CN106037989B - Tissue repairing patch with fiber brush configuration and its preparation method and application - Google Patents
Tissue repairing patch with fiber brush configuration and its preparation method and application Download PDFInfo
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- CN106037989B CN106037989B CN201610353571.2A CN201610353571A CN106037989B CN 106037989 B CN106037989 B CN 106037989B CN 201610353571 A CN201610353571 A CN 201610353571A CN 106037989 B CN106037989 B CN 106037989B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/0059—Cosmetic or alloplastic implants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/0063—Implantable repair or support meshes, e.g. hernia meshes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/222—Gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/34—Materials or treatment for tissue regeneration for soft tissue reconstruction
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- Chemical & Material Sciences (AREA)
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- Chemical Kinetics & Catalysis (AREA)
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Abstract
The invention discloses a kind of tissue repairing patch with fiber brush configuration and preparation method thereof.The tissue repairing patch includes moulding body and electrospinning organization bracket, the electrospinning organization bracket be one or more electrospinning film roll made of center be nearly solid coil structure, or the sandwich construction built up for multiple electrospinning membrane stacks；The moulding body is made up of hydrophily degradation material, and the moulding body is coated on the surface of the electrospinning organization bracket and is filled between the electrospinning film for forming the electrospinning organization bracket；The fiber orientation directions of electrospinning film and the thickness direction of sticking patch are substantially in the same direction.The tissue repairing patch of the present invention, because the fiber-wall-element model of electrospinning film and the thickness direction of tissue repairing patch are identical, therefore cell is advantageous to along tissue thickness's direction growth, simultaneously, moulding body is made up of hydrophilic material, adhesion, the growth of cell can be promoted so that deeper defect obtains Rapid-Repair filling.
Description
Technical field
The present invention relates to the filling of tissue defect and tissue repair field, more particularly, to a kind of tissue repairing patch
And its preparation method and application.
Background technology
Electrospinning film has a wide range of applications in terms of tissue repair.Electrospinning film is commonly divided into orientation fiber（Also referred to as orient
Fiber）Electrospinning film and Random fiber two kinds of electrospinning film.Research finds, electrospinning film during tissue repair, cell
Migration is creeped substantially to be carried out along machine direction.Orientation process is carried out to electrospinning film, can inducing cell along fiber-wall-element model
Direction growth, obtain preferable repairing effect in the orientation direction.
The electrospinning film of existing orientation fiber, its fibrous differently- oriented directivity of institute is substantially along the extension direction of plane
Upper progress so that when electrospinning film is applied to surface of a wound surface, fiber extension direction growth of the cell along electrospinning film can be promoted.This
Electrospinning film of the kind with orientation fiber, defect face can be made by quick bridge joint repairing, but the repairing in thickness direction is but quite slow
Slowly.Therefore, the electrospinning film of existing orientation fiber, is more suitable for the shallower tissue repair of the surface of a wound.It is deeper for some defects
The surface of a wound, particularly such as the deeper chronic wound of diabetes, pressure sore defect, it is necessary to develop one kind can in a thickness direction also reality
The reparation product of existing Fast Filling repairing.
The content of the invention
The goal of the invention of the present invention is for overcome the deficiencies in the prior art, there is provided a kind of tissue with fiber brush configuration
Repair sticking patch.The tissue repairing patch can carry out Rapid-Repair to depth defect, for chronic depth defect also
It can reach and effectively eliminate tissue hydrops, reduce the effect that inflammation occurs.
Another goal of the invention of the present invention is the preparation method for providing the tissue repairing patch.
Another goal of the invention of the present invention is the application for providing the tissue repairing patch.
The above-mentioned purpose of the present invention is achieved by following technical solution：
A kind of tissue repairing patch with fiber brush configuration, the tissue repairing patch include moulding body and electrospinning tissue
Support, the electrospinning organization bracket be one or more electrospinning film roll made of center be nearly solid coil structure or be multiple
The sandwich construction that electrospinning membrane stack is built up；The moulding body is made up of hydrophily degradation material, and the moulding body is coated on described
The surface of electrospinning organization bracket and it is filled between the electrospinning film for forming the electrospinning organization bracket；The fiber of any electrospinning film
The thickness direction of differently- oriented directivity and sticking patch is substantially in the same direction.
