CN106053793A - Immunochromatographic test paper card for rapid detection of chylemia and preparation method thereof - Google Patents
Immunochromatographic test paper card for rapid detection of chylemia and preparation method thereof Download PDFInfo
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Abstract
The invention relates to an immunochromatographic test paper card for rapid detection of chylemia and a preparation method thereof. The detection test paper card comprises a colloidal gold test strip and a card slot, wherein the colloidal gold test strip mainly comprises a support plate, an NC film, a gold-labeled antibody pad, a sample pad and a water absorbing pad, and the NC film is arranged in the middle of the support plate; the gold-labeled antibody pad and the sample pad are pasted on the support plate at one side of the NC plate, and the water absorbing pad is pasted on the support plate at the other side of the NC plate; a detection line containing a polyclonal antibody of apoB and a quality control line containing a secondary antibody are arranged in the middle of the NC film. The test paper card has the advantages of rapid detection, high accuracy, strong specificity and the like, does not need to use an instrument and equipment, and is convenient to use. The test paper card for rapid detection of chylemia prepared by the invention can rapidly screen chylemia for blood donors before blood donation, thereby protecting the blood resources provided by the blood donors to a great extent, and also saving the relevant detection expense and the like.
Description
Technical field
The invention belongs to clinical hematology detection, particularly relate to quickly detect colloidal gold test paper card and the preparation side of chylemia
Method.
Background technology
Chylemia or title lipidemia, post prandial fat is through after little intestinal digestion, absorbing, and the Chylomicron becoming tiny enters blood
Liquid, Chylomicron many to a certain extent time, serum is just become milky thick liquid by as clear as crystal weak yellow liquid.Blood
Because of containing a large amount of triglyceride in slurry, and patient can not be defeated by.Triglyceride is the independent hazard factor of cardiovascular disease.
The most domestic when not taking a blood sample for street corner, the way of content of triglyceride is quickly detected with peripheral blood.Therefore cause annual
Scrap substantial amounts of lipidemia, cause the huge waste of blood resource.
Apolipoprotein (apolipoprotein, apo) is the protein in plasma lipoprotein, and its basic function is delivery fat
Class.
The difference that apoB forms due to aminoacid, can be divided into following subclass: apoB48 and apoB100.ApoB48 is chyle
One of apolipoprotein of microgranule (CM)；ApoB100 is very low density lipoprotein (VLDL) (VLDL) and the load fat of low density lipoprotein, LDL (LDL)
One of albumen.
Apo B-48 (apoB-48) is the lipoprotein uniquely secreted by small intestinal (people), after it is combined with triglyceride
Generate Chylomicron (chylomicronsCMs) and residue thereof.ApoB-48 in CM is structural protein, by apoB100mRNA
Special editor and obtain.ApoB48 has 2153 aminoacid.After it is esterified by neutral fat, in triglyceride transport protein
Under the assistance of (triglyceridetransferprotein (MTP)), generate the CM granule at initial stage.Unconjugated apoB48 stands
I.e. degrade.Different from liver secretion very low density lipoprotein (VLDL) (VLDL), the apoB48 that degrades in enterocyte is not its main regulation and control
Mechanism, when lipid lacks, the apoB48 of enterocyte secretion is a kind of high density lipoprotein.In serum, apoB48's is dense
Degree can represent the granule number of CM and CM residue.Available apoB48 follows the trail of the metabolic process of intestinal secretion CM.
Current commonly used centrifuging detection chylemia, but during because using centrifuge, the power of needs and space requirement, only
Can use at fixing blood sampling point, mobile blood-collecting carriage cannot use the method；When using centrifuging, blood donor 2 ~ 3ml need to be gathered quiet
Arteries and veins blood screens, and the time taking around 10 ~ 15 minutes is centrifuged judgement；Although centrifuging testing result is accurate, but
It is because its use is limited, it is impossible to use it to chylemia rapid screening.
ELISA, molecular biology experiment technology are applicable to laboratory and use, and are not suitable for during the blood sampling of street corner using.Immunity
Chromatography colloidal gold technique is the optimum selection of rapid clinical detection.Gold colloidal detection chylemia, it is not necessary to use extra equipment,
Speed is fast, highly sensitive, when being particularly well-suited to street corner blood sampling, screens blood donor.The invention belongs to domestic initiation, at present
The immunity colloidal gold test paper strip of the quickly detection for chylemia is there is no on market.
