CN106047988A - A kit for measuring 1,5-anhydroglucitol and a preparing method thereof - Google Patents
A kit for measuring 1,5-anhydroglucitol and a preparing method thereof Download PDFInfo
- Publication number
- CN106047988A CN106047988A CN201610273306.3A CN201610273306A CN106047988A CN 106047988 A CN106047988 A CN 106047988A CN 201610273306 A CN201610273306 A CN 201610273306A CN 106047988 A CN106047988 A CN 106047988A
- Authority
- CN
- China
- Prior art keywords
- mmol
- reagent
- tris buffer
- solvent
- purified water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A kit for measuring 1,5-anhydroglucitol and a preparing method thereof are disclosed. The kit comprises a reagent R1 and a reagent R2 which are independent two liquid components. The reagent R1 comprises a Tris buffer liquid, glucokinase, pyruvate kinase, ascorbic acid oxidase, adenosine triphosphate, phosphoenolpyruvate, 4-aminoantipyrine, MgCl2 and KCl, and a solvent of the reagent R1 is purified water. The reagent R2 comprises a Tris buffer liquid, pyranose oxidase, horse radish peroxidase and 3-hydroxy-2,4,6-triiodobenzoic acid, and a solvent of the reagent R2 is purified water. The preparing method includes mixing a serum sample to be measured with the reagent R1 and the reagent R2, measuring an absorbance difference after a reaction, and calculating the concentration of the 1,5-anhydroglucitol in the sample. The kit and the method have advantages of high accuracy, and the like.
Description
Technical field
The present invention relates to medical science and technological field of biochemistry, a kind of examination measuring 1,5-AG
Agent box and preparation method thereof.
Background technology
1,5-AG (1,5-AG), also known as 1,5-AG or 1-deoxyglucose, is that one has pyrans
The six-carbon monosaccharide of ring structure, is distributed widely in during human body respectively organizes, and in other tissue 1,5-AG content is considerably beyond in blood
Content, because metabolism is slow, therefore blood middle concentration varies less, 1,5-AG (1,5-AG) in blood plasma with non-binding shape
Formula exists, and molecular weight is little, and hydrophilic is strong, can pass freely through glomerular basement membrane, almost all heavily be absorbed at renal tubules, this
Heavily absorb substantially to be drained by urine glucose and suppressed.
In blood plasma, 1,5-anhydroglucose alcohol (1,5-AG) level and glucose, glycolated hemoglobin and fructosamine are in notable negative
Relevant, have data to show, plasma glucose > 8.3mmol/L person, there is high-caliber blood plasma 1,5-AG without exception
(1,5-AG), blood plasma 1,5-anhydroglucose alcohol (1,5-AG) of diabetes patient is substantially less than Healthy People, and diabetics is due to instead
Multiple, lasting increases the excretion of glucose in urine, therefore inhibit renal tubules to 1, the heavily absorption of 5-AG, cause Plasma
Substantially reduce.
Measuring 1,5-AG at present mainly uses full-enzyme method to measure, and mainly has enzymatic measurement and dehydrogenation enzyme process two kinds
Method, but both approaches not only operates complexity, and also owing to having glucose interference, therefore detection accuracy is poor.
Summary of the invention
The operation of existing detection method is complicated, detection accuracy is poor in order to overcome for the technical problem to be solved
Defect, and a kind of test kit measuring 1,5-AG and preparation method thereof is provided.
The technical scheme that the present invention solves above-mentioned technical problem and provides is: the invention discloses a kind of 1,5-of mensuration and is dehydrated Portugal
The test kit of sugar alcohol, including reagent R1 independent of each other and reagent R2 biliquid component, including composition and corresponding content be:
Reagent R1:
Tris buffer 20 ~ 180 mmol/L
Glucokinase 5 ~ 65 g/L
Pyruvate kinase 0.1 ~ 7.0 KU/L
Ascorbic acid oxidase 2.0 ~ 10.0 KU/L
Adenosine triphosphate 0.2 ~ 1.8 mmol/L
Phosphopyruvic acid enolate 1.5 ~ 6.5 mmol/L
4-amino antipyrine 0.2 ~ 1.8 mmol/L
MgCl2 5~25 mmol/L
KCl 10~150 mmol/L
Its solvent is purified water.
