CN105962361A - Preparation method of dicotyledoneae fermentation liquid - Google Patents
Preparation method of dicotyledoneae fermentation liquid Download PDFInfo
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- CN105962361A CN105962361A CN201610309299.8A CN201610309299A CN105962361A CN 105962361 A CN105962361 A CN 105962361A CN 201610309299 A CN201610309299 A CN 201610309299A CN 105962361 A CN105962361 A CN 105962361A
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- eurycoma longifolia
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- 230000004151 fermentation Effects 0.000 title claims abstract description 64
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- 238000002360 preparation method Methods 0.000 title claims abstract description 39
- 241001233957 eudicotyledons Species 0.000 title abstract 5
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- 235000013305 food Nutrition 0.000 claims abstract description 57
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 50
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to preparation method of a dicotyledoneae fermentation liquid. The preparation method comprises the following steps: (1) preparing a food enzymolysis solution; (2) preparing a dicotyledoneae extracting solution; (3) preparing a dicotyledoneae nutrient solution; (4), fermenting; (5) emulsifying. Dicotyledoneae, moringa oleifera powder and various materials with homology of medicine and food are collocated in a reasonable proportion and are fully mixed, and lactobacillus is added for fermentation; a single bacterium strain, namely the lactobacillus, is adopted in the fermentation process, and the specially-made culture solution is taken as a hotbed, so that the fermentation efficiency is exerted more fully; a sodium chloride and acetic acid combination serves as a buffer solution, so that the pH value of the solution is kept within a precontrol range during the fermentation process.
Description
Technical field
The invention belongs to health protection medicine field, relate to fermentation technique, the preparation method of a kind of Eurycoma longifolia fermentation liquid.
Background technology
Eurycoma longifolia has the title of Malaysia Radix Ginseng, be always taken as postnatal nourishing product, treatment diabetes, hypertension, prostatitis,
Pulmonary tuberculosis, fever, jaundice and dysentery.Eurycoma longifolia contains multiple alkaloid, moreover it is possible to can treat malaria, anaphylaxis, send out
The diseases such as burning, it addition, it also contain multiple element can anticancer, antioxidation, rheumatism etc., be suitable for men and women, old and young's each age
The personage of layer.
Eurycoma longifolia is the most only used to medicine or medicated diet, and applicant does not the most find to use with the form of fermentation liquid
Report and technical literature in daily life.
Summary of the invention
It is an object of the invention to overcome prior art not enough, it is provided that a kind of improve non-oxidizability, resisting fatigue Eurycoma longifolia send out
The preparation method of ferment liquid.
The technical solution used in the present invention is:
The preparation method of a kind of Eurycoma longifolia fermentation liquid, comprises the steps:
Preparation food enzymolysis solution: by etc. the purple rice of weight, Semen setariae, Semen Coicis, Poria, Fructus Jujubae, Fructus Mume, Pericarpium Citri Reticulatae, Fructus Crataegi (parched to brown)
Soak, by food: water is the weight ratio mixing of 1:7, the red bean powder of the 1.5% of the gross weight of addition food and water and material gross weight
1.5% 0.05%NaHCO3, after 80 DEG C of 15min that precook, add and adjust the total of pH value 5.5, addition food and water with citric acid
The 0.02% pectase 55 DEG C insulation enzymolysis 2h of weight, adds 1% hemicellulase of the gross weight of food and water, adds food
With 0.56% cellulase of the gross weight of water, add 0.5% papain of the gross weight of food and water, add food and water
0.04% phytase 1h of gross weight, mix Fructus Hordei Germinatus, Massa Medicata Fermentata 15h, filter, sterilization, obtain food enzymolysis solution, stand-by；
(2) preparation Eurycoma longifolia extracting solution: by Eurycoma longifolia water blancing 2min, cooling, by material: water is that the weight ratio of 1:10 adds
Water is pulled an oar, and then mixes with moringa powder, Fructus Amomi Rotundus, Fructus Amomi, Rhizoma Polygonati Odorati, Semen Raphani, adds water to 2.5 times, decocts, and extracts supernatant
Liquid, obtains Eurycoma longifolia extracting solution, stand-by；
(3) the preparation of Eurycoma longifolia nutritional solution: by food enzymolysis solution with Eurycoma longifolia extracting solution by weight the proportions for 4:1
It is mixed, mixed liquor is put in autoclave, be heated to 90 DEG C, and keep 5min, Eurycoma longifolia nutritional solution can be obtained；
(4) fermentation: adding culture fluid in Eurycoma longifolia nutritional solution, Eurycoma longifolia nutritional solution and culture fluid weight ratio are 10:1, add
Enter lactic acid bacteria, sodium chloride and acetic acid mixed fermentation 1day, add the breast of above-mentioned lactic acid bacteria, sodium chloride and acetic acid each half weight
Acid bacterium, sodium chloride and acetic fermentation, obtain fermentation liquid；
(5) emulsifying: adding emulsifying agent in fermentation liquid, cross leaching clear liquid, after pasteurization, packaging gets product, emulsifying agent is food
With gelatin or carboxymethyl cellulose.
