CN105998995A

CN105998995A - Kidney tonifying preparation

Info

Publication number: CN105998995A
Application number: CN201610306799.6A
Authority: CN
Inventors: 王敬义; 王苑丽; 王思斐
Original assignee: Chengdu Rongzeyuan Food Co Ltd
Current assignee: Chengdu Rongzeyuan Food Co Ltd
Priority date: 2016-05-11
Filing date: 2016-05-11
Publication date: 2016-10-12

Abstract

The invention relates to a kidney tonifying preparation. The kidney tonifying preparation is prepared from, by weight, 14-20 parts of wulin powder, 8-15 parts of vervain extract, 3-6 parts of Chinese caterpillar fungus extract powder, 5-9 parts of lucid ganoderma, 4-6 parts of fruiting bodies of cordyceps military, 10-15 parts of Chinese dates, 5-10 parts of fructus lycii, 12-15 parts of herba cynomorii, 8-12 parts of radix astragali, 2-6 parts of longan aril, 4-13 parts of cortex cinnamomi and 7-11 parts of fructus alpiniae oxyphyllae. The kidney tonifying preparation can improve the immunity of the human body, has effects of kidney tonifying, nourishing and yang tonifying, and has a certain effect of improving prostatitis.

Description

A kind of kidney-tonifying preparation

Technical field

The present invention relates to a kind of kidney-tonifying preparation.

Background technology

Along with the development of society, the living standard of people also steps up, and the most gradually starts to focus on the health of oneself, to supporting The requirement of body is more and more higher.

CN1336427A (2002-2-20) discloses a kind of lucid ganoderma health preserving wine, but the function for tonifying kidney of this wine need to change Enter.

Summary of the invention

It is an object of the invention to provide a kind of kidney-tonifying preparation that can strengthen body immunity.

The above-mentioned technical purpose of the present invention has the technical scheme that

A kind of kidney-tonifying preparation, it includes the raw material of following weight portion: powdered Wuling mycelia in combination 14-20 part, Herba Verbenae 8-15 part, worm Grass extraction powder 3-6 part, Ganoderma 5-9 part, Cordyceps sporophore 4-6 part, Fructus Jujubae 10-15 part, Fructus Lycii 5-10 part, Herba Cynomorii 12-15 part, Radix Astragali 8-12 part, Arillus Longan 2-6 part, Cortex Cinnamomi 4-13 part, Fructus Alpiniae Oxyphyllae 7-11 part.

Powdered Wuling mycelia in combination, for the mycelium of medicinal fungi WULINGJUN of fermenting, has the effect of the kidney invigorating dehumidifying diuresis.Divide through composition Analysis and clinical practice prove, fermentation WULINGJUN filament is basically identical with crow spirit ginseng crude drug.Clearly. Guangxi's " Guanxian county will " has " benefit Kidney qi " record." Sichuan Province's Chinese medicinal herbal " is recorded, and this product slightly sweet flavor is put down, nontoxic, enters the heart, stomach, bladder three warp, " town of dehumidifying Frightened, reinforcing the heart kidney, diuresis, only cardiopalmus, lactogenic, control insomnia, the disease such as spit blood and lose blood puerperal." the kidney invigorating benefit can be strengthened in side The effect of gas, to effect a permanent cure, can strengthen again the effect of dehumidifying diuresis to take stopgap measures.

Herba Verbenae abundant raw materials, easily produces, and extract verbenalin content is high, can effectively realize in preparation treatment prostatitis Application in adenitis medicine, is the innovation on Chinese medicine.

Fructus Alpiniae Oxyphyllae is the dry mature fruit of zingiberaceous plant, between summer, autumn fruit by green reddening time gather.Fructus Alpiniae Oxyphyllae is containing volatilization Oil, the main composition of volatile oil is the sesquiterpenoidss such as eucalyptol, zingiberene, zingiberol, containing abundant dimension life B and dimension life C, and The elements such as the manganese of trace, zinc, potassium, sodium, calcium, magnesium, phosphorus, ferrum, copper.Main product in mountain area, Hainan Island, the Lezhou Peninsula, Guangdong, Guangxi in addition In area.Warming spleen and stopping diarrha take the photograph saliva, warming the kidney controlling nocturnal emission with astringent drugs reducing urination.Have loose bowels for spleen-cold syndrome, cold type of pain in abdomen, the many salivas of mouth, enuresis due to deficiency of the kidney, urine Frequency, nebulousurine of passing out semen.

Cortex Cinnamomi is the dried bark of Lauraceae aiphyllium plant Cortex Cinnamomi Cinnamomum cassia Presl and thick branch skin.Dried bark Person claims the Cortex Cinnamomi heart to go to epidermis, picks up from thick branch or treelet dried bark person claims Cortex Cinnamomi.Main product is in the ground such as Guangdong, Guangxi.Before Great Heat Bark is isolated, peels off after the beginning of autumn, scrape off cork, dry in the shade.It is processed into plurality of specifications, often because stripping the difference at position and quality That sees has CORTEX CINNAMOMI, plate osmanthus, oiled-plate method osmanthus, Gui Tong etc..Cut into slices or grind into powder, raw use.Taste is sweet the most puckery, slight pungent in containing refreshing The fragrance of mouth.Cure mainly: for sexual impotence, cold womb, trusted subordinate's cold type of pain, deficiency and coldness vomiting and diarrhoea, amenorrhea, dysmenorrhea, warming the meridian and promoting blood circulation.

Ganoderma is the medicinal fungus of a kind of preciousness, is always considered tonification class Chinese crude drug, has strengthening by means of tonics, gets rid of illness and prolong The effect in year.Scientific investigations showed that, the pharmaceutical component of Ganoderma is the abundantest, especially contains following three kinds of precious compositions: have Machine germanium, 17 kinds of aminoacid and macromolecule polysaccharide body, wherein, organic germanium can be combined with internal free hydrogen, with antiperspirant, urine Form excrete, thus improve the oxygen content of human body, health invigorating；Having 7 kinds in 17 kinds of aminoacid is that human body must The aminoacid needed；Macromolecule polysaccharide body can body immunity, regulation metabolism balance.Ganoderma can strengthen people Body immunity, has the several functions such as antiinflammatory, analgesia, antibacterial, diuresis, removing toxic substances, clean blood, to relate to cardiovascular and cerebrovascular vessel, The pathological changes of the system such as digestion, nerve, endocrine, breathing all has certain curative effect.

Cordyceps sporophore, is a kind of famous and precious medicinal fungi, belongs to Eumycota Cordyceps with Qinghai-Tibet wild cordyceps Belong to, have similar medicinal and nourishing effects.

Expert is by thinking to the Research on kinds becoming induction Cordyceps sporophore both at home and abroad: edible, the medicine of Cordyceps militaris (L.) Link. Can compare favourably with traditional wild cordyceps with being worth, the content of its cordycepin even above tradition Cordyceps tens times.

