CN106010563A - Aniline contaminated soil remediation agent - Google Patents
Aniline contaminated soil remediation agent Download PDFInfo
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- CN106010563A CN106010563A CN201610438219.9A CN201610438219A CN106010563A CN 106010563 A CN106010563 A CN 106010563A CN 201610438219 A CN201610438219 A CN 201610438219A CN 106010563 A CN106010563 A CN 106010563A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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Abstract
The invention belongs to the technical field of soil remediation, and particularly relates to an aniline contaminated soil remediation agent. The aniline contaminated soil remediation agent is prepared from chitin, zeolite powder, green alga, humus acid, shell powder, needle mushroom residues, cobs, rice bran, tea seed shells, peat, areca catechu wood bits, palm wood bits, a complex enzyme preparation and a compound microorganism microbial agent. By the adoption of the soil remediation agent, under the condition of not introducing other chemical solvents, mild enzymes and microorganism strains are adopted for treating soil, no secondary contamination is generated, and the acting conditions are mild.
Description
Technical field
The invention belongs to technical field of soil remediation, be specifically related to a kind of phenyl amines contaminated soil remediation agent.
Background technology
Aniline is a kind of important industrial chemicals, is also a kind of poisonous chemical substance, and such material mainly comes
Come from the art production process, especially printing and dyeing mill such as printing and dyeing, pharmacy, rubber, weaving, papermaking, delft
The waste water of the discharge concentration containing aniline category matter is higher than the dyeing waste water discharge standard of national regulation, and these waste water are such as
Fruit is discharged in soil, necessarily brings serious soil pollution.
Aniline belongs to high toxic material, is combined into metahemoglobin with hemoglobin, is allowed to and oxygen binding ability
Declining causes animal to be poisoned, and long-term contact with such material also can cause hepatocarcinoma or bladder cancer, by sucking and inhaling
Receipts approach, such material may result in human body acute poisoning or chronic poisoning, produces headache, dizziness, fatigue, note
Recall the symptoms such as power decline, hypopnea even death by suffocation.Therefore Nitrobenzol and aniline are listed in Environment Priority control
Toxic organic pollutant processed.Microorganism remediation technology is to pollute one of effective method of environmental improvement, wherein to can
The separation of the bacterial strain of fast degradation pollutant, to cultivate and apply be the important step of microorganism remediation process, in recent years
Come separated to multiple can the microorganism fungus kind of degrading nitrobenzene, including pseudomonas, comamonas, class
Bacillus etc..The bacterial strain of aniline degradation has Shewanella etc., but major part bacterial strain is the most inclined to organic degradation rate
Low, degradation process requires harshness to environmental factors such as temperature, pH and other biological factor etc., puts into pollution
Behind place, adaptive time is longer and competitiveness is relatively low, and have needs through cometabolism process ability degrading nitrobenzene,
Thus greatly limit they application in pollution law.
Activate indigenous microorganism and mainly significantly improve biological activity by aeration, additional nutritive salt or substrate,
Promote the biodegradation of persistent organic pollutants.BROWN etc. utilize plant terpene compound to activate indigenous micro-life
Thing degraded trichloro ethylene, wherein adds in the water sample of linalool and carvone, and the degradation rate of trichloro ethylene is higher than
88%.YU etc. find, add the indigenous microorganism after mineral salt to the degradation rate of polycyclic aromatic hydrocarbon in substrate more than 97%.
Chitosan is added in the substrate applying slow-release fertilizer by XU etc., the indigenous microorganism PAHs degradation rate to 4-6 ring
When being not added with chitosan than matched group, degradation rate improves 3-4 times.
Indigenous microorganism carries out Nitrobenzol, the research of aniline pollution amelioration at home and abroad rarely has report to utilize activation.
Accordingly, it would be desirable to for the feature of aniline, grope a kind of remove aniline effective and relatively mild do not produce
The contaminated soil renovation agent of tissue regeneration promoting.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides one and imitate being administered by phenyl amines contaminated soil
Fruit is good and action condition gentleness the most do not bring the renovation agent of new pollution, this soil-repairing agent combine plant, enzyme and
Microorganism acts on jointly by aniline contaminated soil, makes the aniline in soil or absorption or is chelated or is dropped
Solve, reach to administer the purpose of phenyl amines contaminated soil.
