CN105950506A - Bacillus amyloliquefaciens for preventing and curing tomato bacterial wilt and application of bacillus amyloliquefaciens - Google Patents
Bacillus amyloliquefaciens for preventing and curing tomato bacterial wilt and application of bacillus amyloliquefaciens Download PDFInfo
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Abstract
The invention relates to bacillus amyloliquefaciens W118. The bacillus amyloliquefaciens W118 is collected in China General Microbiological Culture Collection Center (CGMCC) on March 10, 2016, and the collection number is CGMCC No.12200. The invention further provides application of the bacillus amyloliquefaciens W118 in prevention and cure of pathogenic bacteria of tomato bacterial wilt, cotton verticillium wilt, tomato early blight and cucumber corynespora cassiicola. Compared with the prior art, the bacillus amyloliquefaciens W118 and the application thereof have the advantages that the bacillus amyloliquefaciens W118 has a certain prevention and cure effect on the tomato bacterial wilt, and the highest prevention and cure rate can reach 59.2%; the highest prevention and cure rates for the cotton verticillium wilt, the tomato early blight and the cucumber corynespora cassiicola can reach 47.8%, 43.4% and 38.3% respectively.
Description
Technical field
The invention belongs to crops preventing and curing diseases technical field, particularly relate to a strain for the bacillus amyloliquefaciens of control of plant bacterial wilt and application thereof.
Background technology
Bacterial wilt of tomato is that the destructive soil of the one caused by Ralstonia solanacearum (Ralstonia solanacearum) passes bacteriosis, it is dead that this disease can cause plant large area to wilt, the sickness rate in severe disease field reaches more than 80%, cause tomato yield degradation, seriously govern the development of Fructus Lycopersici esculenti industry and the raising of economic benefit.Ralstonia solanacearum fungus strain is complicated, and host is extensive, and under hot and humid environment, vitality is indomitable, can infect 50 Duo Ge section 450 various plants.
The method of the most conventional control of plant bacterial wilt mainly has: chemical prevention, improvement soil, breeding resistant variety, grafting and crop rotation etc., but effect is the most unstable[3-4].Additionally, the use of chemical agent can bring the series of problems such as pollution and the food safety of environment, therefore Biological control be can yet be regarded as an environmental protection, economy, effectively preventing approach.Have become as the prior development direction preventing and treating this disease.
Biological control is mainly by antagonistic microbe and metabolite controlling plant diseases thereof.At present, it has been reported that mainly include bacillus (Bacillus spp), pseudomonas (Pseudomonas spp) and streptomyces (Streptomyces spp) etc. for the antagonistic bacterium preventing and treating bacterial wilt.Wherein bacillus is due to its fast growth, high temperature resistant, radiation hardness, resistance to high soda acid, and can produce multiple antagonism class material, is the emphasis in antagonistic bacterium research.
Plant Growth-promoting Rhizobacteria (PGPR) refers to the class beneficial microbe lived near plant rhizosphere, this quasi-microorganism often has antagonism pathogenic microorganism, molten phosphorus effect, nitrogen fixation, enhancing plant disease-resistant, strengthens the effects such as alimentation, therefore, the PGPR bacterial strain that separation screening obtains at plant rhizosphere has great importance.
Bacillus amyloliquefaciens is the quasi-microorganism being active in plant rhizosphere, the advantage that existing bacillus is had, belong to again the big class of PGPR mono-, the anti-disease mechanism of bacillus amyloliquefaciens mainly has the following aspects: first, bacterial strain is by producing active substance or improving mineral matter nutritional promotion vegetation growth of plant, and indirect help plant resists infecting of pest and disease damage；Second, bacterial strain is produced metabolite or is suppressed or hinder the development of rhizosphere pathogenic microorganism by Competition, indirectly promote the growth of plant, its Biocontrol Effect mechanism mainly includes the root colonization of effect, antibiosis, rhizosphere nutrition competition (the particularly competition to ferrum), the enzyme of secretion degrading microorganism and inducing plant resistance etc..
