CN106007886A - Culture material of Ganoderma tsugae suitable for cultivation in northeast and cultivation method thereof - Google Patents
Culture material of Ganoderma tsugae suitable for cultivation in northeast and cultivation method thereof Download PDFInfo
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- CN106007886A CN106007886A CN201610085166.7A CN201610085166A CN106007886A CN 106007886 A CN106007886 A CN 106007886A CN 201610085166 A CN201610085166 A CN 201610085166A CN 106007886 A CN106007886 A CN 106007886A
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Abstract
The invention relates to a culture material of Ganoderma tsugae suitable for cultivation in the northeast. The culture material comprises Ganoderma tsugae first level strain culture material and a Ganoderma tsugae second level strain culture material. The Ganoderma tsugae first level strain culture material consists of, by weight, 150-250 parts of potato, 10-25 parts of glucose, 14-25 parts of agar powder, 2-4 parts of potassium dihydrogen phosphate, 1-2 parts of magnesium sulfate, 0.5-1.5 parts of peptone, and 1000 parts of water. The adding of broadleaf sawdust and pine sawdust into the Ganoderma tsugae second level strain culture material has an obvious promoting effect on the growth of Ganoderma tsugae's secondary mycelia, speeds up the growth process of Ganoderma tsugae, shortens the growth period of Ganoderma tsugae, effectively improves the yield of Ganoderma tsugae, and enhances the Ganoderma tsugae's quality against external bad environment, so that the nutritional value of the developed Ganoderma tsugae can be significantly improved.
Description
Technical field
The present invention relates to the cultivation of a kind of Ganoderma tsugae, be specifically related to a kind of Ganoderma tsugae compost being suitable to the Northeast's cultivation and cultural method thereof.
Background technology
On larch that Ganoderma tsugae is grown in mixed coniferous broad leaved forest and the stump of PiceameyeriRehd. Et Wils. and corruption wood, it is distributed in China cold temperate regions, gathers autumn.Ganoderma tsugae is one of probiotic bacteria of health ministry announcement, one of distinctive rare medicinal fungus in Ye Shi the Northeast.
Generation research proves, Ganoderma has many pharmacological actions, and by finding the research of Ganoderma tsugae medical value, one of composition that Ganoderma tsugae sporophore is most precious is organic germanium, is 4～6 times of Radix Ginseng, i.e. 800～1000m parts/k part.Germanium can make blood circulation unimpeded, strengthens erythrocyte and transports the ability of oxygen, enhances metabolism, slow down aging, and can combine with internal pollution thing, heavy metal and become the Organic substance of germanium and excrete.Ganoderma tsugae contains macromolecule polysaccharide.It can strengthen the immunocompetence of people, improves the human body resistivity to disease, cures the disease to retransmit in anti-cancer and waves good effect.As the many-sides such as central nervous system, respiratory system, cardiovascular system and liver, smooth muscle, endocrine and immune system being had treatment or regulatory function.Ganoderma is applied the most clinically, treat the diseases such as chronic bronchitis, coronary heart disease, hyperlipidemia, leukopenia, aplastic anemia, hepatitis, Keshan disease, muscular dystrophy, alopecia areata, neurasthenia, cancer being used for, all achieve preferable curative effect, general obvious effective rate is about 39%, and total effective rate is 70%～90%.Ganoderma is mainly the function by improving body immune system to the therapeutical effect of various diseases, strengthens what human disease resistance reached.Owing to wild Ganoderma is little, and artificial culture is limited to summer, autumn more, therefore Ganoderma yield has the gesture that supply falls short of demand at present, and therefore, the method that research improves Ganoderma yield is necessary.
Due to people's enhancing to health care consciousness, wild Ganoderma tsugae is also in the artificial unordered harvesting stage, and forest land wild Ganoderma tsugae natural production is relatively low, and unordered harvesting causes Ganoderma tsugae of poor quality, and product specification is low, causes wild resource increasingly to reduce.By cultivating excellent Ganoderma tsugae strain, carry out artificial culture and original ecology cultivating, wild resource in improving the Yield and quality of Ganoderma tsugae and forest reserve is had positive effect.