Summary of the invention
When it is an object of the invention to as solving to take a blood sample in street corner, blood donor carrying out rapid screening, superseded chylemia is donated blood
Person, saves valuable blood resource.
The present invention solves above-mentioned technical problem, the technical scheme is that
The first aspect of the invention is, the immune chromatography test card of a kind of quick detection chylemia, uses colloid gold immune
Chromatography and all methods derived by colloidal gold immunity chromatography, preferably use double antibody in colloidal gold immunity chromatography
Sandwich assay detection antigen.
The described immune colloid gold test paper card quickly detecting chylemia, including colloidal gold strip and draw-in groove, this draw-in groove
There is the build-in cavities of an accommodating colloidal gold strip, and on this draw-in groove, be provided with observation panel and well；This gold colloidal
Test strips is mainly made up of, at this support plate, NC film (i.e. nitrocellulose filter), gold labeling antibody pad, sample pad and adsorptive pads
Hold the side of plate to pass through bond samples pad, gold labeling antibody pad, NC film and adsorptive pads successively；Middle part at this NC film
It is provided with a detection line containing apolipoprotein polyclonal antibody and one containing two anti-nature controlling lines, this detection line and nature controlling line
Interval is arranged, and it is adjacent with gold labeling antibody pad to detect line；This colloidal gold strip is arranged in the build-in cavities of draw-in groove, and makes
The detection line of this observation panel correspondence colloidal gold strip and nature controlling line, well counter sample pad.
In described gold labeling antibody pad, gold labeling antibody is mouse-anti human apolipoprotein b 48 monoclonal antibody, the anti-human apolipoprotein of rabbit
At least one in B48 monoclonal antibody waits, preferred mouse-anti human apolipoprotein b 48 monoclonal antibody；
Apolipoprotein B polyclonal antibody in detection line includes rabbit anti-human apolipoprotein B polyclonal antibody, rabbit anti-Mus apolipoprotein
In B polyclonal antibody, goat-anti human apolipoprotein b polyclonal antibody, sheep anti mouse apolipoprotein B polyclonal antibody, at least one, excellent
Select rabbit anti-Mus apolipoprotein B polyclonal antibody；
In nature controlling line two are anti-includes that rabbit anti-mouse igg two is anti-, sheep anti-mouse igg two is anti-, rabbit anti-human igg two is anti-, goat anti-human igg two resists
In at least one, preferably rabbit anti-mouse igg two resists；
The second aspect of the invention is, quickly the preparation method of the immune chromatography test card of detection chylemia, including following
Step:
1) preparation of line is detected: use gold spraying instrument, with being coated buffer dilution rabbit anti-Mus apolipoprotein B polyclonal antibody, at NC
Film detects line position line, is detection line, dries standby；The described buffer that is coated is through 0.45 μm membrane filtration
Being coated buffer, buffer can be carbonate buffer solution, phosphate buffer, HEPES buffer, in Tris-HCl etc. extremely
Few one, preferably carbonate buffer solution, its concentration range can be 0.01 ~ 0.5M, preferably 0.05 ~ 0.1M, the scope of its pH value
It is 8.0 ~ 10.0, preferably 9.2 ~ 9.6；
The preparation of nature controlling line: use gold spraying instrument, resists with being coated buffer dilution rabbit anti-mouse igg two, puts down with detection line on NC film
The position line of row, is spaced 3 ~ 7mm, is nature controlling line, and the described buffer that is coated is being coated through 0.45 μm membrane filtration
Buffer, buffer can be carbonate buffer solution, phosphate buffer, HEPES buffer, at least in Tris-HCl etc.
Kind, preferably carbonate buffer solution, its concentration range can be 0.01 ~ 0.5M, preferably 0.05 ~ 0.1M, and its pH value is in the range of 8.0
~ 10.0, preferably 9.2 ~ 9.6；
Wherein, in step (1), the line width of detection line and nature controlling line is 1mm, and two lines, at a distance of 4mm, are positioned at the centre of film, away from NC film
Back gauge 6 ~ 7mm；
NC film assembles preparation: dry detection line and nature controlling line, obtains containing detection line and the NC film of nature controlling line；With closing working solution
The NC film of preparation is dried in room temperature 72h, seals up for safekeeping standby；Described closing working solution is 2 ~ 4% BSA, 2 ~ 4% defatted milk powder and
Carbonate buffer solution mixes, and obtains closing working solution with 0.45 μm membrane filtration, and wherein the concentration range of carbonate buffer solution can
Be 0.01 ~ 0.5M, preferably 0.01 ~ 0.1M, its pH value in the range of 6.0 ~ 8.0, preferably 6.5 ~ 7.5.
