CN105998752A - Traditional Chinese medicine composition for preventing and treating vascular dementia - Google Patents
Traditional Chinese medicine composition for preventing and treating vascular dementia Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for preventing and treating vascular dementia. The traditional Chinese medicine composition for preventing and treating vascular dementia comprises ginseng, gastrodia elata, Euonymus alatus and Ligusticum wallichii. The composition can be prepared into Chinese herbal preparation crude drug for preventing and treating vascular dementia. The ginseng and the Ligusticum wallichii are extracted with alcohol, the gastrodia elata and the Euonymus alatus are extracted with water, the obtained alcohol extract and the obtained water extract are mixed, the mixture is dried under vacuum and then is pulverized to obtain the final product, and the composition can be directly taken orally or is prepared into other dosage forms such as granules, capsules, soft capsules, electuaries, pills and decocted extracts. The composition is completely prepared from natural medicinal plants, does not contain hormones, and does not contain other chemical synthetic products such as pigment, and even if a patient orally takes the composition continuously, no toxic and side effect is performed on the human body. The traditional Chinese medicine composition contains various easily absorbable ingredients such as ginsenoside, chuanxiongol, chuanxiongzine, gastrodin and quercetin, can be effectively used for treating the vascular dementia, increases cerebral blood flow, reduces cerebral vascular resistance, improves body immunocompetence, and has effects of tonifying deficiency, promoting intelligence, suppressing the hyperactive liver, subsiding Yang, dispelling blood stasis and dredging collaterals.
Description
Technical field
The present invention relates to, the Chinese medicine composition that a kind for the treatment of of vascular is dull-witted.
Background technology
Vascular dementia (vascular dementia, VD) is one group of intelligence caused by cerebrovascular disease and recognizes
Know dysfunction syndrome, be one of the common disease of senile dementia.VD morbidity is more clear and definite, tool
Having potential preventing and treating property, be otherwise known as " cataphrenia ", early diagnosis and early treatment are for intelligence
Improve or reverse especially pronounced.Along with the aging process of population, the sickness rate of VD becomes in substantially rising
Gesture, serious threat the healthy of middle-aged and elderly people and life quality, is brought to patient, family and society
Long-term misery and burden, cause the extensive concern of society and medical profession.By 2010, world's model
The prevalence of dementia enclosing interior over-65s crowd is 15%, and wherein 9% is AD, and 4% is VD.Apoplexy
VD annual morbidity about 8.5% in crowd.And the prevalence survey of China VD shows, more than 60 years old old
People's prevalence of dementia is 3.96%, and VD accounts for 2/3, and the apoplexy patient that there are about 30% is likely to occur hypophrenia.
Find effective medicine, in early days effectively preventing and treating can delay disease process, improve simultaneous phenomenon,
Improve the quality of life of patient, there is important social reality meaning undoubtedly.
Different according to the cause of disease and pathology, the classification of VD mainly includes dementia with multiple cerebral infarction, multiple
Lacunar infarct is dull-witted, massive cerebral infarction is dull-witted, binswanger disease (Binswanger
Sick), cerebral amyloid angiopathy is dull-witted, ischemia and Hypoxic Low perfusion syndrome cause dementia, hemorrhagic
The dementia etc. that pathological changes causes.Diagnosis vascular dementia, it is necessary to possess dementia symptom, history of stroke, transience
Cerebral ischemia attack history and focal nervous system signs, CT or MRI checks and confirms focal focus in brain；
Dementia must take place at after stroke onset in 3 months, and dementia symptom can occur suddenly or slowly be in progress,
The course of disease is undulatory property or steps progress.