Utilize artificial vaccination, under manually operated indoor environment, making Ganoderma obtain more preferable conditions of growth and development is the cultivation technique just grown up in nearly more than 40 years, mainly has: (1) indoors artificial bottle cultivation technology: this method is generalized to all parts of the country at the initial stage seventies, the most still in application.(2) technology planted by indoors artificial bag: is the cultural method utilizing special high temperature resistant, high pressure and nontoxic plastic bag to replace vial, starts from the end of the seventies, the most still in application.But traditional ganoderma lucidum cultivation method yields poorly, though the critical technical parameter about indoors artificial cultivating ganoderma has a lot of report on all kinds of books and periodicals, but the critical technical parameter of its display is inconsistent, and the test to various technical parameters is all single factor test comparative test, the most systematically, all sidedly to the critical technical parameter of method, the process of Ganoderma growth and the Ganoderma growth stage of cultivation of glossy ganoderma test.
Daxinganling District is one of major production areas of Ganoderma tsugae, current tame quantity is less, and yield poorly (average product 12 parts～26 parts/bag), cycle length, poor quality (deformity sesame), resistance weak (being easily bacterial contamination in cultivation) and etc. problem, had a strong impact on the development of mushroom industry.
The present invention one strain is suitable to the Ganoderma tsugae strain D002 of the Northeast's cultivation and identified belongs to Ganoderma novel species, it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date is on May 10th, 2013, and deposit number is CGMCC7605.D002 selects excellent wild Ganoderma tsugae sporophore as material with A Moore, Daxinganling District forestry bureau according to woods forest farm, carries out separate tissue, obtains Pure cultured spawn after purification.Tame through artificial culture, determine the merit of D002, and carry out Regional Cultural Experiment in different regions, determine the D002 adaptability to the Northeast's weather and the stability of shape.
Summary of the invention
The present invention provides a kind of Ganoderma tsugae compost being suitable to the Northeast's cultivation and cultural method thereof, the Ganoderma tsugae strain that the invention aims to solve use in existing production also exist yield poorly, poor quality and the weak problem of resistance, and provide a kind of Ganoderma tsugae compost being suitable to the Northeast's cultivation and cultural method thereof, to solve problems of the prior art.
For achieving the above object, by the following technical solutions:
A kind of Ganoderma tsugae compost being suitable to the Northeast's cultivation, including Ganoderma tsugae first class inoculum compost and Ganoderma tsugae second class inoculum compost, including Ganoderma tsugae first class inoculum compost and Ganoderma tsugae second class inoculum compost, described Ganoderma tsugae first class inoculum compost is Rhizoma Solani tuber osi 150～250 parts, glucose 10～25 parts, agar powder 14～25 parts, potassium dihydrogen phosphate 2～4 parts, magnesium sulfate 1～2 parts, peptone 0.5～1.5 parts, the parts by weight configuration of 1000 parts of water；The compost of described Ganoderma tsugae second class inoculum by broad-leaved wood flour 70～80%, pinaster wood flour 5～10%, Testa Tritici 13～20%, Gypsum Fibrosum 1～2%, Semen Glycines powder 1～3% composition.
A kind of method of Ganoderma tsugae compost cultivation Ganoderma tsugae being suitable to the Northeast's cultivation, comprises the following steps,
(1) strain separating:
Select wild Ganoderma tsugae sporophore, in workbench, with 70～75% alcohol wipe Ganoderma tsugae sporophore surface, the tissue in picking stem and cap joint portion, access rapidly in inclined-plane, put in 15～30 DEG C of constant incubators and cultivate, routine observation, by microscopy, selects the strain grown fine, become first class inoculum standby, eliminate and pollute strain；
(2) first class inoculum culture medium makes and first class inoculum inoculation；
The making of first class inoculum culture medium is chosen the parts by weight of Rhizoma Solani tuber osi 150～250 parts, glucose 10～25 parts, agar powder 14～25 parts, potassium dihydrogen phosphate 2～4 parts, magnesium sulfate 1～2 parts, peptone 0.5～1.5 parts, 1000 parts of water and is configured, Rhizoma Solani tuber osi is through peeling, stripping and slicing, liquor, sieve the mixing that adds water, access first class inoculum after tubulature, sterilizing, cooling, cultivate good rear low-temperature preservation standby；
(3) making of second class inoculum culture medium:
