CN105999405A - Absorbable fundal mesh composite and preparation method thereof - Google Patents
Absorbable fundal mesh composite and preparation method thereof Download PDFInfo
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- CN105999405A CN105999405A CN201610338031.7A CN201610338031A CN105999405A CN 105999405 A CN105999405 A CN 105999405A CN 201610338031 A CN201610338031 A CN 201610338031A CN 105999405 A CN105999405 A CN 105999405A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/28—Materials for coating prostheses
- A61L27/34—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/0004—Closure means for urethra or rectum, i.e. anti-incontinence devices or support slings against pelvic prolapse
- A61F2/0031—Closure means for urethra or rectum, i.e. anti-incontinence devices or support slings against pelvic prolapse for constricting the lumen; Support slings for the urethra
- A61F2/005—Closure means for urethra or rectum, i.e. anti-incontinence devices or support slings against pelvic prolapse for constricting the lumen; Support slings for the urethra with pressure applied to urethra by an element placed in the vagina
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
- A61L2300/604—Biodegradation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2420/00—Materials or methods for coatings medical devices
- A61L2420/04—Coatings containing a composite material such as inorganic/organic, i.e. material comprising different phases
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- Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Public Health (AREA)
- Life Sciences & Earth Sciences (AREA)
- Transplantation (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Biomedical Technology (AREA)
- Cardiology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses an absorbable fundal mesh composite and a preparation method thereof. The composite is composed of a biofilm matrix and a coating layer, and the coating layer is evenly distributed on the surface of the biofilm matrix; the biofilm matrix is formed by defatting and decellularizing natural biofilm; the coating layer is made with biodegradable high-molecular polymers; the biofilm matrix is also subjected to crosslinking treatment. The biofilm matrix is used as a matrix material, the biofilm matrix is subjected to crosslinking treatment, the coating layer is made from the biodegradable high-molecular polymers, and the prepared fundal mesh composite has good mechanical properties and good biocompatibility, can be absorbed and degraded by living organisms, and serves as a novel composite for operation of pelvic organ prolapse.
Description
Technical field
The invention belongs to Material Field, be specifically related to sticking patch composite and preparation method thereof at the bottom of a kind of absorbable palace.
Background technology
Pelvic organ prolapse and stress incontinence be have a strong impact in, the common disease of elderly woman quality of life.Along with life
The raising day by day of level, this type of disease is subject to people's attention further, and then Pelvic pain syndrome surgery becomes an emerging subject.
The modus operandi of conventional therapy pelvic organ prolapse is female hysterectomy and the repairing of vagina front and back walls, but this therapeutic modality is postoperative
Relapse rate and again operability are all more than 30%.Further investigated has been carried out, wherein for reducing recurrence after operation rate this area research worker
Application including sticking patch.
The feature repaired according to pelvic floor tissue, preferable Pelvic pain syndrome material should have characteristics that 1. minimum foreign body reaction；
The most flexible flexible；The most easily sew up；4. preferable histocompatibility；5. collagen fiber are allowed to grow into；6. tissue can be made
Permanent reparation；7. there is preferable tension force；8. can infection；9. less complication is caused.And currently patching material has
The most several: the nonabsorable sticking patch 1. synthesized；2. the absorbable sticking patch synthesized；The most autologous or allotransplant.In general,
Have without mucosa abrasion as the autologous tissue of reconstruction biomaterials, there is not pathophoresis risk and the extremely low advantage of infection rate, but can
The complication relevant to harvesting of fascia can be there is, such as the collection sexual trauma etc. of lower limb or abdominal part.It is steady that synthetic material then has character
Fixed, firm, apply convenient feature, but there may be infection, repel and the complication such as mucosa abrasion.Therefore, exploitation has
Preferably the patching material of biocompatibility is still the work of a great meaning.
Summary of the invention
In view of this, an object of the present invention is to provide sticking patch composite at the bottom of the absorbable palace of one, and this composite is with de-
The biofilm matrix of cell be skeleton, high molecular polymer or high-molecular copolymer be film, degraded slowly, possesses good life
The thing compatibility and mechanical property；It addition, present invention also offers the preparation method of this composite.