And, the content of the solid content of the food enzymolysis solution that step is (1) described is 8%～10%.
And, step (2) described in the solid content of Eurycoma longifolia extracting solution be 4%～6%.
And, step (2) described in Eurycoma longifolia, moringa powder, Fructus Amomi Rotundus, Fructus Amomi, Rhizoma Polygonati Odorati, Semen Raphani parts by weight respectively
For: 20～25 parts, 5～10 parts, 1～2 part, 1～2 part, 1～2 part, 1～2 part.
And, the (3) described lactic acid bacteria of step, sodium chloride, the parts by weight of total consumption of acetic acid are respectively 100～120 parts, 8～10
Part, acetic acid is 4～6 parts.
And, the preparation method of the culture fluid that step is (3) described is: maltose is soluble in water, puts into compound after standing 20min
Vitamin, the post-heating that stirs boils, and is cooled to after room temperature add Ovum Gallus domesticus album, stirs, stand-by, maltose, water, multiple
Close vitamin, the weight ratio of Ovum Gallus domesticus album is 100:10000:3:10.
Advantage of the present invention and good effect be:
The present invention is by the collocation by rational proportion of the various materials of Eurycoma longifolia, moringa powder and integration of edible and medicinal herbs, first by food pair
Answer enzymolysis, be then sufficiently mixed with extracting solution, add lactic acid bacteria and ferment, and sweat uses single culture, i.e.
Lactic acid bacteria, using special culture fluid as hotbed, its fermentation efficiency plays more abundant, the combination conduct of sodium chloride and acetic acid
Buffer solution ensure that in sweat, solution ph is maintained in the range of pre-control.
In the present invention, the consumption of each component is through test of many times and the most constantly to contrast, it was found that involved in technical scheme
Amount ranges can produce unexpected effect, by effective fermentation, make Eurycoma longifolia, moringa powder and Fructus Amomi Rotundus main
Nutritive effect is doubled, although the consumption of each component and effect thereof are only to embody with word and numerical value, but its operating procedure overcomes
Many technology prejudice of the art, is just improved antioxidation and fatigue resistance and has the east of the function that enhances metabolism
Leather Ali's fermentation liquid.
Detailed description of the invention:
Below by specific embodiment, the invention will be further described, and following example are illustrative, is not determinate,
Protection scope of the present invention can not be limited with this.