Weigh broad-leaved wood flour 70～80%, pinaster wood flour 5～10%, Testa Tritici 13～20%, Gypsum Fibrosum 1～2% by mass percentage, Semen Glycines powder 1～3%, sieve the mixing that adds water, make water content 60%～65%, mix thoroughly, load in the Polythene Bag of 16.5cm × 33cm specification, every packed culture medium wet feed 1.2～1.3k part, use the sealing of bacterium rod to put upside down, form the bacterium bag equipped with second class inoculum compost；
(4) sterilizing and inoculation:
By the bacterium bag equipped with second class inoculum culture medium produced in step (3), use normal pressure moist heat sterilization, sterilizing 8h at 100 DEG C, standby, being accessed by first class inoculum produced in step (2) in the bacterium bag equipped with second class inoculum compost after sterilizing, a test tube first class inoculum connects 6～10 second class inoculum bags；
(5) condition of culture:
By bacterium bag postvaccinal in step (4), it is positioned over indoor cultivation, room temperature 15～30 DEG C, relative air humidity natural drying, indoor lucifuge, well-ventilated, after the long purseful of mycelia, under the conditions of room temperature 15～20 DEG C, is further cultured for 30～40d；It is made to complete the physiological process of mycelia after-ripening；
(6) go out sesame to manage:
When outdoor temperature reaches more than 5 DEG C, the bacterium bag cultivated in step (5) is buckled canopy and shelters from heat or light, and is removed by the tampon of bacterium bag mouth, put ground, highly 12～15 layers, to pendulum at the bottom of bag, line space 50cm, ceiling installs equipment of sprinkler irrigation, and initial stage temperature of shed keeps 15～25 DEG C, and humidity keeps 70%～85%, after the former base of Ganoderma tsugae is formed, temperature of shed keeps 25～30 DEG C, and humidity keeps 85%～95%, and 35～42d can gather dry in the sun；
(7) gather:
When cap edge becomes bronzing, can gather, remove stem root impurity and be placed on gauze, daylight natural drying.
Beneficial effects of the present invention: due to the fact that and add agar powder, potassium dihydrogen phosphate, magnesium sulfate, peptone in Ganoderma tsugae first class inoculum compost, make in Ganoderma tsugae first class inoculum compost containing phosphate, sulfate and the composition of peptone, its nutritional labeling more horn of plenty, advantageously in the growth of mycelia, it is effectively improved the quality of one-level mycelia, being that the g and D of two grades of mycelia is had laid a good foundation, the growth for Ganoderma tsugae has obvious facilitation；Owing to adding broad-leaved wood flour and pinaster wood flour in Ganoderma tsugae second class inoculum compost, growth for two grades of mycelia of Ganoderma tsugae has obvious facilitation, accelerate the growth course of Ganoderma tsugae, shorten the Plant period of Ganoderma tsugae, effectively raise the yield of Ganoderma tsugae, and enhance Ganoderma tsugae and resist the quality of extraneous poor environment, make the nutritive value of the Ganoderma tsugae after growth obtain significant raising；The present invention also uses the tame first class inoculum of wild species source selection-breeding, makes the quality of Ganoderma tsugae be effectively improved.
Detailed description of the invention
In order to realize the object of the invention, the present invention provides the Ganoderma tsugae compost and cultural method thereof that a kind of the Northeast cultivates, the compost of the present invention, including Ganoderma tsugae first class inoculum compost and Ganoderma tsugae second class inoculum compost, Ganoderma tsugae first class inoculum compost is by Rhizoma Solani tuber osi 150～250 parts, glucose 10～25 parts, agar powder 14～25 parts, potassium dihydrogen phosphate 2～4 parts, magnesium sulfate 1～2 parts, peptone 0.5～1.5 parts, 1000 parts of weight proportions of water；The compost of Ganoderma tsugae second class inoculum by broad-leaved wood flour 70～80%, pinaster wood flour 5～10%, Testa Tritici 13～20%, Gypsum Fibrosum 1～2%, Semen Glycines powder 1～3% composition.
The technological process of the present invention: strain excellent → separate tissue → microscopy → first class inoculum → second class inoculum → dispensing → spice → pack → sterilizing → cooling inoculation → cultivate → enter canopy goes out sesame (or woodland is buried and stump connects bacterium) → management of watering → harvesting dry in the sun → packing and storing.
Implement process, such as following enforcement embodiment:
Embodiment 1:
Culture material formula:
Ganoderma tsugae first class inoculum compost is by Rhizoma Solani tuber osi 150 parts, glucose 10 parts, agar powder 14 parts, potassium dihydrogen phosphate 2 parts, 1 part of magnesium sulfate, peptone 0.5 part, 1000 parts of weight proportions of water；
Ganoderma tsugae second class inoculum compost is made up of broad-leaved wood flour 70%, pinaster wood flour 10%, Testa Tritici 15%, Gypsum Fibrosum 2%, Semen Glycines powder 3%.