Summary of the invention
One to be solved by this invention technical problem is that the present situation for prior art provides a kind of bacillus amyloliquefaciens W118, it is that a kind of healthy rhizosphere soil serious from bacterial wilt of tomato morbidity separates the biocontrol bacteria bacterial strain to bacterial wilt of tomato with antagonism, can effectively suppress the flourish of bacterial wilt of tomato pathogen, the preventing and treating to bacterial wilt of tomato is significant.
To be solved by this invention another technical problem is that the present situation for prior art provides the application of bacillus amyloliquefaciens W118.
The present invention solves the technical scheme that above-mentioned technical problem used: this bacillus amyloliquefaciens W118, is preserved in China General Microbiological culture presevation administrative center (CGMCC) on March 10th, 2016, and deposit number is CGMCC No.12200.
The present invention also provides for the application of above-mentioned bacillus amyloliquefaciens W118, it is characterised in that the application to control of plant bacterial wilt.
And the application of above-mentioned bacillus amyloliquefaciens W118, be also embodied in: to preventing and treating cotton verticillium wilt, early blight of tomato and Leaf Spot Caused by Corynespora cassiicola on Cucumber are prevented and treated in application.
Above-mentioned bacillus amyloliquefaciens W118 application in preparing biocontrol agent, it is characterised in that: the effective dosage ranges of described anti-microbial inoculum is 1.0 × 107～1.0 × 109cfu/ml.。
Above-mentioned bacillus amyloliquefaciens W118 application in preparing biocontrol agent, it is characterised in that: the effective dosage ranges of described anti-microbial inoculum is 1.0 × 108～1.0 × 109cfu/ml、1.0×107～1.0 × 109cfu/ml、1.0×107～1.0 × 109cfu/ml。
Compared with prior art, present invention have the advantage that field test shows, use bacillus amyloliquefaciens W118 Biological agents to have a certain degree of prevention effect to bacterial wilt of tomato, prevention effect is up to 59.2%；Prevention effect to other disease: cotton verticillium wilt, early blight of tomato, the prevention effect best result of Leaf Spot Caused by Corynespora cassiicola on Cucumber are not reached 47.8%, 43.4%, 38.3%.
Preservation explanation
1, bacillus amyloliquefaciens W118, Classification And Nomenclature is: bacillus amyloliquefaciens, Bacillus amyloliquefaciens is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) on March 10th, 2016, deposit number is CGMCC No.12200, depositary institution address: Chaoyang District Beijing North Star West Road 1 institute 3.
Accompanying drawing explanation
Fig. 1 is the colonial morphology of bacillus amyloliquefaciens W118 in the present invention；
Fig. 2 is the bacillus amyloliquefaciens W118 flat board antagonism to bacterial wilt of tomato pathogen in the present invention；
Fig. 3 is the bacillus amyloliquefaciens W118 flat board antagonism to cotton verticillium wilt pathogen in the present invention；
Fig. 4 is the bacillus amyloliquefaciens W118 flat board antagonism to early blight of tomato pathogen in the present invention；
Fig. 5 is the bacillus amyloliquefaciens W118 flat board antagonism to Leaf Spot Caused by Corynespora cassiicola on Cucumber pathogen in the present invention.
Detailed description of the invention
Below by way of combining drawings and Examples, the invention will be further described.
The acquisition of the biocontrol bacterial strain W118 of embodiment 1 control of plant bacterial wilt
The screening of bacillus amyloliquefaciens with separate
(1) soil sample collection is from the healthy plant Rhizosphere Soil in the serious land for growing field crops of bacterial wilt of tomato morbidity, is uprooped by healthy plant, shakes gently, after major part soil is shaken off, residue is close to the soil of root and is collected together with part fibrous root, installs with sealed bag, puts into 4 DEG C of Refrigerator stores standby.
