CN105974108B - Immunochromatography detecting system and detection method - Google Patents
Immunochromatography detecting system and detection method Download PDFInfo
- Publication number
- CN105974108B CN105974108B CN201610301443.3A CN201610301443A CN105974108B CN 105974108 B CN105974108 B CN 105974108B CN 201610301443 A CN201610301443 A CN 201610301443A CN 105974108 B CN105974108 B CN 105974108B
- Authority
- CN
- China
- Prior art keywords
- detection
- color lump
- light source
- photodetector
- repair
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/8483—Investigating reagent band
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Theoretical Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Mathematical Physics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Spectrometry And Color Measurement (AREA)
Abstract
A kind of immunochromatography detecting system, including：Detect light source, standard sources, photodetector, Test paper；The Test paper sets detection zone, and detection zone sets detection line, when, there are during target detection thing, nano gold mark composition granule therein occurs immunochromatography reaction and is gathered in detection line in sample；Detection light and reflected light can be launched to Test paper respectively by detecting light source and standard sources；Photodetector can receive the reflected light of the detection zone；Immunity chromatography detection test paper sets calibration region, calibration region sets calibration color lump, calibration color lump is located at the color range of the nano gold mark composition granule, and the detection irradiated by standard light and detection light for calibration color lump is modified for testing result and determines the need for further correcting.
Description
Technical field
The present invention relates to immuno-chromatographic assay technology field, more particularly, to immunochromatography detecting system.
Background technology
Colloidal gold solution refers to aurosol of the dispersed phase particles diameter between l -150nm, belongs to multiphase heterogeneous body
System, color is in salmon pink to aubergine.Colloidal gold can be used as probe to carry out cell surface and intracellular polysaccharide, protein, more
The large biological molecules such as peptide, antigen, hormone, nucleic acid are accurately positioned, and can be used for daily immunodiagnosis.Since colloidal gold is made
Have many good qualities for label, therefore from having obtained rapid hair in many research fields at home and abroad since the technology comes out
Exhibition.It has been widely used in Electronic Speculum, flow cytometer, Western blotting, protein staining, the field such as manufacture of external diagnosis reagent.Mesh
The preceding application in medical test is mainly immunochromatographic method.
Gold-marking immunity chromatographic technique (gold-labeled immunochromatography assay, GICA) is a kind of
The solid phase labelling that the multiple technologies such as colloidal gold-labeled method, immunoassay technology and Chromatographic techniques are combined together is immunized
Detection technique.Because its is easy to operate, quick, and there is very high specificity and sensitiveness, in recent years in clinical diagnosis, bedside
The application development of context of detection is rapid, is widely applied in body fluid detection.
Gold-marking immunity chromatographs detecting system application colloidal gold technique and antibody, antigentic specificity rule are detected,
Work as test solution（Different according to the project of inspection, sample can use patients serum, saliva or urine）When instilling test paper, test solution passes through capillary
Effect, colloidal gold is taken on the p-wire for being adsorbed with antibody, if the antigen in test solution is corresponding with this antibody, is produced special
Property combine, colloidal gold is trapped on p-wire, in particular color.Using with light source and photodetector to Test paper into
The instant interpretation of row, can realize and target checking matter is quantitatively detected.
Conventional technique scheme, forms irradiation light on golden immunity chromatography detection test paper surface by LED light source, uses photoelectricity
Diode array receives the scattering light of golden immunity chromatography detection test paper, by the quantitative measurment to nanogold particle, realizes to mesh
Mark the Quantitative detection of gold label test strip.But shadow caused by system unit aging is not accounted in conventional technique scheme
Ring, in order to overcome the drawbacks described above of the prior art, 201210185414.7 patent of invention proposes a kind of gold-marking immunity chromatography
The performance correction method of ELISA test strip system, the data message of colloidal-gold strip colorimetric card when being dispatched from the factory by detecting system, according to this
Data message is established and the corresponding optimal response curve of detectable substance concentration；In the specific time cycle, colorimetric card is detected again
Corresponding data message, by it compared with calibration standard, so as to calculate correction factor, passes through the correction factor amendment system
The result for components aging of uniting.But also there are following defect for the technical solution of the invention：On the one hand, it needs to be spaced specific week
Phase can be modified, can not real-time judge whether need to be modified；On the other hand, it is only value revision that it, which is corrected,
Without considering specific physical process, the result is that nonlinear, accuracy deficiency.