A kind of method of Ganoderma tsugae compost cultivation Ganoderma tsugae being suitable to the Northeast's cultivation, comprises the following steps,
(1) strain separating:
Select wild Ganoderma tsugae sporophore, in workbench, with 70% alcohol wipe Ganoderma tsugae sporophore surface, the tissue in picking stem and cap joint portion, access rapidly in inclined-plane, put in 15 DEG C of constant incubators and cultivate, routine observation, by microscopy, selects the strain grown fine, become first class inoculum standby, eliminate and pollute strain；
(2) first class inoculum culture medium makes and first class inoculum inoculation；
First class inoculum culture medium makes and chooses Rhizoma Solani tuber osi 150 parts, glucose 25 parts, agar powder 14 parts, potassium dihydrogen phosphate 2 parts, 1 part of magnesium sulfate, peptone 0.5 part, 1000 parts of weight proportions of water, Rhizoma Solani tuber osi is through peeling, stripping and slicing, liquor, sieve the mixing that adds water, access first class inoculum after tubulature, sterilizing, cooling, cultivate good rear low-temperature preservation standby；
(3) making of second class inoculum culture medium:
Weigh broad-leaved wood flour 70%, pinaster wood flour 10%, Testa Tritici 15%, Gypsum Fibrosum 2%, Semen Glycines powder 3% by mass percentage, sieve the mixing that adds water, make water content 60%, mix thoroughly, load in the Polythene Bag of 16.5cm × 33cm specification, every packed culture medium wet feed 1.2k part, uses the sealing of bacterium rod to put upside down, forms the bacterium bag equipped with second class inoculum compost；
(4) sterilizing and inoculation:
By the bacterium bag equipped with second class inoculum culture medium produced in step (3), use normal pressure moist heat sterilization, sterilizing 8h at 100 DEG C, standby, being accessed by first class inoculum produced in step (2) in the bacterium bag equipped with second class inoculum compost after sterilizing, a test tube first class inoculum connects 6 second class inoculum bags；
(5) condition of culture:
By bacterium bag postvaccinal in step (4), it is positioned over indoor cultivation, room temperature 15 DEG C, relative air humidity natural drying, indoor lucifuge, well-ventilated, after the long purseful of mycelia, under the conditions of room temperature 15 DEG C, is further cultured for 30d；It is made to complete the physiological process of mycelia after-ripening；
(6) go out sesame to manage:
When outdoor temperature reaches more than 5 DEG C, the bacterium bag cultivated in step (5) is buckled canopy and shelters from heat or light, and is removed by the tampon of bacterium bag mouth, put ground, highly 12 layers, to pendulum at the bottom of bag, line space 50cm, ceiling installs equipment of sprinkler irrigation, and initial stage temperature of shed keeps 15 DEG C, and humidity keeps 70%, after the former base of Ganoderma tsugae is formed, temperature of shed keeps 25 DEG C, and humidity keeps 85%, 35d can gather dry in the sun；
(7) gather:
When cap edge becomes bronzing, can gather, remove stem root impurity and be placed on gauze, daylight natural drying.
Embodiment 2:
Culture material formula:
Ganoderma tsugae first class inoculum compost is by Rhizoma Solani tuber osi 250 parts, glucose 25 parts, agar powder 25 parts, potassium dihydrogen phosphate 4 parts, 2 parts of magnesium sulfate, peptone 1.5 parts, 1000 parts of weight proportions of water；
Ganoderma tsugae second class inoculum compost is made up of broad-leaved wood flour 80%, pinaster wood flour 5%, Testa Tritici 13%, Gypsum Fibrosum 1%, Semen Glycines powder 1%.