Record according to " whole nation Chinese herbal medicine compilation ": " Cordyceps militaris (L.) Link. entity and polypide can be used as medicine as Cordyceps ". Therefore, Cordyceps militaris pharmacological function and clinical effectiveness aspect are substantially coincident with wild cordyceps.Modern medicine study table Bright, Cordyceps militaris does not contain only rich in protein, and (content is 39.37%, is Carnis Sus domestica, beef, mutton protein content 1.8,1.5,1.9 times) and aminoacid, and the trace element containing more than 30 kind of needed by human body.Wherein P(phosphorus) content Be 3.5 times of Cordyceps, Zn(zinc), Cu(copper), the content of three kinds of elements of Fe(ferrum be 28 kinds of tonic meansigma methodss 1.8,2.1 and 8.8 times, the content of Se (selenium) is suitable with the content of the Radix Astragali.So, Cordyceps militaris has hypnosis town Quiet, liver and kidney tonifying, tonifying deficiency, anti-cancer, anticancer, stop blooding, reduce phlegm, the multiple efficacies such as relieving asthma, with Radix Ginseng, Cornu Cervi Pantotrichum be referred to as The big tonic of in Chinese medicine treasure-house three." Gan Pingbao lung, kidney tonifying, benefit marrow, hemostasisization is being had described in<Bencao Congxin> Expectorant " etc. curative effect.Because Cordyceps enters lung kidney two warp, can tonifying the lung cloudy, again can kidney-replenishing, be uniquely to balance simultaneously and adjust The Chinese medicine of joint negative and positive.

Fructus Lycii (formal name used at school: Lycium chinense) is the multi-branched shrub plant of Solanaceae Lycium.All have both at home and abroad point Cloth.Fructus Lycii whole body is precious, and bright Li Shizhen (1518-1593 A.D.) Compendium of Material Medica is recorded: " spring adopts Folium Lycii, name sky essence grass；Summer picks flowers, name Sempervivum tectorum； Qiu Caizi, name Fructus Lycii；Dong Caigen, name Cortex Lycii ".Fruit claims Fructus Lycii, tender leaf to claim Fructus Lycii head, and frequent species is Fructus Lycii, main The medical species wanted is lycium barbarum.Fructus Lycii has reduction blood glucose, lipotropy, and can atherosclerosis.Fructus Lycii (Barbary Wolfberry Fruit) is famous and precious medical material and tonic, and the traditional Chinese medical science has the saying of " medlar health preserving " very early. Compendium of Material Medica is recorded: " Fructus Lycii, kidney-tonifying sperm-generating, nourishing the liver ... improving eyesight is calmed the nerves make us long-lived.

Herba Cynomorii, has another name called Herba Cynomorii, Herba Cynomorii perennial meat parasitism draft.A kind of parasitic plant, wild in desert and Gobi, Subzero 20 DEG C of growths are the most suitable, in place of growth not accumulated snow, do not freeze.There is the kidney invigorating intestine moistening, control the effects such as sexual impotence, hematuria.

The Radix Astragali, has another name called continuous stilbene.Perennial herb is high 50-100 centimetre.Main root is plump, wooden, normal branch, canescence.Stem Uprightly, top multi-branched, there is thin rib, by white pubescence.Perennial herb is high 50-100 centimetre.Originate in Inner Mongol, Shanxi, sweet The ground such as respectful, Heilungkiang.The medicinal of the Radix Astragali has the history of more than 2000 year so far, its have enhancing human body immunity function, protect the liver, profit Urine, defying age, anti-stress, blood pressure lowering and wide antibacterial action.

Kidney-tonifying preparation is as preferably, and described Ganoderma is Ganoderma lucidum (Leyss. Ex Fr.) Karst. or Fructificatio Amaurodermatis Rudae.

Black Ganoderma, Classification system Ganoderma.The pharmaceutical component of Black Ganoderma is the abundantest, and wherein active ingredient can It is divided into ten big classes, (wherein contains seven kinds of needed by human including ganoderan, Ganoderma polypeptide, triterpenes, 16 kinds of aminoacid Aminoacid), protein, steroid, mannitol, coumarin glycosides, alkaloid, organic acid (main containing Fumaric acid), with And trace element Ge, P, Fe, Ca, Mn, Zn, etc..Ganoderma has dual regulation to human body, kind of being cured the disease, and relates to And cardiovascular and cerebrovascular vessel, digestion, nerve, endocrine, breathe, each system such as motion, especially to tumor, hepatic lesions, The preventive and therapeutic effect of insomnia and aging is the most notable.So as one of Ganoderma, energy brain-strengthening, antiinflammatory, diuresis, stomach reinforcing, There is strengthening by means of tonics effect.According to Shennong's Herbal record, " Ganoderma can be controlled deafness, profit joint, protect vital essence refreshing, beneficial, hard muscle Bone ", control neurasthenia, dizzy insomnia, chronic hepatitis, bronchial asthma.Control neurasthenia, dizzy insomnia, Chronic Liver Scorching, bronchial asthma sporophore and mycelium containing protein, base acid, reducing substances, saccharide, coumarin, steroid or Triterpenes etc..Anti-hypoxia and increase coronary flow effect river note Ganoderma row is had to introduce sporophore and mycelium containing protein, base Acid, reducing substances, saccharide, coumarin, steroid or triterpenes etc..There is anti-hypoxia and increase coronary flow effect." god Agriculture book on Chinese herbal medicine " in the middle of record be: cure mainly " deaf ", " profit joint, protect god, benefit vital essence, heavily fortified point muscles and bones, good color.

The kidney-tonifying preparation of the present invention, while improving body immunity, also has the kidney invigorating Yang nourishing-strengthening effect；To improvement Prostatitis has certain effect.

As preferably, described raw material the most also includes free ergosterol powder 0.6-0.9 part, lucid ganoderma juice 0.3- 0.9 part, Sporoderm-broken Ganoderma Lucidum Spore powder extract 0.3-0.7 part, Cornu Cervi Pantotrichum 0.1-0.2 part, Hippocampus 0.5-0.8 part, Testis et penis callorhini 0.05- 0.08 part, Fructus Rubi 1-2 part, bar halberd 0.3-0.5 part, Radix Angelicae Sinensis 0.2-0.4 part.

Ganoderma spore powder after breaking cellular wall is easier to extract active ingredient, the fat of spore powder with crushed sporoderm and water soluble polysaccharide Content improves 38.95% and 20.83% than non-breaking cellular wall respectively, the reducing sugar in spore powder with crushed sporoderm and polypeptide less breaking cellular wall Spore powder is prone to extract, and the active substance content that the sixth of the twelve Earthly Branches obtains significantly improves；What is more important, spore powder with crushed sporoderm alcohol extract Than not spore powder with crushed sporoderm, there is the higher tumoricidal activity of external poison.

Hippocampus contains aminoacid and protein, fatty acid, steroidal and inorganic elements, sweet in flavor and warm in property, kidney invigorating and YANG supporting, eliminating mass Abdominal mass.

The health-care effect of Cornu Cervi Pantotrichum is the highest, is good general tonic.Cornu Cervi Pantotrichum contains the amino more more rich than Radix Ginseng Acid, lecithin, vitamin and trace element etc..Cornu Cervi Pantotrichum is warm in nature and the driest, has and rouses oneself and improve body function, to whole body Patient after weakness, prolonged illness, has preferable health-care effect.Cornu Cervi Pantotrichum can improve cellular immunization and the humoral immunization merit of body Can, promote the conversion of lymphocyte, there is the effect of immunopotentiating agent.It can increase body defence capability to external world, Regulate internal immunologic balance and avoid disease occur and promote wound healing, sick body rehabilitation, thus play strengthening body, The effect that opposing is old and feeble.

Testis et penis callorhini warming the kidney tonifying YANG, benefit essence is mended marrow, is cured mainly deficient impairment caused by overstrain；Sexual impotence essence declines；Premature ejaculation；Waist knee flaccidity；The heart Abdomen pain.

Chinese angelica blood supplementing is invigorated blood circulation, menstruction regulating and pain relieving, loosening bowel to relieve constipation.For blood deficiency and yellow complexion, dizziness cardiopalmus, menoxenia, warp Close dysmenorrhea, asthenia cold abdominalgia, dryness of the intestine constipation, rheumatic arthralgia, injury from falling down, ulcer sores.