When carrying out tissue repair using electrospinning film, the migration of cell is creeped substantially to be carried out along machine direction.By institute
The fiber orientation directions control of electrospinning organization bracket is stated into substantially in the same direction with thickness direction, cell can be promoted in a thickness direction
Fast-growth.However, depth defect portion is directly applied to after if directly existing directional fiber sticking patch is crimped/is superimposed
When position is repaired, not only the shape of product is without legal type, and due to curling or superposition after, the structure between layers of electrospinning film is very
Closely, therefore histocyte is difficult to grow into the electrospinning film of internal layer, makes repairing effect undesirable.The present invention passes through hydrophilic modeling
Body, assign fiber and repair the suitable structure of sticking patch, meanwhile, using in moulding body degradation process, cell liquid is slowly introduced into electricity
The inside of organization bracket is spun, improves repairing effect.
In the present invention, described coil structure can be, individual or multiple electrospinning films are crimped to one direction and formed
Cylinder-like structure, and make the cylindric center close to solid；Can also be of different sizes using multiple
Electrospinning film, be rolled into cylindric be set with into coil structure again respectively.
Tissue repairing patch of the present invention with fiber brush configuration, refer to the electrospinning group for forming tissue repairing patch
Support is knitted, its fiber orientation directions is identical with the thickness direction of tissue repairing patch so that tissue repairing patch has on microcosmic
Similar to the structure of brush.
Preferably, the moulding body has loose structure.When the moulding body has loose structure, it is more beneficial for carefully
Adhesion, the growth of born of the same parents, while its hydrophilicity is further lifted.
The combination of moulding body and electrospinning organization bracket, typically applies the solution for the material for forming moulding body to electricity
Spin on organization bracket, then the two combination is made by the method for solidification.
Preferably, the moulding body is combined with the electrospinning organization bracket by being freeze-dried mode.
Preferably, the average diameter of the fiber of the electrospinning film is 10nm ~ 100 μm.
The material for forming the electrospinning film can be non-degradable material either degradation material.When the electrospinning film by
When degradation material is formed, the degradation speed of the material will be slower than the degradation speed of moulding body material.I.e. when the electrospinning film by
When degradation material is formed, the degradation speed of the moulding body is faster than the degradation speed of the electrospinning film.
Preferably, form the electrospinning film material be selected from PLA, PGA, PEG, PCL, PVDF or PU any one or it is several
Kind.
Preferably, the hydrophily degradation material is selected from gelatin, collagen or polysaccharide.
Preferably, the moulding body is also present in forming in the internal void of the electrospinning film of the electrospinning organization bracket.
The preparation method of the tissue repairing patch, comprises the following steps：
S1. electrospinning fibre is made by electrostatic spinning in electrospinning membrane material, and carries out orientation process, obtaining has orientation fine
The electrospinning film of dimension；
S2. the solvent that can dissolve the hydrophily degradation material and insoluble electrospinning membrane material is chosen, by the parent
Uniform solution is made in water-based degradation material；
S3. center is made as nearly solid web-like supporting construction in one or more electrospinning film roll made of S1., be put into
S2. infiltrate in the uniform solution prepared, be then freeze-dried in the lump；Or by multiple electrospinning films made of S1. according to phase
Same fiber orientation directions are stacked, and are put into the uniform solution of S2. preparations and are infiltrated, are then freeze-dried in the lump；
S4. the material after S3. is freeze-dried takes out, and is cut, obtains the tissue repairing patch.
Preferably, in the S1, electrospinning film is prepared via a method which：Homogeneous certain density electrospinning membrane material is molten
Liquid is fitted into electrostatic spinning syringe, and the speed for adjusting micro-injection pump is 0.1 ~ 15.0 ml/hour, adjusts high pressure generator
Voltage be 5 ~ 45KV, the reception distance for adjusting reception device is 5.0 ~ 30.0 centimetres, regulation receive roller rotating speed be 300 turns/
More than minute, while orientation process is carried out, and fiber is received as membrane structure, stop electrostatic spinning after reaching certain thickness,
Obtain electrospinning film.
Preferably, the electrospun fiber membrane rolled or heap is folded is put into before the uniform solution of S2. preparations, first with alcohol pair
Electrospinning film is infiltrated.It can play a part of swelling because alcohol enters inside electrospinning film, therefore be advantageous to moulding liquid solution
Enter in the hole inside electrospinning film.
Preferably, in order to easy to operate, the electrospinning film rolled or heap is folded is put into the homogeneous solution in S3.
When, can make electrospinning film fiber orientation substantially vertically or the surface parallel to solution.
Preferably, in S3., lyophilized temperature is -20 DEG C~-100 DEG C.
Application of the tissue repairing patch in depth defect patching material is prepared.