The content of the invention
The present invention provides improved technical solution, can solve the above problem of the prior art, and improves modified
Accuracy.
As one aspect of the present invention, there is provided a kind of immunochromatography detecting system, including：Light source is detected, photoelectricity is visited
Survey device, immunity chromatography detection test paper；The immunity chromatography detection test paper sets detection zone, and the detection zone sets detection
Line, when, there are during target detection thing, nano gold mark composition granule therein occurs immunochromatography reaction and is gathered in detection in sample
On line；The detection light source can launch detection light to the detection zone；The photodetector can receive the detection
The reflected light in region；It is characterized in that：Standard sources is further included, it can launch standard light to the detection zone；It is described to exempt from
Epidemic disease chromatography detecting test paper sets calibration region, and the calibration region sets calibration color lump, and the calibration color lump is located at the nanometer
The color range of gold mark composition granule, by standard light and detection light for the detection that calibration color lump irradiates for testing result into
Row is corrected and determines the need for further correcting.
Preferably, the standard sources is to be capable of providing the light source of stable emergent light intensity, it is arranged to far below detection
The correction of light source and replacement cycle.
Preferably, colorimetric card is further included, it identifies target detection thing concentration and the relation of color gradient.
Preferably, memory module, it stores colorimetric card colourity and the corresponding optimal response of photo detector signal intensity
Curve.
Preferably, the calibration color lump includes identical the first color lump and the second color lump of color, the first color lump table
Face covers the photic zone of specific light transmittance, and the second color lump surface does not cover photic zone.
Preferably, the euphotic light transmittance is 70%~90%.
Preferably, the euphotic light transmittance is 80%.
Preferably, the calibration color lump further includes the 3rd color lump and the 4th color lump, the 3rd color lump and the 4th color
Block is respectively different colours, wherein lower limit of the 3rd color lump color close to the color range of the nano gold mark composition granule, the
The upper limit of the four color lump colors close to the color range of the nano gold mark composition granule.
As another aspect of the present invention, there is provided the detection method of immunochromatography detecting system, includes the following steps：
（1）The calibration color lump in calibration region is irradiated by standard sources and detection light source respectively, it is true according to the response of photodetector
Regular inspection surveys the correction factor of light source；（2）Determine the need for carrying out photodetector sound according to the modified result of detection light source
Answer the amendment of coefficient；Entered step if necessary to correct（3）Entered step if need not correct（4）；（3）Visited for photoelectricity
Device response coefficient is surveyed to be modified；（4）By detecting light source irradiating and detecting region, revised signal strength is obtained；（5）According to
Revised signal strength, determines the target detection thing concentration of sample.
Preferably, the step（1）In, respectively first color lump and the are irradiated by detecting light source and standard sources
Two color lumps, according to photodetector in response to determining that the amendment of detection light source；The amendment includes the following steps：1st, basisCalculate correction factor k and b, wherein LMark 1Photodetector is examined during to irradiate the first color lump using standard sources
The signal strength of survey, LMark 2The signal strength that photodetector detects during to irradiate the second color lump using standard sources；LInspection 1To use
Detect the signal strength of photodetector detection when light source irradiates the first color lump, LInspection 2To irradiate the second color lump using detection light source
When photodetector detection signal strength；2nd, when detecting light source irradiating and detecting region, the corresponding photodetection of detection light source
The signal strength of device detection is modified to LRepair=kLSurvey+b。
Preferably, the step（2）In, by optimal response curve, calculate the expectation of the 3rd color lump and the 4th color lump
Signal strength, LPhase 3=k1X1+b1And LPhase 4=k1X2+b1, wherein k1 and b1 are optimal response slope of a curve and intercept, X1 and X2
The respectively color range gradient magnitude of the 3rd color lump and the 4th color lump；The 3rd color lump and the 4th color lump are measured by detecting light source
Signal strength LSurvey 3And LSurvey 4, revised signal strength L is calculated respectivelyRepair 3=kLSurvey 3+ b and LRepair 4=kLSurvey 4+ b, if LPhase 3- LRepair 3With
LPhase 4- LRepair 4In the absolute value of any one be more than specific threshold, carry out the amendment of further photodetector response coefficient.