Reagent R2:
Tris buffer 20 ~ 180 mmol/L
Pyranose oxidase 50 ~ 150 KU/L
Horseradish peroxidase 0.1 ~ 16 KU/L
3-hydroxyl-2,4,6-Triiodobenzoic acid 0.5 ~ 8.5 mmol/L
Its solvent is purified water.
As preferably, the invention discloses a kind of test kit measuring 1,5-AG, including examination independent of each other
Agent R1 and reagent R2 biliquid component composition, including composition and corresponding content be:
Reagent R1:
Tris buffer 100 mmol/L
Glucokinase 35 g/L
Pyruvate kinase 3.5 KU/L
Ascorbic acid oxidase 6.0 KU/L
Adenosine triphosphate 1.0 mmol/L
Phosphopyruvic acid enolate 4.0 mmol/L
4-amino antipyrine 1.0 mmol/L
MgCl2 15 mmol/L
KCl 80 mmol/L
Its solvent is purified water.
Reagent R2:
Tris buffer 100 mmol/L
Pyranose oxidase 100 KU/L
Horseradish peroxidase 8 KU/L
3-hydroxyl-2,4,6-Triiodobenzoic acid 4.5 mmol/L
Its solvent is purified water.
As preferably, in described reagent R1, the pH value of described Tris buffer is 6～9.
As preferably, in described reagent R2, the pH value of described Tris buffer is 6～9.
As preferably, the invention also discloses preparation method and the use of the test kit of said determination 1,5-AG
Method, comprises the following steps:
A () prepares reagent according to following component content:
Reagent R1:
Tris buffer 20 ~ 180 mmol/L
Glucokinase 5 ~ 65 g/L
Pyruvate kinase 0.1 ~ 7.0 KU/L
Ascorbic acid oxidase 2.0 ~ 10.0 KU/L
Adenosine triphosphate 0.2 ~ 1.8 mmol/L
Phosphopyruvic acid enolate 1.5 ~ 6.5 mmol/L
4-amino antipyrine 0.2 ~ 1.8 mmol/L
MgCl2 5~25 mmol/L
KCl 10~150 mmol/L
Its solvent is purified water.
Reagent R2:
Tris buffer 20 ~ 180 mmol/L
Pyranose oxidase 50 ~ 150 KU/L
Horseradish peroxidase 0.1 ~ 16 KU/L
3-hydroxyl-2,4,6-Triiodobenzoic acid 0.5 ~ 8.5 mmol/L
Its solvent is purified water；
B test serum sample is mixed by () with reagent R1 and reagent R2 so that it is fully react；
C () measures reacted absorbance difference with automatic clinical chemistry analyzer；
D () calculates the concentration of 1,5-anhydroglucose alcohol in sample according to absorbance changing value.
The Cleaning Principle of the present invention is: first process serum sample, through glucokinase and pyruvate kinase with reagent R1
Coupling system makes glucose in serum thoroughly be converted into the G6P not reacted with pyranose oxidase, to remove Fructus Vitis viniferae
The interference effect of sugar, is subsequently adding reagent R2, the most tested 1, and 5-AG is oxidized to 1,5-anhydrofructose and H2O2, the latter is through peppery
Root peroxidase, 4-amino antipyrine and 3-hydroxyl-2,4,6-Triiodobenzoic acid response system effect, produce Trinder
Reaction, occurs chromogenic reaction to carry out colorimetric determination, thus calculates in sample 1, the content of 5-AG.
1,5-anhydroglucose alcohol (1,5-AG) (umol/L)=CS×(umol/L)
In formula: Δ ATThe sample cell absorbance compared with blank tube absorbance
ΔASThe calibration pipe absorbance compared with blank tube absorbance
CSThe concentration of 1,5-AG in calibration solution
Compared with prior art, the present invention has a following advantageous benefits:
Serum 1,5-AG detection is the sensitiveest compared with for other evaluation experimental as the random experiments of screening diabetes, and operation letter
Just, being suitable for clinical laboratory's routine and carry out large-scale detection, serum 1,5-AG detects without as OGTT, needs repeatedly to adopt
Blood detects, and limits without blood sampling, and the method applies glucokinase and pyruvate kinase system response, by Fructus Vitis viniferae
Sugar is converted into the G6P being not involved in reaction, eliminates the interference of endogenous glucose, hence in so that testing result is more
Add accurately.