(2) being equipped with in the triangular flask of 90mL sterilized water by 10g Fructus Lycopersici esculenti healthy plant Rhizosphere Soil, acutely vibrate on the shaking table of 30 DEG C 30min, stands 30min；
(3) taking supernatant serial dilution and coat in beef extract-peptone improved culture medium, each gradient repeats, after 30 DEG C of constant temperature culture 24h, with equipped with 1.0 × 10 for 3 times8It is sprayed bacterium by the laryngeal spray of the Ralstonia solanacearum bacterium solution of cfu/ml；
(4) occur after obvious inhibition zone until flat board, select inhibition zone and the different bacterium colony of form is purified；
(5) take the antibacterial after three primary dcreening operations of purification, NA flat board carries out the multiple sieve that stands facing each other.Cultivate 12h, with 1.0 × 10 for 30 DEG C8The bacterial wilt original bacteria liquid hydrojet of cfu/ml, measures antibacterial circle diameter, the antibacterial circle diameter bacterial strain more than 1.5cm, saves backup after 48h.
The qualification of the biocontrol bacterial strain W118 of embodiment 2 control of plant bacterial wilt
Bacterial strain W118 is inoculated in beef extract-peptone improved culture medium, cultivates 48h for 30 DEG C, extract the genomic DNA of antagonistic strain with bacterial genomes DNA extraction kit, use bacterial 16 S rRNA primer to expand.(F:5 '-AGAGTTTGATCCTGGCTCAG-3 ' R:5 '-TACCTTGTTACGACTT-3 ').Reaction system is: Mg2+2.5 μ l, 10 × buffer 2.5 μ l, dNTP (10mmol L-1) 2 μ l, upstream and downstream primer (10mmol L-1) each l μ l, template DNA 1 μ l, Taq enzyme (5U μ l-1) 0.3 μ l, ddH2O 14.7 μ l, total system is 25 μ l.Agents useful for same is purchased from Dalian treasured biological engineering company limited.
PCR reaction condition: 95 DEG C of denaturations 5min, 95 DEG C of degeneration 30s, 53 DEG C of annealing lmin, 72 DEG C extend 2min, and 72 DEG C keep 10min, altogether circulation 30 times.The PCR primer agarose gel electrophoresis of 1% (w/v) detects, voltage 90V, and 15min, EB dye 30min, find target stripe.The purification of pcr amplification product uses the gel purification kit of Axygen company, and description is shown in concrete operations.The PCR primer of purification saves backup in-20 DEG C.
Selecting the carrier T of Takara company to connect with the 16S rRNA genetic fragment enzyme of amplification, reaction system is l0 μ l:DNA fragment 5 μ l, and pMD19-T carrier 0.3 μ l, Solution I solution 5 μ l, 16 DEG C overnight.Connection is had in the plasmid transformation escherichia coli competent cell of purpose fragment, carry out blue white macula screening, positive colony is sent to Shanghai Major Biological Medical Technology Co., Ltd.'s order-checking and identifies.
According to the sequencing result of 16S rRNA gene,http://www.ncbi.nlm.nih.govOnline query is analyzed, and utilizes the Blast software 16S rRNA gene order with other bacterial strain in GenBank to carry out tetraploid rice, selects close sequence and the sequence MEGA5.0 software building 16S rRNA phylogenetic analysis of order-checking bacterial strain.Result shows, the 16S rDNA sequence of bacterial strain W118 is with 100 bacillus of other comparisons, to belong to homology similarity the highest, and with bacillus amyloliquefaciens (B.amyloliquefaciens DSM7, NC014551) homology is the highest, it is 99%, identifies that W118 is bacillus amyloliquefaciens.