A kind of method of Ganoderma tsugae compost cultivation Ganoderma tsugae being suitable to the Northeast's cultivation, comprises the following steps,
(1) strain separating:
Select wild Ganoderma tsugae sporophore, in workbench, with 75% alcohol wipe Ganoderma tsugae sporophore surface, the tissue in picking stem and cap joint portion, access rapidly in inclined-plane, put in 30 DEG C of constant incubators and cultivate, routine observation, by microscopy, selects the strain grown fine, become first class inoculum standby, eliminate and pollute strain；
(2) first class inoculum culture medium makes and first class inoculum inoculation；
First class inoculum culture medium makes and chooses Rhizoma Solani tuber osi 250 parts, glucose 25 parts, agar powder 25 parts, potassium dihydrogen phosphate 4 parts, 2 parts of magnesium sulfate, peptone 1.5 parts, 1000 parts of weight proportions of water, Rhizoma Solani tuber osi is through peeling, stripping and slicing, liquor, sieve the mixing that adds water, access first class inoculum after tubulature, sterilizing, cooling, cultivate good rear low-temperature preservation standby；
(3) making of second class inoculum culture medium:
Weigh broad-leaved wood flour 80%, pinaster wood flour 5%, Testa Tritici 13%, Gypsum Fibrosum 1%, Semen Glycines powder 1% by mass percentage, sieve the mixing that adds water, make water content 63%, mix thoroughly, load in the Polythene Bag of 16.5cm × 33cm specification, every packed culture medium wet feed 1.3k part, uses the sealing of bacterium rod to put upside down, forms the bacterium bag equipped with second class inoculum compost；
(4) sterilizing and inoculation:
By the bacterium bag equipped with second class inoculum culture medium produced in step (3), use normal pressure moist heat sterilization, sterilizing 8h at 100 DEG C, standby, being accessed by first class inoculum produced in step (2) in the bacterium bag equipped with second class inoculum compost after sterilizing, a test tube first class inoculum connects 10 second class inoculum bags；
(5) condition of culture:
By bacterium bag postvaccinal in step (4), it is positioned over indoor cultivation, room temperature 30 DEG C, relative air humidity natural drying, indoor lucifuge, well-ventilated, after the long purseful of mycelia, under the conditions of room temperature 20 DEG C, it is further cultured for 40d so that it is complete the physiological process of mycelia after-ripening；
(6) go out sesame to manage:
When outdoor temperature reaches more than 5 DEG C, the bacterium bag cultivated in step (5) is buckled canopy and shelters from heat or light, and is removed by the tampon of bacterium bag mouth, put ground, highly 15 layers, to pendulum at the bottom of bag, line space 50cm, ceiling installs equipment of sprinkler irrigation, and initial stage temperature of shed keeps 25 DEG C, and humidity keeps 85%, after the former base of Ganoderma tsugae is formed, temperature of shed keeps 30 DEG C, and humidity keeps 95%, 42d can gather dry in the sun；
(7) gather:
When cap edge becomes bronzing, can gather, remove stem root impurity and be placed on gauze, daylight natural drying.
Embodiment 3:
Culture material formula:
Ganoderma tsugae first class inoculum compost is by Rhizoma Solani tuber osi 200 parts, glucose 15 parts, agar powder 20 parts, potassium dihydrogen phosphate 3 parts, 1.5 parts of magnesium sulfate, peptone 1 part, 1000 parts of weight proportions of water；
Ganoderma tsugae second class inoculum compost is made up of broad-leaved wood flour 75%, pinaster wood flour 6.5%, Testa Tritici 15%, Gypsum Fibrosum 1.5%, Semen Glycines powder 2%.
A kind of method of Ganoderma tsugae compost cultivation Ganoderma tsugae being suitable to the Northeast's cultivation, comprises the following steps,
(1) strain separating:
Select wild Ganoderma tsugae sporophore, in workbench, with 75% alcohol wipe Ganoderma tsugae sporophore surface, the tissue in picking stem and cap joint portion, access rapidly in inclined-plane, put in 30 DEG C of constant incubators and cultivate, routine observation, by microscopy, selects the strain grown fine, become first class inoculum standby, eliminate and pollute strain；
(2) first class inoculum culture medium makes and first class inoculum inoculation；
First class inoculum culture medium makes and chooses Rhizoma Solani tuber osi 200 parts, glucose 15 parts, agar powder 20 parts, potassium dihydrogen phosphate 3 parts, 1.5 parts of magnesium sulfate, peptone 1 part, 1000 parts of weight proportions of water, Rhizoma Solani tuber osi is through peeling, stripping and slicing, liquor, sieve the mixing that adds water, access first class inoculum after tubulature, sterilizing, cooling, cultivate good rear low-temperature preservation standby；
(3) making of second class inoculum culture medium:
Weigh broad-leaved wood flour 75%, pinaster wood flour 6.5%, Testa Tritici 15%, Gypsum Fibrosum 1.5%, Semen Glycines powder 2% by mass percentage, sieve the mixing that adds water, make water content 62%, mix thoroughly, load in the Polythene Bag of 16.5cm × 33cm specification, every packed culture medium wet feed 1.3k part, uses the sealing of bacterium rod to put upside down, forms the bacterium bag equipped with second class inoculum compost；
(4) sterilizing and inoculation:
By the bacterium bag equipped with second class inoculum culture medium produced in step (3), use normal pressure moist heat sterilization, sterilizing 8h at 100 DEG C, standby, being accessed by first class inoculum produced in step (2) in the bacterium bag equipped with second class inoculum compost after sterilizing, a test tube first class inoculum connects 10 second class inoculum bags；
(5) condition of culture:
By bacterium bag postvaccinal in step (4), it is positioned over indoor cultivation, room temperature 27 DEG C, relative air humidity natural drying, indoor lucifuge, well-ventilated, after the long purseful of mycelia, under the conditions of room temperature 18 DEG C, it is further cultured for 32d so that it is complete the physiological process of mycelia after-ripening.