Preferably, the depth defect is the diabetes as caused by diabetes, pressure sore or cartilage defect.
Compared with prior art, the present invention has the advantages that：
Tissue repairing patch of the present invention, the fiber orientation directions of its electrospinning organization bracket and the thickness of tissue repairing patch
It is identical to spend direction, in use, directly the sticking patch is implanted into defect（Correspond to the thickness of tissue according to the thickness direction of sticking patch
Spend direction implantation）, be advantageous to cell along tissue thickness's direction growth, meanwhile, moulding body is made up of hydrophilic material, can inhale
Tissue hydrops is received, and promotes the adhesion of cell, growth so that deeper defect obtains Rapid-Repair filling.Meanwhile tissue repair is mended
The overall structure of piece so that can prevent tissue fluid from building up during the entire process of tissue repair.
Brief description of the drawings
Fig. 1 is the structural representation of the tissue repairing patch of the present invention with fiber brush configuration.
Fig. 2 is the structural representation of the electrospinning organization bracket of tissue repairing patch prepared by embodiment 1.
Fig. 3 is another feasible configurations schematic diagram of electrospinning organization bracket.
Fig. 4 is another feasible configurations schematic diagram of electrospinning organization bracket.
Embodiment
The present invention is further explained with reference to embodiment, but specific embodiment is not to this hair
It is bright to be limited in any way.Unless stated otherwise, reagent involved in embodiment, method are reagent commonly used in the art and side
Method.
Embodiment 1
A kind of preparation method of the tissue repairing patch with fiber brush configuration, comprises the following steps：
S1. it is orientated the preparation of the electrospinning film of fiber
PLLA is dissolved in hexafluoroisopropanol solution, PLLA concentration is 8%（g/ml）, obtain uniform Polymer Solution；Will
PLLA solution is fitted into electrostatic spinning syringe, and the speed for adjusting micro-injection pump is 10 mls/hour, adjusts high pressure generator
Voltage be 22 KV, adjust reception device reception distance be 18 centimetres, adjust receiving roll rotating speed be 500 revs/min, enter
Row electrostatic spinning, electrostatic spinning is closed after reaching thickness 0.3mm, obtain PLLA electrostatic spinnings orientation tunica fibrosa.According to said method make
Standby multiple PLLA electrostatic spinnings orientation tunica fibrosa.
S2. the preparation of moulding liquid solution
Gelatin is dissolved in 50 DEG C of aqueous solution for injection, the concentration of gelatin is 3%（g/ml）, it is molten to obtain uniform gelatin
Liquid.
S3. freeze
The electrospinning film of orientation fiber in step S1. is close to curling in rolls along perpendicular to the direction of fiber-wall-element model, with one
Or multiple tunica fibrosas be rolled into an a diameter of 5cm cylindric supporter.After first infiltrating 5min with 75% alcohol transition, then stand
Put and complete wetting is in 50 DEG C of gelatin solutions prepared by step S2., infiltrate 2 hours.Then it is put into freeze-drying together
In case, -80 DEG C lyophilized 24 hours.
S4. cut
Sample after will be lyophilized in step S3., according to defect thickness, along perpendicular to the section of fiber orientation directions
Cut out the diaphragm of respective thickness, the as tissue repairing patch with fiber brush configuration.
S5. the tissue repairing patch with fiber brush configuration step S4. obtained is cleaned, and is packed, sterilizing.
The structure manufactured in the present embodiment for repairing sticking patch is as depicted in figs. 1 and 2.Fig. 1 is the overall structure for repairing sticking patch, its
In, 1 is the structure of electrospinning organization bracket, and 2 be moulding body；Fig. 2 is the structural representation of Fig. 1 electrospinning organization bracket, and it is by one
Center made of electrospinning film roll is opened for nearly solid web-like supporting construction, the direction of the fiber-wall-element model of the electrospinning film and electrospinning film
Roll direction as shown in Fig. 2 the fiber orientation directions of the electrospinning film of remaining accompanying drawing are identical with Fig. 2.Fig. 3 is electrospinning organization bracket
Another structure, by the different electrospinning film of multiple sizes from small to large successively socket form concentric web-like supporting construction.