The present invention is to be realized by following technical scheme:
Phenyl amines contaminated soil renovation agent, including the component of following portions by weight:
Compound enzymic preparation includes polyphenol oxidase, peroxidase, nitroreductase；
The enzyme of polyphenol oxidase alive 5.4 × 105U/g；The enzyme of peroxidase alive 18.3 × 105U/g；Nitro
The enzyme of reductase alive 8.9 × 105U/g；Polyphenol oxidase, peroxidase, the parts by weight of nitroreductase
Ratio is 1-4:2-6:1-5；
Complex micro organism fungicide 0.005-0.048；
The active component of above-mentioned complex micro organism fungicide is by Shewanella mycopowder, Bacillus licheniformis powder, curved
Bent pseudomonas mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, Ralstonia bacterium mycopowder are formed；
Above-mentioned Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast
Mycopowder, whiterot fungi mycopowder, the weight ratio of Ralstonia bacterium mycopowder be: (1-4): (1-5): (2-5): (1-6):
(1-5): (2-5)；
The living bacteria count of Shewanella mycopowder is 2 × 108-9×109cfu/g；
The living bacteria count of Bacillus licheniformis powder is 4 × 109-9×1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 5 × 108-9×109cfu/g；
The living bacteria count of Pichia yeast mycopowder is 2.5 × 109-9×1010cfu/g；
The living bacteria count of whiterot fungi mycopowder is 3.5 × 109-9×1011cfu/g；
The living bacteria count of Ralstonia bacterium mycopowder is 5.5 × 108-9×109cfu/g。
Preferably, the weight ratio of above-mentioned each mycopowder is:
Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder,
Whiterot fungi mycopowder, the weight ratio of Ralstonia bacterium mycopowder be: 3:3:4:3:3:4；
Compound enzymic preparation is 0.008 part；
Complex micro organism fungicide 0.036 part.
It is furthermore preferred that the soil-repairing agent of the present invention includes the component of following portions by weight:
Compound enzymic preparation includes polyphenol oxidase, peroxidase, nitroreductase；
The enzyme of polyphenol oxidase alive 5.4 × 105U/g；Peroxidase 18.3 × 105U/g；Nitroreductase
8.9×105U/g；Polyphenol oxidase, peroxidase, the ratio of weight and number of nitroreductase are 2:4:3；
Complex micro organism fungicide 0.036；
The active component of complex micro organism fungicide is by Shewanella mycopowder, Bacillus licheniformis powder, bending vacation list
Born of the same parents' bacterium mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, Ralstonia bacterium mycopowder are formed；
Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder,
Whiterot fungi mycopowder, the weight ratio of Ralstonia bacterium mycopowder be: 3:3:4:3:3:4；
The living bacteria count of Shewanella mycopowder is 2 × 108-9×109cfu/g；
The living bacteria count of Bacillus licheniformis powder is 4 × 109-9×1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 5 × 108-9×109cfu/g；
The living bacteria count of Pichia yeast mycopowder is 2.5 × 109-9×1010cfu/g；
The living bacteria count of whiterot fungi mycopowder is 3.5 × 109-9×1011cfu/g；
The living bacteria count of Ralstonia bacterium mycopowder is 5.5 × 108-9×109cfu/g。
Preferably, soil-repairing agent includes the component of following portions by weight:
Compound enzymic preparation is made up of polyphenol oxidase, peroxidase, nitroreductase, polyphenol oxidase；
The enzyme of polyphenol oxidase alive 5.4 × 105U/g；The enzyme of peroxidase alive 18.3 × 105U/g；Nitro
The enzyme of reductase alive 8.9 × 105U/g, the enzyme work of polyphenol oxidase are 11.3 × 105U/g；Polyphenol oxidase,
Peroxidase, nitroreductase, the ratio of weight and number of polyphenol oxidase are 2:5:2:3；
Complex micro organism fungicide 0.036 part；
The active component of complex micro organism fungicide is by Shewanella mycopowder, Bacillus licheniformis powder, bending vacation list
Born of the same parents' bacterium mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, Ralstonia bacterium mycopowder are formed；
Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder,
Whiterot fungi mycopowder, the weight ratio of Ralstonia bacterium mycopowder be: 3:3:4:3:3:4；
The living bacteria count of Shewanella mycopowder is 8 × 109cfu/g；
The living bacteria count of Bacillus licheniformis powder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of Pichia yeast mycopowder is 5 × 1010cfu/g；
The living bacteria count of whiterot fungi mycopowder is 5 × 1011cfu/g；
The living bacteria count of Ralstonia bacterium mycopowder is 6 × 109cfu/g；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Peat composed of rotten mosses ash is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized.