Embodiment 1
The preparation method of a kind of Eurycoma longifolia fermentation liquid, comprises the steps:
(1) preparation food enzymolysis solution: the purple rice of weight, Semen setariae, Semen Coicis, Poria, Fructus Jujubae, Fructus Mume, Pericarpium Citri Reticulatae, the Jiaoshan Hill such as just
Short, bristly hair or beard soaks, by food: water is the weight ratio mixing of 1:7, the red bean powder of the 1.5% of the gross weight of addition food and water and material gross weight
The 0.05%NaHCO of the 1.5% of amount3, it is allowed to easily to boil, removes raw meat, except bitter taste, after 80 DEG C of 15min that precook, add and use citric acid
Adjust pH value 5.5, add the 0.02% pectase 55 DEG C insulation enzymolysis 2h of the gross weight of food and water, add food and the gross weight of water
0.56% cellulase of the gross weight of 1% hemicellulase of amount, addition food and water, adds the gross weight of food and water
0.04% phytase 1h of the gross weight of 0.5% papain, addition food and water, mixes Fructus Hordei Germinatus, Massa Medicata Fermentata 15h, filters, kills
Bacterium, stand-by (solid content 8%～10%)；
(2) preparation Eurycoma longifolia extracting solution: by Eurycoma longifolia water blancing 2min, cooling, by material: water is that the weight ratio of 1:10 adds
Water is pulled an oar, and then mixes with moringa powder, Fructus Amomi Rotundus, Fructus Amomi, Rhizoma Polygonati Odorati, Semen Raphani, adds water to 2.5 times of the initial water yield, decocts,
Extraction supernatant, stand-by (solid content 4%～6%), Eurycoma longifolia, moringa powder, Fructus Amomi Rotundus, Fructus Amomi, Rhizoma Polygonati Odorati, the weight of Semen Raphani
Amount is respectively as follows: 2000g, 500g, 100g, 100g, 100g, 100g；
(3) the preparation of Eurycoma longifolia nutritional solution: by food enzymolysis solution with Eurycoma longifolia extracting solution by weight the proportions for 4:1
It is mixed, mixed liquor is put in autoclave, be heated to 90 DEG C, and keep 5min, Eurycoma longifolia nutritional solution can be obtained；
(4) fermentation: adding culture fluid in Eurycoma longifolia nutritional solution, Eurycoma longifolia nutritional solution and culture fluid weight ratio are 10:1, add
Enter lactic acid bacteria, sodium chloride and acetic acid mixed fermentation 1day, add the breast of above-mentioned lactic acid bacteria, sodium chloride and acetic acid each half weight
Acid bacterium, sodium chloride and acetic fermentation, obtain fermentation liquid, lactic acid bacteria, sodium chloride, the weight of total consumption of acetic acid be respectively 120g,
Sodium chloride is 9g, and acetic acid is 4.5g；
The preparation method of culture fluid is: maltose is soluble in water, puts into compound vitamin, after stirring after standing 20min
Heated and boiled, adds Ovum Gallus domesticus album after being cooled to room temperature, stirs, stand-by, maltose, water, compound vitamin, the weight of Ovum Gallus domesticus album
Amount ratio is 100:10000:3:10.
(5) emulsifying: adding emulsifying agent in fermentation liquid, cross leaching clear liquid, after pasteurization, packaging gets product, emulsifying agent is food
With gelatin or carboxymethyl cellulose.
Finished product can cream processed, powder process, be processed further for preparation or the cosmetic material of oral liquid, this course of processing not as
This case inventive point, uses conventional prior art to complete, and in the present invention, each raw material all uses commercial goods.
Step (3) in, after mixed liquor is put into autoclave, add yeast extract 7.5%, dextrinosan 10%-20%,
Citric acid 0.15%-0.25%, sodium citrate 0.05%, L malic acid 0.05%, VC0.03%, it is equipped with food additive: xylose
Alcohol, 0.07%-0.12% xanthan gum 0.5% arabic gum, 0.15% pectin, 0.15% sodium alginate, 0.12%-0.23% carboxymethyl are fine
Dimension element sodium, 0.2%-0.8% fatty acid cane sugar ester emulsifying agent, 0.05% 3 polyglycerin ester, 0.2% Fructus Jujubae essence, beverage flavor agent 0.5%
Ethyl vanillin (bean milk powder essence), food coloring 0.02% phylloxanthin, 1% lecithin (source that Beijing is green, purity is 15%),
Whole or a portion in 0.06% daidzein, black vinegar, polydextrose, nicotinic acid, calcium pantothenate, → allocate, filter →
Sterilizing (mixed liquor being put in autoclave, be heated to 90 DEG C, and keep 5m in) → directly beverage can be administered orally by Eurycoma longifolia,
Then need not step fermentation (4) and step emulsifying (5), above-mentioned percentage ratio is all each material and food enzymolysis solution and Eurycoma longifolia
The percentage ratio of the mixed gross weight of extracting solution.