Preferably, the step（3）The correction value of middle photodetector response coefficient calculates as follows:(a) make
The data message of each gradient region of colorimetric card is obtained successively as light source, photodetector by the use of detection light source；（b）It is detected
The amendment of light source, obtains the revised signal strength L of each gradient regionRepair i；（c）By optimal response curve, calculate respectively each
The expectation signal strength L of a gradient regionPhase i；（d）Calculate the correction value ε of the response coefficient of each gradient regioni=LPhase i/LRepair i。
Preferably, in the step (4), the signal strength of the corresponding photodetector detection of detection light source is modified to LRepair=
kLSurvey+b；According to step（2）Result judgement whether need the amendment of photodetector response coefficient, it is if necessary to correct, then right
In LRepairThe amendment step of photodetector response coefficient be：（a1）Obtain the response coefficient that its colourity corresponds to gradient region endpoint
Correction value ε1And ε2；（a2）By for ε1And ε2Carry out the correction value ε that interpolation calculation goes out the colourityRepair；（a3）Calculate the signal
Intensity LRepairFinal correct Lf=LRepair ×εRepair。
Brief description of the drawings
Fig. 1 is the immunochromatography detecting system schematic diagram of the embodiment of the present invention.
Fig. 2 is the overhaul flow chart of the immunochromatography detecting system of the embodiment of the present invention.
Embodiment
In order to illustrate more clearly of technical scheme, embodiment will be used simply to be situated between the present invention below
Continue, it should be apparent that, in describing below is only one embodiment of the present of invention, is come for those of ordinary skill in the art
Say, without having to pay creative labor, other technical solutions can also be obtained according to these embodiments, are fallen within
Disclosure of the invention scope.
The immunochromatography detecting system of the embodiment of the present invention, referring to Fig. 1, including immunity chromatography detection test paper 10, detection light
Source 20, standard sources 30 and photodetector 40.
Detect light source 20 to be used to provide incident light to immunity chromatography detection test paper 10, received by using photodetector 40
The scattering light of immunity chromatography detection test paper 10, can use such as LED light source as detection light source.Standard sources 30 is emergent light
Intensity keeps stable light source, it is arranged to correction and replacement cycle far below detection light source 20, single 30 energy of standard sources
It is enough in multiple detecting systems.
Immunity chromatography detection test paper 10 includes filling opening 1, detection zone 2 and calibration region 3.Filling opening 1 sets water suction
Pad, for adding detection sample to test paper by filling opening 1, detection sample can be body fluids such as blood etc..Set in detection zone 2
Detection line is put, when, there are during target detection thing, sample takes absorption to through capillary action, by nano gold mark composition granule in sample
Have on the p-wire of antibody, if the antigen in sample is corresponding with this antibody, produce specific binding, colloidal gold is trapped in survey
Try on line, in particular color.The light sent by detecting light source is radiated at test strips fore-telling, and test strips surface scattering light is visited by photoelectricity
Device is surveyed to receive.Luminous intensity reflects degree of absorption of the object to incident light, according to Lambert-Beer's law, luminous intensity and mesh in sample
It is linear to mark the concentration of checking matter, so according to the measurement result of photodetector, can determine detected material in sample
Concentration.
The calibration region 3 of immunity chromatography detection test paper is used for the aging effect of detecting system, including the first color lump 31, second
Color lump the 32, the 3rd calibrates color lump 33 and the 4th and calibrates color lump 34.Wherein the first color lump 31 and the second color lump 32 have identical
Color, for carrying out determining for Light source correction coefficient.First color lump, 31 surface covers specific light transmittance such as 80% light transmittance
Photic zone, 32 surface of the second color lump do not cover photic zone.