The technical scheme that the present invention takes is as follows:
1, sticking patch composite at the bottom of absorbable palace, is made up of biofilm matrix and coating；Described coating uniform is distributed in biomembrane base
Matter surface, is made up of Biodegradable high-molecular polymer；Described biofilm matrix is made up of after defat takes off cell biomembrane,
The crosslinked process of biofilm matrix is to strengthen mechanical performance.
Preferably, described biomembrane is the pericardium of animal, submucous layer of small intestine or mucous membrane of urinary bladder.
Preferably, described biofilm matrix processes through chemical crosslinking.
Preferably, described chemical crosslinking processes and uses bridging agent, and described bridging agent is 1-(3-dimethylamino-propyl)-3-ethyl carbon two
Inferior amine salt hydrochlorate/N-hydroxy-succinamide mixed solution, isocyanates, glutaraldehyde, formaldehyde, paraformaldehyde or genipin.
Preferably, described Biodegradable high-molecular polymer is pla-pcl, polylactic acid, polyglycolic acid, gathers dioxocyclohex
Ketone, poly (glycolide-lactide) or their modified polymer；
Preferably, modification by copolymerization it is modified as described in.
2, the preparation method of sticking patch composite at the bottom of absorbable palace, comprises the steps:
1) biomembrane is taken；
2) defat: by step 1) obtain biomembrane be placed in soak degreasing 12h in the methanol-chloroform mixed liquor of volume ratio 1:1, go
Ionized water rinses 3 times；
3) de-cell: by step 2) material after defat is placed in 0.9% normal saline containing pancreatin and EDTA, and 4 DEG C are shaken
Swinging digestion 48h, ionized water cleans 3 times repeatedly；The weight/mass percentage composition of described pancreatin and EDTA is 0.05%；
4) biofilm matrix is carried out crosslinking Treatment；
5) lyophilizing: the biofilm matrix cross-linked is placed in-20 DEG C of freezing shaping 12h, then-95 DEG C of negative pressure lyophilizing, keeps negative
It is pressed in below 0.2 hundred handkerchiefs 48h；
6) coating: with solvent by Biodegradable high-molecular polymer dissolve, by step 5) process after biofilm matrix dipping
In Biodegradable high-molecular polymer after dissolving, 3 days final vacuums of natural drying are dried 1 day, deionized water rinsing,
After be vacuum dried again 1 day and can obtain sticking patch composite at the bottom of absorbable palace.
Preferably, described step 4) described in crosslinking Treatment for use bridging agent be chemically crosslinked.
Preferably, described step 6) described in solvent be hexafluoroisopropanol, chloroform, TEG, carbon tetrachloride or acetic acid second
Ester.
It should be noted that biomembranous acquisition includes biomembranous stripping, goes the operations such as fat, obtaining step is this area
Conventional mode of operation.
The crosslinking Treatment of biofilm matrix includes the crosslinking Treatment mode that this areas such as physical crosslinking, chemical crosslinking are conventional, it is therefore an objective to
For strengthening the mechanical performance of biofilm matrix；Physical crosslinking includes utilizing light, heat radiation etc. to carry out crosslinked polymer；Chemical crosslinking
Including using bridging agent infusion process or gas bridge formation method etc..
It is conventional method that solvent dissolves the method for Biodegradable high-molecular polymer, as a example by polycaprolactone, and can be by it with poly-
Ether F127 dissolves in the mode of 90-160 DEG C of mixing.
Described Biodegradable high-molecular polymer can be polycaprolactone (PCL), polylactic acid (PLA, containing PLLA), gather
Any one in glycolic (PGA), PPDO (PPDO), poly (glycolide-lactide) (PLGA), it is also possible to be
One in its copolymer, such as PPDO-PLLA, PLLA-PCL, PLLA-PGA etc..
Described modification can be the modification mode such as blending and modifying, filling-modified, chemical modification, as long as modified material can be real
Existing technical scheme of the present invention.
The beneficial effects of the present invention is: the present invention take biofilm matrix as host material, and biofilm matrix through friendship
Connection processes, and coating then uses biodegradable high molecular polymer, and sticking patch composite at the bottom of the palace that preparation obtains not only has good
Good mechanical performance, also has good biocompatibility, it is possible to absorbed degraded by organism, carries for pelvic organ prolapse operation
Supply a kind of new composite.