2) preparation coating gold labeling antibody pad
With containing 1 ~ 3% BSA, 0.1 ~ 0.2% sodium azide, 5 ~ 15% sucrose, 5 ~ 10% trehaloses, Tris-HCl buffer dilution gold mark
Mouse-anti human apolipoprotein b 48 monoclonal antibody, by gold labeling antibody solution gold spraying instrument, be uniformly sprayed on glass fibre cotton, 56
DEG C dry 1h, adds after desiccant vacuum seals, and room temperature preservation is standby.
The wherein preparation method of gold colloidal: the preparation of colloidal gold solution has many kind methods, most common of which is chemistry
Reducing process, ultimate principle is to add a certain amount of reducing agent in certain density gold solution to make gold ion become gold atom.Mesh
Front conventional reducing agent has: white phosphorus, ethanol, hydrogen peroxide, sodium borohydride, ascorbic acid, sodium citrate, tannic acid etc..Preferably lemon
Lemon acid sodium, as reducing agent, uses reduction of sodium citrate gold chloride, the colloid gold particle of preparation 30 ~ 40nm.
3) chylemia quick detection kit is assembled
The NC film scribbling detection line and nature controlling line is pasted the middle part at support plate, pastes successively in the side of NC film containing gold mark
The golden labeling antibody pad of antibody and sample pad, the opposite side at NC film is pasted adsorptive pads, is obtained test paper plate, cut into by test paper plate
The test strips of different in width, is placed in test strips in draw-in groove, is chylemia quick detection test paper card.
The application of chylemia quick detection test paper card and judgement: when the blood donor donating blood street corner carries out primary dcreening operation, collection is offered
The peripheral blood 40 μ L of blood person, drips in the well of this test card, all occurs in detection line, the nature controlling line position of test card
During red stripes, chylemia test positive；When only a red stripes occurs in nature controlling line, for negative findings；At test card
It is null result that nature controlling line does not occurs during red stripes.
Beneficial effects of the present invention:
Chylemia or title lipidemia, because containing a large amount of triglyceride in blood plasma, and can not be defeated by patient.The most not
Have when taking a blood sample for street corner, quickly detect the way of chylemia with peripheral blood.Therefore cause scrapping substantial amounts of chylemia every year, make
Become the huge waste of blood resource.
For the qualification of chylemia, generally use centrifugal method.After gathering 2 ~ 3ml blood, static 5 minutes, then
3000g is centrifuged 5 ~ 10 minutes, and blood can be made to be layered, and observes by the naked eye and identify the limpid degree of serum layer, it is judged that blood is
No for chylemia.But when the method is not suitable for street corner blood sampling, blood donor being carried out chylemia screening, reason is as follows: 1. street corner
Screening before donating blood, general employing peripheral blood detects, it is impossible to obtain 2 ~ 3ml blood sample；2. on mobile blood-collecting carriage, due to
Narrow space, is short of power, it is impossible to use centrifuge；3. screen before donate blood in street corner, typically require to complete within 2 minutes to own
Detection, the overlong time of centrifuging.
For above-mentioned situation, the present invention is that domestic initiation prepares chylemia quick detection test paper card, and this test card has inspection
Survey the advantages such as quick, accuracy height, high specificity, and need not use other instrument and equipments, easy to use.Prepared by the present invention
Chylemia quick detection test paper card, quickly can carry out chylemia screening to blood donor before donating blood, use 40 microlitre peripheral bloods, energy
Enough in 1 minute, blood donor is carried out chylemia screening, quick and precisely efficient detection chylemia, rate of accuracy reached to more than 98%, from
And the blood resource of effectively save blood donor, reduce the Blood discarding rate of blood station largely, can scrapping chylemia
Rate is reduced to 0 ~ 0.1% from 1 ~ 1.5%, can either protect the blood resource that blood donor provides, moreover it is possible to for the inspection that blood station saving is relevant
Survey, store, the expense such as return.