VD is the concept in modern medicine, although traditional medicine to dull-witted understanding relatively early, but to VD
The most systematic discussion, description thereof is still dispersed in the disease such as " slow-witted card ", " forgetful ", " forgetful "
In.China Association of Traditional Chinese Medicine's gerontology can formulate " diagnosis of Research of Senile Dementia Treated, differentiation of symptoms and signs for classification of syndrome in nineteen ninety
And efficacy assessment standard " clearly VD is incorporated the category of " Research of Senile Dementia Treated ".Chinese medicine is relevant dull-witted
Discussion see " Plain Questions regulating menstruation opinion " cloud the earliest: " blood and under, gas in upper, random and forgetful "；" scape
Yue Quanshu " to propose for the first time dementia be independence disease, has substantially framework, book neutrality to have its understanding beginning
" demented, dull-witted " specially piece, it is provided that " dull-witted card, all usually without expectorant, and or with pent-up, or with unsuccessful,
Or to ponder over, or with in terror and gradually to dull-witted, its card is the most very strange, penetrates everywhere ".Although dementia
Symptom numerous and complicated, but be totally characterized with " slow-witted and stupid ".Until clear YE Tian shi " face card guide
Case ": " apoplexy from the beginning of, the slow-witted enuresis of god, old man faint in obviously ".Explicitly point out apoplexy and can cause intelligence
Go down.Contemporary, Shen Jin chela also has the note of " forgetful after apoplexy " in " miscellaneous diseases source stream rhinoceros candle apoplexy "
Carrying, everybody the most gradually recognizes that some type of dementia and apoplexy also exist internal relation, but in Chinese medicine
In do not make strict differentiation with other types dementia, therefore the most still together with other forms of dementia and claim always
" dull-witted ", " dementia ".Making a general survey of various schools, VD is deficiency in origin and excess in superficiality disease, and deficiency in origin is kidney essense, gas
Anemia is empty, mark that actually expectorant is turbid, blood stasis, turbid poison individually or mutually knot stagnant in venation, deficiency in origin and the mark mutual shadow of reality
Ringing, mutually convert, form the pathological state of vicious cycle, above disturb clear key cause brains and lose foster, clear spirit is disturbed
And send out as dementia.
Along with the increase of the aged, the problem of preventing and treating of vascular dementia has become domestic and international medical circle and has been closed
The problem of note, current modern medicine there is no ideal medicament.Therefore, play TCM Features and advantage, right
This disease makes effective diagnosis, prevention and remedy measures, treats this to solving senile dementia
One global problem, has important clinical value.
The Chinese patent of publication number CN101683368A (publication date 2010.03.31) discloses in one
Drug composition, including Rhizoma Chuanxiong, Radix Angelicae Sinensis and natural Broneolum Syntheticum, has anti-stroke and sequela thereof, improves heart and brain
The functions such as blood supply.
The Chinese patent of publication number CN101856468A (publication date 2010.10.13) discloses one and controls
Treat the pharmaceutical composition of senile dementia, including Rhizoma Gastrodiae total glycosides and uncaria total alkaloids, multiple for Post stroke
Infarct dementia has certain therapeutic effect.
Summary of the invention
In order to efficiently and effectively prevent and treat vascular dementia disease, old especially for vascular
The symptoms such as dementia patient's early memory power goes down, the invention provides in a kind for the treatment of of vascular dementia
Drug composition, it is possible to be effectively improved sensing memory, learning by association, image is recalled, random shape is re-recognized
And the ability such as portrait memory.
The Chinese medicine composition that the treatment of vascular that the present invention provides is dull-witted, including Radix Ginseng, Rhizoma Gastrodiae, Ramulus Euonymi
And Rhizoma Chuanxiong.
With Radix Ginseng as monarch in side, there is strongly invigorating primordial QI, the merit of increasing intelligence of calming the nerves, be for assertive evidence blood stasis due to qi deficiency
And set；Rhizoma Gastrodiae is that minister has suppressing the hyperactive liver to relieve the wind syndrome, nourishing YIN for suppressing the hyperactive YANG, the merit of promoting blood circulation to restore menstrual flow, put down first its high upper it
Sun, prevents the change of its yang hyperactivity wind-transformation first, has the meaning of " preventive treatment of disease "；Ramulus Euonymi is that assistant has promoting the circulation of blood dredging collateral,
The merit of dissipating blood stasis, can push away Chen Zhixin, recovers crystal's balance, makes Radix Ginseng vivaciously smooth flourish and does not stop up greasy.Rhizoma Chuanxiong is
Making guiding drug of holding concurrently, have blood circulation promoting and blood stasis dispelling, the merit of circulation of qi promoting, it is the gas medicine in blood, and all medicines can be led directly to enter blood system,
Benefaction head again, can carry all medicines and directly enter brain key；All medicines share, and play qi-restoratives Fructus Alpiniae Oxyphyllae, suppressing the hyperactive liver and subsiding YANG altogether, change
The merit of blood stasis and smoothing collaterals.
Preferably, the Chinese medicine composition that above-mentioned treatment of vascular is dull-witted, count by weight, each component
Consumption is Radix Ginseng 6～12 parts, Rhizoma Gastrodiae 6～12 parts, Ramulus Euonymi 6～12 parts, Rhizoma Chuanxiong 3～9 parts.
