CN106035215B - Blackspot side pleat frog cultural method - Google Patents
Blackspot side pleat frog cultural method Download PDFInfo
- Publication number
- CN106035215B CN106035215B CN201610424927.7A CN201610424927A CN106035215B CN 106035215 B CN106035215 B CN 106035215B CN 201610424927 A CN201610424927 A CN 201610424927A CN 106035215 B CN106035215 B CN 106035215B
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- CN
- China
- Prior art keywords
- blackspot
- tadpole
- frog
- cylinder mould
- pleat frog
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
Abstract
A kind of blackspot side pleat frog cultural method, in abnormal successfully breeding process, the natural enemy of tadpole is put into the pleat frog spawning of blackspot side to tadpole in culturing pool, and natural enemy is closed in cylinder mould, and cylinder mould is placed in water.So the ovum of the blackspot side pleat frog on the Odor stimulation periphery of natural enemy and tadpole, not only facilitate the resistance against diseases of enhancing tadpole, and increase tadpole is abnormal for the body weight and body of blackspot side pleat froglet are long, so as to improve the benefit and yield of blackspot side pleat frog cultivation.
Description
Technical field
The present invention relates to frog culture technical field, more particularly to a kind of blackspot side pleat frog cultural method.
Background technology
At present, in the breeding process of the blackspot side pleat frog, raiser in order to improve income, produce by the raising that will try every possible means
Amount, therefore cultivation must first clear up culturing pool, and natural enemy and the rival of the blackspot side pleat frog and tadpole are killed, it is exactly in fact to kill
Other biological in dead culturing pool, there is provided the environment of single cultivation is carried out to the blackspot side pleat frog.Under such environment, the blackspot side pleat frog
Lack some viabilities in developmental process, be unfavorable for growing up healthy and sound for the blackspot side pleat frog, thus it is liable to illness or weak, it is individual
Long is little.And quickly increase the blackspot side pleat frog generally by increase feed and nourishing additive agent in the prior art, this
Sample not only increases cost, and is unfavorable for the meat of the blackspot side pleat frog.
The content of the invention
The invention solves the problems that the problem that above-mentioned prior art is present, there is provided a kind of blackspot side pleat frog cultural method, by day
Enemy stimulates the growth of blackspot side pleat frog tadpole, not only increases the volume after the pleat frog metamorphosis of blackspot side, and raising offspring adapts to energy
Power and meat.
The present invention solves the technical scheme that its technical problem is used：This blackspot side pleat frog cultural method, in blackspot side pleat
The frog is laid eggs in the abnormal successfully breeding process of tadpole, and the natural enemy of tadpole is put into culturing pool, and natural enemy is closed in cylinder mould, cylinder mould
It is placed in water.The so ovum of the Odor stimulation periphery blackspot side pleat frog of natural enemy and tadpole, can not only strengthen tadpole existence and
Premunition, after tadpole is abnormal for blackspot side pleat froglet, the volume also relative increase of blackspot side pleat froglet, body weight becomes weight, so that
Improve the adaptive capacity to environment and yield of the blackspot side pleat frog.
In order to further perfect, the preferred cray of natural enemy or mosquito fish.Cray or mosquito fish are the ovum of the blackspot side pleat frog
With the natural enemy of tadpole, the effect of stimulation produced in water is put into preferable.
Further perfect, cylinder mould has several and is spaced 3-5 ㎡ puts a cylinder mould, per 15 crays of cylinder mould.Due to day
The smell of enemy is diffused with certain limit, therefore interval 3-5 ㎡ put a cylinder mould, is distributed smell gamut.
It is further perfect, cylinder mould daytime 6-9 points and 18-21 points be put into culturing pool water in.The blackspot side pleat frog is in early, evening
Activity is more, therefore the stimulation with two time period increases of evening to it, the health of the enhancing blackspot side pleat frog in the morning, is conducive to
Put on weight.