Detailed description of the invention
Further illustrate below in conjunction with specific embodiment, but the present invention is not limited in these embodiments.
Embodiment 1
The test kit of the present invention includes reagent R1 independent of each other and reagent R2 biliquid component, wherein
Reagent R1:
Tris buffer 100 mmol/L
Glucokinase 35 g/L
Pyruvate kinase 3.5 KU/L
Ascorbic acid oxidase 6.0 KU/L
Adenosine triphosphate 1.0 mmol/L
Phosphopyruvic acid enolate 4.0 mmol/L
4-amino antipyrine 1.0 mmol/L
MgCl2 15 mmol/L
KCl 80 mmol/L
Its solvent is purified water.
Reagent R2:
Tris buffer 100 mmol/L
Pyranose oxidase 100 KU/L
Horseradish peroxidase 8 KU/L
3-hydroxyl-2,4,6-Triiodobenzoic acid 4.5 mmol/L
Its solvent is purified water.
Embodiment 2
The test kit of the present invention includes reagent R1 independent of each other and reagent R2 biliquid component, wherein
Reagent R1:
Tris buffer 110 mmol/L
Glucokinase 65 g/L
Pyruvate kinase 7.0 KU/L
Ascorbic acid oxidase 2.0KU/L
Adenosine triphosphate 0.2 mmol/L
Phosphopyruvic acid enolate 6.5 mmol/L
4-amino antipyrine 0.2 mmol/L
MgCl2 5 mmol/L
KCl 10 mmol/L
Its solvent is purified water.
Reagent R2:
Tris buffer 20mmol/L
Pyranose oxidase 150 KU/L
Horseradish peroxidase 16 KU/L
3-hydroxyl-2,4,6-Triiodobenzoic acid 0.5mmol/L
Its solvent is purified water.
Embodiment 3
The preparation and application of test kit
1, reagent is prepared according to following component content:
Reagent R1:
Tris buffer 100 mmol/L
Glucokinase 35 g/L
Pyruvate kinase 3.5 KU/L
Ascorbic acid oxidase 6.0 KU/L
Adenosine triphosphate 1.0 mmol/L
Phosphopyruvic acid enolate 4.0 mmol/L
4-amino antipyrine 1.0 mmol/L
MgCl2 15 mmol/L
KCl 80 mmol/L
Its solvent is purified water.
Reagent R2:
Tris buffer 100 mmol/L
Pyranose oxidase 100 KU/L
Horseradish peroxidase 8 KU/L
3-hydroxyl-2,4,6-Triiodobenzoic acid 4.5 mmol/L
Its solvent is purified water.
2, automatic clinical chemistry analyzer parameter is arranged
(a) detection temperature: 37 DEG C；
(b) detection wavelength: dominant wavelength 570nm, commplementary wave length 700nm；
(c) response time: 10min, wherein, incubation time 5min, measure immediately after adding reagent R2 and read absorbance
Read absorbance A 2 after A1,5min, calculate absorbance changes delta A=A2-A1；
(d) the Direction of Reaction: positive reaction.
3, detecting step
A () takes 225 μ l reagent R1 and the mixing of 6 μ l test serum samples；
B solution after mixing is hatched 5min under conditions of 37 DEG C by ()；
C () adds 75 μ l reagent R2, measure immediately and read absorbance A 1, reads absorbance A 2 after 5min, calculates absorbance change
Δ A=A2-A1；
D () is according to 1,5-AG(umol/L)=CS ×(umol/L) the dense of 1,5-anhydroglucose alcohol in sample is calculated
Degree.
Embodiment 4
The preparation and application of test kit
1, reagent is prepared according to following component content:
Reagent R1:
Tris buffer 110 mmol/L
Glucokinase 65 g/L
Pyruvate kinase 7.0 KU/L
Ascorbic acid oxidase 2.0KU/L
Adenosine triphosphate 0.2 mmol/L
Phosphopyruvic acid enolate 6.5 mmol/L
4-amino antipyrine 0.2 mmol/L
MgCl2 5 mmol/L
KCl 10 mmol/L
Its solvent is purified water.