Preferably, the Chinese medicine composition that above-mentioned treatment of vascular is dull-witted, count by weight, each component
Consumption is Radix Ginseng 9 parts, 9 parts of Rhizoma Gastrodiae, Ramulus Euonymi 9 parts, Rhizoma Chuanxiong 6 parts.
Present invention also offers the Chinese medicine preparation crude drug that a kind for the treatment of of vascular is dull-witted, it uses to take up an official post
Chinese medicine composition described in one, is prepared by the following method:
Radix Ginseng and Rhizoma Chuanxiong mixing, add 70% (v/v) ethanol water reflux, extract, of 8 times of weight, collect leaching
Go out liquid, in Radix Ginseng and Rhizoma Chuanxiong, add 70% (v/v) ethanol water reflux, extract, of 4 times of weight the most again,
Collecting leachate, the mixing of two-stage leaching liquid concentrates, and obtains alcohol extraction thick paste；
Rhizoma Gastrodiae and Ramulus Euonymi mixing, add the soak by water of 10 times of weight, filter, add the water of 8 times of weight
Continue to decoct, filter, merge the filtrate of twice, obtain water after concentrating under reduced pressure and carry thick paste；
Alcohol extraction thick paste and water carry thick paste merge, vacuum drying, pulverize.
The effective ingredient ligustilide of Rhizoma Chuanxiong, ligustrazine, phenolic acid (salvianolic acid B, ferulic acid)
Vascular dementia is had preferable therapeutical effect, also there is obvious coronary artery dilator, increase coronary artery
The pharmacological actions such as blood flow, lax vascular smooth muscle, reduction surface activity of blood platelet.Simultaneously ligustrazine,
The extracting method of ferulic acid uses alcohol extraction content higher.The Radix Ginseng active component for the treatment of of vascular dementia is
Ginsenoside, ginsenoside has biological activity widely, can improve the quality of life of patient, extend life
Life has good effect, and the alcohol extraction thing content of ginsenoside is higher, and Radix Ginseng and Rhizoma Chuanxiong use above-mentioned
The method of alcohol extraction can effective component extracting to greatest extent.
Rhizoma Gastrodiae has effect of relieve dizziness, high fever, infantile convulsions, epilepsy, etc. arresting convulsion, calming soporific, be mainly used in treatment have a dizzy spell, wind syndrome of head head
Bitterly, numb limbs and tense tendons, insomnia, infantile convulsion etc..The effective ingredient of Rhizoma Gastrodiae typically comes with gastrodine, Rhizoma Gastrodiae total glycosides
Evaluating, gastrodine molecular weight, polarity is relatively big, uses water extraction to have technique simple, extracts content
Higher advantage.The main component Quercetin of Ramulus Euonymi, dulcitol water extraction content are relatively stable, so,
The method of Rhizoma Gastrodiae and Ramulus Euonymi employing water extraction is the most useful.
It is highly preferred that the Chinese medicine preparation crude drug that above-mentioned treatment of vascular is dull-witted, Radix Ginseng and Rhizoma Chuanxiong are for the first time
Return time is 2.0 hours, and return time is 1.5 hours for the second time.The return time of ethanol extraction controls
At 2.0 and 1.5 hours, the most fully effective component extracting.
It is highly preferred that the Chinese medicine preparation crude drug that above-mentioned treatment of vascular is dull-witted, Rhizoma Gastrodiae and Ramulus Euonymi first
Secondary decocting time is 2.0 hours, and decocting time is 1.5 hours for the second time.The time of water boiling and extraction controls
At 2.0 and 1.5 hours, the most fully effective component extracting.
It is highly preferred that the Chinese medicine preparation crude drug that above-mentioned treatment of vascular is dull-witted, the temperature of described concentrating under reduced pressure
Degree condition is 60 DEG C.The too high effective ingredient that is easily destroyed of temperature, temperature too low concentration speed is slow, with 60 DEG C
Optimum.
Above-described crude drug, both can directly be taken, it is also possible to prepared by conventional formulations again
Become other dosage forms multiple, such as pill, tablet, electuary, granule, capsule, soft capsule, drop pill,
Etc..