The embodiment 3 bacillus amyloliquefaciens W118 antagonism to bacterial wilt of tomato pathogen
Beef extract-peptone improved culture medium meets bacterial strain W118 after purification with sterile toothpick point, cultivates 12h, with 1.0 × 10 for 30 DEG C8The bacterial wilt original bacteria liquid hydrojet of cfu/m, measures antibacterial circle diameter, is repeated 3 times after 48h, observe inhibition zone size, stronger to bacterial wilt of tomato bacterium inhibitory action by bacillus amyloliquefaciens W118 seen from attached Fig. 1 and 2.
The embodiment 4 bacillus amyloliquefaciens W118 antagonism to other plant disease pathogen
Use flat board face-off method, the test bacillus amyloliquefaciens W118 antagonism to the pathogen of cotton verticillium wilt (Verticillium dahliae Kleb.), early blight of tomato (Alternaria Nees) and Leaf Spot Caused by Corynespora cassiicola on Cucumber (Corynespora) these three disease, by pathogen 28 DEG C of cultivation 7d on PDA plate, beat on flat board with the card punch of a diameter of 5mm and take circular agar block.The pathogen bacterium cake of the plant disease of a diameter of 5mm is accessed PDA plate central authorities, cultivate 2d for 28 DEG C, then, with sterilizing toothpick equidistant point inoculation antagonistic bacterium W118 to be measured around pathogen bacterium cake, in incubator 28 DEG C cultivate 5d after the antibacterial circle diameter that has that it's too late of observed and recorded inhibition zone.Each process is repeated 3 times.
Bacillus amyloliquefaciens W118 antimicrobial spectrum is wider, cotton verticillium wilt, early blight of tomato, Leaf Spot Caused by Corynespora cassiicola on Cucumber had antagonism, specifically it is shown in Table 1 and accompanying drawing 3,4 and 5, from accompanying drawing 3, can be seen that bacillus amyloliquefaciens W118 has antagonism to cotton verticillium wilt pathogen；From accompanying drawing 4, can be seen that bacillus amyloliquefaciens W118 bacterium former to early blight of tomato has antagonism；From accompanying drawing 5, can be seen that bacillus amyloliquefaciens W118 has antagonism to Leaf Spot Caused by Corynespora cassiicola on Cucumber pathogen.
The table 1 bacillus amyloliquefaciens W118 flat board antagonism to multiple pathogen
The embodiment 5 bacillus amyloliquefaciens W118 fermentation liquid field control effect to bacterial wilt of tomato
Field test is carried out in Heng Xi base, Yinzhou District, Ningbo City, and test material is bacterial strain W118 fermentation liquid, and test arranges 1.0 × 106cfu/ml、1.0×107cfu/ml、1.0×108cfu/ml、1.0×1094 Concentraton gradient of cfu/ml, transplant after 1 month in tomato seedling and start to use the fermentation liquid of antagonistic strain, use 1 time every 7d, 3 times altogether.Every strain tomato seedling pouring 10ml, often processes 3 times and repeats, to use equivalent clear water for comparison.
Disease investigation: after pouring bacterium, 7d investigates and record the incidence of each process tomato plant every time, adds up disease index and the prevention effect of each process.According to plant leaf wilting degree, morbidity progression being divided into 5 grades: 0 grade, plant is normal；1 grade, plant leaf wilting≤25%；2 grades, 26%≤plant leaf wilting≤50%；3 grades, 51%≤plant leaf wilting≤75%；4 grades, 76%≤plant leaf is wilted or dead.
Disease index=Σ (sick progression × this disease level plant strain number)/(serious disease progression × total strain number) × 100
Prevention effect (%)=(comparison disease index-process disease index)/comparison disease index × 100
The table 2 bacillus amyloliquefaciens W118 fermentation liquid prevention effect to bacterial wilt of tomato
1) mean+SD.In same column, different lower cases represent notable at 0.05 level difference, alphabetical identical expression zero difference, and letter difference then represents variant.
