstation, and described data collecting card is integrated in PC or figure
On work station.
Described controller is individual devices, and described data collecting card is integrated on PC or graphics workstation.
Also include that three-D displacement device, described three-D displacement device make light beam that tissue is carried out two dimensional surface scanning, with
Obtain the 2-D data required for 3-D view storehouse.
Described spatial light modulator includes phase type spatial light modulator.
Described photoacoustic signal receives device and includes ultrasonic probe.
Also include the signal amplifier for amplifying the described signal of telecommunication.
Formed the device of described shaping light path include the first collimator and extender device, reflecting mirror, the second collimator and extender device and
Condenser lens, described line polarized light, after described first collimator and extender device collimator and extender, is irradiated on described reflecting mirror, warp
Described reflecting mirror reflexes to described spatial light modulator, then after described second collimator and extender device carries out collimator and extender, logical
Cross described condenser lens and be irradiated to tissue.
The device forming described shaping light path also includes the 3rd collimator and extender device, the light warp being irradiated on described reflecting mirror
Cross to reenter after described 3rd collimator and extender device carries out collimator and extender and be incident upon described spatial light modulator surface.
Owing to have employed above technical scheme, make what the application possessed to have the beneficial effects that:
In the detailed description of the invention of the application, owing to light path introducing spatial light modulator, the wavefront focusing on light beam is carried out
Modulation, is equivalent to add one layer of optical mask on the principal plane of condenser lens, and system has reached higher logical light rate, sufficiently
Make use of light energy, be effectively improved signal to noise ratio, optics depth of focus can be dramatically increased, thus realize big depth of focus, high-resolution
Photoacoustic imaging.The application produces bessel beam without using axicon, thus solves the problem of axicon luminous flux loss,
The application excites without short interval continuous light simultaneously, simplifies design and the component costs of experimental provision, it also avoid simultaneously
High-energy thermal relaxation effect irradiates the injury to biological tissue.
Accompanying drawing explanation
Fig. 1 (a) is the binary optical image of non-modulated；
Fig. 1 (b) is the binary optical image after spatial light modulator is modulated；
Fig. 2 (a) is the sectional view of the beam focus without modulation；
Fig. 2 (b) is the sectional view of the beam focus after spatial light modulator is modulated；
Fig. 3 is the application system structural representation in one embodiment；
Fig. 4 is the application system structural representation in another embodiment.
Detailed description of the invention
Combine accompanying drawing below by detailed description of the invention the present invention is described in further detail.
The application utilizes LCD space light modulator modulated incident light beam wavefront, and then is obviously improved the optics of imaging depth of focus
Resolution photoacoustic microscope.LCD space light modulator is the core component realizing Beam Wave-Front shaping, will be as shown in Fig. 1 (b)
Binary picture be loaded on LCD space light modulator, cause the change of liquid crystal molecule optical property, make to be incident to spatial light
The Beam Wave-Front generation respective change of modulator surface.In system light path, photo-acoustic excitation light beam is incident to spatial light modulator
Surface, loads on the specific light pattern of spatial light modulator (as Fig. 1 (b) will affect the wavefront distribution of this light beam, through micro-thing
After mirror focuses on, form the light spatial distribution as shown in Fig. 2 (b).Fig. 2 (a) figure is the y-z cross section loading Fig. 1 (a) beam focus afterwards
Figure, Rayleigh range is the shortest；Fig. 2 (b) figure is the y-z sectional view loading Fig. 1 (b) beam focus afterwards, can significantly observe auspicious
The stretching of profit length, while longitudinal length is substantially improved, does not lose the lateral dimension of light beam.Obviously, with non-modulated
Optical focus compare (as shown in Fig. 1 (a) and Fig. 2 (a)), this scheme improves the depth of focus of incidence excitation beam, makes optoacoustic
Imaging transverse resolution keeps unanimously, being conducive to improving image quality in bigger depth bounds.
As shown in Figure 3, Figure 4, the photoacoustic microscope system of the application, its a kind of embodiment, including LASER Light Source 100, polarization
Sheet 200, shaping light path 300, photoacoustic signal receive device 400, data acquisition and procession device 500 and spatial light modulator 600.