Embodiment 2
A kind of preparation method of the tissue repairing patch with fiber brush configuration, comprises the following steps：
S1. it is orientated the preparation of fiber electrospinning film
PVDF is dissolved in the mixed solution of acetone and N,N-dimethylformamide（Acetone and N,N-dimethylformamide
Volume ratio is 3:2）, PVDF concentration is 18%（g/ml）, obtain uniform Polymer Solution；PVDF solution is loaded into Static Spinning
In silk syringe, the speed for adjusting micro-injection pump is 8 mls/hour, and the voltage for adjusting high pressure generator is 30 KV, regulation
The reception distance of reception device is 20 centimetres, and the rotating speed for adjusting receiving roll is 500 revs/min, carries out electrostatic spinning, reaches thickness
Electrostatic spinning is closed after 0.3mm, obtains PVDF electrostatic spinnings orientation tunica fibrosa.Multiple PVDF electrostatic spinnings are according to said method prepared to take
To tunica fibrosa.
S2. the preparation of moulding liquid solution
Gelatin is dissolved in 50 DEG C of aqueous solution for injection, the concentration of gelatin is 3%（g/ml）, it is molten to obtain uniform gelatin
Liquid.
S3. freeze
Multiple electrospinning films with fiber-wall-element model in step S1. are stacked into thickness according to identical fiber orientation directions
Folded for 5cm tunica fibrosa, with 75% alcohol by its pre-invasion, and according to tunica fibrosa differently- oriented directivity parallel to moulding liquid solution
Liquid level complete wetting infiltrates 2 hours in 50 DEG C of gelatin solutions prepared by step S2..Then it is put into freeze-drying together
In case, -80 DEG C lyophilized 24 hours.
S4. cut
Sample after will be lyophilized in step S3., according to defect thickness, along perpendicular to the section of fiber orientation directions
Cut out the diaphragm of respective thickness, the as tissue repairing patch with fiber brush configuration.
S5. the tissue repairing patch with fiber brush configuration step S4. obtained is cleaned, and is packed, sterilizing.
The electrospinning organization bracket structure manufactured in the present embodiment for repairing sticking patch is as shown in figure 4, by multiple electrospinning membrane stack shapes
Into.
Application Example
Selection miniature pig 6,12 ~ 15kg of body weight, preoperative 12h taboos diet, preoperative 30min intramuscular injection 0.5g atropines,
And with ketamine（5mg/Kg）, Su Mian Xin, 3% yellow Jackets（30mg/Kg）Carry out intramuscular injection mixed anesthesia.In every pig
Back shaving, and with iodophor disinfection skin twice.4 holes are made a call to the back backbone both sides of pig are each with diameter 5cm card punch
And deep and deep fascia shallow-layer, i.e. every pig produce 8 surface of a wound, totally 48 surface of a wound.
With blank group (A) and common electrospinning film group (B) for control group, with the tissue repairing patch group of embodiment 1（C）And reality
Apply the tissue repairing patch group of example 2（D）Tested for experimental group, it is specific as follows：
Blank group（A）：Every pig chooses 2 surface of a wound, antiseptic gauze is directly covered on the surface of a wound, and pricked and added with suture anti-package
Pressure, outer layer covering yarn pad, the pressurization of outermost layer elastoplast are fixed.Compression wrap, route dressing change, antiseptic gauze covering are removed after 2 weeks
The surface of a wound.
Common electrospinning film group（B）：Every pig chooses 2 surface of a wound, and common PLLA electrospinning films are filled on the surface of a wound（Multi-laminate
Add）, antiseptic gauze is then covered, and pricked and pressurizeed with suture anti-package, outer layer covering yarn pad, outermost layer elastoplast pressurization is fixed.2
Compression wrap, route dressing change, antiseptic gauze flap coverage are removed after week.
The tissue repairing patch group of embodiment 1（C）：Every pig chooses 2 surface of a wound, fills on the surface of a wound and is made in embodiment 1
The standby tissue repairing patch with fiber brush configuration, antiseptic gauze is then covered, and pricked and pressurizeed with suture anti-package, outer layer covering
Yarn pad, the pressurization of outermost layer elastoplast are fixed.Compression wrap, route dressing change, antiseptic gauze flap coverage are removed after 2 weeks.
The tissue repairing patch group of embodiment 2（D）：The implantation material of selection has fiber brush for what is prepared in embodiment 2
The tissue repairing patch of structure, remaining tissue repairing patch group with embodiment 1（C）It is identical.
