CN106035386A - Nematicidal composition containing paecilomyces lilacinus and fosthiazate - Google Patents

Nematicidal composition containing paecilomyces lilacinus and fosthiazate Download PDF

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Publication number
CN106035386A
CN106035386A CN201610613612.7A CN201610613612A CN106035386A CN 106035386 A CN106035386 A CN 106035386A CN 201610613612 A CN201610613612 A CN 201610613612A CN 106035386 A CN106035386 A CN 106035386A
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CN
China
Prior art keywords
fosthiazate
paecilomyces lilacinus
root
nematicidal composition
knot nematode
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Pending
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CN201610613612.7A
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Chinese (zh)
Inventor
叶宇平
杨建江
耿鹏
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Foshan Yinghui Crop Science Co Ltd
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Foshan Yinghui Crop Science Co Ltd
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Priority to CN201610613612.7A priority Critical patent/CN106035386A/en
Publication of CN106035386A publication Critical patent/CN106035386A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/26Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-nitrogen bonds
    • A01N57/32Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-nitrogen bonds containing heterocyclic radicals

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to a nematicidal composition containing paecilomyces lilacinus and fosthiazate. The composition contains paecilomyces lilacinus, wherein the weight ratio of the paecilomyces lilacinus to the fosthiazate is 1:80 to 100:1. Two active ingredients of the composition disclosed by the invention take a synergistic effect mutually and can improve prevention and treatment effects mutually, the use quantity of pesticides is reduced, and the nematicidal composition is safe for the environment and is valuable to popularize and apply.