It is further perfect, after the pleat frog spawning of blackspot side, stimulation liquid is poured into culturing pool, comprise the following steps that：
The first step, preparing stimulates liquid：50 crays in 50 blackspot side pleat frog tadpoles and cylinder mould are put into container,
After 12h, blackspot side pleat frog tadpole and cray are pulled out, solution is obtained into clear filtrate with filtered through gauze, that is, stimulate liquid；
Second step, liquid is stimulated to being added in culturing pool, and interval 12h is added and once stimulated liquid.
The distribution of cylinder mould is difficult visibly homogeneous, and tadpole away from the cylinder mould equipped with natural enemy, therefore using liquid is stimulated, can stimulate liquid
The intensive region of tadpole is can be sprayed on, stimulates the effect of liquid substantially, according to the appropriate sprinkling of tadpole distribution.
The invention has the advantages that：The present invention stimulates ovum and the tadpole of the blackspot side pleat frog using unique method, not only
Strengthen the premunition of tadpole, increase the head of tadpole, after tadpole is abnormal for the blackspot side pleat frog, the volume of the blackspot side pleat frog also phase
To increase, body weight becomes weight, so as to improve the yield of the blackspot side pleat frog, is conducive to being widely popularized.
Brief description of the drawings
Fig. 1 is the abnormal time of froglet, NP：Without natural enemy, EP：There is natural enemy；
Fig. 2 is long for the body of froglet, NP：Without natural enemy, EP：There is natural enemy；
Fig. 3 is the body weight of froglet, NP：Without natural enemy, EP：There is natural enemy；
Fig. 4 is the skip capability of froglet, NP：Without natural enemy, EP：There is natural enemy.
Specific embodiment
With reference to embodiment, the invention will be further described：
1 materials and methods
1.1 experiment equipments
Phjytotron (10M2), it is assay balance (± 0.0001g, Sai Duolisi scientific instrument Co., Ltd), biochemical permanent
Warm incubator, XTS3 types anatomical lens (Beijing Tyke Instrument Ltd.), plant pulverizer (the Tianjin limited public affairs of Stettlen instrument
Department), digital camera (Sony DSC-T100), electronic digital indicator (± 0.01mm), HOBO record blocks and software, plastic box
(700L×500W×400H mm3), plastic bowl (bore 20mm), dredger, culture dish, Morris type water mazes video tracking point
Analysis system (Anhui Zhenghua Biology Instrument Equipment Co., Ltd.) etc..
1.2 experimental animals gather and spawn incubation
At night, irradiate big with nearly 15 of dredger (mesh 2mm) manual capture from persistence culturing pool by flashlight
Small similar cray, takes back amphibians faunal laboratory, raises in outdoor tank-culture (3.0 × 2.0 × 1.5m3) in it is standby
With starvation 24h before experiment.
Number nest blackspot side pleat frog's egg group is gathered from identical persistence culturing pool, amphibians faunal laboratory is taken back,
It is momentarily placed in the square big plastic box (700L × 500W × 400H mm in climate room (20.8 ± 0.2 DEG C)3, the 200mm depth of waters) and it is standby
With.
1.3 experimentations
200 pieces are taken out at random from above-mentioned this nest blackspot side pleat frog embryonated egg, is divided into 2 parts, be sequentially placed into square plastic hopper
(180×130×60mm3, the depth of water is 6cm) in, numbering is 1 and 2, then carries out following 2 conditions treatment respectively：It is small without predator
The blank of lobster, it is put into cray and processes always.
There are two forelimbs (42 phase) using tadpole and indicate two forelimb afterbodys occur to tadpole since experiment as metamorphosis
It is wholly absent experienced number of days and is designated as period of metamorphosis.Body weight is weighed, and froglet body is determined with electronic digital indicator (± 0.01mm)
The distance between long (from kiss end to urostyle).