Embodiment 2
The preparation method of a kind of Eurycoma longifolia fermentation liquid, comprises the steps:
(1) preparation food enzymolysis solution: the purple rice of weight, Semen setariae, Semen Coicis, Poria, Fructus Jujubae, Fructus Mume, Pericarpium Citri Reticulatae, the Jiaoshan Hill such as just
Short, bristly hair or beard soaks, the red bean powder of the 1.5% of the gross weight of addition food and water and the 0.05%NaHCO of the 1.5% of material gross weight3, make
Easily boil, remove raw meat, except bitter taste, after 80 DEG C of 15min that precook, add and adjust pH value 5.5 with citric acid, add the total of food and water
The 0.02% pectase 55 DEG C insulation enzymolysis 2h of weight, adds 1% hemicellulase of the gross weight of food and water, adds food
With 0.56% cellulase of the gross weight of water, add 0.5% papain of the gross weight of food and water, add food and water
0.04% phytase 1h of gross weight, mix Fructus Hordei Germinatus, Massa Medicata Fermentata 15h, filter, sterilization, stand-by (solid content 8%～10%)；
(2) preparation Eurycoma longifolia extracting solution: by Eurycoma longifolia water blancing 2min, cooling, by material: water is that the weight ratio of 1:10 adds
Water is pulled an oar, and then mixes with moringa powder, Fructus Amomi Rotundus, Fructus Amomi, Rhizoma Polygonati Odorati, Semen Raphani, adds water to 2.5 times of the initial water yield, decocts,
Extraction supernatant, stand-by (solid content 4%～6%), Eurycoma longifolia, moringa powder, Fructus Amomi Rotundus, Fructus Amomi, Rhizoma Polygonati Odorati, the weight of Semen Raphani
Amount is respectively as follows: 2500g, 1000g, 150g, 150g, 150g, 150g；
(3) the preparation of Eurycoma longifolia nutritional solution: by food enzymolysis solution with Eurycoma longifolia extracting solution by weight the proportions for 4:1
It is mixed, mixed liquor is put in autoclave, be heated to 90 DEG C, and keep 5min, Eurycoma longifolia nutritional solution can be obtained；
(4) fermentation: adding culture fluid in Eurycoma longifolia nutritional solution, Eurycoma longifolia nutritional solution and culture fluid weight ratio are 10:1, add
Enter lactic acid bacteria, sodium chloride and acetic acid mixed fermentation 1day, add the breast of above-mentioned lactic acid bacteria, sodium chloride and acetic acid each half weight
Acid bacterium, sodium chloride and acetic fermentation, obtain fermentation liquid, lactic acid bacteria, sodium chloride, the weight of total consumption of acetic acid be respectively 120g,
Sodium chloride is 9g, and acetic acid is 6g；
The preparation method of culture fluid is: maltose is soluble in water, puts into compound vitamin, after stirring after standing 20min
Heated and boiled, adds Ovum Gallus domesticus album after being cooled to room temperature, stirs, stand-by, maltose, water, compound vitamin, the weight of Ovum Gallus domesticus album
Amount ratio is 100:10000:3:10.
(5) emulsifying: adding emulsifying agent in fermentation liquid, cross leaching clear liquid, after pasteurization, packaging gets product, emulsifying agent is food
With gelatin or carboxymethyl cellulose.
Finished product can cream processed, powder process, be processed further for preparation or the cosmetic material of oral liquid, this course of processing not as
This case inventive point, uses conventional prior art to complete, and in the present invention, each raw material all uses commercial goods.