Description

A kind of nematicidal composition comprising Paecilomyces lilacinus and fosthiazate
Technical field
The invention belongs to field of pesticide preparation, particularly relate to a kind of composition pesticide preventing and treating agricultural root-knot nematode.
Background technology
Root-knot nematode is mainly caused harm the root of various vegetable, shows as side root and fibrous root compared with normal increases, and at young root Form the white root nodule that spherical or cone differs in size on fibrous root, have in beads shape.The growth of killed strain overground part is short and small, slow Slowly, leaf color anomaly, result is few, yields poorly, and even causes plant ahead of time death.The nematicide being currently known vegetable of causing harm mainly has height Bow root-knot nematode, peanut root-knot nematode, M hapla and Meloidogyne incognita.Nematicide host range is extensive, melon of often causing harm The various vegetables such as class, solanaceous vegetables, beans and Radix Raphani, Caulis et Folium Lactucae sativae, Chinese cabbage, moreover it is possible to propagate some funguses and bacterial disease.
Fosthiazate (fosthiazate) is organic phosphates nematicide, and main effect model is suppression root-knot nematode acetyl The synthesis of acetylcholine esterase, can be used for preventing and treating all kinds of root-knot nematode, aphid etc..In China achieved with on Fructus Cucumidis sativi, Fructus Lycopersici esculenti, Citrullus vulgaris Registration, can be widely applied to the crops such as vegetable, Fructus Musae, fruit tree, medical material.
Paecilomyces lilacinus, is effective bacterial parasite of Meloidogyne incognita and white cyst roundworm ovum, to Meloidogyne incognita Percentage of egg parasitism be up to 60-70%.Multiple nematicide there is is prevention effect, is the preventing and treating the most promising biological prevention and control agent of root-knot nematode. This fungi preparation by this bacterium commercialization, is sold with BIOCON in technique center, Philippine Asia.At home, Hua Yuanfeng agriculture is biological Science and Technology Ltd. releases the bottled preparation of 100g/ bottle with a 10000000000/gram spore preparation alive, has listed.Paecilomyces lilacinus secondary generation Thank to the physiological function of thing: root system of plant executes bacterium can not only substantially suppress nematode infection, and can promote the life of vegetable nutritorium Long, sprouting and growth to seed simultaneously also has facilitation;Can produce abundant chitinase, chitin is had by chitinase Explanation effect, it can promote the hatching of line eggs, improve the parasitic rate that line is filled by paecilomycerol;The molten of difficultly-soluble phosphates can be promoted Solving, laboratory research confirms: the solubilizing effect of Paecilomyces varioti reaches 30%, and nematodes Antagonistic Fungi reaches 20-40%.Research simultaneously is also Showing, Paecilomyces varioti can also promote the decomposition of many chemical polymerization things (such as pesticide, leather-making waste water etc.), and this proves Paecilomyces lilacinus tool There is certain environmental protection effect.
Agricultural production business is due to use long-term, single or pesticide abuse, and insect creates Drug resistance to insecticide, very To developing into cross tolerance or multiresistance so that many pesticide species lose effectiveness.And the development cycle of novel pesticide is long, past Toward not giving material assistance to again, this is that agricultural protects the major obstacles produced.The progress of pesticide research shows: will overcome and delay the drug resistance of insect Property, extend the service life of old pesticide species, carry out agriculture chemical compounding use, be an effective measure.
Summary of the invention
Present invention aims to the deficiencies in the prior art, it is provided that a kind of have preferable prevention effect to root-knot nematode The nematicidal composition comprising Paecilomyces lilacinus and fosthiazate, the weight ratio of described Paecilomyces lilacinus and fosthiazate is 1:80- 100:1, wherein, Paecilomyces lilacinus is 1-100 hundred million CFU/g containing viable bacteria amount, preferably 500,000,000 CUF/g.
Further, the weight ratio of described Paecilomyces lilacinus and fosthiazate is 1:40-40:1.
Further, described nematicidal compositions also includes the agricultural active composition of other kind.
The nematicidal composition of the present invention can by conventional formulation preparation method be prepared as in agricultural production any A kind of dosage form, described dosage form can be wettable powder, granule, suspending agent, powder etc..
Further, containing conventional auxiliary element in described preparation, described auxiliary element can be stabilizer, molten Agent and other auxiliary agent;Wherein, auxiliary agent can select attapulgite, Kaolin, meerschaum, precipitated calcium carbonate, diatomaceous one Or multiple combination;Stabilizer can be selected from Oleum Ricini, soybean oil, epoxy soybean oil, propylene glycol, methyl oleate, ethylene glycol, BHT, One or more compositionss in BHA;Solvent can select the one in toluene, dimethylbenzene, trimethylbenzene, dimethylformamide or Multiple.
Having the beneficial effect that of the nematicidal composition of the present invention
(1) nematicidal composition of the present invention, the preventing and treating to nematicide serves synergistic function each other, decreases The usage amount of pesticide, adds prevention effect, is conducive to saving drug cost and the pollution to environment;
(2) nematicidal composition of the present invention has that technique is easy to control, the advantage of constant product quality, it is possible to produce continuously, suitable Together in industrialization.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, present invention specific examples below Illustrate, but the present invention is limited to absolutely not these examples.The following stated is only the preferable embodiment of the present invention, is used only for explaining The present invention, but can not be therefore understands that be the restriction to the scope of the claims of the present invention.Should be understood that time, all spirit in the present invention and Any amendment, equivalent or the improvement etc. made within principle, should be included within the scope of the present invention, therefore, The protection domain of patent of the present invention should be as the criterion with claims.
Test active component: Paecilomyces lilacinus is 500,000,000 CFU/g Paecilomyces lilacinus granules, and fosthiazate effective ingredient contains Amount is 93%, and above-mentioned raw materials is provided by technology department of Ying Hui crop science company limited.
Specific embodiment: Paecilomyces lilacinus and the fosthiazate preventive effect to Fructus Cucumidis sativi root-knot nematode
Application Paecilomyces lilacinus and fosthiazate process Fructus Cucumidis sativi root-knot nematode, including be used alone preventing and treating root-knot nematode and two kinds have Effect composition is mixed uses preventing and treating root-knot nematode, it is provided that the granule that mixed scheme is Paecilomyces lilacinus and fosthiazate by following Method prepares: fosthiazate is dissolved in the homogenizer equipped with solvent and cosolvent by (1);(2) by binding agent and stable Agent, Paecilomyces lilacinus are mixed after being equipped with in the homogenizer of above-mentioned solution and are homogenized, and obtaining outward appearance has certain viscosity, all The fosthiazate of one and Paecilomyces lilacinus admixing medical solutions;(3) prepare fosthiazate admixing medical solutions transfer to can be quantitative high-level container In;(4) carrier quantitation manufacturing granule is rendered in agitator;(5) start agitator, open the tapping of high-level container Valve, is quantitatively adding the fosthiazate mixed and Paecilomyces lilacinus admixing medical solutions, until medicinal liquid adds;(6) check that absorption method is made The effect of granule, and all technical of inspection product.The present invention compared with prior art, has equipment investment few, technique Easy to control, constant product quality, it is possible to produce continuously, it is suitable for the feature of industrialized production.
The Paecilomyces lilacinus of different ratio of the present invention and the nematicidal composition of fosthiazate is used to be used for preventing and treating Fructus Cucumidis sativi root knot Nematicide field control effectiveness test, often processes in triplicate, the repetition of one, every community, uses and goes at random to rent arrangement, plot area 20 m2.Shallow mixed in broadcasting preplanting treatment dispenser, sow at random.30 days investigation root-knot nematode quantity after medicine.Employing diagonal takes Sample method, every some sampling 3 strains, investigation disease index and the Recording criteria of sickness rate root-knot nematode harm, raw many according to root knot Major general's state of an illness is divided into Pyatyi:
0 grade without root knot;
1 grade of root knot accounts for the 1-25% of full root system;
2 grades of root knots account for the 26-50% of full root system;
3 grades of root knots account for the 51-75% of full root system
4 grades of root knots account for the 76-100% of full root system
According to formula (5) and the prevention effect of formula (6) calculating Fructus Cucumidis sativi root-knot nematode:
The value of preventive effect refers to that the percentage rate killing nematicide represents, calculates the potentiation situation of two kinds of medicaments according to colby formula, Result of the test is as shown in table 1.
Field control effectiveness test result shows, after Paecilomyces lilacinus and fosthiazate mixing, the preventing and treating to Fructus Cucumidis sativi root-knot nematode exists Show potentiation in the range of certain proportion, in the range of 1:80-100:1, all show as potentiation.Meanwhile, in test During, after compounding to Paecilomyces lilacinus and fosthiazate, it being applied to Fructus Cucumidis sativi, the growing way of Fructus Cucumidis sativi is more preferable, in scope of medication, does not sends out The compositions of the existing present invention produces poisoning to Fructus Cucumidis sativi, shows that the compositions safety of the present invention is also preferable, is worth on producing Popularization and application.