Using above-mentioned each component proportion, the kidney-tonifying preparation of the present invention, while improving body immunity, also has the kidney invigorating Yang nourishing-strengthening effect.

As preferably, the preparation method of Herba Verbenae is: take Herba Verbenae medical material, pulverizes as coarse powder, adds coarse powder 8 every time The alcohol heating reflux that mass concentration is 80% of times weight extracts 2 times, each 2 hours, and united extraction liquid filters, concentrating under reduced pressure Being the concentrated solution of 1.12-1.15 to relative density at 50 DEG C, the distilled water adding 4 times of volumes of concentrated solution is uniformly dispersed, cold Hide and filter after standing, take supernatant and pass through HP-20 type macroporous adsorptive resins, first with the distilled water eluting of 4 times of column volumes, then use The mass concentration of 4 times of column volumes is the ethanol elution of 20%, discards eluent, and continuing by the mass concentration of 8 times of column volumes is 80% Ethanol elution, collects eluent, decompression recycling ethanol, concentrate drying, is ground into fine powder and must contain the horsewhip of verbenalin more than 50% Grass extract.

The inventive method is simple, and abundant raw materials easily produces, and extract verbenalin content is high, can effectively realize in system Application in standby treatment prostatitis medicine, is combined together with remaining component and can be effectively improved male urine pollakiuria not to the utmost tired Angry, start with from graft function conditioning, treating both the principal and secondary aspects of a disease.

As preferably, described Cordyceps is extracted the preparation method of powder and is: it comprises the following steps:

A. countercurrent extraction: the alcohol of the 90-98% of described to 100kg Cordyceps mycelium and 8-12 times Cordyceps mycelium weight is placed in tank Group formula counterflow extraction apparatus is carried out include that three circulations of the first circulation, the second circulation and the 3rd circulation are extracted, described tank group Share for often organizing three extraction pot series connection, countercurrent extraction flow velocity is 25-35min/ tank, and Extracting temperature is 30-80 DEG C;

Described first circulation is to start countercurrent extraction from the first extraction pot: specifically by described to Cordyceps mycelium and 8-12 times Cordyceps The alcohol of the 90-98% of mycelium quality is added in the first extraction pot, the second extraction pot and the 3rd extraction pot by equal portions respectively;First Putting into fluid reservoir after the medical filtration that extraction pot extracts for the first time standby, the second time of the first extraction pot is extracted and the first extraction pot Third time extract be separately added into 4-5 times of water;Then using the second extracting solution of the first extraction pot as the first of the second extraction pot The solvent of secondary extraction, the first extracting solution of the second extraction pot filters standby;Using the 3rd extracting solution of the first extraction pot as second The solvent that the second time of extraction pot is extracted, the second time of the second extraction pot adds 4-5 times of water in extracting;The second of second extraction pot Extracting solution is as the solvent of the first time extraction of the 3rd extraction pot;

Described second circulation is to start countercurrent extraction from the second extraction pot: specifically merge the first extraction pot the first circulation, The first extracting solution in second extraction pot and the 3rd extraction pot is to including the Cordyceps mycelium after the first circulation second time is extracted In second extraction pot of slag, it is subsequently adding 4-5 times of water and carries out extracting for the first time;First extracting solution of the second extraction pot is added bag In 3rd extraction pot of the Cordyceps mycelium slag after extracting for the first time containing the first circulation, it is subsequently adding 4-5 times of water and carries out first Secondary extraction;Then the first extracting solution of the 3rd extraction pot is added and include the Cordyceps mycelium after the first circulation third time is extracted First extraction pot of slag is carried out extract for the first time;

Described 3rd circulation is to start countercurrent extraction from the 3rd extraction pot: specifically merge the first extraction pot the second circulation, The first extracting solution in second extraction pot and the 3rd extraction pot is to including the Cordyceps mycelium after the second circulation is extracted for the first time In 3rd extraction pot of slag, it is subsequently adding 4-5 times of water and carries out extracting for the first time;First extracting solution of the 3rd extraction pot is added bag In first extraction pot of the Cordyceps mycelium slag after extracting for the first time containing the second circulation, it is subsequently adding 4-5 times of water and carries out first Secondary extraction;Then the first extracting solution of the first extraction pot is added and include the Cordyceps mycelium after the second circulation is extracted for the first time Second extraction pot of slag is carried out extract for the first time;Described alcohol is one or more in methanol, ethanol or isopropanol;

B. collect: merge the first extracting solution in the first extraction pot, the second extraction pot and the 3rd extraction pot in the 3rd circulation, Obtain just extract;

C. pre-treatment: described just extract reduced vacuum concentrated, then adds deionized water cooling standing and obtains pre-treatment in 8-15 hour Liquid;

D. filter: described pretreatment liquid filtration is filtered precipitation, takes supernatant;

E. concentrate: add in described supernatant before concentration and account for the petroleum ether of 1.5-3 times of quality of supernatant and the mixing of methanol Liquid, mixing immersion 0.5-0.8h, 40-50 DEG C, supersound extraction 2-4min under 50-70KHz;By dense for described supernatant reduced vacuum Being reduced to proportion is 1.25-1.35, obtains concentrated solution;

F. it is spray-dried: described concentrated solution is spray-dried and obtains powder-product.

The present invention uses specific countercurrent extraction, pre-treatment simultaneously, filters, concentrates and be spray-dried, and can effectively extract worm Free sterol, cordycepin, adenosine and polysaccharide in grass mycelium；More than 2/3 can be reduced under conditions of not reducing extraction ratio Extract solvent consumption, and make the combination ergosterol in extraction raw material the most free, thus improve free ergosterol Extraction efficiency.As preferably, kidney-tonifying preparation preparation method includes:

(1) merceration: described raw material powder is broken into 8-12 mesh coarse powder, adds 38-42 degree Chinese liquor 40-100 part and mixes at-30-30 DEG C Soak and obtain soak；

(2) concussion: by described soak ultrasonic wave concussion 15-30min, ultrasonic frequency is 20KHZ-80KHZ；

(3) ultraviolet irradiates: described concussion irradiates 40-80min with ultraviolet in terminating latter 1-2 hour；

(4) circulation operation: operate above-mentioned ultrasonic wave concussion and ultraviolet irradiation every circulation in 5 days, until soak time is not less than One month；

(5) purify: take supernatant and put heating in extractor, be then vacuum dried, be ground into 150-200 mesh fine powder, load capsule Or it is pressed into primordial QI reinforcing sheet acquisition kidney-tonifying preparation.

Use the method to prepare kidney-tonifying preparation, the leaching rate of material effective component can be effectively improved so that it is internal is effective Composition can give full play to coordinative role.

As preferably, during preparation, first Fructus Rubi is removed fruit stem, then freezing processing gained.

After Fructus Rubi carries out early stage process i.e. removal fruit stem, the kidney-tonifying preparation made is good in taste, remains simultaneously and covers The fragrance of basin and health care.This is likely due in Fructus Rubi fruit stem exist more lignin, and uses Fructus Rubi to go Except mixing with especially bar halberd and the Radix Astragali after fruit stem, the brewing time preparing kidney-tonifying preparation shortens, and can retain Fructus Rubi simultaneously Flavone and anthocyanidin, make kidney-tonifying preparation have good anti-oxidation health function；Inventor the most also finds the kidney invigorating prepared Preparation has promotion blood capillary blood supply function, improves helpful to body immunity.

As preferably, described cryogenic temperature is-20～-10 DEG C.