Experimental result：
Observed after performing the operation 2 weeks, blank group（A）The surface of a wound have fresh granulation tissue growth, common electrospinning film group（B）Material table
Still for white and there is part sepage outflow in face, the tissue repairing patch group with fiber brush configuration（C and D）The smooth clean nothing of the surface of a wound
Sepage.Month after operation, blank group（A）Wound Contraction is substantially healed in a manner of scar.Common electrospinning film group（B）The surface of a wound, which still has, to be oozed
Liquid stream goes out, several layers of above PLLA films to take off easily, and lower two layers are mutually connected into pale red with tissue, be not easy to take off from.With fiber
The tissue repairing patch group of brush configuration（C and D）The clean smooth no sepage of the surface of a wound, is firmly combined with surrounding tissue, and the surface of a wound is into pink
Color.Illustrate the tissue repairing patch with fiber brush configuration of the invention to depth defect with good repairing effect.
Obviously, the above embodiment of the present invention is only intended to clearly illustrate example of the present invention, and is not pair
The restriction of embodiments of the present invention.For those of ordinary skill in the field, may be used also on the basis of the above description
To make other changes in different forms.There is no necessity and possibility to exhaust all the enbodiments.It is all this
All any modification, equivalent and improvement made within the spirit and principle of invention etc., should be included in the claims in the present invention
Protection domain within.
Claims (11)
1. a kind of tissue repairing patch with fiber brush configuration, it is characterised in that the tissue repairing patch includes moulding body
With electrospinning organization bracket, the electrospinning organization bracket be one or more electrospinning film roll made of center be nearly solid web-like knot
Structure or the sandwich construction built up for multiple electrospinning membrane stacks；The moulding body is made up of hydrophily degradation material, the moulding body
It is coated on the surface of the electrospinning organization bracket and is filled between the electrospinning film for forming the electrospinning organization bracket；Any electricity
The fiber orientation directions of spinning film and the thickness direction of sticking patch are substantially in the same direction.
2. tissue repairing patch according to claim 1, it is characterised in that the moulding body has loose structure.
3. tissue repairing patch according to claim 1, it is characterised in that the moulding body leads to the electrospinning organization bracket
Freeze-drying mode is crossed to combine.
4. tissue repairing patch according to claim 1, it is characterised in that the electrospinning film is by non-degradable material or can drop
Solve material to form, when the electrospinning film is made up of degradation material, the degradation speed of the moulding body is faster than the electrospinning film
Degradation speed.
5. tissue repairing patch according to claim 1, it is characterised in that form the electrospinning film material be selected from PLA,
PGA, PEG, PCL, PVDF or PU any one or a few.
6. tissue repairing patch according to claim 1, it is characterised in that the hydrophily degradation material be selected from gelatin,
Collagen or polysaccharide.
7. the preparation method of any one of claim 1 to 6 tissue repairing patch, it is characterised in that comprise the following steps：
S1. electrospinning fibre is made by electrostatic spinning in electrospinning membrane material, and carries out orientation process, obtained with orientation fiber
Electrospinning film；
S2. the solvent that can dissolve the hydrophily degradation material and insoluble electrospinning membrane material is chosen, by the hydrophily
Uniform solution is made in degradation material；
S3. center is made as nearly solid coil structure in one or more electrospinning film roll made of S1., is put into S2. preparations
Infiltrate in uniform solution, be then freeze-dried in the lump；Or multiple electrospinning films made of S1. are taken according to identical fiber
Stacked to direction, be put into the uniform solution of S2. preparations and infiltrate, be then freeze-dried in the lump；
S4. the material after S3. is freeze-dried takes out, and is cut, obtains the tissue repairing patch.
8. the preparation method of tissue repairing patch according to claim 7, it is characterised in that the temperature of lyophilization in S3.
Spend for -20 DEG C~-100 DEG C.
9. the preparation method of tissue repairing patch according to claim 7, it is characterised in that be put into electrospinning film in S3.
S2. before the uniform solution prepared, first electrospinning film is infiltrated with alcohol.
10. application of any one of claim 1 to 6 tissue repairing patch in depth defect patching material is prepared.
11. application according to claim 10, it is characterised in that the depth defect refers to diabetes, pressure sore or soft
Cranial defect.
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| CN104721886A (en) * | 2015-02-12 | 2015-06-24 | 广州迈普再生医学科技有限公司 | Tissue repair membrane with fiber structure, preparation method, using method and application thereof |
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Address after: 510663 Guangdong Guangzhou high tech Industrial Development Zone, Science City, 80 E third district. Patentee after: Guangzhou Maple regenerative medicine Polytron Technologies Inc Address before: 510663 Guangzhou District, Guangdong, new high tech Industrial Development Zone, Science City, 80 E third district. Patentee before: Medprin Regenerative Medical Technologies Co., Ltd. |