Reagent R2:
Tris buffer 20mmol/L
Pyranose oxidase 150 KU/L
Horseradish peroxidase 16 KU/L
3-hydroxyl-2,4,6-Triiodobenzoic acid 0.5mmol/L
Its solvent is purified water.
2, automatic clinical chemistry analyzer parameter is arranged
(a) detection temperature: 37 DEG C；
(b) detection wavelength: dominant wavelength 570nm, commplementary wave length 700nm；
(c) response time: 10min, wherein, incubation time 5min, measure immediately after adding reagent R2 and read absorbance
Read absorbance A 2 after A1,5min, calculate absorbance changes delta A=A2-A1；
(d) the Direction of Reaction: positive reaction.
3, detecting step
A () takes 225 μ l reagent R1 and the mixing of 6 μ l test serum samples；
B solution after mixing is hatched 5min under conditions of 37 DEG C by ()；
C () adds 75 μ l reagent R2, measure immediately and read absorbance A 1, reads absorbance A 2 after 5min, calculates absorbance change
Δ A=A2-A1；
D () is according to 1,5-AG(umol/L)=CS ×(umol/L) concentration of 1,5-anhydroglucose alcohol in sample is calculated.
Mensuration obtained by the test kit of the table 1 mensuration 1,5-anhydroglucose alcohol obtained by embodiment 1 and embodiment 2
The result that quality-control product 1 is measured by the test kit of 1,5-AG respectively, wherein 1 in quality-control product 1,5-anhydroglucose
The concentration of alcohol is 120umol/L, and measurement result is shown in Table 1:
Table 1
As shown in Table 1, the measurement result deviation to quality-control product 1 of the test kit measuring 1,5-AG obtained by the present invention
Less, therefore accuracy of measurement is high, and embodiment 1 is optimum selection.
Mensuration 1,5-obtained by the test kit of the table 2 mensuration 1,5-anhydroglucose alcohol obtained by embodiment 1 and embodiment 2
The result that quality-control product 2 is measured by the test kit of anhydroglucose alcohol respectively, wherein 1,5-AG in quality-control product 2
Concentration is 280umol/L, and measurement result is shown in Table 2:
Table 2
| 1st time (umol/L) | 2nd time (umol/L) | 3rd time (umol/L) | Average (umol/L) | Deviation (%) | |
| Embodiment 1 | 280 | 284 | 282 | 282 | 0.71% |
| Embodiment 2 | 281 | 285 | 283 | 283 | 1.10% |
As shown in Table 2, the measurement result deviation to quality-control product 2 of the test kit measuring 1,5-AG obtained by the present invention
Less, therefore accuracy of measurement is high, and embodiment 1 is optimum selection.
Same sample to be tested is carried out many by the test kit of the table 3 mensuration 1,5-anhydroglucose alcohol obtained by embodiment 3
Secondary it is repeatedly measured and same sample to be tested is carried out many by the test kit of mensuration 1,5-anhydroglucose alcohol obtained by embodiment 4
Secondary being repeatedly measured, the result of gained carries out the calculating of SD and CV, result is as follows:
Table 3
The precision of the test kit measuring 1,5-AG obtained by the present invention is relatively good as shown in Table 3, and by table 1
Understanding, embodiment 3 is optimum selection.
The principle of above-described embodiment only illustrative present invention and effect thereof, not for limiting the present invention.Any ripe
Above-described embodiment all can be modified under the spirit and the scope of the present invention or change by the personage knowing this technology.Cause
This, have usually intellectual such as complete with institute under technological thought without departing from disclosed spirit in art
All equivalences become are modified or change, and must be contained by the claim of the present invention.