It can be seen that W118 fermentation liquid is as the rising of W118 fermentation liquid concentration to the prevention effect of bacterial wilt of tomato and improves from upper table 2, and concentration is in 1.0 × 109Prevention effect after cfu/ml fills bacterium at 3 times is best, 1.0 × 107cfu/ml、1.0×108cfu/ml、1.0×109In cfu/ml these three concentration, prevention effect diversity is not notable, but 1.0 × 106Cfu/ml prevention effect in above-mentioned 4 Concentraton gradient is relatively poor, and fills the prevention effect after bacterium and other concentration process significant difference for 3 times, therefore, it is suggested that recommended density is 1.0 × 107～1.0 × 109Cfu/ml, the prevention effect that its 3 times fill after bacterium is 46.7%～59.2%.
The embodiment 6 bacillus amyloliquefaciens W118 fermentation liquid field control effect to cotton verticillium wilt
Field test is carried out on Cixi City long river, Ningbo City town peasant household field, and test material is bacillus amyloliquefaciens W118 fermentation liquid, and test arranges 1.0 × 106cfu/ml、1.0×107cfu/ml、1.0×108cfu/ml、1.0×1094 Concentraton gradient of cfu/ml, transplant after 1 month in cotton seedling and use bacterial strain fermentation liquor, use 1 time every 7d, 3 times altogether.Every strain cotton seedling pouring 10ml, often processes 3 times and repeats, to use equivalent clear water for comparison.
Disease investigation: water bacterium 7d " Invest, Then Investigate " every time and record the incidence of each process cotton plants, adding up disease index and the prevention effect of each process.Cotton verticillium wilt grade scale is: 0 grade: cotton plant is healthy, anosis leaf, and growth is normal；1 grade: cotton plant incidence of leaf flavescence wilting≤25%；2 grades: 26%≤cotton plant incidence of leaf flavescence wilting≤50%；3 grades: 51%≤cotton plant incidence of leaf flavescence wilting≤75%, turn yellow and wilt；4 grades: cotton plant incidence of leaf turns yellow and wilts more than or equal to 76%.
Disease index=Σ (sick progression × this disease level plant strain number)/(serious disease progression × total strain number) × 100
Prevention effect (%)=(comparison disease index-process disease index)/comparison disease index × 100
The table 3 bacillus amyloliquefaciens W118 fermentation liquid prevention effect to cotton verticillium wilt
1) mean+SD.In same column, different lower cases represent notable at 0.05 level difference, alphabetical identical expression zero difference, and letter difference then represents variant.
As shown in Table 3, W118 fermentation liquid to the prevention effect of cotton verticillium wilt in concentration be 1.0 × 109Prevention effect after cfu/ml fills bacterium at 3 times is best, wherein, is 1.0 × 10 in concentration8Cfu/ml and 1.0 × 109Under cfu/ml, the prevention effect that 3 times fill after bacterium is best, and processes significant difference with other concentration.Therefore, it is suggested that recommending concentration is 1.0 × 108～1.0 × 109Cfu/ml, the prevention effect that 3 times fill after bacterium is 46.1%～47.8%.
The embodiment 7 bacillus amyloliquefaciens W118 fermentation liquid field control effect to early blight of tomato
Field test is carried out on Heng Xi proving ground, academy of agricultural sciences of Ningbo City, and test material is bacillus amyloliquefaciens W118 fermentation liquid, and test arranges 1.0 × 106cfu/ml、1.0×107cfu/ml、1.0×108cfu/ml、1.0×1094 Concentraton gradient of cfu/ml, use bacterial strain fermentation liquor after tomato seedling is transplanted 40 days, and concentration is 1.0 × 108Cfu/ml, uses 1 time every 7d, 3 times altogether.Every strain tomato seedling pouring 10ml, often processes 3 times and repeats, to use equivalent clear water for comparison.