(6) go out sesame to manage:
When outdoor temperature reaches more than 5 DEG C, the bacterium bag cultivated in step (5) is buckled canopy and shelters from heat or light, and is removed by the tampon of bacterium bag mouth, put ground, highly 14 layers, to pendulum at the bottom of bag, line space 50cm, ceiling installs equipment of sprinkler irrigation, and initial stage temperature of shed keeps 23 DEG C, and humidity keeps 85%, after the former base of Ganoderma tsugae is formed, temperature of shed keeps 27 DEG C, and humidity keeps 90%, 38d can gather dry in the sun；
(7) gather:
When cap edge becomes bronzing, can gather, remove stem root impurity and be placed on gauze, daylight natural drying.
Embodiment 4:
Culture material formula:
Ganoderma tsugae first class inoculum compost is made up of Rhizoma Solani tuber osi 150 parts, glucose 10 parts, agar powder 14 parts, potassium dihydrogen phosphate 2 parts, 1 part of magnesium sulfate, peptone 0.5 part, water 1000ml；
Ganoderma tsugae second class inoculum compost is made up of broad-leaved wood flour 71%, pinaster wood flour 7%, Testa Tritici 20%, Gypsum Fibrosum 1%, Semen Glycines powder 1%.
A kind of method of Ganoderma tsugae compost cultivation Ganoderma tsugae being suitable to the Northeast's cultivation, comprises the following steps,
(1) strain separating:
Select wild Ganoderma tsugae sporophore, in workbench, with 70% alcohol wipe Ganoderma tsugae sporophore surface, the tissue in picking stem and cap joint portion, access rapidly in inclined-plane, put in 15 DEG C of constant incubators and cultivate, routine observation, by microscopy, selects the strain grown fine, become first class inoculum standby, eliminate and pollute strain；
(2) first class inoculum culture medium makes and first class inoculum inoculation；
First class inoculum culture medium makes and chooses Rhizoma Solani tuber osi 150 parts, glucose 25 parts, agar powder 14 parts, potassium dihydrogen phosphate 2 parts, 1 part of magnesium sulfate, peptone 0.5 part, 1000 parts of weight proportions of water, Rhizoma Solani tuber osi is through peeling, stripping and slicing, liquor, sieve the mixing that adds water, access first class inoculum after tubulature, sterilizing, cooling, cultivate good rear low-temperature preservation standby；
(3) making of second class inoculum culture medium:
Weigh broad-leaved wood flour 71%, pinaster wood flour 7%, Testa Tritici 20%, Gypsum Fibrosum 1%, Semen Glycines powder 1% by mass percentage, sieve the mixing that adds water, make water content 60%, mix thoroughly, load in the Polythene Bag of 16.5cm × 33cm specification, every packed culture medium wet feed 1.2k part, uses the sealing of bacterium rod to put upside down, forms the bacterium bag equipped with second class inoculum compost；
(4) condition of culture:
Select the forest land that suitable Ganoderma tsugae grows, partly become thoroughly decomposed in utilizing forest land, and there is no the stump that other miscellaneous bacterias infect, in the back of stump, 1 centimeters high with ground, it is perpendicular to stump boring, access cultured Ganoderma tsugae second class inoculum, after the bacterium bag of second class inoculum being taken off plastic bag simultaneously, docile stump embedment underground, make Ganoderma tsugae mycelia invade stump, grow under natural environment.