Claims (5)
1. the test kit measuring 1,5-AG, it is characterised in that: include reagent R1 independent of each other and reagent R2
Biliquid component, including composition and corresponding content be:
Reagent R1:
Tris buffer 20 ~ 180 mmol/L
Glucokinase 5 ~ 65 g/L
Pyruvate kinase 0.1 ~ 7.0 KU/L
Ascorbic acid oxidase 2.0 ~ 10.0 KU/L
Adenosine triphosphate 0.2 ~ 1.8 mmol/L
Phosphopyruvic acid enolate 1.5 ~ 6.5 mmol/L
4-amino antipyrine 0.2 ~ 1.8 mmol/L
MgCl2 5~25 mmol/L
KCl 10~150 mmol/L
Its solvent is purified water
Reagent R2:
Tris buffer 20 ~ 180 mmol/L
Pyranose oxidase 50 ~ 150 KU/L
Horseradish peroxidase 0.1 ~ 16 KU/L
3-hydroxyl-2,4,6-Triiodobenzoic acid 0.5 ~ 8.5 mmol/L
Its solvent is purified water.
A kind of test kit measuring 1,5-AG the most according to claim 1, it is characterised in that: include the most only
Vertical reagent R1 and reagent R2 biliquid component composition, including composition and corresponding content be:
Reagent R1:
Tris buffer 100 mmol/L
Glucokinase 35 g/L
Pyruvate kinase 3.5 KU/L
Ascorbic acid oxidase 6.0 KU/L
Adenosine triphosphate 1.0 mmol/L
Phosphopyruvic acid enolate 4.0 mmol/L
4-amino antipyrine 1.0 mmol/L
MgCl2 15 mmol/L
KCl 80 mmol/L
Its solvent is purified water
Reagent R2:
Tris buffer 100 mmol/L
Pyranose oxidase 100 KU/L
Horseradish peroxidase 8 KU/L
3-hydroxyl-2,4,6-Triiodobenzoic acid 4.5 mmol/L
Its solvent is purified water.
A kind of test kit measuring 1,5-AG the most according to claim 1 and 2, it is characterised in that: described
In reagent R1, the pH value of described Tris buffer is 6～9.
A kind of test kit measuring 1,5-AG the most according to claim 1 and 2, it is characterised in that: described
In reagent R2, the pH value of described Tris buffer is 6～9.
The preparation method of a kind of test kit measuring 1,5-anhydroglucose alcohol the most according to claim 1 and 2 and user
Method, it is characterised in that: comprise the following steps:
A () prepares reagent according to following component content:
Reagent R1:
Tris buffer 20 ~ 180 mmol/L
Glucokinase 5 ~ 65 g/L
Pyruvate kinase 0.1 ~ 7.0 KU/L
Ascorbic acid oxidase 2.0 ~ 10.0 KU/L
Adenosine triphosphate 0.2 ~ 1.8 mmol/L
Phosphopyruvic acid enolate 1.5 ~ 6.5 mmol/L
4-amino antipyrine 0.2 ~ 1.8 mmol/L
MgCl2 5~25 mmol/L
KCl 10~150 mmol/L
Its solvent is purified water
Reagent R2:
Tris buffer 20 ~ 180 mmol/L
Pyranose oxidase 50 ~ 150 KU/L
Horseradish peroxidase 0.1 ~ 16 KU/L
3-hydroxyl-2,4,6-Triiodobenzoic acid 0.5 ~ 8.5 mmol/L
Its solvent is purified water;
B test serum sample is mixed by () with reagent R1 and reagent R2 so that it is fully react；
C () measures reacted absorbance difference with automatic clinical chemistry analyzer；
D () calculates the concentration of 1,5-anhydroglucose alcohol in sample according to absorbance changing value.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610273306.3A CN106047988A (en) | 2016-04-28 | 2016-04-28 | A kit for measuring 1,5-anhydroglucitol and a preparing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610273306.3A CN106047988A (en) | 2016-04-28 | 2016-04-28 | A kit for measuring 1,5-anhydroglucitol and a preparing method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106047988A true CN106047988A (en) | 2016-10-26 |
Family
ID=57176172
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610273306.3A Pending CN106047988A (en) | 2016-04-28 | 2016-04-28 | A kit for measuring 1,5-anhydroglucitol and a preparing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106047988A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108007922A (en) * | 2017-12-21 | 2018-05-08 | 广州市进德生物科技有限公司 | A kind of kit using luminol chemiluminescence analysis detection glucose |