Prepare being used further to follow-up kidney-tonifying preparation after Fructus Rubi freezing, on the one hand can solve Fructus Rubi and be difficult to preserve appearance Perishable problem, reduces cost；On the other hand homogeneous kidney-tonifying preparation can be prepared.With institute freezing under this cryogenic temperature Obtaining Fructus Rubi to mix with remaining raw material, more preferably, kidney-tonifying preparation entrance is purer, and this can for the mouthfeel of the final kidney-tonifying preparation prepared again Can be because this temperature section makes microorganism effectively to be killed, and improves the quality of kidney-tonifying preparation.

As preferably, described lucid ganoderma juice adds before step (2) is shaken.

As preferably, the preparation method of described lucid ganoderma juice in turn includes the following steps:

A, first Ganoderma cleaned, cut into slices, pulverize, add in the deionized water of 5-8 times of Ganoderma weight and fully soak 12-15 hour, Then heating infusion 4-5 hour, extracts in being subsequently poured into countercurrent extraction machine, obtains coarse filtration liquid;By coarse filtration liquid with containing poly- The secondary filter film of tacryl membrane cartridge tentatively filters, and removes the fine particle in coarse filtration liquid, obtains preliminary filtrate；

B, preliminary filtrate carrying out ultrafiltration, then concentrated by ultrafiltration permeate reverse osmosis membrane, obtaining solid contents is The concentrated solution A of 5%-20%；The half taking concentrated solution A is concentrated in vacuo, and obtains the concentrated solution B that solid contents is 30%-35%; Remaining concentrated solution A and concentrated solution B is mixed to get concentrated solution C;The solids concentration of concentrated solution A is 15%-20%；Concentrated solution B Solids concentration be 33%-35%；

C, being sterilized by concentrated solution C, the temperature of sterilization is 120-140 DEG C, and the time of sterilization is the 3-5 second；

D, will sterilization after concentrated solution C carry out precipitate with ethanol filtration, specifically add the 38-42 degree pure grain rice wine of 2-3 times of concentrated solution C weight Carry out precipitate with ethanol filtration, be then heated to 50-80 DEG C and keep 0.5-0.8 hour carrying out ethanol volatilization, it is thus achieved that lucid ganoderma juice, standby.

Use the lucid ganoderma juice prepared of the present invention can the effective effective ingredient in concentration extraction Ganoderma, in order to retain color, smell and taste, The present invention selects specific solid contents, and after concentrating through reverse osmosis membrane, only takes a part of concentrated solution and carry out vacuum Concentrating, another part is not concentrated in vacuo, and is then mixed by two-part concentrated solution, and the concentrated solution so obtained both retained Color, smell and taste, can keep again certain concentration；Add lucid ganoderma juice before earthquake, health care and the battalion of kidney-tonifying preparation can be improved Form point, can guarantee that again the color, smell and taste of kidney-tonifying preparation.

As preferably, the molecular cut off of step B ultrasonic filter ultrafilter membrane used is 12-15 ten thousand dalton.

As preferably, the preparation method of described free ergosterol powder includes step successively:

1. just carrying: add the 90-98% ethanol of 8-12 times amount in 100kg Cordyceps raw material, 0.5-1.5h is extracted in 50-80 DEG C of stirring After leach, obtain the first extracting solution and the first filtering residue, the first extracting solution is standby；The 90-of 8-12 times amount is added in the first filtering residue 98% ethanol, 50-80 DEG C of stirring leaches after extracting 0.5-1.5h, obtains the second extracting solution and the second filtering residue, and the second extracting solution is standby With；Adding the 90-98% ethanol of 8-12 times amount in the second filtering residue, 50-80 DEG C of stirring is extracted after 0.5-1.5h and is leached, and obtains the Three extracting solution；Merge the first extracting solution, the second extracting solution and the 3rd extracting solution and obtain just extract；

Described Cordyceps raw material is Cordyceps mycelium or/and Chinese caterpillar fungus culture medium；

2. pre-treatment: described just extract reduced vacuum concentrated, then adds deionized water cooling standing and obtains pre-treatment in 8-15 hour Liquid；

3. filter: described pretreatment liquid filtration is filtered precipitation, takes supernatant；

4. concentrate: described supernatant reduced vacuum is concentrated into proportion is 1.25-1.35, obtains concentrated solution；

5. it is spray-dried: described concentrated solution is spray-dried and obtains free ergosterol powder-product.

The present invention uses Cordyceps mycelium or/and Chinese caterpillar fungus culture medium extracts raw material as free ergosterol, opens up Free ergosterol extracts the newly selected of raw material；Use specific just lifting manipulation, pre-treatment simultaneously, filter, concentrate and be spray-dried, The free ergosterol in extraction raw material can be made to obtain effectively extract, and make the combination ergosterol in extraction raw material go for an outing From, thus improve the extraction efficiency of free ergosterol.

Detailed description of the invention

Embodiment one

Powdered Wuling mycelia in combination 14 parts, Herba Verbenae 8 parts, Cordyceps extracts 3 parts of powder, Ganoderma 5 parts, Cordyceps sporophore 4 parts, Fructus Jujubae 10 Part, Fructus Lycii 5 parts, Herba Cynomorii 12 parts, the Radix Astragali 8 parts, Arillus Longan 2 parts, Cortex Cinnamomi 4 parts, Fructus Alpiniae Oxyphyllae 11.Preparation method includes:

(1) merceration: raw material powder is broken into 8 mesh coarse powder, adds 38 degree of Chinese liquor 40 parts and mixes immersion acquisition soak at 20 DEG C；

(2) concussion: soak is used ultrasonic wave concussion 20min, and ultrasonic frequency is 60KHZ；

(3) ultraviolet irradiates: concussion irradiates 60min with ultraviolet in terminating latter 1 hour；

(5) purify: take supernatant and put heating in extractor, be then vacuum dried, be ground into 150 mesh fine powders, load capsule or pressure Make primordial QI reinforcing sheet and obtain kidney-tonifying preparation.

Cordyceps extracts the preparation method of powder: it comprises the following steps:

A. countercurrent extraction: the alcohol of the 90% of described to 100kg Cordyceps mycelium and 8 times Cordyceps mycelium weight is placed in pot group type inverse Carrying out including that three circulations of the first circulation, the second circulation and the 3rd circulation are extracted in stream extraction equipment, described tank group is every group Three extraction pot series connection are share, and countercurrent extraction flow velocity is 25min/ tank, and Extracting temperature is 30 DEG C;

Described first circulation is to start countercurrent extraction from the first extraction pot: specifically by Cordyceps mycelium and 8 times of described Cordyceps The alcohol of the 90% of filament quality is added in the first extraction pot, the second extraction pot and the 3rd extraction pot by equal portions respectively;First extraction pot Putting into fluid reservoir after the medical filtration extracted for the first time standby, the second time of the first extraction pot extracts the 3rd with the first extraction pot Secondary extraction is separately added into 4 times of water;Then using the second extracting solution of the first extraction pot as the second extraction pot first time extract Solvent, the first extracting solution of the second extraction pot filters standby;Using the 3rd extracting solution of the first extraction pot as the second extraction pot The solvent that second time is extracted, the second time of the second extraction pot adds 4-5 times of water in extracting;Second extracting solution of the second extraction pot is made It it is the solvent extracted the first time of the 3rd extraction pot;

Described second circulation is to start countercurrent extraction from the second extraction pot: specifically merge the first extraction pot the first circulation, The first extracting solution in second extraction pot and the 3rd extraction pot is to including the Cordyceps mycelium after the first circulation second time is extracted In second extraction pot of slag, it is subsequently adding 4 times of water and carries out extracting for the first time;First extracting solution of the second extraction pot is added and comprises In 3rd extraction pot of the Cordyceps mycelium slag after having the first circulation to extract for the first time, it is subsequently adding 4 times of water and carries out carrying for the first time Take;Then the first extracting solution of the 3rd extraction pot is added and include the Cordyceps mycelium slag after the first circulation third time is extracted First extraction pot is carried out extract for the first time;