Embodiment 4 have employed the cultural method of ecosystem, need not build booth, it is not take up the interior space, the growth that stump is Ganoderma is utilized to supply nutrients, it it is the cultural method of complete green, ecological, environmental protective, extend the growth time of Ganoderma tsugae simultaneously, expand the increment of Ganoderma tsugae, make the nutritional labeling of Ganoderma tsugae be effectively improved.
The invention have the advantages that
(1) formula of culture medium is made up of Rhizoma Solani tuber osi, glucose, agar powder, potassium dihydrogen phosphate, magnesium sulfate, peptone, water first class inoculum compost, containing the required inorganic salt of Ganoderma tsugae growth in this formula, including potassium, magnesium, phosphorus.
(2) Ganoderma tsugae second class inoculum compost is made up of broad-leaved wood flour, pinaster wood flour, Testa Tritici, Gypsum Fibrosum, Semen Glycines powder.Containing the required carbon source of Ganoderma tsugae growth in this formula, especially Testa Tritici and Gypsum Fibrosum are added in sawdust medium, the height of the Ganoderma yield rate of production.
(3) bag cultivating method advantage: the Ganoderma fresh sesame weight of production is big, yield rate is high；The sesame lid of the Ganoderma produced is big；The Polypropylene Bag low cost used planted by bag；Use Polypropylene Bag convenient in carrying.
(4) the tame first class inoculum of wild species source selection-breeding is utilized, improve the yield rate of Ganoderma tsugae, and enhance Ganoderma tsugae and resist the quality of extraneous poor environment, the nutritive value making the Ganoderma tsugae after growth has obtained significant raising, finally gives the comparatively ideal method of a Ganoderma indoors artificial cultivation.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.All within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (2)
1. the Ganoderma tsugae compost being suitable to the Northeast's cultivation, it is characterised in that: include Song Shan
Ganoderma first class inoculum compost and Ganoderma tsugae second class inoculum compost, described Ganoderma tsugae first class inoculum is trained
Nutriment is Rhizoma Solani tuber osi 150～250 parts, glucose 10～25 parts, agar powder 14～25 parts, biphosphate
The parts by weight of potassium 2～4 parts, magnesium sulfate 1～2 parts, peptone 0.5～1.5 parts, 1000 parts of water are joined
Put；The compost of described Ganoderma tsugae second class inoculum by broad-leaved wood flour 70～80%, pinaster wood flour 5～10%,
Testa Tritici 13～20%, Gypsum Fibrosum 1～2%, Semen Glycines powder 1～3% composition.
2. one kind uses the Ganoderma tsugae compost being suitable to the Northeast's cultivation as claimed in claim 1 to plant
The method of training Ganoderma tsugae, it is characterised in that comprise the following steps,
(1) strain separating:
Select wild Ganoderma tsugae sporophore, in workbench, with 70～75% alcohol wipe Ganoderma tsugae
Tissue in sporophore surface, picking stem and cap joint portion, accesses in inclined-plane rapidly, put into 15～
30 DEG C of constant incubators are cultivated, routine observation, by microscopy, select the strain grown fine, become
First class inoculum is standby, eliminates and pollutes strain；
(2) first class inoculum culture medium makes and first class inoculum inoculation；
First class inoculum culture medium makes and chooses Rhizoma Solani tuber osi 150～250 parts, glucose 10～25 parts, agar
Powder 14～25 parts, potassium dihydrogen phosphate 2～4 parts, magnesium sulfate 1～2 parts, peptone 0.5～1.5 parts,
The parts by weight that water is 1000 parts configure, and Rhizoma Solani tuber osi is through peeling, stripping and slicing, liquor, and sieving, it is mixed to add water
Close, after tubulature, sterilizing, cooling, access first class inoculum, cultivate good rear low-temperature preservation standby；
(3) making of second class inoculum culture medium:
Weigh by mass percentage broad-leaved wood flour 70～80%, pinaster wood flour 5～10%, Testa Tritici 13～20%,
Gypsum Fibrosum 1～2%, Semen Glycines powder 1～3%, the mixing that adds water of sieving, make water content 60%～65%, mix thoroughly, load
In the Polythene Bag of 16.5cm × 33cm specification, every packed culture medium wet feed 1.2～1.3k part, use bacterium