| CN110702676A (en) * | 2019-11-14 | 2020-01-17 | 北京华宇亿康生物工程技术有限公司 | Kit and method for detecting 1,5-AG with good stability |
| CN110846378A (en) * | 2019-11-01 | 2020-02-28 | 苏州普瑞斯生物科技有限公司 | 1, 5-sorbitan detection kit and preparation method thereof |
-
2016
- 2016-04-28 CN CN201610273306.3A patent/CN106047988A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| YUKIHITO FUKUMURA等: "Fully Enzymatic Method for Determining 1 ,5-Anhydro-D-glucitol in Serum", 《CLIN. CHEM.》 * |
| 张抗等: "血浆1 , 5 脱水葡糖醇在糖尿病监测中的应用和测定方法的进展", 《临床检验杂志》 * |
| 陈国军等: "血清1 ,5 脱水葡萄糖醇全酶的测定", 《中华医学检验杂志》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108007922A (en) * | 2017-12-21 | 2018-05-08 | 广州市进德生物科技有限公司 | A kind of kit using luminol chemiluminescence analysis detection glucose |
| CN108007922B (en) * | 2017-12-21 | 2018-11-13 | 广州市进德生物科技有限公司 | A kind of kit detecting glucose using luminol chemiluminescence analysis |
| CN110846378A (en) * | 2019-11-01 | 2020-02-28 | 苏州普瑞斯生物科技有限公司 | 1, 5-sorbitan detection kit and preparation method thereof |
| CN110702676A (en) * | 2019-11-14 | 2020-01-17 | 北京华宇亿康生物工程技术有限公司 | Kit and method for detecting 1,5-AG with good stability |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104198473B (en) | A kind of uric acid detection kit of stabilization | |
| CN104483487B (en) | Kit for detecting 1, 5-anhydro-D-ghlcitol in human blood | |
| CN102175670B (en) | Method for detecting 1,5-dehydration glucitol in blood and kit | |
| EP2319937B1 (en) | Blood component measurement method utilizing hemolyzed whole blood, and kit for the method | |
| CN107703071B (en) | Kit and method for detecting 1,5-AG | |
| CN106053368A (en) | Kit for determining microalbuminuria and preparation method thereof | |
| CN111394424B (en) | Human serum creatinine content detection reagent and method for resisting calcium dobesilate interference | |
| CN102154442B (en) | Method for detecting 1,5-anhydro sorbitol and related diagnostic kit | |
| CN112029817B (en) | Creatinine detection kit and application method thereof | |
| CN106047988A (en) | A kit for measuring 1,5-anhydroglucitol and a preparing method thereof | |
| CN101135688A (en) | Method for measuring fructosamine NBT in blood serum | |
| CN104237147B (en) | Determination of lactose method in body fluid | |
| CN106868096A (en) | A kind of stability is strong and hexokinase method glucose determination reagent of low cost | |
| CN107505470A (en) | Stable creatinine detection reagent box and its application method | |
| CN103837685B (en) | The detection method of a kind of blood glucose and detection kit | |
| CN106092924A (en) | A kind of test kit measuring free fatty and preparation method thereof | |
| CN101571485A (en) | Method and kit for measuring glucose | |
| CN108982829B (en) | A kind of method and its kit with lactulose in microplate reader enzyme process quantitative detection liquid milk | |
| CN108680569A (en) | Sucrose-determination method in serum | |
| CN102382875B (en) | High-concentration glucose removal method and kit for determining serum 1,5 anhydro-D-glucitol based on pyranose oxidase method | |
| CN103725749A (en) | Method for measuring 1,5-anhydroglucitol by oxidase | |
| CN108007922B (en) | A kind of kit detecting glucose using luminol chemiluminescence analysis | |
| CN107254508B (en) | H2O2Kit for detecting sialic acid by coupled indicator system | |
| CN106093386A (en) | A kind of test kit measuring creatine kinase isozyme (CK MB) | |
| CN103837487A (en) | Uric acid detection method and detection kit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161026 |
|
| RJ01 | Rejection of invention patent application after publication |