3rd circulation is to start countercurrent extraction from the 3rd extraction pot: specifically merge the first extraction pot the second circulation, second The first extracting solution in extraction pot and the 3rd extraction pot is to including the Cordyceps mycelium slag after the second circulation is extracted for the first time In 3rd extraction pot, it is subsequently adding 4 times of water and carries out extracting for the first time;First extracting solution of the 3rd extraction pot is added and includes the In first extraction pot of the Cordyceps mycelium slag after two circulations extraction for the first time, it is subsequently adding 4-5 times of water and carries out extracting for the first time; Then the first extracting solution of the first extraction pot is added and include the of the Cordyceps mycelium slag after the second circulation is extracted for the first time Two extraction pot are carried out extract for the first time;Described alcohol is one or more in methanol, ethanol or isopropanol;

C. pre-treatment: described just extract reduced vacuum concentrated, then adds deionized water cooling standing and obtains pretreatment liquid in 8 hours;

E. concentrate: in described supernatant, add petroleum ether and the mixed liquor of methanol accounting for 1.5 times of quality of supernatant before concentration, Mixing immersion 0.5h, 40 DEG C, supersound extraction 2min under 50KHz;Described supernatant reduced vacuum is concentrated into proportion is 1.25, Obtain concentrated solution;

Embodiment two

Powdered Wuling mycelia in combination 20 parts, Herba Verbenae 15 parts, Cordyceps extracts 6 parts of powder, Ganoderma 9 parts, Cordyceps sporophore 6 parts, Fructus Jujubae 15 parts, Fructus Lycii 10 parts, Herba Cynomorii 15 parts, the Radix Astragali 12 parts, Arillus Longan 6 parts, Cortex Cinnamomi 13 parts, Fructus Alpiniae Oxyphyllae 7.Preparation method includes:

(1) merceration: raw material powder is broken into 12 mesh coarse powder, adds 42 degree of Chinese liquor 100 parts and mixes immersion acquisition soak at-30 DEG C；

(2) concussion: soak is used ultrasonic wave concussion 15min, and ultrasonic frequency is 20KHZ；

(3) ultraviolet irradiates: concussion irradiates 40min with ultraviolet in terminating latter 1 hour；

(5) purify: take supernatant and put heating in extractor, be then vacuum dried, be ground into 200 mesh fine powders, load capsule or pressure Make primordial QI reinforcing sheet and obtain kidney-tonifying preparation.

A. countercurrent extraction: the alcohol of the 98% of described to 100kg Cordyceps mycelium and 12 times Cordyceps mycelium weight is placed in pot group type Carrying out in counterflow extraction apparatus including that three circulations of the first circulation, the second circulation and the 3rd circulation are extracted, described tank group is every Organizing three extraction pot series connection to share, countercurrent extraction flow velocity is 35min/ tank, and Extracting temperature is 80 DEG C;

Described first circulation is to start countercurrent extraction from the first extraction pot: specifically by Cordyceps mycelium and 12 times of described Cordyceps The alcohol of the 98% of filament quality is added in the first extraction pot, the second extraction pot and the 3rd extraction pot by equal portions respectively;First extraction pot Putting into fluid reservoir after the medical filtration extracted for the first time standby, the second time of the first extraction pot extracts the 3rd with the first extraction pot Secondary extraction is separately added into 5 times of water;Then using the second extracting solution of the first extraction pot as the second extraction pot first time extract Solvent, the first extracting solution of the second extraction pot filters standby;Using the 3rd extracting solution of the first extraction pot as the second extraction pot The solvent that second time is extracted, the second time of the second extraction pot adds 5 times of water in extracting;Second extracting solution conduct of the second extraction pot The solvent that the first time of the 3rd extraction pot extracts;

Described second circulation is to start countercurrent extraction from the second extraction pot: specifically merge the first extraction pot the first circulation, The first extracting solution in second extraction pot and the 3rd extraction pot is to including the Cordyceps mycelium after the first circulation second time is extracted In second extraction pot of slag, it is subsequently adding 5 times of water and carries out extracting for the first time;First extracting solution of the second extraction pot is added and comprises In 3rd extraction pot of the Cordyceps mycelium slag after having the first circulation to extract for the first time, it is subsequently adding 5 times of water and carries out carrying for the first time Take;Then the first extracting solution of the 3rd extraction pot is added and include the Cordyceps mycelium slag after the first circulation third time is extracted First extraction pot is carried out extract for the first time;

Described 3rd circulation is to start countercurrent extraction from the 3rd extraction pot: specifically merge the first extraction pot the second circulation, The first extracting solution in second extraction pot and the 3rd extraction pot is to including the Cordyceps mycelium after the second circulation is extracted for the first time In 3rd extraction pot of slag, it is subsequently adding 5 times of water and carries out extracting for the first time;First extracting solution of the 3rd extraction pot is added and comprises In first extraction pot of the Cordyceps mycelium slag after having the second circulation to extract for the first time, it is subsequently adding 5 times of water and carries out carrying for the first time Take;Then the first extracting solution of the first extraction pot is added and include the Cordyceps mycelium slag after the second circulation is extracted for the first time Second extraction pot is carried out extract for the first time;Described alcohol is one or more in methanol, ethanol or isopropanol;

C. pre-treatment: described just extract reduced vacuum concentrated, then adds deionized water cooling standing and obtains pretreatment liquid in 15 hours;

E. concentrate: in described supernatant, add petroleum ether and the mixed liquor of methanol accounting for 3 times of quality of supernatant before concentration, mixed Close and soak 0.8h, 50 DEG C, supersound extraction 4min under 70KHz;Described supernatant reduced vacuum is concentrated into proportion is 1.35, Obtain concentrated solution;

Embodiment three

Powdered Wuling mycelia in combination 18 parts, Herba Verbenae 12 parts, Cordyceps extracts 5 parts of powder, Ganoderma 6 parts, Cordyceps sporophore 5 parts, Fructus Jujubae 12 parts, Fructus Lycii 8 parts, Herba Cynomorii 14 parts, the Radix Astragali 10 parts, Arillus Longan 5 parts, Cortex Cinnamomi 8 parts, Fructus Alpiniae Oxyphyllae 9.Preparation method includes:

(1) merceration: raw material powder is broken into 10 mesh coarse powder, adds 40 degree of Chinese liquor 60 parts and mixes immersion acquisition soak at 30 DEG C；

(2) concussion: soak is used ultrasonic wave concussion 30min, and ultrasonic frequency is 80KHZ；

(3) ultraviolet irradiates: concussion irradiates 80min with ultraviolet in terminating latter 2 hours；

(5) purify: take supernatant and put heating in extractor, be then vacuum dried, be ground into 180 mesh fine powders, load capsule or pressure Make primordial QI reinforcing sheet and obtain kidney-tonifying preparation.

