CN105954450B - A kind of pouzolzia zeylanica medicinal material or the HPLC finger-prints and its method for building up of pouzolzia zeylanica general flavone and application - Google Patents
A kind of pouzolzia zeylanica medicinal material or the HPLC finger-prints and its method for building up of pouzolzia zeylanica general flavone and application Download PDFInfo
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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- G01N30/8686—Fingerprinting, e.g. without prior knowledge of the sample components
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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Abstract
The present invention relates to Chinese medicine detection technique field, specifically discloses a kind of pouzolzia zeylanica medicinal material or the HPLC finger-prints and its method for building up of pouzolzia zeylanica general flavone and application.The method for building up of the finger-print comprises the following steps：S1. precision weighs pouzolzia zeylanica medicinal material, and test solution is prepared；S2. test solution is analyzed using high performance liquid chromatograph, obtains finger-print.The finger-print can more fully response sample characteristic peak information, and this method stability and reappearance are preferable；Using the finger-print true and false or quartile length can be carried out to pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone.
Description
Technical field
The present invention relates to Chinese medicine detection technique field, and in particular to a kind of pouzolzia zeylanica medicinal material or pouzolzia zeylanica total
The HPLC finger-prints and its method for building up of flavones and application.
Background technology
Pouzolzia zeylanica (scientific name：Pouzolzia zeylanica var.microphylla) it is Urticaceae Pouzolzia
Under a mutation.Tropical Asian area blazons, and originates in the Chinese yunnan southeast, Guangxi, Guangdong, Fujian, South Jiangxi, platform
Gulf；There is detoxicating, relieving inflammation；It is scorching swollen for treating furuncle sore.Flavones ingredient is one of active ingredient of pouzolzia zeylanica.Mesh
Before, lack a kind of accurate detection pouzolzia zeylanica medicinal material true and false and quality, and the method for pouzolzia zeylanica general flavone quality.
After traditional Chinese medicine fingerprint refers to that certain (or certain place of production) Chinese medicine or Chinese patent drug are appropriately processed, using certain point
Analysis means, what is obtained can indicate the collection of illustrative plates at the shared peak of the Chinese medicine or Chinese patent drug medicinal property.Finger-print is whole from the traditional Chinese medical science
From the viewpoint of body idea and herbal medicine efficacy come from a variety of chemical substance comprehensive functions, it can establish more objective, overall and more
The overall evaluation system of index, and by the global feature of collection of illustrative plates, can be than more fully reflecting chemical composition contained by Chinese medicine.
The content of the invention
The technical problems to be solved by the invention are, in order to overcome above-mentioned deficiency existing in the prior art, there is provided a kind of
The HPLC finger-prints of pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone.
Above-mentioned technical problem to be solved by this invention, is achieved by the following technical programs：
A kind of method for building up of the HPLC finger-prints of pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone, comprising as follows
Step：
S1. precision weighs pouzolzia zeylanica medicinal material, and test solution is prepared；
S2. test solution is analyzed using high performance liquid chromatograph, obtains finger-print.
Preferably, the test solution is prepared by the method comprised the following steps：
S11. pouzolzia zeylanica medicinal material is weighed, 5~15 times is added and measures (ml/g) petroleum ether refluxing extraction 1~5 time, every time
0.5~3h, filtration, discards petroleum ether extract, obtains filter residue；
S12. 5~15 times of amount (ml/g) alcohol refluxs of filter residue are extracted 1~5 time, 0.5~3h, filtration, merging every time is filtered
Liquid, is concentrated into no alcohol taste, is dissolved in water, centrifuges, and takes supernatant to add water to adjust concentration to 0.1~0.5g crude drugs/ml and adjusts pH
To 4.5~5.5, sample solution is obtained；
S13. the sample solution that aspiration step S12 is prepared, general flavone, loading flow velocity are enriched with AB-8 macroporous resin columns
0.5~3BV/h, after loading, is cleaned with the water elution of 1~5BV, with the ethanol that 2~5BV volume fractions are 50~70%
Eluted with the flow velocity of 1~3BV/h, collect ethanol eluate, filtered with filter membrane, obtain test solution.
Preferably, the method for building up of the HPLC finger-prints, it is A~M chosen from the followings in any one or
It is multinomial：
A. in step S11., 8~12 times of amounts (such as 8,9,10,11 or 12 times of amounts) petroleum ether is added；
B. in step S11., refluxing extraction 2~4 times (such as 2,3 or 4 times)；
C. in step S11., the time of each refluxing extraction is 0.5~2h (such as 0.5,1,1.5 or 2h)；
D. in step S12., 8~12 times of amounts (such as 8,9,10,11 or 12 times of amounts) ethanol is added；
E. in step S12., the volume fraction of ethanol for 50~95% (such as 50%, 60%, 70%, 80%, 90% or
95%)；
F. in step S12., refluxing extraction 2~4 times (such as 2,3 or 4 times)；
G. in step S12., the time of each refluxing extraction is 0.5~2h (such as 0.5,1,1.5 or 2h)；
H. in step S12., time of centrifugation is 5~50min, 1500~5000rpm of rotating speed (such as the time for 5~
20min, rotating speed are 2000~4000rpm；Or the time is 10min, rotating speed 3000rpm)；
I. in step S12., supernatant is taken to add water to adjust concentration to 0.2~0.4g crude drugs/ml (such as 0.2,0.3 or 0.4g
Crude drug/ml)；
J. in step S12., pH to 4.5,4.8,5.0,5.2 or 5.5 are adjusted.
K. in step S13., loading flow velocity is 1~3BV/h (such as 1BV/h, 2BV/h or 3BV/h)；
L. in step S13., cleaned with the water elution of 2~4BV (such as 2BV, 3BV or 4BV)；
M. in step S13., with the second that 2~4BV (such as 2BV, 3BV or 4BV) volume fraction is 50%, 60% or 70%
Alcohol is eluted with the flow velocity of 1BV/h or 1BV/h.
Above-mentioned times amount represents to add the milliliter number of petroleum ether or ethanol in every gram of pouzolzia zeylanica medicinal material.
Above-mentioned unit 1BV is volume unit, represents equivalent to 1 times macroreticular resin column volume of volume.
Preferably, in step S13. before general flavone is enriched with AB-8 macroporous resin columns, accurate aspiration step S12 is prepared into
The sample solution arrived；Before being filtered with filter membrane, ethanol eluate will be collected, with volume fraction for 50~70% (such as 50%,
60% or ethanol constant volume 70%).
Preferably, the chromatographic condition of high performance liquid chromatograph analysis is in step S2：Using ACQUITY UPLC BEH
SHIELD RP18 chromatographic columns；Gradient elution is carried out by mobile phase of the formic acid water of acetonitrile -0.2%；Flow velocity is 0.2~0.5mL/
Min, Detection wavelength are 30~330nm；Sample size is 0.2~10 μ l；
Phase gradient elution requirement is：0~22min, the volume fraction of acetonitrile, which becomes, turns to 15%~19%；22~26min, second
The volume fraction of nitrile, which becomes, turns to 19%~24%；26~32min, the volume fraction of acetonitrile, which becomes, turns to 24%~24%；32~
40min, the volume fraction of acetonitrile, which becomes, turns to 24%~38%.
It is highly preferred that the chromatographic condition that high performance liquid chromatograph is analyzed in step S2 is：Use specification for 21mm ×
150mm, 1.7 μm of ACQUITY UPLC BEH SHIELD RP18 chromatographic columns；Using the formic acid water of acetonitrile -0.2% as mobile phase into
Row gradient elution；Flow velocity is 0.3mL/min, Detection wavelength 330nm；Sample size is 0.2 μ l；
Phase gradient elution requirement is：0~22min, the volume fraction of acetonitrile, which becomes, turns to 15%~19%；22~26min, second
The volume fraction of nitrile, which becomes, turns to 19%~24%；26~32min, the volume fraction of acetonitrile, which becomes, turns to 24%~24%；32~
40min, the volume fraction of acetonitrile, which becomes, turns to 24%~38%.
Above-mentioned 0.2% formic acid water is represented in water containing the formic acid that volume fraction is 0.2%.
It is to pass through the present invention also provides a kind of pouzolzia zeylanica medicinal material or the HPLC finger-prints of pouzolzia zeylanica general flavone
Method for building up described in any of the above-described is established to obtain.
The standard HPLC finger-prints of also a kind of pouzolzia zeylanica medicinal material of the present invention or pouzolzia zeylanica general flavone, are selected from
(1) any one of~(3)：
(1) single batch or multiple batches of pouzolzia zeylanica genunie medicinal materials and the foundation side described according to any of the above-described are used
The pouzolzia zeylanica Herbal HPLC Fingerprint that method obtains；
(2) will obtain using multiple batches of pouzolzia zeylanica genunie medicinal materials and according to the method for building up described in any of the above-described
HPLC finger-prints control spectrogram is made by average method or median method；
(3) it has 11 characteristic peaks, and retention time is respectively 6.814 ± 0.05min, 11.737 ± 0.05min, 12.307
± 0.05min, 12.987 ± 0.05min, 18.821 ± 0.05min, 19.888 ± 0.05min, 21.115 ± 0.05min,
24.684 ± 0.05min, 26.924 ± 0.05min, 27.643 ± 0.05min and 30.297 ± 0.05min.
According to method provided by the invention, pouzolzia zeylanica medicinal material is determined, it is determined that above-mentioned 11 characteristic peaks.
The present invention also provides a kind of method for detecting pouzolzia zeylanica quality of medicinal material or pouzolzia zeylanica general flavone quality, bag
Include following steps：The method for building up of HPLC finger-prints first as described in any of the above-described, establishes pouzolzia zeylanica to be detected
The HPLC finger-prints of medicinal material or pouzolzia zeylanica general flavone；Then it is yellow with above-mentioned pouzolzia zeylanica medicinal material or pouzolzia zeylanica total
The standard HPLC finger-prints of ketone are compared, and are finally judged the quality of pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone and are
No qualification.
The judgement can judge according to the similarity of finger-print, if the similarity of the two finger-print is not less than
0.85, then to be up-to-standard；If less than 0.85, to be unqualified；The fingerprint that the similarity is promulgated by national Bureau of Drugs Supervision
Collection of illustrative plates similarity evaluation software obtains.
The present invention also provides a kind of above-mentioned pouzolzia zeylanica medicinal material or the HPLC finger-prints of pouzolzia zeylanica general flavone, or
The standard HPLC finger-prints of above-mentioned pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone differentiate pouzolzia zeylanica medicinal material or
Application in the true and false or quality of pouzolzia zeylanica general flavone.
Beneficial effect：(1) present invention establishes pouzolzia zeylanica medicinal material first or the HPLC of pouzolzia zeylanica general flavone refers to
The standard HPLC finger-prints of line collection of illustrative plates and pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone；(2) finger-print with
And standard finger-print can more fully response sample characteristic peak information, and this method stability and reappearance are preferable；(3)
Using the finger-print and standard finger-print the true and false can be carried out to pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone
Or quartile length.
Brief description of the drawings
Fig. 1 is the HPLC standard finger-prints of pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone.
Fig. 2 is the precision test of the HPLC standard finger-prints of pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone
Figure.
Fig. 3 is the repeated experiment of the HPLC standard finger-prints of pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone
Figure.
Fig. 4 is the stability experiment of the HPLC standard finger-prints of pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone.
Fig. 5 is the HPLC standard finger-prints and liquid-matter (spectrum) connection of pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone
Compare figure with total ion current figure；Wherein A is total ion current figure, and B is fingerprint image spectrogram.
Embodiment
The present invention is explained further below in conjunction with specific embodiment, but embodiment does not do any type of limit to the present invention
It is fixed.
The person that is not specified actual conditions in embodiment, the condition suggested according to normal condition or manufacturer carry out.Wherein, it is used
Production firm person is not specified in reagent or instrument, and being can be with conventional products that are commercially available.
The foundation of the HPLC finger-prints of 1 pouzolzia zeylanica medicinal material of embodiment or pouzolzia zeylanica general flavone
1st, instrument and reagent
Waters SYNAPT G2-S Q-TOF mass spectrographs (Waters, US)
ACQUITY UPLC types Ultra Performance Liquid Chromatography instrument (waters companies of the U.S.)
ACQUITY UPLC BEH SHIELD RP18 chromatographic columns (21mm × 150mm, 1.7 μm)
AY120 type pallets electronic analytical balance (Japanese Shimadzu Corporation)
DK-S24 types electric-heated thermostatic water bath (the upper grand experimental facilities Co., Ltd of Nereid)
LABOROTA4000 Rotary Evaporators (German Heidolph).
Acetonitrile is chromatographically pure (Ou Pusen boards), and liquid phase is Watson distilled water with water, and ethanol is industrial alcohol, other reagents
It is pure to analyze.
Pouzolzia zeylanica medicinal material is purchased from Guangxi Yulin, is Urticaceae Sri Lanka Pouzolzia Herb through Guangdong Pharmaceutical University professor Liu Jizhu identification
Belong to the herb of pouzolzia zeylanica Pouzolzia zeylanica (L.) Benn.var.microphylla (Wedd.) W.T.Wang.
2nd, experimental method
The preparation of 2.1 test solutions
Take pouzolzia zeylanica medicinal material to be crushed, weigh medicinal material coarse powder 25g, be placed in round-bottomed flask, be separately added into 8 times of amounts
80 DEG C of petroleum ether refluxing extraction 2 times, each 1h.After filter residue flings to petroleum ether, add 8 times and measure 70% alcohol refluxs extraction 3 times, often
Secondary 1h, merges 3 filtrates, is concentrated under reduced pressure into no alcohol taste, adds water to make fully to dissolve, and centrifuges (3000r/min) 10min, takes supernatant
Liquid adds water to adjust concentration to 0.3g crude drugs/ml and adjusts pH to 5.Precision draws the above-mentioned sample solutions of 75ml and is added in AB-8 macropore trees
Fat column (1.5 × 8cm of Φ), loading flow velocity 2BV/h, is added water 2BV to clean, adds 60% ethanol 2BV to be eluted with 2BV/h, collects elution
Liquid, adds 60% ethanol to be settled in 100ml volumetric flasks, shakes up, 0.22 μm of filter membrane filtration, to obtain the final product.The ethanol percentage composition
It is volumn concentration.
2.2 chromatographic condition
Specification is used as 21mm × 150mm, 1.7 μm of ACQUITY UPLC BEH SHIELD RP18 chromatographic columns；With second
(volume) % of nitrile -0.2 formic acid waters carry out gradient elution for mobile phase；Flow velocity is 0.3mL/min, Detection wavelength 330nm；Sample introduction
Measure as 0.2 μ l；
Phase gradient elution requirement is：0~22min, the volume fraction of acetonitrile, which becomes, turns to 15%~19%；22~26min, second
The volume fraction of nitrile, which becomes, turns to 19%~24%；26~32min, the volume fraction of acetonitrile, which becomes, turns to 24%~24%；32~
40min, the volume fraction of acetonitrile, which becomes, turns to 24%~38%.
By test solution, detected according to the chromatographic condition described in 2.2, obtained pouzolzia zeylanica medicinal material or racemosus fog
The HPLC standard finger-prints of Pueraria lobota general flavone are as shown in Figure 1.Fig. 1 shows that the finger-print has 11 characteristic peaks, retention time point
Not Wei 6.814min, 11.737min, 12.307min, 12.987min, 18.821min, 19.888min, 21.115min,
24.684min, 26.924min, 27.643min and 30.297min.
3rd, methodological study
3.1 Precision Experiment
Precision weighing medicinal material coarse powder 25g, test solution is prepared by the method under 2.1, is carried out by 2.2 chromatographic condition
Measure, continuous sample introduction 6 times, the RSD of the relative peak area of each chromatographic peak are less than 2.0%, and relative retention time RSD is less than 2.0%,
The similarity of finger-print is calculated by 2.4 lower methods, is respectively 0.993,0.996,0.992,0.997,0.995,0.996
(see Fig. 2), shows that instrument precision is good.
3.2 repeated experiment
Precision weighing medicinal material coarse powder 25g, prepares 6 parts of test solution, by 2.2 chromatography by the method under 2.1 is parallel
Condition is measured, the results showed that, the RSD of the relative retention time of each chromatographic peak is respectively less than 7%, and the RSD of relative peak area is equal
Less than 5%, the similarity of finger-print is calculated by 2.4 lower methods, is respectively 0.997,0.998,0.998,0.995,0.997
(see Fig. 3), illustrates that the repeatability of this method is good.
3.3 stability experiment
Precision weighing medicinal material coarse powder 25g, prepares test solution by the method under 2.1 is parallel, places at room temperature, respectively
It is measured at 0,2,4,12,24 by 2.2 chromatographic condition, the RSD of the relative retention time of each chromatographic peak is less than 3%；Phase
5% is less than to the RSD of peak area, the similarity of finger-print is calculated by 2.4 lower methods, be respectively 0.993,0.987,
0.997th, 0.994,0.997,0.996 (see Fig. 4), shows test solution interior stabilization when 24 is small.
The identification at the HPLC Fingerprints peak of 2 pouzolzia zeylanica medicinal material of embodiment or pouzolzia zeylanica general flavone
Test solution is prepared with reference to 2.1 in embodiment 1.
Chromatographic condition is with reference to 2.2 in embodiment 1.
It is prepared by reference substance solution：Precision weighs 6,7- dimethyl -1,4- dihydro -2,3- Quinoxalinediones, apiolin -6-C-
A-L- arabinoses 8-C- β-D-Glucose glycosides, apiolin -6-C- [α-L-arabinose (1 → 6)-β-D-Glucose], kaempferia galamga
Phenol -3-O- [α-L- rhamnoses (1 → 6)-β-D-Glucose] -7-O- α-L- glucosides, Quercetin -3-O- [α-L- rhamnoses
(1 → 6)-β-D-Glucose] -7-O- alpha-L-rhamnosides, Quercetin -3,7-2-O- α-L- rhamnopyranosyloxyhies glucosides, Kaempferol -3-
O- [α-L- rhamnoses (1 → 6)-β-D-Glucose] -7-O- alpha-L-rhamnosides and Kaempferol -3,7-2-O- α-L- rhamnopyranosyloxyhies
Glucosides reference substance is appropriate, is configured to reference substance solution with proper amount of methanol dissolving, to obtain the final product.
Mass Spectrometer Method condition：Electric spray ion source (ESI), negative ion mode；Capillary voltage (capillary
voltage)3000V；Collision voltage (collision, CE) is 30~40V；Taper hole voltage (sampling cone) 40V；Go molten
Agent temperature (TEM) is 350 DEG C；Nitrogen ionizes for assistant spray and removes solvent gas, and it is 800L/h to remove solvent gas product flow；Cone
Gas flow hole cone gas flow) it is 50L/h.
By UPLC-ESI-Q-TOF-MS detect to obtain in pouzolzia zeylanica medicinal material the total ion current figure of each chemical composition with
Fingerprint image spectrogram (see Fig. 5), and combine each chemical composition extraction ion flow graph and with reference substance chromatogram, pertinent literature data
11 characteristic peaks in finger-print are carried out further chemical composition confirmation, the results are shown in Table 1 by contrast.
The discriminating and identification of 1 chromatographic peak of table
Table 1 the result shows that, contained in finger-print and standard finger-print of the invention in pouzolzia zeylanica known
Multiple compounds, have height reliability.
The method that embodiment 3 detects the pouzolzia zeylanica medicinal material true and false using standard finger-print
Random commercially a collection of pouzolzia zeylanica medicinal material detects its true and false.
The method for building up of HPLC finger-prints first as described in embodiment 1, establishes pouzolzia zeylanica medicinal material to be detected
HPLC finger-prints；Then carried out with the pouzolzia zeylanica medicinal material HPLC standard finger-prints established by 1 method of embodiment
Compare；The fingerprint similarity evaluation software promulgated by national Bureau of Drugs Supervision measures the HPLC finger-prints and mark of this batch of medicinal material
The similarity of quasi- HPLC finger-prints is 0.9, it is possible to determine that, this batch of medicinal material is qualified pouzolzia zeylanica medicinal material.
Claims (7)
1. a kind of method for building up of the HPLC finger-prints of pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone,
It is characterized in that, comprise the following steps：
S1. precision weighs pouzolzia zeylanica medicinal material, and test solution is prepared；
S2. test solution is analyzed using high performance liquid chromatograph, obtains finger-print；
The chromatographic condition of high performance liquid chromatograph analysis is in step S2：Using ACQUITY UPLC BEH SHIELD RP18 colors
Compose column；Gradient elution is carried out by mobile phase of the formic acid water of acetonitrile -0.2%；Flow velocity is 0.2 ~ 0.5mL/min, Detection wavelength for 30 ~
330nm；Sample size is 0.2 ~ 10 μ l；
Condition of gradient elution is：0~22min, the volume fraction of acetonitrile, which becomes, turns to 15% ~ 19%；22~26min, the volume of acetonitrile
Fraction, which becomes, turns to 19% ~ 24%；26~32min, the volume fraction of acetonitrile, which becomes, turns to 24% ~ 24%；32~40min, the volume of acetonitrile
Fraction, which becomes, turns to 24% ~ 38%；
The test solution is prepared by the method comprised the following steps：
S11. pouzolzia zeylanica medicinal material is weighed, 5 ~ 15 times is added and measures petroleum ether refluxing extraction 1 ~ 5 time, every time 0.5 ~ 3h, filtration,
Petroleum ether extract is discarded, obtains filter residue；
S12. 5 ~ 15 times of amount alcohol refluxs of filter residue are extracted 1 ~ 5 time, 0.5 ~ 3h, filters, merging filtrate, is concentrated into nothing every time
Alcohol taste, is dissolved in water, centrifuges, and takes supernatant to add water to adjust concentration to 0.1 ~ 0.5g crude drugs/ml and adjusts pH to 4.5 ~ 5.5, obtains
Sample solution；
S13. the sample solution that aspiration step S12 is prepared, general flavone, loading flow velocity 0.5 are enriched with AB-8 macroporous resin columns
~ 3BV/h, after loading, is cleaned with the water elution of 1 ~ 5 BV, then with the ethanol that 2 ~ 5BV volume fractions are 50 ~ 70% with 1 ~ 3
The flow velocity elution of BV/h, collects ethanol eluate, is filtered with filter membrane, obtain test solution.
2. the method for building up of HPLC finger-prints according to claim 1, it is characterised in that in A ~ M chosen from the followings
Any one or multinomial：
A. in step S11., 8 ~ 12 times of amount petroleum ethers are added；
B. in step S11., refluxing extraction 2 ~ 4 times；
C. in step S11., the time of each refluxing extraction is 0.5 ~ 2h；
D. in step S12., 8 ~ 12 times of amount ethanol are added；
E. in step S12., the volume fraction of ethanol is 50 ~ 95%；
F. in step S12., refluxing extraction 2 ~ 4 times；
G. in step S12., the time of each refluxing extraction is 0.5 ~ 2h；
H. in step S12., the time of centrifugation is 5 ~ 50min, 1500 ~ 5000rpm of rotating speed；
I. in step S12., supernatant is taken to add water to adjust concentration to 0.2 ~ 0.4g crude drugs/ml；
J. in step S12., pH to 4.5,4.8,5.0,5.2 or 5.5 are adjusted；
K. in step S13., loading flow velocity is 1 ~ 3BV/h；
L. in step S13., cleaned with the water elution of 2 ~ 4 BV；
M. in step S13., eluted with the ethanol that 2 ~ 4BV volume fractions are 50%, 60% or 70% with the flow velocity of 1BV/h.
3. the method for building up of HPLC finger-prints according to claim 1, it is characterised in that with AB-8 in step S13.
Before macroporous resin column enrichment general flavone, sample solution that accurate aspiration step S12 is prepared；Before being filtered with filter membrane, it will receive
The ethanol eluate volume fraction of collection is 50 ~ 70% ethanol constant volume.
4. the method for building up of HPLC finger-prints according to claim 1, it is characterised in that high-efficient liquid phase color in step S2
Spectrometer analysis chromatographic condition be：Specification is used as 2.1mm × 150mm, 1.7 μm of ACQUITY UPLC BEH SHIELD
RP18 chromatographic columns；Flow velocity is 0.3 mL/min, Detection wavelength 330nm；Sample size is 0.2 μ l.
5. the HPLC finger-prints of a kind of pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone, it is characterised in that pass through right
It is required that 1 ~ 4 any one of them method for building up is established to obtain.
6. the standard HPLC finger-prints of a kind of pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone, it is characterised in that be selected from
（1）~（2）Any one of：
（1）Using single batch or multiple batches of pouzolzia zeylanica genunie medicinal materials and according to described in any one of claims 1 to 4
The pouzolzia zeylanica Herbal HPLC Fingerprint that method for building up obtains；
（2）Foundation side by the multiple batches of pouzolzia zeylanica genunie medicinal materials of use and described according to any one of claims 1 to 4
Control spectrogram is made by average method or median method in the HPLC finger-prints that method obtains.
7. the HPLC finger-prints of the pouzolzia zeylanica medicinal material or pouzolzia zeylanica general flavone described in claim 6 are differentiating racemosus
Application in the true and false or quality of Sri Lanka Pouzolzia Herb medicinal material or pouzolzia zeylanica general flavone.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610171641.2A CN105954450B (en) | 2016-03-23 | 2016-03-23 | A kind of pouzolzia zeylanica medicinal material or the HPLC finger-prints and its method for building up of pouzolzia zeylanica general flavone and application |
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| CN201610171641.2A CN105954450B (en) | 2016-03-23 | 2016-03-23 | A kind of pouzolzia zeylanica medicinal material or the HPLC finger-prints and its method for building up of pouzolzia zeylanica general flavone and application |
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| Publication Number | Publication Date |
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| CN105954450A CN105954450A (en) | 2016-09-21 |
| CN105954450B true CN105954450B (en) | 2018-04-27 |
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| US20120183631A1 (en) * | 2011-01-14 | 2012-07-19 | A-Shiou Hung-Chen | Ingredient extracted from pouzolzia zeylanica (l.) benn and its anti-acne formula |
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