3rd color lump 33 and the 4th color lump 34 are used for the degree of aging for judging photodetector, determine whether to need
Carry out the aging amendment of photodetector.3rd color lump 33 and the 4th color lump 34 are respectively different colours, wherein the 3rd color lump
33 colors are close to the lower limit of the color range of nano gold mark composition granule, and 34 color of the 4th color lump is close to nano gold mark composition granule
Color range the upper limit.
Immunochromatography detecting system further includes colorimetric card and memory module, and wherein colorimetric card is dense for identifying target detection thing
The relation of degree and color gradient, memory module is used to store colorimetric card colourity and photo detector signal intensity is corresponding optimal
Response curve.Wherein, optimal response curve negotiating following manner determines：Detected when system is dispatched from the factory by photodetectors register
The data message of colorimetric card, it is corresponding optimal with photo detector signal intensity to establish colorimetric card colourity according to this data message
Response curve.
Technical scheme is based on following principle, and the aging of detecting system includes aging and the photoelectricity of light-source system
The aging of detector.The aging of wherein light-source system is due to that the luminescent properties of light source such as light emitting diode are degenerated, and is caused same
The input parameter of sample, its luminous intensity weaken, and thus cause the error of testing result, its caused error is linear.And light
During electric explorer such as photodiode aging, the change of its photoelectric response performance, causes the response coefficient change for colourity light source
Change, error caused by the change is nonlinear.In the prior art such as 201210185414.7 patents of invention, by using single
Correction factor the aging of light-source system and the aging of photodetector are subjected to simple value revision together, can not react
The physical process of aging, causes correction result undesirable.Also, the digestion period of light source and photodetector be also it is different,
The possible light source ages cycle is shorter and the digestion period of photodetector is longer, it is only necessary to which the aging into line light source is corrected.
Referring to Fig. 2, the detection method of the immunochromatography detecting system of the embodiment of the present invention, includes the following steps：（1）Respectively
The calibration color lump in calibration region 3 is irradiated by standard sources 30 and detection light source 20, it is true according to the response of photodetector 40
Regular inspection surveys the correction factor of light source 20；（2）Determine the need for carrying out photodetection according to the modified result of detection light source 20
The amendment of 40 response coefficient of device；Entered step if necessary to correct（3）Entered step if need not correct（4）；（3）For
40 response coefficient of photodetector is modified；（4）By detecting 20 irradiating and detecting region 2 of light source, revised signal is obtained
Intensity；（5）According to revised signal strength, the target detection thing concentration of sample is determined.
The step（1）In, respectively the first color lump 31 and the second color are irradiated by detecting light source 20 and standard sources 30
Block 32, according to photodetector 40 in response to determining that the amendment of detection light source 20；The amendment includes the following steps：1st, basisCalculate correction factor k and b, wherein LMark 1Photodetection during to irradiate the first color lump 31 using standard sources 30
The signal strength that device 40 detects, LMark 2The signal that photodetector 40 detects during to irradiate the second color lump 32 using standard sources 30
Intensity；LInspection 1The signal strength that photodetector 40 detects during to irradiate the first color lump 31 using detection light source 20, LInspection 2To use inspection
Survey the signal strength that photodetector 40 detects when light source 20 irradiates the second color lump 32；2nd, in 20 irradiating and detecting region of detection light source
When 2, the signal strength that the corresponding photodetector 40 of detection light source 20 detects is modified to LRepair=kLSurvey+b。
The step（2）In, by optimal response curve, the expectation signal of the 3rd color lump 33 of calculating and the 4th color lump 34
Intensity, LPhase 3=k1X1+b1And LPhase 4=k1X2+b1, wherein k1 and b1 are optimal response slope of a curve and intercept, and X1 and X2 distinguish
For the 3rd color lump 33 and the color range gradient magnitude of the 4th color lump 34；The 3rd color lump 33 and the 4th color are measured by detecting light source 20
The signal strength L of block 34Survey 3And LSurvey 4, revised signal strength L is calculated respectivelyRepair 3=kLSurvey 3+ b and LRepair 4=kLSurvey 4+ b, if
LPhase 3- LRepair 3And LPhase 4- LRepair 4In the absolute value of any one be more than specific threshold, carry out further photodetector 40 and respond
The amendment of coefficient.