Compared with prior art, the method have the advantages that
The Chinese medicine composition of the present invention, adds Radix Ginseng and Ramulus Euonymi flavour of a drug, has qi-restoratives Fructus Alpiniae Oxyphyllae, and blood stasis dispelling is led to
Effect of network.Radix Ginseng mainly contains ginsenoside and organic acid and esters, has learning memory disorder and changes
Being apt to and raising effect, the most also have the effect of resisting fatigue, the maincenter that significantly improves is intended choline activity and intends youngster
Tea phenol amine activity, can strengthen choline systemic-function, increases cerebral blood flow and brain energy metabolism.Add Rhizoma Chuanxiong
Containing main components such as ligustilide, chuanxingol, ligustrazine and ferulic acids, have and improve coronary flow
Effect, has obvious sedation to central nervous system.Ramulus Euonymi contains the thing such as Quercetin, dulcitol
Matter, (20%) Han satisfied fatty acid in seed oil, there is regulating lipid metabolism and alleviate atherosclerosis
(AS) effect of lesion degree.And the cognitive function barrier that vascular dementia is mainly caused by cerebrovascular disease
Hindering syndrome, therefore this medicine composition is more targeted, improves patient while preventing and treating cerebrovascular
Cognitive dysfunction.Several herbal medicines share has integrated control cerebrovascular disease, improves the cognition of patient
Function.
Crude drug uses the technique that traditional method and modernism combine, easy to operate, the most effectively becomes
Divide and retain to greatest extent.
Detailed description of the invention
In order to make those skilled in the art be more fully understood that the present invention program, below in conjunction with specific embodiment party
The present invention is described in further detail for formula.
Embodiment 1 joins the preparation of fiber crops Yizhi Decoction
Weigh Radix Ginseng 9 parts, 9 parts of Rhizoma Gastrodiae, Ramulus Euonymi 9 parts, Rhizoma Chuanxiong 6 parts by weight.
The technique preparation of medicine uses traditional method and modernism to combine, and concrete process is as follows:
Mixing prepared in proportion by Radix Ginseng, Rhizoma Chuanxiong, uses 70% (v/v) ethanol water adding 8 times of weight to return
Stream extracts 2 hours, collects leachate, adds 4 times of weights the most again in remaining Radix Ginseng Rhizoma Chuanxiong mixture
70% (v/v) ethanol water reflux, extract, of amount 1.5 hours, collects leachate, and two-stage leaching liquid merges,
It is evaporated to paste, obtains alcohol extraction thick paste.
Rhizoma Gastrodiae, Ramulus Euonymi prepare mixing in proportion, and in employing tank, the mode of boiling is extracted, and first adds 10
The soak by water of times weight 2.0 hours, filters, and residue Rhizoma Gastrodiae and Ramulus Euonymi add the water of 8 times of weight and continue
Continuous decoction 1.5 hours, filters, merges the filtrate of twice, and 60 DEG C are evaporated to paste, obtain water and carry
Thick paste.
Second time adds the amount of ethanol water, and the multiple of the amount added water is with crude drug just weight as radix
Calculate, belong to computational methods generally in the art.
Above-mentioned alcohol extraction thick paste and water carry thick paste merge, vacuum drying, obtain dry extract, pulverize.Gained produces
Product named ginseng fiber crops Yizhi Decoction.
Embodiment 2 joins the toxicological study of fiber crops Yizhi Decoction
Ginseng fiber crops Yizhi Decoction side's acute toxicity testing (maximum dosage-feeding experiment)
1. laboratory animal selects 4 week old cleaning grade ICR mice 40, male and female half and half, body weight 20g～28g,
Purchased from Beijing Vital River Experimental Animals Technology Co., Ltd., the quality certification number: SCXK (capital) 2011-0004.
Test after adaptation is fed one week in clean laboratory animal room.
2. ginseng fiber crops Yizhi Decoction fluid extract, crude drug content is 4.00g/g, by China TCM Academy of Sciences Xiyuan Hospital
TCM Preparation Room prepare (preparation method is shown in embodiment 1).Test front distilled water to be configured to containing crude drug concentration
2.00g/ml medicinal liquid.
3. 40 mices are divided into 2 according to the method for random (application SPSS software) by packet and administration
Group, i.e. matched group and administration group, often group 20, male and female half and half.Administration group with No. 16 gavage syringe needles, 24
Maximum volume (0.4ml/10g body weight) gastric infusion that once can bear with mouse stomach in h.Prohibit before gavage
Food 12h, can't help water, is administered conventional feeds after 4h, observes 14d, integral dose 1120.0g crude drug/kg
(about 170 times of quantity), matched group gavages same volume distilled water, and method is with administration group.
4. observe mice gross morphology outward appearance, behavioral activity, diet situation, feces shape etc., and every day
Carry out experimental record, Continuous Observation in 4h after administration, the 2nd～the morning of 14d, afternoon respectively observe 1 time.