A. countercurrent extraction: the alcohol of the 92% of described to 100kg Cordyceps mycelium and 10 times Cordyceps mycelium weight is placed in pot group type Carrying out in counterflow extraction apparatus including that three circulations of the first circulation, the second circulation and the 3rd circulation are extracted, described tank group is every Organizing three extraction pot series connection to share, countercurrent extraction flow velocity is 30min/ tank, and Extracting temperature is 50 DEG C;

Described first circulation is to start countercurrent extraction from the first extraction pot: specifically by Cordyceps mycelium and 10 times of described Cordyceps The alcohol of the 95% of filament quality is added in the first extraction pot, the second extraction pot and the 3rd extraction pot by equal portions respectively;First extraction pot Putting into fluid reservoir after the medical filtration extracted for the first time standby, the second time of the first extraction pot extracts the 3rd with the first extraction pot Secondary extraction is separately added into 4.5 times of water;Then the second extracting solution of the first extraction pot was extracted as the first time of the second extraction pot Solvent, the second extraction pot first extracting solution filter standby;Using the 3rd extraction of the first extraction pot as the second extraction pot The solvent that second time is extracted, the second time of the second extraction pot adds 4.5 times of water in extracting;Second extracting solution of the second extraction pot is made It it is the solvent extracted the first time of the 3rd extraction pot;

Described second circulation is to start countercurrent extraction from the second extraction pot: specifically merge the first extraction pot the first circulation, The first extracting solution in second extraction pot and the 3rd extraction pot is to including the Cordyceps mycelium after the first circulation second time is extracted In second extraction pot of slag, it is subsequently adding 4.5 times of water and carries out extracting for the first time;First extracting solution of the second extraction pot is added bag In 3rd extraction pot of the Cordyceps mycelium slag after extracting for the first time containing the first circulation, it is subsequently adding 4.5 times of water and carries out first Secondary extraction;Then the first extracting solution of the 3rd extraction pot is added and include the Cordyceps mycelium after the first circulation third time is extracted First extraction pot of slag is carried out extract for the first time;

E. concentrate: add in described supernatant before concentration and account for the petroleum ether of 1.5-3 times of quality of supernatant and the mixing of methanol Liquid, mixing immersion 0.6h, 40-50 DEG C, supersound extraction 3min under 60KHz;Described supernatant reduced vacuum is concentrated into proportion is 1.3, obtain concentrated solution;

Embodiment four

With embodiment one, raw material except for the difference that the most also includes free 0.6 part of ergosterol powder, lucid ganoderma juice 0.3 part, Sporoderm-broken Ganoderma Lucidum Spore powder extract 0.3 part, 0.1 part of Cornu Cervi Pantotrichum, Hippocampus 0.5 part, Testis et penis callorhini 0.05 part, Fructus Rubi 1 part, bar halberd 0.3 Part, Radix Angelicae Sinensis 0.4 part.

First Fructus Rubi is removed fruit stem during preparation, then freezing processing gained；Cryogenic temperature is-20 DEG C；Lucid ganoderma juice is in step Suddenly add before (2) concussion.Take and cultivate qualified Cordyceps sporophore as Cordyceps sporophore, using Cordyceps sporophore culture bottle as city Sell bottle.

The preparation method of lucid ganoderma juice in turn includes the following steps:

A, first Ganoderma cleaned, cut into slices, pulverize, add in the deionized water of 5 times of Ganoderma weight and fully soak 12 hours, then add Hot infusion 4 hours, extracts in being subsequently poured into countercurrent extraction machine, obtains coarse filtration liquid;By coarse filtration liquid with containing polypropylene fibre The secondary filter film of membrane cartridge tentatively filters, and removes the fine particle in coarse filtration liquid, obtains preliminary filtrate；

B, preliminary filtrate being carried out ultrafiltration, the molecular cut off of ultrafilter membrane used by ultrafiltration is 120,000 dalton；Again that ultrafiltration is saturating Cross liquid reverse osmosis membrane to concentrate, obtain the concentrated solution A that solid contents is 5%；Taking the half of concentrated solution A, to carry out vacuum dense Contracting, obtains the concentrated solution B that solid contents is 30%;Remaining concentrated solution A and concentrated solution B is mixed to get concentrated solution C;Concentrate The solids concentration of liquid A is 15%；The solids concentration of concentrated solution B is 33%；

C, being sterilized by concentrated solution C, the temperature of sterilization is 120 DEG C, and the time of sterilization is 3 seconds；

D, will sterilization after concentrated solution C carry out precipitate with ethanol filtration, 38 pure grain rice wines specifically adding 2-3 times of concentrated solution C weight are carried out Precipitate with ethanol filters, and is then heated to 50 DEG C and keeps 0.5 hour carrying out ethanol volatilization, it is thus achieved that lucid ganoderma juice, standby.

The preparation of free ergosterol powder:

Chinese caterpillar fungus culture medium by weight by 20 parts of rice meals, 0.5 part of Rhizoma Chuanxiong powder, 1 part of isomaltulose, 2 parts of egg white oligopeptide havings, 2 The water of part Maifanitum, 1 part of Ganoderma powder, 0.5 part of squalane, 3 parts of pupa slurries, 0.5 portion of Radix Dauci Sativae juice, 5 parts of decoction liquor and 45 parts forms； Egg white oligopeptide having uses F value and reaches the egg white oligopeptide having of 20；

Wherein, water be pH value be the water of 7.5, and containing the zinc of 0.03mg/L, the ferrum of 0.05mg/L, the trip of 10mg/L in water From carbon dioxide, the calcium of 60mg/L and the iodide of 0.2mg/L；

Decoction liquor is to take oatmeal 3 parts, Semen Sesami Nigrum 3 parts, Semen sojae atricolor 4 parts by weight, adds 3 times of soak by water 4h, filters, by filtered solution Concentrate with reverse osmosis membrane, obtain the first decocting liquid of solid contents 8%；Take the Pseudobulbus Bletillae (Rhizoma Bletillae) 4 parts by weight, add 1 times of soak by water 3h, filters, concentrates with reverse osmosis membrane, obtain the second decocting liquid of solid contents 45%；It is then combined with the first decocting liquid and second Decocting liquid obtains decoction liquor.

Adding 90% ethanol of 8 times amount in 100kg Chinese caterpillar fungus culture medium, 50 DEG C of stirrings leach after extracting 0.5h, obtain first and carry Taking liquid and the first filtering residue, the first extracting solution is standby；Adding the 90-98% ethanol of 8 times amount in the first filtering residue, 50 DEG C of stirrings are extracted Leaching after 0.5h, obtain the second extracting solution and the second filtering residue, the second extracting solution is standby；The 90% of 8 times amount is added in the second filtering residue Ethanol, 50-80 DEG C of stirring leaches after extracting 0.5h, obtains the 3rd extracting solution；Merge the first extracting solution, the second extracting solution and the 3rd Extracting solution obtains just extract；

First extract is concentrated into 180kg in vacuum for reduced vacuum under-0.01MPa, then adds deionized water 600kg, cooling Stand 8 hours and obtain pretreatment liquid.

Pretreatment liquid is filtered precipitation, takes supernatant；

Adding petroleum ether and the mixed liquor of methanol accounting for 1.5 times of quality of supernatant in supernatant liquid, 0.5h is soaked in mixing, 40 DEG C, supersound extraction 2min under 50KHz；Then supernatant reduced vacuum being concentrated into proportion is 1.25, obtains concentrated solution；

It is spray-dried: concentrated solution is spray-dried and obtains free ergosterol powder-product.

Measure the Quantitative Determination of Ergosterol in Chinese caterpillar fungus culture medium with RP-HPLC and the final products ergosterol powder that dissociates contains Amount and purity, thus calculate the extraction ratio of free ergosterol in the method.

Computing formula is: the free ergosterol quality in the extraction ratio of free ergosterol=free ergosterol powder / (synthesizing ergosterol quality in free ergosterol quality+Chinese caterpillar fungus culture medium in Chinese caterpillar fungus culture medium)

After testing, the extraction ratio of free ergosterol is 96.7%.

Embodiment five

With embodiment two, raw material except for the difference that the most also includes free ergosterol powder 0.6-0.9 part, lucid ganoderma juice 0.9 part, Sporoderm-broken Ganoderma Lucidum Spore powder extract 0.7 part, 0.2 part of Cornu Cervi Pantotrichum, Hippocampus 0.8 part, Testis et penis callorhini 0.08 part, Fructus Rubi 2 parts, bar Halberd 0.5 part, Radix Angelicae Sinensis 0.2 part.