It is furthermore preferred that phenyl amines contaminated soil renovation agent, including the component of following portions by weight:
Mix with pedotheque, keep 48 hours；
Mycopowder of the present invention, enzyme are derived from commercially available；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Peat composed of rotten mosses ash is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized；
Add complex micro organism fungicide 0.036 part, mix homogeneously；
The active component of complex micro organism fungicide is by Shewanella mycopowder, Bacillus licheniformis powder, bending vacation list
Born of the same parents' bacterium mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, Ralstonia bacterium mycopowder are formed；
Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder,
Whiterot fungi mycopowder, the weight ratio of Ralstonia bacterium mycopowder be: 3:3:4:3:3:4；
The living bacteria count of Shewanella mycopowder is 8 × 109cfu/g；
The living bacteria count of Bacillus licheniformis powder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of Pichia yeast mycopowder is 5 × 1010cfu/g；
The living bacteria count of whiterot fungi mycopowder is 5 × 1011cfu/g；
The living bacteria count of Ralstonia bacterium mycopowder is 6 × 109cfu/g。
The beneficial effects of the present invention is, use the soil-repairing agent of the present invention, do not introducing other chemistry
Under conditions of solvent, use gentle enzyme and microorganism fungus kind that soil is processed, do not bring new pollution into,
Action condition is gentle, and strain effectively degrades aniline.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described, in order to those skilled in the art
Know more about the present invention, but and be not so limited the present invention.
Embodiment 1
Take by phenyl amines contaminated soil sample, then join soil-repairing agent 50 grams by every 1000 grams of pedotheques
Ratio fetch earth soil repair agent；
In pedotheque, add following raw material: chitin, zeolite powder, chlorella, humic acid, conch meal,
Flammulina velutiper (Fr.) Sing mushroom bran, corn cob, flyash, tea seed episperm, peat composed of rotten mosses ash, betelnut tree wood flour, palm wood flour；
The parts by weight of each raw material are as follows:
Mix with pedotheque, keep 48 hours；
Mycopowder of the present invention, enzyme are derived from commercially available；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Peat composed of rotten mosses ash is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized；
Add complex micro organism fungicide 0.036 part, mix homogeneously；
The active component of complex micro organism fungicide is by Shewanella mycopowder, Bacillus licheniformis powder, bending vacation list
Born of the same parents' bacterium mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, Ralstonia bacterium mycopowder are formed；
Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder,
Whiterot fungi mycopowder, the weight ratio of Ralstonia bacterium mycopowder be: 3:3:4:3:3:4；
The living bacteria count of Shewanella mycopowder is 8 × 109cfu/g；
The living bacteria count of Bacillus licheniformis powder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of Pichia yeast mycopowder is 5 × 1010cfu/g；
The living bacteria count of whiterot fungi mycopowder is 5 × 1011cfu/g；
The living bacteria count of Ralstonia bacterium mycopowder is 6 × 109cfu/g；
Above living bacteria count is approximate number, as follows；
Being eventually adding compound enzymic preparation 0.008 part, mix homogeneously, compound enzymic preparation is by polyphenol oxidase, peroxide
Compound enzyme, nitroreductase, phytase are formed, and the enzyme of polyphenol oxidase is lived and is about 5.4 × 105U/g；Cross
The enzyme of oxide enzyme is lived and is about 18.3 × 105U/g；The enzyme of nitroreductase is lived and is about 8.9 × 105U/g, plant
The enzyme of acid enzyme is lived and is about 11.3 × 105U/g；Polyphenol oxidase, peroxidase, nitroreductase, phytase
Ratio of weight and number be 2:4:3:2；
This compound enzymic preparation first stirs in the water of 45 DEG C, is then sprayed in pedotheque, and will
Pedotheque stirs；
Above " part " is parts by weight, and following example are same, if no special instructions.