Claims (4)

1. a composition pesticide, it is characterised in that active component comprises Paecilomyces lilacinus and fosthiazate.
Composition pesticide the most according to claim 1, it is characterised in that described Paecilomyces lilacinus and the weight ratio of fosthiazate For 1:80-100:1.
3. according to the composition pesticide of claim 1 or 2, it is characterised in that the dosage form of compositions is wettable powder, granule In agent, suspending agent, powder any one.
4. according to the purposes of the composition pesticide described in claim 1-3 any one claim, it is characterised in that be used for preventing and treating Root-knot nematode.
CN201610613612.7A 2016-08-01 2016-08-01 Nematicidal composition containing paecilomyces lilacinus and fosthiazate Pending CN106035386A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106942280A (en) * 2017-03-23 2017-07-14 佛山市普尔玛农化有限公司 Nematicidal composition containing tioxazafen and Paecilomyces lilacinus
CN108294050A (en) * 2018-03-16 2018-07-20 佛山市普尔玛农化有限公司 Including Paecilomyces lilacinus kills line composition

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102657155A (en) * 2012-05-10 2012-09-12 肇庆市真格生物科技有限公司 Nematicide granules with sawdust as carrier and preparation method thereof
US20150296803A1 (en) * 2012-12-03 2015-10-22 Bayer Cropscience Ag Composition comprising a biological control agent and an insecticide
CN105112070A (en) * 2015-09-15 2015-12-02 北京好收成全农生物科技有限公司 Acid soil conditioner and preparation method thereof
CN105660711A (en) * 2016-04-01 2016-06-15 广西南宁益土生物科技有限责任公司 Nematode granules with seedling recovering effect

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102657155A (en) * 2012-05-10 2012-09-12 肇庆市真格生物科技有限公司 Nematicide granules with sawdust as carrier and preparation method thereof
US20150296803A1 (en) * 2012-12-03 2015-10-22 Bayer Cropscience Ag Composition comprising a biological control agent and an insecticide
CN105112070A (en) * 2015-09-15 2015-12-02 北京好收成全农生物科技有限公司 Acid soil conditioner and preparation method thereof
CN105660711A (en) * 2016-04-01 2016-06-15 广西南宁益土生物科技有限责任公司 Nematode granules with seedling recovering effect

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106942280A (en) * 2017-03-23 2017-07-14 佛山市普尔玛农化有限公司 Nematicidal composition containing tioxazafen and Paecilomyces lilacinus
CN108294050A (en) * 2018-03-16 2018-07-20 佛山市普尔玛农化有限公司 Including Paecilomyces lilacinus kills line composition

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