Accompanying drawing explanation
In order to make the purpose of the present invention, technical scheme and beneficial effect clearer, the present invention provides drawings described below:
Fig. 1 is the cell free clean diaphanous Cor Sus domestica peplos of non-defat after fat strikes off；
Fig. 2 is the cell free schematic diagram of Cor Sus domestica peplos；
Fig. 3 is the Cor Sus domestica peplos substrate after defat takes off cell；
Fig. 4 is the Cor Sus domestica peplos substrate after frozen dried；
Fig. 5 be embodiment 1 defat take off the Cor Sus domestica peplos substrate after cell and chemical crosslinking process after the scanning of Cor Sus domestica peplos substrate
Electronic Speculum figure；Wherein, A figure and B figure are respectively defat and take off the Cor Sus domestica peplos substrate 400 times after cell and the scanning electron microscope of 2000 times
Figure, C figure and D figure are respectively defat and take off the Cor Sus domestica peplos substrate after cell after EDC/NHS mixed liquor cross-links 400 times and 2000
Scanning electron microscope (SEM) photograph again；
Fig. 6 is sticking patch composite at the bottom of the absorbable palace that embodiment 1 obtains；
Fig. 7 is scanning electron microscope (SEM) photograph when being amplified to 1000 times of the sticking patch composite at the bottom of palace；
Fig. 8 is scanning electron microscope (SEM) photograph when being amplified to 2000 times of the sticking patch composite at the bottom of palace.
Detailed description of the invention
Below the preferred embodiments of the present invention are described in detail.The experimental technique of unreceipted actual conditions in embodiment is logical
Often according to normal condition or according to the condition proposed by manufacturer.
Embodiment 1
Sticking patch composite at the bottom of described absorbable palace is made up of biofilm matrix and coating, and described coating uniform is distributed in biomembrane base
Matter surface；Described biofilm matrix is made up of after defat takes off cell the pericardium of pig；Described coating is by Biodegradable high-molecular
Polymer forms；Described biofilm matrix carries out chemical crosslinking process through using bridging agent.Sticking patch composite wood at the bottom of this absorbable palace
Preparation method for material comprises the steps:
1) take biomembrane: the heart of pig or cattle is separated with pericardium by mechanical means, and by the fatty scraped clean on pericardium, repeatedly rush
Wash the pericardium of clean near-transparent shape is standby；
2) defat: the Cor Sus domestica peplos of acquirement is placed in soak degreasing 12h in the methanol of volume ratio 1:1 and chloroform mixed liquor, deionized water
Rinse 3 times, amount to 24h；
3) de-cell: the material after defat is placed in 4 DEG C of vibration digestion 48h in 0.9% normal saline containing pancreatin and EDTA, from
Sub-water cleans 3 times repeatedly；The weight/mass percentage composition of described pancreatin and EDTA is 0.05%；
4) chemical crosslinking: defat is taken off the Cor Sus domestica coated fertilizer after cell and is placed in 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide hydrochloride
In salt (EDC)/N-hydroxy-succinamide (NHS) mixed solution, biomembrane and EDC/NHS mixed solution are with 8:1
(W/V) ratio mixing, in 20 DEG C, 400r/min concussion crosslinking 12h；Then concussion is cleaned 5 times, each 10min；
The compound method of EDC with NHS mixed solution is: EDC and NHS is dissolved in the MES buffering that pH value is 5.5 molten
In liquid, EDC and NHS concentration is respectively 0.1mol/L and 0.05mol/L；
5) lyophilizing (is anhydrated and unnecessary cross-linking agent): the biofilm matrix cross-linked be placed in culture dish, shapes at-20 DEG C of refrigerator freezings
12h, puts in low-temperature freeze-drying machine ,-95 DEG C of negative pressure lyophilizing, keeps negative pressure at 0.2hPa (hundred handkerchiefs) following 48h；
6) coating: the polycaprolactone (PCL) that 5g molecular weight is 20000 is dissolved in 20ml chloroform, and 40 DEG C of shaking tables make it be completely dissolved；
By step 5) in the biofilm matrix of lyophilizing be placed in glass container, and pour the PCL coating solution prepared into and make biology
Membrane matrix thorough impregnation, air drying 3 days, then be vacuum dried 1 day, then material is taken out from container, deionization
Water rinses repeatedly, is vacuum dried 1 day the most again, thus prepare complete thickness be 2mm, length x width=50 × 50mm
Absorbable palace at the bottom of sticking patch composite, wherein the thickness of native biomatrix film (Cor Sus domestica peplos) is 0.43mm, length ×
Width is 50 × 50mm.