Inventor has been again following contrast experiment about the application, specific as follows:
Comparative example 1
With not being both of embodiment 1, in comparative example 1 and be provided without compound enzymic preparation, remaining is identical；
Comparative example 2
With not being both of embodiment 1, in comparative example 1 and be provided without complex micro organism fungicide, remaining complete phase
With；
Comparative example 3
With not being both of embodiment 1, complex micro organism fungicide is different, the concrete complex micro organism fungicide used
As follows:
Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder,
The weight ratio of Ralstonia bacterium mycopowder is: 3:4:3:3:4；
Comparative example 4
With not being both of embodiment 1, compound enzymic preparation is polyphenol oxidase, peroxidase is formed answers
Synthase, the enzyme work of polyphenol oxidase is 5.4 × 105U/g；The work of peroxidase enzyme is 18.3 × 105U/g；
Polyphenol oxidase, the ratio of weight and number of peroxidase are 3:2.
Comparative example 5
As different from Example 1, it is provided without complex micro organism fungicide and compound enzymic preparation, remaining complete phase
With.When processing soil, and after pedotheque mixing, keep 48 hours.
Pedotheque and renovation agent being placed in the container of a cuboid, pedotheque is laid about with renovation agent
20-25 centimetre is thick, pedotheque and soil-repairing agent is fully mixed by above-mentioned and protected by the above-mentioned time
After holding, then trickle in a reservoir, keep pedotheque and renovation agent to moisten；Drip washing in every 10 days is once；Water
Solution is drained by outlet with filter screen below container.When 1 month, 2 months time, 3 months time respectively
Survey the content of aniline in soil.Use the content of aniline, degrading aniline rate in Soil by Gas Chromatography sample
Computing formula:
Y=(M-W)/M × 100%
In formula, Y is degrading aniline rate, %；W is residue aniline quality, g in pedotheque；M is soil-like
Product Central Plains aniline quality, g.
Each comparative example is also adopted by above-mentioned processing mode, and result is as follows:
Degrading aniline rate in the pedotheque of table 1 embodiment 1 and comparative example 1-5
Embodiment 2
Identical with the pedotheque in embodiment 1, use the method identical with embodiment 1 to remove soil
Aniline in sample, is a difference in that the parts by weight etc. of soil-repairing agent are had any different；
Compound enzymic preparation is made up of polyphenol oxidase, peroxidase, nitroreductase；
The enzyme of polyphenol oxidase alive 5.4 × 105U/g；Peroxidase 18.3 × 105U/g；Nitroreductase
8.9×105U/g；Polyphenol oxidase, peroxidase, the ratio of weight and number of nitroreductase are 2:4:3:
1；
Complex micro organism fungicide 0.005；
The active component of above-mentioned complex micro organism fungicide is by Shewanella mycopowder, Bacillus licheniformis powder, curved
Bent pseudomonas mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, Ralstonia bacterium mycopowder are formed；
Above-mentioned Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast
Mycopowder, whiterot fungi mycopowder, the weight ratio of Ralstonia bacterium mycopowder be: 1:1:2:1:1:2；
Above-mentioned Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast
In mycopowder, whiterot fungi mycopowder, Ralstonia bacterium mycopowder；
The living bacteria count of Shewanella mycopowder is 8 × 109cfu/g；
The living bacteria count of Bacillus licheniformis powder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of Pichia yeast mycopowder is 5 × 1010cfu/g；
The living bacteria count of whiterot fungi mycopowder is 5 × 1011cfu/g；
The living bacteria count of Ralstonia bacterium mycopowder is 6 × 109cfu/g；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Peat composed of rotten mosses ash is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized.