Administration every day is front and 15d measures Mouse Weight the morning.Dissect dead animal in time, observe organ disease feelings
Condition, takes and is observed visually the internal organs of pathological changes and carries out pathology film-making inspection；Observation period terminates, at cervical dislocation
Dead all animals, Systematic anatomy, take and be observed visually the internal organs of pathological changes and carry out pathology film-making inspection.
It is generally believed that according to the weight, mice in 1 day maximum dosage-feeding be equivalent to clinical daily dose of adult
100 times of amount are above safer.Result shows, Continuous Observation 14d after administration, administration group 20
Mice has no significant discomfort or death upon administration.After administration, the mice mental status is normal, hair color white and
Glossy, two is the most normal.After 14d, mice takes off cervical vertebra and puts to death and dissect, abdominal cavity, thoracic cavity, cranial cavity
And main organs (heart, liver, spleen, lung, kidney) is showed no obvious macroscopic pathological change.Examination
Testing period, survival mice body weight increases normal, and during statistical analysis, observation, Mouse Weight becomes
Change male and female between two groups there are no significant difference (P ＞ 0.05).Therefore, taking under routine dose is that safety can
Lean on.
Embodiment 3. joins the drug efficacy study of fiber crops Yizhi Decoction
1 ginseng fiber crops Yizhi Decoction blood vessel blocking method causes the impact of VD
Pharmacodynamic study uses 4-blood vessel blocking method (4-VO method) VD model, from multiple links: behavior
Learn (water maze laboratory), apoptosis and controlling gene expression, inflammatory factor level, oxidative stress etc. because of
Element research this medicine primary and secondary target spot action character and contact, illustrate its multipath, Mutiple Targets integration intervention
The curative effect of VD and mechanism.
Animal is grouped: blank group, gives isopyknic normal saline；
Sham operated rats, gives isopyknic normal saline；
Model group, gives isopyknic normal saline；
Donepezil group, by 0.51g/d gavage, (gavage Western medicine donepezil hydrochloride, is equivalent to face
Bed dose,equivalent)；
Chinese medicine group, by 1.034g crude drug/d gavage (being equivalent to clinical equivalent dosage)；
Group in Chinese medicine, by 5.17g crude drug/d gavage (being equivalent to 5 times of clinical equivalent dosage)；
Big group of Chinese medicine, by 10.34g crude drug/d gavage (being equivalent to 10 times of clinical equivalent dosage).
The 1.1 ginseng fiber crops Yizhi Decoction impacts on treated rats in Morris water maze performance
Comparing with normal group, model group either wears platform number of times, fourth quadrant distance or fourth quadrant time
All there is notable significant difference (P < 0.01)；Rats in sham-operated group is all without notable significant difference (P > 0.05).
Compared with model group rats, donepezil group, Chinese medicine are organized and either wears platform number of times, fourth quadrant distance also
Being all to have notable significant difference (P < 0.05 or P < 0.01) the fourth quadrant time, platform worn by big group of Chinese medicine
Number, fourth quadrant distance have notable significant difference (P < 0.05).The results are shown in Table 1.
The comparison of cognitive ability of rat respectively organized by table 1.
| Group | n | Wear platform number of times (secondary) | Fourth quadrant distance (cm) | Fourth quadrant time (s) |
| Normal group | 8 | 9.09±1.38 | 167.04±33.97 | 78.23±14.50 |
| Sham operated rats | 8 | 8.88±0.51 | 140.81±97.04 | 76.64±10.78 |
| Model group | 7 | 6.22±0.96■■▲▲ | 73.97±22.48■■▲ | 66.22±9.26■ |
| Donepezil group | 8 | 8.57±1.66●● | 146.54±40.70● | 77.56±12.44● |
| Chinese medicine group | 7 | 7.25±1.56■■▲* | 135.56±86.33 | 75.55±6.86 |
| Group in Chinese medicine | 8 | 7.59±0.79■▲● | 157.43±65.09● | 77.38±6.88● |
| Big group of Chinese medicine | 7 | 7.64±1.54■▲● | 142.13±47.07● | 72.08±10.15 |
Note: compare with normal group,■P ＜ 0.05,■■P ＜ 0.01；Compare with sham operated rats,▲P ＜ 0.05,▲▲P ＜ 0.01；Compare with model group,●P ＜ 0.05,●●P ＜ 0.01；Compare with donepezil group,**P ＜
0.01
1.2 ginseng fiber crops Yizhi Decoctions are on NOS, IL-1, the impact of TNF-α content in rat hippocampus
Compare with normal group, model group nitricoxide synthase (NOS), interleukin-1 (IL-1), swollen
Tumor tumor necrosis factor-alpha (TNF-α) content all has notable significant difference (P < 0.01)；Sham operated rats is big
Mus is all without notable significant difference (P > 0.05).Compared with model group, the NOS content of each medication group all has
Notable significant difference (P < 0.05 or P < 0.01), the TNF-α content of each Chinese medicine intervention group has notable statistics
Learn difference (P < 0.01).The results are shown in Table 2.