First Fructus Rubi is removed fruit stem during preparation, then freezing processing gained；Cryogenic temperature is-10 DEG C；Lucid ganoderma juice is in step Suddenly add before (2) concussion.Take and cultivate qualified Cordyceps sporophore as Cordyceps sporophore, using Cordyceps sporophore culture bottle as city Sell bottle.

A, first Ganoderma cleaned, cut into slices, pulverize, add in the deionized water of 8 times of Ganoderma weight and fully soak 15 hours, then add Hot infusion 5 hours, extracts in being subsequently poured into countercurrent extraction machine, obtains coarse filtration liquid;By coarse filtration liquid with containing polypropylene fibre The secondary filter film of membrane cartridge tentatively filters, and removes the fine particle in coarse filtration liquid, obtains preliminary filtrate；

B, preliminary filtrate being carried out ultrafiltration, the molecular cut off of ultrafilter membrane used by ultrafiltration is 150,000 dalton；Again that ultrafiltration is saturating Cross liquid reverse osmosis membrane to concentrate, obtain the concentrated solution A that solid contents is 20%；Taking the half of concentrated solution A, to carry out vacuum dense Contracting, obtains the concentrated solution B that solid contents is 35%;Remaining concentrated solution A and concentrated solution B is mixed to get concentrated solution C;Concentrate The solids concentration of liquid A is 20%；The solids concentration of concentrated solution B is 35%；

C, being sterilized by concentrated solution C, the temperature of sterilization is 140 DEG C, and the time of sterilization is 5 seconds；

D, will sterilization after concentrated solution C carry out precipitate with ethanol filtration, the 42 degree of pure grain rice wines specifically adding 3 times of concentrated solution C weight are carried out Precipitate with ethanol filters, and is then heated to 80 DEG C and keeps 0.8 hour carrying out ethanol volatilization, it is thus achieved that lucid ganoderma juice, standby.

The preparation of free ergosterol powder:

Chinese caterpillar fungus culture medium by weight by 28 parts of rice meals, 1.5 parts of Rhizoma Chuanxiong powder, 3 parts of isomaltuloses, 5 parts of egg white oligopeptide havings, 5 Part Maifanitum, 1.8 parts of Ganoderma powder, 1.1 parts of squalanes, 8 parts of pupa slurries, 1.5 portions of Radix Dauci Sativae juices, 10 parts of decoction liquor and the water of 55 parts Composition；Egg white oligopeptide having uses F value and reaches the egg white oligopeptide having of 30；

Water be pH value be the water of 7.7, and in water containing the zinc of 0.1mg/L, the ferrum of 0.08mg/L, free the two of 12mg/L Carbonoxide, the calcium of 70mg/L and the iodide of 0.2mg/L；

Decoction liquor is to take oatmeal 7 parts, Semen Sesami Nigrum 5 parts, Semen sojae atricolor 8 parts by weight, adds 5 times of soak by water 5h, filters, by filtered solution Concentrate with reverse osmosis membrane, obtain the first decocting liquid of solid contents 15%；Take the Pseudobulbus Bletillae (Rhizoma Bletillae) 6 parts by weight, add 2 times of soak by water 5h, filters, concentrates with reverse osmosis membrane, obtain the second decocting liquid of solid contents 55%；It is then combined with the first decocting liquid and second Decocting liquid obtains decoction liquor.

Adding 98% ethanol of 12 times amount in 100kg Chinese caterpillar fungus culture medium, 80 DEG C of stirrings leach after extracting 1.5h, obtain First extracting solution and the first filtering residue, the first extracting solution is standby；98% ethanol of 12 times amount, 80 DEG C of stirrings are added in the first filtering residue Leaching after extracting 1.5h, obtain the second extracting solution and the second filtering residue, the second extracting solution is standby；12 times amount are added in the second filtering residue 98% ethanol, 80 DEG C of stirrings are extracted after 1.5h and are leached, obtain the 3rd extracting solution；Merge the first extracting solution, the second extracting solution and 3rd extracting solution obtains just extract；

First extract is concentrated into 220kg in vacuum for reduced vacuum under-0.03MPa, then adds deionized water 1000kg, cooling Stand 15 hours and obtain pretreatment liquid.

Pretreatment liquid is filtered precipitation, takes supernatant；

Adding petroleum ether and the mixed liquor of methanol accounting for 3 times of quality of supernatant in supernatant liquid, 0.8h is soaked in mixing, 50 DEG C, supersound extraction 4min under 70KHz；Then supernatant reduced vacuum being concentrated into proportion is 1.35, obtains concentrated solution；

Detection and computational methods are with embodiment one, and after testing, the extraction ratio of free ergosterol is 96.9%.

Embodiment six

With embodiment three, raw material except for the difference that the most also includes free ergosterol powder 0.6-0.9 part, lucid ganoderma juice 0.6 part, Sporoderm-broken Ganoderma Lucidum Spore powder extract 0.5 part, 0.12 part of Cornu Cervi Pantotrichum, Hippocampus 0.6 part, Testis et penis callorhini 0.07 part, Fructus Rubi 1.2 Part, bar halberd 0.4 part, Radix Angelicae Sinensis 0.3 part.

First Fructus Rubi is removed fruit stem during preparation, then freezing processing gained；Cryogenic temperature is-15 DEG C；Lucid ganoderma juice is in step Suddenly add before (2) concussion.Take and cultivate qualified Cordyceps sporophore as Cordyceps sporophore, using Cordyceps sporophore culture bottle as city Sell bottle.

A, first Ganoderma cleaned, cut into slices, pulverize, add in the deionized water of 6 times of Ganoderma weight and fully soak 13 hours, then add Hot infusion 4.5 hours, extracts in being subsequently poured into countercurrent extraction machine, obtains coarse filtration liquid;By coarse filtration liquid with fine containing polypropylene The secondary filter film of dimension membrane cartridge tentatively filters, and removes the fine particle in coarse filtration liquid, obtains preliminary filtrate；

B, preliminary filtrate being carried out ultrafiltration, the molecular cut off of ultrafilter membrane used by ultrafiltration is 130,000 dalton；Again that ultrafiltration is saturating Cross liquid reverse osmosis membrane to concentrate, obtain the concentrated solution A that solid contents is 15%；Taking the half of concentrated solution A, to carry out vacuum dense Contracting, obtains the concentrated solution B that solid contents is 33%;Remaining concentrated solution A and concentrated solution B is mixed to get concentrated solution C;Concentrate The solids concentration of liquid A is 18%；The solids concentration of concentrated solution B is 34%；

C, being sterilized by concentrated solution C, the temperature of sterilization is 130 DEG C, and the time of sterilization is 4 seconds；

D, will sterilization after concentrated solution C carry out precipitate with ethanol filtration, the 39 degree of pure grain rice wines specifically adding 2.3 times of concentrated solution C weight enter Row precipitate with ethanol filters, and is then heated to 60 DEG C and keeps 0.6 hour carrying out ethanol volatilization, it is thus achieved that lucid ganoderma juice, standby.