Accompanying drawing explanation
Fig. 1 is the structural representation of colloidal gold strip of the present invention
Fig. 2 is the perspective view of buckle type draw-in groove
Fig. 3 is the testing result schematic diagram of the colloidal gold test paper card using the present invention
Description of reference numerals: 1, sample pad；2, gold labeling antibody pad；3, NC film (nitrocellulose filter)；4, detection line, 5, Quality Control
Line；6, adsorptive pads；7, support plate；8, draw-in groove；9 observation panels；10, well；11, build-in cavities.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment 1
Seeing Fig. 1 and Fig. 2, the immune chromatography test card of a kind of quick detection chylemia of the present invention, including colloidal gold strip A
With buckle type draw-in groove 8, this buckle type draw-in groove 8 has the build-in cavities 11 of an accommodating colloidal gold strip A, and at this buckle type
Observation panel 9 and well 10 it is provided with on draw-in groove 8；This colloidal gold strip A is mainly by support plate 7, NC film 3, gold labeling antibody pad
2, sample pad 1 and adsorptive pads 6 form, and pass through in the side of this support plate 7 bond samples pad 1, gold mark successively resist
Body pad 2, NC film 3 and adsorptive pads 6；A detection line 4 containing apolipoprotein B polyclonal antibody it is provided with at the middle part of this NC film 3
Arrange containing the anti-nature controlling line 5 of IgG bis-, this detection line 4 and nature controlling line 5 interval with one, and detect line 4 and gold labeling antibody pad
2 is adjacent；This colloidal gold strip A is arranged in the build-in cavities 11 of buckle type draw-in groove 8, and makes the corresponding gold colloidal of this observation panel 9
The region of the detection line 4 NC film 3 corresponding with nature controlling line 5(of test strips A), the sample pad 1 of the corresponding colloidal gold strip of well 10.
In described gold labeling antibody pad 2, gold labeling antibody is mouse-anti human apolipoprotein b 48 monoclonal antibody；Described NC film 3 detects line 4
Multi-resistance be rabbit anti-Mus apolipoprotein B polyclonal antibody, the two of nature controlling line 5 resist and resist for rabbit anti-mouse igg two.
Embodiment 2
The preparation method of chylemia quick detection test paper card, comprises the steps:
1) NC film 3 is prepared
The preparation of detection line 4: use gold spraying instrument, with being coated buffer dilution carrier protein B polyclonal antibody, detects in NC film
Line position is rule, and is detection line, dries standby；The described buffer that is coated is the 0.05M through 0.45 μm membrane filtration
The carbonate buffer solution of pH9.6；
The preparation of nature controlling line 5: use gold spraying instrument, resists with being coated buffer dilution rabbit anti-mouse igg two, puts down with detection line on NC film
The position line of row, is spaced 5mm, is nature controlling line, and the described buffer that is coated is the 0.05M through 0.45 μm membrane filtration
The carbonate buffer solution of pH9.6；
NC film assembles preparation: dry detection line and nature controlling line, obtains containing detection line and the NC film of nature controlling line；With closing working solution
The NC film of preparation is dried in room temperature 72h, seals up for safekeeping standby；Described closing working solution is 2.5%BSA, 2% defatted milk powder and
Obtain closing working solution with 0.45 μm membrane filtration after the carbonate buffer solution mixing of 0.01M, pH7.0.
2) preparation coating gold labeling antibody pad 2
With containing 1.5%BSA, 0.1% sodium azide, 8% sucrose, 5% trehalose, the dilution buffer dilution of 0.5MTris-HCl, pH8.5
The monoclonal antibody of gold target apolipoprotein B；By gold labeling antibody solution gold spraying instrument, uniformly it is sprayed on glass fibre cotton, 56 DEG C of dry 1h,
After adding the sealing of desiccant vacuum, room temperature preservation is standby.
3) chylemia quick detection test paper card is assembled
The NC film scribbling detection line and nature controlling line is pasted the middle part at support plate, pastes gold labeling antibody in the side of NC film successively
Pad and sample pad, the opposite side at NC film is pasted absorption pad, is obtained test paper plate, test paper plate cuts into the reagent paper of different in width
Bar, is placed in test strips in draw-in groove, is chylemia quick detection test paper card.
Embodiment 3
After preparing chylemia quick detection test paper card according to the method for embodiment 1 and embodiment 2, use by the following method.