The step（3）The correction value of middle 40 response coefficient of photodetector calculates as follows:(a) using detection
Light source 20 is used as light source, and photodetector 40 obtains the data message of each gradient region of colorimetric card successively；（b）It is detected light
The amendment in source 20, obtains the revised signal strength L of each gradient regionRepair i；（c）By optimal response curve, calculate respectively each
The expectation signal strength L of a gradient regionPhase i；（d）Calculate the correction value ε of the response coefficient of each gradient regioni=LPhase i/LRepair i。
In the step (4), the signal strength that the corresponding photodetector 40 of detection light source 20 detects is modified to LRepair=kLSurvey
+b；According to step（2）Result judgement whether need the amendment of 40 response coefficient of photodetector, it is if necessary to correct, then right
In LRepairThe amendment step of 40 response coefficient of photodetector be：（a1）Obtain the response system that its colourity corresponds to gradient region endpoint
Several correction value ε1And ε2；（a2）By for ε1And ε2Carry out the correction value ε that interpolation calculation goes out the colourityRepair；（a3）Calculate the letter
Number intensity LRepairFinal correct Lf=LRepair ×εRepair。
After light source modification and the amendment of photodetector response coefficient has been carried out, light source modification and photodetection are obtained
The revised signal strength of device response coefficient, according to the correspondence of signal strength and target detection thing concentration, can determine sample
The concentration of target detection thing in product.
All documents that the present invention refers to all incorporated by reference in this application, work is individually recited just as each document
For with reference to such.In addition, it should also be understood that, after the above disclosure of the present invention has been read, protection scope of the present invention and not only
Above-described embodiment is confined to, those skilled in the art can make various modifications or changes to the present invention, is not departing from original of the invention
Under the premise of reason, these equivalent forms also fall within the scope of the appended claims of the present application.
Claims (1)
1. a kind of detection method for the immunochromatography detecting system for being used to determine the concentration of target detection thing in sample, described immune
Chromatography detecting system includes：Detect light source, photodetector, immunity chromatography detection test paper；The immunity chromatography detection test paper is set
Put detection zone, the detection zone sets detection line, when in sample there are during target detection thing, nano gold mark thing therein
Particle occurs immunochromatography reaction and is gathered in detection line；The detection light source can launch detection light to the detection zone；
The photodetector can receive the reflected light of the detection zone；It is characterized in that：Immunochromatography detecting system further includes
Standard sources, it can launch standard light；The standard sources is to be capable of providing the light source of stable emergent light intensity, it is arranged to
Correction and replacement cycle far below detection light source；Memory module, it stores colorimetric card colourity and photo detector signal intensity
Corresponding optimal response curve；
The immunity chromatography detection test paper sets calibration region, and the calibration region sets calibration color lump, the calibration color lump position
In the color range of the nano gold mark composition granule；The calibration color lump includes the first identical color lump of color, the second color lump
And different the 3rd color lump of color, the 4th color lump；The first color lump surface covers the photic zone of specific light transmittance, and described the
Two color lump surfaces do not cover photic zone；The lower limit of color range of the 3rd color lump color close to nano gold mark composition granule,
The upper limit of color range of the 4th color lump color close to nano gold mark composition granule；By standard light and detection light for school
The detection of quasi- color lump irradiation is modified for testing result and determines the need for further correcting；
Wherein, first color lump and the second color lump are irradiated by detecting light source and standard sources respectively, according to photodetection