Rod sealing is put upside down, forms the bacterium bag equipped with second class inoculum compost；
(4) sterilizing and inoculation:
The bacterium bag equipped with second class inoculum culture medium produced in step (3) is used normal pressure moist heat sterilization,
At 100 DEG C, sterilizing 8h, standby, first class inoculum produced in step (2) is accessed after sterilizing equipped with
In the bacterium bag of second class inoculum compost, a test tube first class inoculum connects 6～10 second class inoculum bags；
(5) condition of culture:
By bacterium bag postvaccinal in step (4), it is positioned over indoor cultivation, room temperature 15～30 DEG C, air
Relative humidity natural drying, indoor lucifuge, well-ventilated, after the long purseful of mycelia, in room temperature 15～20
30～40d it are further cultured for so that it is complete the physiological process of mycelia after-ripening under the conditions of DEG C；
(6) go out sesame to manage:
When outdoor temperature reaches more than 5 DEG C, the bacterium bag cultivated in step (5) is buckled canopy and shelters from heat or light, by bacterium bag
The tampon of mouth removes, and puts ground, highly 12～15 layers, to pendulum at the bottom of bag, and line space 50cm, ceiling
Installing equipment of sprinkler irrigation, initial stage temperature of shed keeps 15～25 DEG C, and humidity keeps 70%～85%, works as Song Shanling
After the former base of sesame is formed, temperature of shed keeps 25～30 DEG C, and humidity keeps 85%～95%, and 35～42d can adopt
Receive dry in the sun；
(7) gather:
When cap edge becomes bronzing, can gather, remove stem root impurity and be placed on gauze, day
Light natural drying.
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| CN112088718A (en) * | 2019-06-17 | 2020-12-18 | 米林县米林镇红太阳科技示范家庭农场 | Preparation method of milin ganoderma lucidum strain |
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| CN105315055A (en) * | 2014-07-22 | 2016-02-10 | 王健 | Pine-tree wood chip medium suitable for growth of domestic fungi |
| CN104737819A (en) * | 2015-04-14 | 2015-07-01 | 马凤 | Method for cultivating ganoderma tsugae by taking off a half of bag |
| CN104798599A (en) * | 2015-04-22 | 2015-07-29 | 吉林农业大学 | Method for planting ganoderma tsugae by imitating wild growth in forest land |
| CN106305135A (en) * | 2016-08-17 | 2017-01-11 | 吴庆旺 | Coriolus and lucid ganoderma edible mushroom and preparation method thereof |
| CN106489542B (en) * | 2016-12-21 | 2019-03-29 | 黑龙江省农业科学院牡丹江分院 | A kind of Ganoderma tsugae quick-growing cultivation method |
| CN109874598A (en) * | 2019-04-18 | 2019-06-14 | 中国林业科学研究院华北林业实验中心 | A kind of standardized planting method of red sesame |
| CN111972210A (en) * | 2020-08-31 | 2020-11-24 | 福建省农业科学院农业生态研究所 | Ganoderma lucidum cultivation method for efficiently converting resveratrol in grape vines |
| CN115191291A (en) * | 2022-08-06 | 2022-10-18 | 海南省林业科学研究院(海南省红树林研究院) | Domestication and cultivation method for auricularia auricula |
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| CN102960184A (en) * | 2012-11-22 | 2013-03-13 | 兴隆县庆旺富硒食用菌农民专业合作社 | Edible fungus strain rich in organic selenium, preparation method therefore, edible fungus rich in organic selenium and culturing method thereof |
| CN103314771A (en) * | 2013-04-18 | 2013-09-25 | 广东省微生物研究所 | New strain of ganoderma tsugae Murr. and cultivation method of strain |
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| CN101755615A (en) * | 2009-12-31 | 2010-06-30 | 张寿禄 | Cultivation method of high trace element edible and medical fungi |
| CN102870595A (en) * | 2012-09-13 | 2013-01-16 | 广东省微生物研究所 | Artificial cultivation method capable of realizing highland cultivation of wild ganoderma tsugae murr |
| CN102960184A (en) * | 2012-11-22 | 2013-03-13 | 兴隆县庆旺富硒食用菌农民专业合作社 | Edible fungus strain rich in organic selenium, preparation method therefore, edible fungus rich in organic selenium and culturing method thereof |
| CN103314771A (en) * | 2013-04-18 | 2013-09-25 | 广东省微生物研究所 | New strain of ganoderma tsugae Murr. and cultivation method of strain |
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| CN112088718A (en) * | 2019-06-17 | 2020-12-18 | 米林县米林镇红太阳科技示范家庭农场 | Preparation method of milin ganoderma lucidum strain |
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