1) the using and judge of chylemia quick detection test paper card
When the blood donor donating blood street corner carries out primary dcreening operation, gather terminal blood 40 microlitre of blood donor, drip in this test card
In sample pad 1 (in Fig. 1 at arrow B), after dropping blood, serum will carry out siphon advance, blood by arrow C direction in Fig. 1
ApoB48 in Qing first can be with the apoB48 monoclonal antibody specific bond being marked with gold colloidal on gold mark pad 2, and with blood
Travelling forward clearly, when arriving detection line 4 position, apoB48 can be fixed in the apoB48 polyclonal antibody of detection line 4 and be caught
Obtaining, when there being abundant apoB48 to be captured, the gold colloidal that it connects shows red because of gathering.The mark of uncombined apoB48
Note has the apoB48 monoclonal antibody of gold colloidal to may proceed to travel forward until nature controlling line 5, is fixed with IgG bis-on nature controlling line 5
Anti-, the two anti-apoB48 monoclonal antibodies being marked with gold colloidal of all of uncombined apoB48 that will capture, and aobvious redness.
When the detection line 4 of test card, nature controlling line 5 position all red stripes occur, chylemia test positive is (such as figure
3b)；When only a red stripes occurs in nature controlling line 5, for negative findings (such as Fig. 3 a)；Nature controlling line 5 at test card does not occurs red
It is null result (such as Fig. 3 c and Fig. 3 d) during vitta band.
2) the actual application on the blood-collecting car of street corner
Four mobile blood-collecting carriages of certain provincial Blood Center the most at home, two fixing blood sampling points, carry out respectively one month by a definite date
Chylemia screening.One blood-collecting car or a blood sampling point, can gather the blood of about 2000 blood donors, Qi Zhong great for one month
About 20 ~ 30 blood donors are chylemia, and scrappage is 1 ~ 1.5%.
In using colloidal gold test paper card screening one month of chylemia, 4 mobile blood-collecting carriages gather respectively 2,1,0
Name, 1 chylemia blood donor, scrappage is respectively 0.1%, 0.05%, 0,0.05%.Data show, select this test card screening breast
After rotten blood, Blood discarding rate can be reduced to 0 ~ 0.1% from 1 ~ 1.5%, save a large amount of blood resource.
On two fixing blood sampling points, compare with colloidal gold method by centrifuging.Two blood sampling points, one month it
In, gather 4057 blood donors altogether.In 4057 blood donors, use centrifuging as comparison, detect chylemia blood donor
It is 47, wherein 3 entitled slight chylemia blood donor；Colloidal gold fast detecting test paper block-regulations detection chylemia blood donor is 44
Name, accuracy rate is 99.9%.
The colloid gold test paper block-regulations screening chylemia of the present invention, is not limited by place and equipment, is used peripheral blood, 1
The screening of chylemia is completed in minute.The colloidal gold strip of the application of the invention, greatly reduces the scrappage of chylemia,
Save blood resource greatly.
Above-described embodiment is only technology design and the feature of the explanation present invention, can not limit the protection model of the present invention with this
Enclosing, all modifications and variations made according to spirit of the invention, within being encompassed by protection scope of the present invention.
Claims (8)
1. the immune chromatography test card of a quick detection chylemia, it is characterised in that the target spot of detection chylemia is to carry fat egg
White B.
The immune chromatography test card of a kind of quick detection chylemia the most according to claim 1, it is characterised in that detection chylemia
Target spot be apolipoprotein B48.
A kind of immune chromatography test card detecting chylemia the most according to claim 1 and 2, it is characterised in that use glue
Body gold immunochromatographic method and all methods derived by colloidal gold immunity chromatography.
A kind of immune chromatography test card detecting chylemia the most according to claim 3, it is characterised in that described colloid
Gold immunochromatographic method uses double antibody sandwich method.
The immune chromatography test card of a kind of quick detection chylemia the most according to claim 3, it is characterised in that
Including colloidal gold strip and draw-in groove, this draw-in groove has the build-in cavities of an accommodating colloidal gold strip, and at this card
Observation panel and well it is provided with on groove；This colloidal gold strip mainly by support plate, NC film, gold labeling antibody pad, sample pad and
Adsorptive pads forms, and pass through bond samples pad, gold labeling antibody pad, NC film and water suction successively in the side of this support plate
Pad；A detection line drawn with apolipoprotein polyclonal antibody and one it is provided with by the two anti-matter drawn at the middle part of this NC film
Control line, this detection line and nature controlling line interval are arranged, and it is adjacent with gold labeling antibody pad to detect line；This colloidal gold strip is arranged on
In the build-in cavities of draw-in groove, and make detection line and the nature controlling line of this observation panel correspondence colloidal gold strip, well counter sample
Pad.