LASER Light Source 100 is for emission pulse laser and forms line polarized light after polaroid 200, expands through shaping light path 300 collimation
Shu Hou, is incident on spatial light modulator 600 surface, and the pulsed light after being modulated irradiates tissue, is positioned at the optoacoustic of the tissue other end
Signal receiving device 400 receives photoacoustic signal, and acoustical signal is converted to the signal of telecommunication, and data acquisition and procession device 500 gathers electricity
Signal also preserves data, is obtained 3-D view and the cross sectional image of tissue by three-dimensional reconstruction.In one embodiment,
The spatial light modulator of the application includes phase type spatial light modulator.Photoacoustic signal receives device 400 can include ultrasonic spy
Head.
Data acquisition and procession device includes controller and data collecting card, and controller controls data collecting card with laser light
The electric impulse signal of source output carries out data acquisition as synchronizing signal to the signal of telecommunication.In one embodiment, controller can
To be integrated on PC or graphics workstation, data collecting card is integrated on PC or graphics workstation.Real at another kind
Executing in mode, controller is individual devices, and data collecting card can be integrated on PC or graphics workstation.
The photoacoustic microscope system of the application, it is also possible to include that three-D displacement device 700, three-D displacement device 700 make
Light beam carries out two dimensional surface scanning to tissue, to obtain the 2-D data required for 3-D view storehouse.
In one embodiment, the photoacoustic microscope system of the application, it is also possible to include signal amplifier 800, signal
Amplifier 800 receives the signal of telecommunication of device 400 conversion for amplifying photoacoustic signal.
The device forming shaping light path 300 includes that the first collimator and extender device 310, reflecting mirror the 320, second collimator and extender fill
Put 330 and condenser lens 340.Line polarized light, after the first collimator and extender device 310 collimator and extender, is irradiated to reflecting mirror 320
On, it is reflected mirror 320 and reflexes to described spatial light modulator, then after the second collimator and extender device 330 carries out collimator and extender,
It is irradiated to tissue by condenser lens 340.Collimator and extender device generally comprises condenser lens and collimating lens, and condenser lens is used
In focusing on light beam, collimating lens is for by the light beam-expanding collimation of outgoing.
In one embodiment, the device forming shaping light path 300 can also include that the 3rd collimator and extender device (is schemed not
Show), the light being irradiated on reflecting mirror 320 reenters after described 3rd collimator and extender device carries out collimator and extender and is incident upon spatial light
Manipulator 600 surface.In another embodiment, the device forming shaping light path 300 can also include pinhole filter
350, line polarized light, after the first collimator and extender device 310 collimator and extender, can filter out light beam by pinhole filter 350
After the veiling glare at edge, then it is irradiated on reflecting mirror 320.
Above content is to combine specific embodiment further description made for the present invention, it is impossible to assert this
Bright being embodied as is confined to these explanations.For general technical staff of the technical field of the invention, do not taking off
On the premise of present inventive concept, it is also possible to make some simple deduction or replace.
Claims (10)
1. a photoacoustic microscope system, receives device and number including LASER Light Source, polaroid, shaping light path, photoacoustic signal
According to gathering and processing means, it is characterised in that also include spatial light modulator, described LASER Light Source is for emission pulse laser also
After described polaroid, form line polarized light, after described shaping beam path alignment expands, be incident on described spatial light modulator
Surface, the pulsed light after being modulated irradiates tissue, and the photoacoustic signal being positioned at the tissue other end receives device reception photoacoustic signal, will
Described acoustical signal is converted to the signal of telecommunication, and described data acquisition and procession device gathers the described signal of telecommunication and preserves data, by three
Dimension rebuilds 3-D view and the cross sectional image obtaining tissue.
2. photoacoustic microscope system as claimed in claim 1, it is characterised in that described data acquisition and procession device includes control
Device processed and data collecting card, described controller controls the electric impulse signal work that described data collecting card exports with described LASER Light Source
For synchronizing signal, the described signal of telecommunication is carried out data acquisition.
3. photoacoustic microscope system as claimed in claim 2, it is characterised in that described controller is integrated in PC or figure
On work station, described data collecting card is integrated on PC or graphics workstation.
4. photoacoustic microscope system as claimed in claim 2, it is characterised in that described controller is individual devices, described number
It is integrated on PC or graphics workstation according to capture card.