Disease investigation: water bacterium 7d " Invest, Then Investigate " every time and record the incidence of each process tomato plant, adding up disease index and the prevention effect of each process.Severity Scaling standard is as follows: 0 grade: without scab；1 grade: lesion area≤25%；2 grades: 26%≤lesion area≤50%；3 grades: 51%≤lesion area≤75%；4 grades: lesion area >=76%.
Disease index=Σ (sick progression × this disease level plant strain number)/(serious disease progression × total strain number) × 100
Prevention effect (%)=(comparison disease index-process disease index)/comparison disease index × 100.
The table 4 bacillus amyloliquefaciens W118 fermentation liquid prevention effect to early blight of tomato
1) mean+SD.In same column, different lower cases represent notable at 0.05 level difference, alphabetical identical expression zero difference, and letter difference then represents variant.
From table 4, it can be seen that W118 fermentation liquid is also as the rising of W118 fermentation liquid concentration to the prevention effect of early blight of tomato and improves, and concentration is in 1.0 × 109Prevention effect after cfu/ml fills bacterium at 3 times is best.Wherein, it is 1.0 × 10 in concentration6Cfu/ml, the prevention effect that 3 times fill after bacterium is worst, and processes significant difference with other concentration, therefore, it is suggested that recommending concentration is 1.0 × 107～1.0 × 109Cfu/ml, the prevention effect that its 3 times fill after bacterium is 35.9%～43.4%.
The embodiment 8 bacillus amyloliquefaciens W118 field control effect to Leaf Spot Caused by Corynespora cassiicola on Cucumber
Field test is carried out on Heng Xi proving ground, academy of agricultural sciences of Ningbo City, and test material is W118 fermentation liquid, and test arranges 1.0 × 106cfu/ml、1.0×107cfu/ml、1.0×108cfu/ml、1.0×1094 Concentraton gradient of cfu/ml, transplant at cucumber seedling and use bacterial strain fermentation liquor in latter 45 days, use 1 time every 7d, 3 times altogether.Every strain cucumber seedling pouring 10ml, often processes 3 times and repeats, to use equivalent clear water for comparison.
Disease investigation: water bacterium 7d " Invest, Then Investigate " every time and record the incidence of each process cucumber plant, adding up disease index and the prevention effect of each process.The killed grade scale of blade: 0 grade: without scab；1 grade: lesion area≤5%；2 grades: 6%≤lesion area≤10%；3 grades: 11%≤lesion area≤20%；7 grades: 21%≤lesion area≤40%；9 grades: lesion area >=41%
Disease index=Σ (sick progression × this disease level plant strain number)/(serious disease progression × total strain number) × 100
Prevention effect (%)=(comparison disease index-process disease index)/comparison disease index × 100.
The table 5 bacillus amyloliquefaciens W118 fermentation liquid prevention effect to Leaf Spot Caused by Corynespora cassiicola on Cucumber
1) mean+SD.In same column, different lower cases represent notable at 0.05 level difference, alphabetical identical expression zero difference, and letter difference then represents variant.
As can be seen from Table 5, W118 fermentation liquid is also as the rising of W118 fermentation liquid concentration to the prevention effect of Leaf Spot Caused by Corynespora cassiicola on Cucumber and improves, and concentration is in 1.0 × 109Prevention effect after cfu/ml fills bacterium at 3 times is best, 1.0 × 107cfu/ml、1.0×108cfu/ml、1.0×109In cfu/ml these three concentration, prevention effect diversity is not notable, but is 1.0 × 10 in concentration6Cfu/ml, the prevention effect that 3 times fill after bacterium is worst, and processes significant difference with other concentration, therefore, it is suggested that recommending concentration is 1.0 × 107～1.0 × 109Cfu/ml, the prevention effect that its 3 times fill after bacterium is 33.1%～38.3%.
Claims (5)
1. a bacillus amyloliquefaciens W118, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) on March 10th, 2016, and deposit number is CGMCC No.12200.
2. the application of a bacillus amyloliquefaciens W118 according to claim 1, it is characterised in that the application to control of plant bacterial wilt.