6. the immune colloid gold test paper card of a quick detection chylemia according to claim 5, it is characterised in that
The preparation method of described detection line comprises the following steps: use gold spraying instrument, carries fat egg with being coated the buffer dilution anti-Mus of rabbit
White B polyclonal antibody, detects line position line in NC film, is detection line, dries standby；The described buffer that is coated is warp
That crosses 0.45 μm membrane filtration is coated buffer, is coated buffer and includes carbonate buffer solution, phosphate buffer, and HEPES delays
Rush liquid, in Tris-HCl at least one, being coated range of buffer concentration is 0.01 ~ 0.5M, and pH value range is 8.0 ~ 10.0, is coated
Buffer is preferably carbonate buffer solution, and being coated buffer preferred concentration range is 0.05 ~ 0.1M, and pH value range is preferably 9.2 ~
9.6；
The preparation method of described nature controlling line comprises the following steps: use gold spraying instrument, with being coated buffer dilution rabbit anti-mouse igg two
Anti-, parallel with detection line on NC film position line, is spaced 3 ~ 7mm, is nature controlling line, and described is coated buffer for passing through
0.45 μm membrane filtration be coated buffer, be coated buffer and include carbonate buffer solution, phosphate buffer, HEPES buffers
Liquid, in Tris-HCl at least one, concentration range is 0.01 ~ 0.5M, and pH value range is 8.0 ~ 10.0, be coated buffer be preferably
Carbonate buffer solution, being coated buffer preferred concentration range is 0.05 ~ 0.1M, and pH value range is preferably 9.2 ~ 9.6；
Wherein, the line width of described detection line and nature controlling line is 1mm, and two lines, at a distance of 4mm, are positioned at the centre of film, away from the limit of NC film
Away from 6 ~ 7mm；
The preparation method of described NC film comprises the following steps: dry detection line and nature controlling line, obtains containing detection line and nature controlling line
NC film；Wherein dry and use closing working solution by the NC film of preparation in room temperature 72h, seal up for safekeeping standby；Described closing working solution is 2
~ 4%BSA, 2 ~ 4% defatted milk powder and carbonate buffer solution mixing, obtain closing working solution, wherein carbonic acid with 0.45 μm membrane filtration
The concentration range of salt buffer is 0.01 ~ 0.5M, and pH value range is 6.0 ~ 8.0, and the concentration range of carbonate buffer solution is preferably
0.01 ~ 0.1M, pH value range preferably from 6.5 ~ 7.5；
The preparation method of described gold labeling antibody pad comprises the following steps: with containing 1.5%BSA, 0.1% sodium azide, 8% sucrose, 5% Sargassum
Sugar, the monoclonal antibody of the dilution buffer dilution gold target apolipoprotein B of 0.5M Tris-HCl, pH8.5；Gold labeling antibody solution is used
Gold spraying instrument, is uniformly sprayed on glass fibre cotton, 56 DEG C of dry 1h, and after adding the sealing of desiccant vacuum, room temperature preservation is standby.
The immune chromatography test card of quick detection chylemia the most according to claim 4, it is characterised in that described gold colloidal
Preparation method be chemical reduction method, wherein reducing agent includes white phosphorus, ethanol, hydrogen peroxide, sodium borohydride, ascorbic acid, lemon
Lemon acid sodium, tannic acid, preferably with reducing agent reduction of sodium citrate gold chloride, the colloid gold particle of preparation 30 ~ 40nm.
The immune chromatography test card of a kind of quick detection chylemia the most according to claim 6, it is characterised in that
Apolipoprotein B polyclonal antibody in described detection line includes that rabbit anti-human apolipoprotein B polyclonal antibody, the anti-Mus of rabbit carry fat
In protein B polyclonal antibody, goat-anti human apolipoprotein b polyclonal antibody, sheep anti mouse apolipoprotein B polyclonal antibody at least one
Kind, preferably rabbit anti-Mus apolipoprotein B polyclonal antibody；
In described nature controlling line two are anti-includes that rabbit anti-mouse igg two is anti-, sheep anti-mouse igg two is anti-, anti-, the goat anti-human igg of rabbit anti-human igg two
Two anti-at least one, preferably the two of nature controlling line resist and resist for rabbit anti-mouse igg two；
Golden labeling antibody in described gold mark pad is apolipoprotein B48 monoclonal antibody, including mouse-anti human apolipoprotein b 48 monoclonal
Antibody, in rabbit anti-human apolipoprotein B48 monoclonal antibody at least one, preferably mouse-anti human apolipoprotein b 48 monoclonal antibody.
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