NOS, IL-1, the comparison of TNF-α content in rat hippocampus respectively organized by table 2.
| Group | n | NOS(U/ml) | IL-1(ng/L) | TNF-α(ng/L) |
| Normal group | 8 | 6.84±0.90 | 0.179±0.066 | 0.217±0.058 |
| Sham operated rats | 8 | 6.88±0.81 | 0.186±0.038 | 0.223±0.057 |
| Model group | 7 | 9.36±0.94■■▲▲ | 0.293±0.062■■▲▲ | 0.664±0.080■■▲▲ |
| Donepezil group | 8 | 7.45±0.82●● | 0.212±0.061● | 0.312±0.124 |
| Chinese medicine group | 7 | 7.25±1.56●● | 0.241±0.061 | 0.369±0.063■■▲▲●● |
| Group in Chinese medicine | 8 | 7.71±1.58● | 0.247±0.091 | 0.322±0.111■▲●● |
| Big group of Chinese medicine | 7 | 7.64±1.54● | 0.230±0.123 | 0.316±0.139●● |
Note: compare with normal group,■P ＜ 0.05,■■P ＜ 0.01；Compare with sham operated rats,▲P ＜ 0.05,▲▲P ＜ 0.01；Compare with model group,●P ＜ 0.05,●●P ＜ 0.01；Compare with donepezil group,**P
＜ 0.01.
1.3 ginseng fiber crops Yizhi Decoctions are on SOD, MDA and GSH, the impact of GSH-PX content in rat hippocampus
Compare with normal group, model group superoxide dismutase (SOD), malonaldehyde (MDA) and reduction
Type glutathion (GSH), glutathion-peroxidase (GSH-PX) content all have notable significant difference
(P<0.01)；Rats in sham-operated group is all without notable significant difference (P > 0.05).Compare with model group, many
Donepezil group SOD, MDA and GSH content have notable significant difference (P < 0.05 or P < 0.01)；
Chinese medicine group MDA and GSH content have notable significant difference (P < 0.05 or P < 0.01)；In Chinese medicine
Group SOD, MDA and GSH content has notable significant difference (P < 0.05 or P < 0.01)；Chinese medicine is big
Group SOD, MDA and GSH-PX content has notable significant difference (P < 0.05 or P < 0.01).Result
It is shown in Table 3.
SOD, MDA and GSH, the comparison of GSH-PX content in rat hippocampus respectively organized by table 3.
Note: compare with normal group,■P ＜ 0.05,■■P ＜ 0.01；Compare with sham operated rats,▲P ＜ 0.05,▲▲P ＜ 0.01；Compare with model group,●P ＜ 0.05,●●P ＜ 0.01；Compare with donepezil group,**P ＜
0.01。
The impact that CA1 district Bcl-2 Yu Bax in rat hippocampus is expressed by 1.4 ginseng fiber crops Yizhi Decoctions
Compare with normal group, the model group Hippocampal CA 1 apoptogene B cell lymphoma factor-2 (B cell
Leukemia2, Bcl-2) and the B cell lymphoma factor-2 relatedness X protein (Bcl-2associated X
Protein, Bax) express all have notable significant difference (P < 0.01)；Rats in sham-operated group is all without notable statistics
Learn difference (P > 0.05).Compare with model group, each medication intervention group Hippocampal CA 1 Bcl-2 and Bax table
Reach and all have notable significant difference (P < 0.05 or P < 0.01).The results are shown in Table 4.