Step (3) in, after mixed liquor is put into autoclave, add yeast extract 7.5%, dextrinosan 10%-20%,
Citric acid 0.15%-0.25%, sodium citrate 0.05%, L malic acid 0.05%, VC0.03%, it is equipped with food additive: xylose
Alcohol, 0.07%-0.12% xanthan gum 0.5% arabic gum, 0.15% pectin, 0.15% sodium alginate, 0.12%-0.23% carboxymethyl are fine
Dimension element sodium, 0.2%-0.8% fatty acid cane sugar ester emulsifying agent, 0.05% 3 polyglycerin ester, 0.2% Fructus Jujubae essence, beverage flavor agent 0.5%
Ethyl vanillin (bean milk powder essence), food coloring 0.02% phylloxanthin, 1% lecithin (source that Beijing is green, purity is 15%),
Whole or a portion in 0.06% daidzein, black vinegar, polydextrose, nicotinic acid, calcium pantothenate, → allocate, filter →
Sterilizing (mixed liquor being put in autoclave, be heated to 90 DEG C, and keep 5m in) → directly beverage can be administered orally by Eurycoma longifolia,
Then need not step fermentation (4) and step emulsifying (5), above-mentioned percentage ratio is all each material and food enzymolysis solution and Eurycoma longifolia
The percentage ratio of the mixed gross weight of extracting solution.
Embodiment 3
The preparation method of a kind of Eurycoma longifolia fermentation liquid, comprises the steps:
Preparation food enzymolysis solution: by etc. the purple rice of weight, Semen setariae, Semen Coicis, Poria, Fructus Jujubae, Fructus Mume, Pericarpium Citri Reticulatae, Fructus Crataegi (parched to brown)
Soak, the red bean powder of the 1.5% of the gross weight of addition food and water and the 0.05%NaHCO of the 1.5% of material gross weight3, it is allowed to
Easily boil, remove raw meat, except bitter taste, after 80 DEG C of 15min that precook, add and adjust pH value 5.5 with citric acid, add food and the gross weight of water
The 0.02% pectase 55 DEG C insulation enzymolysis 2h of amount, add the gross weight of food and water 1% hemicellulase, add food and
0.56% cellulase of the gross weight of water, adds 0.5% papain of the gross weight of food and water, addition food and water
0.04% phytase 1h of gross weight, mixes Fructus Hordei Germinatus, Massa Medicata Fermentata 15h, filters, sterilization, stand-by (solid content 8%～10%)；
(2) preparation Eurycoma longifolia extracting solution: by Eurycoma longifolia water blancing 2min, cooling, by material: water is that the weight ratio of 1:10 adds
Water is pulled an oar, and then mixes with moringa powder, Fructus Amomi Rotundus, Fructus Amomi, Rhizoma Polygonati Odorati, Semen Raphani, adds water to 2.5 times of the initial water yield, decocts,
Extraction supernatant, stand-by (solid content 4%～6%), Eurycoma longifolia, moringa powder, Fructus Amomi Rotundus, Fructus Amomi, Rhizoma Polygonati Odorati, the weight of Semen Raphani
Amount is respectively as follows: 2200g, 1100g, 150g, 120g, 150g, 110g；
(3) the preparation of Eurycoma longifolia nutritional solution: by food enzymolysis solution with Eurycoma longifolia extracting solution by weight the proportions for 4:1
It is mixed, mixed liquor is put in autoclave, be heated to 90 DEG C, and keep 5min, Eurycoma longifolia nutritional solution can be obtained；
(4) fermentation: adding culture fluid in Eurycoma longifolia nutritional solution, Eurycoma longifolia nutritional solution and culture fluid weight ratio are 10:1, add
Enter lactic acid bacteria, sodium chloride and acetic acid mixed fermentation 1day, add the breast of above-mentioned lactic acid bacteria, sodium chloride and acetic acid each half weight
Acid bacterium, sodium chloride and acetic fermentation, obtain fermentation liquid, lactic acid bacteria, sodium chloride, the weight of total consumption of acetic acid be respectively 120g,
Sodium chloride is 9g, and acetic acid is 6g；
The preparation method of culture fluid is: maltose is soluble in water, puts into compound vitamin, after stirring after standing 20min
Heated and boiled, adds Ovum Gallus domesticus album after being cooled to room temperature, stirs, stand-by, maltose, water, compound vitamin, the weight of Ovum Gallus domesticus album
Amount ratio is 100:10000:3:10.
(5) emulsifying: adding emulsifying agent in fermentation liquid, cross leaching clear liquid, after pasteurization, packaging gets product, emulsifying agent is food
With gelatin or carboxymethyl cellulose.