Embodiment 3
Identical with the pedotheque in embodiment 1, use the method identical with embodiment 1 to remove soil
Aniline in sample, is a difference in that the parts by weight etc. of soil-repairing agent are had any different；
Compound enzymic preparation is polyphenol oxidase, peroxidase, nitroreductase；
The enzyme of polyphenol oxidase alive 5.4 × 105The work of U/g, peroxidase enzyme is 18.3 × 105U/g, nitro
The work of reductase enzyme is 8.9 × 105U/g；
The ratio of weight and number of polyphenol oxidase, peroxidase and nitroreductase is 1:2:1；
Complex micro organism fungicide 0.048；
The active component of complex micro organism fungicide is by Shewanella mycopowder, Bacillus licheniformis powder, bending vacation list
Born of the same parents' bacterium mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, Ralstonia bacterium mycopowder are formed；
Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder,
Whiterot fungi mycopowder, the weight ratio of Ralstonia bacterium mycopowder be: 4:5:5:6:5:5；
The living bacteria count of Bacillus licheniformis powder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of Pichia yeast mycopowder is 5 × 1010cfu/g；
The living bacteria count of whiterot fungi mycopowder is 5 × 1011cfu/g；
The living bacteria count of Ralstonia bacterium mycopowder is 6 × 109cfu/g；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Peat composed of rotten mosses ash is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized；
Embodiment 4
Identical with the pedotheque in embodiment 1, use the method identical with embodiment 1 to remove soil
Aniline in sample, is a difference in that the parts by weight etc. of soil-repairing agent are had any different；
Soil-repairing agent includes the component of following portions by weight:
Compound enzymic preparation is made up of polyphenol oxidase, peroxidase, nitroreductase, phytase；
The enzyme of polyphenol oxidase alive 5.4 × 105U/g；The enzyme of peroxidase alive 18.3 × 105U/g；Nitro
The enzyme of reductase alive 8.9 × 105U/g, the enzyme work of phytase are 11.3 × 105U/g；Polyphenol oxidase, peroxide
Compound enzyme, nitroreductase, the ratio of weight and number of phytase are 2:5:2:3；
Complex micro organism fungicide 0.036 part；
The active component of complex micro organism fungicide is by Shewanella mycopowder, Bacillus licheniformis powder, bending vacation list
Born of the same parents' bacterium mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, Ralstonia bacterium mycopowder are formed；
Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder,
Whiterot fungi mycopowder, the weight ratio of Ralstonia bacterium mycopowder be: 3:3:4:3:3:4；
The living bacteria count of Shewanella mycopowder is 8 × 109cfu/g；
The living bacteria count of Bacillus licheniformis powder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of Pichia yeast mycopowder is 5 × 1010cfu/g；
The living bacteria count of whiterot fungi mycopowder is 5 × 1011cfu/g；
The living bacteria count of Ralstonia bacterium mycopowder is 6 × 109cfu/g；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Peat composed of rotten mosses ash is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized.
Embodiment 5
Identical with the pedotheque in embodiment 1, use the method identical with embodiment 1 to remove soil
Aniline in sample, is a difference in that the parts by weight etc. of soil-repairing agent are had any different；
Compound enzymic preparation includes polyphenol oxidase, peroxidase, nitroreductase；
The enzyme of polyphenol oxidase alive 5.4 × 105U/g；Peroxidase 18.3 × 105U/g；Nitroreductase
8.9×105U/g；Polyphenol oxidase, peroxidase, the ratio of weight and number of nitroreductase are 2:4:3；
The active component of complex micro organism fungicide is by Shewanella mycopowder, Bacillus licheniformis powder, bending vacation list
Born of the same parents' bacterium mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, Ralstonia bacterium mycopowder are formed；
Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder,
Whiterot fungi mycopowder, the weight ratio of Ralstonia bacterium mycopowder be: 3:3:4:3:3:4；
The living bacteria count of Shewanella mycopowder is 5 × 109cfu/g；