The comparison that CA 1 Zone of Hippocampus in Mouse Bcl-2 Yu Bax expresses respectively organized by table 4.
| Group | n | Bcl-2 (%) | Bax (%) |
| Normal group | 8 | 36.98±7.73 | 37.83±5.32 |
| Sham operated rats | 8 | 37.19±8.43 | 35.95±7.96 |
| Model group | 7 | 19.48±3.71■■▲▲ | 60.92±10.7■■▲▲ |
| Donepezil group | 8 | 34.48±9.57●● | 40.73±7.13●● |
| Chinese medicine group | 7 | 32.48±6.21●● | 42.48±9.94●● |
| Group in Chinese medicine | 8 | 31.98±9.52●● | 41.98±9.71●● |
| Big group of Chinese medicine | 7 | 29.51±5.95● | 42.36±10.32●● |
Note: compare with normal group,■P ＜ 0.05,■■P ＜ 0.01；Compare with sham operated rats,▲P ＜ 0.05,▲▲P ＜ 0.01；Compare with model group,●P ＜ 0.05,●●P ＜ 0.01；Compare with donepezil group,**P
＜ 0.01
2. the clinical research of ginseng fiber crops Yizhi Decoction
Diagnostic criteria meets American Psychiatric Association's mental disorder diagnosis handbook and statistic handbook standard (1994
Year, fourth edition, DSM-IV), US National sacred disease and apoplexy institute and Switzerland's Neuroscience Research
Vascular dementia diagnostic criteria that association (NINDS/AIREN) works out and Chinese Medical Association's nerve in 2002
Sick credit can vascular dementia diagnostic criteria.The vascular dementia traditional Chinese medical science worked out in conjunction with Tian Jinzhou in 1997 etc.
Dialectical scale (scale for thedifferentiation of syndromesof vascular dementia, SDSVD)
Carry out vascular dementia TCM syndrome quantitative criteria, draft VD void stasis blocking key, the Syndrome Differentiation of Chinese Medicine of liver-yang upper harassing syndrome
Card draft.
Between in June, 2012 in December, 2015, employing is random, oxiracetam compares, the examination of non-pessimum
Test principle, include experimenter 65 example in, from clinical symptoms, mini-mentalstate examination table (MMSE),
The aspects such as activities of daily living scale (ADL), serum central cholinergic system are studied, and observe
The clinical efficacy of compound of Chinese medicine light moderate Patients with Vascular Dementia and safety, 6 weeks be a course for the treatment of, altogether
Observe 2 courses for the treatment of.(2 example patients take because can not adhere to filter out complete case history 63 parts after terminating the course for the treatment of
Granule comes off) test group (taking ginseng fiber crops Yizhi Decoction) 31 examples, matched group (taking oxiracetam) 32
Example.Two groups of patient's no difference of science of statistics in terms of age, sex, course of disease isobase data, have comparability.
2.1 liang of groups ADL integration, MMSE integral contrasts
Comparing between group, before and after two groups of treatments, ADL integration, MMSE integration are all without notable significant difference
(P>0.05)；Compare in group, two groups treatment after relatively treatment before all have significant difference (P < 0.05 or
P < 0.01), the results are shown in Table 5.
5. liang of table group ADL integration, MMSE integral contrast
Note: compare between group, compares with matched group,△P<0.05,△△P<0.01；Compare in group, before treatment
Relatively,#P<0.05,##P<0.01。
The comparison of 2.2 liang of group MMSE curative effects
For two groups of MMSE projects, all having the trend of increase, only treatment group orientation force integration is relatively
Notable significant difference (P < 0.05) is had before treatment.The results are shown in Table 6.
The comparison of 6. liang of table group MMSE curative effect
Note: with table 5.
Before and after 2.3 liang of group treatments, disease total mark compares
For disease integral contrast before and after two groups of treatments, compare between group, with matched group comparison therapy before without notable
Significant difference (P>0.05), has notable significant difference (P<0.01) after treatment.Compare in group, with
Comparing before treatment, two groups all have notable significant difference (P < 0.01).The results are shown in Table 7.
Before and after the group treatment of 7. liang of table, disease total mark compares
Note: with table 5.
The comparison of 2.4 liang of group Ach, AchE content
On patients serum's acetylcholine (Ach), the impact of acetylcholinesterase (AchE) content, in group with
Matched group compares, and equal no difference of science of statistics (P > 0.05) before treatment, after treatment, only Ach has notable statistics
Difference (P < 0.05).Comparing in group, relatively compare before treatment, Ach, AchE content is all had significantly by two groups
Significant difference (P < 0.01).The results are shown in Table 8.
The comparison of 8. liang of table group Ach, AchE content
Note: with table 5.
Two groups of patients have carried out three big conventional and hepatic and renal function inspections the most afterwards, all do not find that inspection refers to
Target abnormal change.
The explanation of above example is only intended to help to understand the core concept of the present invention.It is right to it should be pointed out that,
For those skilled in the art, under the premise without departing from the principles of the invention, it is also possible to
Medicine preparation to the present invention carries out some improvement and modification, and these improve and modification also falls into present invention power
In the protection domain that profit requires.