Finished product can cream processed, powder process, be processed further for preparation or the cosmetic material of oral liquid, this course of processing not as
This case inventive point, uses conventional prior art to complete, and in the present invention, each raw material all uses commercial goods.
Step (3) in, after mixed liquor is put into autoclave, add yeast extract 7.5%, dextrinosan 10%-20%, Fructus Citri Limoniae
Acid 0.15%-0.25%, sodium citrate 0.05%, L malic acid 0.05%, VC0.03%, it is equipped with food additive: xylitol,
0.07%-0.12% xanthan gum 0.5% arabic gum, 0.15% pectin, 0.15% sodium alginate, 0.12%-0.23% carboxymethyl cellulose
Sodium, 0.2%-0.8% fatty acid cane sugar ester emulsifying agent, 0.05% 3 polyglycerin ester, 0.2% Fructus Jujubae essence, beverage flavor agent 0.5% ethyl
Vanillin (bean milk powder essence), food coloring 0.02% phylloxanthin, 1% lecithin (source that Beijing is green, purity is 15%), 0.06%
Whole or a portion in daidzein, black vinegar, polydextrose, nicotinic acid, calcium pantothenate, → allocate, filter → sterilizing (will
Mixed liquor is put in autoclave, is heated to 90 DEG C, and keeps 5min) → directly beverage can be administered orally by Eurycoma longifolia, then need not
Step fermentation (4) and step emulsifying (5), above-mentioned percentage ratio is all that each material mixes with food enzymolysis solution and Eurycoma longifolia extracting solution
The percentage ratio of the gross weight after conjunction.
Control experiment
Select the healthy male mice 30 with area, body weight 20 ± 2g, be divided into 3 groups at random: matched group (being labeled as A),
Eurycoma longifolia fermentation liquid group group (being labeled as B) and Eurycoma longifolia group (being labeled as C),
The distilled water of A group employing 0.02ml/ (kg d), continuous gavage 10d,
The Eurycoma longifolia fermentation liquid that the embodiment of the present invention 1 of B group employing 2.6g/ (kg d) is given, continuous gavage 10d,
The Eurycoma longifolia distilled water solution of C group employing 2.6g/ (kg d), continuous gavage 10d,
Adaptability swimming secondary, each 10min is carried out during gavage.
Swimming with a load attached to the body is tested
After last gavage 30min, 3 treated animals all randomly select 5, and the mice of root of the tail portion load 5% body weight sheet lead is placed in trip
Swimming case went swimming.The depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, and record mice swimming time, i.e. mice start from swimming
Upstream face 10s is not had without the time come back to mouth and nose.
Fatigue be body under the conditions of certain environment, because of for a long time the heaviest, the most nervous work (including mental and physical)
A kind of physiological phenomenon of the temporary obvious reduction of work efficiency caused.Fatigue is the result of multifactor effect, the most directly
It is the decline of exercise tolerance with objectively performance, and swimming time is all the time by the important indicator as reflection exercise tolerance.
The mensuration of blood lactase acid
After last medication 30min, often organize remaining 5 animals and gather mouse tail blood immediately, measure blood lactase acid I；Then exist
Adopt tail blood after swimming trunk went swimming (not bearing a heavy burden) 20min immediately, measure blood lactase acid II；After rest 40min, again adopt tail blood,
Measure blood lactase acid III.Assay method is carried out according to the explanation of full blood lactic test kit.
The generation of blood lactase acid and the mensuration of degradation rate
The generating rate of blood lactase acid=(rest value before blood lactase acid peak-motion)/move to time × 100% used by peak；Blood breast
Acid degradation speed=(blood lactase acid value after blood lactase acid peak-rest 40min)/40min × 100%.
Statistical analysis
Data acquisition SPSS11.5 software processes, each experimental group uses single factor test methods analyst to compare two-by-two.
Experimental result
The impact on mice heavy burden swimming time of the Eurycoma longifolia fermentation liquid
Eurycoma longifolia fermentation liquid the results are shown in Table 1 to the impact of mice heavy burden swimming time.As shown in Table 1, B group mice bears a heavy burden
Swimming time dramatically increases (P ＜ 0.05) than A group and C group, shows that Eurycoma longifolia fermentation liquid has obvious anti-fatigue effect.