The living bacteria count of Bacillus licheniformis powder is 4 × 1010cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 6 × 109cfu/g；
The living bacteria count of Pichia yeast mycopowder is 6 × 1010cfu/g；
The living bacteria count of whiterot fungi mycopowder is 5 × 1011cfu/g；
The living bacteria count of Ralstonia bacterium mycopowder is 7 × 109cfu/g。
After embodiment 2-5 all uses method same as in Example 1 to process, record aniline in pedotheque
Degradation rate result is as follows:
| 1 month | 2 months | 3 months | |
| Embodiment 2 | 19% | 40% | 63% |
| Embodiment 3 | 20% | 47% | 66% |
| Embodiment 4 | 22% | 46% | 67% |
| Embodiment 5 | 21% | 45% | 66% |
Claims (7)
1. phenyl amines contaminated soil renovation agent, including the component of following portions by weight:
Chitin 1-3
Zeolite powder 80-200
Chlorella 10-25 humic acid 0.5-2
Conch meal 2-9 Flammulina velutiper (Fr.) Sing mushroom bran 5-15
Corn cob 40-80
Flyash 10-20
Tea seed episperm 5-15 peat composed of rotten mosses ash 5-20
Betelnut tree wood flour 20-80 palm wood flour 25-60
Compound enzymic preparation 0.001-0.02；
Described compound enzymic preparation includes polyphenol oxidase, peroxidase, nitroreductase；
The enzyme of described polyphenol oxidase alive 5.4 × 105U/g；The enzyme of peroxidase alive 18.3 × 105U/g ；The enzyme of nitroreductase alive 8.9 × 105U/g；Polyphenol oxidase, peroxidase, the ratio of weight and number of nitroreductase are 1-4:2-6:1-5；
Complex micro organism fungicide 0.005-0.048；
The active component of described complex micro organism fungicide is made up of Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, Ralstonia bacterium mycopowder；
Described Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, the weight ratio of Ralstonia bacterium mycopowder be: (1-4): (1-5): (2-5): (1-6): (1-5): (2-5)；
The living bacteria count of described Shewanella mycopowder is 2 × 108-9×109cfu/g；
The living bacteria count of Bacillus licheniformis powder is 4 × 109-9×1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 5 × 108-9×109cfu/g；
The living bacteria count of Pichia yeast mycopowder is 2.5 × 109-9×1010cfu/g；
The living bacteria count of whiterot fungi mycopowder is 3.5 × 109-9×1011cfu/g；
The living bacteria count of Ralstonia bacterium mycopowder is 5.5 × 108-9×109cfu/g。
2. phenyl amines contaminated soil renovation agent as claimed in claim 1, it is characterised in that the weight ratio of each mycopowder is:
Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, the weight ratio of Ralstonia bacterium mycopowder be: 3:3:4:3:3:4.
3. phenyl amines contaminated soil renovation agent as claimed in claim 1, it is characterised in that described compound enzymic preparation is 0.008 part.
4. phenyl amines contaminated soil renovation agent as claimed in claim 1, it is characterised in that described complex micro organism fungicide 0.036 part.
5. phenyl amines contaminated soil renovation agent as claimed in claim 1, it is characterised in that include the component of following portions by weight:
Chitin 2
Zeolite powder 160
Chlorella 20 humic acid 1.5
Conch meal 6
Flammulina velutiper (Fr.) Sing mushroom bran 10
Corn cob 60
Flyash 15
Tea seed episperm 10
Peat composed of rotten mosses ash 15
Betelnut tree wood flour 60
Palm wood flour 45
Compound enzymic preparation 0.008
Complex micro organism fungicide 0.036；
Described compound enzymic preparation includes polyphenol oxidase, peroxidase, nitroreductase；
The enzyme of described polyphenol oxidase alive 5.4 × 105U/g；Peroxidase 18.3 × 105U/g ；Nitroreductase 8.9 × 105U/g；Polyphenol oxidase, peroxidase, the ratio of weight and number of nitroreductase are 2:4:3；
The active component of described complex micro organism fungicide is made up of Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, Ralstonia bacterium mycopowder；
Described Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, the weight ratio of Ralstonia bacterium mycopowder be: 3:3:4:3:3:4；
The living bacteria count of Shewanella mycopowder is 2 × 108-9×109cfu/g；
The living bacteria count of Bacillus licheniformis powder is 4 × 109-9×1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 5 × 108-9×109cfu/g；