Claims (7)
1. the Chinese medicine composition that a treatment of vascular is dull-witted, it is characterised in that include Radix Ginseng, Rhizoma Gastrodiae,
Ramulus Euonymi and Rhizoma Chuanxiong.
The Chinese medicine composition that treatment of vascular the most according to claim 1 is dull-witted, it is characterised in that
Counting by weight, the consumption of each component is Radix Ginseng 6～12 parts, Rhizoma Gastrodiae 6～12 parts, Ramulus Euonymi 6～12
Part, Rhizoma Chuanxiong 3～9 parts.
The Chinese medicine composition that treatment of vascular the most according to claim 2 is dull-witted, it is characterised in that
Counting by weight, the consumption of each component is Radix Ginseng 9 parts, 9 parts of Rhizoma Gastrodiae, Ramulus Euonymi 9 parts, Rhizoma Chuanxiong 6
Part.
4. the Chinese medicine preparation crude drug that a treatment of vascular is dull-witted, it is characterised in that use claim
1～3 arbitrary described Chinese medicine compositions, are prepared by the following method:
Radix Ginseng and Rhizoma Chuanxiong mixing, add 70% (v/v) ethanol water reflux, extract, of 8 times of weight, collect leaching
Go out liquid, in Radix Ginseng and Rhizoma Chuanxiong, add 70% (v/v) ethanol water reflux, extract, of 4 times of weight the most again,
Collecting leachate, the mixing of two-stage leaching liquid concentrates, and obtains alcohol extraction thick paste；
Rhizoma Gastrodiae and Ramulus Euonymi mixing, add the soak by water of 10 times of weight, filter, add the water of 8 times of weight
Continue to decoct, filter, merge the filtrate of twice, obtain water after concentrating under reduced pressure and carry thick paste；
Alcohol extraction thick paste and water carry thick paste merge, vacuum drying, pulverize.
The Chinese medicine preparation crude drug that treatment of vascular the most according to claim 4 is dull-witted, its feature exists
In, Radix Ginseng and Rhizoma Chuanxiong return time for the first time is 2.0 hours, and return time is 1.5 hours for the second time.
The Chinese medicine preparation crude drug that treatment of vascular the most according to claim 4 is dull-witted, its feature exists
In, Rhizoma Gastrodiae and Ramulus Euonymi decocting time for the first time is 2.0 hours, and decocting time is 1.5 hours for the second time.
The Chinese medicine preparation crude drug that treatment of vascular the most according to claim 4 is dull-witted, its feature exists
In, the temperature conditions of described concentrating under reduced pressure is 60 DEG C.
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|---|---|---|---|---|
| CN1695715A (en) * | 2005-05-17 | 2005-11-16 | 刘升胜 | Medication for treating dementia in blood vessel type, and preparation method |
| CN1742968A (en) * | 2005-09-26 | 2006-03-08 | 山东省千佛山医院 | Medicine for treating vascular dementia and preparing method |
| CN101791337A (en) * | 2010-03-26 | 2010-08-04 | 苏州卫生职业技术学院 | Composition for treatment of vascular dementia and preparation method thereof |
| CN101954031A (en) * | 2010-10-07 | 2011-01-26 | 山东省千佛山医院 | Medicament for treating vascular dementia and preparation method |
| CN102068530A (en) * | 2011-01-21 | 2011-05-25 | 山东省中医药研究院 | Traditional Chinese medicine for preventing and treating vascular dementia |
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2016
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1695715A (en) * | 2005-05-17 | 2005-11-16 | 刘升胜 | Medication for treating dementia in blood vessel type, and preparation method |
| CN1742968A (en) * | 2005-09-26 | 2006-03-08 | 山东省千佛山医院 | Medicine for treating vascular dementia and preparing method |
| CN101791337A (en) * | 2010-03-26 | 2010-08-04 | 苏州卫生职业技术学院 | Composition for treatment of vascular dementia and preparation method thereof |
| CN101954031A (en) * | 2010-10-07 | 2011-01-26 | 山东省千佛山医院 | Medicament for treating vascular dementia and preparation method |
| CN102068530A (en) * | 2011-01-21 | 2011-05-25 | 山东省中医药研究院 | Traditional Chinese medicine for preventing and treating vascular dementia |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110122869A (en) * | 2019-06-25 | 2019-08-16 | 吉林大学珠海学院 | A kind of prevention and treatment Parkinson's disease edible composition |
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