The impact on mice heavy burden swimming time of the table 1 Eurycoma longifolia fermentation liquid
Originally test result indicate that: mice swimming time is had no significant effect by A and the C group that this institute is selected, and east leather Ah
In fermentation liquid can extend the swimming time of mice significantly, thus prompting Eurycoma longifolia fermentation liquid can significantly improve the fortune of mice
Dynamic endurance.
Eurycoma longifolia fermentation liquid mice is swum before and after Serum lactic acid content and production of lactic acid and the impact of degradation rate
As shown in Table 2, after each group mouse stomach 10d, before mouse movement, between each group of blood lactase acid value, there is no notable difference.Mice
After carrying out the strenuous exercise of short time (20min), C group and A group do not have difference, B group blood lactase acid value to be all remarkably higher than matched group,
No significant difference between each administration group.
By table 2 it has been also found that after motion rest 40min, compared with A group, the blood lactase acid value (blood lactase acid of B, C group mice
III) all significantly reduce.Although B, C group blood lactase acid generating rate is significantly higher than A group, but the equal C of blood lactase acid degradation rate of B group
Group significantly increases, and the ability of prompting Eurycoma longifolia fermentation liquid aerobic metabolism is strengthened.
Table 2 Eurycoma longifolia fermentation liquid is on the impact of Serum lactic acid content before and after mice swimming
The accumulation of metabolite is that body produces tired main cause, and wherein, the increase with H+ concentration of piling up of lactic acid is important
Factor.Violent physical exertion makes lactic acid pile up in vivo, and blood pH reduces, it is suppressed that phosphofructose swashs the multiple kinds of energy generations such as acid
The activity of enzyme during thanking, makes glycolysis be affected, and ATP supplies minimizing, causes muscle fatigue.After mice swimming 20min,
C group and more blank group of B treated animal Serum lactic acid content substantially increase, and production of lactic acid speed increases the most accordingly, in conjunction with B treated animal
The interpretation of result that swimming time is obviously prolonged, with Eurycoma longifolia and enzymatic hydrolysis and fermentation liquid thereof, this promotes that body anaerobic metabolism level has
Close.
After additionally mice swimming stops, 20～50min is convalescent period of blood lactase acid after motion.Convalescent period after exercise, body
Needing a large amount of oxygen of inspiration, now body is in addition to mainly with aerobic metabolism mode energy supply, the oxygen debt in strenuous exercise to be repaid,
The removing of lactic acid completes by aerobic metabolism now just.
Owing to Eurycoma longifolia fermentation liquid is in addition to containing sugar, electrolyte, include by force possibly together with little molecule small peptide, polymerization sugar, aminoacid
The taurine changed and abundant plant flavone, vitamin B group, VC and various fatsoluble vitamin, choline, the zinc etc. of high concentration
Functional component has great relation.Little molecule small peptide directly can be absorbed by intestinal, energy energy supply rapidly, has accelerated motion muscle tired
The effect that labor is recovered.
Although disclosing embodiments of the invention for the purpose of illustration, but it will be appreciated by those skilled in the art that: without departing from this
In invention and spirit and scope of the appended claims, various replacements, to change and modifications be all possible, therefore, and the present invention's
Scope is not limited to embodiment disclosure of that.
Claims (6)
1. the preparation method of an Eurycoma longifolia fermentation liquid, it is characterised in that: comprise the steps:
Preparation food enzymolysis solution: by etc. the purple rice of weight, Semen setariae, Semen Coicis, Poria, Fructus Jujubae, Fructus Mume, Pericarpium Citri Reticulatae, Fructus Crataegi (parched to brown)
Soak, the red bean powder of the 1.5% of the gross weight of addition food and water and the 0.05%NaHCO of the 1.5% of material gross weight3, 80 DEG C
Precook after 15min, add and adjust pH value 5.5 with citric acid, add the 0.02% pectase 55 DEG C insulation enzyme of the gross weight of food and water
Solve 2h, add 1% hemicellulase of the gross weight of food and water, add 0.56% cellulase of the gross weight of food and water,
Add 0.5% papain of the gross weight of food and water, add 0.04% phytase 1h of the gross weight of food and water, mix wheat
Bud, Massa Medicata Fermentata 15h, filter, and sterilization obtains food enzymolysis solution, stand-by；
(2) preparation Eurycoma longifolia extracting solution: by Eurycoma longifolia water blancing 2min, cooling, by material: water is that the weight ratio of 1:10 adds
Water is pulled an oar, and then mixes with moringa powder, Fructus Amomi Rotundus, Fructus Amomi, Rhizoma Polygonati Odorati, Semen Raphani, adds water to 2.5 times, decocts, and extracts supernatant
Liquid, obtains Eurycoma longifolia extracting solution, stand-by；
(3) the preparation of Eurycoma longifolia nutritional solution: by food enzymolysis solution with Eurycoma longifolia extracting solution by weight the proportions for 4:1
It is mixed, mixed liquor is put in autoclave, be heated to 90 DEG C, and keep 5min, Eurycoma longifolia nutritional solution can be obtained；
(4) fermentation: adding culture fluid in Eurycoma longifolia nutritional solution, Eurycoma longifolia nutritional solution and culture fluid weight ratio are 10:1, add
Enter lactic acid bacteria, sodium chloride and acetic acid mixed fermentation 1day, add the breast of above-mentioned lactic acid bacteria, sodium chloride and acetic acid each half weight
Acid bacterium, sodium chloride and acetic fermentation, obtain fermentation liquid；
(5) emulsifying: adding emulsifying agent in fermentation liquid, cross leaching clear liquid, after pasteurization, packaging gets product, emulsifying agent is food
With gelatin or carboxymethyl cellulose.
The preparation method of Eurycoma longifolia fermentation liquid the most according to claim 1, it is characterised in that: the food that step is (1) described
The content of the solid content of enzymolysis solution is 8%～10%.
The preparation method of Eurycoma longifolia fermentation liquid the most according to claim 1, it is characterised in that: step (2) described in east
The solid content of leather Ali's extracting solution is 4%～6%.
The preparation method of Eurycoma longifolia fermentation liquid the most according to claim 3, it is characterised in that: step (2) described in east
Leather Ali, moringa powder, Fructus Amomi Rotundus, Fructus Amomi, Rhizoma Polygonati Odorati, the parts by weight of Semen Raphani be respectively as follows: 20～25 parts, 5～10 parts, 1～2
Part, 1～2 part, 1～2 part, 1～2 part.
The preparation method of Eurycoma longifolia fermentation liquid the most according to claim 1, it is characterised in that: the lactic acid that step is (3) described
Bacterium, sodium chloride, the parts by weight of total consumption of acetic acid are respectively 100～120 parts, 8～10 parts, and acetic acid is 4～6 parts.
The preparation method of Eurycoma longifolia fermentation liquid the most according to claim 5, it is characterised in that: the cultivation that step is (3) described
The preparation method of liquid is: maltose is soluble in water, puts into compound vitamin after standing 20min, and the post-heating that stirs boils,
Adding Ovum Gallus domesticus album after being cooled to room temperature, stir, stand-by, maltose, water, compound vitamin, the weight ratio of Ovum Gallus domesticus album are
100:10000:3:10。
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| CN103385925A (en) * | 2013-07-18 | 2013-11-13 | 中国人民武装警察部队后勤学院 | Fructus cannabis enzymolysis fermentation liquor having fatigue resistance and its preparation method and use |
| CN104664270A (en) * | 2014-07-09 | 2015-06-03 | 普正药业股份有限公司 | Fresh and alive traditional Chinese medicine fruit-vegetable enzyme and preparation method thereof |
| CN105167096A (en) * | 2015-11-02 | 2015-12-23 | 广西金臣科技有限公司 | Okra enzyme nutrient solution and preparation method thereof |
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| CN111616353A (en) * | 2019-12-31 | 2020-09-04 | 北京中医药大学 | Prunus humilis composite fermentation broth for regulating intestinal flora and preparation method thereof |
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