Device in response to determining that detection light source amendment；The amendment includes the following steps：（1）According toCalculate and correct system
Number k and b, wherein LMark 1The signal strength that photodetector detects during to irradiate the first color lump using standard sources, LMark 2To use mark
The signal strength of photodetector detection when the second color lump is irradiated in quasi-optical source；LInspection 1During to irradiate the first color lump using detection light source
The signal strength of photodetector detection, LInspection 2The signal that photodetector detects during to irradiate the second color lump using detection light source
Intensity；（2）When detecting light source irradiating and detecting region, the signal strength of the corresponding photodetector detection of detection light source is modified to
LRepair=kLSurvey+b；Determine the need for carrying out the amendment of photodetector response coefficient according to the modified result of detection light source：It is logical
Optimal response curve is crossed, calculates the expectation signal strength of the 3rd color lump 33 and the 4th color lump 34, LPhase 3=k1X1+b1And LPhase 4=
k1X2+b1, wherein k1 and b1 are optimal response slope of a curve and intercept, and X1 and X2 are respectively the 3rd color lump 33 and the 4th color lump
34 color range gradient magnitude；The signal strength L of the 3rd color lump 33 and the 4th color lump 34 is measured by detecting light source 20Survey 3And LSurvey 4,
Revised signal strength L is calculated respectivelyRepair 3=kLSurvey 3+ b and LRepair 4=kLSurvey 4+ b, if LPhase 3- LRepair 3And LPhase 4- LRepair 4In it is any one
A absolute value is more than specific threshold, carries out the amendment of further photodetector response coefficient；
（3）The correction value of photodetector response coefficient calculates as follows:(a) detection light source is used as light source, light
Electric explorer obtains the data message of each gradient region of colorimetric card successively；（b）The amendment of light source is detected, obtains each ladder
Spend the revised signal strength L in regionRepair i；（c）By optimal response curve, the expectation signal of each gradient region is calculated respectively
Intensity LPhase i；（d）Calculate the correction value ε of the response coefficient of each gradient regioni=LPhase i/LRepair i；
（4）According to step（2）Result judgement whether need the amendment of photodetector response coefficient, if necessary to correct, then
For LRepairThe amendment step of photodetector response coefficient be：For LRepairPhotodetector response coefficient amendment step
For：（a1）Obtain the correction value ε that its colourity corresponds to the response coefficient of gradient region endpoint1And ε2；（a2）By for ε1And ε2Into
Row interpolation calculates the correction value ε of the colourityRepair；（a3）Calculate signal strength LRepairFinal correct Lf=LRepair ×εRepair。
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610301443.3A CN105974108B (en) | 2016-05-07 | 2016-05-07 | Immunochromatography detecting system and detection method |
| CN201711027305.1A CN107525926B (en) | 2016-05-07 | 2016-05-07 | Immunochromatography detection method and detection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610301443.3A CN105974108B (en) | 2016-05-07 | 2016-05-07 | Immunochromatography detecting system and detection method |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711027305.1A Division CN107525926B (en) | 2016-05-07 | 2016-05-07 | Immunochromatography detection method and detection system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105974108A CN105974108A (en) | 2016-09-28 |
| CN105974108B true CN105974108B (en) | 2018-05-01 |
Family
ID=56992121
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610301443.3A Active CN105974108B (en) | 2016-05-07 | 2016-05-07 | Immunochromatography detecting system and detection method |
| CN201711027305.1A Active CN107525926B (en) | 2016-05-07 | 2016-05-07 | Immunochromatography detection method and detection system |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711027305.1A Active CN107525926B (en) | 2016-05-07 | 2016-05-07 | Immunochromatography detection method and detection system |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN105974108B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110346551A (en) * | 2018-04-04 | 2019-10-18 | 南京东纳生物科技有限公司 | A kind of fluorescence immune chromatography quantitative analysis instrument testing calibration card and its detection method |