Embodiment 2
Sticking patch composite at the bottom of described absorbable palace is made up of biofilm matrix and coating, and described coating uniform is distributed in biomembrane base
Matter surface；Described biofilm matrix is made up of after defat takes off cell the submucous layer of small intestine of pig；Described coating is by biodegradable
High molecular polymer forms；Described biofilm matrix carries out chemical crosslinking process through using bridging agent.Sticking patch at the bottom of this absorbable palace
Composite material and preparation method thereof comprises the steps:
1) biomembrane is taken: mechanical means removes pig small intestine placenta percreta, placenta percreta, muscle layer, obtains submucous layer of small intestine, repeatedly rinses
Standby；
2) defat: the trees-Osima jacoti, Osima excavata of acquirement is placed in soak degreasing in the methanol of volume ratio 1:1 and chloroform mixed solution
12h, deionized water rinses 3 times；
3) de-cell: the trees-Osima jacoti, Osima excavata material after above-mentioned defat is placed in the 0.9% physiology salt containing pancreatin and EDTA
4 DEG C of vibration digestion 48h in water, ionized water cleans 3 times repeatedly；The weight/mass percentage composition of described pancreatin and EDTA is 0.05%；
The normal saline of described 0.9% is the sodium chloride solution of mass fraction 0.9%；
4) chemical crosslinking biofilm matrix: the method using gas to build bridge carries out crosslinking Treatment, and gas bridge formation condition is mass fraction
Under 25% glutaraldehyde saturated vapor, 37 DEG C process 4 hours；Deionized water rinses 3 times；
5) lyophilizing (is anhydrated and unnecessary cross-linking agent): the biofilm matrix cross-linked be placed in culture dish, at-20 DEG C of refrigerator freezings
Shape 12h, put in low-temperature freeze-drying machine (SCANVAC board CoolSafe) ,-95 DEG C of negative pressure lyophilizing, keep negative pressure to exist
0.2hPa (hundred handkerchiefs) following 48h；
6) coating: by step 5) trees-Osima jacoti, Osima excavata of lyophilizing is placed in container, and pour the PGA-PPDO prepared into and be coated with
Layer solution impregnation, air drying 3 days, then be vacuum dried 1 day, then material being taken out from container, deionized water is repeatedly
Rinse, be vacuum dried the most again 1 day, prepare sticking patch composite at the bottom of absorbable palace；The size of this patching material is thick 1.5mm,
Long × wide=20mm × 5mm, wherein step 5) lyophilizing after material thickness be 1.17mm, length and width is equal to patch size.Should
Composite machinery is functional, and biocompatibility is good, and can be with biodegradation.Described PGA-PPDO coating solution is by following
Prepared by method: PGA and PPDO, by the mass ratio hexafluoroisopropanol mixed dissolution of 2:3, is heated to 60 DEG C of prepared PGA-PPDO.
Described step 6) in coating material can also is that polylactic acid (PLA, containing PLLA), polyglycolic acid (PGA), poly-
Any one in Lanthanum Isopropoxide (PPDO), poly (glycolide-lactide) (PLGA), it is also possible to be the one in its copolymer,
Such as PPDO-PLLA, PLLA-PCL, PLLA-PGA etc..Coating material is dissolved in solvent and can use fusion method, with
F127, as catalyst, is heated to 90-160 DEG C of mixing, treat solution be down to less than 37 DEG C stand-by.As a example by PCL, can be by 12
PCL and 0.6wt%F127 of wt% mixing at 90-160 DEG C, is subsequently poured in mould, and THICKNESS CONTROL, at 0.5mm, immerses
2h molding in water.
Fig. 1 is the cell free clean diaphanous Cor Sus domestica peplos of non-defat after fat strikes off；Fig. 2 is that Cor Sus domestica peplos is cell free to be shown
Being intended to, the liquid soaking Cor Sus domestica peplos in bottle is 0.9% normal saline containing pancreatin and EDTA；Fig. 3 is for take off cell through defat
After Cor Sus domestica peplos substrate；Fig. 4 is the Cor Sus domestica peplos substrate after frozen dried；Fig. 5 is after embodiment 1 defat takes off cell
Cor Sus domestica peplos substrate and chemical crosslinking process after the scanning electron microscope (SEM) photograph of Cor Sus domestica peplos substrate, as seen from the figure, through chemical crosslinking
Cor Sus domestica peplos substrate crosslinking group collagen fiber structure after process relatively the former more closely in order, in being connected with each other, arranged in parallel many
Hole pattern shape；Fig. 6 is sticking patch composite at the bottom of the absorbable palace that embodiment 1 obtains, and Fig. 7 and 8 divides for sticking patch composite at the bottom of palace
The not scanning electron microscope (SEM) photograph under 1000 and 2000 times, as seen from the figure, sticking patch composite structure at the bottom of palace prepared by the present invention is more
Fine and close.
Example 1 takes off the Cor Sus domestica peplos three parts after cell with a batch of defat, carries out Mechanics Performance Testing (GB/T
1040.3-2006), the results are shown in Table 1.
Table 1 defat takes off the Cor Sus domestica peplos mechanical experimental results after cell
Example 1, with the Cor Sus domestica peplos three parts after a batch of EDC/NHS crosslinking, carries out Mechanics Performance Testing, and result is shown in
Table 2.
Cor Sus domestica peplos mechanical experimental results after table 2EDC/NHS crosslinking
Example 1, with sticking patch composite sample three parts at the bottom of a batch of palace, carries out Mechanics Performance Testing, the results are shown in Table 3.
Sticking patch composite materials property test result at the bottom of table 3 palace
From table 1-3, at the bottom of the final palace obtained, sticking patch composite is due to through crosslinking Treatment and coating of high molecular polymer
Processing, its mechanical property is greatly enhanced.
Example 1, with the Cor Sus domestica peplos three parts after a batch of EDC/NHS crosslinking, carries out puncturing experiment test, and result is shown in
Table 4.
Cor Sus domestica peplos after table 4EDC/NHS crosslinking punctures experimental result
Example 1, with three parts of sticking patch composite at the bottom of a batch of palace, carries out puncturing experiment test, the results are shown in Table 5.
Sticking patch composite at the bottom of table 5 palace punctures experimental result
Cor Sus domestica coated fertilizer is a kind of natural biologic material, inclined according to test results indivedual in the difference of its thickness, table 4 and table 5
Difference is slightly larger, and owing to the framing structure of sticking patch composite at the bottom of palace is Cor Sus domestica peplos substrate, this result also shows Cor Sus domestica peplos from side
Substrate plays an important role in the mechanical property of sticking patch composite at the bottom of palace.The puncture experimental result explanation of table 4,5: sticking patch at the bottom of palace
Composite its puncture mechanics bearing capacity after Biodegradable high-molecular coating is greatly improved compared to native biomatrix material,
And remaining on the good elongation ability of native biomatrix material, the tissue being suitably applied prolapse surgery at the bottom of palace is built admittedly.
Finally illustrating, preferred embodiment above is only in order to illustrate technical scheme and unrestricted, although by above-mentioned
The present invention is described in detail by preferred embodiment, it is to be understood by those skilled in the art that can in form and
In details, it is made various change, without departing from claims of the present invention limited range.
Claims (9)
1. it can absorb sticking patch composite at the bottom of palace, it is characterised in that be made up of biofilm matrix and coating；Described coating uniform divides
It is distributed in biofilm matrix surface, is made up of Biodegradable high-molecular polymer；Described biofilm matrix is taken off through defat by biomembrane
Constituting after cell, the crosslinked process of biofilm matrix is to strengthen mechanical performance.
Sticking patch composite at the bottom of absorbable palace the most according to claim 1, it is characterised in that described biomembrane is animal
Pericardium, submucous layer of small intestine or mucous membrane of urinary bladder.
Sticking patch composite at the bottom of absorbable palace the most according to claim 1, it is characterised in that described biofilm matrix is through changing
Learn crosslinking Treatment.