The preparation of free ergosterol powder:

Chinese caterpillar fungus culture medium by weight by 25 parts of rice meals, 1 part of Rhizoma Chuanxiong powder, 2 parts of isomaltuloses, 3 parts of egg white oligopeptide havings, 4 parts Maifanitum, 1.5 parts of Ganoderma powder, 0.8 part of squalane, 5 parts of pupa slurries, 0.9 portion of Radix Dauci Sativae juice, 8 parts of decoction liquor and the water composition of 49 parts； Egg white oligopeptide having uses F value and reaches the egg white oligopeptide having of 25；

Water be pH value be the water of 7.6, and in water containing the zinc of 0.06mg/L, the ferrum of 0.07mg/L, free the two of 11mg/L Carbonoxide, the calcium of 65mg/L and the iodide of 0.15mg/L；

Decoction liquor is to take oatmeal 4 parts, Semen Sesami Nigrum 4 parts, Semen sojae atricolor 5 parts by weight, adds 4 times of soak by water 4.5h, filters, and will filter Liquid reverse osmosis membrane concentrates, and obtains the first decocting liquid of solid contents 10%；Take the Pseudobulbus Bletillae (Rhizoma Bletillae) 5 parts by weight, add 1.5 times of water Decoct 4h, filter, concentrate with reverse osmosis membrane, obtain the second decocting liquid of solid contents 49%；Be then combined with the first decocting liquid and Second decocting liquid obtains decoction liquor.

Adding 95% ethanol of 10 times amount in 100kg Chinese caterpillar fungus culture medium, 70 DEG C of stirrings are filtered with 30-50 mesh after extracting 1h Net leaches, and obtains the first extracting solution and the first filtering residue, and the first extracting solution is standby；95% second of 10 times amount is added in the first filtering residue Alcohol, 70 DEG C of stirrings leach with 30-50 mesh filter screen after extracting 1h, obtain the second extracting solution and the second filtering residue, and the second extracting solution is standby； Adding 95% ethanol of 10 times amount in the second filtering residue, 70 DEG C of stirrings leach with 30-50 mesh filter screen after extracting 1h, obtain the 3rd and carry Take liquid；Merge the first extracting solution, the second extracting solution and the 3rd extracting solution and obtain just extract.

First extract is concentrated into 200kg in vacuum for reduced vacuum under-0.02MPa, then adds deionized water 800kg, Cooling stands 10 hours and obtains pretreatment liquid.

Pretreatment liquid is filtered precipitation, takes supernatant；

Adding petroleum ether and the mixed liquor of methanol accounting for 1.5 times of quality of supernatant in supernatant liquid, 0.5h is soaked in mixing, 40 DEG C, supersound extraction 2min under 50KHz；Then supernatant is concentrated into for reduced vacuum under-0.08MPa by supernatant in vacuum Proportion is 1.3, obtains concentrated solution；

Concentrated solution is spray-dried and obtains free ergosterol powder-product.

Detection and computational methods are with embodiment one, and after testing, the extraction ratio of free ergosterol is 97.3%.

Comparative example one

With embodiment one, except for the difference that Fructus Lycii 5 parts, do not contain Herba Cynomorii, use 52 degree of Chinese liquor, do not carry out during preparation shaking and ultraviolet Line irradiates.

Comparative example two

With embodiment four, Fructus Rubi is not removed when except for the difference that preparing fruit stem, and does not wherein contain Radix Angelicae Sinensis；Time prepared by lucid ganoderma juice Do not carry out precipitate with ethanol filtration.

Comparative example three

With embodiment six, not being both and wherein do not contain powdered Wuling mycelia in combination, Herba Verbenae, Cordyceps extracts powder and Fructus Alpiniae Oxyphyllae.

The male more weak by the 100 of voluntary participation body constitution carries out double blind experiment, oral, day dose 50-100ml, every day 1-2 Secondary, a course for the treatment of is 30 days.

Data are checked by third party and add up, and shielding interference allows data more objective.Composition evaluation group marks And average.Evaluate scores and be shown in Table 1.

The product of embodiment and comparative example marked and analyzes, marking minimum 1 point, representing and do not feel Any change；Scoring is up to 10 points, represents that body constitution strengthens, and immunity improves, and people feels the most spiritual, and has tonifying YANG effect. Tonifying YANG effect makes moderate progress with the phenomenon having that waking up at obvious Morning erection or night erects and be effective record, frequent micturition and urine retention.

This specific embodiment is only explanation of the invention, and it is not limitation of the present invention, and those skilled in the art exist As required the present embodiment can be made after reading this specification and there is no the amendment of creative contribution, but as long as in the present invention Right in all protected by Patent Law.

Claims

1. a kidney-tonifying preparation, it is characterised in that include the raw material of following weight portion: powdered Wuling mycelia in combination 14-20 part, Herba Verbenae is extracted Thing 8-15 part, Cordyceps extraction powder 3-6 part, Ganoderma 5-9 part, Cordyceps sporophore 4-6 part, Fructus Jujubae 10-15 part, Fructus Lycii 5-10 part, Herba Cynomorii 12-15 part, Radix Astragali 8-12 part, Arillus Longan 2-6 part, Cortex Cinnamomi 4-13 part, Fructus Alpiniae Oxyphyllae 7-11 part.

A kind of kidney-tonifying preparation the most according to claim 1, it is characterised in that: the preparation method of described Herba Verbenae For: taking Herba Verbenae medical material, pulverize as coarse powder, the alcohol heating reflux that mass concentration is 80% every time adding 8 times of weight of coarse powder extracts 2 times, each 2 hours, united extraction liquid, filter, being evaporated to relative density at 50 DEG C is the concentrated solution of 1.12-1.15, The distilled water adding 4 times of volumes of concentrated solution is uniformly dispersed, and cold preservation is filtered after standing, and takes supernatant and is inhaled by HP-20 type macropore Attached resin column, first with the distilled water eluting of 4 times of column volumes, then with the ethanol elution that mass concentration is 20% of 4 times of column volumes, abandons Remove eluent, continue with the ethanol elution that mass concentration is 80% of 8 times of column volumes, collect eluent, decompression recycling ethanol, concentrate Dry, pulverize into fine powder and must contain the Herba Verbenae of verbenalin more than 50%.

A kind of kidney-tonifying preparation the most according to claim 2, it is characterised in that: described raw material the most also includes dissociate Ergosterol powder 0.6-0.9 part, lucid ganoderma juice 0.3-0.9 part, Sporoderm-broken Ganoderma Lucidum Spore powder extract 0.3-0.7 part, Cornu Cervi Pantotrichum 0.1- 0.2 part, Hippocampus 0.5-0.8 part, Testis et penis callorhini 0.05-0.08 part, Fructus Rubi 1-2 part, bar halberd 0.3-0.5 part, Radix Angelicae Sinensis 0.2-0.4 Part.

A kind of kidney-tonifying preparation the most according to claim 3, it is characterised in that: the preparation method of kidney-tonifying preparation includes:

(4) circulation operation: operate above-mentioned ultrasonic wave concussion and ultraviolet irradiation every circulation in 3-5 days, until soak time is the least In one month；

A kind of kidney-tonifying preparation the most according to claim 4, it is characterised in that: first Fructus Rubi is removed fruit stem during preparation, so Rear freezing processing gained.

A kind of kidney-tonifying preparation the most according to claim 5, it is characterised in that: described cryogenic temperature is-20～-10 DEG C.

A kind of kidney-tonifying preparation the most according to claim 4, it is characterised in that: described Cordyceps extracts the preparation method of powder For: it comprises the following steps:

A kind of kidney-tonifying preparation the most according to claim 7, it is characterised in that: the preparation method of described lucid ganoderma juice includes successively Following steps:

A kind of kidney-tonifying preparation the most according to claim 8, it is characterised in that: retaining point of step B ultrasonic filter ultrafilter membrane used Son amount is 12-15 ten thousand dalton.

A kind of kidney-tonifying preparation the most according to claim 3, it is characterised in that: the preparation of described free ergosterol powder Method includes step successively: