CN105954397A - Method for simultaneously measuring contents of multiple skin disinfectants in wet tissue - Google Patents
Method for simultaneously measuring contents of multiple skin disinfectants in wet tissue Download PDFInfo
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- CN105954397A CN105954397A CN201610259740.6A CN201610259740A CN105954397A CN 105954397 A CN105954397 A CN 105954397A CN 201610259740 A CN201610259740 A CN 201610259740A CN 105954397 A CN105954397 A CN 105954397A
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- wet tissue
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/12—Preparation by evaporation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/12—Preparation by evaporation
- G01N2030/126—Preparation by evaporation evaporating sample
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- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention provides a method for simultaneously measuring the contents of multiple skin disinfectants in the wet tissue, and aims at providing the method which has simple operating method for simultaneously measuring the contents of multiple skin disinfectants in the wet tissue. The method uses a high performance liquid chromatography method to simultaneously measure the contents of chlorhexidine gluconate, chlorhexidine acetate, 2,4,4'-trichlorine-2'-phenoxyphenol, benzalkonium bromide, benzalkonium chloride and glutaraldehyde in the wet tissue. The invention belongs to the technical field of chemical detection.
Description
Technical field
The present invention provides a kind of assay method measuring disinfectant, specifically, is to examine simultaneously
The method of various skin disinfectant content in hygrometric towel；Belong to technical field of chemical detection.
Background technology
Wet paper towel is as the most emerging hygienic article, and purposes widely, can be used for individual
People's consumption and commercial multiple occasion, such as civil aviaton, family, hotel, dining room, travel outdoors
Deng, progressively favored by people with its convenience.Along with people's hygienic habit form and
The raising of quality of life, wet paper towel can be increasingly becoming the necessary of people's daily life.L Water Paper
Towel be exactly the one be made up of pure water, spun-laced nonwoven fabric and propylene glycol for wipe face wipe hands or
A kind of napkin of skin.
Wet tissue on market substantially can be divided into two classes: a class is that itself is sterilized, but can not
Sterilizing other article, the composition of skin protection is contained in the inside, can only do the skin moistening maintenance of skin.Separately
One class is that not only itself is sterilized, and other article may also function as the sterilization of disinfective action
Wet tissue, can be used as sterilization or the sterilization of skin abrasion, scuffing etc., the most on the packaging can
Indicate sterilization or the composition of sterilization, but common disinfectant all have certain injury to skin,
If using excess, then skin is brought serious injury.
Summary of the invention
For above-mentioned deficiency, it is an object of the invention to provide a kind of operational approach easy, can be same
Time quick wet tissue in the method for various skin disinfectant.
For solving above-mentioned technical problem, the technical scheme that the present invention provides is as follows:
In a kind of wet tissue of detection simultaneously, the method for various skin disinfectant content, is to use efficiently
Liquid chromatography measure simultaneously chlorhexidine gluconate in wet tissue, chlorhexidine acetate, 2,4,
4 "-three chloro-2 "-dihydroxy diphenyl ether, benzalkonium bromide, benzalkonium chloride and the content of glutaraldehyde.
Further, various skin disinfectant content in above-mentioned a kind of wet tissue of detection simultaneously
Method, it is characterised in that in described high effective liquid chromatography for measuring wet tissue, various skin disappears
Toxic agent content, includes following step successively:
1) squeeze out liquid component in wet tissue, collect liquid；
2) step 1 is measured) obtain liquid 1ml, it is transferred in 50ml volumetric flask, adds
30% ethanol, supersound extraction 5min, extract 2 times, united extraction liquid is also evaporated, and adds methanol
It is settled to 50ml, filters, inject high performance liquid chromatograph detection, measure chromatogram；
3) weigh chlorhexidine gluconate, chlorhexidine acetate respectively, 2,4,4 "-three is chloro-
2 "-dihydroxy diphenyl ether, benzalkonium bromide, benzalkonium chloride and glutaraldehyde, adds methanol and makes into concentration and be
0.02mg/mL, detects material list mark chromatograph volume mixed chromatogram.
Further, various skin disinfectant content in above-mentioned a kind of wet tissue of detection simultaneously
Method, described chromatographic condition is as follows:
Chromatographic column: Thermo AccliaimTM Mixed-Mode WCX-1 post；
Flowing phase: A: water；B:1mol/L Acetic acid-sodium acetate buffer, pH 6.0；C:
Methanol；
Gradient elution program: 0～1min, 3%～97%A, 2%B, 95%～1%C；1～
4min, 13%～87%A, 2%B, 85%～11%C；4～15.5min, 40%～31%A,
10%～19%B, 50%C；5min is balanced under 80%A, 15%B, 5%C；Flow velocity:
0.8mL/min；Column temperature: 35 DEG C；Sample size: 5 μ l；Detection wavelength: 258nm.
Further, various skin disinfectant content in above-mentioned a kind of wet tissue of detection simultaneously
Method, described high performance liquid chromatograph is Agilant12000 high performance liquid chromatograph.
Compared with prior art, the technical scheme that the present invention provides establishes high performance liquid chromatography
Measure various skin disinfectant content in wet paper towel simultaneously, complete eachization in 20min
The analysis of compound measures, in the case of ensureing separating degree, it is achieved that divide fast and effectively
Analysis, is conducive to controlling the quality of product.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the claim of the present invention is done the most in detail
Illustrate, but do not constitute any limitation of the invention, any in the claims in the present invention protection
The amendment of the limited number of time that scope is made, still within the claims of the present invention.
Involved in the present invention to percentage composition concentration, in addition to specified otherwise, solute is liquid
Be volumetric concentration, solute be solid be mass concentration.
Embodiment 1
In a kind of wet tissue of detection simultaneously, the method for various skin disinfectant content, is to use efficiently
Liquid chromatography measure simultaneously chlorhexidine gluconate in wet tissue, chlorhexidine acetate, 2,4,
4 "-three chloro-2 "-dihydroxy diphenyl ether, benzalkonium bromide, benzalkonium chloride and the content of glutaraldehyde.
Described method is specific as follows: include following step successively:
1) squeeze out liquid component in wet tissue, collect liquid；
2) step 1 is measured) obtain liquid 1ml, it is transferred in 50ml volumetric flask, adds
30% ethanol, supersound extraction 5min, extract 2 times, united extraction liquid is also evaporated, and adds methanol
It is settled to 50ml, filters 0.45um filter membrane, inject Agilant12000 high performance liquid chromatography
Instrument detects, and measures chromatogram；
3) weigh chlorhexidine gluconate, chlorhexidine acetate respectively, 2,4,4 "-three is chloro-
2 "-dihydroxy diphenyl ether, benzalkonium bromide, benzalkonium chloride and glutaraldehyde, adds methanol and makes into concentration and be
0.02mg/mL, detects material list mark chromatograph volume mixed chromatogram.
Chromatographic condition during above-mentioned detection is as follows:
Chromatographic column: Thermo AccliaimTM Mixed-Mode WCX-1 post；
Flowing phase: A: water；B:1mol/L Acetic acid-sodium acetate buffer, pH 6.0；C:
Methanol；
Gradient elution program: 0～1min, 3%～97%A, 2%B, 95%～1%C；1～
4min, 13%～87%A, 2%B, 85%～11%C；4～15.5min, 40%～31%A,
10%～19%B, 50%C；5min is balanced under 80%A, 15%B, 5%C；Flow velocity:
0.8mL/min；Column temperature: 35 DEG C；Sample size: 5 μ l；Detection wavelength: 258nm.
Above-mentioned testing result being contrasted with standard colour chart, its testing result is basically identical.
Taking the product continuous sample introduction six times of same lot number, its average recovery rate is 101.6%, RSD
It is 0.83%.
Taking the moisture paper tissue papers of three batches of different manufacturers, detect according to the method described above, it detects knot
Fruit is as follows:
Claims (4)
1. detecting a method for various skin disinfectant content in wet tissue, its feature exists simultaneously
In, it is to use chlorhexidine gluconate, acetic acid in hplc simultaneous determination wet tissue
Chlorhexidine, 2,4,4 "-three chloro-2 "-dihydroxy diphenyl ether, benzalkonium bromide, benzalkonium chloride and
The content of glutaraldehyde.
One the most according to claim 1 detects various skin disinfectant in wet tissue simultaneously
The method of content, it is characterised in that multiple in described high effective liquid chromatography for measuring wet tissue
Skin degerming agent content, includes following step successively:
1) squeeze out liquid component in wet tissue, collect liquid；
2) step 1 is measured) obtain liquid 1ml, it is transferred in 50ml volumetric flask, adds
30% ethanol, supersound extraction 5min, extract 2 times, united extraction liquid is also evaporated, and adds methanol
It is settled to 50ml, filters, inject high performance liquid chromatograph detection, measure chromatogram；
3) weigh chlorhexidine gluconate, chlorhexidine acetate respectively, 2,4,4 "-three is chloro-
2 "-dihydroxy diphenyl ether, benzalkonium bromide, benzalkonium chloride and glutaraldehyde, adds methanol and makes into concentration and be
0.02mg/mL, detects material list mark chromatograph volume mixed chromatogram.
One the most according to claim 2 detects various skin disinfectant in wet tissue simultaneously
The method of content, it is characterised in that described chromatographic condition is as follows:
Chromatographic column: Thermo AccliaimTM Mixed-Mode WCX-1 post；
Flowing phase: A: water；B:1mo l/L Acetic acid-sodium acetate buffer, pH 6.0；C:
Methanol；
Gradient elution program: 0～1min, 3%～97%A, 2%B, 95%～1%C；1～
4min, 13%～87%A, 2%B, 85%～11%C；4～15.5min, 40%～31%A,
10%～19%B, 50%C；5min is balanced under 80%A, 15%B, 5%C；Flow velocity:
0.8mL/min；Column temperature: 35 DEG C；Sample size: 5 μ l；Detection wavelength: 258nm.
One the most according to claim 2 detects various skin disinfectant in wet tissue simultaneously
The method of content, it is characterised in that described high performance liquid chromatograph is Agilant12000
High performance liquid chromatograph.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610259740.6A CN105954397A (en) | 2016-04-25 | 2016-04-25 | Method for simultaneously measuring contents of multiple skin disinfectants in wet tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610259740.6A CN105954397A (en) | 2016-04-25 | 2016-04-25 | Method for simultaneously measuring contents of multiple skin disinfectants in wet tissue |
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| CN105954397A true CN105954397A (en) | 2016-09-21 |
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| CN201610259740.6A Pending CN105954397A (en) | 2016-04-25 | 2016-04-25 | Method for simultaneously measuring contents of multiple skin disinfectants in wet tissue |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108362803A (en) * | 2018-01-24 | 2018-08-03 | 广东医科大学 | A kind of method of glutaraldehyde content in measurement material |
| CN108680688A (en) * | 2018-05-08 | 2018-10-19 | 江苏安舜技术服务有限公司 | The assay method of BAC and DDAC in a kind of soil and plant-derived product |
| CN109541069A (en) * | 2018-12-20 | 2019-03-29 | 上海开米科技有限公司 | The detection method of the chloro- 2- dihydroxy diphenyl ether content of 4,4- bis- in a kind of detergent |
| CN109633036A (en) * | 2019-01-29 | 2019-04-16 | 扬州倍加洁日化有限公司 | The test method of benzalkonium chloride content in a kind of wet tissue |
| CN110749659A (en) * | 2018-07-23 | 2020-02-04 | 佛山市南海东方澳龙制药有限公司 | Content detection method of chlorhexidine gluconate |
| CN113009009A (en) * | 2021-01-28 | 2021-06-22 | 山东省药学科学院 | Gas chromatography-mass spectrometry combined method for detecting residual quantity of glutaraldehyde in cefprozil |
| CN113984912A (en) * | 2021-09-02 | 2022-01-28 | 陕西新时代生物转化检测有限公司 | Method for detecting benzalkonium chloride in disinfection product |
| CN115494171A (en) * | 2022-09-20 | 2022-12-20 | 广州海关技术中心 | Method for detecting chlorhexidine and benzalkonium compounds in disinfectant |
Citations (3)
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| CN101285779A (en) * | 2007-04-12 | 2008-10-15 | 上海利康消毒高科技有限公司 | Determination method for chlorhexidine acetate in iodine formulations |
| CN102375034A (en) * | 2010-08-17 | 2012-03-14 | 贾明宏 | Guanidine-benzoin derivative reaction and application of guanidine-benzoin derivative reaction in rapid detection by high performance liquid chromatography |
| CN103081940A (en) * | 2013-02-05 | 2013-05-08 | 河南科技学院 | Compound chlorhexidine acetate microemulsion disinfectant and its preparation method |
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Patent Citations (3)
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| CN101285779A (en) * | 2007-04-12 | 2008-10-15 | 上海利康消毒高科技有限公司 | Determination method for chlorhexidine acetate in iodine formulations |
| CN102375034A (en) * | 2010-08-17 | 2012-03-14 | 贾明宏 | Guanidine-benzoin derivative reaction and application of guanidine-benzoin derivative reaction in rapid detection by high performance liquid chromatography |
| CN103081940A (en) * | 2013-02-05 | 2013-05-08 | 河南科技学院 | Compound chlorhexidine acetate microemulsion disinfectant and its preparation method |
Non-Patent Citations (2)
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| 杨艳伟 等: "高效液相色谱法同时测定消毒剂中9种酚类化合物", 《卫生研究》 * |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108362803A (en) * | 2018-01-24 | 2018-08-03 | 广东医科大学 | A kind of method of glutaraldehyde content in measurement material |
| CN108680688A (en) * | 2018-05-08 | 2018-10-19 | 江苏安舜技术服务有限公司 | The assay method of BAC and DDAC in a kind of soil and plant-derived product |
| CN110749659A (en) * | 2018-07-23 | 2020-02-04 | 佛山市南海东方澳龙制药有限公司 | Content detection method of chlorhexidine gluconate |
| CN109541069A (en) * | 2018-12-20 | 2019-03-29 | 上海开米科技有限公司 | The detection method of the chloro- 2- dihydroxy diphenyl ether content of 4,4- bis- in a kind of detergent |
| CN109633036A (en) * | 2019-01-29 | 2019-04-16 | 扬州倍加洁日化有限公司 | The test method of benzalkonium chloride content in a kind of wet tissue |
| CN113009009A (en) * | 2021-01-28 | 2021-06-22 | 山东省药学科学院 | Gas chromatography-mass spectrometry combined method for detecting residual quantity of glutaraldehyde in cefprozil |
| CN113984912A (en) * | 2021-09-02 | 2022-01-28 | 陕西新时代生物转化检测有限公司 | Method for detecting benzalkonium chloride in disinfection product |
| CN115494171A (en) * | 2022-09-20 | 2022-12-20 | 广州海关技术中心 | Method for detecting chlorhexidine and benzalkonium compounds in disinfectant |
| CN115494171B (en) * | 2022-09-20 | 2024-01-26 | 广州海关技术中心 | Method for detecting chlorhexidine and benzalkonium compounds in disinfectant |
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Application publication date: 20160921 |
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