Sticking patch composite at the bottom of absorbable palace the most according to claim 3, it is characterised in that described chemical crosslinking processes and adopts
With bridging agent, described bridging agent is 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride/N-hydroxy-succinamide mixing
Solution, isocyanates, glutaraldehyde, formaldehyde, paraformaldehyde or genipin.
Sticking patch composite at the bottom of absorbable palace the most according to claim 1, it is characterised in that described biodegradable high score
Sub-polymer is pla-pcl, polylactic acid, polyglycolic acid, PPDO, poly (glycolide-lactide) or they are modified
Polymer.
Sticking patch composite at the bottom of absorbable palace the most according to claim 5, it is characterised in that described in be modified as modification by copolymerization.
7. the preparation method of sticking patch composite at the bottom of absorbable palace described in any one of claim 1～6, it is characterised in that include as
Lower step:
1) biomembrane is taken；
2) defat: by step 1) obtain biomembrane be placed in soak degreasing 12h in the methanol-chloroform mixed liquor of volume ratio 1:1, go
Ionized water rinses 3 times；
3) de-cell: by step 2) material after defat is placed in 0.9% normal saline containing pancreatin and EDTA, and 4 DEG C are shaken
Swinging digestion 48h, ionized water cleans 3 times repeatedly；The weight/mass percentage composition of described pancreatin and EDTA is 0.05%；
4) biofilm matrix is carried out crosslinking Treatment；
5) lyophilizing: the biofilm matrix cross-linked is placed in-20 DEG C of freezing shaping 12h, then-95 DEG C of negative pressure lyophilizing, keeps negative
It is pressed in below 0.2 hundred handkerchiefs 48h；
6) coating: with solvent by Biodegradable high-molecular polymer dissolve, by step 5) process after biofilm matrix dipping
In Biodegradable high-molecular polymer after dissolving, 3 days final vacuums of natural drying are dried 1 day, deionized water rinsing,
After be vacuum dried again 1 day and can obtain sticking patch composite at the bottom of absorbable palace.
It can absorb the preparation method of sticking patch composite at the bottom of palace the most according to claim 7, it is characterised in that described step 4)
Described in crosslinking Treatment for use bridging agent be chemically crosslinked.
It can absorb the preparation method of sticking patch composite at the bottom of palace the most according to claim 7, it is characterised in that described step 6)
Described in solvent be hexafluoroisopropanol, chloroform, TEG, carbon tetrachloride or ethyl acetate.
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| CN201610338031.7A CN105999405A (en) | 2016-05-19 | 2016-05-19 | Absorbable fundal mesh composite and preparation method thereof |
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| CN201610338031.7A CN105999405A (en) | 2016-05-19 | 2016-05-19 | Absorbable fundal mesh composite and preparation method thereof |
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| CN201610338031.7A Pending CN105999405A (en) | 2016-05-19 | 2016-05-19 | Absorbable fundal mesh composite and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107050520A (en) * | 2017-03-03 | 2017-08-18 | 北京博辉瑞进生物科技有限公司 | Compound bio sticking patch and preparation method thereof |
| CN108042856A (en) * | 2018-02-07 | 2018-05-18 | 苏州元禾医疗器械有限公司 | A kind of preparation method of operation biomembrane |
| CN108114327A (en) * | 2018-02-07 | 2018-06-05 | 苏州元禾医疗器械有限公司 | A kind of operation biomembrane |
| WO2019100454A1 (en) * | 2017-11-27 | 2019-05-31 | 大连理工大学 | Decellularized porous scaffold for three-dimensional tumor model, and construction method therefor and applications thereof |
| CN116531567A (en) * | 2023-05-09 | 2023-08-04 | 成都睿科美医疗科技有限公司 | Endometrial repair material |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN108114327A (en) * | 2018-02-07 | 2018-06-05 | 苏州元禾医疗器械有限公司 | A kind of operation biomembrane |
| CN116531567A (en) * | 2023-05-09 | 2023-08-04 | 成都睿科美医疗科技有限公司 | Endometrial repair material |
| CN116531567B (en) * | 2023-05-09 | 2024-02-13 | 成都睿科美医疗科技有限公司 | Endometrial repair material |
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