3. the application of a bacillus amyloliquefaciens W118 according to claim 1, it is characterised in that to preventing and treating cotton verticillium wilt, the application of bacterium former to early blight of tomato and Leaf Spot Caused by Corynespora cassiicola on Cucumber.
4. the bacillus amyloliquefaciens W118 that a kind utilizes described in claim 2 application in preparing biocontrol agent, it is characterised in that: the effective dosage ranges of described biocontrol agent is 1.0 × 107～1.0 × 109cfu/ml。
5. the bacillus amyloliquefaciens W118 that a kind utilizes described in claim 3 application in preparing biocontrol agent, it is characterised in that: described anti-microbial inoculum is respectively 1.0 × 10 to cotton verticillium wilt, early blight of tomato, the effective dosage ranges of Leaf Spot Caused by Corynespora cassiicola on Cucumber8～1.0 × 109cfu/ml、1.0×107～1.0 × 109cfu/ml、1.0×107～1.0 × 109cfu/ml。
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| CN107365723A (en) * | 2017-07-13 | 2017-11-21 | 南京农业大学 | Chemicals and Biocontrol microorganism joint prevention and control continuous cropping tomato bacterial wilt |
| CN107858300A (en) * | 2017-05-31 | 2018-03-30 | 南京农业大学 | For the diseases prevention of tomato, growth-promoting, quality-improving and degeneration-resistant bacillus amyloliquefaciens 2YN11 and its application |
| CN108587986A (en) * | 2018-06-15 | 2018-09-28 | 保定微控生物科技有限公司 | A kind of bacillus amyloliquefaciens with diseases prevention and degrading organic phosphor double action |
| CN112391313A (en) * | 2020-11-19 | 2021-02-23 | 华南农业大学 | Bacillus amyloliquefaciens B86 and application thereof in preventing and treating fruit and vegetable bacterial wilt diseases |
| CN114958673A (en) * | 2022-06-07 | 2022-08-30 | 宁波市农业科学研究院 | Growth-promoting disease-preventing compound microbial agent and application thereof |
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| CN107858300A (en) * | 2017-05-31 | 2018-03-30 | 南京农业大学 | For the diseases prevention of tomato, growth-promoting, quality-improving and degeneration-resistant bacillus amyloliquefaciens 2YN11 and its application |
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| CN107365723B (en) * | 2017-07-13 | 2021-04-16 | 南京农业大学 | Chemical preparation and biocontrol microorganism combined control of tomato bacterial wilt disease |
| CN108587986A (en) * | 2018-06-15 | 2018-09-28 | 保定微控生物科技有限公司 | A kind of bacillus amyloliquefaciens with diseases prevention and degrading organic phosphor double action |
| CN108587986B (en) * | 2018-06-15 | 2021-06-01 | 齐永新 | Bacillus amyloliquefaciens with dual functions of preventing diseases and degrading organic phosphorus |
| CN112391313A (en) * | 2020-11-19 | 2021-02-23 | 华南农业大学 | Bacillus amyloliquefaciens B86 and application thereof in preventing and treating fruit and vegetable bacterial wilt diseases |
| CN114958673A (en) * | 2022-06-07 | 2022-08-30 | 宁波市农业科学研究院 | Growth-promoting disease-preventing compound microbial agent and application thereof |
| CN114958673B (en) * | 2022-06-07 | 2023-06-02 | 宁波市农业科学研究院 | Growth-promoting disease-preventing composite microbial agent and application thereof |
| CN115369062A (en) * | 2022-09-02 | 2022-11-22 | 上海市农业科学院 | Tomato bacterial wilt antagonistic bacterium WJB0802 and application thereof |
| CN115369062B (en) * | 2022-09-02 | 2023-10-20 | 上海市农业科学院 | Tomato bacterial wilt antagonistic bacterium WJB0802 and application thereof |
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