| CN110579598B (en) * | 2018-06-07 | 2023-12-05 | 昆明联恩生物技术有限责任公司 | Optical signal detection device, system and method |
| CN117074345B (en) * | 2023-10-16 | 2024-01-16 | 山东风途物联网科技有限公司 | Detection and calibration method for optical equipment for water quality detection |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102426162A (en) * | 2010-12-31 | 2012-04-25 | 广州万孚生物技术有限公司 | Fluorescence quantitative detector |
| CN102279263B (en) * | 2011-05-06 | 2014-06-18 | 杭州顿力医疗器械股份有限公司 | CCD-type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers |
| CN103513029A (en) * | 2012-06-18 | 2014-01-15 | 王胜 | Immunochromatographic analyzer calibration method |
| CN103063834A (en) * | 2012-12-28 | 2013-04-24 | 三诺生物传感股份有限公司 | Method and system for analysis of immune quantitative chromatographic assay strip |
| CN103063835B (en) * | 2013-01-05 | 2014-12-31 | 福州大学 | Correction test strip for immunochromatographic test strip quantitative detecting instrument, and manufacturing method thereof |
| KR101562946B1 (en) * | 2013-04-23 | 2015-10-26 | 주식회사 수젠텍 | Devices and Methods for Detecting Analytes in Samples |
| CN104297468B (en) * | 2013-07-15 | 2016-04-20 | 艾博生物医药(杭州)有限公司 | The calibration steps of a kind of immune fetch equipment and this equipment |
-
2016
- 2016-05-07 CN CN201610301443.3A patent/CN105974108B/en active Active
- 2016-05-07 CN CN201711027305.1A patent/CN107525926B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN107525926A (en) | 2017-12-29 |
| CN107525926B (en) | 2020-03-27 |
| CN105974108A (en) | 2016-09-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1242270C (en) | Biosensors and measurement method | |
| CN102707051B (en) | Performance correction method for gold mark immunochromatographic test strip detection system | |
| JPWO2007007849A1 (en) | Analysis apparatus and analysis method | |
| CN101825640A (en) | Qualitative and quantitative detection dual-purpose HCG test paper for colloidal gold immunochromatography assay | |
| CN207248894U (en) | Bladder chalone C time resolution detection card and kit | |
| CN105974108B (en) | Immunochromatography detecting system and detection method | |
| CN105510577A (en) | Method for rapidly and quantitatively detecting multiple analytes in blood by adopting multi-point calibration | |
| CN105388283A (en) | Immunofluorescence colored tape quantitative determination system and application thereof | |
| CN104297468B (en) | The calibration steps of a kind of immune fetch equipment and this equipment | |
| CN103063834A (en) | Method and system for analysis of immune quantitative chromatographic assay strip | |
| KR101718485B1 (en) | Device for Detecting Colored Reaction or Fluorescence Reaction of Immunochromatography | |
| CN101545864A (en) | Optical analysis reading device | |
| CN105866410A (en) | Immunochromatographic detection test strip for joint detection of NSE and CEA, and preparation method and application method thereof | |
| CN201210144Y (en) | Optical analysis reading device | |
| CN106053792B (en) | Immunity chromatography detection test paper and kit | |
| CN103954753A (en) | Quantitative determination method of immune chromatography test strip | |
| US20070122914A1 (en) | Obtaining measurements of light transmitted through an assay test strip | |
| JPWO2003100425A1 (en) | Immunological chromatographic test strip reading and quantifying device | |
| JP2010032396A (en) | Biosensor | |
| CN204287198U (en) | Omnidistance C reactive protein immunochromatographiassay assay quantitative detection test paper | |
| KR20150020804A (en) | Light-permeable diagnostic kit and reader having the same | |
| KR20150029290A (en) | Reading device of diagnostic strip | |
| JP2009031097A (en) | Method of quantifying/detecting measuring objective substance using fine particles and measuring device used for the method | |
| CN104181295B (en) | A kind of multi-thread colloidal gold strip quantitative test image processing method | |
| CN109239338A (en) | Wide-range immuno-chromatography detection device and method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Dong Hui Inventor after: Jin Weirong Inventor after: Qin Hongyou Inventor before: Jiang Cuizhen |
|
| CB03 | Change of inventor or designer information | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20180403 Address after: 210048 Jiangsu Province, Nanjing Jiangbei District Changlu Street six Ning Road No. 606 building 101 room A Applicant after: Nanjing shen you Biotechnology Co., Ltd. Address before: Shijiazhuang City, Hebei Province, the new bridge 050090 West Road No. 369 Applicant before: Jiang Cuizhen |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |