CN105954346B - The preparation method of three-dimensional paper substrate electrochemistry ratiometer - Google Patents
The preparation method of three-dimensional paper substrate electrochemistry ratiometer Download PDFInfo
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- CN105954346B CN105954346B CN201610595195.8A CN201610595195A CN105954346B CN 105954346 B CN105954346 B CN 105954346B CN 201610595195 A CN201610595195 A CN 201610595195A CN 105954346 B CN105954346 B CN 105954346B
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Abstract
The invention discloses the application of a kind of preparation method and the three-dimensional paper substrate ratiometer of three-dimensional paper substrate electrochemistry ratiometer in zinc ion detection.By wax printing technique in paper chip preparation work area, and by screen printing technique, print three electrodes, and then to workspace functionalization, the dual signal amplification for realizing two kinds of electroactive materials of methylene blue and o-phenylenediamine, identifies analogue enztme chain using metal ion, is broken substrate chain, by electrochemical workstation, Zn is realized2+Highly sensitive, portable inspectiont.
Description
Technical field
The present invention relates to a kind of preparation method of three-dimensional paper substrate electrochemistry ratiometer, more specifically a kind of three-dimensional paper substrate
The structure of electrochemistry ratiometer laboratory technique platform.
Background technology
Water pollution is prevented, it is most important to the health of environmental protection and the mankind.Some inorganic pollutions, it is especially golden
Belong to ion, such as Pb2+, Mg2+, Zn2+, Hg2+Etc. being substance common in water pollution, but it is now widely used chromatography, glimmering
Expensive equipment needed for light detection method, and instrument is relatively cumbersome, is unfavorable for instant Site Detection.Therefore, it finds and establishes and is a kind of
The detection method of inexpensive, efficient rapid metal ion becomes one of the task of researcher.
Paper is widely used in the fields such as writing, printing, drawing, packaging as one of four invents by China product.It is many
The paper fiber of traversed by mistake so that three-dimensional porous structure is presented inside paper, and paper fiber surface is rich in abundant oxygen-containing group, therefore,
It is easily achieved the surface-functionalized of paper fiber.In recent years, functionalization paper substrate experiment detection platform scale increasingly expands, by simple
Medical instrument tests pregnant stick and develops to miscellaneous biosensing device, including paper substrate electrochemica biological sensor, paper substrate photoelectricity
Chemical biosensor, paper substrate colorimetric bio sensor, paper substrate electrochemical luminescence biosensor etc..
Electrochemica biological sensor, because it has low cost, high sensitivity, it is easy to accomplish minimize and integrated, and institute
The advantages that needing instrument simple and be widely used in bio-sensing field.But traditional electrochemica biological sensor is inconvenient to carry, and
It is easy to be influenced by external environment, variation, H such as electrolyte pH2O2The change etc. of concentration, and make measurement result accuracy
It reduces.In order to solve this problem, the thought of Ratio-type fluorescence detection method is introduced into electrochemical biosensor by us for the first time
In device, binding function paper chip, we construct a kind of novel paper substrate electrochemistry ratiometer detection platform, constructed life
Object sensing platform not only have many advantages, such as it is inexpensive, foldable, be easy to carry about with one, while testing result sensitivity and accuracy obtain
It must be greatly improved, therefore, constructed laboratory technique platform is expected on the side that resource is limited, economic development is relatively backward
Remote area is applied and promotes.
Invention content
For presently, there are the problem of, the present invention has made a kind of three-dimensional paper at low cost, accuracy is high and easy to carry
Based electrochemical ratiometer simultaneously applies it to Zn2+Detection in.
In order to solve the above-mentioned technical problem, the present invention is realized by following measures：A kind of three-dimensional paper substrate electrochemistry
The preparation of ratiometer, it is characterized in that including the following steps：
(1) hydrophobic wax batch of AI Software for Design Ratio-type electrochemistry paper chip as shown in Fig. 1 is utilized on computers
Measure print pattern；
(2) Ratio-type electrochemistry paper chip is cut into the paper of A4 sizes, will be designed in step (1) using wax printer
Hydrophobic wax bulk print pattern print on A4 paper, be then placed into baking oven heating until wax melts and is impregnated with entirely
The thickness of paper forms hydrophobic region；
(3) method for using silk-screen printing, by working electrode, reference electrode, is printed onto step successively to electrode print pattern
Suddenly in (2) on gained paper, pattern is as shown in Fig. 2, wherein right side is workspace, is printed on working electrode above, left side is reference
Area is printed on reference electrode and to electrode above；
(4) right（3）Middle hydrophilic region functionalization will be modified with Au nano-particles-ferriporphyrin metal-organic framework material
Zn2+Specific DNA analogue enztme chain, be defined as chain 1, base sequence is fixed on hydrophilic region as shown in nucleotides sequence list,
The first time amplification for realizing signal, then blocks active site with sulfydryls hexanol；
(5) Zn for modifying certain density methylene blue2+Substrate chain, be defined as chain 2, base sequence such as nucleosides
Shown in acid sequence table, wherein to from left to right, the tenth base, that is, base A represent Adenosine acid, and are rolled
The primer strand of circle amplification is defined as chain 3, and base sequence is mixed in a certain ratio together, so as shown in nucleotides sequence list
After be added to（4）3 h are reacted at 43 DEG C of the electrode obtained surface；
(6) by certain density annular template probe, be defined as chain 4, base sequence as shown in nucleotides sequence list,
And T4 ligases are added to step（5）2 h are reacted on the electrode obtained surface at room temperature, subsequent addition phi29 DNA ligases,
The mixed liquor of dNTP and rolling circle amplification buffer solution react 1 h at 37 DEG C, then rinse electrode with phosphate buffer solution
Rolling circle amplification reaction is completed on surface, to realize that secondary singal is amplified；
(7) by certain density Zn2+It is added to step（6）The electrode obtained surface, mixing react 40 min at room temperature；
(8) to step（7）The electrode obtained surface, which is added dropwise, contains o-phenylenediamine and H2O2Phosphate buffer solution electrolysis
Liquid connects electrochemical workstation, in -0.8 ~ 0 V potential ranges, measures the current strength of methylene blue and o-phenylenediamine；
(9) variation and the Zn of o-phenylenediamine and methylene blue current strength ratio are drawn2+Concentration relationship curve completes Zn2 +Measurement；
Step（2）Described in paper material, it is characterised in that its be level-one chromatographic paper；
Step（4）Described in hydrophilic region functionalization, it is characterised in that：Graphene conductive layer is grown in paper chip, and
After chitosan solution is added dropwise：Repeating to be added dropwise the solution of graphene oxide three times to paper chip surface will be added dropwise after drying at room temperature
There is the paper chip of graphene oxide to be transferred to containing 0.1 M Na2SO4In solution, under 0.9 V current potentials, electroreduction graphene oxide,
Paper chip is taken out after the completion of reaction, secondary water cleans surface, dry at room temperature again to can be obtained the paper for growing and having graphene
The 0.5 mg mL of 2.0 μ L are then added dropwise in chip on paper chip surface-1Chitosan, at room temperature dry 40 min.
Step（4）Described in be modified with the Zn of Au nano-particles-ferriporphyrin metal-organic framework material2+Specific DNA mould
Quasi- enzyme chain, it is characterised in that：Prepare ferriporphyrin metal-organic framework material, i.e., the 5 of 39.54 mg first, 10,15,20- tetra-（Four
Carboxyl）The hydrochloric acid of -21H, 23H- porphyrin and 40.55 mg iron chloride, 0.1 M is added to the volume ratio 1 of 12 mL:The two of 2 mixing
In the mixed liquor of methylformamide and ethyl alcohol, 40 min of ultrasound, then by 150 DEG C of 50 h of reaction of mixed liquor, reaction will after stopping
Product dimethylformamide and ethyl alcohol clean three times, then by 60 DEG C of vacuum drying of product, you can obtaining ferriporphyrin metal has
Machine frame frame material；Secondly Au nano-particles-ferriporphyrin metal-organic framework material is prepared：Weigh the iron porphin of the above-mentioned preparation of 6 mg
Quinoline metal-organic framework material is dissolved into the ethyl alcohol of 1 mL, and ammonium hydroxide, 13.8 mL of 0.2 mL are then dispersed in
In ethyl alcohol, 1.8 mL tetramethoxy-silicane mixed liquors, 2 h are stirred at room temperature, and product is centrifuged into 10 min in 12000 r/min,
With ethyl alcohol wash products, by the ethanol solution of the trimethoxy mercapto propyl silane of 20 mM of the product obtained and 4 mL, 65
DEG C reflux 4 h, product is centrifuged, secondary water rinse, the solution of 4 mL Au nano-particles is then added into solution, at room temperature
3 h are reacted, product is centrifuged, are rinsed with secondary water, you can obtain Au nano-particles-ferriporphyrin metal-organic framework material；Most
Afterwards by Zn2+Specific DNA analogue enztme chain link is on Au nano-particles-ferriporphyrin metal-organic framework material surface：Pipette 90 μ L
10 μM of Zn2+Specific DNA analogue enztme chain is added in Au nano-particles-ferriporphyrin metal-organic framework material solution, room
Temperature is lower to shake 24 h, and the product obtained blocks active site with sulfydryls hexanol, product is finally distributed to 0.5 mL, 50 mM
Three (methylol) aminomethane acetate buffer solutions in.
Chain 1 described in step (4), it is characterised in that:Its specific base sequence and its 5 ' terminal modified upper amino,
3 ' terminal modified upper sulfydryls.
DNA chain 4 described in step (6), it is characterised in that：Its specific base sequence and its 5 ' terminal modified upper phosphate
Group.
Beneficial effects of the present invention
（1）Using Ratio-type electrochemical method to Zn2+It is detected so that measurement result accuracy improves.
（2）For functionalization paper chip as Electrochemical Detection substrate, active site is more, is easy to the characteristics of batch production.
（3）Entire Ratio-type paper substrate electrochemical device preparation process is simple, and required expense is low, is expected to realize Zn2+It is low at
Originally, portable inspectiont.
Description of the drawings
Fig. 1：The hydrophobic wax bulk print pattern of three-dimensional paper chip；
Fig. 2：Working electrode, reference electrode and to electrode in silk-screen printing on hydrophobic wax print pattern, wherein right side is
Working electrode, left side is for reference and to electrode.
Specific implementation mode
For a better understanding of the present invention, below by the embodiment content that the present invention is furture elucidated, but the present invention is not
It is limited only to the following examples.
Embodiment 1
The preparation of a kind of three-dimensional paper substrate electrochemistry ratiometer and its in Zn2+Application in detection：
(1) the hydrophobic wax bulk print pattern of AI Software for Design Ratio-type electrochemistry paper chips is utilized on computers；
(2) Ratio-type electrochemistry paper chip is cut into the paper of A4 sizes, will be designed in step (1) using wax printer
Hydrophobic wax bulk print pattern print on A4 paper, be then placed into baking oven heating until wax melts and is impregnated with entirely
The thickness of paper forms hydrophobic region；
(3) method for using silk-screen printing, by working electrode, reference electrode, is printed onto step successively to electrode print pattern
Suddenly, wherein right side is workspace, it is printed on working electrode above on gained paper in (2), left side is reference region, is printed on reference electricity above
Pole and to electrode；
(4) right（3）Middle hydrophilic region functionalization will be modified with Au nano-particles-ferriporphyrin metal-organic framework material
Zn2+Specific DNA analogue enztme chain, be defined as chain 1, be fixed on hydrophilic region, then with sulfydryls hexanol block active site；
(5) Zn for modifying certain density methylene blue2+Substrate chain, be defined as chain 2, wherein to from left to right, the tenth
A base, that is, base A represent Adenosine acid and primer strand, are defined as chain 3, are mixed in a certain ratio one
It rises, 3 h is reacted at 43 DEG C, are then added to（4）The electrode obtained surface；
(6) it by certain density annular template probe, is defined as chain 4 and T4 ligases is added to step（5）Gained
Electrode surface reacts 2 h at room temperature, and the mixing of phi29 DNA ligase, dNTP and rolling circle amplification buffer solution is then added
Liquid reacts 1 h at 37 DEG C, then rinses electrode surface with phosphate buffer solution；
(7) by certain density Zn2+It is added to step（6）In gained mixed liquor, mixing reacts 40 min at room temperature；
(8) to step（7）The electrode obtained surface, which is added dropwise, contains o-phenylenediamine and H2O2Phosphate buffer solution electrolysis
Liquid connects electrochemical workstation, in -0.8 ~ 0 V potential ranges, measures the current strength of methylene blue and o-phenylenediamine；
(9) variation and the Zn of o-phenylenediamine and methylene blue current strength ratio are drawn2+Concentration relationship curve completes Zn2 +Measurement.
SEQUENCE LISTING
<110>University Of Ji'nan
<120>The preparation method of three-dimensional paper substrate electrochemistry ratiometer
<130> 1
<160> 4
<170> PatentIn version 3.3
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<211> 51
<212> DNA
<213>Artificial sequence
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catctcttct ccgagccggt cgaaatagtg agtcatgcat gcatgtacat c 51
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<212> DNA
<213>Artificial sequence
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actcactata ggaagagatg 20
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acagagccta tagagctgat gatgtacatg catgca 36
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<212> DNA
<213>Artificial sequence
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atctcgacta aaaacccaac ccgccctacc caaaatgtct cggat 45
Claims (4)
1. the preparation method of three-dimensional paper substrate electrochemistry ratiometer, it is characterized in that including the following steps：
(1) the hydrophobic wax bulk print pattern of AI Software for Design Ratio-type electrochemistry paper chips is utilized on computers；
(2) Ratio-type electrochemistry paper chip is cut into the paper of A4 sizes, is dredged what is designed in step (1) using wax printer
Water wax bulk print pattern prints on A4 paper, is then placed into heating in baking oven until wax melts and is impregnated with entire paper
Thickness forms hydrophobic region；
(3) method for using silk-screen printing, by working electrode, reference electrode, is printed onto step successively to electrode print pattern
(2) on gained paper, wherein right side is workspace, it is printed on working electrode above, left side is reference region, is printed on reference electrode above
With to electrode；
(4) to step（3）Middle hydrophilic region functionalization will be modified with Au nano-particles-ferriporphyrin metal-organic framework material
Zn2+Specific DNA analogue enztme chain, be defined as chain 1, be fixed on hydrophilic region, realize signal first time amplification, then use mercapto
Base hexanol blocks active site；
(5) Zn for modifying certain density methylene blue2+Substrate chain, be defined as chain 2, wherein to from left to right, the tenth alkali
Base, that is, base A represent Adenosine acid, and carry out the primer strand of rolling circle amplification, are defined as chain 3, by certain ratio
Example mixes, and is then added to step（4）3 h are reacted under 43 °C of the electrode obtained surface；
(6) it by certain density annular template probe, is defined as chain 4 and T4 ligases is added to step（5）The electrode obtained
2 h are reacted on surface at room temperature, and the mixed liquor of phi29 DNA ligases, dNTP and rolling circle amplification buffer solution is then added,
1 h is reacted under 37 °C, then rinses electrode surface with phosphate buffer solution, rolling circle amplification reaction is completed, to realize secondary letter
Number amplification；
(7) by certain density Zn2+It is added to step（6）The electrode obtained surface, mixing react 40 min at room temperature；
(8) to step（7）The electrode obtained surface, which is added dropwise, contains o-phenylenediamine and H2O2Phosphate buffer solution electrolyte, even
Electrochemical workstation is connect, in -0.8 ~ 0 V potential ranges, measures the current strength of methylene blue and o-phenylenediamine；
(9) variation and the Zn of o-phenylenediamine and methylene blue current strength ratio are drawn2+Concentration relationship curve completes Zn2+Survey
It is fixed；
Step（2）Described in A4 paper, it is characterised in that its be level-one chromatographic paper；
Step（4）Described in hydrophilic region functionalization, it is characterised in that：Graphene conductive layer is grown in paper chip, is then dripped
Add chitosan solution：Repeating to be added dropwise the solution of graphene oxide three times to paper chip surface will be added dropwise aerobic after drying at room temperature
The paper chip of graphite alkene is transferred to the Na of a concentration of 0.1 M2SO4In solution, under 0.9 V current potentials, electroreduction graphene oxide,
Paper chip is taken out after the completion of reaction, secondary water cleans surface, dry at room temperature again to can be obtained the paper for growing and having graphene
The 0.5 mg mL of 2.0 μ L are then added dropwise in chip on paper chip surface-1Chitosan, at room temperature dry 40 min.
2. according to the preparation method of the three-dimensional paper substrate electrochemistry ratiometer described in claim 1, it is characterised in that：Claim
1 step（4）Described in be modified with the Zn of Au nano-particles-ferriporphyrin metal-organic framework material2+Specific DNA analogue enztme chain,
It is characterized in that：Prepare ferriporphyrin metal-organic framework material, i.e., the 5 of 39.54 mg first, 10,15,20- tetra-（Tetracarboxylic）-
The hydrochloric acid of 21H, 23H- porphyrin and 40.55 mg iron chloride, 0.1 M is added to the volume ratio 1 of 12 mL:The dimethyl methyl of 2 mixing
In the mixed liquor of amide and ethyl alcohol, 40 min of ultrasound, then by 150 °C of 50 h of reaction of mixed liquor, reaction uses product after stopping
Dimethylformamide and ethyl alcohol clean three times, then by 60 °C of vacuum drying of product, you can obtain ferriporphyrin metal organic frame
Material；Secondly Au nano-particles-ferriporphyrin metal-organic framework material is prepared：Weigh the ferriporphyrin metal of the above-mentioned preparation of 6 mg
Organic framework materials are dissolved into the ethyl alcohol of 1 mL, be then dispersed in the ammonium hydroxide of 0.2 mL, 13.8 mL ethyl alcohol,
In 1.8 mL tetramethoxy-silicane mixed liquors, 2 h are stirred at room temperature, and product is centrifuged into 10 min in 12000 r/min, uses ethyl alcohol
Wash products mix the product obtained with the ethanol solution of the trimethoxy mercapto propyl silane of 4 mL, 20 mM, and 65 °C are returned
4 h are flowed, product is centrifuged, secondary water is rinsed, and the solution of 4 mL Au nano-particles is then added into solution, reacts 3 at room temperature
H centrifuges product, is rinsed with secondary water, you can obtains Au nano-particles-ferriporphyrin metal-organic framework material；Finally by Zn2+
Specific DNA analogue enztme chain link is on Au nano-particles-ferriporphyrin metal-organic framework material surface：Pipette 10 μM of 90 μ L
Zn2+Specific DNA analogue enztme chain is added in Au nano-particles-ferriporphyrin metal-organic framework material solution, shakes at room temperature
24 h are swung, the product obtained blocks active site with sulfydryls hexanol, and product is finally distributed to the three (hydroxyls of 0.5 mL, 50 mM
Methyl) in aminomethane acetate buffer solution.
3. according to the preparation method of the three-dimensional paper substrate electrochemistry ratiometer described in claim 1, it is characterised in that：Claim
Chain 1 described in 1 step (4), it is characterised in that:Its specific base sequence and its 5 ' terminal modified upper amino, 3 ' is terminal modified
Upper sulfydryl.
4. according to the preparation method of the three-dimensional paper substrate electrochemistry ratiometer described in claim 1, it is characterised in that：Claim
Chain 4 described in 1 step (6), it is characterised in that：Its specific base sequence and its 5 ' terminal modified upper phosphate group.
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| CN106323934B (en) * | 2016-11-18 | 2019-06-14 | 重庆工商大学 | It is a kind of to detect Cu simultaneously2+、Mg2+And Pb2+The fluorescent bio-probes and its detection method of three kinds of ions |
| CN107024523A (en) * | 2017-04-25 | 2017-08-08 | 济南大学 | A kind of preparation and application of paper substrate optical electro-chemistry molecular engram sensor |
| CN107236790A (en) * | 2017-05-09 | 2017-10-10 | 济南大学 | A kind of paper substrate Sensitive Detection miRNA method |
| CN107393980B (en) * | 2017-08-28 | 2019-01-15 | 济南大学 | A kind of preparation method of paper base graphene-p-type cuprous oxide composite material |
| CN107727714B (en) * | 2017-09-10 | 2019-06-28 | 福建师范大学 | One kind being based on carbon nanohorn and TiO2The preparation method of the Ratio-type electrochemical luminescence immunosensor of mesomorphic nano material |
| CN107860805B (en) * | 2017-11-03 | 2019-08-30 | 青岛大学 | A kind of preparation method of ratio electrochemistry dopamine aptamer sensor |
| CN110487871A (en) * | 2019-08-28 | 2019-11-22 | 济南大学 | A kind of preparation of electrochemistry Ratio-type paper chip and its application in detection of nucleic acids |
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| CN104122393B (en) * | 2014-07-31 | 2015-12-30 | 济南大学 | A kind of preparation of Photoelectrochemicalthree three-dimensional paper chip and the application in lesion detection thereof |
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