5. photoacoustic microscope system as claimed in claim 1, it is characterised in that also include three-D displacement device, described three-dimensional
Gearshift makes light beam that tissue is carried out two dimensional surface scanning, to obtain the 2-D data required for 3-D view storehouse.
6. photoacoustic microscope system as claimed in claim 1, it is characterised in that described spatial light modulator includes that phase type is empty
Between photomodulator.
7. photoacoustic microscope system as claimed in claim 1, it is characterised in that described photoacoustic signal receives device and includes ultrasonic
Probe.
8. photoacoustic microscope system as claimed in claim 7, it is characterised in that also include the letter for amplifying the described signal of telecommunication
Number amplifier.
9. the photoacoustic microscope system as according to any one of claim 1 to 8, it is characterised in that form described shaping light path
Device include the first collimator and extender device, reflecting mirror, the second collimator and extender device and condenser lens, described line polarized light passes through
After described first collimator and extender device collimator and extender, it is irradiated on described reflecting mirror, reflexes to described space through described reflecting mirror
Photomodulator, then after described second collimator and extender device carries out collimator and extender, it is irradiated to tissue by described condenser lens
On.
10. photoacoustic microscope system as claimed in claim 9, it is characterised in that the device forming described shaping light path also wraps
Including the 3rd collimator and extender device, the light being irradiated on described reflecting mirror carries out collimator and extender through described 3rd collimator and extender device
After reenter and be incident upon described spatial light modulator surface.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106510635A (en) * | 2016-11-30 | 2017-03-22 | 江西科技师范大学 | Skin vessel photoacoustic imaging device |
| CN108760645A (en) * | 2018-05-29 | 2018-11-06 | 电子科技大学 | A kind of the photo-thermal effect measuring system and its measurement method of strong scattering material |
| CN109297949A (en) * | 2018-09-19 | 2019-02-01 | 珠海彩晶光谱科技有限公司 | The tumour cell detection method and device of micro-imaging combination transmission Raman spectrum |
| WO2019091291A1 (en) * | 2017-11-07 | 2019-05-16 | 华南理工大学 | Method for realizing arbitrary ultrasonic field |
| CN112986140A (en) * | 2019-12-17 | 2021-06-18 | 宁波大学 | Time-resolved imaging system suitable for laser beam shaping and imaging method thereof |
| CN113397488A (en) * | 2021-06-17 | 2021-09-17 | 湖南大学 | Scheme for carrying out quantum correlation imaging on biological tissue based on photoacoustic conversion |
| CN116026764A (en) * | 2023-03-30 | 2023-04-28 | 之江实验室 | Photoacoustic microscopic imaging system and method based on self-focusing light beam |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106510635A (en) * | 2016-11-30 | 2017-03-22 | 江西科技师范大学 | Skin vessel photoacoustic imaging device |
| WO2019091291A1 (en) * | 2017-11-07 | 2019-05-16 | 华南理工大学 | Method for realizing arbitrary ultrasonic field |
| CN108760645A (en) * | 2018-05-29 | 2018-11-06 | 电子科技大学 | A kind of the photo-thermal effect measuring system and its measurement method of strong scattering material |
| CN108760645B (en) * | 2018-05-29 | 2021-07-16 | 电子科技大学 | Photothermal effect measuring system and method for strong scattering material |
| CN109297949A (en) * | 2018-09-19 | 2019-02-01 | 珠海彩晶光谱科技有限公司 | The tumour cell detection method and device of micro-imaging combination transmission Raman spectrum |
| CN109297949B (en) * | 2018-09-19 | 2024-04-05 | 上海镭立激光科技有限公司 | Tumor cell detection method and device by combining microscopic image with transmission Raman spectrum |
| CN112986140A (en) * | 2019-12-17 | 2021-06-18 | 宁波大学 | Time-resolved imaging system suitable for laser beam shaping and imaging method thereof |
| CN113397488A (en) * | 2021-06-17 | 2021-09-17 | 湖南大学 | Scheme for carrying out quantum correlation imaging on biological tissue based on photoacoustic conversion |
| CN116026764A (en) * | 2023-03-30 | 2023-04-28 | 之江实验室 | Photoacoustic microscopic imaging system and method based on self-focusing light beam |
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Application publication date: 20161102 |