Every blackspot side pleat froglet after (25 ± 1 DEG C) endoadaptation 10-15min, puts it into 1% non-polluted edible indoors
Its four limbs is soaked in pigment solution, is placed in after pulling out and is covered with the ground of white antiskid paper, its tail is gently touched with willow branch
Bar bone stimulates it to jump, and at least allows every froglet to jump more than for 10 times.Jump length is determined by coating vestige, from upper one
The end of individual hind leg trace is a jump to the distance between the end of next hind leg trace, is measured with ruler most long by three
Secondary skip distance (± 0.01cm), obtains average value.
2 results
Fig. 1 is shown in the statistical description of abnormal correlated characteristic.Single factor test multivariate analysis of variance (One-way MANOVA) is tied
Fruit shows, different predators set abnormal time of tadpoles, body long and body weight, skip distance significant difference (Wilks λ=0.83,
Df=4,88, P<0.002), Tukey ' s test show that the body of froglet is long and body weight is notable under conditions of there is no predator
Less than the situation (allP that predator is present<0.004), the not notable (P of two kinds of abnormal times for setting and froglet skip distance difference
>0.10)。
, it is apparent that different letters represents significant difference (a from the icon of Fig. 1,2,3 and 4>B), NP：Without day
Enemy, EP：There is natural enemy, in the environment of having a natural enemy, abnormal time of the blackspot side pleat frog, body long, body weight and skip capability have increase.
Although the present invention is described by reference to preferred embodiment, those skilled in the art should
Work as understanding, in the range of claims, the various change in form and details can be made.
Claims (3)
1. a kind of blackspot side pleat frog cultural method, it is characterized in that：In blackspot side, the spawning of the pleat frog was successfully cultivated to tadpole metamorphosis
Cheng Zhong, is put into the natural enemy of tadpole in culturing pool, and natural enemy is closed in cylinder mould, and cylinder mould is placed in water；The natural enemy be cray or
Mosquito fish；
After the pleat frog spawning of blackspot side, stimulation liquid is poured into culturing pool, comprised the following steps that：
The first step, preparing stimulates liquid：50 crays in 50 blackspot side pleat frog tadpoles and cylinder mould, 12h are put into container
Afterwards, blackspot side pleat frog tadpole and cray are pulled out, solution is obtained into clear filtrate with filtered through gauze, that is, stimulate liquid；
Second step, liquid is stimulated to being added in culturing pool, and being added sooner or later at interval of 12h once stimulates liquid.
2. pleat frog cultural method in blackspot side according to claim 1, it is characterized in that：The cylinder mould puts a net per 3-5 ㎡
Cage, per 15 crays of cylinder mould.
3. pleat frog cultural method in blackspot side according to claim 1, it is characterized in that：The cylinder mould is in 6-9 points and 18- on daytime
21 points are put into the water of culturing pool.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610424927.7A CN106035215B (en) | 2016-06-16 | 2016-06-16 | Blackspot side pleat frog cultural method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610424927.7A CN106035215B (en) | 2016-06-16 | 2016-06-16 | Blackspot side pleat frog cultural method |
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| Publication Number | Publication Date |
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| CN106035215A CN106035215A (en) | 2016-10-26 |
| CN106035215B true CN106035215B (en) | 2017-06-23 |
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Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0974694B1 (en) * | 1997-03-25 | 2004-06-02 | Chelest Corporation | Chelate-forming fiber, process for preparing the same, and use thereof |
| CN101543193B (en) * | 2008-03-28 | 2011-06-08 | 广东省昆虫研究所 | Method for breeding Chinese rana in Yuebei |
| CN104381213A (en) * | 2014-11-14 | 2015-03-04 | 劳炳凤 | High-density frog breeding method |
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2016
- 2016-06-16 CN CN201610424927.7A patent/CN106035215B/en active Active
Non-Patent Citations (1)
| Title |
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| 龙虱对高原林蛙蝌蚪生长和存活率的影响;阿舍小虎等;《广东农业科学》;20130831(第8期);P125－127 * |
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| CN106035215A (en) | 2016-10-26 |
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