The living bacteria count of Pichia yeast mycopowder is 2.5 × 109-9×1010cfu/g；
The living bacteria count of whiterot fungi mycopowder is 3.5 × 109-9×1011cfu/g；
The living bacteria count of Ralstonia bacterium mycopowder is 5.5 × 108-9×109cfu/g。
6. phenyl amines contaminated soil renovation agent as claimed in claim 1, it is characterised in that include the component of following portions by weight:
Soil-repairing agent includes the component of following portions by weight:
Chitin 2.5
Zeolite powder 160
Chlorella 18
Humic acid 1.5
Conch meal 7
Flammulina velutiper (Fr.) Sing mushroom bran 12
Corn cob 60
Flyash 16
Tea seed episperm 12
Peat composed of rotten mosses ash 18
Betelnut tree wood flour 60
Palm wood flour 50
Compound enzymic preparation 0.012；
Compound enzymic preparation is made up of polyphenol oxidase, peroxidase, nitroreductase, phytase；
The enzyme of polyphenol oxidase alive 5.4 × 105U/g；The enzyme of peroxidase alive 18.3 × 105U/g ；The enzyme of nitroreductase alive 8.9 × 105U/g, the enzyme work of phytase are 11.3 × 105U/g；
Polyphenol oxidase, peroxidase, nitroreductase, the ratio of weight and number of phytase are 2:5:2:3；
Complex micro organism fungicide 0.036 part；
The active component of complex micro organism fungicide is made up of Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, Ralstonia bacterium mycopowder；
Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, the weight ratio of Ralstonia bacterium mycopowder be: 3:3:4:3:3:4；
The living bacteria count of Shewanella mycopowder is 8 × 109cfu/g；
The living bacteria count of Bacillus licheniformis powder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of Pichia yeast mycopowder is 5 × 1010cfu/g；
The living bacteria count of whiterot fungi mycopowder is 5 × 1011cfu/g；
The living bacteria count of Ralstonia bacterium mycopowder is 6 × 109cfu/g；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is 4%；
Peat composed of rotten mosses ash is also for shining the powder after dry doubling is pulverized, and its water content is 3%；
Betelnut tree wood flour, palm wood flour be dry to moisture be the bits shape thing pulverized after 4%.
7. phenyl amines contaminated soil renovation agent as claimed in claim 1, it is characterised in that include the component of following portions by weight:
Chitin 2
Zeolite powder 160
Chlorella 20
Humic acid 1.5
Conch meal 6
Flammulina velutiper (Fr.) Sing mushroom bran 10
Corn cob 60
Flyash 15
Tea seed episperm 10
Peat composed of rotten mosses ash 15
Betelnut tree wood flour 60
Palm wood flour 45；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is 4%；
The peat composed of rotten mosses its water content of ash is 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized；
Complex micro organism fungicide 0.036 part,
The active component of complex micro organism fungicide is made up of Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, Ralstonia bacterium mycopowder；
Shewanella mycopowder, Bacillus licheniformis powder, Pseudomonas geniculate mycopowder, Pichia yeast mycopowder, whiterot fungi mycopowder, the weight ratio of Ralstonia bacterium mycopowder be: 3:3:4:3:3:4；
The living bacteria count of Shewanella mycopowder is 8 × 109cfu/g；
The living bacteria count of Bacillus licheniformis powder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of Pichia yeast mycopowder is 5 × 1010cfu/g；
The living bacteria count of whiterot fungi mycopowder is 5 × 1011cfu/g；
The living bacteria count of Ralstonia bacterium mycopowder is 6 × 109cfu/g；
Compound enzymic preparation 0.008；
Compound enzymic preparation includes polyphenol oxidase, peroxidase, nitroreductase；
The enzyme of polyphenol oxidase alive 5.4 × 105U/g；Peroxidase 18.3 × 105U/g ；Nitroreductase 8.9 × 105U/g；Polyphenol oxidase, peroxidase, the ratio of weight and number of nitroreductase are 2:4:3.
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| CN201610438219.9A CN106010563A (en) | 2016-06-17 | 2016-06-17 | Aniline contaminated soil remediation agent |
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| CN201610438219.9A CN106010563A (en) | 2016-06-17 | 2016-06-17 | Aniline contaminated soil remediation agent |
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